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5375abb8e4ff3d8c4b9d6a6d8c80787ccb60c868 | older taws, or deactivation of the egpws, or | older taws, or deactivation of the egpws, or ignoring its warnings when an airport is not in its database, still leave aircraft vulnerable to possible cfit incidents. in april 2010, a polish air force tupolev tu-154m aircraft crashed near smolensk, russia, in a possible cfit accident killing all passengers and crew, in... | wikipedia |
c7c2c6a3b3cb2fabf5c0867785e55f77641b2406 | prior to the development of gpws, large passenger | prior to the development of gpws, large passenger aircraft were involved in 3.5 fatal cfit accidents per year, falling to 2 per year in the mid-1970s. a 2006 report stated that from 1974, when the u.s. faa made it a requirement for large aircraft to carry such equipment, until the time of the report, there had not been... | wikipedia |
2875d3ec6b3b56701b73a951784eaffe914fa814 | in march 2000, the u.s. faa amended operating | in march 2000, the u.s. faa amended operating rules to require that all u.s. registered turbine-powered airplanes with six or more passenger seats (exclusive of pilot and copilot seating) be equipped with an faa-approved taws. the mandate affects aircraft manufactured after march 29, 2002. | wikipedia |
b82d1078f67c1d2e9706c309adf000f6c2f7f974 | beginning in the early 1970s, a number of | beginning in the early 1970s, a number of studies examined the occurrence of cfit accidents. findings from these studies indicated that many such accidents could have been avoided if a warning device called a ground proximity warning system (gpws) had been used. as a result of these studies and recommendations from the... | wikipedia |
5d0f34b97240c679c9aebfec88b901fb062a091e | in the late 1960s, a series of controlled | in the late 1960s, a series of controlled flight into terrain (cfit) accidents took the lives of hundreds of people. a cfit accident is one where a properly functioning airplane under the control of a fully qualified and certified crew is flown into terrain, water or obstacles with no apparent awareness on the part of ... | wikipedia |
1ed34211eea7d8515838c389a350cc0f17e3b697 | a ground proximity warning system (gpws) is a | a ground proximity warning system (gpws) is a system designed to alert pilots if their aircraft is in immediate danger of flying into the ground or an obstacle. the united states federal aviation administration (faa) defines gpws as a type of terrain awareness and warning system (taws). more advanced systems, introduce... | wikipedia |
02fbc1b8db5844200efaa3bd2a290a8e77c9e3d2 | harald popp is a german electrical engineer. together | harald popp is a german electrical engineer. together with karlheinz brandenburg, ernst eberlein, heinz gerhäuser, bernhard grill, jürgen herre, he developed the widespread mp3 method for audio data compression. | wikipedia |
cd935cb6136112bb6016d1848c46e89af4e77d9b | from 1982 to 1984 popp worked on a | from 1982 to 1984 popp worked on a technology transfer project for cable fault location of the friedrich-alexander-university erlangen-nürnberg.1985 he became researcher at the fraunhofer institute for integrated circuits (iis-a) in erlangen.from 1985 to 1990 was popp development engineer for digital circuits on board-... | wikipedia |
6ae43bce834f51906dbea9e044478991127e2b95 | 1975 popp graduated with the abitur (international baccalaureate | 1975 popp graduated with the abitur (international baccalaureate diploma).popp was educated at friedrich-alexander-university (fau), nuremberg, where he studied electrical engineering from 1975 to 1981 and obtained a graduate diploma. | wikipedia |
fd5911dc905024cc2aa6a4a9dfbd2518780f0379 | harald popp (born 30 september, 1956 in erlangen) | harald popp (born 30 september, 1956 in erlangen) is a german electrical engineer. together with karlheinz brandenburg, ernst eberlein, heinz gerhäuser (former institutes director of fraunhofer iis), bernhard grill, jürgen herre (all fraunhofer iis), he developed the widespread mp3 method for audio data compression. | wikipedia |
be73f2863076f4ecbe46a0816388e129907cffab | barboso is good friends with yu hoshide, and | barboso is good friends with yu hoshide, and is known by teammates as a very sociable person. his number, 63, is testament to his nickname "sixto" which he earned in college. he is also well known for his long, curly "afro" hair which he sometimes ties in a ponytail. | wikipedia |
476c3060510474f95c9621e2674bb43de8fefe9c | barboso made his international debut in the 2010 | barboso made his international debut in the 2010 long teng cup. barboso was one of the two overage players chosen for the 2012 hassanal bolkiah trophy, where he also captained the team. in august 2013, barbaso was selected to play in a friendly match against indonesia. he made his debut coming on for carli de murga in ... | wikipedia |
64a89cbe03ab9d4d9ef13e85227c667593a0ad9c | gramicidin s or gramicidin soviet is an antibiotic | gramicidin s or gramicidin soviet is an antibiotic that is effective against some gram-positive and gram-negative bacteria as well as some fungi. it is a derivative of gramicidin, produced by the gram-positive bacterium brevibacillus brevis. gramicidin s is a cyclodecapeptide, constructed as two identical pentapeptides... | wikipedia |
