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a concomitant treatment of cytomegalovirus infections , such as cytomegalovirus retinitis , with phosphonoformate in patients with acquired immunodeficiency syndrome receiving 3'-azido-3'-deoxythymidine may be appropriate , and this combination may also be useful in controlling human immunodeficiency virus infection .
NA
{ "id": "C0001175", "name": "acquired", "pos": [ 131, 139 ] }
{ "id": "C0019693", "name": "human", "pos": [ 279, 284 ] }
ciprofloxacin , fleroxacin , and norfloxacin were compared with their precursor nalidixic acid and with trimethoprim in sublethal concentrations ranging from 1/32 to 1/4 of the mic .
NA
{ "id": "C0008809", "name": "ciprofloxacin", "pos": [ 0, 13 ] }
{ "id": "C0085161", "name": "fleroxacin", "pos": [ 16, 26 ] }
ds1 exhibited tolerance to other dna gyrase a subunit antagonists ( ofloxacin and ciprofloxacin ) and dna gyrase b subunit antagonists ( novobiocin and coumermycin ) but not to the aminoglycoside gentamicin , suggesting involvement of dna gyrase .
NA
{ "id": "C0002556", "name": "aminoglycoside", "pos": [ 181, 195 ] }
{ "id": "C0017436", "name": "gentamicin", "pos": [ 196, 206 ] }
in the present study , the pharmacokinetics of [ n-methyl-14c ] clarithromycin were compared with those of [ n-methyl-14c ] erythromycin in rats by radioassay and bioassay .
NA
{ "id": "C0055856", "name": "clarithromycin", "pos": [ 64, 78 ] }
{ "id": "C0014806", "name": "erythromycin", "pos": [ 124, 136 ] }
in the urine , bioactivity recovered after administration of [ 14c ] clarithromycin was sevenfold higher than that for [ 14c ] erythromycin .
NA
{ "id": "C0055856", "name": "clarithromycin", "pos": [ 69, 83 ] }
{ "id": "C0014806", "name": "erythromycin", "pos": [ 127, 139 ] }
this accounted for about 60 and 20 % of the total radioactivity in the urine for [ 14c ] clarithromycin and [ 14c ] erythromycin , respectively .
NA
{ "id": "C0055856", "name": "clarithromycin", "pos": [ 89, 103 ] }
{ "id": "C0014806", "name": "erythromycin", "pos": [ 116, 128 ] }
the mics for 78 of the isolates of the family enterobacteriaceae were greater than or equal to 64 micrograms of cefazolin , ampicillin , piperacillin , and mezlocillin per ml .
NA
{ "id": "C0002680", "name": "ampicillin", "pos": [ 124, 134 ] }
{ "id": "C0031955", "name": "piperacillin", "pos": [ 137, 149 ] }
the mics for 78 of the isolates of the family enterobacteriaceae were greater than or equal to 64 micrograms of cefazolin , ampicillin , piperacillin , and mezlocillin per ml .
NA
{ "id": "C0002680", "name": "ampicillin", "pos": [ 124, 134 ] }
{ "id": "C0025893", "name": "mezlocillin", "pos": [ 156, 167 ] }
for a description of a trauma unit and a detailed analysis of the perioperative nurse 's role in caring for the trauma patient in the or , please see the accompanying article , `` '' perioperative nursing care of the multiple trauma patient : when seconds count . '' ''
NA
{ "id": "C0043251", "name": "trauma", "pos": [ 23, 29 ] }
{ "id": "C0026771", "name": "multiple traum", "pos": [ 217, 231 ] }
treatment of rat hepatoma h-4-iie cells in culture with 2,3,7,8-tetrachlorodibenzo-p-dioxin ( tcdd ) , 2,3,7,8-tetrachlorodibenzofuran ( tcdf ) , 1,2,3,7,8-pentachlorodibenzo-p-dioxin ( pecdd ) , 1,2,3,7,8-pentachlorodibenzofuran ( pecdf ) , 1,2,7,8-tcdf , and 2,3,7-trichlorodibenzo-p-dioxin ( trcdd ) resulted in the structure-dependent induction of aryl hydrocarbon hydroxylase and ethoxyresorufin o-deethylase activities .
NA
{ "id": "C0003927", "name": "aryl hydrocarbon hydroxylase", "pos": [ 352, 380 ] }
{ "id": "C0059735", "name": "ethoxyresorufin o-deethylase", "pos": [ 385, 413 ] }
we suggest that essential lysine residues , along with divalent metal ions , are located at the oprtase active site , and form ion-pair bonds with anionic pribpp and orotate as these substrates bind to the enzyme .
NA
{ "id": "C0022023", "name": "ion", "pos": [ 70, 73 ] }
{ "id": "C0003075", "name": "anionic", "pos": [ 147, 154 ] }
an in vitro expression plasmid ( pgrap ) that contained the cdna coding for the rat mitochondrial aldehyde dehydrogenase precursor was constructed , mrna was synthesized then translated , and the in vitro synthesized precursor of aldehyde dehydrogenase was used in an in vitro import assay .
