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the protein-tyrosine kinase activity and hybrid structure of p210bcr/abl are similar to the oncogene product of the abelson murine leukemia virus , p160gag/v-abl , which induces acute lymphomas .
organism_has_gene
{ "id": "C0000742", "name": "abelson murine leukemia virus", "pos": [ 116, 145 ] }
{ "id": "C0080279", "name": "v-abl", "pos": [ 156, 161 ] }
our results demonstrate that human c1s complement cleaves the heavy chain of human class i antigens into at least two fragments , with apparent molecular weights of 22,000 and 24,000 g/mol on sodium dodecyl sulphate-polyacrylamide gel electrophoresis ( sds-page ) , under both reducing and non-reducing conditions .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 29, 34 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 62, 73 ] }
multidrug resistance phenotype of human bro melanoma cells transfected with a wild-type human mdr1 complementary dna .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 34, 39 ] }
{ "id": "C0376622", "name": "mdr1", "pos": [ 94, 98 ] }
we have transfected a eukaryotic expression vector containing a mdr1 complementary dna isolated from normal human liver into human bro melanoma cells to study the drug-resistant phenotype produced by the exclusive overexpression of normal human mdr1 p-glycoprotein .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 108, 113 ] }
{ "id": "C0376622", "name": "mdr1", "pos": [ 245, 249 ] }
the studies have also revealed a new class of human oncogenes , the tumor suppressor genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 52, 61 ] }
the studies have also revealed a new class of human oncogenes , the tumor suppressor genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C0079427", "name": "tumor suppressor genes", "pos": [ 68, 90 ] }
this inhibitory effect of the e1a proteins is also observed with the ap-1 factor-binding enhansons from the polyomavirus and human metallothionein enhancers , and the mhc class i gene h-2kb enhanson , which binds the kbf1/h2tf1/tc-iib protein .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 125, 130 ] }
{ "id": "C0017355", "name": "mhc class i gene", "pos": [ 167, 183 ] }
this inhibitory effect of the e1a proteins is also observed with the ap-1 factor-binding enhansons from the polyomavirus and human metallothionein enhancers , and the mhc class i gene h-2kb enhanson , which binds the kbf1/h2tf1/tc-iib protein .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 125, 130 ] }
{ "id": "C1417708", "name": "kbf1", "pos": [ 217, 221 ] }
homologs for five human chromosome 11 and 15 loci ( calc , fes , hras-1 , igflr , tyr ) were localized within an 18-cm span telomeric to lhb .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 18, 23 ] }
{ "id": "C0079471", "name": "hras", "pos": [ 65, 69 ] }
human rtnf alpha augments anti-bacterial resistance in mice : potentiation of its effects by recombinant human ril-1 alpha .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 111, 114 ] }
these cells appear to be of human epithelial origin and express high levels of ha-ras transcript .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 28, 33 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 82, 85 ] }
we now show that purified human ril-6 , when administered alone at relatively high doses that are comparable to therapeutic levels of il-2 , mediated substantial reductions in the number of pulmonary and hepatic micrometastases from four distinct syngeneic tumors .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 26, 31 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 32, 35 ] }
high-resolution mapping of topol cleavages in the regions of human dna including the oncogene c-ha-ras and p53 , has revealed three kinds of topol cleavage sites : cleavage sites not affected by camptothecin ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 61, 66 ] }
{ "id": "C0034678", "name": "oncogene", "pos": [ 85, 93 ] }
we examined whether human and mouse spermatozoa release paf during in vitro incubation and assessed the effect of exogenous paf and the paf receptor antagonist web 2086 , a thieno-triazolodiazepine , on mouse in vitro fertilization ( ivf ) rate .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 20, 25 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 234, 237 ] }