58c5af4dbdaed99d5702220b08ce946b4a408ae2 | gramicidin s differs from other gramicidin types in | gramicidin s differs from other gramicidin types in that it is a cationic cyclic decapeptide and has a structure of an anti-parallel beta-sheet. gramicidin s molecule is amphiphilic, with hydrophobic amino acids (d-phe, val, leu side chains) and charged aminoacid (l-orn). it exhibits strong antibiotic activity towards ... | wikipedia |
8251b335260f52d04656ae116c616f6b462cea6e | gramicidin s was discovered by russian microbiologist georgyi | gramicidin s was discovered by russian microbiologist georgyi frantsevitch gause and his wife maria brazhnikova in 1942. within the year gramicidin s was being used in soviet military hospitals to treat infection and eventually found usage at the front lines of combat by 1946. gause was awarded the stalin prize for med... | wikipedia |
ecbd1fc8384a7ea9d24adc69292a20e5b2e23cc8 | second enzyme cluster grsb contains four modules, each | second enzyme cluster grsb contains four modules, each containing condensation (c), adenylation (a), and thiolation (t) domains and thioesterase domain (te) at the end. c domain forms a peptide bond between two amino acids, d-phe and l-pro. l-val, l-orn, and l-leu are incorporated sequentially by the next three modules... | wikipedia |
dfa2af43e0096ed8b82d49397b10670ee15c9f2c | gramicidin s biosynthetic pathway consists of two-enzyme of | gramicidin s biosynthetic pathway consists of two-enzyme of nonribosomal peptide synthases (nrpss), gramicidin s synthetase i (grsa) and gramicidin s synthetase ii (grsb), to give a product as a cyclic decapeptide. within the biosynthetic pathway, there are total of five modules that specifically recognize, activate, a... | wikipedia |
b318d2772fda53e11d1bfc0aa5c3152d7db3ecf0 | it is a derivative of gramicidin, produced by | it is a derivative of gramicidin, produced by the gram-positive bacterium brevibacillus brevis. gramicidin s is a cyclodecapeptide, constructed as two identical pentapeptides joined head to tail, formally written as cyclo (- val - orn - leu -d- phe - pro -). that is to say, it forms a ring structure composed of five di... | wikipedia |
a3976521c6dbc55fa3019fc89c6f82e53e2a4f20 | muñoz was given his first head coach job | muñoz was given his first head coach job on 22 september 2019, at segunda división b 's hércules cf. he was dismissed on 9 december, having taken 12 points from 11 games and with the side in the relegation zone. | wikipedia |
7c1b463369f9db9522b227e31b6093e5b3e48d1e | on 7 december 2017, muñoz was announced as | on 7 december 2017, muñoz was announced as chen jingang 's assistant at changchun yatai f.c. of the chinese super league. the following 5 july, he joined fran fernández 's staff at ud almería in the same capacity. | wikipedia |
d8fc3970c86d29a7ac8b7b5b120167aeae45f4f6 | returning to dépor in 2005, muñoz only featured | returning to dépor in 2005, muñoz only featured in one league match (17 minutes in a 2–1 away defeat of ca osasuna on 26 february 2006) before leaving the club. he subsequently represented amateurs rsd alcalá and cp villarrobledo, retiring with the latter at the age of 32. | wikipedia |
01fe41364c8f242674a6a5f79c07e16d0dd5b997 | in 2002, muñoz signed for deportivo de la | in 2002, muñoz signed for deportivo de la coruña and was immediately loaned to real zaragoza of the second tier. on 31 august 2004, he moved to fellow league team terrassa fc also in a temporary deal. | wikipedia |
128bf48377096609bb308bf20c7148741be1aebf | muñoz was first-choice option during the 1995–96 season, | muñoz was first-choice option during the 1995–96 season, which ended in relegation. he continued to feature regularly for alba in the following campaigns, scoring his first professional goal on 10 november 1996 in a 3–1 segunda división away victory over atlético madrid b. | wikipedia |
bba4284198f81c314e0f8112f6b9da3f7c6ca02a | born in mota del cuervo, province of cuenca, | born in mota del cuervo, province of cuenca, castile-la mancha, muñoz was an albacete balompié youth graduate. on 2 september 1995, aged 19, he made his first-team – and la liga – debut, starting in a 0–3 away loss against sporting de gijón. | wikipedia |
a87c34839eb05fad6ca6a92597fbab2d17bfbd25 | hd 290327 b is an extrasolar planet which | hd 290327 b is an extrasolar planet which orbits the g-type subgiant star hd 290327, located approximately 180 light years away in the constellation orion. this planet has at least five halves the mass of jupiter and takes 6.7 years to orbit the star at a semimajor axis of 3.35 au. however unlike most other known exopl... | wikipedia |
2632e2f60ab4f54d90b09bee7c2e2dc585cb627d | hd 290327 b (also known as hip 25191 | hd 290327 b (also known as hip 25191 b) is an extrasolar planet which orbits the g-type subgiant star hd 290327, located approximately 180 light years away in the constellation orion. this planet has at least five halves the mass of jupiter and takes 6.7 years to orbit the star at a semimajor axis of 3.35 au. however u... | wikipedia |