NA
{ "id": "C2827577", "name": "mitochondrial aldehyde dehydrogenase", "pos": [ 84, 120 ] }
{ "id": "C0001985", "name": "aldehyde", "pos": [ 230, 238 ] }
the development of a monoclonal antibody to bromodeoxyuridine ( brdurd ) , a thymidine analogue , is epoch making in the research of cell kinetics , and flow cytometry ( fcm ) combined with the antibody facilitates clinical use of information concerning cell kinetics of tumor cells .
NA
{ "id": "C0003250", "name": "monoclonal antibody", "pos": [ 21, 40 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 194, 202 ] }
the concentration of fk 973 required to inhibit cell growth by 50 % was 0.06-5 micrograms/ml , after 2-day exposure of this drug against human glioblastoma ( ons-6 , 12 , 23 , and ons-12/acnu ) , human medulloblastoma ( ons-76 , 81 ) , human neuroblastoma ( st ) , and murine glioblastoma ( rsv-m glioma ) .
NA
{ "id": "C0025149", "name": "medulloblastoma", "pos": [ 202, 217 ] }
{ "id": "C0017638", "name": "glioma", "pos": [ 297, 303 ] }
deficits occurred for most major causes of death , including infectious and parasitic diseases , digestive and urinary systems , and accidents .
NA
{ "id": "C0009450", "name": "infectious", "pos": [ 61, 71 ] }
{ "id": "C0030499", "name": "parasitic", "pos": [ 76, 85 ] }
cytogenetic activity of 4'-epidoxorubicin was comparable to that of doxorubicin ( adriablastin ) widely used in medical practice .
NA
{ "id": "C0014582", "name": "epidoxorubicin", "pos": [ 27, 41 ] }
{ "id": "C0013089", "name": "doxorubicin", "pos": [ 68, 79 ] }
it was shown that in both the media some amino acids such as serine , threonine , glutaminic and asparaginic acids were actively utilized by the strains mainly during the culture intensive growth while others such as glycine , alanine and tyrosine were actively utilized during the enzyme biosynthesis .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 41, 52 ] }
{ "id": "C0017890", "name": "glycine", "pos": [ 217, 224 ] }
it was shown that in both the media some amino acids such as serine , threonine , glutaminic and asparaginic acids were actively utilized by the strains mainly during the culture intensive growth while others such as glycine , alanine and tyrosine were actively utilized during the enzyme biosynthesis .
NA
{ "id": "C0002520", "name": "amino acids", "pos": [ 41, 52 ] }
{ "id": "C0001898", "name": "alanine", "pos": [ 227, 234 ] }
in vivo toxicity of rnaase from b. intermedius was 3-5 times higher than that of pancreatic rnaase .
NA
{ "id": "C0014241", "name": "rnaase", "pos": [ 20, 26 ] }
{ "id": "C0035541", "name": "pancreatic rnaase", "pos": [ 81, 98 ] }
it was shown with the method of serial dilutions on solid media that 91.7 , 87.8 , 85 and 81 per cent of the isolates were sensitive to benzylpenicillin , ampicillin , lincomycin and cefuroxime , respectively .
NA
{ "id": "C0030827", "name": "benzylpenicillin", "pos": [ 136, 152 ] }
{ "id": "C0002680", "name": "ampicillin", "pos": [ 155, 165 ] }
of the three basic amino acids , lysine shows the lowest labelling at all four measuring points .
NA
{ "id": "C0301714", "name": "basic amino", "pos": [ 13, 24 ] }
{ "id": "C0024337", "name": "lysine", "pos": [ 33, 39 ] }
although certain mcabs , when used as detecting antibody in the liquid phase , did not react with some of these antigens , they were able to bind to them when used as the capturing antibody on the solid phase .
NA
{ "id": "C0003250", "name": "mcabs", "pos": [ 17, 22 ] }
{ "id": "C0003241", "name": "antibody", "pos": [ 181, 189 ] }
neither an inactive phorbol ester , 4-alpha-phorbol-12 , 13-didecanoate , nor dimethyl sulfoxide ( dmso ) used as a solvent for pma had any effect .
NA
{ "id": "C0031586", "name": "phorbol ester", "pos": [ 20, 33 ] }
{ "id": "C0039654", "name": "pma", "pos": [ 128, 131 ] }
to determine the mechanism by which substance p ( sp ) increases vascular permeability , we examined the potency of sp and sp fragments in inducing plasma extravasation in guinea pig skin , the effects of a histamine h1 antagonist and a muscarinic antagonist on the response , the effects of sp antagonists on the response , and the specificity of tachykinin-induced tachyphylaxis of the response .
NA
{ "id": "C0038585", "name": "sp", "pos": [ 50, 52 ] }
{ "id": "C0039241", "name": "tachykinin", "pos": [ 348, 358 ] }
we compared the endothelial protection offered by 1 % hyaluronate sodium ( healon ) , 3 % hyaluronate sodium and 4 % chondroitin sulfate ( viscoat ) , and a nonviscous irrigating solution ( bss plus ) during phacoemulsification with and without traumatic intraocular lens implantation .
