text stringlengths 57 1.72k | relation stringclasses 125
values | h dict | t dict |
|---|---|---|---|
because camp analogs decreased androgen and estrogen receptor concentrations , the effect of calcitonin on sex steroid receptors may not be mediated by its effect on cyclic amp in these cells . | NA | {
"id": "C0034804",
"name": "estrogen receptor",
"pos": [
44,
61
]
} | {
"id": "C0034840",
"name": "steroid receptors",
"pos": [
111,
128
]
} |
patients presented with systemic infection ( 58 ) , pneumonia ( 58 ) , retinitis ( 9 ) and infection of other organs ( 9 ) . | NA | {
"id": "C0243026",
"name": "systemic infection",
"pos": [
24,
42
]
} | {
"id": "C0009450",
"name": "infection",
"pos": [
91,
100
]
} |
the activity of amoxycillin/clavulanic acid was compared with that of cefuroxime and cefotaxime , alone and combined with metronidazole , in preventing the development of infections in the mouse caused by subcutaneous injection of a beta-lactamase-producing strain , escherichia coli e96 , or a mixed inoculum of e. coli... | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
70,
80
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
85,
95
]
} |
amoxycillin/clavulanic acid , cefuroxime and cefotaxime were equally efficacious in preventing development of the e. coli e96 monoinfection at clinically achievable concentrations . | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
30,
40
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
45,
55
]
} |
however , the activity of cefuroxime against e. coli e96 in the mixed infection was markedly reduced as was , to a lesser extent , that of cefotaxime . | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
26,
36
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
139,
149
]
} |
co-administration of metronidazole improved slightly the activity of cefuroxime against e. coli e96 in the mixed infection , but had no such enhancing effect on cefotaxime . | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
69,
79
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
161,
171
]
} |
results of in-vitro studies showed that cefuroxime and cefotaxime were stable in a culture of e. coli e96 , and were rapidly bactericidal against this strain . | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
40,
50
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
55,
65
]
} |
these in-vitro data explain the findings of the in-vivo studies , and establish that the beta-lactamase activity of bact . fragilis vpi 8908 was responsible for the diminished activity of cefuroxime and cefotaxime , combined with metronidazole , against a mixed e . | NA | {
"id": "C0007562",
"name": "cefuroxime",
"pos": [
188,
198
]
} | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
203,
213
]
} |
two hundred and eighty-one strains of enterococci isolated from blood cultures of 281 consecutive patients during 1980-1988 and 108 strains of enterococci isolated from miscellaneous clinical materials during 1988 were studied for high-level resistance ( mic greater than 2000 mg/l ) to amikacin , kanamycin , streptomyc... | NA | {
"id": "C0002499",
"name": "amikacin",
"pos": [
287,
295
]
} | {
"id": "C0022487",
"name": "kanamycin",
"pos": [
298,
307
]
} |
before 1985 there was no enterococcal strain with high-level resistance to amikacin , gentamicin , tobramycin or netilmicin , but 14 % were resistant to kanamycin and 21 % to streptomycin . | NA | {
"id": "C0002499",
"name": "amikacin",
"pos": [
75,
83
]
} | {
"id": "C0022487",
"name": "kanamycin",
"pos": [
153,
162
]
} |
prevalence of high-level aminoglycoside resistance of enterococci isolated from miscellaneous sources during 1988 was higher ( amikacin , 5 % ; kanamycin , 37 % ; streptomycin , 31 % ; gentamicin , 24 % , tobramycin , 27 % ; netilmicin , 10 % ) . | NA | {
"id": "C0002499",
"name": "amikacin",
"pos": [
127,
135
]
} | {
"id": "C0022487",
"name": "kanamycin",
"pos": [
144,
153
]
} |
taken together with our previous finding that al3+ enhances the naf-induced ca2+ mobilization in mob 3-4 cells , these results suggest that f- combined with al3+ ( i.e. , alf4- ) is a more potent stimulator of pge2 synthesis in cells than f- alone , and that the alf4- -enhanced pge2 synthesis may be caused by an increa... | NA | {
"id": "C0037508",
"name": "naf",
"pos": [
64,
67
]
} | {
"id": "C0016327",
"name": "f",
"pos": [
400,
401
]
} |
