id stringlengths 7 10 | title stringlengths 8 354 | content stringlengths 91 4.1k | contents stringlengths 164 4.24k | embedding listlengths 384 384 |
|---|---|---|---|---|
PMID:20855 | pH effects on ciliomotility and morphology of respiratory mucosa. | Tracheal cilia of cows exposed, in vitro, for 20 hours to different acidities and alkalinities of sulfuric acid and sodium hydroxide, respectively, showed ciliomotility at pH values as low as 4.9, while the epithelial cells began to be expelled from the mucosa at pH 6.7, initiating a reduction of ciliary function aroun... | pH effects on ciliomotility and morphology of respiratory mucosa. Tracheal cilia of cows exposed, in vitro, for 20 hours to different acidities and alkalinities of sulfuric acid and sodium hydroxide, respectively, showed ciliomotility at pH values as low as 4.9, while the epithelial cells began to be expelled from the ... | [
-0.018655642867088318,
-0.036462992429733276,
0.020669784396886826,
0.08913833647966385,
-0.016972506418824196,
-0.005096066743135452,
0.07437994331121445,
0.013561929576098919,
-0.030648333951830864,
0.023118309676647186,
-0.01760445535182953,
-0.10956066846847534,
0.002136210212484002,
0... |
PMID:20861 | Studies on the cell cycle of Myxobacter AL-1. II. Activities of seven enzymes during the cell cycle. | The properties of seven enzymes were studied in extracts from Myxobacter AL-1. The enzymes were isocitrate dehydrogenase (E.C.1.1.1.42), succinate dehydrogenase (E.C.1.3.99.1), alkaline phosphatase (E.C.3.1.3.1), alpha-glucosidase (E.C.3.2.1.20), beta-glucosidase (E.C.3.2.1.21), beta-galactosidase (E.C.3.2.1.23), and N... | Studies on the cell cycle of Myxobacter AL-1. II. Activities of seven enzymes during the cell cycle. The properties of seven enzymes were studied in extracts from Myxobacter AL-1. The enzymes were isocitrate dehydrogenase (E.C.1.1.1.42), succinate dehydrogenase (E.C.1.3.99.1), alkaline phosphatase (E.C.3.1.3.1), alpha-... | [
-0.057780519127845764,
-0.03469603508710861,
-0.03467780351638794,
0.03159341961145401,
0.011758207343518734,
-0.061142899096012115,
0.003085752483457327,
0.014988834038376808,
-0.028466613963246346,
-0.01326768845319748,
0.01137541700154543,
-0.07797059416770935,
0.024152256548404694,
-0.... |
PMID:20862 | Dependence of sulphate uptake by Anacystis nidulans on energy, on osmotic shock and on sulphate stravation. | Sulphate uptake by Anacystis nidulans under aerobic conditions in the light was found to be sensitive to metabolic poisons, such as N,N'-dicyclohexylcarbodiimide and carbonyl cyanide m-chlorophenyl hydrazone. It was also depressed by darkness. The sulphate absorption is an energy-dependent process. Sulphate uptake was ... | Dependence of sulphate uptake by Anacystis nidulans on energy, on osmotic shock and on sulphate stravation. Sulphate uptake by Anacystis nidulans under aerobic conditions in the light was found to be sensitive to metabolic poisons, such as N,N'-dicyclohexylcarbodiimide and carbonyl cyanide m-chlorophenyl hydrazone. It ... | [
-0.06918137520551682,
-0.0049237883649766445,
-0.0372132770717144,
0.012082906439900398,
0.028326770290732384,
-0.008849984034895897,
0.07511164247989655,
-0.007602139376103878,
-0.07267948240041733,
-0.019169282168149948,
0.012896247208118439,
-0.08803878724575043,
0.035412441939115524,
0... |
PMID:20863 | The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa. | The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and below this concentration the amount of HCN produced decreases sharply and at 0... | The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa. The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and b... | [
-0.062470365315675735,
0.0365041084587574,
-0.023452945053577423,
0.027416016906499863,
0.02696133404970169,
-0.023669593036174774,
0.014813636429607868,
0.03468434512615204,
-0.07766880840063095,
0.06362036615610123,
0.013637191615998745,
-0.05654454976320267,
0.048034850507974625,
0.0502... |
PMID:20864 | The role of vanadium in gree plants. II. Vanadium in green algae--two sites of action. | Cells of Chlorella pyrenoidosa, derived from vanadium free agar slants, respond with great sensitivity to microamounts of vanadium, added as NH4VO3 to autotrophic liquid cultures. Between 0.01 and 1 microgram V per litre nutrient medium (2-10(-10)-2-10(-8) g-at/1), the algae respond with a continuous incrase in dry wei... | The role of vanadium in gree plants. II. Vanadium in green algae--two sites of action. Cells of Chlorella pyrenoidosa, derived from vanadium free agar slants, respond with great sensitivity to microamounts of vanadium, added as NH4VO3 to autotrophic liquid cultures. Between 0.01 and 1 microgram V per litre nutrient med... | [
-0.04518952593207359,
0.00311778555624187,
-0.0035087631549686193,
0.033003970980644226,
0.047507937997579575,
0.002947198925539851,
-0.002271505305543542,
0.07648742198944092,
-0.09784682095050812,
0.0064717563800513744,
0.04835907369852066,
-0.09197447448968887,
0.011967185884714127,
0.0... |
PMID:20865 | Alpha-isopropylmalate synthase from Alcaligenes eutrophus H 16. III. Endproduct inhibition and its relief by valine and isoleucine. | The alpha-isopropylmalate synthase (EC 4.1.3.12) from Alcaligenes eutrophus H 16 was inhibited by L-leucine and alpha-ketoisocaproate. The extent of inhibition was influenced by substrate- and inhibitor concentrations as well as by the pH. Intermediary plateaus, which always appeared in the inhibition curves, suggested... | Alpha-isopropylmalate synthase from Alcaligenes eutrophus H 16. III. Endproduct inhibition and its relief by valine and isoleucine. The alpha-isopropylmalate synthase (EC 4.1.3.12) from Alcaligenes eutrophus H 16 was inhibited by L-leucine and alpha-ketoisocaproate. The extent of inhibition was influenced by substrate-... | [
-0.08823241293430328,
-0.01638750173151493,
-0.037923771888017654,
0.039843421429395676,
-0.03039274923503399,
-0.037852101027965546,
0.0070754908956587315,
0.039959557354450226,
-0.06138947978615761,
0.024454422295093536,
0.08331110328435898,
-0.05705680698156357,
0.04882767051458359,
-0.... |
PMID:20866 | An NADP-linked acetoacetyl CoA reductase from Zoogloea ramigera. | Zoogloea ramigera I-16 M was found to contain two stereospecific acetoacetyl CoA reductases; one was NADP+-linked and D(-)-beta-hydroxybutyryl CoA specific and the other was NAD+-linked and L(+)-isomer specific. The NADP+-linked enzyme, purified approximately 150-fold, had a pH optimum for the reduction of acetoacetyl ... | An NADP-linked acetoacetyl CoA reductase from Zoogloea ramigera. Zoogloea ramigera I-16 M was found to contain two stereospecific acetoacetyl CoA reductases; one was NADP+-linked and D(-)-beta-hydroxybutyryl CoA specific and the other was NAD+-linked and L(+)-isomer specific. The NADP+-linked enzyme, purified approxima... | [
-0.08585116267204285,
-0.002105576219037175,
-0.0542588010430336,
0.02758733555674553,
0.06407532095909119,
-0.009316951967775822,
0.05689838156104088,
-0.029636623337864876,
-0.043683070689439774,
-0.04208628088235855,
0.04311417043209076,
-0.07932941615581512,
-0.001826259889639914,
-0.0... |
PMID:20867 | Prostaglandins. A review of neurophysiology and psychiatric implications. | This article reviews the function of prostaglandins (PGs) in the nervous system and discusses the possible alterations in PG metabolism as relating to mental illness. The PGs are a unique group of cyclic fatty acids whose immediate precursors are thought to function postsynaptically by inhibition or facillitation of ne... | Prostaglandins. A review of neurophysiology and psychiatric implications. This article reviews the function of prostaglandins (PGs) in the nervous system and discusses the possible alterations in PG metabolism as relating to mental illness. The PGs are a unique group of cyclic fatty acids whose immediate precursors are... | [
-0.013830391690135002,
0.029417352750897408,
-0.0035415117163211107,
-0.005197520367801189,
-0.012278085574507713,
0.038935672491788864,
0.07938660681247711,
-0.013776510022580624,
0.0017407757695764303,
-0.03654685616493225,
0.07090052217245102,
-0.04756363853812218,
0.023894011974334717,
... |
PMID:20868 | [On the toxicology of carbromal. II. Pharmacokinetics of carbromal and its hypnotically active metabolites in the rat (author's transl)]. | Oral doses up to 20 mg/kg of carbromal and of bromoethylbutyramide were rapidly absorbed in the rat. Absorption from the stomach ligated at the pyloric end was 5-8 fold less than absorption of carbromal injected directly into the small intestine. Oral doses greater than 20 mg/kg of carbromal disappeared more slowly fro... | [On the toxicology of carbromal. II. Pharmacokinetics of carbromal and its hypnotically active metabolites in the rat (author's transl)]. Oral doses up to 20 mg/kg of carbromal and of bromoethylbutyramide were rapidly absorbed in the rat. Absorption from the stomach ligated at the pyloric end was 5-8 fold less than abs... | [
-0.006805779878050089,
-0.026930486783385277,
