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because spantide attenuated the effect of capsaicin , increased insufflation pressure and specific airway resistance due to capsaicin challenge in both unanesthetized and anesthetized guinea pigs may be attributed , at least in part , to capsaicin 's induction of substance p release or the release of other tachykinins .
NA
{ "id": "C0038585", "name": "substance p", "pos": [ 264, 275 ] }
{ "id": "C0039241", "name": "tachykinins", "pos": [ 308, 319 ] }
lymphoepithelioid cell lymphoma , defined as a lymphoma of cd4+ t lymphocytes , marks the border between hodgkin 's disease and the non-hodgkin 's lymphomas .
NA
{ "id": "C0024299", "name": "lymphoma", "pos": [ 23, 31 ] }
{ "id": "C0024305", "name": "non-hodgkin 's lymphomas", "pos": [ 132, 156 ] }
the antigens used were two toxoids , tetanus and diphtheria , three bacterial antigens , streptococcus , tuberculin and proteus and two fungal antigens , candida and trichophyton .
NA
{ "id": "C0003320", "name": "antigens", "pos": [ 4, 12 ] }
{ "id": "C0003321", "name": "bacterial antigens", "pos": [ 68, 86 ] }
the antigens used were two toxoids , tetanus and diphtheria , three bacterial antigens , streptococcus , tuberculin and proteus and two fungal antigens , candida and trichophyton .
NA
{ "id": "C0003320", "name": "antigens", "pos": [ 4, 12 ] }
{ "id": "C0003330", "name": "fungal antigens", "pos": [ 136, 151 ] }
all patients underwent skin testing with house dust , dermatophagoides pternyssinus ( dpt ) , cat and dog allergens , grass , tree and weed pollens , and moulds .
NA
{ "id": "C0524581", "name": "house dust", "pos": [ 41, 51 ] }
{ "id": "C0002092", "name": "allergens", "pos": [ 106, 115 ] }
for the purposes of the study , supplementary agents were restricted to fentanyl , alfentanil or nitrous oxide ;
NA
{ "id": "C0015846", "name": "fentanyl", "pos": [ 72, 80 ] }
{ "id": "C0002026", "name": "alfentanil", "pos": [ 83, 93 ] }
blood samples were taken for analysis of epinephrine , norepinephrine , serotonin , and arterial and mixed-venous blood gases .
NA
{ "id": "C0014563", "name": "epinephrine", "pos": [ 41, 52 ] }
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 55, 69 ] }
purification of the sample with alumina adsorption alone , a method usually used for purification of catecholamines and dopa , was ineffective : epinephrine and norepinephrine , which are present in high concentrations , interfered with an accurate determination of dopa , and dopamine , which is retained strongly on the reverse-phase column , interfered with a rapid analysis .
NA
{ "id": "C0007412", "name": "catecholamines", "pos": [ 101, 115 ] }
{ "id": "C0013023", "name": "dopa", "pos": [ 277, 281 ] }
purification of the sample with alumina adsorption alone , a method usually used for purification of catecholamines and dopa , was ineffective : epinephrine and norepinephrine , which are present in high concentrations , interfered with an accurate determination of dopa , and dopamine , which is retained strongly on the reverse-phase column , interfered with a rapid analysis .
NA
{ "id": "C0014563", "name": "epinephrine", "pos": [ 145, 156 ] }
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 161, 175 ] }
after purification with columns of weak cation-exchange resins such as bio-rex 70 or amberlite cg-50 followed by alumina adsorption , most of the epinephrine and norepinephrine was removed and dopamine was eliminated .
NA
{ "id": "C0014563", "name": "epinephrine", "pos": [ 146, 157 ] }
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 162, 176 ] }
conditions for the preparative elution of proteins from immobilon membranes after transfer of proteins to this matrix from sodium dodecyl sulfate-polyacrylamide gels have been established .
NA
{ "id": "C0033684", "name": "proteins", "pos": [ 42, 50 ] }
{ "id": "C0025252", "name": "membranes", "pos": [ 66, 75 ] }
oligosaccharides were eluted from a hi-chrom 5s ods ( c18 ) column using mixtures of acetonitrile and buffers containing 40 mm ammonium phosphate and 1 mm tetrabutylammonium phosphate .
NA
{ "id": "C0051724", "name": "ammonium phosphate", "pos": [ 127, 145 ] }
{ "id": "C0031603", "name": "phosphate", "pos": [ 174, 183 ] }
a comparison of the elution profiles obtained in the ion-pairing chromatography procedure with profiles obtained by anion-exchange high-pressure liquid chromatography profiles showed markedly better overall resolution by the ion-pairing procedure .
