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introduction of a human beta 2-microglobulin gene into l cells transfected with the hla-b27 gene rescued the expression of hla-b27 at the cell surface , as evidenced by reactivity with w6/32 , surface staining , and the presence of sialic acid on the heavy chain .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 18, 23 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 251, 262 ] }
in this study , we demonstrate that the murine polymorphic class ii antigens of an interspecies mouse-human hybrid , which has segregated the murine invariant chain gene , associates with the human invariant chain gene intracytoplasmically .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 102, 107 ] }
{ "id": "C3273406", "name": "invariant chain gene", "pos": [ 198, 218 ] }
in this study , we present evidence that functionally mature , normal human t cells have rearranged their t gamma genes but display a limited range of gene rearrangement choices .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 70, 75 ] }
{ "id": "C1420562", "name": "t", "pos": [ 168, 169 ] }
the protein product of this oncogene , p48v-myb , is partially encoded by the retroviral gag and env genes .
organism_has_gene
{ "id": "C0035366", "name": "retroviral", "pos": [ 78, 88 ] }
{ "id": "C0017343", "name": "env genes", "pos": [ 97, 106 ] }
to assess the relationship of hydralazine to human breast cancer , 3,419 women with breast cancer and 3,219 hospital control subjects were studied .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 45, 50 ] }
{ "id": "C1332438", "name": "3", "pos": [ 102, 103 ] }
comparison of the rat and human c-ha-ras -1 exons showed an area of poor homology immediately upstream of the human cap sites , followed further upstream by a region of close homology ( approximately equal to 60 base pairs ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 26, 31 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 37, 40 ] }
cultured human embryonal rhabdomyosarcoma cells express the basic fibroblast growth factor ( bfgf ) gene and they produce bfgf , which is apparently composed of two microheterogenous forms with mrs of 16,500 and 17,200 , respectively .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 9, 14 ] }
{ "id": "C1708015", "name": "basic fibroblast", "pos": [ 60, 76 ] }
on the basis of this experiment grade 2 water was used in the tygerberg hospital human ivf programme with good results .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 81, 86 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 87, 90 ] }
mitogenic stimulation of quiescent human fibroblasts ( hswp ) with a growth factor mixture ( consisting of epidermal growth factor ( egf ) , insulin , bradykinin , and vasopressin ) rapidly induces an increase in na influx via a ca-mediated activation of an amiloride-sensitive na/h exchanger .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 35, 40 ] }
{ "id": "C1420229", "name": "ca", "pos": [ 229, 231 ] }
purification and characterization of human colony-stimulating factor 1 from human pancreatic carcinoma ( mia paca-2 ) cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 37, 42 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 105, 108 ] }
colony-stimulating factor 1 ( csf-1 ) was purified from the serum-free conditioned medium of a human pancreatic carcinoma cell line ( mia paca-2 ) by a combination of conventional chromatography and high-performance liquid chromatography .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 95, 100 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 134, 137 ] }
using these radioimmunoassays and ras p21 purified from escherichia coli containing the full-length t24 mutant human harvey ras gene protein product as a standard , we have defined the actual amount of ras p21 per micrograms of total cellular protein , or per cell , in various ras transformed and 'normal ' mammalian cell lines .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 111, 116 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 278, 281 ] }