0.02723216637969017,
0.02208033762872219,
0.01554916426539421,
0.025915957987308502,
0.03385654836893082,
0.012631695717573166,
-0.08042296022176743,
-0.029463982209563255,
0.04392128810286522,
-0.06528971344232559,
-0.0012442857259884477,
0.03... |
PMID:20869 | Separation and quantitative determination of acecarbromal, carbromal, and bromisoval as well as their main metabolites by means of high-pressure liquid chromatographic analysis. | A method is described to separate and quantitatively determine bromoureides and their bromo- and non-bromo-metabolites by means of high-pressure liquid chromatography. This method allows simultaneous measurement of these substances after intake of therapeutic as well as toxic doses. | Separation and quantitative determination of acecarbromal, carbromal, and bromisoval as well as their main metabolites by means of high-pressure liquid chromatographic analysis. A method is described to separate and quantitatively determine bromoureides and their bromo- and non-bromo-metabolites by means of high-pressu... | [
-0.036068715155124664,
-0.023836513981223106,
0.02705683559179306,
0.013130361214280128,
0.03533538058400154,
-0.03070487640798092,
0.04997599497437477,
0.03467586636543274,
-0.05711612477898598,
-0.02493947371840477,
0.00015717819042038172,
-0.07757658511400223,
0.007916202768683434,
-0.0... |
PMID:20870 | Methyl alcohol poisoning. II. Development of a model for ocular toxicity in methyl alcohol poisoning using the rhesus monkey. | Rhesus monkeys were intoxicated with methyl alcohol, using an initial dose of 2 gm/kg and subsequent doses were administered in order to maintain an attenuated and prolonged state of intoxication. Arterial blood samples were drawn for methyl alcohol, formate, PO2, PCO2, and pH, which were monitored periodically through... | Methyl alcohol poisoning. II. Development of a model for ocular toxicity in methyl alcohol poisoning using the rhesus monkey. Rhesus monkeys were intoxicated with methyl alcohol, using an initial dose of 2 gm/kg and subsequent doses were administered in order to maintain an attenuated and prolonged state of intoxicatio... | [
-0.026843983680009842,
0.03464316576719284,
0.01722685806453228,
-0.008426251821219921,
0.04326080530881882,
-0.021171066910028458,
0.06810875236988068,
0.059401243925094604,
-0.0299611184746027,
-0.02367486245930195,
0.015232330188155174,
-0.07832994312047958,
-0.038605257868766785,
0.021... |
PMID:20874 | Serotypes of pneumococci in pneumonia, meningitis and other pneumococcal infections. | During a five-year period, 1965 to 1969 inclusive, pneumococci from 294 patients with acute pneumococcal infections were serotyped. Pneumococci of 33 serotypes were encountered, of which types 3 and 19 were most frequent. The spectrum of infections included pneumonia, meningitis, peritonitis, otitis media and mastoidit... | Serotypes of pneumococci in pneumonia, meningitis and other pneumococcal infections. During a five-year period, 1965 to 1969 inclusive, pneumococci from 294 patients with acute pneumococcal infections were serotyped. Pneumococci of 33 serotypes were encountered, of which types 3 and 19 were most frequent. The spectrum ... | [
-0.0026193715166300535,
0.003614984452724457,
-0.10525789856910706,
-0.017962846904993057,
0.0330701507627964,
0.055814098566770554,
-0.011089978739619255,
0.03825896978378296,
-0.03708336502313614,
0.024600017815828323,
0.007034324109554291,
-0.09279657155275345,
-0.00011316978634567931,
... |
PMID:20876 | Potassium ion-activated hydrolysis of p-nitrophenyl phosphate in pancreatic islet-cell membranes. | Hydrolysis of p-nitrophenyl phosphate was measured in a fraction enriched in plasma membranes from pancreatic islets of non-inbred ob/ob mice. Hydrolysis was stimulated by K+ (10mM) in the pH range 5--10; a small peak of K+-induced activation was observed between pH7.5 and 8. Both the K+-induced activation and the hydr... | Potassium ion-activated hydrolysis of p-nitrophenyl phosphate in pancreatic islet-cell membranes. Hydrolysis of p-nitrophenyl phosphate was measured in a fraction enriched in plasma membranes from pancreatic islets of non-inbred ob/ob mice. Hydrolysis was stimulated by K+ (10mM) in the pH range 5--10; a small peak of K... | [
-0.06720355153083801,
-0.020537393167614937,
-0.004625881556421518,
0.00558667816221714,
-0.012457003816962242,
-0.03950155898928642,
0.04959380254149437,
0.047101181000471115,
-0.0512174591422081,
0.0036379850935190916,
-0.004436412360519171,
-0.07788360118865967,
-0.020957980304956436,
-... |
PMID:20899 | [Determination of the KM-value of the fumarase (E.C. 4.2.1.2) with bencyclan-hydrogenfumarate as the substrate (author's transl)]. | The Michaelis-Menten constant for fumarase (E.C. 4.2.1.2) has been determined by measuring the enzyme activity by the spectrophotometric method of Racker, which depends on the formation or disappearance of the double bond of fumaric acid. When using Na2-fumarate or bencyclan hydrogenfumarate (Fludilat), respectively, a... | [Determination of the KM-value of the fumarase (E.C. 4.2.1.2) with bencyclan-hydrogenfumarate as the substrate (author's transl)]. The Michaelis-Menten constant for fumarase (E.C. 4.2.1.2) has been determined by measuring the enzyme activity by the spectrophotometric method of Racker, which depends on the formation or ... | [
-0.04470283165574074,
0.028957175090909004,
0.018646573647856712,
0.021559977903962135,
0.06769703328609467,
0.006842359434813261,
0.012255694717168808,
0.10056222975254059,
-0.06610529869794846,
-0.05457215756177902,
0.031424008309841156,
-0.10008355975151062,
-0.0487072579562664,
0.01393... |
PMID:20900 | Effects of clozapine and other dibenzo-epines on central dopaminergic and cholinergic systems. Structure-activity relationships. | Structure-activity relationships of 16 dibenzoepines, including clozapine, loxapine, clothiapine and perlapine, have been investigated with regard to locomotor inhibition, cataleptogenesis, apomorphine antagonism, arousal inhibition, effect on striatal dopamine metabolism, and in vivo and in vitro anticholinergic poten... | Effects of clozapine and other dibenzo-epines on central dopaminergic and cholinergic systems. Structure-activity relationships. Structure-activity relationships of 16 dibenzoepines, including clozapine, loxapine, clothiapine and perlapine, have been investigated with regard to locomotor inhibition, cataleptogenesis, a... | [
-0.04515043646097183,
-0.003565598977729678,
0.03661419078707695,
0.053348906338214874,
-0.018746392801404,
0.020121460780501366,
0.026126055046916008,
0.056218672543764114,
0.0025806112680584192,
-0.04651511088013649,
-0.015245388261973858,
-0.02910439483821392,
-0.004421429708600044,
0.0... |
PMID:20903 | Effect of cyclophosphamide on syngeneic transplantation of adenovirus 12-transformed tumor cells in C3H/he mice. | We tested the cyclophosphamide effects against the growth of adenovirus-transformed cells and the subsequent tumor development in the syngeneic host. Cyclophosphamide did not show any effect on the tumor evolution when injected 24 and 6 hours before cell implantation. Cyclophosphamide injected 24 or 39 hours after cell... | Effect of cyclophosphamide on syngeneic transplantation of adenovirus 12-transformed tumor cells in C3H/he mice. We tested the cyclophosphamide effects against the growth of adenovirus-transformed cells and the subsequent tumor development in the syngeneic host. Cyclophosphamide did not show any effect on the tumor evo... | [
-0.05314606800675392,
-0.012676147744059563,
0.0157594196498394,
-0.0054013426415622234,
0.012416149489581585,
-0.004182947333902121,
0.01676584780216217,
0.01066326629370451,
-0.03116765432059765,
-0.021922731772065163,
0.10959448665380478,
-0.020576581358909607,
0.003902225522324443,
-0.... |
PMID:20902 | [Diabetic ketoacidosis in children and adolescents. Clinical and therapeutical considerations in 25 severe cases]. | Twenty-five serious cases of diabetic ketoacidosis, representing 23 patients, with ages ranging from 4 to 15 years are reported. School agers and adolescents were the groups most affected without existing significant predilection for sex. In 40% there was no success in finding the precipitating cause of the crisis; 32%... | [Diabetic ketoacidosis in children and adolescents. Clinical and therapeutical considerations in 25 severe cases]. Twenty-five serious cases of diabetic ketoacidosis, representing 23 patients, with ages ranging from 4 to 15 years are reported. School agers and adolescents were the groups most affected without existing ... | [
-0.022245941683650017,
0.04657329246401787,
0.014444001019001007,
0.020002366974949837,
-0.014073308557271957,
0.0014322391944006085,
0.03474091365933418,
0.044993799179792404,
-0.02539486438035965,
-0.037428341805934906,
0.03270407021045685,
-0.07599122077226639,
-0.013671297580003738,
0.... |