NA
{ "id": "C0020196", "name": "hyaluronate", "pos": [ 54, 65 ] }
{ "id": "C0087000", "name": "hyaluronate sodium", "pos": [ 90, 108 ] }
the latent period until fibroblast outgrowth began from the conjunctival explant was determined , as were the plating efficiency and doubling time of cells from first-passage cultures .
NA
{ "id": "C0016030", "name": "fibroblast", "pos": [ 24, 34 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 150, 155 ] }
wiping the goldmann tonometer tip with an isopropyl alcohol swab and then allowing the alcohol to evaporate provides a ready and efficient means of inactivating these three enveloped viruses .
NA
{ "id": "C0022237", "name": "isopropyl", "pos": [ 42, 51 ] }
{ "id": "C0001962", "name": "alcohol", "pos": [ 87, 94 ] }
incorporation of 14c-proline into proline and into hydroxyproline was taken as a measure of protein and collagen synthesis .
NA
{ "id": "C0033382", "name": "proline", "pos": [ 21, 28 ] }
{ "id": "C0020388", "name": "hydroxyproline", "pos": [ 51, 65 ] }
in contrast , canine beta-very low density lipoproteins ( beta-vldl ) , which contain apo e , bound to these cells with an affinity similar to that for fibroblasts of normal new zealand white rabbits ( kd approximately 0.6 micrograms/ml ) , although the maximal binding was only approximately 9 % of that of new zealand white cells .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 152, 163 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 326, 331 ] }
the uptake of beta-vldl by whhl cells was mediated by the ldl receptor , since preincubation of the fibroblasts with cholesterol plus 25-hydroxycholesterol inhibited beta-vldl degradation , and specific antibodies to rabbit ldl receptors abolished the degradation of beta-vldl in whhl fibroblasts .
NA
{ "id": "C0007634", "name": "cells", "pos": [ 32, 37 ] }
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 285, 296 ] }
six-month-old , mfo-fed , female whhl rabbits had significantly lower plasma concentrations of total cholesterol ( 582 +/- 20 mg/dl vs. 856 +/- 44 mg/dl , control , p less than 0.05 ) and triglycerides ( 266 +/- 21 mg/dl vs. 459 +/- 15 mg/dl , control , p less than 0.05 ) , with lower serum/plasma lipoprotein concentrations [ very low density lipoprotein ( vldl ) , ldl , high density lipoprotein ( hdl ) ] compared to control female whhl rabbits .
NA
{ "id": "C0023820", "name": "lipoprotein", "pos": [ 299, 310 ] }
{ "id": "C0023825", "name": "very low density lipoprotein", "pos": [ 328, 356 ] }
in the simultaneous administration of meprobamate and ethanol to rabbits , the blood meprobamate concentration ( bmc ) increased greatly when the maximum blood ethanol concentration ( becmax ) exceeded 1.0 mg/ml .
NA
{ "id": "C0001962", "name": "ethanol", "pos": [ 54, 61 ] }
{ "id": "C0684262", "name": "blood", "pos": [ 154, 159 ] }
thus , we subjected the rabbits to continuous infusion of ethanol so as to make the blood ethanol concentration ( bec ) constant and administered meprobamate by intravenous injection .
NA
{ "id": "C0001962", "name": "ethanol", "pos": [ 58, 65 ] }
{ "id": "C0684262", "name": "blood ethanol", "pos": [ 84, 97 ] }
everyone who cares for families with young children will be familiar with the pale sleepless look on a mother 's face , the bags under her eyes and the extra rouge worn to camouflage her fatigue if she is trying to cope with a child with a sleep disorder .
NA
{ "id": "C0728836", "name": "young children", "pos": [ 37, 51 ] }
{ "id": "C0008059", "name": "child", "pos": [ 227, 232 ] }
although the fingerprints generated from these viral rnas exhibited similar distribution patterns of non-unique rna fragments , all could be distinguished from one another on the basis of the migration of unique rna fragments .
NA
{ "id": "C0035736", "name": "viral rnas", "pos": [ 47, 57 ] }
{ "id": "C0035668", "name": "rna", "pos": [ 212, 215 ] }
the combination of the light and temperature regime did not yield any significant differences for the carcass weight at conventional temperatures and for the body weight .
NA
{ "id": "C0005911", "name": "differences", "pos": [ 82, 93 ] }
{ "id": "C0005910", "name": "body weight", "pos": [ 158, 169 ] }
subsequently , purification to an apparently homogeneous state was achieved by chromatography on deae-cellulose , sepharose cl-6b and hydroxylapatite cellulose .
NA
{ "id": "C0011050", "name": "deae-cellulose", "pos": [ 97, 111 ] }
{ "id": "C0007648", "name": "cellulose", "pos": [ 150, 159 ] }
a single dose of 7.5 mg/100 g b.wt . metyrapone , intraperitoneally , decreased the concentration of reduced ( gsh ) and oxidized ( gssg ) glutathione in the liver and kidney of female wistar rats for approximately 4-5 h .