a panel of alloindifferent monoclonal antibodies ( mab 's ) was used in complement-dependent lysis to characterize human myeloid , erythroid and multipotential progenitors ( cfu-gm , bfu-e , cfu-gemm ) for their expression of mhc class ii hla-dr , -dp , and -dq products .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 115, 120 ] }
{ "id": "C0017349", "name": "mhc", "pos": [ 226, 229 ] }
to study the class ii genes of the major histocompatibility region of the sheep genome , human hla class ii genes corresponding to the known subregions in man ( dr , dq , dp , do , and dz ) were used for southern hybridization analysis of sheep dna and to probe a sheep genomic library .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 89, 94 ] }
{ "id": "C0017349", "name": "hla class ii genes", "pos": [ 95, 113 ] }
using the human c-erbb-2 cdna as a probe , we applied in situ hybridization techniques to acute promyelocytic leukemia cells with a 15 ; 17 chromosome translocation , t ( 15 ; 17 ) ( q ; 22 ; q21 ) , and were able to localize the c-erbb-2 gene on chromosome 17 to band q12 or q21 and on the proximal side of the breakpoint in the translocation .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 10, 15 ] }
{ "id": "C0243036", "name": "erbb", "pos": [ 232, 236 ] }
the results show that both human ril-1 alpha and ril-1 beta induce the differentiation of 70z/3 , but a higher concentration of ril-1 beta compared to ril-1 alpha is needed for a maximal response .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 27, 32 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 151, 154 ] }
these results show that human fetal astrocytes can be induced to express hla-dr by ifn-gamma in vitro and support the concept that astrocytes may function as antigen-presenting cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 24, 29 ] }
{ "id": "C1413237", "name": "hla-dr", "pos": [ 73, 79 ] }
various concentrations of recombinant human il 1 beta ( ril 1 ) and media conditioned by monocytes ( mcm ) , endothelial cells ( ecm ) , and endothelial cells cultured for 3 days in 50 % mcm ( ecmm ) or ril 1 ( ecmril 1 ) were added to marrow mononuclear cells cultured in methylcellulose .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 38, 43 ] }
{ "id": "C1709402", "name": "mcm", "pos": [ 187, 190 ] }
various concentrations of recombinant human il 1 beta ( ril 1 ) and media conditioned by monocytes ( mcm ) , endothelial cells ( ecm ) , and endothelial cells cultured for 3 days in 50 % mcm ( ecmm ) or ril 1 ( ecmril 1 ) were added to marrow mononuclear cells cultured in methylcellulose .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 38, 43 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 214, 217 ] }
preincubation with specific antisera to native human il 1 or ril 1 reduced by 75 to 100 % the activity of mcm in stimulating endothelial cell release of bpa , gm-csa , meg-csa , and mixed-cell csa .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 47, 52 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 61, 64 ] }
preincubation with specific antisera to native human il 1 or ril 1 reduced by 75 to 100 % the activity of mcm in stimulating endothelial cell release of bpa , gm-csa , meg-csa , and mixed-cell csa .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 47, 52 ] }
{ "id": "C1709402", "name": "mcm", "pos": [ 106, 109 ] }
these ab neutralized human recombinant il 1 alpha ( ril 1 alpha ) activity effectively , but did not interfere with human ril 1 beta , murine ril 1 alpha , or human ril 2 activity .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 21, 26 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 165, 168 ] }
the nucleotide sequence of the abl segment in the hz2-fesv genome was determined and compared with the murine and human v-abl and c-abl sequences .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 114, 119 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 120, 125 ] }
the 5 ' 189 base pairs of fv-abl correspond to 5 ' c-abl sequences not contained in abelson murine leukemia virus ( mulv ) v-abl .
organism_has_gene
{ "id": "C0000742", "name": "abelson murine leukemia virus", "pos": [ 84, 113 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 123, 128 ] }
comparison of the predicted amino acid sequence of fv-abl with those of abelson mulv v-abl and c-abl revealed five aa differences .