comparison of the deduced amino acid sequences of sir-fp and nadph-cytochrome p-450 oxidoreductase ( porter , t. d. , and kasper , c.b . ( 1985 ) proc . natl . acad . sci . u. s.a. 82 , 973-977 ) also showed identities that suggest these two proteins are descended from a common precursor , which contained binding regio... | NA | {
"id": "C0016223",
"name": "sir-fp",
"pos": [
50,
56
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
242,
250
]
} |
both the thiolate and phosphate inhibition results are consistent with charge repulsion by an anionic group in the urease active site . | NA | {
"id": "C0031603",
"name": "phosphate",
"pos": [
22,
31
]
} | {
"id": "C0003075",
"name": "anionic",
"pos": [
94,
101
]
} |
in contrast to odc synthesized in vitro , that isolated from cells was not degraded by the reticulocyte lysate degradation system , suggesting that post-translational modifications may be involved in regulating odc degradation . | NA | {
"id": "C0029282",
"name": "odc",
"pos": [
15,
18
]
} | {
"id": "C0007054",
"name": "odc",
"pos": [
211,
214
]
} |
these results indicate that pabi is a novel protease which differs from any known proteases including cytosolic high molecular weight proteases . | NA | {
"id": "C0030946",
"name": "protease",
"pos": [
44,
52
]
} | {
"id": "C0030940",
"name": "proteases",
"pos": [
134,
143
]
} |
the lectin appeared homogeneous by gel electrophoresis at ph 4.3 and gave a single protein band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mr = 33,000-36,000 . | NA | {
"id": "C0023206",
"name": "lectin",
"pos": [
4,
10
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
83,
90
]
} |
a monoclonal antibody ( mab ) , designated 0.5 alpha , derived from a patient with adult t-cell leukemia was found previously to neutralize the human t-cell leukemia/lymphotropic type i ( htlv-i ) virus in in vitro assays and bind to the major envelope glycoprotein ( gp46 ) of htlv-i ( matsushita , s. , guroff , m.r. ,... | NA | {
"id": "C0023492",
"name": "adult t-cell leukemia",
"pos": [
83,
104
]
} | {
"id": "C0023493",
"name": "human t-cell",
"pos": [
144,
156
]
} |
the latter assay permitted the isolation of a 28-kda gtp-binding protein that bound [ alpha-32p ] gtp prominently but was only poorly detected with the gtp gamma s-binding assay . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
53,
56
]
} | {
"id": "C0079469",
"name": "gtp gamma",
"pos": [
152,
161
]
} |
site-directed mutagenesis was performed with the phosphate starvation-inducible outer membrane porin phoe of escherichia coli k-12 to study the molecular basis of its anion selectivity . | NA | {
"id": "C0031603",
"name": "phosphate",
"pos": [
49,
58
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
167,
172
]
} |
the stable gtp analogues , gtp-gamma-s ( guanosine 5'- ( gamma-thio ) triphosphate ) and gmp-pnp ( guanosine 5'- ( beta , gamma-imido ) triphosphate ) , potentiated calcium-evoked catecholamine release in a dose-dependent manner . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
11,
14
]
} | {
"id": "C0079469",
"name": "gtp-gamma-s",
"pos": [
27,
38
]
} |
the stable gtp analogues , gtp-gamma-s ( guanosine 5'- ( gamma-thio ) triphosphate ) and gmp-pnp ( guanosine 5'- ( beta , gamma-imido ) triphosphate ) , potentiated calcium-evoked catecholamine release in a dose-dependent manner . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
11,
14
]
} | {
"id": "C0018366",
"name": "gmp-pnp",
"pos": [
89,
96
]
} |
phage t7 dna polymerase contains escherichia coli thioredoxin as a subunit and is a 1:1 complex with t7 gene 5 protein . | NA | {
"id": "C0039938",
"name": "thioredoxin",
"pos": [
50,
61
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
111,
118
]
} |
in contrast to its synergistic effect with other known growth factors , the b subunit markedly inhibited dna synthesis induced by the phorbol ester , 12-o-tetradecanoyl-phorbol 13-acetate ( tpa ) . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
134,
147
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoyl-phorbol 13-acetate",
"pos": [
150,
187
]
} |
in extracts from the rat hypothalamus , approximately equimolar amounts of ang- ( 1-7 ) , ang-ii , and ang-i were detected ( 1.10 , 1.18 , and 1.45 pmol/g of tissue , respectively ) . | NA | {
"id": "C0003018",
"name": "ang",
"pos": [
75,
78
]
} | {
"id": "C0003009",
"name": "ang-ii",
"pos": [
90,
96
]
} |
in extracts from the rat hypothalamus , approximately equimolar amounts of ang- ( 1-7 ) , ang-ii , and ang-i were detected ( 1.10 , 1.18 , and 1.45 pmol/g of tissue , respectively ) . | NA | {
"id": "C0003018",
"name": "ang",
"pos": [
75,
78
]
} | {
"id": "C0003006",