NA
{ "id": "C0061516", "name": "gssg", "pos": [ 132, 136 ] }
{ "id": "C0017817", "name": "glutathione", "pos": [ 139, 150 ] }
the ratio between gssg and gsh was not affected in the kidney but is decreased in the liver .
NA
{ "id": "C0061516", "name": "gssg", "pos": [ 18, 22 ] }
{ "id": "C0017817", "name": "gsh", "pos": [ 27, 30 ] }
endothelin 1 , endothelin 3 and sarafotoxin all inhibited [ 125i ] -endothelin binding with ic50 's in the 100-300 pm range , whereas unrelated peptides , namely angiotensin ii , atrial natriuretic peptide , and platelet-derived growth factor failed to compete for [ 125i ] -endothelin binding .
NA
{ "id": "C0079281", "name": "endothelin 1", "pos": [ 0, 12 ] }
{ "id": "C0079284", "name": "endothelin", "pos": [ 275, 285 ] }
endothelin 1 , endothelin 3 and sarafotoxin all inhibited [ 125i ] -endothelin binding with ic50 's in the 100-300 pm range , whereas unrelated peptides , namely angiotensin ii , atrial natriuretic peptide , and platelet-derived growth factor failed to compete for [ 125i ] -endothelin binding .
NA
{ "id": "C0079283", "name": "endothelin 3", "pos": [ 15, 27 ] }
{ "id": "C0079284", "name": "endothelin", "pos": [ 275, 285 ] }
a pretreatment with alkaline phosphatase reduced the ability of pr to act as a substrate while the steroid occupancy of pr appeared to enhance the phosphorylation of the 114 kda peptide .
NA
{ "id": "C0034833", "name": "pr", "pos": [ 2, 4 ] }
{ "id": "C0034840", "name": "steroid", "pos": [ 99, 106 ] }
however , the xanthate exerted an inhibitory effect on the tpa-stimulated liberation of [ 3h ] phosphorylcholine from [ 3h ] phosphatidylcholine in cells prelabeled with [ 3h ] choline .
NA
{ "id": "C0031716", "name": "phosphorylcholine", "pos": [ 95, 112 ] }
{ "id": "C0008405", "name": "choline", "pos": [ 177, 184 ] }
the molecular weight , antibody crossreactivity , glycosidase sensitivity and lectin binding show that this protein is a normal kidney analogue of the p-glycoprotein induced in multidrug resistant cell lines .
NA
{ "id": "C0023206", "name": "lectin", "pos": [ 78, 84 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 158, 165 ] }
bovine brain smg p25a , a guanine nucleotide-binding protein with a mr of about 25,000 , bound specifically gtp , guanosine 5'- ( 3-o-thio ) triphosphate ( gtp gamma s ) and gdp .
NA
{ "id": "C0018353", "name": "gtp", "pos": [ 108, 111 ] }
{ "id": "C0079469", "name": "gtp gamma", "pos": [ 156, 165 ] }
we investigated the molecular forms of endothelin ( et ) related peptides in porcine spinal cord by high performance liquid chromatography coupled with radioimmunoassays using three antisera raised against et-1 and c-terminal fragments of et-1 and big et-1 .
NA
{ "id": "C0079284", "name": "endothelin", "pos": [ 39, 49 ] }
{ "id": "C0079281", "name": "et-1", "pos": [ 252, 256 ] }
although it is not known whether nf i functions as a protein kinase in addition to recognizing a specific sequence on dna , it is concluded that some of the eukaryotic dna binding proteins and protein kinases may belong to a gene family , various members of which are evolutionarily related and responsible for the regulation of metabolism in diverse compartments of the cell .
NA
{ "id": "C0069116", "name": "nf i", "pos": [ 33, 37 ] }
{ "id": "C0012940", "name": "dna binding proteins", "pos": [ 168, 188 ] }
the dihydropyridine ca2+-channel antagonist nifedipine at 10 microm inhibited the [ ca2+ ] i response to pdgf by 41 % in both the presence of and in the absence of external ca2+ .
NA
{ "id": "C0012315", "name": "dihydropyridine", "pos": [ 4, 19 ] }
{ "id": "C0028066", "name": "nifedipine", "pos": [ 44, 54 ] }
a substrate specificity of the kinase activity towards specific nuclear proteins is indicated by its phosphorylation of high mobility group ( hmg ) proteins isolated from endosperm and its lack of accepting histones as protein substrates .
NA
{ "id": "C0028589", "name": "nuclear proteins", "pos": [ 64, 80 ] }
{ "id": "C0019652", "name": "histones", "pos": [ 207, 215 ] }
a substrate specificity of the kinase activity towards specific nuclear proteins is indicated by its phosphorylation of high mobility group ( hmg ) proteins isolated from endosperm and its lack of accepting histones as protein substrates .
NA
{ "id": "C0028589", "name": "nuclear proteins", "pos": [ 64, 80 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 219, 226 ] }
compound iii ( oxyperoxidase ) of lignin peroxidase isozyme h8 ( pi = 3.5 ) is formed by either reduction of native ferric enzyme ( to ferrous ) followed by the reaction with dioxygen or by the addition of excess hydrogen peroxide to resting enzyme .