f2b47422aaf63bb833e4fcf6c010bbdc22edf576 | the ages of domestic cats and dogs are | the ages of domestic cats and dogs are often referred to in terms of " cat years " or " dog years ", representing a conversion to human-equivalent years. one formula for cat years is based on a cat reaching maturity in approximately 1 year, which could be seen as 16 in human terms, then adding about 4 years for every y... | wikipedia |
0ac1051e0ebd207ca26c906697b14a66b08a0036 | the concept of human-equivalent energy (h-e) assists in | the concept of human-equivalent energy (h-e) assists in understanding of energy flows in physical and biological systems by expressing energy units in human terms: it provides a “feel” for the use of a given amount of energy by expressing it in terms of the relative quantity of energy needed for human metabolism, assum... | wikipedia |
c3f0135bda31a401204300318e5382256298af76 | animal models are used to learn more about | animal models are used to learn more about a disease, its diagnosis and its treatment, with animal models predicting human toxicity in up to 71% of cases. the human equivalent dose (hed) or human equivalent concentration (hec) is the quantity of a chemical that, when administered to humans, produces an effect equal to ... | wikipedia |
c87137ea41e217effb2b0e4cc189872f7e8c1203 | the gasuden jimpu or kamikaze was a japanese | the gasuden jimpu or kamikaze was a japanese seven-cylinder air-cooled radial aircraft engine from the 1920s, 1930s and 1940s. it was the first aircraft engine produced by tokyo gas and electric, often abbreviated to gasuden, and the first production engine produced in japan. it was produced in large numbers to power t... | wikipedia |
9082248de65bafc13d46d6638a02537bc44877d3 | the jimpu 5 or hitachi tempu was a | the jimpu 5 or hitachi tempu was a 9-cylinder derivative that produced between 240 and 280 hp (179 and 209 kw). the tempu became an important engine for the company, taking 53% of production in july 1944. | wikipedia |
805d808bb8e7278a7b815a120c60dfb1a4a653f8 | initially, the engine was first known as the | initially, the engine was first known as the divine wind (神風, kamikaze), but was later better known as the encampment wind (陣 風, jimpu) as the range of engines produced by the company increased. production ran from 1928 to 1944. large numbers were delivered, primarily for training and other light aircraft. amongst the ... | wikipedia |
5f9b1d111cb5867596fce7b10a2fe22d5f3d6262 | the resulting engine was a single row radial | the resulting engine was a single row radial with seven cylinders of bore 115 mm (4.5 in) and stroke 120 mm (4.7 in). running on 80 octane fuel, the engine was rated at sea level at 150 hp (112 kw). the engine had no compressor, although some later models were fitted with a single speed mechanical supercharger to boost... | wikipedia |
2f662985b3d2c684719f54e510160ee05d92de04 | the early development of aircraft in japan was | the early development of aircraft in japan was fully dependent on engines from abroad, although many of these designs were license built by japanese companies. gasuden was no exception, building le rhone rotaries in the early 1920s. however, by 1926, the company had gained enough experience to develop its own first eng... | wikipedia |
37d8ea0c7e3b199579dbe76e9166a78bf35a8fce | the gasuden jimpu or kamikaze (later produced by | the gasuden jimpu or kamikaze (later produced by hitachi) was a japanese seven-cylinder air-cooled radial aircraft engine from the 1920s, 1930s and 1940s. it was the first aircraft engine produced by tokyo gas and electric (神風, 東京瓦斯電気工業, tokyo gasu denki), often abbreviated to gasuden, and the first production engine p... | wikipedia |
03f5dfb51e989a31b5274da10b802593f20d31dd | christian gustav wilhelm müller was a german zoologist | christian gustav wilhelm müller was a german zoologist specializing in ostracoda. in 1895 he succeeded carl eduard adolph gerstaecker as director of the zoological museum at greifswald, a position he maintained until 1923. he was the taxonomic authority of numerous taxa in ostracoda; a few examples being the subfamily ... | wikipedia |
74e2129449ed50ca5b8d0bfd350895efcb8bcf0b | he was the taxonomic authority of numerous taxa | he was the taxonomic authority of numerous taxa in ostracoda; a few examples being the subfamily conchoeciinae and the genera archiconchoecia, cytherois and stenocypria. in 1965, the genus muellerina bassiouni (family hemicytheridae) was named in his honor. | wikipedia |
f4a9261d273fd8ba8032277a8c2edcb02cb8bb6d | howard elson bigelow was an american mycologist, born | howard elson bigelow was an american mycologist, born in 1923 in greenfield, massachusetts, and died in 1987. he studied at oberlin college from 1941 to 1943. he left college to fight in the american army. he returned to oberlin after the war and obtained his bachelor of arts in 1949 and his master of arts in 1951. he ... | wikipedia |
93518de60d7d5a68aff4d0592b5b5b9cd32baf99 | howard elson bigelow (june 28, 1923 – november | howard elson bigelow (june 28, 1923 – november 21, 1987) was an american mycologist, born in 1923 in greenfield, massachusetts, and died in 1987. he studied at oberlin college from 1941 to 1943. he left college to fight in the american army. he returned to oberlin after the war and obtained his bachelor of arts in 1949... | wikipedia |
