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these data suggest that phosphorylase activity in muscle cultured from patients with mcardle disease is due to a fetal isoenzyme whose genetic control is different from that of the mature enzyme .
NA
{ "id": "C0022173", "name": "isoenzyme", "pos": [ 119, 128 ] }
{ "id": "C0014442", "name": "enzyme", "pos": [ 188, 194 ] }
antiviral activity of the complexes of synthetic polyribonucleotides , i.e . poly ( i ) .poly ( c ) and poly ( g ) .poly ( c ) obtained at non-equimolar ratios of homopolymers was studied .
NA
{ "id": "C0032590", "name": "polyribonucleotides", "pos": [ 49, 68 ] }
{ "id": "C0032411", "name": "poly", "pos": [ 167, 171 ] }
the wine yeasts cheres were not sensitive to high concentration ( 500 gamma/ml ) of benzylpenicillin , streptomycin , tetracycline , oxytetracycline , chlortetracycline , morphocycline , erythromycin , oleandomycin , monomycin , neomycin , kanamycin , polymyxin , ampicillin , oxacillin , methicillin , ceporin , ristomycin , levomycetin , furadonin and furazolidone .
NA
{ "id": "C0030827", "name": "benzylpenicillin", "pos": [ 84, 100 ] }
{ "id": "C0002680", "name": "ampicillin", "pos": [ 264, 274 ] }
benzylpenicillin , polymyxin , neomycin and ampicillin in concentrations of 50 to 100 gamma/ml had a stimulating effect on the yeast biomass synthesis .
NA
{ "id": "C0030827", "name": "benzylpenicillin", "pos": [ 0, 16 ] }
{ "id": "C0002680", "name": "ampicillin", "pos": [ 44, 54 ] }
higher toxicity of carfecillin as compared to carbenicillin may be due to production of free phenol on carfecillin hydrolysin in the animal organism .
NA
{ "id": "C0006976", "name": "carbenicillin", "pos": [ 46, 59 ] }
{ "id": "C0007241", "name": "carfecillin", "pos": [ 103, 114 ] }
only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 91, 102 ] }
{ "id": "C0002521", "name": "branched-chain amino", "pos": [ 128, 148 ] }
the cis and trans isomers of zearalenone [ 2,4-dihyroxy-6- ( 10-hydroxy-6-oxo-1-undecenyl ) -benzoic acid mu-lactone ] and zearalenol [ 2,4-dihydroxy-6- ( 6,10-dihydroxy-1-undecenyl ) -benzoic acid mu-lactone ] were tested for uterotropic activity in the white rat .
NA
{ "id": "C0043454", "name": "zearalenone", "pos": [ 29, 40 ] }
{ "id": "C0022947", "name": "lactone", "pos": [ 201, 208 ] }
it is shown that daily oral administration of 5 -- 10 mg of an antidiabetic biguanide-phenformin ( phenethyl-biguanide ) for 2.5 -- 5 months suppressed dmba-induced mammary tumour development in rats considerably .
NA
{ "id": "C0005382", "name": "biguanide", "pos": [ 76, 85 ] }
{ "id": "C0031404", "name": "phenformin", "pos": [ 86, 96 ] }
fragmentation and degeneration of the elastic laminae of muscular arteries was followed by vascular calcification that could not be distinguished morphologically from monckeberg 's arteriosclerosis .
NA
{ "id": "C0342649", "name": "vascular calcification", "pos": [ 91, 113 ] }
{ "id": "C0887866", "name": "monckeberg 's arteriosclerosis", "pos": [ 167, 197 ] }
it is suggested that the selective accumulation of salicylates in the parietal cells may account for the specific cytotoxic actions of aspirin on these cells .
NA
{ "id": "C0036077", "name": "salicylates", "pos": [ 51, 62 ] }
{ "id": "C0004057", "name": "aspirin", "pos": [ 135, 142 ] }
when used in conjunction with timolol , other adrenergic amines , such as norepinephrine and epinephrine , produce an additional hypotensive response .
NA
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 74, 88 ] }
{ "id": "C0014563", "name": "epinephrine", "pos": [ 93, 104 ] }
pathophysiology and tomography of the blood vessels of the posterior cranial fossa gain clinical interest in treatment of diseases of the inner ear , complications of middle ear inflammations , tumors of the pyramid and cerebello-pontine angle .
NA
{ "id": "C0013447", "name": "of diseases", "pos": [ 119, 130 ] }
{ "id": "C0029877", "name": "of", "pos": [ 201, 203 ] }
physiological measurements consisted of heart rate and urine biochemical analysis for 17-ketogenic steroids , epinephrine , and norepinephrine .
NA
{ "id": "C0014563", "name": "epinephrine", "pos": [ 110, 121 ] }
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 128, 142 ] }
at sacrifice , variations in heart weight were evaluated relative to sex , initial body weight , relative change in body weight and level of treatment .
