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human cellular fps/fes cdna rescued via retroviral shuttle vector encodes myeloid cell ncp92 and has transforming potential .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1708010", "name": "fps", "pos": [ 15, 18 ] }
we have generated a cdna copy of human c-fps/fes from a 13 kb genomic dna by means of a retroviral shuttle vector , and have begun characterization of its biological and biochemical properties .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 33, 38 ] }
{ "id": "C1708010", "name": "fps", "pos": [ 41, 44 ] }
neisseria gonorrhoeae produces two phenotypically distinct types of iga1 proteases , each of which cleaves a specific peptide bond in the hinge region of the human iga1 heavy chain .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 158, 163 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 169, 180 ] }
human sperm immaturity was tested by nuclear chromatin decondensation ( ncd ) in 1 % sodium dodecylsulphate ( sds ) of spermatozoa used in 74 consecutive ivf treatments .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 154, 157 ] }
the expression of ras oncogene product p21 in human malignant pleurisy and primary lung cancer was studied immunocyto-histochemically with monoclonal antibodies ( moabs ) rp-28 and rp-35 against ras p21 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 195, 198 ] }
we constructed fusion genes consisting of the mouse metallothionein i ( mt ) 5 ' region and the coding region of either the human h-ras gene ( c-rasp3 ) or a mutated allele ( c-rast24 ) ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 124, 129 ] }
{ "id": "C0079471", "name": "h-ras gene", "pos": [ 130, 140 ] }
its transcribed sequences were shown to be distributed over a 30-kb span within a molecularly cloned 45-kb segment of human dna which contained the transforming gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 118, 123 ] }
{ "id": "C0029016", "name": "transforming gene", "pos": [ 148, 165 ] }
thus , these antigenic determinants appear to be different from the classical hla class i antigens and could be the human counterpart of the murine qa system or of the second h-2k gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 116, 121 ] }
{ "id": "C1708995", "name": "second", "pos": [ 168, 174 ] }
two regions of very high homology were delineated by analyzing the sequence of this chicken er cdna and comparing it to the sequences of the human er , human glucocorticoid , and chicken progesterone receptors and the p75-erba fusion product of the avian erythroblastosis virus .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 141, 146 ] }
{ "id": "C0242987", "name": "erba", "pos": [ 222, 226 ] }
human dna from 11 individuals was analysed by southern blot for the immunoglobulin genes coding for the heavy chain variable region ( vh ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 104, 115 ] }
we present evidence that the human igf-ii gene has at least two promoters , and their expression may be developmentally controlled in the liver .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 29, 34 ] }
{ "id": "C1334091", "name": "igf-ii gene", "pos": [ 35, 46 ] }
expression in e. coli of erg : a novel gene in humans related to the ets oncogene .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 47, 53 ] }
{ "id": "C0812380", "name": "ets", "pos": [ 69, 72 ] }
these data indicate that ( 1 ) human and gp peripheral lung tissues respond to h1 , but not h2 , stimulation with the generation of pgf2 alpha and pge ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 31, 36 ] }
{ "id": "C0919509", "name": "h2", "pos": [ 92, 94 ] }
mild detergent treatment ( 0.1 % sarkosyl-0.1 % beta-mercaptoethanol ) of dane particle-rich fraction from human serum resulted in the release of core particles together with hbe antigen activity when examined by the reversed passive haemagglutination method .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 107, 112 ] }
{ "id": "C1415484", "name": "hbe", "pos": [ 175, 178 ] }
molecular analysis of normal and abnormal human globin genes and their gene products has recently provided information on the precise genetic events that result in hemoglobinopathies .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 42, 47 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 48, 60 ] }
in the human , the presence of fibrin in crescents , as well as the response to heparinization in some cases , support the role of coagulation in the genesis of extracapillary cell proliferation .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 7, 12 ] }
{ "id": "C1414672", "name": "genesis", "pos": [ 150, 157 ] }
in two of the independent hybrids that produced human heavy chains , human chromosome 14 was the only human chromosome present in the hybrid cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 48, 53 ] }
{ "id": "C1420192", "name": "heavy chains", "pos": [ 54, 66 ] }
an amphotropic retrovirus packaging cell line was constructed in which the gag , pol , and env genes of the helper virus are separated on two different plasmids and in which the packaging signals and 3 ' long terminal repeats are removed .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 15, 25 ] }
{ "id": "C0017360", "name": "pol", "pos": [ 81, 84 ] }