NA
{ "id": "C0003075", "name": "anion", "pos": [ 116, 121 ] }
{ "id": "C0022023", "name": "ion", "pos": [ 225, 228 ] }
the method employs a citrate buffer gradient to resolve several inositol phosphates including inositol 1-phosphate , inositol 1,4-bisphosphate ( ip2 ) , and inositol 1,4,5-trisphosphate ( ip3 ) , as well as some of the isomers of these compounds .
NA
{ "id": "C0021554", "name": "inositol phosphates", "pos": [ 64, 83 ] }
{ "id": "C0021547", "name": "inositol", "pos": [ 157, 165 ] }
the method employs a citrate buffer gradient to resolve several inositol phosphates including inositol 1-phosphate , inositol 1,4-bisphosphate ( ip2 ) , and inositol 1,4,5-trisphosphate ( ip3 ) , as well as some of the isomers of these compounds .
NA
{ "id": "C0021554", "name": "inositol phosphates", "pos": [ 64, 83 ] }
{ "id": "C0021549", "name": "ip3", "pos": [ 188, 191 ] }
a method for dna fragmentation by h2o2 in the dna alkaline elution procedure is described .
NA
{ "id": "C0000702", "name": "a", "pos": [ 0, 1 ] }
{ "id": "C0012854", "name": "dna", "pos": [ 46, 49 ] }
a single-step enzyme assay using [ 14c ] palmitic acid and bacterial acyl-coenzyme a synthetase ( ec 6.2.1.3 ) is described for the determination of reduced coenzyme a ( coash ) levels in liver samples .
NA
{ "id": "C0001374", "name": "acyl-coenzyme", "pos": [ 69, 82 ] }
{ "id": "C0009226", "name": "coenzyme", "pos": [ 157, 165 ] }
immunological detection of b-dna to z-dna transition of polynucleotides by immobilization of the dna conformation on a solid support .
NA
{ "id": "C0012854", "name": "dna", "pos": [ 29, 32 ] }
{ "id": "C0043442", "name": "z-dna", "pos": [ 36, 41 ] }
from the transition curves , we determined the concentration of the counterions at the midpoint of b-dna to z-dna transition .
NA
{ "id": "C0012854", "name": "dna", "pos": [ 101, 104 ] }
{ "id": "C0043442", "name": "z-dna", "pos": [ 108, 113 ] }
and a decreased ratio of very low density lipoproteins to total lipoproteins , suggesting that changes in types of hyperlipoproteinemia may be associated with the onset of myocardial infarction .
NA
{ "id": "C0023825", "name": "very low", "pos": [ 25, 33 ] }
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 64, 76 ] }
two major classes of drugs are considered , those that stimulate cell membrane adenylcyclase , i.e . beta-agonists ( dopamine , dobutamine and dopexamine ) and alpha-agonists ( glucagon , forskolin , calcium agonists ) and those that inhibit the cellular phosphodiesterases , i.e . bipyridine derivatives ( amrinone and milrinone ) and imidazolone derivatives ( fenoximone and piroximone ) .
NA
{ "id": "C0002697", "name": "amrinone", "pos": [ 307, 315 ] }
{ "id": "C0128513", "name": "milrinone", "pos": [ 320, 329 ] }
dobutamine exerts a potent positive inotropic action , with little effect on vascular tone and less tachycardia than with other catecholamines , resulting in only a slight increase in myocardial oxygen consumption .
NA
{ "id": "C0012963", "name": "dobutamine", "pos": [ 0, 10 ] }
{ "id": "C0007412", "name": "catecholamines", "pos": [ 128, 142 ] }
the effect of nalbuphine on the common bile duct pressure of ten women who had cholecystectomy and common duct exploration for cholelithiasis and choledocholithiasis was studied .
NA
{ "id": "C0008350", "name": "cholelithiasis", "pos": [ 127, 141 ] }
{ "id": "C0701818", "name": "choledocholithiasis", "pos": [ 146, 165 ] }
patients with cholelithiasis had a significantly greater weight loss ( 130 +/- 61.0 lbs ) than those without stones ( 109 +/- 59.9 lbs ) p = 0.04 .
NA
{ "id": "C0008350", "name": "cholelithiasis", "pos": [ 14, 28 ] }
{ "id": "C0006736", "name": "stones", "pos": [ 109, 115 ] }
the two monoclonal antibodies hrs-1 and hrs-2 , which were obtained after immunization with the hodgkin 's derived cell line l428 , detect different epitopes on a 120 kd antigen .
NA
{ "id": "C0003316", "name": "epitopes", "pos": [ 149, 157 ] }
{ "id": "C0003320", "name": "antigen", "pos": [ 170, 177 ] }
a series of poly ( adp-ribose ) synthesis inhibitors was evaluated for their ability to potentiate the cytotoxicity of bleomycin compared with their inhibition of poly ( adp-ribose ) synthesis in l1210 cultured cells .