NA
{ "id": "C0005910", "name": "weight", "pos": [ 35, 41 ] }
{ "id": "C0005911", "name": "change in", "pos": [ 106, 115 ] }
both enzymes hydrolysed other short-chain acyl-coa compounds and succinyl-coa ( 3-carboxypropionyl-coa ) , but with one acetyl-coa was the preferred substrate .
NA
{ "id": "C0001374", "name": "acyl-coa", "pos": [ 42, 50 ] }
{ "id": "C0001026", "name": "acetyl-coa", "pos": [ 120, 130 ] }
rrna from detergent-purified nuclei was fractionated quantitatively , by two independent methods , into nucleolar and nucleoplasmic rna fractions .
NA
{ "id": "C0035701", "name": "rrna", "pos": [ 0, 4 ] }
{ "id": "C0035668", "name": "rna", "pos": [ 132, 135 ] }
the two rna fractions were analysed by urea/agar-gel electrophoresis and the amount of pre-rrna ( precursor of rrna ) and rrna components was determined .
NA
{ "id": "C0035668", "name": "rna", "pos": [ 8, 11 ] }
{ "id": "C0035701", "name": "rrna", "pos": [ 122, 126 ] }
the rrna constitutes 35 % of total nuclear rna , of which two-thirds are in nucleolar rna and one-third in nucleoplasmic rna .
NA
{ "id": "C0035701", "name": "rrna", "pos": [ 4, 8 ] }
{ "id": "C0035668", "name": "rna", "pos": [ 121, 124 ] }
the rrna constitutes 35 % of total nuclear rna , of which two-thirds are in nucleolar rna and one-third in nucleoplasmic rna .
NA
{ "id": "C0035700", "name": "nuclear rna", "pos": [ 35, 46 ] }
{ "id": "C0035668", "name": "rna", "pos": [ 121, 124 ] }
the bulk of rrna in nucleoplasmic rna is represented by 28 s and 18 s rrna in a molar ratio close to 1.0 .
NA
{ "id": "C0035668", "name": "rna", "pos": [ 13, 16 ] }
{ "id": "C0035701", "name": "rrna", "pos": [ 70, 74 ] }
part of the mature rrna species in nucleoplasmic rna originate from ribosomes attached to the outer nuclear membrane , which resist detergent treatment .
NA
{ "id": "C0035701", "name": "rrna", "pos": [ 19, 23 ] }
{ "id": "C0035668", "name": "rna", "pos": [ 49, 52 ] }
scallop troponin i was isolated and mixed with chicken troponin c and troponin t , forming a functional hybrid troponin complex , indicating that scallop and vertebrate troponins may act by a common mechanism .
NA
{ "id": "C0077401", "name": "troponin i", "pos": [ 8, 18 ] }
{ "id": "C0041199", "name": "troponin", "pos": [ 169, 177 ] }
scallop troponin i was isolated and mixed with chicken troponin c and troponin t , forming a functional hybrid troponin complex , indicating that scallop and vertebrate troponins may act by a common mechanism .
NA
{ "id": "C0077404", "name": "troponin t", "pos": [ 70, 80 ] }
{ "id": "C0041199", "name": "troponin", "pos": [ 169, 177 ] }
their amino acid compositions are very similar , both being high in aspartic acid , glutamic acid , serine , glycine and leucine , and low in methionine , tryptophan , tyrosine , isoleucine and histidine .
NA
{ "id": "C0002520", "name": "amino acid", "pos": [ 6, 16 ] }
{ "id": "C0017890", "name": "glycine", "pos": [ 109, 116 ] }
the ph optimum for the enzyme was ph 7.5 , and it degraded linear-duplex dna or closed-circular-duplex dna to acid-soluble material .
NA
{ "id": "C0012854", "name": "dna", "pos": [ 73, 76 ] }
{ "id": "C0012925", "name": "circular", "pos": [ 87, 95 ] }
however , dichloromethanediphosphonate stopped the growth of the calvaria cells and the fibroblasts after the beginning of confluence , whereas the untreated cells were still growing to a certain extent .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 88, 99 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 158, 163 ] }
radioactive dichloromethanediphosphonate and 1-hydroxyethane-1,1-diphosphonate were taken up linearly with time for at least 48 h by calvaria cells and fibroblasts .
NA
{ "id": "C0007634", "name": "cells", "pos": [ 142, 147 ] }
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 152, 163 ] }
a rapid , sensitive fluorometric method for the determination of ajmaline and n-propylajmaline ( npa ) was used to follow serum concentrations after an i.v . administration of ajmaline , after an oral administration of ajmaline , and an oral dose of npa bitartrate ( npab ) in beagle dogs .