oncogenic activation of the proto-oncogene c-abl in human leukemias occurs as a result of the addition of exons from the gene bcr and truncation of the first abl exon .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 52, 57 ] }
{ "id": "C1704928", "name": "abl", "pos": [ 158, 161 ] }
a cdna coding for human 5-amino-levulinate dehydratase was placed in a yeast expression vector under the control of the gal10 promoter .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 18, 23 ] }
{ "id": "C1707136", "name": "gal10", "pos": [ 120, 125 ] }
these data indicate molecular heterogeneity of the human glyr at the level of alpha subunit genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 51, 56 ] }
{ "id": "C1705846", "name": "at", "pos": [ 62, 64 ] }
however , not all strains are pathogenic for humans : virulence of strain c may be dependent on its ability both to colonize the intestine and to produce a toxin such as nag-st .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 45, 51 ] }
{ "id": "C1709111", "name": "nag", "pos": [ 170, 173 ] }
unfractionated human blood mononuclear cells ( mnc ) from normal and atopic donors cultured in enriched iscove 's modified dulbecco 's medium supplemented with 10 % fcs responded similarly to stimulation with purified human ril-4 ( rhil-4 ) with respect to the concentration required to induce ige synthesis and the magnitude and kinetics of the ige response .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 15, 20 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 224, 227 ] }
these findings indicate that when normal human t cells are stimulated in vitro in a manner that approximates a physiologic interaction with ag in vivo , ril-4 provides a potent inhibitory signal to il-2 responsive cells that is likely mediated by il-4-induced inhibition of il-2r expression .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 41, 46 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 153, 156 ] }
human dermal fibroblasts in culture secrete three protein-like neutrophil chemotactic factors , when stimulated either with human ril-1 alpha or il-1 beta ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1335621", "name": "ril", "pos": [ 130, 133 ] }
to examine whether a variant human oestrogen receptor gene , which differs from the wild-type gene in the b region , was associated with spontaneous abortion , obstetric histories of breast cancer patients with this variant were compared with those of breast cancer patients with the wild-type gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 29, 34 ] }
{ "id": "C1414461", "name": "oestrogen receptor gene", "pos": [ 35, 58 ] }
we examined frozen tissue sections of 50 invasive human breast carcinomas , including 45 ductal , four lobular , and one mucinous tumours , by immunocytochemical methods to determine the in situ distributions of c-erbb-2 , egfr , and pdgfr-beta-subunit .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 50, 55 ] }
{ "id": "C0243036", "name": "erbb", "pos": [ 214, 218 ] }
the philadelphia chromosome , widely implicated in human leukaemia , is the result of a reciprocal translocation t ( 9 ; 22 ) ( q34 ; q11 ) in which the abl oncogene located at 9q34 is translocated to chromosome 22q11 , where it is fused head-to-tail with 5 ' exons of the bcr gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 51, 56 ] }
{ "id": "C0080279", "name": "abl oncogene", "pos": [ 153, 165 ] }
we obtained human fra-1 cdna clones that can code for a protein of 271 amino acid residues with a calculated molecular weight of 29,413 and showed 90 % similarity with rat fra-1 protein .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 12, 17 ] }
{ "id": "C0812299", "name": "fra-1", "pos": [ 172, 177 ] }
characterization of human and mouse c-fes cdna clones and identification of the 5 ' end of the gene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 20, 25 ] }
{ "id": "C1704837", "name": "end", "pos": [ 84, 87 ] }
by low stringency screening of a xenopus laevis oocyte cdna library with a probe prepared from the yes oncogene of the avian retrovirus y73 , we have isolated clones encoding a 537 amino acid protein with 96 % amino acid identity to the protein product of the human fyn proto-oncogene .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 260, 265 ] }
{ "id": "C0812303", "name": "fyn", "pos": [ 266, 269 ] }