NA
{ "id": "C0001461", "name": "adp-ribose", "pos": [ 19, 29 ] }
{ "id": "C0032403", "name": "poly ( adp-ribose", "pos": [ 12, 29 ] }
one of the strains tested , however , a clinical isolate of t. pallidum designated street strain 14 , was found to exhibit high-level resistance to erythromycin and a closely related macrolide , roxithromycin ( ru 965 ) .
NA
{ "id": "C0014806", "name": "erythromycin", "pos": [ 148, 160 ] }
{ "id": "C0035891", "name": "roxithromycin", "pos": [ 195, 208 ] }
percentages of study isolates susceptible to cefamandole , cefaclor , cephalothin , and cephalexin were 98.7 , 94.5 , 87.3 , and 43.3 % , respectively .
NA
{ "id": "C0007537", "name": "cefaclor", "pos": [ 59, 67 ] }
{ "id": "C0007716", "name": "cephalexin", "pos": [ 88, 98 ] }
the in vitro susceptibility of human isolates of pasteurella multocida to oral antimicrobial agents from our current study and from a review of the literature suggests that dicloxacillin ( oxacillin ) , erythromycin , clindamycin , cephalexin , cefaclor , and cefadroxil should not be used for empiric therapy of animal bite wounds .
NA
{ "id": "C0007716", "name": "cephalexin", "pos": [ 232, 242 ] }
{ "id": "C0007537", "name": "cefaclor", "pos": [ 245, 253 ] }
the in vitro susceptibility of human isolates of pasteurella multocida to oral antimicrobial agents from our current study and from a review of the literature suggests that dicloxacillin ( oxacillin ) , erythromycin , clindamycin , cephalexin , cefaclor , and cefadroxil should not be used for empiric therapy of animal bite wounds .
NA
{ "id": "C0007716", "name": "cephalexin", "pos": [ 232, 242 ] }
{ "id": "C0007538", "name": "cefadroxil", "pos": [ 260, 270 ] }
all the isolates were resistant to ampicillin , cefazolin , cefuroxime , cefamandole , cefotetan , ceftriaxone , cefotaxime , aztreonam , amdinocillin , and temocillin .
NA
{ "id": "C0007562", "name": "cefuroxime", "pos": [ 60, 70 ] }
{ "id": "C0007554", "name": "cefotaxime", "pos": [ 113, 123 ] }
all the isolates were resistant to ampicillin , cefazolin , cefuroxime , cefamandole , cefotetan , ceftriaxone , cefotaxime , aztreonam , amdinocillin , and temocillin .
NA
{ "id": "C0007561", "name": "ceftriaxone", "pos": [ 99, 110 ] }
{ "id": "C0007554", "name": "cefotaxime", "pos": [ 113, 123 ] }
with the exception of cephalothin , which was markedly toxic to kidney cells in vitro , a good correlation between in vitro toxicity and in vivo nephrotoxicity was obtained , yielding the following toxicity rank order : ceftazidime less than cefazolin approximately cefoperazone less than cephaloglycin approximately cephaloridine .
NA
{ "id": "C0007559", "name": "ceftazidime", "pos": [ 220, 231 ] }
{ "id": "C0007727", "name": "cephaloridine", "pos": [ 317, 330 ] }
the 26k , 27.5k , and 28.5k antigens present in the om of cells grown in vitro ( but not in vivo ) were recognized by antibodies raised against bacteria cultivated in vitro under conditions of iron depletion , but were not recognized by antisera raised against bacteria harvested directly from infections .
NA
{ "id": "C0003241", "name": "antibodies", "pos": [ 118, 128 ] }
{ "id": "C0020960", "name": "antisera", "pos": [ 237, 245 ] }
clavulanic acid combined with amoxicillin and ticarcillin and sulbactam combined with ampicillin resulted in synergistic activity against all beta-lactamase-positive organisms .
NA
{ "id": "C0002645", "name": "amoxicillin", "pos": [ 30, 41 ] }
{ "id": "C0002680", "name": "ampicillin", "pos": [ 86, 96 ] }
the rank order for stabilizing the low affinity state was gpp ( nh ) p greater than gtp greater than gdp much greater than gmp = no nucleotides .
NA
{ "id": "C0018366", "name": "gpp ( nh ) p", "pos": [ 58, 70 ] }
{ "id": "C0018353", "name": "gtp", "pos": [ 84, 87 ] }
the gamma-crystallin fraction of the soluble lens proteins was separated into five distinct polypeptides and characterized by isoelectric focusing , amino acid composition , and n-terminal sequence analysis to 25 residues .