NA
{ "id": "C0001888", "name": "ajmaline", "pos": [ 65, 73 ] }
{ "id": "C0032899", "name": "npa", "pos": [ 267, 270 ] }
ajmaline was eliminated from serum with an apparent half-life of approximately 1 h and npa with one of approximately 4 h .
NA
{ "id": "C0001888", "name": "ajmaline", "pos": [ 0, 8 ] }
{ "id": "C0032899", "name": "npa", "pos": [ 87, 90 ] }
the peak serum concentrations of ajmaline and npa after a single oral dose of 50 mg of ajmaline and npab were about 1.4 microgram/ml and 0.6 microgram/ml , respectively , the latter calculated as its bitartrate , with about the same peak time : 1 h .
NA
{ "id": "C0032899", "name": "npa", "pos": [ 46, 49 ] }
{ "id": "C0001888", "name": "ajmaline", "pos": [ 87, 95 ] }
in rabbits undergoing active-passive immunization , the appearance time of endogenous antibodies is delayed , all the more so as the antiserum injected dose increases .
NA
{ "id": "C0003241", "name": "antibodies", "pos": [ 86, 96 ] }
{ "id": "C0020960", "name": "antiserum", "pos": [ 133, 142 ] }
owing to the close relationship between ionizing raditions and chromosome aberrations on one hand , and abnormal karyotypes and tumors on the other , it can not be overlooked that these aberrations may play an important role , together with other carcinogenic factors , in the development of second malignancies in these patients .
NA
{ "id": "C0027651", "name": "tumors", "pos": [ 128, 134 ] }
{ "id": "C0085183", "name": "second malignancies", "pos": [ 292, 311 ] }
chronological endocrine changes accompanying various diseases in the hypothalamo-pituitary region ( 183 cases ) were collectively studied by evaluating both endocrinological symptoms and anterior pituitary functions , with the following results : 1 ) endocrine disorders following the operation , trauma and subarachnoideal hemorrhage in the pituitary-diencephalic region varied in the order of a ) subclinical , b ) clinical and c ) paradoxical .
NA
{ "id": "C0032002", "name": "diseases", "pos": [ 53, 61 ] }
{ "id": "C0014130", "name": "endocrine disorders", "pos": [ 251, 270 ] }
4 ) the endocrine disorder developed and exacerbated in 6 months to 2 years after radio therapy of the pituitary-diencephalic region .
NA
{ "id": "C0014130", "name": "endocrine disorder", "pos": [ 8, 26 ] }
{ "id": "C0032002", "name": "pituitary", "pos": [ 103, 112 ] }
spleen cells from adult mice which had been thymectomized 8 weeks previously demonstrated a severely impaired primary cytotoxic response to p815 tumour cells , whereas their cytotoxic responses to allogeneic cells ( c57bl/6 ) and to non-h-2 antigens ( balb/c ) , and their ability to form a primary antibody response to sheep red blood cells was unimpaired .
NA
{ "id": "C0007634", "name": "cells", "pos": [ 7, 12 ] }
{ "id": "C3485979", "name": "allogeneic cells", "pos": [ 197, 213 ] }
the salient features of this method for biological monitoring of occupational exposure to organophosphorus insecticides are : ( a ) acetylcholinesterase ( ache ) and butyrylcholinesterase ( buche ) are determined separately in whole haemolysed blood using specific substrates at appropriate concentrations ;
NA
{ "id": "C0001044", "name": "acetylcholinesterase", "pos": [ 132, 152 ] }
{ "id": "C0008429", "name": "butyrylcholinesterase", "pos": [ 166, 187 ] }
apart from isolated ectopic beats , disturbances of rhythm were uncommon , and neither vpb nor other arrhythmias appeared to be induced by occupational factors .
NA
{ "id": "C0340464", "name": "ectopic beats", "pos": [ 20, 33 ] }
{ "id": "C0151636", "name": "vpb", "pos": [ 87, 90 ] }
apart from isolated ectopic beats , disturbances of rhythm were uncommon , and neither vpb nor other arrhythmias appeared to be induced by occupational factors .
NA
{ "id": "C0340464", "name": "ectopic beats", "pos": [ 20, 33 ] }
{ "id": "C0003811", "name": "arrhythmias", "pos": [ 101, 112 ] }
the effect of long-term application of 4 % atenolol eye drops has been studied in patients with either glaucoma or ocular hypertension .
NA
{ "id": "C0017601", "name": "glaucoma", "pos": [ 103, 111 ] }
{ "id": "C0028840", "name": "ocular hypertension", "pos": [ 115, 134 ] }
the addition of h2o2 to superoxide dismutase reduces the copper to cu ( i ) , while oxygen addition to the peroxide-reduced protein restores the copper to cu ( ii ) .