in the present study , we present the sequence for the human c-abl 3 ' untranslated region ( 3'utr ) and show that while human and murine c-abl cdna sequences are generally homologous , human c-abl transcripts unlike the murine c-abl transcripts do not differ significantly in their 3 ' utrs .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 55, 60 ] }
{ "id": "C1704928", "name": "abl", "pos": [ 230, 233 ] }
one parent is a synthetically altered harvey murine sarcoma virus rna that lacks a retroviral 3 ' terminus but carries a moloney murine retrovirus-derived envelope gene ( env ) fragment 3 ' of its transforming ras gene .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 136, 146 ] }
{ "id": "C0017343", "name": "env", "pos": [ 171, 174 ] }
further , these analyses identify two syntenic clusters of muscle-associated genes on the short arm of human chromosome 11 , one in the region of rhabdomyosarcoma locus that includes igf2 and th and the second the tightly linked myod1 and ldha loci , which have been evolutionarily conserved in homologous regions of both the mouse and the rat genomes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 103, 108 ] }
{ "id": "C1417574", "name": "myod1", "pos": [ 229, 234 ] }
a dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human bmp-2a resulted in cartilage by day 7 and bone formation by day 14 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 164, 169 ] }
{ "id": "C1332424", "name": "bmp-2a", "pos": [ 170, 176 ] }
production of il-5 messenger rna by transformed b-cell lines was verified by northern analysis using a 3.2-kilobase cloned dna fragment containing the full-length human il-5 gene , and immunoreactive il-5 was detected in b-cell culture supernatants .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 163, 168 ] }
{ "id": "C1708436", "name": "il-5 gene", "pos": [ 169, 178 ] }
suppression of synthesis of specific tropomyosin ( tm ) isoforms occurs commonly in human , murine , and avian fibroblasts transformed by retroviral oncogenes or other modalities .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 84, 89 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 149, 158 ] }
in this study we have asked whether defects in tm expression had relevance to human neoplasia , which arises most often in cells of the epithelial lineage rather than in fibroblasts and often is unrelated to demonstrable expression of oncogenes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 78, 83 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 235, 244 ] }
forty-five primary human lung carcinomas were evaluated for the loss of heterozygosity for genes on the short end of chromosome 11 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 19, 24 ] }
{ "id": "C1704837", "name": "genes", "pos": [ 91, 96 ] }
cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc , n-myc , epidermal growth factor receptor , and gli genes by southern blot analysis .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 50, 55 ] }
{ "id": "C0812287", "name": "n-myc", "pos": [ 121, 126 ] }
cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc , n-myc , epidermal growth factor receptor , and gli genes by southern blot analysis .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 50, 55 ] }
{ "id": "C1706548", "name": "gli genes", "pos": [ 168, 177 ] }
epstein-barr virus ( ebv ) -specific human cytotoxic t lymphocyte ( ctl ) lines from a virus-immune hla-a11+ donor cmc produced significant lysis of transfected mouse p815 target cells ( h-2d background ) expressing an introduced hla-a11 heavy chain gene and the ebv gene encoding latent membrane protein ( lmp ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 37, 42 ] }
{ "id": "C1420192", "name": "heavy", "pos": [ 238, 243 ] }
human ( hla-b7 ) and mouse ( h-2dp ) hybrid class i genes , encoding the leader , alpha 1 , and alpha 2 sequences of one species fused to the alpha 3 , transmembrane , and cytoplasmic domains of the other , were transfected into t2 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C0017355", "name": "class i genes", "pos": [ 44, 57 ] }
the human pthrp gene appears to use multiple promoters and contains alternatively spliced 3 ' exons which give rise to three pthrp mrna classes , each bearing multiple copies of an au motif that has been associated with mrna instability .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C1335272", "name": "pthrp gene", "pos": [ 10, 20 ] }