an amphotropic retrovirus packaging cell line was constructed in which the gag , pol , and env genes of the helper virus are separated on two different plasmids and in which the packaging signals and 3 ' long terminal repeats are removed .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 15, 25 ] }
{ "id": "C0017343", "name": "env genes", "pos": [ 91, 100 ] }
these antibodies were shown to block fertilization of human oocytes in in vitro fertilization ( ivf ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 54, 59 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 96, 99 ] }
we have previously reported highly potent basophil/mast cell ( bmc ) and basophil/eosinophil ( eo ) colony-stimulating activities ( csa ) in conditioned medium derived from cultured human nasal polyp epithelial scrapings ( np-cm ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 182, 187 ] }
{ "id": "C1706141", "name": "np", "pos": [ 223, 225 ] }
pulsed field gel electrophoresis and southern blot analysis showed that the human oncogenes , hst1 and int2 , which code for proteins homologous to fibroblast growth factors , are less than 45 kb apart on the long arm of chromosome 11 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 76, 81 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 82, 91 ] }
pulsed field gel electrophoresis and southern blot analysis showed that the human oncogenes , hst1 and int2 , which code for proteins homologous to fibroblast growth factors , are less than 45 kb apart on the long arm of chromosome 11 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 76, 81 ] }
{ "id": "C0812290", "name": "int2", "pos": [ 103, 107 ] }
hiv-1 recombinant retrovirus ( rrv ) , where the gag , pol and part of env genes have been exchanged for the reporter nlslacz gene , expresses the reporter gene only in presence of tat .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 18, 28 ] }
{ "id": "C0017360", "name": "pol", "pos": [ 55, 58 ] }
hiv-1 recombinant retrovirus ( rrv ) , where the gag , pol and part of env genes have been exchanged for the reporter nlslacz gene , expresses the reporter gene only in presence of tat .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 18, 28 ] }
{ "id": "C0017343", "name": "env genes", "pos": [ 71, 80 ] }
these results illustrate the proximity of the human vh , dh and jh segments involved in creation of the complete heavy chain genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 113, 124 ] }
human and bovine cervical mucus penetration tests ( n = 57 ) were performed preceding ivf to test their prognostic value as sperm function tests for ivf .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 149, 152 ] }
the aim of the present study was to identify and characterize the sites of pcp action ( sigma and/or pcp receptors ) in human peripheral blood leukocytes ( pbl ) using [ 3h ] haloperidol or 1,3 di ( 2- ( [ 5-3h ] tolyl ) guanidine ( [ 3h ] dtg ) to specifically label sigma receptors and 3,4- [ 3h ] - ( n ) - [ 1- ( 2-thienyl ) -cyclohexyl ] -piperidine ( [ 3h ] tcp ) to specifically label pcp receptors .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 120, 125 ] }
{ "id": "C3538902", "name": "di", "pos": [ 350, 352 ] }
our data suggest that adenosine-induced suppression of histamine 's positive chronotropic and inotropic effects on human myocardium results both from an a1-mediated attenuation of h2-stimulatory effects and from the uncovering of h1 negative chronotropic and inotropic effects .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 115, 120 ] }
{ "id": "C0919509", "name": "h2", "pos": [ 180, 182 ] }
this region of the genome has been implicated in chronic lymphocytic leukaemia and also encodes the int-2 human oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 106, 111 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 112, 120 ] }
in a survey of primary human bladder carcinomas from 24 patients , using the nih/3t3 transfection nude mouse tumor assay , we have detected an activated c-h-ras-1 gene in four cases .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 23, 28 ] }
{ "id": "C1705572", "name": "h-ras", "pos": [ 155, 160 ] }
the human c-ets-1 proto-oncogene locus was shown to translocate from chromosome 11 to 9 or 4 in certain patients with acute monocytic leukemia .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C0812201", "name": "ets-1", "pos": [ 12, 17 ] }
the protein encoded in a novel human oncogene isolated by transfection of kaposi 's sarcoma dna is a growth factor with significant homology to basic and acidic fgfs .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 31, 36 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 37, 45 ] }
neurotransmitter modulation of the human class ii gene dr alpha on multiforme glioblastoma cell lines .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 35, 40 ] }
{ "id": "C0017349", "name": "class ii gene", "pos": [ 41, 54 ] }
antibody p7 was used to quantitate the amount of p-glycoprotein present in drug-resistant kb lines at various levels of resistance and to demonstrate the presence of p-glycoprotein in nih 3t3 cells transfected with a cloned mdr1 cdna or human genomic dna encoding mdr1 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 237, 242 ] }