organism_has_gene
{ "id": "C0000742", "name": "abelson mulv", "pos": [ 72, 84 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 85, 90 ] }
a monoclonal antibody ( moab , sk-930 ) of the igg2a subclass to human pancreatic carcinoma cells ( mia-paca 2 ) was obtained by hybridization of spleen cells from immunized balb/c mice with murine myeloma cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 65, 70 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 100, 103 ] }
the complete coding sequence of the human a-raf-1 oncogene and transforming activity of a human a-raf carrying retrovirus .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 36, 41 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 50, 58 ] }
functionally , human a-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 15, 20 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 98, 101 ] }
an insert of 149 bp present at the 5 ' end of exon 7 of human c-sis but absent in the simian sarcoma virus v-sis oncogene was also present in the feline c-sis proto-oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 56, 61 ] }
{ "id": "C0037207", "name": "v-sis", "pos": [ 107, 112 ] }
the coordinated appearance of these groups of polypeptides and their similar size and reactivity with human sera and rabbit antisera produced against the isolated p52 as well as with a monoclonal antibody ( r3 ) suggested that most of these polypeptides are derived from post-translational modifications of one or a few initially synthesized polypeptides , possibly p52 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 102, 107 ] }
{ "id": "C1415346", "name": "p52", "pos": [ 366, 369 ] }
a scheme is proposed for generating the intact val-448-phe-545 polypeptide of human plasminogen which contains the fifth kringle domain of the plasmin heavy chain .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 78, 83 ] }
{ "id": "C1420192", "name": "heavy", "pos": [ 151, 156 ] }
[ 3h ] pk 11195 bound in a similar fashion to slide-mounted sections of human brain , thus allowing quantitative studies of the regional distribution of peripheral-type benzodiazepine binding sites to be made .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 72, 77 ] }
{ "id": "C1823521", "name": "peripheral", "pos": [ 153, 163 ] }
the results provide additional evidence that h2 receptors are present in the human cutaneous microcirculation , and add support to the clinical observation of therapeutic efficacy of h1 plus h2 blockers seen in some patients with chronic urticaria .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 77, 82 ] }
{ "id": "C0919509", "name": "h2", "pos": [ 191, 193 ] }
investigations on the regulation of human globin gene expression are assisted by a knowledge of the chromosomal location of the globin genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 36, 41 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 128, 140 ] }
selected compounds were compared with 1a in an avoidance-response acquisition model which differentiates between 1a and the human hallucinogens dom ( 2a ) and doet ( 2b ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 124, 129 ] }
{ "id": "C3539821", "name": "dom", "pos": [ 144, 147 ] }
at-125 , ( alpha s , 5s ) -alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid , at a concentration of 5 microm was found to inhibit the growth of human pancreatic carcinoma cells ( mia paca-2 ) by 78 % after 72 hours in continuous culture .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 151, 156 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 186, 189 ] }
ossification of some normal ligaments of the human skull which produce new structures : the pterygospinous and pterygoalar bars and foramina , and the caroticoclinoid foramen .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 45, 50 ] }
{ "id": "C1707156", "name": "bars", "pos": [ 123, 127 ] }
hemoglobin synthesis in somatic cell hybrids : coexpression of mouse with human or chinese hamster globin genes in interspecific somatic cell hybrids of mouse erythroleukemia cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 74, 79 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 99, 111 ] }
rna extracted from the human marrow x erythroleukemia hybrid annealed equally to both human and mouse globin complementary dna , indicating that coexpression of the globin genes of each species had occurred in the hybrid cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 23, 28 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 165, 177 ] }
papain solubilization of rat ag-b histocompatibility antigens produces ag-b molecules of about 59,000 daltons which have been shown to contain two fragments bound noncovalently : one fragment about 37,000 daltons carrying ag-b allospecificity , and another about 11,000 daltons , an apparent rat homologue of human beta2-microglobulin .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 309, 314 ] }
{ "id": "C1417683", "name": "beta2", "pos": [ 315, 320 ] }
the electrophoretic pattern of human fibroblast extracts displays three bands ( cathode to anode ) of hexosaminidase : hex b , hex a , hex c in normal strains , only one b band in tay-sachs strains , and only one c band in sandhoff strains .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 31, 36 ] }
{ "id": "C1415537", "name": "hex", "pos": [ 135, 138 ] }
the analysis of man-rodent hybrids ( hamster and mouse with normal and sandhoff human fibroblasts ) indicates a probable synteny between mpi , hex c , `` '' hex a fast '' '' , and `` '' hex a-like '' '' .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 80, 85 ] }