PMID:20911 | Alpha-adrenergic blocking properties of droperidol on isolated blood vessels of the dog. | Concentrations of droperidol which caused a shift to the right of the dose-response curve to noradrenaline in the pulmonary artery and the saphenous vein of the dog did not affect myogenic activation by K+; they did not inhibit spontaneous activity of portal-mesenteric veins. Droperidol inhibited the contractile respon... | Alpha-adrenergic blocking properties of droperidol on isolated blood vessels of the dog. Concentrations of droperidol which caused a shift to the right of the dose-response curve to noradrenaline in the pulmonary artery and the saphenous vein of the dog did not affect myogenic activation by K+; they did not inhibit spo... | [
-0.06261368840932846,
-0.026129815727472305,
0.027078188955783844,
0.011909128166735172,
0.004920704755932093,
-0.06770459562540054,
0.0640174075961113,
0.04733649268746376,
-0.019130809232592583,
-0.047453708946704865,
0.047075606882572174,
-0.006465940736234188,
-0.044799137860536575,
0.... |
PMID:20912 | Respiratory effects of etomidate. | The respiratory effects of etomidate 0.3 mg/kg were studied in patients premedicated with either diazepam and atropine or papaveretum and hyoscine. The incidence of apnoea was 40% in those who received the non-narcotic premedication, compared with 27% in those who received the opiate. Those premedicated with diazepam a... | Respiratory effects of etomidate. The respiratory effects of etomidate 0.3 mg/kg were studied in patients premedicated with either diazepam and atropine or papaveretum and hyoscine. The incidence of apnoea was 40% in those who received the non-narcotic premedication, compared with 27% in those who received the opiate. ... | [
-0.06576455384492874,
-0.009426486678421497,
0.06964100152254105,
0.056869249790906906,
0.039236150681972504,
-0.020980076864361763,
0.023135758936405182,
-0.0068185958079993725,
-0.034719984978437424,
0.016196345910429955,
-0.03546491637825966,
0.07599818706512451,
0.0005554304225370288,
... |
PMID:20913 | Porcine malignant hyperthermia. V: Fatal hyperthermia in the Pietrain pig, associated with the infusion of alpha-adrenergic agonists. | The effects of the administration of noradrenaline alone, and noradrenaline with either phentolamine or propranolol, on thermogenesis and substrate mobilization were investigated in six Pietrain (MH-susceptible) and six Large White (unsusceptible) pigs. The infusion of noradrenaline alone produced a significantly incre... | Porcine malignant hyperthermia. V: Fatal hyperthermia in the Pietrain pig, associated with the infusion of alpha-adrenergic agonists. The effects of the administration of noradrenaline alone, and noradrenaline with either phentolamine or propranolol, on thermogenesis and substrate mobilization were investigated in six ... | [
-0.011126318015158176,
0.010305830277502537,
-0.0026815684977918863,
0.08648236840963364,
0.014928977936506271,
-0.024680882692337036,
0.03878128156065941,
-0.010456521064043045,
-0.022549595683813095,
-0.07121504843235016,
0.008904065936803818,
-0.05180414021015167,
0.022398537024855614,
... |
PMID:20914 | Urinary excretion and metabolism of pethidine and norpethidine in the newborn. | In seven neonates, whose mothers were given pethidine during labour, urine was collected for the first 24-40 h of life. Urinary volume and pH, and concentrations of pethidine and norpethidine in the urine were measured. Urine flow rate was low for the first 7-22 h, and then high for about 12 h. The rate of excretion of... | Urinary excretion and metabolism of pethidine and norpethidine in the newborn. In seven neonates, whose mothers were given pethidine during labour, urine was collected for the first 24-40 h of life. Urinary volume and pH, and concentrations of pethidine and norpethidine in the urine were measured. Urine flow rate was l... | [
-0.023680707439780235,
0.006439811084419489,
0.011620566248893738,
0.00013877185119781643,
0.011262781918048859,
0.025429105386137962,
0.04145592078566551,
-0.02074858546257019,
-0.03793300315737724,
0.008683794178068638,
-0.009291796013712883,
-0.0540761835873127,
0.016855943948030472,
0.... |
PMID:20915 | A spectrofluorometric method for the determination of ajmaline in plasma. | 1 Ajmaline was found to have maximum fluorescence at neutral pH with 300 nm excitation and 365 nm emission wavelengths (corrected). 2 The fluorescence intensity had a linear relationship to concentration up to 50 microgram ml-1 and the recovery of ajmaline after extraction from plasma was 92.5 +/- 3%. 3 Extraction of d... | A spectrofluorometric method for the determination of ajmaline in plasma. 1 Ajmaline was found to have maximum fluorescence at neutral pH with 300 nm excitation and 365 nm emission wavelengths (corrected). 2 The fluorescence intensity had a linear relationship to concentration up to 50 microgram ml-1 and the recovery o... | [
-0.060139309614896774,
0.024178877472877502,
0.029831059277057648,
0.004406820982694626,
-0.005320902448147535,
0.04091225564479828,
0.08192034065723419,
0.032248497009277344,
-0.014476788230240345,
-0.051426537334918976,
0.0038271090015769005,
-0.0603228323161602,
0.019214393571019173,
0.... |
PMID:20916 | A sensitive radioenzymatic assay for adrenaline and noradrenaline in plasma. | 1 An existing radioenzymatic assay for plasma catecholamines using catechol-o-methyl transferase and [3H]-S-adenosyl-methionine has been modified resulting in a more sensitive assay for the measurement of plasma adrenaline and noradrenaline. 2 The lower limit of sensitivity for this method is 25 pg for adrenaline and 3... | A sensitive radioenzymatic assay for adrenaline and noradrenaline in plasma. 1 An existing radioenzymatic assay for plasma catecholamines using catechol-o-methyl transferase and [3H]-S-adenosyl-methionine has been modified resulting in a more sensitive assay for the measurement of plasma adrenaline and noradrenaline. 2... | [
-0.04163892939686775,
0.02601797692477703,
-0.027932869270443916,
0.05242817476391792,
0.014552656561136246,
-0.01903020776808262,
0.0714142844080925,
0.014272796921432018,
-0.017632680013775826,
-0.05992814898490906,
0.014328845776617527,
-0.05568574741482735,
-0.00496289785951376,
0.0832... |
PMID:20917 | Effect of the 1,5-benzodiazepines, clobazam and triflubazam, on sleep in man. | 1 The effect of the 1,5-benzodiazepines, clobazam (10 and 20 mg) and triflubazam (20 and 40 mg), on sleep was studied in six healthy males using electroencephalography for sleep measures and analogue scales for subjective assessments of well being and sleep quality. The effect of clobazam was limited to the night of in... | Effect of the 1,5-benzodiazepines, clobazam and triflubazam, on sleep in man. 1 The effect of the 1,5-benzodiazepines, clobazam (10 and 20 mg) and triflubazam (20 and 40 mg), on sleep was studied in six healthy males using electroencephalography for sleep measures and analogue scales for subjective assessments of well ... | [
-0.021129392087459564,
0.04072631895542145,
0.028521668165922165,
0.08143114298582077,
0.004529955331236124,
0.012896688655018806,
0.06110506132245064,
0.02378104440867901,
-0.028091974556446075,
-0.03581589087843895,
-0.020168723538517952,
-0.009325332939624786,
0.013905179686844349,
0.00... |
PMID:20918 | Clobazam, a 1,5-benzodiazepine, and car-driving ability. | 1 The effects of clobazam, a new anxiolytic agent (a 1,5-benzodiazepine) on car-driving ability and other tests of psychomotor performance were investigated in a double-blind, cross-over study v. placebo in normal volunteers. 2 Clobazam (20 mg) or placebo was given nightly for six nights to ten volunteers and subjectiv... | Clobazam, a 1,5-benzodiazepine, and car-driving ability. 1 The effects of clobazam, a new anxiolytic agent (a 1,5-benzodiazepine) on car-driving ability and other tests of psychomotor performance were investigated in a double-blind, cross-over study v. placebo in normal volunteers. 2 Clobazam (20 mg) or placebo was giv... | [
-0.02870141714811325,
0.01168710459023714,
0.036496035754680634,
0.05116092413663864,
-0.015506505034863949,
0.0692799985408783,
0.07899749279022217,
0.02224101684987545,
-0.044720038771629333,
-0.03421589732170105,
-0.014143799431622028,
0.007830165326595306,
-0.0035398234613239765,
0.013... |
PMID:20919 | Further studies of rimiterol and salbutamol administered by intermittent positive-pressure ventilation, and an important observation on the technique of using the Bennett ventilator. | 1. Using the same technique of administering drugs by intermittent positive-pressure ventilation as used in previous studies a source of contamination of solutions nebulized was discovered. This was rectified by using a new ventilator and completely separate patient circuits for each solution nebulized. 2 Salbutamol 0.... | Further studies of rimiterol and salbutamol administered by intermittent positive-pressure ventilation, and an important observation on the technique of using the Bennett ventilator. 1. Using the same technique of administering drugs by intermittent positive-pressure ventilation as used in previous studies a source of ... | [