we show that one of these clones , clone d , codes for a 5-hydroxytryptamine1a ( 5-ht1a ) binding site since : 1 ) it possesses an intronless open reading frame encoding a protein with seven putative transmembrane domains and 89 % amino acid identity with the human 5-ht1a receptor ( g21 ) ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 260, 265 ] }
{ "id": "C3810555", "name": "g21", "pos": [ 284, 287 ] }
human neutrophils exposed to protein-coated polystyrene or cultured endothelial monolayers produce large quantities of h2o2 in response to soluble stimuli that elicit little or no secretion of reactive oxygen species from cells in suspension .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1423498", "name": "protein-coated", "pos": [ 29, 43 ] }
these data indicate that most types of human cells are resistant to the e3 19-kda glycoprotein effect , suggesting that virus-specific ctl recognition and lysis of most ad2/5-infected human cells should not be limited by e3 19-kda-mediated reduction in class i mhc ag expression .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 39, 44 ] }
{ "id": "C0017355", "name": "class i mhc", "pos": [ 253, 264 ] }
linkage of genetic markers from human chromosome 2q with susceptibility to leprosy or tuberculosis would support both the existence of a susceptibility gene in humans and the contention that this susceptibility gene is a homologue of the mouse bcg locus .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 160, 166 ] }
{ "id": "C0919453", "name": "susceptibility gene", "pos": [ 196, 215 ] }
the responses of hypaque-ficoll purified porcine ( p-pmn ) and human neutrophils ( h-pmn ) to stimulation with n-formyl-methionyl-leucyl-phenylalanine ( fmlp ) , c5a , phorbol myristate acetate ( pma ) , and calcium ionophore a23187 ( a23187 ) were compared by flow cytometrically measured changes in the cells ' forward ( fwd-sc ) ( a measure of shape/volume change ) and right angle ( 90 degrees-sc ) light scatter ( a measure of secretion ) , and in the distribution of the membrane potential sensitive fluorescent probe di-o-c ( 3 ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 63, 68 ] }
{ "id": "C3538902", "name": "di", "pos": [ 524, 526 ] }
neurodegenerative disease induced by the wild mouse ecotropic retrovirus is markedly accelerated by long terminal repeat and gag-pol sequences from nondefective friend murine leukemia virus .
organism_has_gene
{ "id": "C0035366", "name": "retrovirus", "pos": [ 62, 72 ] }
{ "id": "C0017360", "name": "pol", "pos": [ 129, 132 ] }
the association of human and bovine beta 2-microglobulins with detergent solubilized hla-a , b antigens was analyzed by a direct binding assay using radiolabeled beta 2-microglobulin and an immunoadsorbent containing a monoclonal antibody to the hla-a , b heavy chains .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 19, 24 ] }
{ "id": "C1420192", "name": "heavy chains", "pos": [ 256, 268 ] }
we examined some of the variables that were possible sources of the wide variability among and within human subjects in their steady-state visual evoked potentials ( vep ) in response to continuously counter-phased visual stimuli ( vertical bars ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 102, 107 ] }
{ "id": "C1707156", "name": "bars", "pos": [ 241, 245 ] }
the human analogue of v-sis can be distinguished from its rodent counterparts by southern blotting analysis of ecori-restricted dnas .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C0037207", "name": "v-sis", "pos": [ 22, 27 ] }
to obtain structural information on this molecule that can be used for comparison with known immunoglobulins and other surface molecules , we isolated the t cell-derived molecule by affinity chromatography using an antiserum raised against the monomeric fab fragment of a human waldenstrom macroglobulin and subjected the isolated molecule to either proteolysis using enzymes or cleavage with cyanogen bromide , followed by isolation of polypeptides which bore the fab- or heavy chain variable region ( vh ) -related antigenic markers .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 272, 277 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 473, 484 ] }