NA
{ "id": "C0020281", "name": "h2o2", "pos": [ 16, 20 ] }
{ "id": "C0031180", "name": "peroxide", "pos": [ 107, 115 ] }
analogue binding to egg white binding apoprotein has been quantitated and serves to determine the origins of binding site specificity for this protein .
NA
{ "id": "C0003601", "name": "apoprotein", "pos": [ 38, 48 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 143, 150 ] }
this suggests a common mechanism for the reactions of such diverse flavins as riboflavin , 5-deazariboflavin , and 1-deazariboflavin .
NA
{ "id": "C0016213", "name": "flavins", "pos": [ 67, 74 ] }
{ "id": "C0035527", "name": "riboflavin", "pos": [ 122, 132 ] }
the interactions of 4-nitroquinoline 1-oxide ( nqo ) with the four 5'-deoxyribonucleotides were probed using absorption spectra of the charge transfer bands and 1h and 13c nuclear magnetic resonance ( nmr ) spectra of nucleotide-nqo mixtures .
NA
{ "id": "C0011529", "name": "deoxyribonucleotides", "pos": [ 70, 90 ] }
{ "id": "C0028630", "name": "nucleotide", "pos": [ 218, 228 ] }
furthermore , we conclude that the interactions of polymerase with ss dna are , in general , stronger than the nonspecific interations of the enzyme with ds dna .
NA
{ "id": "C0012935", "name": "ss dna", "pos": [ 67, 73 ] }
{ "id": "C0012854", "name": "ds dna", "pos": [ 154, 160 ] }
such spectra have been recorded in aqueous solutions under conditions allowing a study of the exchangeable proton resonances of histone complexes for the first time and indicate that the structured regions are rich in hydrophobic amino acids , as well as arginine and some acidic amino acids .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 230, 241 ] }
{ "id": "C0815040", "name": "acidic amino acids", "pos": [ 273, 291 ] }
transfer of anionic liposomes prepared at a given ph to a solution of different ph resulted in a ph gradient if sodium phosphate or borate were used as buffers .
NA
{ "id": "C0003075", "name": "anionic", "pos": [ 12, 19 ] }
{ "id": "C0006007", "name": "borate", "pos": [ 132, 138 ] }
( 2 ) the antibiotics rutamycin , dio-9 , quercetin , oligomycin , botrycidin , efrapeptin , leucinostatin , valinomycin , and venturicidin as well as n , n'-dicyclohexylcarbodiimide and dinitrophenol are potent inhibitors of f0.f1atpase activity . ( 3 ) f0.f1atpase activity is completely inhibited by anti-f1atpase antibodies .
NA
{ "id": "C0035972", "name": "rutamycin", "pos": [ 22, 31 ] }
{ "id": "C0028951", "name": "oligomycin", "pos": [ 54, 64 ] }
a method is presented for the separation of 6-thiopurine bases and ribonucleosides , of sulphate anions and of common purine bases and oxidized purines by means of high-pressure liquid cation-exchange chromatography using a 0.18 x 100 cm column , filled with beckman m71 resin , and eluted with 0.4m ammonium formate , ph 4.6 , at a linear flow velocity of 5.2 cm/min at 50 degrees c .
NA
{ "id": "C0038720", "name": "sulphate", "pos": [ 88, 96 ] }
{ "id": "C0003075", "name": "anions", "pos": [ 97, 103 ] }
it was determined that of six tetracyclines tested , four strongly inhibited the reaction ( oxytetracycline , chlorotetracycline , dedimethylaminotetracycline , and tetracycline ) whereas 5a,6-anhydrotetracycline gave a moderate inhibition and alpha-6-deoxyoxytetracycline resulted in only a slight reduction in ppgpp synthesis .
NA
{ "id": "C0039651", "name": "tetracyclines", "pos": [ 30, 43 ] }
{ "id": "C0030092", "name": "oxytetracycline", "pos": [ 257, 272 ] }
it was determined that of six tetracyclines tested , four strongly inhibited the reaction ( oxytetracycline , chlorotetracycline , dedimethylaminotetracycline , and tetracycline ) whereas 5a,6-anhydrotetracycline gave a moderate inhibition and alpha-6-deoxyoxytetracycline resulted in only a slight reduction in ppgpp synthesis .
NA
{ "id": "C0039651", "name": "tetracyclines", "pos": [ 30, 43 ] }
{ "id": "C0008293", "name": "chlorotetracycline", "pos": [ 110, 128 ] }
it was determined that of six tetracyclines tested , four strongly inhibited the reaction ( oxytetracycline , chlorotetracycline , dedimethylaminotetracycline , and tetracycline ) whereas 5a,6-anhydrotetracycline gave a moderate inhibition and alpha-6-deoxyoxytetracycline resulted in only a slight reduction in ppgpp synthesis .