0.004642861895263195,
-0.0015945576597005129,
0.01195905264467001,
-0.023746799677610397,
0.007703221403062344,
0.029512302950024605,
0.054867882281541824,
0.02777138538658619,
-0.047167520970106125,
-0.08854128420352936,
-0.0059953853487968445,
-0.04952792823314667,
-0.010194317437708378,
... |
PMID:20922 | The excretion of gamma-glutamyl transferase in pregnancy. | A cross-sectional study of gamma-glutamyl transferase (gamma-GT) excretion in 316 healthy pregnant women showed the enzyme excretion (mU per mmol creatinine) was significantly higher than that of a non-pregnant control group. The increase was apparent before the end of the first trimester and continued therafter. These... | The excretion of gamma-glutamyl transferase in pregnancy. A cross-sectional study of gamma-glutamyl transferase (gamma-GT) excretion in 316 healthy pregnant women showed the enzyme excretion (mU per mmol creatinine) was significantly higher than that of a non-pregnant control group. The increase was apparent before the... | [
0.01914742775261402,
-0.022254955023527145,
-0.020281294360756874,
0.05588570237159729,
0.01473153941333294,
0.005295668262988329,
0.06738096475601196,
0.08419573307037354,
-0.04540509358048439,
-0.027263835072517395,
0.001759590464644134,
-0.0275583453476429,
-0.01883942075073719,
0.04636... |
PMID:20927 | Calcium-induced cooperativity of manganese binding to concanavalin A. | Titrations employing electron spin resonance spectroscopy and equilibrium dialysis studies have revealed that Mn2+ binding to concanavalin A is cooperative in the presence and noncooperative in the absence of Ca2+. The degree of cooperativity increases with increasing pH. Hill coefficients range from 1.4 at pH 5.0 to 1... | Calcium-induced cooperativity of manganese binding to concanavalin A. Titrations employing electron spin resonance spectroscopy and equilibrium dialysis studies have revealed that Mn2+ binding to concanavalin A is cooperative in the presence and noncooperative in the absence of Ca2+. The degree of cooperativity increas... | [
-0.0976875051856041,
-0.007574295159429312,
-0.06539596617221832,
0.010623692534863949,
0.00521621061488986,
-0.061539627611637115,
0.08680147677659988,
0.037124380469322205,
-0.02849224954843521,
-0.029937734827399254,
0.047744832932949066,
-0.11635193228721619,
0.017764689400792122,
0.02... |
PMID:20929 | Spontaneous, reversible protein cross-linking in the human erythrocyte membrane. Temperature and pH dependence. | Changes in pH significantly affect the morphology and physical properties of red cell membranes. We have explored the molecular basis for these phenomena by characterizing the pattern of protein disulfide cross-linkages formed spontaneously in ghost exposed to acid pH or elevated temperature (37 degrees C). Protein agg... | Spontaneous, reversible protein cross-linking in the human erythrocyte membrane. Temperature and pH dependence. Changes in pH significantly affect the morphology and physical properties of red cell membranes. We have explored the molecular basis for these phenomena by characterizing the pattern of protein disulfide cro... | [
-0.1060696691274643,
-0.04753759130835533,
-0.033568382263183594,
0.04298599809408188,
0.031163189560174942,
-0.011042526923120022,
0.08132781088352203,
0.02393367886543274,
-0.0100595997646451,
-0.01141715794801712,
0.0036450354382395744,
-0.08725368231534958,
0.027498068287968636,
0.0458... |
PMID:20930 | Kinetics of binding of oligosaccharides to a homogeneous pneumococcal antibody: dependence on antigen chain length suggests a labile intermediate complex. | Temperature-jump experiments were performed with di-, tetra-, and hexasaccharides derived from type III pneumococcal polysaccharide using a homogeneous corresponding antibody IgG 45-394. A decrease in stability of the oligosaccharide-antibody complexes with decreasing chain length was observed and entirely reflected in... | Kinetics of binding of oligosaccharides to a homogeneous pneumococcal antibody: dependence on antigen chain length suggests a labile intermediate complex. Temperature-jump experiments were performed with di-, tetra-, and hexasaccharides derived from type III pneumococcal polysaccharide using a homogeneous corresponding... | [
-0.1175164133310318,
0.017267145216464996,
-0.05915023013949394,
0.007142477203160524,
0.025205129757523537,
0.025782771408557892,
0.007172013632953167,
-0.008511366322636604,
-0.02639973722398281,
0.015249449759721756,
0.041055355221033096,
-0.07148747891187668,
0.010704223066568375,
-0.0... |
PMID:20932 | Interaction of zinc and hemoglobin: binding of zinc and the oxygen affinity. | Stripped human hemoglobin was shown to have a high apparent zinc association constant of 1.3 X 10(7) M-1 with a stoichiometry of one zinc for every two hemes. The saturation of this site produces a dramatic 3.7-fold increase in the oxygen affinity. The effect of zinc on the oxygen affinity is interrelated with the inte... | Interaction of zinc and hemoglobin: binding of zinc and the oxygen affinity. Stripped human hemoglobin was shown to have a high apparent zinc association constant of 1.3 X 10(7) M-1 with a stoichiometry of one zinc for every two hemes. The saturation of this site produces a dramatic 3.7-fold increase in the oxygen affi... | [
-0.09764404594898224,
0.11386806517839432,
-0.03994407132267952,
0.041814908385276794,
0.050207097083330154,
-0.03630560636520386,
0.09063776582479477,
0.02535950019955635,
-0.07185087352991104,
0.008243318647146225,
0.0480511412024498,
-0.07377170771360397,
0.033812351524829865,
0.0258426... |
PMID:20934 | Spectrophotometric titration of a single carboxyl group at the active site of ribonuclease T1. | Low-pH-induced difference spectra for ribonuclease T1, which were determined using a reference solution at pH 6, consisted of a shorter wavelength component from 270 to 285 nm that relfected an ionization having a pKa of 3.54 and a longer wavelength component above 285 nm that reflected an ionization having a pKa of 4.... | Spectrophotometric titration of a single carboxyl group at the active site of ribonuclease T1. Low-pH-induced difference spectra for ribonuclease T1, which were determined using a reference solution at pH 6, consisted of a shorter wavelength component from 270 to 285 nm that relfected an ionization having a pKa of 3.54... | [
-0.07879277318716049,
-0.0033097919076681137,
-0.04925534129142761,
0.011632761918008327,
0.029692640528082848,
0.004508765414357185,
0.03334985300898552,
0.049884550273418427,
-0.025454329326748848,
-0.0053469534032046795,
0.057477403432130814,
-0.06056031957268715,
0.02052861452102661,
0... |
PMID:20935 | Selective phosphorylation of erythrocyte membrane proteins by the solubilized membrane protein kinases. | This report describes the substrate and phosphoryl donor specificities of solubilized erythrocyte membrane cyclic adenosine 3',5'-monophosphate (cAMP)-independent protein kinases toward human and rabbit erythrocyte membrane proteins. Three types of substrate preparations have been utilized: heat-inactivated ghosts, iso... | Selective phosphorylation of erythrocyte membrane proteins by the solubilized membrane protein kinases. This report describes the substrate and phosphoryl donor specificities of solubilized erythrocyte membrane cyclic adenosine 3',5'-monophosphate (cAMP)-independent protein kinases toward human and rabbit erythrocyte m... | [
-0.07029728591442108,
-0.05951201170682907,
-0.015265708789229393,
-0.014307460747659206,
0.016150914132595062,
-0.02592426724731922,
0.04057926684617996,
0.04795420914888382,
-0.058538179844617844,
-0.019727686420083046,
0.03607824072241783,
-0.06707310676574707,
-0.03836572915315628,
-0.... |
PMID:20940 | Effect of H+ on the K+ activation of adenosine-5'-monophosphate aminohydrolase. | The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several e... | Effect of H+ on the K+ activation of adenosine-5'-monophosphate aminohydrolase. The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K... | [
-0.10000278800725937,
-0.023415302857756615,
-0.0065976958721876144,
0.05375880375504494,
0.006511174142360687,
-0.031995270401239395,
0.06829119473695755,
0.014420784078538418,
-0.015363622456789017,
-0.026164527982473373,
0.05878852680325508,
-0.07081510871648788,
-0.04328581690788269,
0... |
PMID:20941 | Phosphorylation of nuclear proteins in rat regenerating liver. | In studies of the phosphorylated proteins in rat liver and Walker-256, it was established that the ratio of various fractions of P-N linkages to P-O linkages varies from 0.6 to 3.1. In rat regenerating liver nuclei, the ratio of P-N and P-O varies with time after partial hepatectomy. Using [3H]-lysine and 32Pi, it is s... | Phosphorylation of nuclear proteins in rat regenerating liver. In studies of the phosphorylated proteins in rat liver and Walker-256, it was established that the ratio of various fractions of P-N linkages to P-O linkages varies from 0.6 to 3.1. In rat regenerating liver nuclei, the ratio of P-N and P-O varies with time... | [