{ "id": "C0376622", "name": "mdr1", "pos": [ 264, 268 ] }
several recent discoveries indicate that the ras genes , frequently activated to a transforming potential in some human tumours , belong to a large family that can be divided into three main branches : the first branch represented by the ras , ral and rap genes ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 114, 119 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 238, 241 ] }
several recent discoveries indicate that the ras genes , frequently activated to a transforming potential in some human tumours , belong to a large family that can be divided into three main branches : the first branch represented by the ras , ral and rap genes ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 114, 119 ] }
{ "id": "C0812284", "name": "ral", "pos": [ 244, 247 ] }
human retinal pigment epithelial cells differentially express mhc class ii ( hla , dp , dr and dq ) antigens in response to in vitro stimulation with lymphokine or purified ifn-gamma .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C0017349", "name": "mhc class ii", "pos": [ 62, 74 ] }
the human tgf-beta 3 gene is spread over seven exons as in the case of the tgf-beta 1 gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C1336622", "name": "tgf-beta 3", "pos": [ 10, 20 ] }
the human tgf-beta 3 gene is spread over seven exons as in the case of the tgf-beta 1 gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C1366557", "name": "tgf-beta 1 gene", "pos": [ 75, 90 ] }
using peptide antibodies which had been raised against sequences predicted to be specific for the human c-fyn gene product , the c-fyn protein was identified .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 98, 103 ] }
{ "id": "C0812303", "name": "fyn", "pos": [ 131, 134 ] }
in human iggl the two heavy chains are crosslinked in the central portion of the molecule by two disulfide bridges forming a double chain bis-cystinyl cyclic peptide in parallel alignment .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 3, 8 ] }
{ "id": "C1420192", "name": "heavy chains", "pos": [ 22, 34 ] }
in the course of these studies it was determined that nt2/d1 ec cells also express several transcripts that hybridize with a cdna probe for the human pdgf a-chain .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 144, 149 ] }
{ "id": "C1335200", "name": "pdgf", "pos": [ 150, 154 ] }
the influence of maternal dietary zinc intake and recombinant human interleukin-1 alpha ( rhil-1 alpha ) administration on metallothionein gene expression and the distribution of 65zn were investigated .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 62, 67 ] }
{ "id": "C1708430", "name": "interleukin-1 alpha", "pos": [ 68, 87 ] }
in human erythroleukemia ( k562 ) cells , in which embryonic and fetal globin genes are transcribed , all of the sequences we examined from the beta-globin domain replicated early during s phase , while in hela cells , in which globin genes are transcriptionally silent , these sequences replicated late during s .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 3, 8 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 228, 240 ] }
however , some regions of the genome showed some homology to retroviral gag and pol open reading frames .
organism_has_gene
{ "id": "C0035366", "name": "retroviral", "pos": [ 61, 71 ] }
{ "id": "C0017360", "name": "pol", "pos": [ 80, 83 ] }
when the mutant ras2val-19 , which carries the equivalent of the human h-rasval-12 oncogene , was grown under conditions in which ras1 expression is repressed , the camp signal was absent .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 65, 70 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 83, 91 ] }
genomic sequences for the large subunit of human rna polymerase ii corresponding to a part of the fifth exon were inserted into an expression vector at the carboxy-terminal end of the beta-galactosidase gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 43, 48 ] }
{ "id": "C1705846", "name": "at", "pos": [ 149, 151 ] }
to evaluate the possible role of this protease in the transformed phenotype , we transfected cloned genes for mouse or human mep into mouse nih 3t3 cells with an expression vector for the dominant , selectable human multidrug resistance ( mdr1 ) gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 119, 124 ] }
{ "id": "C0376623", "name": "multidrug", "pos": [ 216, 225 ] }
using a human tgf beta 1 cdna probe , we have detected two distinct tgf beta rnas in cultured primary chick embryo chondrocytes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C1366557", "name": "tgf beta 1", "pos": [ 14, 24 ] }
the predicted protein includes a signal peptide of 20-23 amino acids as in human tgf beta 1 and 2 , and a precursor protein consisting of 412 amino acids , which can be cleaved at a lys-arg site to produce a 112 amino acid processed peptide containing nine cysteine residues in the same positions as in human tgf beta 1 and 2 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 75, 80 ] }
{ "id": "C1366557", "name": "tgf beta 1", "pos": [ 309, 319 ] }
at the nucleotide level , the processed coding region of tgf beta 3 shows 72 % and 76 % identity with the processed coding regions of human tgf beta 1 and tgf beta 2 , respectively ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 134, 139 ] }