NA
{ "id": "C0039651", "name": "tetracyclines", "pos": [ 30, 43 ] }
{ "id": "C0013090", "name": "alpha-6-deoxyoxytetracycline", "pos": [ 244, 272 ] }
at this ionic strength the secondary structure of double-stranded rna is maintained , as judged by the following : ( a ) the unchanged resistance of double-stranded rna and dna , under similar low ionic strength conditions , to nuclease s1 from aspergillus oryzae , in contrast with the sensitivity of the corresponding denatured nucleic acids to this enzyme , specific for single-stranded rna and dna ;
NA
{ "id": "C0028606", "name": "nucleic acids", "pos": [ 330, 343 ] }
{ "id": "C0012854", "name": "dna", "pos": [ 398, 401 ] }
whereas in ssc bovine seminal rnaase ( rnaase bs-1 ) and whale pancreatic rnaase show an activity on double-stranded rna significantly higher than that of rnaase a , in 0.1 x ssc the activity of the latter enzyme on this substrate becomes distinctly higher than that of rnaase bs-1 , and similar to that of whale rnaase .
NA
{ "id": "C0035541", "name": "pancreatic rnaase", "pos": [ 63, 80 ] }
{ "id": "C0014241", "name": "rnaase", "pos": [ 313, 319 ] }
these oligonucleotides were fractionated first be deae-cellulose chromatography with 7 m urea into the respective isoplithic groups and then analysed for the composition and isomerism : each group was labeled 5'-terminally by the [ gamma-32p ] atp-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-dimensionally for the di- and trinucleotides mixtures , respectively , followed by elution and digestion with snake venom exonuclease .
NA
{ "id": "C0028953", "name": "oligonucleotides", "pos": [ 6, 22 ] }
{ "id": "C0032550", "name": "polynucleotide", "pos": [ 248, 262 ] }
these included a reduction in the amounts of adenosine and 3- ( 3-amino-3-carboxypropyl ) -uridine , and an increase in the amounts of 1-methyl-adenosine , 1-methylguanosine , 3-methylcytidine and pseudouridine .
NA
{ "id": "C0041984", "name": "uridine", "pos": [ 91, 98 ] }
{ "id": "C0033846", "name": "pseudouridine", "pos": [ 197, 210 ] }
as compared to 8-methoxypsoralen , a well-known bifunctional furocoumarin , 3-carbethoxypsoralen exhibits a high photoaffinity for dna in vivo .
NA
{ "id": "C0025684", "name": "8-methoxypsoralen", "pos": [ 15, 32 ] }
{ "id": "C0016859", "name": "furocoumarin", "pos": [ 61, 73 ] }
the time required for one translocation by all active ribosomes , and the time required for the incorporation of one amino acid ( starting with aminoacyl-trna or amino acids ) has also been determined .
NA
{ "id": "C0035714", "name": "aminoacyl-trna", "pos": [ 144, 158 ] }
{ "id": "C0002520", "name": "amino acids", "pos": [ 162, 173 ] }
the amino acid composition of hydrogenase preparations from different fractions of t. roseopersicina is identical , glycine and alanine as n-terminal amino acid residues .
NA
{ "id": "C0002520", "name": "amino acid", "pos": [ 4, 14 ] }
{ "id": "C0017890", "name": "glycine", "pos": [ 116, 123 ] }
the amino acid composition of hydrogenase preparations from different fractions of t. roseopersicina is identical , glycine and alanine as n-terminal amino acid residues .
NA
{ "id": "C0002520", "name": "amino acid", "pos": [ 4, 14 ] }
{ "id": "C0001898", "name": "alanine", "pos": [ 128, 135 ] }
an increased low km cyclic amp phosphodiesterase activity was found upon exposure of intact fibroblasts to 3-isobutyl-1-methylxanthine , an inhibitor of phosphodiesterase activity in broken cell preparations , as well as to other agents which elevate cyclic amp levels in these cells .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 92, 103 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 278, 283 ] }
form b2 is the major form of the aromatic-amino-acid aminotransferase ( aromatic-amino-acid:2-oxoglutarate amino-transferase , ec 2.6.1.57 ) and the km values of tyrosine and phenylalanine with this form are somewhat lower than with the minor form a .
NA
{ "id": "C0301713", "name": "aromatic-amino-acid", "pos": [ 33, 52 ] }
{ "id": "C0041485", "name": "tyrosine", "pos": [ 162, 170 ] }
form b2 is the major form of the aromatic-amino-acid aminotransferase ( aromatic-amino-acid:2-oxoglutarate amino-transferase , ec 2.6.1.57 ) and the km values of tyrosine and phenylalanine with this form are somewhat lower than with the minor form a .
NA
{ "id": "C0301713", "name": "aromatic-amino-acid", "pos": [ 33, 52 ] }
{ "id": "C0031453", "name": "phenylalanine", "pos": [ 175, 188 ] }
the km of tyrosine with histidinol phosphate aminotransferase ( protein b1 ) is in the same range , but the km of phenylalanine with this enzyme is 12-20 times higher than the corresponding values with the two forms of the aromatic-amino-acid amino-transferase .