-0.06172633543610573,
-0.05101972818374634,
0.023500608280301094,
-0.07587163150310516,
0.013023914769291878,
-0.04660727083683014,
0.04249709099531174,
-0.015847831964492798,
-0.07038124650716782,
0.020412199199199677,
0.05059381574392319,
-0.06569920480251312,
-0.03441757336258888,
0.049... |
PMID:20943 | Effect of pressure upon the fluorescence of various flavodoxins. | The effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from Peptostreptococcus elsdenii, Desulfovibrio vulgaris, Azotobacter vinelandii, and Clostridium MP were investigated. The first three flavoproteins showed under high pressure enhancements of flavin fluorescence of... | Effect of pressure upon the fluorescence of various flavodoxins. The effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from Peptostreptococcus elsdenii, Desulfovibrio vulgaris, Azotobacter vinelandii, and Clostridium MP were investigated. The first three flavoproteins ... | [
-0.06429808586835861,
-0.03231851011514664,
0.025360198691487312,
0.05344844609498978,
0.035626161843538284,
-0.0057824840769171715,
0.04726975038647652,
0.03310564160346985,
-0.02613520249724388,
-0.011688572354614735,
-0.04622170701622963,
-0.03985386714339256,
0.016299854964017868,
-0.0... |
PMID:20944 | Occurrence of fatty acid chlorohydrins in jellyfish lipids. | Fatty acid chlorohydrins are characterized as lipid components of an edible jellyfish. The four isomers 9-chloro-10-hydroxypalmitic acid, 10-chloro-9-hydroxypalmitic acid, 9-chloro-10-hydroxystearic acid, and 10-chloro-9-hydroxystearic acid were identified by gas chromatography-mass spectrometry comparison of the methy... | Occurrence of fatty acid chlorohydrins in jellyfish lipids. Fatty acid chlorohydrins are characterized as lipid components of an edible jellyfish. The four isomers 9-chloro-10-hydroxypalmitic acid, 10-chloro-9-hydroxypalmitic acid, 9-chloro-10-hydroxystearic acid, and 10-chloro-9-hydroxystearic acid were identified by ... | [
-0.038622502237558365,
-0.014712401665747166,
-0.02481730841100216,
-0.01783107779920101,
0.01723124459385872,
0.028346218168735504,
0.0070637590251863,
0.05098485201597214,
-0.06261143833398819,
-0.029211733490228653,
0.04000861942768097,
-0.04240233451128006,
0.006796741392463446,
-0.069... |
PMID:20946 | Steady-state kinetics of electron transfer through cytochrome chain of uncoupled submitochondrial particles. I. General kinetic analysis. | Steady-state kinetics of electron transfer through the cytochrome chain of uncoupled ultrasonic submitochondrial particles at different pH values has been studied. Rate constants calculated from the Pring equation (ki' = V/PirPi+1ox) increased with the increase of the rate of the process. As in the previous work (Saks,... | Steady-state kinetics of electron transfer through cytochrome chain of uncoupled submitochondrial particles. I. General kinetic analysis. Steady-state kinetics of electron transfer through the cytochrome chain of uncoupled ultrasonic submitochondrial particles at different pH values has been studied. Rate constants cal... | [
-0.08902202546596527,
0.018799234181642532,
-0.04562142863869667,
0.04491792619228363,
0.0030777230858802795,
-0.022537052631378174,
0.02200973778963089,
0.007116328459233046,
0.019059423357248306,
0.019977964460849762,
0.051618821918964386,
-0.05418357253074646,
0.0011691804975271225,
0.0... |
PMID:20947 | Conformation-dependent participation of the protein in electron equivalent transfer to cytochrome c. | When ferricytochrome c is reduced by H atoms (produced by pulse radiolysis) at neutral pH where it is in a closed protein configuration, a considerable percentage of the reduction proceeds through electron equivalent transfer via the protein. At pH 2.0, where cytochrome c is in an open configuration, H atoms reduce by ... | Conformation-dependent participation of the protein in electron equivalent transfer to cytochrome c. When ferricytochrome c is reduced by H atoms (produced by pulse radiolysis) at neutral pH where it is in a closed protein configuration, a considerable percentage of the reduction proceeds through electron equivalent tr... | [
-0.06089039519429207,
0.0598360113799572,
-0.08306621015071869,
0.02411569468677044,
-0.0030278631020337343,
-0.03518078103661537,
0.08555571734905243,
0.015755414962768555,
-0.030247215181589127,
0.018122419714927673,
0.03422379493713379,
-0.07487428933382034,
-0.01700476184487343,
0.0567... |
PMID:20948 | Site of bicarbonate effect in Hill reaction. Evidence from the use of artificial electron acceptors and donors. | Using artificial electron donors and acceptors, it is shown here that the major HCO3- effect in the Hill reaction is after the "primary" electron acceptor (Q) of Photosystem II and before the site of action of 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (at the plastoquinone pool). Chloroplasts in the presence of b... | Site of bicarbonate effect in Hill reaction. Evidence from the use of artificial electron acceptors and donors. Using artificial electron donors and acceptors, it is shown here that the major HCO3- effect in the Hill reaction is after the "primary" electron acceptor (Q) of Photosystem II and before the site of action o... | [
-0.09706485271453857,
-0.0197325199842453,
-0.02678830921649933,
0.07034318149089813,
-0.005728765856474638,
-0.00794124510139227,
0.0030096489936113358,
-0.03257682919502258,
-0.046793464571237564,
-0.016845067963004112,
0.06147785857319832,
-0.055839311331510544,
-0.047823186963796616,
0... |
PMID:20950 | Interactions of Tetrahymena dynein with microtubule protein. Tubulin-induced stimulation of dynein ATPase activity. | The ATPase (EC 3.6.1.3) activity of 30 S dynein from Tetrahymena cilia was remarkably stimulated by porcine brain tubulin at pH 10. The activity increased with increasing concentration of tubulin until the molar ratio of tubulin dimer to 30 S dynein reached approx. 10. The optimum of the ATPase activity of 30 S dynein ... | Interactions of Tetrahymena dynein with microtubule protein. Tubulin-induced stimulation of dynein ATPase activity. The ATPase (EC 3.6.1.3) activity of 30 S dynein from Tetrahymena cilia was remarkably stimulated by porcine brain tubulin at pH 10. The activity increased with increasing concentration of tubulin until th... | [
-0.0725521519780159,
-0.056651771068573,
0.014072480611503124,
0.026726674288511276,
-0.0007744853501208127,
-0.04069418087601662,
0.02711748704314232,
0.02757781371474266,
0.017702627927064896,
-0.05500507727265358,
-0.005938160698860884,
-0.07342740148305893,
-0.03272970765829086,
0.0294... |
PMID:20951 | Non-covalent cross-linking of lipid bilayers by myelin basic protein: a possible role in myelin formation. | Myelin basic protein associates with bilayer vesicles of pure egg phosphatidylcholine, L-alpha-dimyristoyl phosphatidylcholine and DL-alpha-dipalmitoyl phosphatidylcholine. Under optimum conditions the vesicles contain 15-18% of protein by weight. The binding to dipalmitoyl phosphatidylcholine is facilitated above its ... | Non-covalent cross-linking of lipid bilayers by myelin basic protein: a possible role in myelin formation. Myelin basic protein associates with bilayer vesicles of pure egg phosphatidylcholine, L-alpha-dimyristoyl phosphatidylcholine and DL-alpha-dipalmitoyl phosphatidylcholine. Under optimum conditions the vesicles co... | [
-0.08291910588741302,
-0.049036409705877304,
-0.02813330665230751,
-0.03327838331460953,
-0.0698055848479271,
-0.036899860948324203,
0.07430347055196762,
0.035462260246276855,
-0.03573811054229736,
-0.049419861286878586,
0.019116763025522232,
-0.11026985198259354,
0.05493524298071861,
0.03... |
PMID:20952 | Contrast manifestation of alkaline phosphatase and 5'-nucleotidase in plasma membranes isolated from rat liver and ascites hepatoma. | 1. Plasma membranes were isolated from ascites hepatoma AH-130 and rat livers with or without partial hepatectomy or bile duct ligation. Reciprocal manifestations of two marker enzymes for plasma membranes were observed in these membrane preparations; alkaline phosphatase activity was found much higher in the hepatoma ... | Contrast manifestation of alkaline phosphatase and 5'-nucleotidase in plasma membranes isolated from rat liver and ascites hepatoma. 1. Plasma membranes were isolated from ascites hepatoma AH-130 and rat livers with or without partial hepatectomy or bile duct ligation. Reciprocal manifestations of two marker enzymes fo... | [
-0.02619471587240696,
-0.03395473212003708,
-0.005363721866160631,
0.015369376167654991,
-0.005935376975685358,
-0.06158900633454323,
0.09750424325466156,
0.0846526026725769,
-0.0382457934319973,
-0.04544229060411453,
0.03743116930127144,
-0.03702060133218765,
-0.03872131556272507,
-0.0017... |