the results indicated that human dna contains several copies of the c-ras family and that c-ha-ras1 ( with intervening sequences ) was more highly conserved evolutionarily than was c-ha-ras2 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 27, 32 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 186, 189 ] }
a sequence of 14 nucleotides in one human diversity ( d ) minigene ( d2 ) is identical with a sequence in complementarity-determining region 2 ( cdr2 ) of one human gene for the variable region of the heavy chain of immunoglobulin .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 36, 41 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 201, 212 ] }
another human oncogene , c-sis , is located on chromosome 22 and was recently reported to be transferred to chromosome 9q+ in one cml patient .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 14, 22 ] }
another human oncogene , c-sis , is located on chromosome 22 and was recently reported to be transferred to chromosome 9q+ in one cml patient .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 8, 13 ] }
{ "id": "C0751995", "name": "c-sis", "pos": [ 25, 30 ] }
dominant transforming genes that were transferred to mouse nih3t3 cells by cellular dnas prepared from a chemically transformed human cell line ( mnng-hos ) , a human teratocarcinoma cell line ( pa1 ) , and a human pancreatic carcinoma cell line ( a1165 ) were characterized ( a ) analyzing the repetitive human dna sequences that were associated with the transforming gene and ( b ) determining their relationship to the oncogenes of the harvey ( rash ) and kirsten ( rask ) sarcoma viruses and to the human neuroblastoma transforming gene ( rasn ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 128, 133 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 422, 431 ] }
the human homologs of the raf ( mil ) oncogene are located on human chromosomes 3 and 4 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 38, 46 ] }
two human genes that are homologous to both the murine transforming gene ( oncogene ) v-raf and the chicken transforming gene v-mil have been mapped by means of human-rodent somatic cell hybrids to human chromosomes previously devoid of known oncogenes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 4, 9 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 243, 252 ] }
the nucleotide sequences of the six regions within the normal human cellular locus ( c-sis ) that correspond to the entire transforming region of the simian sarcoma virus ( ssv ) genome ( v-sis ) were determined .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 62, 67 ] }
{ "id": "C0751995", "name": "c-sis", "pos": [ 85, 90 ] }
the nucleotide sequences of the six regions within the normal human cellular locus ( c-sis ) that correspond to the entire transforming region of the simian sarcoma virus ( ssv ) genome ( v-sis ) were determined .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 62, 67 ] }
{ "id": "C0037207", "name": "v-sis", "pos": [ 188, 193 ] }
in contrast to other human tumors in which the c-myc gene and its transcript are greatly amplified , careful analysis of t ( 8 ; 14 ) burkitt cell lines indicates that the c-myc transcript is marginally , and in some cases not at all , increased by comparison to control lymphoblastoid cell lines .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 21, 26 ] }
{ "id": "C0086661", "name": "c-myc", "pos": [ 172, 177 ] }
they reacted specifically with multiple human monoclonal and polyclonal igm-rf , independent of any particular light or heavy chain amino acid sequence .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 40, 45 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 120, 131 ] }
in particular , the majority of murine plasmacytomas and human burkitt 's lymphomas contain a characteristic translocation which results in the juxtaposition of a cellular oncogene , c-myc , with the immunoglobulin heavy-chain gene locus , and this rearranged c-myc directs the synthesis of qualitatively and quantitatively abnormal transcripts which may have an aetiological role in the development of the transformed state in lymphoid malignancies .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 57, 62 ] }
{ "id": "C0086661", "name": "c-myc", "pos": [ 260, 265 ] }
based on the conservation of these human sequences in mouse fibroblast transformants , we conclude that the transforming ability of the oncogene activated in these tumors resides within a 43- to 46-kilobase-pair dna region .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 35, 40 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 136, 144 ] }