NA
{ "id": "C0022173", "name": "isozyme", "pos": [ 52, 59 ] }
{ "id": "C0014442", "name": "enzyme", "pos": [ 242, 248 ] }
these results suggest that the conjugation of ubiquitin to ubiquitin during polyubiquitin synthesis involves a specific conjugation system that recognizes ubiquitin and some of its derivatives , but not general proteolysis substrates , as ubiquitin acceptors .
NA
{ "id": "C0084133", "name": "polyubiquitin", "pos": [ 76, 89 ] }
{ "id": "C0041538", "name": "ubiquitin", "pos": [ 239, 248 ] }
cholesterol esterification was examined in cultured skin fibroblasts from patients with i-cell disease and pseudo-hurler polydystrophy by incubating cells pretreated without fetal calf serum for 48h , with ( 14c ) cholesterol for 24h .
NA
{ "id": "C0016030", "name": "fibroblasts", "pos": [ 57, 68 ] }
{ "id": "C0007634", "name": "cells", "pos": [ 149, 154 ] }
depression of the enzymatic activity is caused by a novel prolyendopeptidase inhibitor , whose amino acid composition is 7 glu , 1 ser , 2 gly , 1 ala , 2 pro , and 1 val , generated by inflammation .
NA
{ "id": "C0002520", "name": "amino acid", "pos": [ 95, 105 ] }
{ "id": "C0017890", "name": "gly", "pos": [ 139, 142 ] }
without mtx pretreatment , 59 % of the total incorporation of fura into nucleic acids was into dna when fura was administered .
NA
{ "id": "C0028606", "name": "nucleic acids", "pos": [ 72, 85 ] }
{ "id": "C0012854", "name": "dna", "pos": [ 95, 98 ] }
10 ( -4 ) m , damaged myocardial cells as reflected in a significant ( p less than 0.05 ) reduction in cellular protein and a further reduction in labelled choline in phosphocholine or phospholipid after adjusting for the lower protein concentrations .
NA
{ "id": "C0008405", "name": "choline", "pos": [ 156, 163 ] }
{ "id": "C0031716", "name": "phosphocholine", "pos": [ 167, 181 ] }
anoxia , for 24 hr , produced a significant ( p less than 0.05 ) reduction in labelled choline in phosphocholine without a significant change in incorporation of label in phospholipid or cellular protein .
NA
{ "id": "C0008405", "name": "choline", "pos": [ 87, 94 ] }
{ "id": "C0031716", "name": "phosphocholine", "pos": [ 98, 112 ] }
mn2+ ion forms 2-3 complexes with each conformer ( the cation being differently coordinated ) by substituting phosphoryl oxygens or n7 atoms of adenine for two water molecules in the hydration shell of the cation .
NA
{ "id": "C0022023", "name": "ion", "pos": [ 5, 8 ] }
{ "id": "C0007447", "name": "cation", "pos": [ 206, 212 ] }
the better discriminating lipoproteins and apolipoproteins in mi patients in descending order were hdl2-cholesterol greater than apo b greater than apo a-i greater than vldl-triglyceride greater than hdl-cholesterol greater than ldl/hdl2-cholesterol greater than triglycerides .
NA
{ "id": "C0003591", "name": "apolipoproteins", "pos": [ 43, 58 ] }
{ "id": "C0003593", "name": "apo b", "pos": [ 129, 134 ] }
responsiveness of the calcified tissue to norepinephrine , epinephrine , serotonin , prostaglandin f2 alpha was found to be 11-66 % when compared to that of the control .
NA
{ "id": "C0028351", "name": "norepinephrine", "pos": [ 42, 56 ] }
{ "id": "C0014563", "name": "epinephrine", "pos": [ 59, 70 ] }
analyses of the cellular cholesterol ester content by gas-liquid chromatography and esterification of [ 14c ] cholesterol indicated the conversion of crystalline cholesterol to cholesterol esters .
NA
{ "id": "C0008377", "name": "cholesterol", "pos": [ 25, 36 ] }
{ "id": "C0008387", "name": "cholesterol esters", "pos": [ 177, 195 ] }
the association of restriction fragment length polymorphism genetic markers at the apolipoprotein ai-ciii-aiv gene locus with lipid and lipoprotein levels was evaluated in subsets of the 666 caucasian students from the junior high school ( 11-14 years old ) population of muscatine , iowa .
NA
{ "id": "C0023779", "name": "lipid", "pos": [ 126, 131 ] }
{ "id": "C0023820", "name": "lipoprotein", "pos": [ 136, 147 ] }
by the simultaneous use of [ 3h ] cholesterol and [ 14c ] cholesterol it was shown that only 10-20 % of this disappearance can be explained by cholesteryl ester hydrolysis in the arterial wall .
NA
{ "id": "C0008377", "name": "cholesterol", "pos": [ 34, 45 ] }
{ "id": "C0008387", "name": "cholesteryl", "pos": [ 143, 154 ] }
clinical features in 9 cases with progressive myelopathy were not different from those previously described in tropical spastic paraparesis associated with htlv-i infection .