"name": "ang-i",
"pos": [
103,
108
]
} |
levels of ang- ( 1-7 ) ( 0.19 pmol/g ) and ang-i ( 0.14 pmol/g ) were approximately 20 % that of ang-ii . | NA | {
"id": "C0003018",
"name": "ang",
"pos": [
10,
13
]
} | {
"id": "C0003006",
"name": "ang-i",
"pos": [
97,
102
]
} |
levels of ang- ( 1-7 ) ( 0.19 pmol/g ) and ang-i ( 0.14 pmol/g ) were approximately 20 % that of ang-ii . | NA | {
"id": "C0003018",
"name": "ang",
"pos": [
10,
13
]
} | {
"id": "C0003009",
"name": "ang-ii",
"pos": [
97,
103
]
} |
in plasma , the major angiotensin was ang-i ( 0.13 pmol/ml ) , with lower levels of ang- ( 1-7 ) and ang-ii ( 0.01-0.02 pmol/ml ) . | NA | {
"id": "C0003006",
"name": "ang-i",
"pos": [
38,
43
]
} | {
"id": "C0003018",
"name": "ang",
"pos": [
101,
104
]
} |
in plasma , the major angiotensin was ang-i ( 0.13 pmol/ml ) , with lower levels of ang- ( 1-7 ) and ang-ii ( 0.01-0.02 pmol/ml ) . | NA | {
"id": "C0003018",
"name": "ang",
"pos": [
22,
25
]
} | {
"id": "C0003009",
"name": "ang-ii",
"pos": [
101,
107
]
} |
the site in calcineurin , the ca2+/calmodulin ( cam ) -dependent protein phosphatase , which is phosphorylated by ca2+/cam-dependent protein kinase ii ( cam-kinase ii ) has been identified . | NA | {
"id": "C0248868",
"name": "cam-dependent protein kinase ii",
"pos": [
119,
150
]
} | {
"id": "C0006776",
"name": "cam-kinase",
"pos": [
153,
163
]
} |
we have demonstrated previously that cultured rat ovarian granulosa cells synthesize and secrete apoe , and this production of apoe is increased by agents that stimulate protein kinase a ( cyclic amp-dependent enzyme ) ( for example , cholera toxin ) and protein kinase c ( ca2+/phospholipid-dependent enzyme ) ( for exa... | NA | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
327,
363
]
} | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
368,
381
]
} |
other calcium-binding proteins , including calmodulin and troponin c , also possess this unique calcium-binding design , which is designated ef-hand or calmodulin fold . | NA | {
"id": "C0006732",
"name": "calcium-binding",
"pos": [
6,
21
]
} | {
"id": "C0077400",
"name": "troponin c",
"pos": [
58,
68
]
} |
however , cellular choline and phosphocholine levels were increased with a similar time course to that observed for diacylglycerol production , suggesting that ras increases phosphatidylcholine turnover but not phosphatidylinositol turnover . | NA | {
"id": "C0008405",
"name": "choline",
"pos": [
19,
26
]
} | {
"id": "C0031716",
"name": "phosphocholine",
"pos": [
31,
45
]
} |
down-regulation of protein kinase c ( by prolonged exposure to phorbol esters prior to scrape loading ) blocked the ability of ras protein to elevate the levels of diacylglycerol , choline , and phosphocholine . | NA | {
"id": "C0008405",
"name": "choline",
"pos": [
181,
188
]
} | {
"id": "C0031716",
"name": "phosphocholine",
"pos": [
195,
209
]
} |
in order to dissociate the effect of virus and endogenous interferon on the induction process , we examined the gene expression in vero cells , which have undergone homozygous deletion of type 1 interferon genes , and in vnpt-159 cells , which were derived from vero cells by insertion of an inducible human interferon b... | NA | {
"id": "C0021747",
"name": "interferon",
"pos": [
58,
68
]
} | {
"id": "C0021743",
"name": "type 1 interferon",
"pos": [
188,
205
]
} |
an in situ screening procedure is described that permits the detection of rna-dependent dna polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus . | NA | {
"id": "C0035379",
"name": "rna-dependent dna polymerase",
"pos": [
74,
102
]
} | {
"id": "C1955880",
"name": "reverse transcriptase",
"pos": [
149,
170
]
} |
amino-terminal gln and asn are tertiary destabilizing residues in that they are destabilizing through their ability to be converted , via selective deamidation , into secondary destabilizing residues glu and asp . | NA | {
"id": "C0017797",
"name": "gln",
"pos": [
15,
18
]
} | {
"id": "C0061472",
"name": "glu",
"pos": [
200,
203
]
} |
the third exon encodes a 79-amino acid sequence that includes the 15 amino acids immediately preceding an alternating serine-glycine 21-amino acid sequence for glycosaminoglycan attachment , and the subsequent c-terminal 43 amino acids ; | NA | {
"id": "C0002520",
"name": "amino acids",
"pos": [
69,
80
]
} | {
"id": "C0017890",
"name": "glycine",
"pos": [
125,
132
]
} |
the predicted alpha-helical region showed limited homology to alpha-helical regions of cytoskeletal proteins and certain other proteins , but no extensive homologies were found in the cytovillin cdna or the deduced amino acid sequence to other registered dna or protein sequences . | NA | {