{ "id": "C1415537", "name": "hex", "pos": [ 186, 189 ] }
the b-cell mitogens which were used were dextran sulphate , lipopolysaccharide , purified protein derivative , as well as rabbit anti-human beta2-microglobulin serum .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 134, 139 ] }
{ "id": "C1417683", "name": "beta2", "pos": [ 140, 145 ] }
fifty-nine human dna samples derived from either normal tissues or hematopoietic neoplasias were examined for rearrangements in the mlvi-2 locus , a putative oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 11, 16 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 158, 166 ] }
this fragment , jy150/c5 , hybridized with two genomic bands in dna from human hla homozygotes -- presumably the hla-b locus gene and a closely related gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 73, 78 ] }
{ "id": "C1415561", "name": "hla-b locus gene", "pos": [ 113, 129 ] }
treatment of nih 3t3 cells with interferon ( ifn ) after transfection with human bladder carcinoma ej/t24 c-ha-ras1 oncogene dna caused inhibition of ras-induced cell transformation .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 75, 80 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 116, 124 ] }
treatment of nih 3t3 cells with interferon ( ifn ) after transfection with human bladder carcinoma ej/t24 c-ha-ras1 oncogene dna caused inhibition of ras-induced cell transformation .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 75, 80 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 150, 153 ] }
neither human nor sheep cells showed any enhanced [ 32p ] phosphate labelling of phosphoproteins when treated with 1-mono-oleoyl-rac-glycerol .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C1419227", "name": "1", "pos": [ 115, 116 ] }
isolation and partial characterization of genomic clones coding for a human pro-alpha 1 ( ii ) collagen chain and demonstration of restriction fragment length polymorphism at the 3 ' end of the gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 70, 75 ] }
{ "id": "C1705846", "name": "at", "pos": [ 172, 174 ] }
serum depleted of the first component of complement ( c1d ) was prepared by treating fresh human serum with sepharose-igg in the presence of triethylenetetramine-n , n , n ' , n '' , n '' ' , n '' '-hexa-acetic acid and di-isopropyl fluorophosphate at acidic ph ( 5.2 ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 91, 96 ] }
{ "id": "C3538902", "name": "di", "pos": [ 255, 257 ] }
the molecular weight difference in apoe observed among new and old world monkeys , as well as the molecular weight and/or charge differences observed among monkey , ape , and human apoe are consistent with structural changes in the apoe gene which have occurred following the divergence of the different species .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 175, 180 ] }
{ "id": "C1412481", "name": "apoe gene", "pos": [ 232, 241 ] }
like the corresponding human zeta 1 and zeta 2 genes , the first and second introns of the chimpanzee zeta 1 gene are occupied largely by tandem repeats of short oligonucleotides .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 23, 28 ] }
{ "id": "C1708995", "name": "second", "pos": [ 69, 75 ] }
our data suggest that the antigen associated with a human acute lymphocytic leukemia oncogene is ubiquitous , distributed among both neoplastic and normal hemopoietic cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 52, 57 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 85, 93 ] }
human plasma retinol-binding protein has been purified to homogeneity by a simple method that requires an ammonium sulfate fractionation , a hydrophobic interaction chromatography on phenyl-sepharose , which dissociates the complex between retinol-binding protein and its carrier , transthyretin , and a gel filtration on sephadex g-50 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1419320", "name": "plasma retinol-binding protein", "pos": [ 6, 36 ] }
the sequence of the 3 ' part of this cdna reveals a domainal organization of the derived protein similar to that of the known p-glycoproteins and an 80 % amino acid homology with the product of the human mdr1 gene ( chen et al. , 1986 ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 198, 203 ] }
{ "id": "C0376622", "name": "mdr1", "pos": [ 204, 208 ] }
human ( hela ) cells contain a protein , mltf , which specifically binds to a dna sequence in the adenovirus 2 major late promoter and activates transcription of that promoter .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1421376", "name": "mltf", "pos": [ 41, 45 ] }
combined protein and dna sequencing studies reveal that human rfs utilize a small number of light chain variable region genes , together with several heavy chain variable regions genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 56, 61 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 150, 161 ] }
three different methods were used for freezing human excess oocytes ( 320 frozen , 205 thawed ) in our ivf programme and the results of these methods were compared .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 47, 52 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 103, 106 ] }
however , the high degree of conservation of the phosphorylation sites for protein kinase c between human and mouse abl proteins suggests an important functional role .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 100, 105 ] }