a0e1c502d31df05c8b98cac2b3138fada358a7d2 | immunolabeling is a biochemical process that enables the | immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. antigens are organic molecules, usually proteins, capable of binding to an antibody. these antigens can be visualized using a combination of antigen-specific antibody a... | wikipedia |
8760adc33e33f27db71ed7a188aa9417956f9737 | research has been conducted to test the compatibility | research has been conducted to test the compatibility of immunolabeling with fingerprints. sometimes, fingerprints are not clear enough to recognize the ridge pattern. immunolabeling may be a way for forensic personnel to narrow down who left the print. researchers conducted a study which tested the compatibility of im... | wikipedia |
9f7bbc31138ecfe735d1872f58ef98d3df5f7f6f | studies have shown that the size of the | studies have shown that the size of the gold particle must be enlarged (>40 nm) to view the cells in low magnification, but gold particles that are too large can decrease the efficiency of the binding of the gold tag. scientists have concluded the usage of smaller gold particles (1-5 nm) should be enlarged and enhanced... | wikipedia |
8fe07e3062c1026f73478051725669837b817924 | immunolabeling with gold particles, also known as immunogold | immunolabeling with gold particles, also known as immunogold staining, is used regularly with scanning electron microscopy and transmission electron microscopy to successfully identify the area within cells and tissues where antigens are located. the gold particle labeling technique was first published by faulk, w. and... | wikipedia |
8fcf05bc35b42b42486fa09315a1f93f0871388a | scanning electron microscopy is a frequently used immunolabeling | scanning electron microscopy is a frequently used immunolabeling technique. sem is able to detect the surface of cellular components in high resolution. this immunolabeling technique is very similar to the immuno-fluorescence method, but a colloidal gold tag is used instead of a fluorophore. overall, the concepts are v... | wikipedia |
898ae3b8e17522437ea80cd95206ebe0f09bba1e | scanning electron microscopy (sem) uses a scanning electron | scanning electron microscopy (sem) uses a scanning electron microscope, which produces large images that are perceived as three-dimensional when, in fact, they are not. this type of microscope concentrates a beam of electrons across a very small area (2-3 nm) of the specimen in order to produce electrons from said spec... | wikipedia |
7987350a4e03b9ddcfa81ae0233e6b7098fa6f37 | transmission electron microscopy (tem) uses a transmission electron | transmission electron microscopy (tem) uses a transmission electron microscope to form a two-dimensional image by shooting electrons through a thin piece of tissue. the brighter certain areas are on the image, the more electrons that are able to move through the specimen. transmission electron microscopy has been used ... | wikipedia |
fb652979ea96cd1cbc38dff3fdca1db2d9c3f654 | immunolabeling and electron microscopy are often used to | immunolabeling and electron microscopy are often used to view chromosomes. a study was conducted to view possible improvements of immunolabeling chromosome structures, such as topoisomerase iiα and condensin in dissected mitotic chromosomes. in particular, these investigators used uv irradiation of separated nuclei or ... | wikipedia |
d77c47a722fb7315df2938ffd1765037cac93e23 | electron microscopy is a common method that uses | electron microscopy is a common method that uses the immunolabeling technique to view tagged tissues or cells. the electron microscope method follows many of the same concepts as immunolabeling for light microscopy, where the particular antibody is able to recognize the location of the antigen of interest and then be v... | wikipedia |
75e2f3a94e5a5752ecdf8ca9465cbdbe75fecda9 | electron microscopy (em) is a focused area of | electron microscopy (em) is a focused area of science that uses the electron microscope as a tool for viewing tissues. electron microscopy has a magnification level up to 2 million times, whereas light microscopy only has a magnification up to 1000-2000 times. there are two types of electron microscopes, the transmissi... | wikipedia |
2982010cc8753e2bcb46dc4d00e6870058e4bbf2 | light microscopy is the use of a light | light microscopy is the use of a light microscope, which is an instrument that requires the usage of light to view the enlarged specimen. in general, a compound light microscope is frequently used, where two lenses, the eyepiece, and the objective work simultaneously to generate the magnification of the specimen. light... | wikipedia |
491300ef9c2390563989ed6134a3b7fd5e1f712b | in establishing the specificity of antibodies, the key | in establishing the specificity of antibodies, the key factor is the type of synthetic peptides or purified proteins being used. the lesser the specificity of the antibody, the greater the chance of visualizing something other than the target antigen. in the case of synthetic peptides, the advantage is the amino acid s... | wikipedia |