"id": "C0010852",
"name": "cytoskeletal proteins",
"pos": [
87,
108
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
127,
135
]
} |
the effects of camp were assessed in chinese hamster fibroblast ( chw ) cells expressing either the wild type human beta 2ar receptor ( ch-beta 2 ) or mutated forms of the receptor lacking the consensus sequences for phosphorylation by the camp-dependent protein kinase . | NA | {
"id": "C0016030",
"name": "fibroblast",
"pos": [
53,
63
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
72,
77
]
} |
in vitro and in vivo , binding of des to dna has been found previously , but covalent dna adducts could not be identified . | NA | {
"id": "C0012854",
"name": "dna",
"pos": [
41,
44
]
} | {
"id": "C0242958",
"name": "dna adducts",
"pos": [
86,
97
]
} |
this set of dna adducts closely matched patterns generated in vitro by reaction of diethylstilbestrol-4',4 '' -quinone with dna or 2'-deoxyguanosine 3'-monophosphate . | NA | {
"id": "C0242958",
"name": "dna adducts",
"pos": [
12,
23
]
} | {
"id": "C0012854",
"name": "dna",
"pos": [
124,
127
]
} |
first , monovalent anions , glutamate , acetate , chloride , and thiocyante , follow the lyotropic series in their effects on both h1 histone displacement and chromatin aggregation . | NA | {
"id": "C0003075",
"name": "anions",
"pos": [
19,
25
]
} | {
"id": "C0008203",
"name": "chloride",
"pos": [
50,
58
]
} |
since another aldehyde , acetaldehyde , the first metabolite of ethanol , also stimulates collagen transcription in cultured fibroblasts ( brenner , d. a. , and chojkier , m. ( 1987 ) j. biol . chem 262 , 17690-17696 ) , we investigated whether ascorbic acid induces lipid peroxidation in cultured cells and if this is t... | NA | {
"id": "C0001992",
"name": "aldehyde",
"pos": [
14,
22
]
} | {
"id": "C0000966",
"name": "acetaldehyde",
"pos": [
25,
37
]
} |
the addition of malondialdehyde ( 200 microm ) , a product of lipid peroxidation , to cultured human fibroblasts also increased 2-3-fold collagen production and procollagen alpha 1 ( i ) mrna levels . | NA | {
"id": "C0009325",
"name": "collagen",
"pos": [
137,
145
]
} | {
"id": "C0033235",
"name": "procollagen",
"pos": [
161,
172
]
} |
relation of serum lipoproteins and lipids to the abo blood groups in patients with intermittent claudication . | NA | {
"id": "C0023820",
"name": "lipoproteins",
"pos": [
18,
30
]
} | {
"id": "C0023779",
"name": "lipids",
"pos": [
35,
41
]
} |
this enhanced responsiveness was specifically due to the reactivated viral k-ras protein rather than the temperature shift because the same temperature shift did not affect adenylate cyclase responsiveness in uninfected nrk cells , nor was it a result of the mitogenic stimulus since reacting the mitogenic pp60v-src pro... | NA | {
"id": "C0042736",
"name": "viral",
"pos": [
69,
74
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
317,
324
]
} |
the effects of heparin and other glycosaminoglycans ( gags ) on the mitogenicity and stability of acidic fibroblast growth factor ( afgf ) were studied . | NA | {
"id": "C0019134",
"name": "heparin",
"pos": [
15,
22
]
} | {
"id": "C0017973",
"name": "glycosaminoglycans",
"pos": [
33,
51
]
} |
several gags structurally related to heparin such as chondroitin-4-sulfate , chondroitin-6-sulfate , dermatan sulfate , and hyaluronic acid neither potentiated nor stabilized afgf mitogenic activity . | NA | {
"id": "C0017973",
"name": "gags",
"pos": [
8,
12
]
} | {
"id": "C0019134",
"name": "heparin",
"pos": [
37,
44
]
} |
cultures of fibroblasts in fibrin lattices : models for the study of metabolic activities of the cells in physiological conditions . | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
12,
23
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
97,
102
]
} |
when the fibrin lattices were prepared in silicone-coated glass petri dishes , fibroblasts induced retraction of lattices to an extent dependent on both cell density and serum concentration , the cells stopped dividing , and their protein and collagen syntheses proceeded at a lower rate , like that in collagen lattices... | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
79,
90
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
196,
201
]
} |
fluorescence microscopy has been used to study the cell surface distribution of the complement receptor for c3bi ( cr3 ) on human neutrophils during locomotion . | NA | {