NA
{ "id": "C0017030", "name": "gamma-crystallin", "pos": [ 4, 20 ] }
{ "id": "C0010422", "name": "lens proteins", "pos": [ 45, 58 ] }
a panel of monoclonal antibodies was raised against a novel glycosphingolipid that contains a terminal 3-fl structure ( structure 1 ) : ( formula : see text ) surprisingly , most of these antibodies reacted with the internal galactosylgloboside structure rather than with the terminal 3-fl epitope .
NA
{ "id": "C0003250", "name": "monoclonal", "pos": [ 11, 21 ] }
{ "id": "C0003241", "name": "antibodies", "pos": [ 188, 198 ] }
the functional affinity of igm rheumatoid factors ( rf ) was measured in 31 patients with rheumatoid arthritis ( ra ) , 24 with systemic lupus erythematosus ( sle ) , 13 with sjogren 's syndrome ( ss ) , and in 13 seropositive healthy individuals .
NA
{ "id": "C0003873", "name": "rheumatoid arthritis", "pos": [ 90, 110 ] }
{ "id": "C1527336", "name": "sjogren 's syndrome", "pos": [ 175, 194 ] }
the second line activity of sulphamethoxazole , in the form of cotrimoxazole ( ctz ) , has been investigated by treatment of 13 patients with ra for 24 weeks with ctz ( 480 mg three times a day ) .
NA
{ "id": "C0038689", "name": "sulphamethoxazole", "pos": [ 28, 45 ] }
{ "id": "C0041044", "name": "ctz", "pos": [ 163, 166 ] }
murine skin fibroblasts have been studied in our laboratory , using different systems : ( 1 ) primary cultures isolated from peeled skins of mouse embryos , ( 2 ) mouse derms analysed in the animals , ( 3 ) cultivated explants of skins , ( 4 ) serial sub-cultures of fibroblasts isolated from these explants , ( 5 ) cells cultivated comparably on plane substrates ( glass , plastic , collagen films ) and on three-dimensional matrices ( collagen fibres ) .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 12, 23 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 316, 321 ] }
ten patients aged from 60 to 73 years presenting with horton 's disease or polymyalgia rheumatica had arteritis of the upper limbs .
NA
{ "id": "C0039483", "name": "horton 's disease", "pos": [ 54, 71 ] }
{ "id": "C0003860", "name": "arteritis", "pos": [ 102, 111 ] }
lipids , lipoproteins and hepatic 3-hydroxy-3-methylglutaryl coa reductase activities of rabbits during adaptation to atherogenic semi-purified diets .
NA
{ "id": "C0023779", "name": "lipids", "pos": [ 0, 6 ] }
{ "id": "C0023820", "name": "lipoproteins", "pos": [ 9, 21 ] }
electron-microscopic examination revealed myoid-type cells with clusters of myofilaments and z-type material , cells resembling fibroblasts , osteoclasts and lipocytes .
NA
{ "id": "C0007634", "name": "cells", "pos": [ 53, 58 ] }
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 128, 139 ] }
pharmacological analysis of these enkephalin actions on conditioning suggests that the [ leu ] enkephalin acts through a delta opioid receptor which is located outside the blood-brain barrier .
NA
{ "id": "C0014298", "name": "enkephalin", "pos": [ 34, 44 ] }
{ "id": "C0014294", "name": "leu ] enkephalin", "pos": [ 89, 105 ] }
we conclude that an important proportion of human kininogens contain hydroxyproline instead of proline in position three of the bradykinin moiety .
NA
{ "id": "C0020388", "name": "hydroxyproline", "pos": [ 69, 83 ] }
{ "id": "C0033382", "name": "proline", "pos": [ 95, 102 ] }
the effects of various protein kinase c ( pkc ) inhibitors on nadph oxidase ( no ) activation by the phorbol ester pma and by the chemotactic peptide fmlp were studied .
NA
{ "id": "C0031586", "name": "phorbol ester", "pos": [ 101, 114 ] }
{ "id": "C0039654", "name": "pma", "pos": [ 115, 118 ] }
active human thyroid-stimulating hormone ( htsh ) was produced by xenopus laevis oocytes following injection of an mrna mixture of htsh beta and alpha subunits synthesized by t3 rna polymerase .
NA
{ "id": "C0040160", "name": "thyroid-stimulating hormone", "pos": [ 13, 40 ] }
{ "id": "C0076641", "name": "htsh beta", "pos": [ 131, 140 ] }
albumin concentration in the blood , and its synthesis by the liver , decrease in the rat during the acute-phase response to inflammation .