8cfa5912fe37a7c77f42bdc3eca7953612a00de0 | overall, antibodies must bind to the antigens with | overall, antibodies must bind to the antigens with a high specificity and affinity. the specificity of the binding refers to an antibody's capacity to bind and only bind a single target antigen. scientists commonly use monoclonal antibodies and polyclonal antibodies, which are composed of synthetic peptides. during the... | wikipedia |
bb30b0b2b6e8d21ec83e9b7330b7cb5284196812 | different indirect methods can be employed to achieve | different indirect methods can be employed to achieve high degrees of specificity and sensitivity. first, two-step protocols are often used to avoid the cross-reaction between the immunolabeling of multiple primary and secondary antibody mixtures, where secondary fragment antigen-binding antibodies are frequently used.... | wikipedia |
5cfe9b7c50b7161ce3f88790adc4cef6353d6d2f | there are two methods involved in immunolabeling, the | there are two methods involved in immunolabeling, the direct and the indirect methods. in the direct method of immunolabeling, the primary antibody is conjugated directly to the tag. the direct method is useful in minimizing cross-reaction, a measure of nonspecificity that is inherent in all antibodies and that is mult... | wikipedia |
60e1ac786b20a886ed916b4337dcd24492319c11 | typical tags include: a fluorescent compound, gold beads, | typical tags include: a fluorescent compound, gold beads, a particular epitope tag, or an enzyme that produces a colored compound. the association of the tags to the target via the antibodies provides for the identification and visualization of the antigen of interest in its native location in the tissue, such as the c... | wikipedia |
7fb182538e6d944f4786f8a572d55e3b77d88782 | there are two complex steps in the manufacture | there are two complex steps in the manufacture of antibody for immunolabeling. the first is producing the antibody that binds specifically to the antigen of interest and the second is fusing the tag to the antibody. since it is impractical to fuse a tag to every conceivable antigen-specific antibody, most immunolabelin... | wikipedia |
a61f73a677dfc7c6de89a5bafd4bd2014402dc5c | immunolabeling is a biochemical process that enables the | immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. antigens are organic molecules, usually proteins, capable of binding to an antibody. these antigens can be visualized using a combination of antigen-specific antibody a... | wikipedia |
95caf81570d96398928b6e225323a166e1d8390e | graham c. ousey is an american sociologist and | graham c. ousey is an american sociologist and criminologist. he is a professor of sociology at the college of william & mary, where he is also the chair of the sociology department. he is known for researching immigration and crime. | wikipedia |
95caf81570d96398928b6e225323a166e1d8390e | graham c. ousey is an american sociologist and | graham c. ousey is an american sociologist and criminologist. he is a professor of sociology at the college of william & mary, where he is also the chair of the sociology department. he is known for researching immigration and crime. | wikipedia |
45f4f75909f8ed88e0acc284be3ea4df371f3a07 | in enzymology, a glutamine-phenylpyruvate transaminase is an enzyme | in enzymology, a glutamine-phenylpyruvate transaminase is an enzyme that catalyzes the chemical reaction thus, the two substrates of this enzyme are l-glutamine and phenylpyruvate, whereas its two products are 2-oxoglutaramate and l-phenylalanine. this enzyme belongs to the family of transferases, to be specific, the t... | wikipedia |
8a7cb5a6aaecb68ab523c07ce4d983d8e07799fa | this enzyme belongs to the family of transferases, | this enzyme belongs to the family of transferases, to be specific, the transaminases, that transfer nitrogenous groups. the systematic name of this enzyme class is l-glutamine:phenylpyruvate aminotransferase. other names in common use include glutamine transaminase k, and glutamine-phenylpyruvate aminotransferase. it e... | wikipedia |
d5dfb4238612f7d63cdafd9902d53960b163971d | helen lee is a chinese-born, british–french medical researcher | helen lee is a chinese-born, british–french medical researcher who won the european inventor award 2016 in the popular prize category for inventing diagnostic kits for resource-poor regions of the globe. she is the ceo of diagnostics for the real world. she has been based at the university of cambridge since 1996. | wikipedia |
0ba05a86b45a7659ffc9a7c73d3c110079a296bb | in 2019, she was appointed as a judge | in 2019, she was appointed as a judge for the european inventor award. in may 2020, lee was recognised on the times ' science power list because her invention, the diagnostic kit samba ii, is being repurposed for use in covid-19 testing. | wikipedia |