NA
{ "id": "C0030481", "name": "tropical spastic paraparesis", "pos": [ 111, 139 ] }
{ "id": "C0020097", "name": "htlv-i infection", "pos": [ 156, 172 ] }
isotype evaluation of the antinuclear and related antibodies in the sigad group showed a greater tendency towards igg .
NA
{ "id": "C0021017", "name": "isotype", "pos": [ 0, 7 ] }
{ "id": "C0003241", "name": "antibodies", "pos": [ 50, 60 ] }
a complete model of the human leucocyte antigen molecule ( hla-a2 ) reconstructed from the alpha-carbon coordinates was used to investigate the putative organization of a tcr/peptide/hla-a2 complex .
NA
{ "id": "C0019728", "name": "a", "pos": [ 0, 1 ] }
{ "id": "C0019733", "name": "hla-a2", "pos": [ 183, 189 ] }
differentiation antigens can be simply defined as cell surface antigens that distinguish one cell type from another .
NA
{ "id": "C0003324", "name": "differentiation antigens", "pos": [ 0, 24 ] }
{ "id": "C0003339", "name": "cell surface antigens", "pos": [ 50, 71 ] }
the ly-6 family of cell surface antigens might easily be regarded as a prototype for the analysis of the function of differentiation antigens on immunocompetent cells .
NA
{ "id": "C0003334", "name": "ly", "pos": [ 4, 6 ] }
{ "id": "C0003324", "name": "differentiation antigens", "pos": [ 117, 141 ] }
the gastrointestinal hormone , gastric inhibitory polypeptide ( gip ) , has been isolated and characterized because of its enterogastrone-type effects .
NA
{ "id": "C0017182", "name": "gastrointestinal hormone", "pos": [ 4, 28 ] }
{ "id": "C0017132", "name": "gastric inhibitory polypeptide", "pos": [ 31, 61 ] }
being the prachtl 's neurological sieve a systematic condense analysis of neurologic screening which allows to establish a normal or suspicious neurologic condition at a sensibility of 0.85 , and specificity of 0.77 with a predictive value of 0.78 ( in our experience ) , we take it as an axis variable capable to discriminate among the population with and without risk of neurologic injury or sequela .
NA
{ "id": "C0027765", "name": "neurologic", "pos": [ 21, 31 ] }
{ "id": "C0751792", "name": "of neurologic injury", "pos": [ 370, 390 ] }
the change in electrostatical potential-profile across the phospholipid membranes caused by the binding or sorption of ionic compounds ( metal cations , ionic surfactants , lipid-soluble ions , and ionophore-metal complexes ) can be estimated by the direct measuring method ( combination of zeta potential of lipid vesicles and surface potential of lipid monolayer ) .
NA
{ "id": "C0007447", "name": "cations", "pos": [ 143, 150 ] }
{ "id": "C0022023", "name": "ions", "pos": [ 187, 191 ] }
variables significantly associated with hiv infection in the partners included advanced clinical stage ( aids-related complex or aids ) , a low concentration of t4 lymphocytes and african nationality of the index case , young age of the partner , and more than 50 instances of sexual contact with the index cases .
NA
{ "id": "C0019693", "name": "hiv infection", "pos": [ 40, 53 ] }
{ "id": "C0001857", "name": "aids-related complex", "pos": [ 105, 125 ] }
variables significantly associated with hiv infection in the partners included advanced clinical stage ( aids-related complex or aids ) , a low concentration of t4 lymphocytes and african nationality of the index case , young age of the partner , and more than 50 instances of sexual contact with the index cases .
NA
{ "id": "C0019693", "name": "hiv infection", "pos": [ 40, 53 ] }
{ "id": "C0001175", "name": "aids", "pos": [ 129, 133 ] }
in order to clarify whether hiv-1 core and env antigens are destroyed during pepsin treatment , used previously for detecting hiv-1 core and env antibodies hidden in circulating immune complexes , purified recombinant env and core antigen preparations were treated with pepsin .
NA
{ "id": "C0003241", "name": "antibodies", "pos": [ 145, 155 ] }
{ "id": "C0003313", "name": "circulating immune complexes", "pos": [ 166, 194 ] }
eighteen infants born to anti-hiv-positive mothers were tested bimonthly for immunoglobulin m ( igm ) anti-hiv by western blot and hiv p24 antigen ( ag ) by enzyme-linked immunosorbent assay ( elisa ) in order to determine the role of these markers in the early diagnosis of hiv infection .
NA
{ "id": "C0019699", "name": "hiv-positive", "pos": [ 30, 42 ] }
{ "id": "C0019693", "name": "hiv infection", "pos": [ 275, 288 ] }
the inhibition of these enzymes at 0.1 % triton x-100 was present over an eightfold range of membrane protein in the assay indicating an independence of the protein/detergent ratio .