{ "id": "C1366557", "name": "tgf beta 1", "pos": [ 140, 150 ] }
in human dna , sequences homologous to this element were located at the 5 ' end of the c-myb gene , i.e . in the same position as in the chicken and mouse genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 3, 8 ] }
{ "id": "C1704837", "name": "end", "pos": [ 76, 79 ] }
a hindiii polymorphism identified by the human early growth response gene 2 ( egr2 ) on chromosome 10 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 41, 46 ] }
{ "id": "C1414315", "name": "early growth response", "pos": [ 47, 68 ] }
the treatment of human infertility has changed enormously since the introduction of ivf and gift .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 17, 22 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 84, 87 ] }
however , the abnormality was not so marked in the murine as in the human trisomy , and the ( n-3 ) / ( n-6 ) ratio in epg was primarily elevated on account of decreased proportions of 20:4 ( n-6 ) and 22:4 ( n-6 ) , there being no significant increase in ( n-3 ) pufa .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 68, 73 ] }
{ "id": "C1825057", "name": "epg", "pos": [ 119, 122 ] }
using a human transforming growth factor beta 1 ( tgf beta ) cdna probe , we have detected an rna species migrating at about 1.7 kilobases in cultured primary chicken embryo chondrocytes that is distinct from chicken tgf beta 1 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C1366557", "name": "tgf beta", "pos": [ 217, 225 ] }
1 ) the binding characteristics of [ 3h ] 5-ht ( 5-hydroxytryptamine , serotonin ) were investigated in membrane preparations of several regions from calf , pig and human brain in the presence of 100 nmol/l 8-oh-dpat ( 8-hydroxy-2 [ di-n-dipropylamino ] tetralin ) and 100 nmol/l mesulergine in order to mask 5-ht1a and 5-ht1c sites .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 165, 170 ] }
{ "id": "C3538902", "name": "di", "pos": [ 238, 240 ] }
rearrangement and loss of genetic material in dna of human ductal breast carcinoma was analyzed by restriction enzyme digestion , agarose gel electrophoresis and southern blot hybridization using 32p labeled c-ha-ras-1 and multilocus , minisatellite 15.1.11.4 as a probe .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 53, 58 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 213, 216 ] }
the cells of the reactivated colony produced human ha-ras 1 mrna and p21 , and their dna displayed transforming activity for nih/3t3 recipient cells that was due to the original human ha-ras 1 oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 45, 50 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 187, 190 ] }
the cells of the reactivated colony produced human ha-ras 1 mrna and p21 , and their dna displayed transforming activity for nih/3t3 recipient cells that was due to the original human ha-ras 1 oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 45, 50 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 193, 201 ] }
we conclude that the methylation status of the promoter region of the human ha-ras 1 modulates the expression and consequently the transforming activity of the oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 70, 75 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 79, 82 ] }
we conclude that the methylation status of the promoter region of the human ha-ras 1 modulates the expression and consequently the transforming activity of the oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 70, 75 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 160, 168 ] }
after incubating human serum with [ 125i ] ioxynil , 2diep against anti-whole human serum and rocket immunoelectrophoresis against anti-tbpa , albumin or tbg were carried out .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 17, 22 ] }
{ "id": "C3814864", "name": "tbg", "pos": [ 154, 157 ] }
mouse ifn inhibited the development of transformed foci in nih 3t3 cultures transfected with the viral ha-musv ( ras ) and mo-musv ( mos ) oncogenes , or with the human bladder carcinoma ras ej/t24 dna .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 163, 168 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 187, 190 ] }
analysis of total feline dna by genomic blot hybridization , using the viral oncogene of abelson murine leukemia virus as a specific probe , has led to the identification of multiple v-abl homologous genetic sequences in the cat genome .
organism_has_gene
{ "id": "C0000742", "name": "abelson murine leukemia virus", "pos": [ 89, 118 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 183, 188 ] }
these studies demonstrate that in human umbilical arteries 11-hete , 15-hete and a presumed di-hete are the products of cyclooxygenase .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 34, 39 ] }
{ "id": "C3538902", "name": "di", "pos": [ 92, 94 ] }
we have isolated cloned human c-abl probes to analyze the organization and expression of abl genes in patients with cml and in k562 cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 24, 29 ] }
{ "id": "C1704928", "name": "abl", "pos": [ 89, 92 ] }
the reduction in hpgrf-induced gh release remained significant when corrected for the reduced intracellular gh content in the hypothyroid cultures .