NA
{ "id": "C0001349", "name": "acute-phase response", "pos": [ 101, 121 ] }
{ "id": "C0021368", "name": "inflammation", "pos": [ 125, 137 ] }
dna binding proteins operate in an intracellular environment of low chloride concentration , yet in vitro assays of the activities of these proteins are often performed in isotonic chloride-containing solutions .
NA
{ "id": "C0012940", "name": "dna binding proteins", "pos": [ 0, 20 ] }
{ "id": "C0033684", "name": "proteins", "pos": [ 140, 148 ] }
previously , the activity of bacterial dna-binding proteins was found to be enhanced in potassium-containing solutions in which the anion glutamate ( glu ) was substituted for chloride .
NA
{ "id": "C0003075", "name": "anion", "pos": [ 132, 137 ] }
{ "id": "C0008203", "name": "chloride", "pos": [ 176, 184 ] }
all these findings suggest that in mice , there is a male-specific renal 3-meo-aab activation enzyme ( s ) , a cytochrome p-450 isozyme ( s ) , which is different , at least in proportion and/or in nature , from hepatic cytochrome p-450 isozymes .
NA
{ "id": "C0014442", "name": "enzyme", "pos": [ 94, 100 ] }
{ "id": "C0022173", "name": "isozymes", "pos": [ 237, 245 ] }
in order to investigate the possibility that there may be two conformationally distinct dopamine d1 binding sites , the effect of lysine-modifying agents on striatal dopamine d1 receptors was investigated .
NA
{ "id": "C0034798", "name": "dopamine", "pos": [ 88, 96 ] }
{ "id": "C0058697", "name": "dopamine d1 receptors", "pos": [ 166, 187 ] }
the study consisted in a randomized comparative trial on the peri-/postoperative thrombotic prophylaxis with lmwh 2,500 u s.c. once daily and unfractionated heparin ( ufh ) 5,000 u s.c. twice daily .
NA
{ "id": "C0019139", "name": "lmwh", "pos": [ 109, 113 ] }
{ "id": "C0019134", "name": "unfractionated", "pos": [ 142, 156 ] }
under standard prophylaxis with ufh/dhe twice a day 14 patients suffered postoperative thromboses , while with lmwh/dhe once a day only 4 patients suffered thromboses ( p less than 0.025 ) .
NA
{ "id": "C0019134", "name": "ufh", "pos": [ 32, 35 ] }
{ "id": "C0019139", "name": "lmwh", "pos": [ 111, 115 ] }
a further characteristic distinguishing between the central and peripheral alpha-adrenoceptors regulating cardiovascular tone was a distinct inability of some derivatives active at peripheral cardiac presynaptic ( pithed rats ) and central ( young chicks ) alpha 2-adrenoceptors to reduce blood pressure in anaesthetized rats .
NA
{ "id": "C0034784", "name": "alpha-adrenoceptors", "pos": [ 75, 94 ] }
{ "id": "C0001639", "name": "alpha 2-adrenoceptors", "pos": [ 257, 278 ] }
in studies on structure-activity relationships among impromidine-like histamine h2-receptor agonists , the synthesis of impromidine analogous guanidines led to the corresponding intermediate cyanoguanidines .
NA
{ "id": "C0063440", "name": "impromidine", "pos": [ 53, 64 ] }
{ "id": "C0018320", "name": "guanidines", "pos": [ 196, 206 ] }
the third peak ( p3 ) eluted with 290 mm nacl represented 4.3 % of total hdl-c and 6.0 % of total hdl protein , and contained apo e ( 25 % of its protein ) .
NA
{ "id": "C0023814", "name": "3", "pos": [ 18, 19 ] }
{ "id": "C0023821", "name": "hdl", "pos": [ 98, 101 ] }
the rates of accumulation of apo a-i and albumin in the medium were 22 ng/mg cell protein per h and 1.2 micrograms/mg cell protein per h , respectively .
NA
{ "id": "C0001924", "name": "albumin", "pos": [ 41, 48 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 123, 130 ] }
the time courses for diet-induced changes in plasma lipids and the lipid ( cholesteryl ester/triglyceride ) transfer activity of lipoprotein-deficient plasma ( lta ) were determined in rabbits fed a variety of hyperlipidemic diets .
NA
{ "id": "C0023779", "name": "lipid", "pos": [ 52, 57 ] }
{ "id": "C0023820", "name": "lipoprotein", "pos": [ 129, 140 ] }
it was hypothesized that such pentobarbital blockage of morphine tolerance represents an instance of state-dependent learning : the barbiturate alters predrug cues so that the final , barbiturate-signaled morphine administration is presented in the context of different signals than those accompanying pretest administrations of the opiate .
NA
{ "id": "C0030883", "name": "pentobarbital", "pos": [ 30, 43 ] }
{ "id": "C0004745", "name": "barbiturate", "pos": [ 184, 195 ] }
results of the present experiments confirmed the finding that pentobarbital interferes with the expression of morphine tolerance in rats that had not previously received barbiturate-opiate pairings .