NA
{ "id": "C0025252", "name": "membrane protein", "pos": [ 93, 109 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 157, 164 ] }
these results demonstrate that cerulenin blocks aldehyde synthesis by covalent reaction with the acyl-coa reductase and indicate that the reaction may occur at a cysteine residue involved in the formation of the acyl-reductase intermediate .
NA
{ "id": "C0050688", "name": "acyl-coa reductase", "pos": [ 97, 115 ] }
{ "id": "C0085744", "name": "acyl-reductase", "pos": [ 212, 226 ] }
the o-polysaccharide fraction obtained by mild acetic acid hydrolysis of the s. madelia lipopolysaccharide was analyzed by chemical composition , nitrous acid deamination , periodate oxidation , methylation , and 1h and 13c nuclear magnetic resonance methods and was demonstrated to be composed of three polysaccharides , ps ( i ) , ps ( ii ) , and ps ( iii ) , which had the structures of repeating oligosaccharide units : ( formula ; see text )
NA
{ "id": "C0032594", "name": "polysaccharides", "pos": [ 304, 319 ] }
{ "id": "C0028959", "name": "oligosaccharide", "pos": [ 400, 415 ] }
the expression of fine and intermediate filaments in 10 cases of leiomyosarcoma originating from a large vein , 9 cases of vascular leiomyoma ( angiomyoma ) and one case of a leiomyoma originating from the wall of the saphena magna vein was studied immunohistochemically by using 6 different anti-desmin antibodies , one anti-vimentin antibody and 2 antibodies to muscle-specific isoforms of actin .
NA
{ "id": "C0206653", "name": "angiomyoma", "pos": [ 144, 154 ] }
{ "id": "C0023267", "name": "leiomyoma", "pos": [ 175, 184 ] }
blood samples analysed by high performance liquid chromatography for zeranol , its precursor zearalenone , and its main metabolites did not show any of these compounds above the laboratory limit of detection .
NA
{ "id": "C0043465", "name": "zeranol", "pos": [ 69, 76 ] }
{ "id": "C0043454", "name": "zearalenone", "pos": [ 93, 104 ] }
one patient was delivered by caesarean section because of twin pregnancy with malpresentation , and two pregnancies are still ongoing beyond the first trimester .
NA
{ "id": "C0032961", "name": "pregnancies", "pos": [ 104, 115 ] }
{ "id": "C0032979", "name": "first", "pos": [ 145, 150 ] }
the excretion of 5-oxoproline/creatinine ( mumol/mmol ) rose progressively as pregnancy advanced ( non-pregnant mean 10 , sd 5 , first trimester mean 46 , sd 12 , second trimester mean 150 , sd 180 , and third trimester mean 280 , sd 320 ) and by the third trimester was over 20 times greater than in non-pregnant women .
NA
{ "id": "C0032961", "name": "pregnancy", "pos": [ 78, 87 ] }
{ "id": "C0032979", "name": "first trimester", "pos": [ 129, 144 ] }
the excretion of 5-oxoproline/creatinine ( mumol/mmol ) rose progressively as pregnancy advanced ( non-pregnant mean 10 , sd 5 , first trimester mean 46 , sd 12 , second trimester mean 150 , sd 180 , and third trimester mean 280 , sd 320 ) and by the third trimester was over 20 times greater than in non-pregnant women .
NA
{ "id": "C0032961", "name": "pregnancy", "pos": [ 78, 87 ] }
{ "id": "C0032980", "name": "second trimester", "pos": [ 163, 179 ] }
the excretion of 5-oxoproline/creatinine ( mumol/mmol ) rose progressively as pregnancy advanced ( non-pregnant mean 10 , sd 5 , first trimester mean 46 , sd 12 , second trimester mean 150 , sd 180 , and third trimester mean 280 , sd 320 ) and by the third trimester was over 20 times greater than in non-pregnant women .
NA
{ "id": "C0032961", "name": "pregnancy", "pos": [ 78, 87 ] }
{ "id": "C0032981", "name": "third trimester", "pos": [ 251, 266 ] }
in common with many other mitochondrial proteins encoded in nuclear genes , the deduced protein sequence has an n-terminal extension that is absent from the mature protein .
NA
{ "id": "C0949610", "name": "mitochondrial proteins", "pos": [ 26, 48 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 164, 171 ] }
the location and sequence of alpha-helices in mammalian cytochromes p450 were predicted from their homology with those of cytochrome p450cam , and these sequences were generally confirmed as helical in nature by using a secondary structure prediction method .
NA
{ "id": "C0010762", "name": "cytochromes", "pos": [ 56, 67 ] }
{ "id": "C0054563", "name": "p450cam", "pos": [ 133, 140 ] }
other than these differences the model of mammalian cytochromes p450 proposed maintains the general structure of cytochrome p450cam as determined by its alpha-helical composition .
NA
{ "id": "C0010762", "name": "cytochromes p450", "pos": [ 52, 68 ] }
{ "id": "C0054563", "name": "p450cam", "pos": [ 124, 131 ] }
hence in the asp and glu mutant proteins , both reduction and cyanide binding are accompanied by proton uptake by the protein .