{ "id": "C1704928", "name": "abl", "pos": [ 116, 119 ] }
we have examined the secretion of il-3 like activity from recipient normal mouse , rat and chinese or syrian hamster cells and their derivative cell lines obtained after transfection with recombinant plasmids carrying an exogenous human ras or myc gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 231, 236 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 237, 240 ] }
we have examined the secretion of il-3 like activity from recipient normal mouse , rat and chinese or syrian hamster cells and their derivative cell lines obtained after transfection with recombinant plasmids carrying an exogenous human ras or myc gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 231, 236 ] }
{ "id": "C0026902", "name": "myc gene", "pos": [ 244, 252 ] }
radioiodination of synthetic human 37-49 osteocalcin requires optimal labeling conditions in order to obtain a maximum of mono- and di-iodinated tracer with little contamination by tri- and tetra-iodinated products or `` '' radio-damage . '' ''
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 29, 34 ] }
{ "id": "C3538902", "name": "di", "pos": [ 226, 228 ] }
recombinant human interleukin-l ( ril-1 ) alpha and beta , which have 26 % homology in their amino acid sequence , stimulated arachidonic acid metabolism by squirrel monkey smooth muscle cells and rat liver cells ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 12, 17 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 34, 37 ] }
whereas human spermatozoa contain predominantly di- , tri- and tetraenoic fatty acids with up to 32 carbon atoms , boar , ram and bull spermatozoa also contain pentaenoic and/or hexaenoic acids with up to 34 carbon atoms .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C3538902", "name": "di", "pos": [ 48, 50 ] }
the regularity of the association between the translocation events and the tumors where they occur , together with the similarities between the human , mouse and rat systems can be interpreted by postulating that the activation of c-myc by the translocation represents an essential step in the genesis of these tumors .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 144, 149 ] }
{ "id": "C1414672", "name": "genesis", "pos": [ 294, 301 ] }
thus , the introduction of a single copy of a human chromosome 11 is sufficient to completely suppress the tumorigenic phenotype of hela cells and is suggestive of the presence of tumor-suppressor gene ( s ) on this chromosome .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C0079427", "name": "tumor-suppressor gene", "pos": [ 180, 201 ] }
the ia antigen-associated invariant chain ( in ) exists in humans as four related polypeptides , p33 , p35 , p41 and p43 , all associated with hla-class ii antigens .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 59, 65 ] }
{ "id": "C1704794", "name": "p35", "pos": [ 103, 106 ] }
chemical modification of a plasmid containing the human c-ha-ras proto-oncogene ( psvmbras-gpt ) in vitro with the ultimate carcinogens n-acetoxy-2-amino-6-methyldipyrido [ 1,2-a : 3',2'-d ] imidazole ( n-oac-glu-p-1 ) and n-acetoxy-4-aminoquinoline n-oxide ( n-oac-4aqo ) generated an activated oncogene that transformed nih3t3 cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 50, 55 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 296, 304 ] }
expression in escherichia coli of synthetic human interleukin-1 alpha genes encoding the processed active protein , mutant proteins , and beta-galactosidase fusion proteins .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 44, 49 ] }
{ "id": "C1708430", "name": "interleukin-1 alpha genes", "pos": [ 50, 75 ] }
to analyze human tumors for the presence of mutated ras oncogenes , a procedure was developed based on selective hybridization of mutation-specific oligodeoxynucleotide probes to genomic dna [ bos et al. , nucl . acids res . 12 ( 1984 ) 9155-9163 ] .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 11, 16 ] }
{ "id": "C0034678", "name": "ras oncogenes", "pos": [ 52, 65 ] }
rh 123 is recommended as an alternative means of assessing human spermatozoal viability , and the results arising from the use of this technique here are discussed with their particular relevance to semen freezing and preparation in ivf centers .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 59, 64 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 233, 236 ] }
normally , human adults express five types of immunoglobulin -- igm , igd , igg , ige and iga ( defined by the class of heavy chain constant region ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 11, 16 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 120, 131 ] }
the transforming gene found in a human bladder carcinoma cell line , t24 , is h-ras-1 , the human homologue of the harvey sarcoma virus oncogene ( v-h-ras ) 6-9 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 33, 38 ] }
{ "id": "C0079471", "name": "harvey sarcoma virus oncogene", "pos": [ 115, 144 ] }
the transforming gene found in a human bladder carcinoma cell line , t24 , is h-ras-1 , the human homologue of the harvey sarcoma virus oncogene ( v-h-ras ) 6-9 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 33, 38 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 151, 154 ] }
this bladder carcinoma oncogene has been shown to be of human origin , less than six kilobase pairs ( kbp ) in size , and closely related to the onc genes ( v-bas and v-ras ) of balb and harvey murine sarcoma viruses .