"id": "C0034793",
"name": "complement receptor",
"pos": [
84,
103
]
} | {
"id": "C0079785",
"name": "cr3",
"pos": [
115,
118
]
} |
in particular , swiss 3t3 cells express 90,000 pdgf-aa binding sites ( a/b-receptors ) per cell , whereas human fibroblasts express only 20,000 a/b-receptors per cell . | NA | {
"id": "C0007634",
"name": "cell",
"pos": [
26,
30
]
} | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
112,
123
]
} |
confluent cultures of human endothelial cells deposit into extracellular matrix ( ecm ) distinct heparan sulfate proteoglycans ( hspg ) which modulate acidic fibroblast growth factor 's ( afgf ) ability to stimulate human endothelial cell mitogenic capacity . | NA | {
"id": "C0079349",
"name": "acidic",
"pos": [
151,
157
]
} | {
"id": "C0016026",
"name": "endothelial cell",
"pos": [
222,
238
]
} |
the role of protein kinase c activation in changes in muscarinic receptor functions and in the appearance of biochemical properties characteristic of neuronal cells was studied in sh-sy5y human neuroblastoma cells induced to differentiate with the phorbol ester 12-o-tetradecanoylphorbol-13-acetate ( tpa ) . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
248,
261
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
262,
298
]
} |
mixing nuclear protein extracts that contain mnda with nuclei from cells that do not express this protein demonstrated that the mnda redistributes from the freely soluble form to the nuclear residual fraction as a consequence of dnase i digestion . | NA | {
"id": "C0028589",
"name": "nuclear",
"pos": [
7,
14
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
98,
105
]
} |
exposure of chondrocytes , which had been metabolically labelled with 3h-acetate , to exogenous hyaluronate or to streptomyces hyaluronidase resulted in the release of 36-38 % of the endogenous , labelled chondroitin sulfate from the cell layer into the incubation solution . | NA | {
"id": "C0225369",
"name": "chondrocytes",
"pos": [
12,
24
]
} | {
"id": "C0007634",
"name": "cell",
"pos": [
234,
238
]
} |
finally , chondrocytes transfected with a plasmid carrying the cat gene driven by the collagen ii promoter/enhancer sequence displayed high levels of cat activity when cultured in control media , but treatment of the cells with a combination of the two growth factors resulted in a dramatic reduction of cat activity , i... | NA | {
"id": "C0225369",
"name": "chondrocytes",
"pos": [
10,
22
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
217,
222
]
} |
the catecholamine isoproterenol ( iso ) binds to beta-adrenergic cell surface receptors and thereby inhibits cell responses such as o2- production stimulated by formyl peptides . | NA | {
"id": "C0007412",
"name": "catecholamine",
"pos": [
4,
17
]
} | {
"id": "C0022245",
"name": "isoproterenol",
"pos": [
18,
31
]
} |
however , iso did not inhibit o2- generation activated by 1 microm ionophore a23187 , the protein kinase c activators phorbol ester ( pma , 100 ng/ml ) and oleoylacetylglycerol ( oag , 50 microm ) , and the g-protein activator naf ( 40 mm ) . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
118,
131
]
} | {
"id": "C0039654",
"name": "pma",
"pos": [
134,
137
]
} |
the phosphorylation of an mr 82,000 protein ( p82 ) in the triton x-100 extract of the particulate fraction of mouse epidermis is dependent on the phorbol ester 12-o-tetradecanoylphorbol-13-acetate ( tpa ) or diacylglycerol and phospholipid and , contrary to protein kinase c ( pkc ) -catalyzed phosphorylation , can not... | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
147,
160
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
161,
197
]
} |
the efficacy ( cip3 = ip3 = ( 4,5 ) ip2 much greater than ip4 ) and action of the various inositol phosphates in an intact cell and their inability to induce meiotic cell division are discussed . | NA | {
"id": "C0021549",
"name": "ip3",
"pos": [
16,
19
]
} | {
"id": "C0021554",
"name": "inositol phosphates",
"pos": [
90,
109
]
} |
in the differentiation sequence mf ii-mf iii-pmf iv - pmf v - pmf vi 14 cell-type-specific marker proteins have been found in the cytoplasmic and nuclear fraction , also 24 cell-type-specific marker proteins have been found in the membrane-bound protein fraction , and 11 cell-type-specific marker proteins in the secret... | NA | {
"id": "C0033684",
"name": "proteins",
"pos": [
98,
106
]
} | {
"id": "C0025252",
"name": "membrane",
"pos": [
231,
239
]
} |