NA
{ "id": "C0041485", "name": "tyrosine", "pos": [ 10, 18 ] }
{ "id": "C0301713", "name": "aromatic-amino-acid", "pos": [ 223, 242 ] }
the km of tyrosine with histidinol phosphate aminotransferase ( protein b1 ) is in the same range , but the km of phenylalanine with this enzyme is 12-20 times higher than the corresponding values with the two forms of the aromatic-amino-acid amino-transferase .
NA
{ "id": "C0031453", "name": "phenylalanine", "pos": [ 114, 127 ] }
{ "id": "C0301713", "name": "aromatic-amino-acid", "pos": [ 223, 242 ] }
the dehydrogenase may be of importance for the regulation of the level of endogenous prostaglandins and , thus , a decrease in activity could result in a slower turnover of prostaglandins .
NA
{ "id": "C0033554", "name": "prostaglandins", "pos": [ 85, 99 ] }
{ "id": "C0033555", "name": "a", "pos": [ 181, 182 ] }
the degradation of 125i-labeled low density lipoprotein by cultured human skin fibroblasts was enhanced 25 % by preincubation of cells with insulin .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 79, 90 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 129, 134 ] }
proteolysis of the lipoproteins by agarose-trypsin was more rapid at 37 degrees c than at 22 degrees c , but the proteolytic products were similar and differed from the products from the lipid free proteins .
NA
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 19, 31 ] }
{ "id": "C0023779", "name": "lipid", "pos": [ 187, 192 ] }
peptide fragments from lipoproteins were larger than those from lipid-free proteins , which suggests masking of potentially cleavable groups by lipid .
NA
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 23, 35 ] }
{ "id": "C0023779", "name": "lipid", "pos": [ 144, 149 ] }
the amounts ( mol/g protein ) of new carboxyl-terminal tyrosine and phenylalanine released by agarose -chymotrypsin were much greater from the lipid-free proteins , but about 3/4 of the tryptophan residues were inacessible in both lipoproteins and lipid-free proteins .
NA
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 231, 243 ] }
{ "id": "C0023779", "name": "lipid", "pos": [ 248, 253 ] }
in agarose-trypsin digestion , lysine residues were slightly more masked than arginine in the absence of lipids and much more so in the lipoproteins .
NA
{ "id": "C0023779", "name": "lipids", "pos": [ 105, 111 ] }
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 136, 148 ] }
the results suggest that ldl apoprotein may be made of segments of , at least , very similar amino acid composition and that both the protein itself and derivative fragments have a great tendency to aggregate even in denaturing solvents .
NA
{ "id": "C0003601", "name": "apoprotein", "pos": [ 29, 39 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 134, 141 ] }
the large subunit of fraction 1 protein from lycopersicon esculentum , nicotiana tabacum and petunia hybrida has been examined by isoelectric focusing of the s-carboxymethylated polypeptides , and by double immunodiffusion with antiserum raised against fraction 1 protein .
NA
{ "id": "C0599220", "name": "subunit", "pos": [ 10, 17 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 264, 271 ] }
the ratio of the acidic amino acids and their amides ( glx plus asx ) to the basic amino acids ( lys plus arg ) was 2.2 .
NA
{ "id": "C0301714", "name": "basic amino acids", "pos": [ 77, 94 ] }
{ "id": "C0024337", "name": "lys", "pos": [ 97, 100 ] }
of the other calcium-binding proteins examined , namely , troponin-c , s-100 protein , phospholipase a , phospholipase b and myosin light chain , only troponin-c displayed any significant calcium-specific binding of trifluoperazine .
NA
{ "id": "C0006732", "name": "calcium-binding proteins", "pos": [ 13, 37 ] }
{ "id": "C0027108", "name": "myosin light chain", "pos": [ 125, 143 ] }
of the other calcium-binding proteins examined , namely , troponin-c , s-100 protein , phospholipase a , phospholipase b and myosin light chain , only troponin-c displayed any significant calcium-specific binding of trifluoperazine .
NA
{ "id": "C0006732", "name": "calcium-binding proteins", "pos": [ 13, 37 ] }
{ "id": "C0077400", "name": "troponin-c", "pos": [ 151, 161 ] }
inhibition of the cervical cell activity by diisopropylfluorophosphate and p-nitrophenyl-p'-guanidinobenzoate demonstrates that , like urokinase and trypsin , this plasminogen activator is also a serine protease .
NA
{ "id": "C0041236", "name": "trypsin", "pos": [ 149, 156 ] }
{ "id": "C0036734", "name": "serine protease", "pos": [ 196, 211 ] }
both bacteriocins like wild-type cloacin df13 , are still able to inhibit in vitro protein synthesis , but their in vivo killing activity is absent .