such a mechanism is in direct contrast to that of putative human oncogenes which are thought to induce tumorigenesis following activation or alteration .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 59, 64 ] }
{ "id": "C0029016", "name": "oncogenes", "pos": [ 65, 74 ] }
nucleotide sequences of the human alpha 1 and two allelic alpha 2 immunoglobulin heavy chain constant region genes are presented .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 28, 33 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 81, 92 ] }
the following procedures were associated with high rates of mouse embryo development and human pregnancies following ivf-et : adequate gassing and equilibration of the medium , double rinsing of pipettes and catheters used to handle embryos , use of a hepes-buffered medium for manipulating embryos in the absence of an atmosphere containing 5 % co2 , control of excessive temperature in the vicinity of the embryos , and et using medium containing 50 % patient 's serum .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 89, 94 ] }
{ "id": "C1419864", "name": "ivf", "pos": [ 117, 120 ] }
we tested the effectiveness of dihydroxyanthracenedione ( dhad ) on cell growth of two human pancreatic carcinoma cell lines mia paca-2 and panc-1 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 87, 92 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 125, 128 ] }
substantial amounts of human heavy chain accumulated in the transformed cells , and hla antigen was present at the cell surface .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 23, 28 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 29, 40 ] }
using human-mouse somatic cell hybrids containing different parts of chromosome 6 and a dna probe of the oncogene ( v-myb ) of avian myeloblastosis virus ( amv ) , we regionally mapped by southern blot techniques the human cellular myb ( c-myb ) protooncogene to 6q21 -- -- qter .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 6, 11 ] }
{ "id": "C0029016", "name": "oncogene", "pos": [ 251, 259 ] }
using human-mouse somatic cell hybrids containing different parts of chromosome 6 and a dna probe of the oncogene ( v-myb ) of avian myeloblastosis virus ( amv ) , we regionally mapped by southern blot techniques the human cellular myb ( c-myb ) protooncogene to 6q21 -- -- qter .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 6, 11 ] }
{ "id": "C0026900", "name": "v-myb", "pos": [ 116, 121 ] }
a human h4 histone gene was isolated and the nucleotide sequences of the mrna coding as well as the 5 ' and 3 ' flanking regions were determined .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 2, 7 ] }
{ "id": "C1425774", "name": "h4", "pos": [ 8, 10 ] }
similar blot hybridizations with a probe specific for the human n-ras oncogene detected a 9-kilobase ecori fragment in all cases .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 58, 63 ] }
{ "id": "C0027260", "name": "n-ras oncogene", "pos": [ 64, 78 ] }
two lines of evidence suggest that these genes may play a role in the development of human malignancy : ( 1 ) dna from a variety of human tumors transforms nih 3t3 mouse fibroblasts and the transforming genes from a number of carcinomas , sarcomas , and hematological malignancies have been identified as members of a family of genes , the ras family , closely related to the oncogenic genes of the harvey and kirsten murine sarcoma viruses ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 85, 90 ] }
{ "id": "C0029016", "name": "transforming genes", "pos": [ 190, 208 ] }
two lines of evidence suggest that these genes may play a role in the development of human malignancy : ( 1 ) dna from a variety of human tumors transforms nih 3t3 mouse fibroblasts and the transforming genes from a number of carcinomas , sarcomas , and hematological malignancies have been identified as members of a family of genes , the ras family , closely related to the oncogenic genes of the harvey and kirsten murine sarcoma viruses ;
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 85, 90 ] }
{ "id": "C0034678", "name": "ras", "pos": [ 340, 343 ] }
nag vibrios isolated from humans were sensitive to phages tepv in 45 % of cases and nag vibrios isolated from the environment , only in 29.9 % of cases .