in these studies , monolayer cultures were dissociated by trypsinization and no detectable difference was noted in the efficiency of cell dissociation between normal and dmd fibroblast cultures . | NA | {
"id": "C0007634",
"name": "cell",
"pos": [
133,
137
]
} | {
"id": "C0016030",
"name": "fibroblast",
"pos": [
174,
184
]
} |
there was also a marked increase in alanine/tyrosine , while minor or no changes occurred with other amino acids . | NA | {
"id": "C0001898",
"name": "alanine",
"pos": [
36,
43
]
} | {
"id": "C0002520",
"name": "amino acids",
"pos": [
101,
112
]
} |
urinary nag was found to correlate positively with albuminuria and proteinuria ( r = 0.95 and 0.93 , respectively ) , as well as with ribonuclease and lysozyme ( r = 0.93 and 0.60 ; p less than 0.01 ) in patients with persistent proteinuria . | NA | {
"id": "C0001925",
"name": "albuminuria",
"pos": [
51,
62
]
} | {
"id": "C0033687",
"name": "proteinuria",
"pos": [
229,
240
]
} |
the ion pairs were labelled with dansylhydrazine and the hydrazones were separated by hplc on a capcell-pak c8 ( silicone polymer-coated silica gel modified with octyl groups ) reversed-phase column using methanol-0.5 % ( w/v ) sodium acetate-50 % ( v/v ) acetic acid ( 57:42:1 , v/v/v ) as the mobile phase . | NA | {
"id": "C0142785",
"name": "sodium acetate",
"pos": [
228,
242
]
} | {
"id": "C0000983",
"name": "acetic",
"pos": [
256,
262
]
} |
by the combined use of these devices , noiseless uv tracing was successfully demonstrated in two model experiments using thermolabile two-phase solvent systems : separation of flavonoids from the sea buckthorn ethanol extract with chloroform-methanol-water ( 4:3:2 , v/v/v ) and separation of bacitracin components with ... | NA | {
"id": "C0001963",
"name": "methanol",
"pos": [
242,
250
]
} | {
"id": "C0001962",
"name": "ethanol",
"pos": [
331,
338
]
} |
silica beads have been coated with a polysaccharide ( dextran or agarose ) , substituted by a calculated amount of positively charged diethylaminoethyl functions in order to neutralize the negatively charged silanol groups of silica and to facilitate the formation of a hydrophilic polymeric layer on the inorganic surfa... | NA | {
"id": "C0032594",
"name": "polysaccharide",
"pos": [
37,
51
]
} | {
"id": "C0036681",
"name": "agarose",
"pos": [
65,
72
]
} |
a comparison of methods for the purification of naturally occurring mouse monoclonal autoantibodies , of the immunoglobulin m ( igm ) isotype , has been performed to determine the optimal strategies for the isolation of igm from ascites fluid and in vitro tissue culture hybridoma supernatants . | NA | {
"id": "C0021017",
"name": "immunoglobulin",
"pos": [
109,
123
]
} | {
"id": "C0020861",
"name": "igm",
"pos": [
220,
223
]
} |
with these latter separation techniques , monoclonal igm was eluted with a variety of other ascites fluid or supernatant proteins , including those with apparent molecular weights identical to those of mouse igg and albumin . | NA | {
"id": "C0033684",
"name": "proteins",
"pos": [
121,
129
]
} | {
"id": "C0001924",
"name": "albumin",
"pos": [
216,
223
]
} |
it was demonstrated on short columns packed with these anion exchangers that ( i ) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers ; | NA | {
"id": "C0021966",
"name": "i",
"pos": [
0,
1
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
210,
215
]
} |
proteins were eluted under gradient conditions with a range of alkali metal halides to vary systematically the anion and cation species in the series f- , cl- , and br- and li+ , na+ and k+ . | NA | {
"id": "C0003075",
"name": "anion",
"pos": [
111,
116
]
} | {
"id": "C0016327",
"name": "f",
"pos": [
150,
151
]
} |
protein zc values ( i.e . slopes of the ion-exchange retention plots , derived from the dependency of the logarithmic capacity factor log k ' on the concentration of the ionic displacer ) generally increased when both the anion and cation were either chaotropic , e.g . | NA | {
"id": "C0022023",
"name": "ionic",
"pos": [
170,
175
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
222,
227
]
} |
protein zc values ( i.e . slopes of the ion-exchange retention plots , derived from the dependency of the logarithmic capacity factor log k ' on the concentration of the ionic displacer ) generally increased when both the anion and cation were either chaotropic , e.g . | NA | {
"id": "C0022023",
"name": "ionic",
"pos": [
170,
175
]
} | {
"id": "C0007447",
"name": "cation",
"pos": [
232,
238
]
} |