f3f6388218b4b4f0c7af9239f3f2b990b9959954 | in 2016, she was winner of the european | in 2016, she was winner of the european inventor award in the popular prize category and received more than 36300 votes according to benoît battistelli, the president of the european patent office, "the overwhelming public vote for helen lee recognises her major contribution to the early detection and treatment of infe... | wikipedia |
194dbece42368b05e56420bf53e31b95dba6bf46 | she has been associate professor as the head | she has been associate professor as the head of the diagnostics development unit at the university of cambridge, where she developed simple robust hiv tests for developing countries. the diagnostic kit, named "samba" (simple amplification-based assay) was tested in sub-saharan africa with médecins sans frontières. the ... | wikipedia |
d5dfb4238612f7d63cdafd9902d53960b163971d | helen lee is a chinese-born, british–french medical researcher | helen lee is a chinese-born, british–french medical researcher who won the european inventor award 2016 in the popular prize category for inventing diagnostic kits for resource-poor regions of the globe. she is the ceo of diagnostics for the real world. she has been based at the university of cambridge since 1996. | wikipedia |
a96e90bdd9a6859f6209d40b156f77cd8dafd85b | high-content screening (hcs), also known as high-content analysis | high-content screening (hcs), also known as high-content analysis (hca) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or rnai that alter the phenotype of a cell in a desired manner. hence high content screening is a type of phe... | wikipedia |
0e93f8218e9fc88c9413f2ff3d6535389c7b1472 | by analogy, if one imagines a football field | by analogy, if one imagines a football field and dinner plates laid across it, instead of looking at all of them, the investigator would choose a handful near the score line and had to leave the rest. in this analogy the field is a tissue culture dish, the plates the cells growing on it. while this was a reasonable and... | wikipedia |
23c4d9ba24cd64493de6edd8c1542b7b66005972 | in review, classical 20th century cell biology used | in review, classical 20th century cell biology used cell lines grown in culture where the experiments were measured using very similar to that described here, but there the investigator made the choice on what was measured and how. in the early 1990s, the development of ccd cameras (charge coupled device cameras) for r... | wikipedia |
d572b67c94787ee54b8e634d5aa48281cffbbb40 | for cell-based approaches the utility of automated cell | for cell-based approaches the utility of automated cell biology requires an examination of how automation and objective measurement can improve the experimentation and the understanding of disease. first, it removes the influence of the investigator in most, but not all, aspects of cell biology research and second it m... | wikipedia |
71f015f9db3a35d6893362a83965ab293f7a5ad2 | this technology allows a (very) large number of | this technology allows a (very) large number of experiments to be performed, allowing explorative screening. cell-based systems are mainly used in chemical genetics where large, diverse small molecule collections are systematically tested for their effect on cellular model systems. novel drugs can be found using screen... | wikipedia |
09e5ee01c2ed3938ff0755714b04d9c4839159b2 | many screens are analyzed using the image analysis | many screens are analyzed using the image analysis software that accompanies the instrument, providing a turn-key solution. third-party software alternatives are often used for particularly challenging screens or where a laboratory or facility has multiple instruments and wishes to standardize to a single analysis plat... | wikipedia |
91e910bdead9647f40dcf8a9402221633bee080b | hcs instruments on the market today can be | hcs instruments on the market today can be separated based on an array of specifications that significantly influence the instruments versatility and overall cost. these include speed, a live cell chamber that includes temperature and co control (some also have humidity control for longer term live cell imaging), a bui... | wikipedia |
2d56ca4a574604d4916fdbc150c9c9e226a5efe6 | high-content screening technology is mainly based on automated | high-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with it-systems for the analysis and storage of the data.“high-content” or visual biology technology has two purposes, first to acquire spatially or temporally resolved information on an event and second... | wikipedia |
60f973f7747396ab0563cc39476f4fc5a0f1203b | automated image based screening permits the identification of | automated image based screening permits the identification of small compounds altering cellular phenotypes and is of interest for the discovery of new pharmaceuticals and new cell biological tools for modifying cell function. the selection of molecules based on a cellular phenotype does not require a prior knowledge of... | wikipedia |
e5ce3dbe9fa149991b26556184f23e9562285751 | at a cellular level, parallel acquisition of data | at a cellular level, parallel acquisition of data on different cell properties, for example activity of signal transduction cascades and cytoskeleton integrity is the main advantage of this method in comparison to the faster but less detailed high throughput screening. while hcs is slower, the wealth of acquired data a... | wikipedia |