NA
{ "id": "C0030883", "name": "pentobarbital", "pos": [ 62, 75 ] }
{ "id": "C0004745", "name": "barbiturate", "pos": [ 170, 181 ] }
moreover , if pretest morphine administrations were signaled by pentobarbital , omission of the barbiturate cue on the test session attenuated tolerance .
NA
{ "id": "C0030883", "name": "pentobarbital", "pos": [ 64, 77 ] }
{ "id": "C0004745", "name": "barbiturate", "pos": [ 96, 107 ] }
red blood cells of adult western painted turtles ( chrysemys picta bellii ) contain two hemoglobin components : hba ( alpha a2 beta 2 ) and hbd ( alpha d2 beta 2 ) .
NA
{ "id": "C0019046", "name": "hemoglobin", "pos": [ 88, 98 ] }
{ "id": "C0019016", "name": "hba", "pos": [ 112, 115 ] }
in comparison to other vertebrate hemoglobins there is a surprising similarity of the sequences to those of bird hemoglobins . alpha a- as well as alpha d-chains show larger homologies to chains of the same type in different species than alpha a- and alpha d-chains to each other in the same species .
NA
{ "id": "C0019046", "name": "hemoglobins", "pos": [ 34, 45 ] }
{ "id": "C0019016", "name": "hemoglobins", "pos": [ 113, 124 ] }
second , isotype determines antibody functions , effector ( neutralization , antibody-dependent complement , or cell-mediated cytotoxicity ) as well as blocking functions .
NA
{ "id": "C0021017", "name": "isotype", "pos": [ 9, 16 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 77, 85 ] }
in this report , we show that 2',3'-dideoxynucleosides including 3'-azido-2',3'-dideoxythymidine ( azt ) can significantly inhibit the infectivity and/or cytopathic effect of these retroviruses in vitro .
NA
{ "id": "C0012136", "name": "dideoxynucleosides", "pos": [ 36, 54 ] }
{ "id": "C0043474", "name": "azt", "pos": [ 99, 102 ] }
the amino acid sequence coded by the cdna for hepsin shows a high degree of identity to pancreatic trypsin and other serine proteases present in plasma .
NA
{ "id": "C0041236", "name": "trypsin", "pos": [ 99, 106 ] }
{ "id": "C0036734", "name": "serine proteases", "pos": [ 117, 133 ] }
it also exhibits features characteristic of zymogens to serine proteases in that it contains a cleavage site for protease activation and the highly conserved regions surrounding the his , asp , and ser residues that participate in enzyme catalysis .
NA
{ "id": "C0036734", "name": "serine proteases", "pos": [ 56, 72 ] }
{ "id": "C0030946", "name": "protease", "pos": [ 113, 121 ] }
all effects of glutamate and glycine on [ 3h ] tcp binding kinetics were blocked by the competitive nmda receptor antagonist ap-5 [ d- ( - ) -2-amino-5-phosphovaleric acid ] .
NA
{ "id": "C0017890", "name": "glycine", "pos": [ 29, 36 ] }
{ "id": "C0002520", "name": "amino", "pos": [ 144, 149 ] }
the major membrane lipid species identified in s. cremoris were acidic phospholipids ( phosphatidylglycerol and cardiolipin ) , glycolipids , and glycerophosphoglycolipids .
NA
{ "id": "C0025249", "name": "membrane lipid", "pos": [ 10, 24 ] }
{ "id": "C0031676", "name": "phospholipids", "pos": [ 71, 84 ] }
we have developed a system for targeting foreign dna to hepatocytes in vitro using a soluble dna carrier that takes advantage of receptor-mediated endocytosis to achieve internalization .
NA
{ "id": "C0000702", "name": "a", "pos": [ 4, 5 ] }
{ "id": "C0012854", "name": "dna", "pos": [ 93, 96 ] }
we tested this complex using a model system consisting of human hepatoma cell line hep g2 [ asialoglycoprotein receptor ( + ) ] , hepatoma sk-hep 1 , imr-90 fibroblasts , and uterine smooth muscle [ receptor ( - ) ] cells .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 157, 168 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 216, 221 ] }
the recognition and repair of the helix-stabilizing and relatively nondistortive cc-1065- ( n3-adenine ) -dna adduct by uvrabc nuclease has been investigated both in vivo with phi x174 rfi dna by a transfection assay and in vitro by a site-directed adduct in a 117 base pair fragment from m13mp1 .