PMID:20953 | Partition of catalase and its peroxidase activities in human red cell membrane: effect of ATP depletion. | Partititon of catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase EC 1.11.1.6) and peroxidase (donor:hydrogen-peroxide oxidoreductase EC 1.11.1.7) activities between the red cell membrane and the cytosol were studied under various experimental conditions. A small but significant amount of catalase (1.6%) was r... | Partition of catalase and its peroxidase activities in human red cell membrane: effect of ATP depletion. Partititon of catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase EC 1.11.1.6) and peroxidase (donor:hydrogen-peroxide oxidoreductase EC 1.11.1.7) activities between the red cell membrane and the cytosol we... | [
-0.0831383466720581,
-0.02266469970345497,
-0.022617865353822708,
0.028628800064325333,
0.022136565297842026,
-0.03720369189977646,
0.05729677900671959,
0.0621584989130497,
-0.0035483965184539557,
-0.01080732885748148,
-0.004170273430645466,
-0.053377021104097366,
-0.0032045200932770967,
0... |
PMID:20955 | Polyribonucleotides containing thiopurines. Synthesis and properties of poly(1-methyl-6-thioguanylic acid). | The synthesis of 1-methyl-6-thioguanosine 5'-diphosphate and its conversion to poly(1-methyl-6-thioguanylic acid) by means of polynucleotide phosphorylase are described. The polymer exhibited cooperative behavior (Tm = 294 K in the absence of added NaCl) characteristic of a highly stacked single-stranded helical array.... | Polyribonucleotides containing thiopurines. Synthesis and properties of poly(1-methyl-6-thioguanylic acid). The synthesis of 1-methyl-6-thioguanosine 5'-diphosphate and its conversion to poly(1-methyl-6-thioguanylic acid) by means of polynucleotide phosphorylase are described. The polymer exhibited cooperative behavior... | [
-0.06922394782304764,
-0.05671699717640877,
-0.002706824103370309,
-0.005736191291362047,
-0.040952760726213455,
0.014604191295802593,
0.010656904429197311,
0.03222993016242981,
-0.03619297221302986,
-0.0000805248855613172,
0.033832453191280365,
-0.03126325085759163,
0.007087993435561657,
... |
PMID:20956 | Biphasic effects of translational inhibitors on liver tyrosine aminotransferase. | Low doses of cycloheximide or emetine cause rat liver tyrosine aminotransferase activity to rise up to twice the control levels in 2 h. By contrast, in the same interval no changes, or only a slight decrease, are produced by either drug at high dosage. Adrenalectomised animals display the same pattern of response. High... | Biphasic effects of translational inhibitors on liver tyrosine aminotransferase. Low doses of cycloheximide or emetine cause rat liver tyrosine aminotransferase activity to rise up to twice the control levels in 2 h. By contrast, in the same interval no changes, or only a slight decrease, are produced by either drug at... | [
-0.05054773762822151,
0.03395433723926544,
-0.004218052141368389,
0.038205694407224655,
-0.05709400400519371,
-0.047572243958711624,
0.07280374318361282,
0.0011436023050919175,
-0.025141222402453423,
-0.028368648141622543,
0.022136354818940163,
-0.02703658677637577,
0.06613486260175705,
0.... |
PMID:20957 | Fluorimetric assay of tobacco leaf dehydrogenases with resazurin. | A versatile fluorimetric assay based on the reduction of resazurin to resorufin demonstrated high specific activities for a number of important pyridine nucleotide-linked dehydrogenases in tobacco leaves. The Michaelis constant for the important photosynthetic enzyme, D-glyceraldehyde-3-phosphate:NADP+ oxidoreductase (... | Fluorimetric assay of tobacco leaf dehydrogenases with resazurin. A versatile fluorimetric assay based on the reduction of resazurin to resorufin demonstrated high specific activities for a number of important pyridine nucleotide-linked dehydrogenases in tobacco leaves. The Michaelis constant for the important photosyn... | [
-0.04341090843081474,
0.026999179273843765,
-0.04836491122841835,
0.04513445124030113,
0.08728896826505661,
0.0010087410919368267,
0.011863642372190952,
0.029691090807318687,
-0.06186695396900177,
-0.0412256196141243,
0.003552520414814353,
-0.06742646545171738,
0.017568493261933327,
-0.009... |
PMID:20958 | Purification and characterization of 2-oxoaldehyde dehydrogenase from rat liver. | The partial purification (123-fold) of 2-oxoaldehyde dehydrogenase (2-oxoaldehyde:NAD(P)+ oxidoreductase, 1.2.1.23) from rat liver was carried out using a purification procedure which involved (NH4)2SO4 fractionation, DEAE-Sephadex chromatography, Blue-Dextran affinity chromatography and CM-Sephadex chromatography. A s... | Purification and characterization of 2-oxoaldehyde dehydrogenase from rat liver. The partial purification (123-fold) of 2-oxoaldehyde dehydrogenase (2-oxoaldehyde:NAD(P)+ oxidoreductase, 1.2.1.23) from rat liver was carried out using a purification procedure which involved (NH4)2SO4 fractionation, DEAE-Sephadex chromat... | [
-0.06474500894546509,
0.02709697186946869,
-0.07164621353149414,
-0.016005419194698334,
0.052841752767562866,
-0.04597974941134453,
0.010969611816108227,
-0.006450900807976723,
-0.056071437895298004,
-0.04203937202692032,
0.03708410635590553,
-0.08038629591464996,
0.05791790410876274,
-0.0... |
PMID:20959 | Oxidation of hypoxanthines, bearing 8-aryl or 8-pyridyl substituents, by bovine milk xanthine oxidase. | 1. Hypoxanthines, bearing at position 8 aryl or pyridyl substituents, are converted by bovine milk xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.2.3.2) into the corresponding xanthines at low rates. Oxidation is accelerated considerably when the 8-pyridyl substituents are quaternised. 2. In the enzymic oxidat... | Oxidation of hypoxanthines, bearing 8-aryl or 8-pyridyl substituents, by bovine milk xanthine oxidase. 1. Hypoxanthines, bearing at position 8 aryl or pyridyl substituents, are converted by bovine milk xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.2.3.2) into the corresponding xanthines at low rates. Oxidatio... | [
-0.07334034144878387,
-0.03807615488767624,
-0.053425177931785583,
0.06003591790795326,
0.05199771746993065,
-0.003717792686074972,
-0.030528705567121506,
0.008655741810798645,
-0.03225599601864815,
0.034508783370256424,
-0.021334141492843628,
-0.044297728687524796,
0.018915614113211632,
-... |
PMID:20960 | Evidence about the catecholoxidase activity of the enzyme ascorbate oxidase extracted from Cucurbita pepo medullosa. | Pure ascorbate oxidase (L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) isolated from Cucurbita pepo medullosa, which is known to be specific for ascorbic acid, shows a secondary catecholoxidase activity at approx. pH 6.7. This activity was tested against natural and synthetic compounds possessing a catechol-like struc... | Evidence about the catecholoxidase activity of the enzyme ascorbate oxidase extracted from Cucurbita pepo medullosa. Pure ascorbate oxidase (L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) isolated from Cucurbita pepo medullosa, which is known to be specific for ascorbic acid, shows a secondary catecholoxidase activity... | [
-0.029018141329288483,
0.004042485728859901,
-0.08174913376569748,
0.0347319021821022,
0.05603322759270668,
-0.05089673772454262,
0.05789029970765114,
-0.008945353329181671,
-0.0029858259949833155,
-0.021845733746886253,
-0.019016936421394348,
-0.06739488989114761,
0.023795831948518753,
0.... |
PMID:20962 | Canine thyroid fucokinase. | A radiometric assay was developed for fucokinase (ATP:6-deoxy-L-galactose 1-phosphotransferase, EC 2.7.1.52) based on the conversion of L-[14C]fucose to L-[14C]fucose 1-phosphate which is trapped and counted on ion exchange paper. This assay was used to detect the presence of a fucokinase in canine thyroid tissue which... | Canine thyroid fucokinase. A radiometric assay was developed for fucokinase (ATP:6-deoxy-L-galactose 1-phosphotransferase, EC 2.7.1.52) based on the conversion of L-[14C]fucose to L-[14C]fucose 1-phosphate which is trapped and counted on ion exchange paper. This assay was used to detect the presence of a fucokinase in ... | [
-0.10211744904518127,
-0.0691889077425003,
-0.0023224300239235163,
-0.001287741819396615,
0.022295789793133736,
-0.03547241911292076,
0.050591472536325455,
0.10766728222370148,
-0.08958399295806885,
-0.002882191212847829,
0.06623942404985428,
-0.11209052056074142,
-0.08710481226444244,
0.0... |
PMID:20963 | The mode of binding of potential transition-state analogs to acetylcholinesterase. | Phenylacetone, 4-phenyl-2-butanone, and 4-oxopentyltrimethylammonium chloride were tested as potential transition state analogs for eel acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7). Phenylacetone is a competitive inhibitor of the enzyme but not a transition state analog, since its binding constant is simi... | The mode of binding of potential transition-state analogs to acetylcholinesterase. Phenylacetone, 4-phenyl-2-butanone, and 4-oxopentyltrimethylammonium chloride were tested as potential transition state analogs for eel acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7). Phenylacetone is a competitive inhibitor ... | [
-0.0961076095700264,
-0.009742810390889645,
-0.04133358597755432,
0.04964405298233032,
-0.015741653740406036,
0.0038619113620370626,
0.08283483982086182,
0.02692803554236889,