NA
{ "id": "C0004641", "name": "wild-type", "pos": [ 23, 32 ] }
{ "id": "C0008988", "name": "cloacin", "pos": [ 33, 40 ] }
it has been shown that ca2+-atpase possesses the same mobility as skeletal muscle myosin under gel chromatography on sepharose 4b and the same mobility as myosin heavy chains in sodium dodecyl sulfate -- polyacrylamide gel electrophoresis .
NA
{ "id": "C0027096", "name": "myosin", "pos": [ 82, 88 ] }
{ "id": "C0027100", "name": "myosin heavy chains", "pos": [ 155, 174 ] }
comparative analysis of the lytic effect of anionic , cationic and non-ionic sac is carried out , a correlation between key physico-chemical characteristics of the agents ( critical concentration of micellization , hydrophyle-lipophyle balance ) and their lytic activity is investigated .
NA
{ "id": "C0003075", "name": "anionic", "pos": [ 44, 51 ] }
{ "id": "C0022023", "name": "ionic", "pos": [ 71, 76 ] }
comparative analysis of the lytic effect of anionic , cationic and non-ionic sac is carried out , a correlation between key physico-chemical characteristics of the agents ( critical concentration of micellization , hydrophyle-lipophyle balance ) and their lytic activity is investigated .
NA
{ "id": "C0007447", "name": "cationic", "pos": [ 54, 62 ] }
{ "id": "C0022023", "name": "ionic", "pos": [ 71, 76 ] }
the mg- and ca-atpase activities of the wheat embryo nuclei were found in the nuclear fraction of albumin , in nonhistone proteins and nuclear membranes .
NA
{ "id": "C0001924", "name": "albumin", "pos": [ 98, 105 ] }
{ "id": "C0033684", "name": "proteins", "pos": [ 122, 130 ] }
in the albumin nuclear fraction and subfractions of non-histone proteins the higher level of activity is observed in ca-atpase , whereas in the nuclei and soluble fractions of residual proteins in mg-atpase .
NA
{ "id": "C0001924", "name": "albumin", "pos": [ 7, 14 ] }
{ "id": "C0033684", "name": "proteins", "pos": [ 185, 193 ] }
the replacement of nh-group to the c-end of the angiotensin molecule by affection the antigen determinant considerably decreases the antigen activity of the analogues .
NA
{ "id": "C0003316", "name": "antigen determinant", "pos": [ 86, 105 ] }
{ "id": "C0003320", "name": "antigen", "pos": [ 133, 140 ] }
paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state ( methemoglobin ) .
NA
{ "id": "C0019046", "name": "hemoglobin", "pos": [ 89, 99 ] }
{ "id": "C0025635", "name": "methemoglobin", "pos": [ 122, 135 ] }
the endonuclease-sensitive site and tlc measurements can be reconciled by the assumption that pyrimidine dimers are excised from high molecular weight dna in acid-insoluble oligonucleotides that are slowly degraded to acid-soluble fragments .
NA
{ "id": "C0034286", "name": "pyrimidine dimers", "pos": [ 94, 111 ] }
{ "id": "C0028953", "name": "oligonucleotides", "pos": [ 173, 189 ] }
identification of sources of hydrogen atoms in fatty acids synthesized using deuterated water and stereospecifically deuterium labelled nadph by gas chromatographic mass spectrometric analysis .
NA
{ "id": "C0020275", "name": "hydrogen", "pos": [ 29, 37 ] }
{ "id": "C0011744", "name": "deuterium", "pos": [ 117, 126 ] }
precursors of the stromal fibroblasts in native cell suspensions were found to be essentially more sensitive to the cytotoxic action of afs than the colony-forming fibroblasts on the passage cultures .
NA
{ "id": "C0007634", "name": "cell", "pos": [ 48, 52 ] }
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 164, 175 ] }
[ effect of mouse antiserum against isologous aggregated immunoglobulins on the accumulation of rosette- and antibody-forming cells in mice immunized with ram erythrocytes ] .
NA
{ "id": "C0020960", "name": "antiserum", "pos": [ 18, 27 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 109, 117 ] }
the paper describes the effect of mouse antiserum against isologous aggregated immunoglobulins ( termed maas ) on the kinetics of rosette-forming and antibody-forming cells ( rfc and afc , respectively ) in mice immunized with srbc .
NA
{ "id": "C0020960", "name": "antiserum", "pos": [ 40, 49 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 150, 158 ] }
the rate of 3h-glycine incorporation into the total proteins of the liver , those of microsomal fraction , proteins of hyaloplasm , and plasmatic membrane proteins , soluble and non-soluble in 0.05 m k2co3 , was determined .