organism_has_gene
{ "id": "C0086418", "name": "humans", "pos": [ 26, 32 ] }
{ "id": "C1709111", "name": "nag", "pos": [ 84, 87 ] }
a relation between this translocation and the location of the human oncogene c-sis on the chromosome 22 should be considered .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 62, 67 ] }
{ "id": "C0037207", "name": "oncogene", "pos": [ 68, 76 ] }
northern blot analysis has now shown that in the mcp6 hybrid , human class i genes , i.e. , hla-a , b , c , continued to be transcribed , and cellular levels of the transcripts were similar to , or only slightly lower than , levels in hybrids with a non-ec phenotype .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 63, 68 ] }
{ "id": "C0017355", "name": "class i genes", "pos": [ 69, 82 ] }
in the normal human ovary , surface epithelial cells , mature granulosa cells and lutein cells reacted with anti-hla-a , b , c ( hla ) and beta2-microglobulin antibodies but not with anti-ia ( ia-like , hla-dr ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 14, 19 ] }
{ "id": "C1417683", "name": "beta2", "pos": [ 139, 144 ] }
activated toxin preparations catalyzed adp-ribosylation of a protein ( mr = 40,000 ) present in cell membrane preparations obtained from human red blood cells and platelets , rat adipocytes , and cyc- s49 cells which are deficient in the adenylate cyclase regulatory component which is the substrate for cholera toxin .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 137, 142 ] }
{ "id": "C1426584", "name": "cyc", "pos": [ 248, 251 ] }
although the ch1 domains of the five classes of human heavy chains have the expected degree of homology ( approximately 30 % ) , the homology of the c delta 1 domains of the human and mouse chains is less than that exhibited by the ch1 domains of other pairs of human and mouse heavy chains .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 48, 53 ] }
{ "id": "C1420192", "name": "heavy", "pos": [ 278, 283 ] }
antigens associated with the epstein-barr virus ( ebv ) replicative cycle were found in the nucleus and cytoplasm of human placental , vero , bsc-1 , and owl monkey kidney cells transfected with ebv dna prepared from several different strains of virus .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 117, 122 ] }
{ "id": "C1420091", "name": "bsc", "pos": [ 142, 145 ] }
the influence of the following affinity labeling reagents on the binding of tritiated estradiol-17 beta ( e ) by human and calf uterine cytosols was studied : 11 beta-chloromethylestradiol ( org4333 ) , 2-azidoestradiol ( 2a-e ) , 4-azidoestradiol ( 4a-e ) , 3-azidohexestrol ( 3a-h ) , estradiol-17 beta 17-bromoacetate ( e-17brac ) , 6- [ o-carbo- ( 2'-chloroethoxy ) methyl ] oximinoestradiol ( 6-cmoetc1 ) , 17- [ o-carbo- ( 2'-chloroethoxy ) methyl ] oximinoestrone ( 17-cmoetcl ) , 2-di ( 2'-hydroxy-3'-chloropropyl ) aminoestradiol ( e-mustard ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 113, 118 ] }
{ "id": "C3538902", "name": "di", "pos": [ 534, 536 ] }
the amount of cross-hybridization between the human and rabbit alpha-globin mrna and the two alpha-globin cdnas was comparable to the cross-hybridization between the two beta-globin mrnas and the two beta-globin cdnas , indicating a similar degree of evolutionary divergence in the nucleotide sequences of the two globin genes .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 46, 51 ] }
{ "id": "C3825351", "name": "globin genes", "pos": [ 314, 326 ] }
however , treatment of sensitized cells with htla + c ' had little effects on the release of mif and lt .