conversely , zc values decreased when the displacer salt contained an anion-cation combination of a chaotropic and a kosmotropic ion , e.g . | NA | {
"id": "C0003075",
"name": "anion",
"pos": [
70,
75
]
} | {
"id": "C0022023",
"name": "ion",
"pos": [
129,
132
]
} |
conversely , zc values decreased when the displacer salt contained an anion-cation combination of a chaotropic and a kosmotropic ion , e.g . | NA | {
"id": "C0007447",
"name": "cation",
"pos": [
76,
82
]
} | {
"id": "C0022023",
"name": "ion",
"pos": [
129,
132
]
} |
all isoforms appeared to be high-molecular-weight proteins ( greater than 60 a ) when subsequently analyzed by high-performance size-exclusion chromatography . | NA | {
"id": "C0597298",
"name": "isoforms",
"pos": [
4,
12
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
50,
58
]
} |
when the protein is very hydrophobic and insoluble , as in the case of membrane proteins , up to 60 % aq . formic acid has been used . | NA | {
"id": "C0033684",
"name": "protein",
"pos": [
9,
16
]
} | {
"id": "C0025252",
"name": "membrane proteins",
"pos": [
71,
88
]
} |
in order to evaluate systematically the influence of the structures of the acyl group in the chiral thiol reagents , a series of novel n-acyl-l-cysteines was synthesized [ acyl = n-butyryl , isobutyryl ( i-but ) , pivaloyl , benzoyl ) and the chromatographic behaviour of the diastereomers formed was compared with those... | NA | {
"id": "C0001047",
"name": "n-acetyl-l-cysteine",
"pos": [
349,
368
]
} | {
"id": "C0010654",
"name": "l-cysteine",
"pos": [
397,
407
]
} |
the effects of ph , ionic strength , organic modifier , heptanesulphonic acid and citric acid content of a high-performance liquid chromatography mobile phase on the chromatographic and electrochemical behaviour of norepinephrine , epinephrine , dopamine , 3,4-dihydroxybenzylamine , 3,4-dihydroxyphenylethylene glycol ,... | NA | {
"id": "C0028351",
"name": "norepinephrine",
"pos": [
215,
229
]
} | {
"id": "C0014563",
"name": "epinephrine",
"pos": [
232,
243
]
} |
a high-performance liquid chromatographic method is described for the determination in human urine and plasma of catecholamines , their precursor and metabolites [ amino compounds ( norepinephrine , epinephrine , dopamine , normetanephrine , metanephrine , 3-methoxytyramine and l-dopa ) , acidic compounds ( 3,4-dihydro... | NA | {
"id": "C0028351",
"name": "norepinephrine",
"pos": [
182,
196
]
} | {
"id": "C0014563",
"name": "epinephrine",
"pos": [
199,
210
]
} |
a high-performance liquid chromatographic method is described for the determination in human urine and plasma of catecholamines , their precursor and metabolites [ amino compounds ( norepinephrine , epinephrine , dopamine , normetanephrine , metanephrine , 3-methoxytyramine and l-dopa ) , acidic compounds ( 3,4-dihydro... | NA | {
"id": "C0028351",
"name": "norepinephrine",
"pos": [
182,
196
]
} | {
"id": "C0028385",
"name": "normetanephrine",
"pos": [
224,
239
]
} |
a high-performance liquid chromatographic method is described for the determination in human urine and plasma of catecholamines , their precursor and metabolites [ amino compounds ( norepinephrine , epinephrine , dopamine , normetanephrine , metanephrine , 3-methoxytyramine and l-dopa ) , acidic compounds ( 3,4-dihydro... | NA | {
"id": "C0014563",
"name": "epinephrine",
"pos": [
185,
196
]
} | {
"id": "C0025562",
"name": "metanephrine",
"pos": [
242,
254
]
} |
this method was successfully used to assay human plasma samples from a pilot moricizine bioavailability study in which tablets and solution containing moricizine hydrochloride and [ 13c6 ] moricizine , respectively , were simultaneously administered . | NA | {
"id": "C0724636",
"name": "moricizine hydrochloride",
"pos": [
151,
175
]
} | {
"id": "C0079856",
"name": "moricizine",
"pos": [
189,
199
]
} |
in contrast to polyclonal antisera to clsv , which always contain some antibodies to host components , monoclonal antibodies are highly specific for the virus . | NA | {
"id": "C0020960",
"name": "antisera",
"pos": [
26,
34
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
114,
124
]
} |
in contrast to polyclonal antisera to clsv , which always contain some antibodies to host components , monoclonal antibodies are highly specific for the virus . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
71,
81
]
} | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
103,
124
]
} |
however , the phorbol ester 12-o-tetradecanoyl phorbol-13-acetate ( 100 nm ) had no consistent effect on mrna levels despite stimulating secretion in four of six cases . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