organism_has_gene
{ "id": "C0086418", "name": "human origin", "pos": [ 56, 68 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 169, 172 ] }
with reference , namely to bateson , bourdieu , lewin , pankow , he defines operational concepts which put into connection human communication , sociological context and genesis of personality .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 123, 128 ] }
{ "id": "C1414672", "name": "genesis", "pos": [ 170, 177 ] }
the cross-reacting antigen possessing tissue specificity was found in human tumor cells histogenetically associated with the tissues carrying the antigen ( basal cell carcinoma , squamous cell carcinoma of the skin , larynx , cervix ) but not in the cells of tumors of different genesis ( infiltrative mammary gland carcinoma , adenocarcinomas of the stomach and intestines ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 70, 75 ] }
{ "id": "C1414672", "name": "genesis", "pos": [ 279, 286 ] }
an antibody ( w6/32 ) which only reacts with the hla/beta 2-microglobulin complex in humans and not with the free hla heavy chain has the reverse specificity in some owl monkeys .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 85, 91 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 118, 129 ] }
somatostatin-immunoreactive cells ( gif-cells ) in the human and canine intestine had no direct cellular contacts with gli-immunoreactive cells ( gli-cells ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 55, 60 ] }
{ "id": "C0812304", "name": "gli", "pos": [ 146, 149 ] }
in contrast a limited number of gif-cells was found in the human duodenum where a few gli-cells were distributed and a few gif-cells were seen in the human ileum where a large number of gli-cells were located .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 59, 64 ] }
{ "id": "C0812304", "name": "gli", "pos": [ 186, 189 ] }
restriction enzyme digestion analysis and direct human globin gene cloning have permitted analysis of the physical arrangement of nucleotide sequences within and surrounding the human globin genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 49, 54 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 184, 196 ] }
the resting transmembrane potential ( em ) of human granulocytes was estimated using the fluorescent lipophilic cation , di-s-c3 ( 5 ) , from the null point for potassium -- i.e. , the external k concentration at which there is no change in em in response to valinomycin ( a k ionophore ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C3538902", "name": "di", "pos": [ 121, 123 ] }
rias developed with these antisera are effective for each hnp in the range of 5-320 pg .
organism_has_gene
{ "id": "C0086418", "name": "hnp", "pos": [ 58, 61 ] }
{ "id": "C1706141", "name": "hnp", "pos": [ 58, 61 ] }
in healthy , sitting , normally hydrated subjects of both sexes , vp-hnp and ot-hnp were , respectively , 73 +/- 5 and 382 +/- 30 pg/ml ( mean +/- sem ; n = 20 ) ;
organism_has_gene
{ "id": "C0086418", "name": "hnp", "pos": [ 69, 72 ] }
{ "id": "C1706141", "name": "hnp", "pos": [ 80, 83 ] }
women on oral contraceptives had elevated ( > 3 times normal ) levels of ot-hnp but normal levels of vp-hnp .
organism_has_gene
{ "id": "C0086418", "name": "hnp", "pos": [ 76, 79 ] }
{ "id": "C1706141", "name": "hnp", "pos": [ 104, 107 ] }
eleven patients who had the syndrome of inappropriate secretion of antidiuretic hormone had elevated levels of vp-hnp but not necessarily of ot-hnp .
organism_has_gene
{ "id": "C0086418", "name": "hnp", "pos": [ 114, 117 ] }
{ "id": "C1706141", "name": "hnp", "pos": [ 144, 147 ] }
in two of six subjects smoking caused a dramatic release of vp-hnp , as indexed by plasma levels which rose to more than 50 times the control values .