NA
{ "id": "C0242958", "name": "dna adduct", "pos": [ 106, 116 ] }
{ "id": "C0012854", "name": "dna", "pos": [ 189, 192 ] }
tissue plasminogen activator ( tpa ) was covalently linked by disulfide bonds to a monoclonal antibody specific for the amino terminus of the beta chain of fibrin ( antibody 59d8 ) .
NA
{ "id": "C0003250", "name": "monoclonal antibody", "pos": [ 83, 102 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 165, 173 ] }
conjugation of tpa or uk to antibody 59d8 produced a 3.2-4.5-fold enhancement in clot lysis in human plasma over that of the respective unconjugated plasminogen activator .
NA
{ "id": "C0032143", "name": "tpa", "pos": [ 15, 18 ] }
{ "id": "C0032144", "name": "plasminogen activator", "pos": [ 149, 170 ] }
we have shown previously that substitutions of glu-190 with either glutamine or aspartate resulted in a complete loss of sulfate activation and in decreased activities ;
NA
{ "id": "C0061472", "name": "glu", "pos": [ 47, 50 ] }
{ "id": "C0017797", "name": "glutamine", "pos": [ 67, 76 ] }
this suggests that both direct protein-lipid electrostatic interactions and the ionic double layer , which gives rise to a low local ph around anionic vesicles , contribute to the shift in the transition ph .
NA
{ "id": "C0022023", "name": "ionic", "pos": [ 80, 85 ] }
{ "id": "C0003075", "name": "anionic", "pos": [ 143, 150 ] }
dcmp can protect the enzyme against nem inactivation , but the dihydrofolate analogues methotrexate and aminopterin alone do not afford similar protection .
NA
{ "id": "C0025677", "name": "methotrexate", "pos": [ 87, 99 ] }
{ "id": "C0002583", "name": "aminopterin", "pos": [ 104, 115 ] }
compared to dcmp alone , dcmp plus either methotrexate or aminopterin greatly enhances protection against nem inactivation .
NA
{ "id": "C0025677", "name": "methotrexate", "pos": [ 42, 54 ] }
{ "id": "C0002583", "name": "aminopterin", "pos": [ 58, 69 ] }
the genes encoding anabaena and e. coli thioredoxin were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate hybrid genes , coding for two chimeric thioredoxins .
NA
{ "id": "C0033684", "name": "proteins", "pos": [ 154, 162 ] }
{ "id": "C0039938", "name": "thioredoxins", "pos": [ 214, 226 ] }
these proteins are designated anabaena-e. coli ( a-e ) thioredoxin for the construct with the anabaena sequence from the n-terminus to the middle of the active site and the e. coli sequence to the c-terminus , and e. coli-anabaena ( e-a ) for the opposite construct .
NA
{ "id": "C0033684", "name": "proteins", "pos": [ 6, 14 ] }
{ "id": "C0039938", "name": "thioredoxin", "pos": [ 55, 66 ] }
as was found in murine c5 , the corresponding thiol ester region of human c5 contained several conserved amino acids , but the critical cysteine and glutamine residues which give rise to the intramolecular thiol ester bond in c3 , c4 , and alpha 2-macroglobulin were absent in c5 , having been replaced by serine and alanine , respectively .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 105, 116 ] }
{ "id": "C0001898", "name": "alanine", "pos": [ 317, 324 ] }
differential absorption spectroscopy and chemical titration demonstrated that human apo a-iv contains six tyrosine residues , four of which are buried in the hydrophobic interior of the protein and two of which are exposed on the protein surface .
NA
{ "id": "C0033684", "name": "protein", "pos": [ 186, 193 ] }
{ "id": "C0025252", "name": "protein surface", "pos": [ 230, 245 ] }
multiple forms of the acute-phase serum protein saa were isolated from the lipoprotein fraction of plasma from a single individual .
NA
{ "id": "C0001347", "name": "acute-phase", "pos": [ 22, 33 ] }
{ "id": "C0002723", "name": "saa", "pos": [ 48, 51 ] }
misincorporation opposite template bu residues catalyzed by maloney murine leukemia virus dna polymerase and dna polymerase beta ( novikoff hepatoma ) also increased with ph , but for these two enzymes , there was no apparent dependence on sequence context .
NA
{ "id": "C0012892", "name": "dna polymerase", "pos": [ 90, 104 ] }
{ "id": "C0525039", "name": "dna polymerase beta", "pos": [ 109, 128 ] }
the surface density of the fluorescent probe n- ( lissamine rhodamine b sulfonyl ) dipalmitoylphosphatidylcholine is the same in the two lipid leaflets of phosphatidylcholine bilayers containing the probe .
NA
{ "id": "C0000039", "name": "dipalmitoylphosphatidylcholine", "pos": [ 83, 113 ] }
{ "id": "C1959616", "name": "phosphatidylcholine", "pos": [ 155, 174 ] }
the influence of the detergent/protein and lipid/protein ratios on the yield of membrane bound enzyme is described .