26242e06384ec4c4bdbeebfaa3a647e7c9411a3e | the technology may be used to determine whether | the technology may be used to determine whether a potential drug is disease modifying. for example, in humans g-protein coupled receptors (gpcrs) are a large family of around 880 cell surface proteins that transduce extra-cellular changes in the environment into a cell response, like triggering an increase in blood pre... | wikipedia |
55469011cc1351e831ab2d9b513b0645da39d124 | high-content screening (hcs) in cell-based systems uses living | high-content screening (hcs) in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells. hcs is also used to discover and optimize new drug candidates. high content screening is a combination of modern cell biology, with all its molecular tools, with a... | wikipedia |
5884363a1800e672b65d9103eb113958a08b833b | in high content screening, cells are first incubated | in high content screening, cells are first incubated with the substance and after a period of time, structures and molecular components of the cells are analyzed. the most common analysis involves labeling proteins with fluorescent tags, and finally changes in cell phenotype are measured using automated image analysis.... | wikipedia |
83b4500bc6d71913013baefcc81768d1335e80e1 | high-content screening (hcs), also known as high-content analysis | high-content screening (hcs), also known as high-content analysis (hca) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or rnai that alter the phenotype of a cell in a desired manner. hence high content screening is a type of phe... | wikipedia |
627a1219c2f58f20b1cc2888bb01e43bdc733731 | he was honoured by grants, prizes, medals, and | he was honoured by grants, prizes, medals, and memberships, to list some of them: fellow of the australian academy of science (1968), thomas ranken lyle medal (1972), member of the american optical society (1974), overseas fellow of churchill college, cambridge (1979), walter burfitt medal (roy. soc. nsw) (1980), c. e.... | wikipedia |
a6a08c9caa56d01cd7a752309200a782dfeb1590 | when einstein was still alive, as with many | when einstein was still alive, as with many other theorists hans buchdahl could not escape the lure of the famous scientist's " unified field theory " of gravitation and electricity. however, as buchdahl's papers in this field show, he was attracted by the enlarged constructive possibilities of the more general geometr... | wikipedia |
8d8d1f04b1c6447009c85168c9559a8f4725eeab | in gravitational theory, buchdahl's contribution on einstein field | in gravitational theory, buchdahl's contribution on einstein field equation or scalar-tensor theory are almost as well known as his spherically symmetric solutions describing the interior of stars. from his work on higher-order lagrangians he concluded that theories with quadratic lagrangians or f(r)-theories are unphy... | wikipedia |
6c6ab9c62341a6b37a625625f38a9a9c54cf8745 | buchdahl's interest in tensor and spinor analysis was | buchdahl's interest in tensor and spinor analysis was related to dealing with formalisms and calculational procedures, be it spherical and spheroidal harmonics. while working with weyl's theory and quadratic lagrangians, he decided to present the euler–lagrange derivative of the most general lagrangian built from the m... | wikipedia |
80d51568f00d64de95a45dcefd3dcacbe4b42455 | his first interest in thermodynamics focused on fitting | his first interest in thermodynamics focused on fitting carathéodory 's axiomatic formulation better to a physicist's intuition. buchdahl's attempt at making the foundations of thermodynamics more concise was far from advertising the use of the axiomatic method; instead it was an endeavour allowing "physical intuition ... | wikipedia |
660fef42dabe9890f2f34c56457ca943629c5f92 | when working at the waterworth hobart annexe, buchdahl | when working at the waterworth hobart annexe, buchdahl found the formulas for optical aberration coefficients taken to high orders that the waterworth group used in designing imaging systems. these formulas were later applied worldwide, including in systems carried by satellites. at the same time, he also continued res... | wikipedia |
a7a4d90f3040e79a269fe4de6db7b256543a123a | when world war ii began, the uk government, | when world war ii began, the uk government, unable to determine individual allegiance, interned german nationals including many jewish refugees already fully assimilated. in july 1940, hans came to australia together with gerd on board the hmt dunera. he was detained initially at hay in new south wales, then at the tat... | wikipedia |
92f0eebf083888809092273b7c8980b5587f6916 | hans adolf buchdahl was born in mainz, germany, | hans adolf buchdahl was born in mainz, germany, in a jewish family (he used the spelling adolph to dissociate himself from hitler). his older brother gerd buchdahl was a well-known philosopher in science. in 1933, gerd took hans with him to england, to escape the nazi government. at london, he completed a bsc and recei... | wikipedia |