-0.03263931721448898,
-0.019460417330265045,
0.009815727360546589,
-0.03461860120296478,
0.016436288133263588,
0.02... |
PMID:20964 | pH dependence and solvent isotope effects in the hydrolysis of phosphomonoesters by human prostatic acid phosphatase. | The pH dependence of the human prostatic acid phosphatase-catalyzed hydrolysis of p-nitrophenyl phosphate and beta-glyceryl phosphate has been studied over a wide range of pH and the values of Km and V calculated with the aid of the Cleland HYPER program. The pH dependence of Km shows the effect of substrate ionization... | pH dependence and solvent isotope effects in the hydrolysis of phosphomonoesters by human prostatic acid phosphatase. The pH dependence of the human prostatic acid phosphatase-catalyzed hydrolysis of p-nitrophenyl phosphate and beta-glyceryl phosphate has been studied over a wide range of pH and the values of Km and V ... | [
-0.05574372038245201,
0.03218046575784683,
0.0022698170505464077,
0.012331495061516762,
0.020075181499123573,
-0.04989822208881378,
0.024284280836582184,
0.02592366561293602,
-0.0565805658698082,
-0.025535570457577705,
0.07808426022529602,
-0.028694286942481995,
0.0011512404307723045,
0.04... |
PMID:20966 | Proton magnetic resonance studies of histidines in human, rhesus monkey, and bovine carbonic anhydrases. | Histidine C-2 proton resonances in rhesus monkey carbonic anhydrase B (carbonate hydro-lyase, EC 4.2.1.1) and bovine carbonic anhydrase were investigated using 270-MHz proton magnetic resonance. The results suggest that there are extensive three-dimensional homologies between the human B and rhesus B enzymes and betwee... | Proton magnetic resonance studies of histidines in human, rhesus monkey, and bovine carbonic anhydrases. Histidine C-2 proton resonances in rhesus monkey carbonic anhydrase B (carbonate hydro-lyase, EC 4.2.1.1) and bovine carbonic anhydrase were investigated using 270-MHz proton magnetic resonance. The results suggest ... | [
-0.07293001562356949,
0.03843039274215698,
-0.09588214755058289,
0.0015030283248052,
0.03158021345734596,
-0.039547473192214966,
0.032547879964113235,
0.023378506302833557,
-0.01419228408485651,
-0.04341556876897812,
0.009150772355496883,
-0.06995265185832977,
-0.026142768561840057,
0.0071... |
PMID:20967 | Kinetic analysis of calcium activation of brain acetylcholinesterase forms. | Calcium activation of acetylcholine hydrolysis by bovine brain acetylcholinesterase (Acetylcholine hydrolase, EC 3.1.1.7) forms has been analyzed in terms of changes in kinetic constants and thermodynamic activation parameters. De-acetylation was determined to be the major rate-influencing step in acetylcholine hydroly... | Kinetic analysis of calcium activation of brain acetylcholinesterase forms. Calcium activation of acetylcholine hydrolysis by bovine brain acetylcholinesterase (Acetylcholine hydrolase, EC 3.1.1.7) forms has been analyzed in terms of changes in kinetic constants and thermodynamic activation parameters. De-acetylation w... | [
-0.089863620698452,
-0.003361027454957366,
-0.031186042353510857,
0.0488714724779129,
-0.05134350806474686,
-0.010182840749621391,
0.047537337988615036,
0.04595047980546951,
0.019147025421261787,
-0.032955240458250046,
0.013919220305979252,
-0.06781615316867828,
-0.036626510322093964,
0.01... |
PMID:20969 | Resolution of independently titrating spectral components in the ultraviolet circular dichroism of subtilisin enzymes by matrix rank analysis. | The ultraviolet circular dichroism of di-isopropylphophoryl-subtilisins Carlsberg and Novo (EC 3.4.21.14) has been examined as a function of pH. The CD of these enzymes below 260 nm is invariant over the pH interval 4 to 12, below or above which spectral changes occur suggesting a transition to a random coil form. Abov... | Resolution of independently titrating spectral components in the ultraviolet circular dichroism of subtilisin enzymes by matrix rank analysis. The ultraviolet circular dichroism of di-isopropylphophoryl-subtilisins Carlsberg and Novo (EC 3.4.21.14) has been examined as a function of pH. The CD of these enzymes below 26... | [
-0.10544852912425995,
-0.01788415014743805,
-0.06837470084428787,
0.014813519082963467,
0.02945825457572937,
-0.0067129130475223064,
0.0597086176276207,
0.039644159376621246,
-0.015561984851956367,
0.007486450020223856,
0.0660681277513504,
-0.08772269636392593,
0.03816049546003342,
0.03016... |
PMID:20971 | Some characteristics of soluble fatty acid synthesis in germinating pea seeds. | Soluble fractions from germinating pea synthesize palmitic acid de novo and stearic acid by elongation. Malonyl CoA, acyl carrier protein and NADPH are required for both reactions. In contrast to some other plant systems, no requirement was found for divalent cations. On the other hand, the formation of both stearate a... | Some characteristics of soluble fatty acid synthesis in germinating pea seeds. Soluble fractions from germinating pea synthesize palmitic acid de novo and stearic acid by elongation. Malonyl CoA, acyl carrier protein and NADPH are required for both reactions. In contrast to some other plant systems, no requirement was ... | [
-0.030411576852202415,
0.01555649284273386,
-0.01447979174554348,
0.029172470793128014,
0.011200166307389736,
-0.016268648207187653,
0.010287577286362648,
-0.0023683281615376472,
-0.058081526309251785,
0.011838414706289768,
0.02519949898123741,
-0.13838006556034088,
0.05528523772954941,
-0... |
PMID:20974 | Immunoelectrophoretic studies on pig intestinal brush border proteins. | Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolated and shown by the double layer immunofluorescence staining technique t... | Immunoelectrophoretic studies on pig intestinal brush border proteins. Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolate... | [
-0.051743946969509125,
-0.006426840089261532,
-0.002299971878528595,
0.051873184740543365,
0.027425911277532578,
-0.0876963809132576,
0.06490607559680939,
0.05014466121792793,
-0.04796028509736061,
0.014855995774269104,
-0.008131377398967743,
-0.10309503972530365,
-0.035503532737493515,
0.... |
PMID:20976 | Properties of trout hemoglobin covalently bound to a solid matrix. | This paper reports the ligand binding properties of the major hemoglobin component from trout (Salmo irideus) covalently bound to a solid matrix (Sepharose or Sephadex). A comparison between the functional properties of this protein in solution and of the protein-matrix complex shows significant changes although the ba... | Properties of trout hemoglobin covalently bound to a solid matrix. This paper reports the ligand binding properties of the major hemoglobin component from trout (Salmo irideus) covalently bound to a solid matrix (Sepharose or Sephadex). A comparison between the functional properties of this protein in solution and of t... | [
-0.11052349209785461,
0.027693498879671097,
-0.03200910612940788,
-0.011214721016585827,
0.019009016454219818,
-0.041831448674201965,
0.09627440571784973,
-0.006154820788651705,
-0.05310270935297012,
0.0362093523144722,
0.003352650674059987,
-0.09616269171237946,
-0.012327948585152626,
0.0... |
PMID:20977 | Resonance Raman study of the pH-dependent and detergent-induced structural alterations in the heme moiety of Rhodospirillum rubrum cytochrome c'. | The resonance Raman spectra and the structures of the heme moiety of Rhodospirillum rubrum cytochrome c' were investigated for its five states characterized by absorption spectra; Types-a and -n of the reduced form and Types-I, -II, and -III of the oxidized form. The frequency of the ligand-sensitive Raman line suggest... | Resonance Raman study of the pH-dependent and detergent-induced structural alterations in the heme moiety of Rhodospirillum rubrum cytochrome c'. The resonance Raman spectra and the structures of the heme moiety of Rhodospirillum rubrum cytochrome c' were investigated for its five states characterized by absorption spe... | [
-0.10414382815361023,
0.0492018386721611,
-0.08543255925178528,
0.02825257182121277,
0.06803935766220093,
-0.042028140276670456,
0.046174027025699615,
0.008683972992002964,
-0.02849063277244568,
-0.023553742095828056,
0.04925210028886795,
-0.03881184384226799,
0.04997355863451958,
0.034633... |
PMID:20978 | Hydrogen ion titration and amino acid analysis of hemocyanin from the spiny lobster Jasus edwardsii. | Potentiometric and spectrophotometric titrations, isoelectric focusing and amino acid analyses, have been made on the hemocyanin from Jasus edwardsii. Counts of acidic neutral and alkaline groups were made from the titrations, enabling comparisons to be made with the amino acid analysis. Thermodynamic analysis of the d... | Hydrogen ion titration and amino acid analysis of hemocyanin from the spiny lobster Jasus edwardsii. Potentiometric and spectrophotometric titrations, isoelectric focusing and amino acid analyses, have been made on the hemocyanin from Jasus edwardsii. Counts of acidic neutral and alkaline groups were made from the titr... | [
-0.10242410004138947,