organism_has_gene
{ "id": "C0086418", "name": "hpgrf", "pos": [ 17, 22 ] }
{ "id": "C1418759", "name": "hpgrf", "pos": [ 17, 22 ] }
the human cellular myc gene is one of about 20 cellular oncogenes which code for a variety of proteins including protein kinases and growth factors .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C0026902", "name": "myc gene", "pos": [ 19, 27 ] }
southern blot hybridization analysis of dna from human-chinese hamster somatic cell hybrids showed that the human igf-ii gene is located on chromosome 11 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 49, 54 ] }
{ "id": "C1334091", "name": "igf-ii gene", "pos": [ 114, 125 ] }
using the same igf-ii variant probe , a cosmid was isolated which contains the human igf-ii gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 79, 84 ] }
{ "id": "C1334091", "name": "igf-ii gene", "pos": [ 85, 96 ] }
the transfectants were examined for the presence of human oncogenes in relation to tumorigenic potential and composition of surface-located fucosyl glycopeptides by gel filtration , concanavalin-a-binding and high-performance liquid chromatography .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 52, 57 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 58, 67 ] }
the data suggest that functions provided by human ras-oncogenes contribute to the alteration of membrane glycopeptides on tumor cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 44, 49 ] }
{ "id": "C0034678", "name": "ras-oncogenes", "pos": [ 50, 63 ] }
binding of 12.7g3 , as detected by immunofluorescence using flow microfluorometry , was positive for two human cell lines , gm 3161 and hfb-1 , both expressing hla-dr2 , and negative for two other cell lines , gm 3104 ( dr1,1 ) and gm 3164 ( dr4,4 ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 105, 110 ] }
{ "id": "C1333247", "name": "dr1", "pos": [ 220, 223 ] }
a segment of the coding sequence of the abelson murine leukemia virus transforming gene ( v-abl ) has been inserted into a plasmid vector that allows its efficient and regulated expression in escherichia coli .
organism_has_gene
{ "id": "C0000742", "name": "abelson murine leukemia virus", "pos": [ 40, 69 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 90, 95 ] }
purified human peripheral blood polymorphonuclear neutrophils ( pmn ) were incubated at 37 degrees c with the fluorescent membrane potential sensitive cyanine dye di-o-c ( 5 ) ( 3 ) and exposed to a number of stimulatory agents ( n-formylmethionylleucylphenylalanine ( fmlp ) , cytochalasin b ( cyto b ) + fmlp , phorbol myristate acetate ( pma ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 9, 14 ] }
{ "id": "C3538902", "name": "di", "pos": [ 163, 165 ] }
the single protein encoded by abelson murine leukemia virus is a fusion of sequence from the retroviral gag genes with the v-abl sequence .
organism_has_gene
{ "id": "C0000742", "name": "abelson murine leukemia virus", "pos": [ 30, 59 ] }
{ "id": "C0080279", "name": "genes", "pos": [ 108, 113 ] }
our results show that interferon severely inhibits , to a similar extent , the stable transformation of ltk- and nih 3t3 cells by the chicken thymidine kinase ( tk ) gene , ecogpt gene , simian virus 40 , v-ha-ras , and human c-ha-ras and c-ki-ras oncogenes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 220, 225 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 244, 247 ] }
tissue-specific levels of human glucose-6-phosphate dehydrogenase correlate with methylation of specific sites at the 3 ' end of the gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 26, 31 ] }
{ "id": "C1705846", "name": "at", "pos": [ 111, 113 ] }
since the human hras-2 also resides on the x chromosome , it appears that the cellular hras-2 gene ( or pseudogene ) conserved its chromosomal localization during mammalian evolution .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 10, 15 ] }
{ "id": "C0079471", "name": "hras", "pos": [ 87, 91 ] }
conjugated bilirubins in the biles of other animals ( human , monkey , pony , cat , rat and dog ) are chemically more diverse and include mono- , di- and mixed disconjugates of glucuronic acid , xylose and glucose in proportions that give distinct patterns for each species .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 54, 59 ] }
{ "id": "C3538902", "name": "di", "pos": [ 239, 241 ] }
tyrosine-specific protein kinase ( atp : protein phosphotransferase , ec 2.7.1.37 ) activity was measured in normal human nonadherent peripheral blood lymphocytes using synthetic peptide substrates having sequence homologies with either pp60src or c-myc .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 116, 121 ] }
{ "id": "C0086661", "name": "c-myc", "pos": [ 248, 253 ] }
we have examined a panel of human lung cancer cell lines for amplification and expression of the c-myc , n-myc , and c-myb oncogenes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 28, 33 ] }