14,
27
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoyl phorbol-13-acetate",
"pos": [
28,
65
]
} |
in contrast , coincubation of granulosa luteal cells with fsh plus igf-i or cg plus igf-i dramatically enhanced e2 production at 4 and 6 days ( on the average , 4-fold ) , and the effects were sustained throughout the culture period . | NA | {
"id": "C0021665",
"name": "igf-i",
"pos": [
67,
72
]
} | {
"id": "C0037657",
"name": "igf",
"pos": [
84,
87
]
} |
delayed occurrence of an lh surge after hcg administration during ovarian stimulation with gonadotrophins : effect of lhrh treatment . | NA | {
"id": "C0018062",
"name": "hcg",
"pos": [
40,
43
]
} | {
"id": "C0018061",
"name": "gonadotrophins",
"pos": [
91,
105
]
} |
alpha il-1 was the most predominant form of il-1 found in the sera of all groups , and its level in hiv-seronegative intravenous drug abusers was elevated compared with the level in controls , whereas its levels in asymptomatic hiv-seropositive intravenous drug abusers and asymptomatic hiv-seronegative and hiv-seroposi... | NA | {
"id": "C0600251",
"name": "alpha il-1",
"pos": [
0,
10
]
} | {
"id": "C0021755",
"name": "il-1",
"pos": [
44,
48
]
} |
by using [ 35s ] methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody , iga antibody responses to large glycoprotein , fusion protein , nucleoprotein , phosphoprotein , and matrix protein were demonstrated in colostrum and milk . | NA | {
"id": "C0020960",
"name": "antiserum",
"pos": [
65,
74
]
} | {
"id": "C0003241",
"name": "antibody",
"pos": [
118,
126
]
} |
by using [ 35s ] methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody , iga antibody responses to large glycoprotein , fusion protein , nucleoprotein , phosphoprotein , and matrix protein were demonstrated in colostrum and milk . | NA | {
"id": "C0028612",
"name": "nucleoprotein",
"pos": [
178,
191
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
222,
229
]
} |
by using [ 35s ] methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody , iga antibody responses to large glycoprotein , fusion protein , nucleoprotein , phosphoprotein , and matrix protein were demonstrated in colostrum and milk . | NA | {
"id": "C0031689",
"name": "phosphoprotein",
"pos": [
194,
208
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
222,
229
]
} |
verotoxin ( vt ) , which is immunologically unrelated to vt1 ( shiga-like toxin i ) , was purified from the culture filtrate of escherichia coli hemorrhagic colitis serogroup o157 : h7 strain 3657 by copper ion chelate affinity chromatography followed by anion-exchange chromatography . | NA | {
"id": "C0022023",
"name": "ion",
"pos": [
207,
210
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
255,
260
]
} |
univariate analysis showed the following factors to be significantly associated with p. aeruginosa o16 bacteremia : the severity of granulocytopenia at the time of the bacteremia , more days with fever , the administration of ticarcillin or an aminoglycoside , the receipt of a greater number of antimicrobial agents for... | NA | {
"id": "C0002556",
"name": "aminoglycoside",
"pos": [
244,
258
]
} | {
"id": "C1136254",
"name": "antimicrobial agents",
"pos": [
296,
316
]
} |
all strains tested were susceptible or moderately susceptible to amikacin , azlocillin , cefotaxime , ceftazidime , ceftriaxone , chloramphenicol , gentamicin , mezlocillin , moxalactam , piperacillin , trimethoprim-sulfamethoxazole , sulfisoxazole , thienamycin , tobramycin , and trimethoprim . | NA | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
89,
99
]
} | {
"id": "C0007561",
"name": "ceftriaxone",
"pos": [
116,
127
]
} |
we showed that levels of hrv antibodies in serum specimens obtained before clinical onset of diarrhea varied widely , and , for most infants in the diarrheal group , levels of these antibodies were similar to those in the serum specimens obtained at the same times from the corresponding age- and birth weight-matched co... | NA | {
"id": "C0011991",
"name": "diarrhea",
"pos": [
93,
101
]
} | {
"id": "C0011992",
"name": "infants",
"pos": [
326,
333
]
} |
the rate of spontaneous abortion in this series was 12.9 % , similar to that observed in cases of threatened abortion without observable hematoma . | NA | {
"id": "C0000786",
"name": "spontaneous abortion",
"pos": [
12,
32
]
} | {
"id": "C0000821",
"name": "threatened abortion",
"pos": [
98,
117
]
} |
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