organism_has_gene
{ "id": "C0086418", "name": "hnp", "pos": [ 63, 66 ] }
{ "id": "C1706141", "name": "hnp", "pos": [ 63, 66 ] }
double-stranded dna copies of the rna gene coding for the haemagglutinin glycoproteins from human h2 and h3 pandemic strains of influenza virus have been cloned .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 92, 97 ] }
{ "id": "C0919509", "name": "h2", "pos": [ 98, 100 ] }
site-specific mutagenesis of the human interleukin-1 beta gene : the role of arginine residue at the n-terminal region .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 33, 38 ] }
{ "id": "C1708431", "name": "interleukin-1 beta gene", "pos": [ 39, 62 ] }
regulation of human estrogen receptor gene , epidermal growth factor receptor gene , and oncogenes by estrogen and antiestrogen in mcf-7 breast cancer cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 14, 19 ] }
{ "id": "C1414461", "name": "estrogen receptor gene", "pos": [ 20, 42 ] }
regulation of human estrogen receptor gene , epidermal growth factor receptor gene , and oncogenes by estrogen and antiestrogen in mcf-7 breast cancer cells .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 14, 19 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 89, 98 ] }
experiments using mcf-7 human breast cancer cells were carried out to study the regulatory effect of estrogen and antiestrogen on rna activities of oncogenes , estrogen receptor gene , and epidermal growth factor ( egf ) receptor gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 24, 29 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 148, 157 ] }
experiments using mcf-7 human breast cancer cells were carried out to study the regulatory effect of estrogen and antiestrogen on rna activities of oncogenes , estrogen receptor gene , and epidermal growth factor ( egf ) receptor gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 24, 29 ] }
{ "id": "C1414461", "name": "estrogen receptor gene", "pos": [ 160, 182 ] }
the transcript is derived from a single gene called nvhbox 1 , the newt homologue of xihbox 1 ( xenopus ) , hho.c8 ( human ) and hox-6.1 ( mouse ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 117, 122 ] }
{ "id": "C1415652", "name": "hox", "pos": [ 129, 132 ] }
hybrid proteins expressed by the lambda gt11 cdna library were blotted onto nitrocellulose filters , and incubated with three different radio-labeled dna probes containing the human epidermal growth factor ( egf ) receptor enhancer or the human c-erbb-2 promoter .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 176, 181 ] }
{ "id": "C0243036", "name": "erbb", "pos": [ 247, 251 ] }
the dbpa and dbpb-coded proteins also had an affinity for other cdna probes such as the human c-ski gene , but not for poly ( di-dc ) .poly ( di-dc ) , suggesting that the sequence ( s ) recognized by the dbpa and dbpb-coded proteins may occur frequently , or that these proteins bind to dna non-specifically in a different manner from that of histones .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 88, 93 ] }
{ "id": "C3538902", "name": "di", "pos": [ 314, 316 ] }
rabbit polyclonal antibodies ( pab ) were raised against a yeast ras-related protein yp20 and shown to be immunoreactive with human normal as well as altered ha-ras and ki-ras p21 gene products using immunoblotting and immunoprecipitation techniques .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 126, 131 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 172, 175 ] }
the genome possesses characteristic retrovirus features including structural genes , flanked by long terminal repeats , in the order gag , pol , and env and , in addition , four unique nonstructural genes , several of which appear to be essential in regulating virus replication .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 36, 46 ] }
{ "id": "C0017360", "name": "pol", "pos": [ 139, 142 ] }
the genome possesses characteristic retrovirus features including structural genes , flanked by long terminal repeats , in the order gag , pol , and env and , in addition , four unique nonstructural genes , several of which appear to be essential in regulating virus replication .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 36, 46 ] }
{ "id": "C0017343", "name": "env", "pos": [ 149, 152 ] }
by use of a probe derived from the cloned hst gene , it was shown that additional two transformants ( st7- and st15-derived ) also harboured human hst .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 141, 146 ] }
{ "id": "C0919466", "name": "hst", "pos": [ 147, 150 ] }