NA
{ "id": "C1564139", "name": "mutant proteins", "pos": [ 25, 40 ] }
{ "id": "C0033684", "name": "protein", "pos": [ 118, 125 ] }
the divalent cations magnesium , calcium , copper , strontium , and barium were much less effective , although all cations precipitate chromatin if their concentration is increased .
NA
{ "id": "C0007448", "name": "divalent cations", "pos": [ 4, 20 ] }
{ "id": "C0007447", "name": "cations", "pos": [ 115, 122 ] }
the results show that all three inhibitors form complexes with both isozymes that have 2:1 inhibitor/enzyme stoichiometry .
NA
{ "id": "C0022173", "name": "isozymes", "pos": [ 68, 76 ] }
{ "id": "C0014442", "name": "enzyme", "pos": [ 101, 107 ] }
the 3h-methylated calmodulin product was purified by reverse-phase hplc and digested with various proteases including trypsin , chymotrypsin , endoproteinase lys-c , clostripain , and staphylococcus aureus v8 protease , and the resulting peptides were separated by reverse-phase hplc .
NA
{ "id": "C0030940", "name": "proteases", "pos": [ 98, 107 ] }
{ "id": "C0030946", "name": "protease", "pos": [ 209, 217 ] }
the effect of the protein environment on the reaction sequence and the relative rates of two two-step reactions involved in the biosynthesis of complex glycans in glycoproteins has been explored by comparing the processing of biotinylated substrates either free or bound to avidin .
NA
{ "id": "C0017968", "name": "glycoproteins", "pos": [ 163, 176 ] }
{ "id": "C0004434", "name": "avidin", "pos": [ 274, 280 ] }
the incorporation of eight 14c-labeled fatty acids into human neutrophil phospholipids was investigated and the results were expressed as percent of the total phospholipid associated 14c-labeled substrate incorporated after an initial 15 min labeling and a subsequent 2 h reincubation in fatty acid-free buffer .
NA
{ "id": "C0015684", "name": "fatty acids", "pos": [ 39, 50 ] }
{ "id": "C0015688", "name": "fatty acid-free", "pos": [ 288, 303 ] }
neuroblastoma and hybrid cells had almost identical phospholipid and phosphoinositide compositions and similar activities for the enzymes metabolizing polyphosphoinositides ( pi kinase , pip phosphatase , pip kinase , pip2 phosphatase , pip2 phosphodiesterase ) .
NA
{ "id": "C0071655", "name": "polyphosphoinositides", "pos": [ 151, 172 ] }
{ "id": "C0070798", "name": "pip2", "pos": [ 237, 241 ] }
a quantitative analysis of fatty acid release by phospholipase a2 from the microsomal membrane revealed that only 5 nmol of free fatty acid per mg microsomal protein was released , including oleic acid and arachidonic acid , by treatment with 0.4 unit of phospholipase a2 per mg microsomal protein for 15 s at 23 degrees c .
NA
{ "id": "C0015684", "name": "fatty acid", "pos": [ 27, 37 ] }
{ "id": "C0015688", "name": "free", "pos": [ 124, 128 ] }
the results show that the three lectins are different from the soluble beta-galactoside-binding lectins studied thus far .
NA
{ "id": "C0023206", "name": "lectins", "pos": [ 32, 39 ] }
{ "id": "C0607430", "name": "beta-galactoside-binding lectins", "pos": [ 71, 103 ] }
conformational transitions were detected for the pancreatic zymogen -- -- phospholipase a2 transformation and different secondary contents were observed for a toxic and a nontoxic form of the phospholipase molecule .
NA
{ "id": "C0031667", "name": "phospholipase a2", "pos": [ 74, 90 ] }
{ "id": "C0031672", "name": "a", "pos": [ 202, 203 ] }
fluorescence decay measurement of the holoenzyme revealed that the fluorescence lifetime derived from the coenzyme , pyridoxal 5'-phosphate ( plp ) , was dependent on coexisting monovalent cations , whereas that of the tryptophyl residue was not , in either the apo- or the holoenzyme preparation .
NA
{ "id": "C0009235", "name": "coenzyme", "pos": [ 106, 114 ] }
{ "id": "C0034266", "name": "pyridoxal 5", "pos": [ 117, 128 ] }
the observed incorporation of lps into ldl particles was not possibly associated with a transfer of lipids or proteins from high density lipoproteins to ldl .
NA
{ "id": "C0023810", "name": "lps", "pos": [ 30, 33 ] }
{ "id": "C0023767", "name": "a", "pos": [ 90, 91 ] }
it was found that incubation of sera obtained from normolipidemic donors with cholesterol-loaded fibroblasts results in a decrease of the proportion of all small-sized particles of the hdl3 subclass , i.e. , hdl3a , hdl3b and hdl3c as well as in an increase in the proportion of large-sized particles of the hdl2 subclass ( hdl2a and hdl2b ) due to cholesterol acceptance by hdl .
NA
{ "id": "C0008377", "name": "cholesterol", "pos": [ 78, 89 ] }
{ "id": "C0023822", "name": "cholesterol", "pos": [ 349, 360 ] }