NA
{ "id": "C0033684", "name": "proteins", "pos": [ 52, 60 ] }
{ "id": "C0025252", "name": "membrane proteins", "pos": [ 146, 163 ] }
1 pretreatment of rats with intraperitoneal injections of lead was shown to result in a depression of the microsomal mixed function oxidase system , as assessed by a decrease in hepatic microsomal p-450 and b5 content and by a decrease in the activity of the enzymes aniline hydroxylase and aminopyrine demethylase .
NA
{ "id": "C0003927", "name": "microsomal mixed function oxidase", "pos": [ 106, 139 ] }
{ "id": "C0003039", "name": "aniline hydroxylase", "pos": [ 267, 286 ] }
1 the activity produced by the adenine nucleotides adenosine triphosphate ( atp ) , adenosine diphosphate ( adp ) and adenosine monophosphate ( amp ) and by potassium , acetylcholine ( ach ) , 5-hydroxytryptamine ( 5-ht ) and bradykinin when applied to an exposed blister base on the ear of anaesthetized rabbits or intra-arterially to anaesthetized cats was investigated in multiple strands dissected from the auricular-temporal and saphenous nerves of rabbits and cats , respectively .
NA
{ "id": "C0001413", "name": "adenine nucleotides", "pos": [ 31, 50 ] }
{ "id": "C0001480", "name": "adenosine triphosphate", "pos": [ 51, 73 ] }
1 the activity produced by the adenine nucleotides adenosine triphosphate ( atp ) , adenosine diphosphate ( adp ) and adenosine monophosphate ( amp ) and by potassium , acetylcholine ( ach ) , 5-hydroxytryptamine ( 5-ht ) and bradykinin when applied to an exposed blister base on the ear of anaesthetized rabbits or intra-arterially to anaesthetized cats was investigated in multiple strands dissected from the auricular-temporal and saphenous nerves of rabbits and cats , respectively .
NA
{ "id": "C0001413", "name": "adenine nucleotides", "pos": [ 31, 50 ] }
{ "id": "C0001459", "name": "adenosine diphosphate", "pos": [ 84, 105 ] }
1 the activity produced by the adenine nucleotides adenosine triphosphate ( atp ) , adenosine diphosphate ( adp ) and adenosine monophosphate ( amp ) and by potassium , acetylcholine ( ach ) , 5-hydroxytryptamine ( 5-ht ) and bradykinin when applied to an exposed blister base on the ear of anaesthetized rabbits or intra-arterially to anaesthetized cats was investigated in multiple strands dissected from the auricular-temporal and saphenous nerves of rabbits and cats , respectively .
NA
{ "id": "C0001413", "name": "adenine nucleotides", "pos": [ 31, 50 ] }
{ "id": "C0001465", "name": "adenosine monophosphate", "pos": [ 118, 141 ] }
the condition may present with jaundice , malabsorption or the signs and symptoms of intestinal obstruction due to internal hernia or volvulus .
NA
{ "id": "C0021843", "name": "of intestinal obstruction", "pos": [ 82, 107 ] }
{ "id": "C0042961", "name": "volvulus", "pos": [ 134, 142 ] }
under the experimental conditions used , norepinephrine as well as alpha-methyl-norepinephrine were taken up in only noradrenergic and dopaminergic terminals .
NA
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 41, 55 ] }
{ "id": "C0025805", "name": "alpha-methyl-norepinephrine", "pos": [ 67, 94 ] }
the effects of some amino acids ( glu , gly and gaba ) , applied in 3 different manners ( electrophoretically , in the superfusate and by pressure-microinjection ) , were investigated on spontaneous and light-induced spike discharges in the isolated carp retina .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 20, 31 ] }
{ "id": "C0017890", "name": "gly", "pos": [ 40, 43 ] }
a microiontophoretic study was performed of the actions of angiotensin ii and angiotensin fragments on neurones of the subfornical organ ( sfo ) .
NA
{ "id": "C0003009", "name": "angiotensin ii", "pos": [ 59, 73 ] }
{ "id": "C0003018", "name": "angiotensin", "pos": [ 78, 89 ] }
( 3 ) the involvement of other catecholaminergic systems was excluded by pretreatment of the rats with reserpine plus a-methyl-p-tyrosine which reduced the levels of na , dopamine and adrenaline in the frontal cortex with 92 , 97 and 99 % respectively .
NA
{ "id": "C0028351", "name": "na", "pos": [ 166, 168 ] }
{ "id": "C0014563", "name": "adrenaline", "pos": [ 184, 194 ] }
serum epinephrine and norepinephrine were measured in arterial samples drawn pre-induction , post-induction , before cardiopulmonary bypass , two and eight minutes after initiation of cardiopulmonary bypass , 20 minutes before termination and after termination of cardiopulmonary bypass .
NA
{ "id": "C0014563", "name": "epinephrine", "pos": [ 6, 17 ] }
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 22, 36 ] }