NA
{ "id": "C0033684", "name": "protein", "pos": [ 31, 38 ] }
{ "id": "C0025252", "name": "protein", "pos": [ 49, 56 ] }
the penultimate residue with dipeptidyl peptidase iv can be , beside proline and alanine , dehydroproline , hydroxyproline and pipecolic acid .
NA
{ "id": "C0033382", "name": "proline", "pos": [ 69, 76 ] }
{ "id": "C0020388", "name": "hydroxyproline", "pos": [ 108, 122 ] }
apob from chylomicrons analyzed within 5 h of blood collection showed a single band with mobility identical to that of apob ( apob-100 ) in low-density lipoproteins .
NA
{ "id": "C0103839", "name": "apob", "pos": [ 0, 4 ] }
{ "id": "C0003593", "name": "apob", "pos": [ 126, 130 ] }
apob from chylomicrons analyzed within 5 h of blood collection showed a single band with mobility identical to that of apob ( apob-100 ) in low-density lipoproteins .
NA
{ "id": "C0003593", "name": "apob", "pos": [ 0, 4 ] }
{ "id": "C0205666", "name": "apob-100", "pos": [ 126, 134 ] }
when analyzed after 1-2 days , satellite bands smaller than apob-100 were observed , and a very faint band with mr 200,000 appeared , which comigrated with intestinal apob ( apob-48 ) .
NA
{ "id": "C0205666", "name": "apob-100", "pos": [ 60, 68 ] }
{ "id": "C0003593", "name": "apob", "pos": [ 174, 178 ] }
when analyzed after 1-2 days , satellite bands smaller than apob-100 were observed , and a very faint band with mr 200,000 appeared , which comigrated with intestinal apob ( apob-48 ) .
NA
{ "id": "C0003593", "name": "apob", "pos": [ 60, 64 ] }
{ "id": "C0103839", "name": "apob-48", "pos": [ 174, 181 ] }
although the stop codon has been discovered recently in intestinal apob mrna , which explains the mechanism for direct synthesis of apob-48 , apparently apob-100 is also synthesized in the intestine of all eight subjects studied here , and the apob-100 degrades to a form which is apob-48-like .
NA
{ "id": "C0003593", "name": "apob", "pos": [ 67, 71 ] }
{ "id": "C0205666", "name": "apob-100", "pos": [ 244, 252 ] }
although the stop codon has been discovered recently in intestinal apob mrna , which explains the mechanism for direct synthesis of apob-48 , apparently apob-100 is also synthesized in the intestine of all eight subjects studied here , and the apob-100 degrades to a form which is apob-48-like .
NA
{ "id": "C0003593", "name": "apob", "pos": [ 67, 71 ] }
{ "id": "C0103839", "name": "apob-48", "pos": [ 281, 288 ] }
these observations suggest that mitogens , as typified by pdgf , can facilitate the cellular delivery of ldl cholesterol by both ldl-receptor-mediated and non-ldl-receptor-mediated mechanisms to provide exogenous cholesterol for use during cell replication .
NA
{ "id": "C0023824", "name": "ldl cholesterol", "pos": [ 105, 120 ] }
{ "id": "C0008377", "name": "cholesterol", "pos": [ 213, 224 ] }
the tgase secreted by j774 macrophages was internalized by i-cell disease fibroblasts , increasing the cellular content of tgase 10-fold after 8 h .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 74, 85 ] }
{ "id": "C0007634", "name": "cellular", "pos": [ 103, 111 ] }
the [ methyl-3h ] choline labeling of phosphocholine and of cdp-choline was twice as high in shr as in controls ;
NA
{ "id": "C0008405", "name": "choline", "pos": [ 18, 25 ] }
{ "id": "C0031716", "name": "phosphocholine", "pos": [ 38, 52 ] }
the [ methyl-3h ] choline labeling of phosphocholine and of cdp-choline was twice as high in shr as in controls ;
NA
{ "id": "C0008405", "name": "choline", "pos": [ 18, 25 ] }
{ "id": "C0010725", "name": "cdp-choline", "pos": [ 60, 71 ] }
the ability of various retinoids to compete with all-trans-retinoic acid for binding to crabp was assayed : 4-oxoretinoic acid and two synthetic retinoids were effective competitors , but 13-cis-retinoic acid , 3,4-didehydroretinoic acid and the acid derivative of etretinate competed poorly .
NA
{ "id": "C0035339", "name": "retinoids", "pos": [ 23, 32 ] }
{ "id": "C0022265", "name": "13-cis-retinoic acid", "pos": [ 188, 208 ] }