{ "id": "C0812287", "name": "n-myc", "pos": [ 105, 110 ] }
human ferritins purified from normal liver ( lf ) and from hepatocellular carcinoma ( hf ) showed different biochemical and immunological characteristics .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1416933", "name": "lf", "pos": [ 45, 47 ] }
a target structure for a series of human cloned natural killer cell lines is recognized by both anti-tnktar and 4f2 monoclonal antibodies .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 35, 40 ] }
{ "id": "C1420192", "name": "4f2", "pos": [ 112, 115 ] }
nih/3t3 cells transfected with human tumor dna containing activated ras oncogenes express the metastatic phenotype in nude mice .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 31, 36 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 68, 71 ] }
thus , transfection with human tumor dna containing activated ras oncogenes can induce the complete metastatic phenotype in nih/3t3 cells by a mechanism apparently unrelated to immune cell killing .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 25, 30 ] }
{ "id": "C0034678", "name": "ras oncogenes", "pos": [ 62, 75 ] }
we have cotransfected nmumg cells with psv2neo and genomic dna from the human ej bladder carcinoma line , a cell line which contains an activated c-rash oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 72, 77 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 153, 161 ] }
genomic dna transfection into a line of mouse epithelial cells , in combination with the selection of stable transfectants and tumor induction in nude mice , can be used to screen human tumor dna for the presence of activated oncogenes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 180, 185 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 226, 235 ] }
our results indicate that the activated human ras oncogene may confer metastatic potential onto lymphoid tumor cells , probably due to the induction of invasiveness .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 40, 45 ] }
{ "id": "C0034678", "name": "ras oncogene", "pos": [ 46, 58 ] }
the human heavy chain enhancer element was ligated tot he chimeric heavy and light chain genes , and this enhancer appeared to be obligatory for the efficient production of the chimeric antibody molecules .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 10, 21 ] }
monophasic action potential mapping in human subjects with normal electrocardiograms : direct evidence for the genesis of the t wave .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 39, 44 ] }
{ "id": "C1414672", "name": "genesis", "pos": [ 111, 118 ] }
we have examined the expression of the transformed phenotype in a series of clonal lines of nih/3t3 cells transfected with the human c-ha-rasval 12 oncogene and the neomycin phosphotransferase gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 127, 132 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 148, 156 ] }
one of these ligands , 125i-labeled pta-oh , a txa2/pgh2 receptor antagonist , has been extensively used to characterize the human platelet txa2/pgh2-binding site .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 125, 130 ] }
{ "id": "C0919509", "name": "pgh2", "pos": [ 145, 149 ] }
hemoglobin m iwate is caused by a c -- -- t transition in codon 87 of the human alpha 1-globin gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 74, 79 ] }
{ "id": "C2697529", "name": "alpha 1-globin gene", "pos": [ 80, 99 ] }
the serologic reactivity could be blocked by turkey sera to human b2-microglobulin but not by turkey anti-ia-like .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 60, 65 ] }
{ "id": "C1415587", "name": "b2-microglobulin", "pos": [ 66, 82 ] }
southern hybridization analyses with different portions of a full-length cdna indicate that the human mdr1 gene encompasses at least 70 kilobases of dna amplified in all highly multidrug-resistant cell lines .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 96, 101 ] }
{ "id": "C0376623", "name": "mdr1 gene", "pos": [ 102, 111 ] }
human placental villous trophoblast is known to be unreactive with w6/32 and other monoclonal antibodies recognizing monomorphic determinants of human class i mhc heavy chains , whereas extravillous cytotrophoblast in the placental bed is w6/32-reactive by immunohistology .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C0017355", "name": "class", "pos": [ 151, 156 ] }
human placental villous trophoblast is known to be unreactive with w6/32 and other monoclonal antibodies recognizing monomorphic determinants of human class i mhc heavy chains , whereas extravillous cytotrophoblast in the placental bed is w6/32-reactive by immunohistology .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1420192", "name": "heavy chains", "pos": [ 163, 175 ] }