cc048b94f07522003f44e31857bc18e6a8aec0e6 | hans adolf buchdahl (7 july 1919 – 7 | hans adolf buchdahl (7 july 1919 – 7 january 2010) was a german-born australian physicist. he contributed to general relativity, thermodynamics and optics. he is particularly known for developing f(r) gravity and buchdahl's theorem on the schwarzschild's solution for the inside of a spherical star. | wikipedia |
6809f15a9a4a9b01637fe33e2b030da11c208ee9 | immunogold labeling or immunogold staining (igs) is a | immunogold labeling or immunogold staining (igs) is a staining technique used in electron microscopy. this staining technique is an equivalent of the indirect immunofluorescence technique for visible light. colloidal gold particles are most often attached to secondary antibodies which are in turn attached to primary an... | wikipedia |
ec64b1d07fd6f191a4f9ca86a99d081eb327969b | thin sections are required for immunogold labeling and | thin sections are required for immunogold labeling and these can produce misleading images; a thin slice of a cell component may not give an accurate view of its three-dimensional structure. for example, a microtubule may appear as a 'spike' depending on which plane the sectioning occurred. to overcome this limitation ... | wikipedia |
baba2ebb421ca31123abdb8e4239d0a99fce1767 | an inherent limitation to the immunogold technique is | an inherent limitation to the immunogold technique is that the gold particle is around 15-30 nm away from the site to which the primary antibody is bound (when using a primary and secondary antibodies labeling strategy). the precise location of the targeted molecule can therefore not be accurately calculated. gold part... | wikipedia |
0360adcc9849afa0f3c06b5098fbf828a2429975 | although immunogold labeling is typically used for transmission | although immunogold labeling is typically used for transmission electron microscopy, when the gold is 'silver-enhanced' it can be seen using brightfield microscopy. the silver enhancement increases the particle size, also making scanning electron microscopy possible. in order to produce the silver-enhanced gold particl... | wikipedia |
4984b12bcc435efd68ddd04b1c576cbdf4c356cf | immunogold labeling can be used to visualize more | immunogold labeling can be used to visualize more than one target simultaneously. this can be achieved in electron microscopy by using two different-sized gold particles. an extension of this method used three different sized gold particles to track the localisation of regulatory peptides. a more complex method of mult... | wikipedia |
952ea2ecdf569fb92b6ba7c0d1e151bae64b25fe | the prepared sample is then incubated with a | the prepared sample is then incubated with a specific antibody designed to bind the molecule of interest. next, a secondary antibody which has gold particles attached is added, and it binds to the primary antibody. gold can also be attached to protein a or protein g instead of a secondary antibody, as these proteins bi... | wikipedia |
b7f1a71dc9395061e1dc81704e277e6945f72dd5 | immunogold labeling was first used in 1971 by | immunogold labeling was first used in 1971 by faulk and taylor to identify salmonella antigens. it was first applied in transmission electron microscopy (tem) and was especially useful in highlighting proteins found in low densities, such as some cell surface antigens. as the resolution of scanning electron microscopy ... | wikipedia |
601184b9772b0f2ba8c00768bfaf992e57060eaf | immunogold labeling or immunogold staining (igs) is a | immunogold labeling or immunogold staining (igs) is a staining technique used in electron microscopy. this staining technique is an equivalent of the indirect immunofluorescence technique for visible light. colloidal gold particles are most often attached to secondary antibodies which are in turn attached to primary an... | wikipedia |
bce547dfb39192402bcddabdc5a85ae35db79e03 | james mcalinden was an irish footballer who played | james mcalinden was an irish footballer who played as a forward for several clubs, most notably, belfast celtic, portsmouth, shamrock rovers and southend united. as an international, mcalinden also played for both ireland teams – the ifa xi and the fai xi. after retiring as a player, he went on to manage glenavon, dist... | wikipedia |
0f9c72757b04efd2b97dfb29b5c408c8749271c3 | in 1955 mcalinden became player/manager of glenavon. he | in 1955 mcalinden became player/manager of glenavon. he continued playing for a further year before finally retiring as a player to concentrate on management. during a thirteen-year spell with glenavon he guided them two irish league titles, three irish cup victories and one gold cup. after leaving glenavon, mcalinden ... | wikipedia |
54f7d9d2bc74fe289adb69a78cff9f84272d8b1c | in 1946, while with portsmouth, mcalinden also made | in 1946, while with portsmouth, mcalinden also made 2 appearances for the fai xi. he was one of several players born in northern ireland who benefited from the fai's attempts to establish their all-ireland influence. in june 1946 when the fai organised an iberian tour, mcalinden, together with jackie vernon, billy mcmi... | wikipedia |
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