organism_has_gene
{ "id": "C0086418", "name": "htla", "pos": [ 45, 49 ] }
{ "id": "C3538735", "name": "mif", "pos": [ 93, 96 ] }
purified human and rat plasma rbp have a single binding site for one molecule of retinol , alpha mobility on disc gel electrophoresis , and a molecular weight of approximately 20,000 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 9, 14 ] }
{ "id": "C1419320", "name": "plasma rbp", "pos": [ 23, 33 ] }
multiple common properties of human beta2-microglobulin and the common portion fragment derived from hl-a antigen molecules .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 30, 35 ] }
{ "id": "C1417683", "name": "beta2", "pos": [ 36, 41 ] }
evolutionary relationship between carboxyterminal region of a human alpha chain and other immunoglobulin heavy chain constant regions .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 62, 67 ] }
{ "id": "C1420192", "name": "heavy chain", "pos": [ 105, 116 ] }
although human toxicity from exposure to the plasticizer di ( 2-ethylhexyl ) phthalate ( dehp ) is unknown , reports of animal toxicity from dehp have stimulated extensive toxicological studies .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 9, 14 ] }
{ "id": "C3538902", "name": "di", "pos": [ 189, 191 ] }
human ia molecules carrying dc1 or br4x7 determinants are not homologous to murine i-e molecules .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 0, 5 ] }
{ "id": "C1415570", "name": "dc1", "pos": [ 28, 31 ] }
the extracellular matrix , prepared by extraction of confluent cultures of human lung wi-38 fibroblasts with a dipolar tonic detergent , contains four major glycoproteins : fibronectin , gp250 , gp170 , and gp140 .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 75, 80 ] }
{ "id": "C0376622", "name": "gp170", "pos": [ 195, 200 ] }
a striking similarity between the cytokeratins of human basal-cell epitheliomas and those of the pilosebaceous tract has been found : all three major cytokeratins ( mol wts 58,000 ; 50,000 ; 46,000 ) of the tumor cells are also expressed in hair-follicle epithelium .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 50, 55 ] }
{ "id": "C1705714", "name": "wts", "pos": [ 169, 172 ] }
because a direct relationship exists between the ed50 values obtained from these discrimination studies and human hallucinogenic potencies , the discriminative stimulus paradigm , with dom as a training drug , appears to be a useful tool for comparing the quantitative and qualitative ( dom-like ) effects produced by certain hallucinogenic agents .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 108, 113 ] }
{ "id": "C3539821", "name": "dom", "pos": [ 287, 290 ] }
we investigated the presumed anion-binding domain of the anion-transport protein from human erythrocyte membranes , using 2,6-di-iodo-4-sulphophenyl isothiocyanate , an inhibitor of anion transport .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 86, 91 ] }
{ "id": "C3538902", "name": "di", "pos": [ 126, 128 ] }
cultured human pancreatic carcinoma cells ( mia paca-2 ) have been shown previously to be very sensitive to e. coli l-asparaginase ( ec ii ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 9, 14 ] }
{ "id": "C1417154", "name": "mia", "pos": [ 44, 47 ] }
by thin-layer chromatographic behaviour and gas-liquid chromatographic determinations the main gangliosides in human kidney are n-acetylneuraminyllactosylceramide ( 74 % of total ) and di-n-acetylneuraminyllactosylceramide ( 19 % of total ) .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 111, 116 ] }
{ "id": "C3538902", "name": "di", "pos": [ 185, 187 ] }
polyglutamate derivatives of labelled methotrexate were formed by human lymphocytes with mean amounts of 4-5 % of di- , 1-5 % of tri- , 1-0 % of tetra- and 0-7 % of penta-glutamate derivatives being formed over the period 48-72h of culture .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 66, 71 ] }
{ "id": "C3538902", "name": "di", "pos": [ 114, 116 ] }
while they have no effect on the transport of na in human and cat red cells , in dog red cells adenosine and its mono- , di- and triphosphorylated forms were found to increase na-influx .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 52, 57 ] }
{ "id": "C3538902", "name": "di", "pos": [ 121, 123 ] }
incubation of human monocytes with alpha-l-fucosidase , a glycosidase which cleaves terminal l-fucose moieties , reversibly prevented their response to mif .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 14, 19 ] }
{ "id": "C3538735", "name": "mif", "pos": [ 152, 155 ] }
preliminary analysis of the 5'-termini of the 18-s rna from human , rat , xenopus and sea urchin cells revealed the same pup ( np ) gp -- - type 5'-terminal structure , supporting the above hypothesis .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 60, 65 ] }
{ "id": "C1706141", "name": "np", "pos": [ 127, 129 ] }
the guinea pig protein closely resembles the human and rabbit beta2-microglobulins in amino acid composition , charge , molecular size , and also in the presence of an apparently analogous disulfide loop .
organism_has_gene
{ "id": "C0086418", "name": "human", "pos": [ 45, 50 ] }
{ "id": "C1417683", "name": "beta2", "pos": [ 62, 67 ] }