-0.04550249129533768,
-0.03465039283037186,
-0.04531051963567734,
0.006400285288691521,
-0.0319942943751812,
0.08190174400806427,
0.025090478360652924,
-0.07016932219266891,
-0.032458238303661346,
0.038259848952293396,
-0.05226503312587738,
0.023543719202280045,
0.032... |
PMID:20979 | Effect of tyrosine ionization upon biological activities of angiotensin II and two new peptide analogues. | The role of the tyrosine side-chain in the smooth muscle contracting activity of angiotensin III was investigated by determining intrinsic activities and ED50 values of [4-(3-chlorotyrosine)]angiotensin II and [4-(3-benzyltyrosine)]angiotensin II in the isolated guinea-pig ileum and rat uterus. [4-(3-chlorotyrosine)]an... | Effect of tyrosine ionization upon biological activities of angiotensin II and two new peptide analogues. The role of the tyrosine side-chain in the smooth muscle contracting activity of angiotensin III was investigated by determining intrinsic activities and ED50 values of [4-(3-chlorotyrosine)]angiotensin II and [4-(... | [
-0.0477493479847908,
-0.02437666244804859,
-0.07915148884057999,
0.03163781389594078,
0.025910871103405952,
-0.03063846193253994,
0.0567348450422287,
0.040726594626903534,
-0.03891007974743843,
-0.039497390389442444,
-0.00857105478644371,
-0.06225297600030899,
0.038564372807741165,
0.02370... |
PMID:20980 | Increased oxygen affinity for hemoglobin Sawara: alphaA4(6) aspartic acid replaced by alanine. | The oxygen binding property of Hb Sawara (alphaA4 Asp replaced by Ala) was studied at different pH values with and without addition of 2,3-diphosphoglycerate. The oxygen affinity of Hb Sawara was shown to be increased, the difference of the log P50 value between normal and abnormal hemoglobins being 0.37 at pH 7.0. Bot... | Increased oxygen affinity for hemoglobin Sawara: alphaA4(6) aspartic acid replaced by alanine. The oxygen binding property of Hb Sawara (alphaA4 Asp replaced by Ala) was studied at different pH values with and without addition of 2,3-diphosphoglycerate. The oxygen affinity of Hb Sawara was shown to be increased, the di... | [
-0.10660237818956375,
0.06479520350694656,
-0.02798677235841751,
0.04028475284576416,
-0.02141007035970688,
-0.030254943296313286,
0.08517857640981674,
0.01342781726270914,
-0.09082835167646408,
0.022691646590828896,
0.055200282484292984,
-0.10232128947973251,
-0.015481250360608101,
0.0573... |
PMID:20982 | Thermodynamic study of the formation of adenine nucleotide-manganese complexes. I. "pH stat" titration method results. | The thermodynamics associated with the Mn2- -ATP, Mn-ADP- and Mn-AMP complex formation reactions determined from K potentiometric measurements at I = 0.2 are reported for the temperature range 1--45 degrees C. The K values increase with the length of the phosphate chain and with temperature. The limits and the best con... | Thermodynamic study of the formation of adenine nucleotide-manganese complexes. I. "pH stat" titration method results. The thermodynamics associated with the Mn2- -ATP, Mn-ADP- and Mn-AMP complex formation reactions determined from K potentiometric measurements at I = 0.2 are reported for the temperature range 1--45 de... | [
-0.08396431058645248,
0.025300515815615654,
0.009923890233039856,
-0.012317345477640629,
0.046901505440473557,
-0.007652804721146822,
0.058902353048324585,
0.00935317762196064,
-0.07172995060682297,
-0.06072169169783592,
0.05373920872807503,
-0.04332522675395012,
0.06668813526630402,
0.019... |
PMID:20983 | Thermodynamic study of the formation of adenine nucleotide-manganese complexes. II. Calorimetric results. | Enthalpic variations in the formation of adenine nucleotide-manganese complexes, as measured by microcalorimetry, are reported. All the results are obtained in the temperature range 6--30 degrees C at I =0.2 and pH values 7.00 or 7.50. All the reactions are endothermic and the deltaH values increase with the length of ... | Thermodynamic study of the formation of adenine nucleotide-manganese complexes. II. Calorimetric results. Enthalpic variations in the formation of adenine nucleotide-manganese complexes, as measured by microcalorimetry, are reported. All the results are obtained in the temperature range 6--30 degrees C at I =0.2 and pH... | [
-0.09061820060014725,
0.023757917806506157,
0.0035675805993378162,
0.0352117195725441,
0.059822242707014084,
0.009987362660467625,
0.027727650478482246,
0.02101891115307808,
-0.05712536349892616,
-0.05567239597439766,
0.041872669011354446,
-0.03499787300825119,
0.05004563927650452,
0.04516... |
PMID:20988 | [Isolation and characterization of beta-D-galactosidase from gastric juice]. | A method for beta-D-galactosidase isolation from cattle gastric juice has been developed. Gastric juice mucus was removed by and addition of equimolar amounts of Na2HPO4 and CaCl2. The removal of proteases and other proteins was achieved by the treatment with resins KB-51X2 and AN-22. The resulting preparation had spec... | [Isolation and characterization of beta-D-galactosidase from gastric juice]. A method for beta-D-galactosidase isolation from cattle gastric juice has been developed. Gastric juice mucus was removed by and addition of equimolar amounts of Na2HPO4 and CaCl2. The removal of proteases and other proteins was achieved by th... | [
-0.04368866607546806,
-0.02575651928782463,
0.017113903537392616,
-0.031983066350221634,
0.05420294776558876,
-0.06461463868618011,
0.014762361533939838,
0.06563951075077057,
-0.02797001786530018,
-0.0459989570081234,
-0.004851481411606073,
-0.07436301559209824,
-0.0732048973441124,
0.0065... |
PMID:20989 | [Kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase]. | A kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the pH range of 3.7-9.0 has been carried out. It was shown that the reaction of o-dianisidine peroxidase oxidation obeys the Michaelis--Menten kinetics; the kcat and Km values within the pH range used were d... | [Kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase]. A kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the pH range of 3.7-9.0 has been carried out. It was shown that the reaction of o-dianisidine peroxi... | [
-0.09350261092185974,
0.004426109604537487,
-0.024484822526574135,
0.015544632449746132,
0.029683396220207214,
-0.07642429322004318,
-0.006323578301817179,
0.03011947125196457,
-0.04631568491458893,
-0.023727040737867355,
0.020256416872143745,
0.010488233529031277,
-0.05194837599992752,
0.... |
PMID:20990 | [Purification and some properties of isozymes 1 and 5 of lactate dehydrogenase from fox heart and skeletal muscles]. | An improved method is described for the isolation of isozymes 1 and 5 of lactate dehydrogenase (LDH) from heart and skeletal muscles of foxes. The method includes salt fractionation with ammonium sulphate, chromatography on DEAE- and CM-celluloses and affinity chromatography on AMP-Sepharose. The preparations of LDH i... | [Purification and some properties of isozymes 1 and 5 of lactate dehydrogenase from fox heart and skeletal muscles]. An improved method is described for the isolation of isozymes 1 and 5 of lactate dehydrogenase (LDH) from heart and skeletal muscles of foxes. The method includes salt fractionation with ammonium sulpha... | [
-0.031188135966658592,
-0.016598187386989594,
-0.03174331411719322,
0.08247777819633484,
0.008685438893735409,
-0.05416485667228699,
0.016182390972971916,
0.0350295715034008,
-0.03783624991774559,
-0.004512065090239048,
0.03660598769783974,
-0.06859524548053741,
0.0014779564226046205,
0.03... |
PMID:20986 | [Photogeneration of a 2-vector transmembrane proton gradient in Halobacterium halobium R1 cells]. | Analysing 4 phases of light-dependent change of pH in cell suspension of H. halobium R1 it has been found that an increase of pH I when light is switched on and a decrease of pH II during further illumination are proportional to the light effect and are energy-dependent. Neutralization of these changes (phases III and ... | [Photogeneration of a 2-vector transmembrane proton gradient in Halobacterium halobium R1 cells]. Analysing 4 phases of light-dependent change of pH in cell suspension of H. halobium R1 it has been found that an increase of pH I when light is switched on and a decrease of pH II during further illumination are proportio... | [
-0.09507884085178375,
0.019558260217308998,
-0.027694186195731163,
0.051277145743370056,
0.03189318627119064,
0.008008245378732681,
0.05839816480875015,
0.005616206210106611,
-0.01996929571032524,
0.0433775931596756,
0.054667167365550995,
-0.09732215106487274,
0.00031547588878311217,
0.038... |
PMID:20991 | [Comparative study of NADP-reductase properties in two species of purple bacteria]. | Unlike Rhodospirillum rubrum, the highly purified preparations of NADP-reductase Thiocapsa roseopersicina are capable of reduction of cytochrome c though they do not catalyse diaphorase reaction in the presence of methyl viologen or benzyl viologen and NADH. T. roseopersicina reductase has more high temperature optimum... | [Comparative study of NADP-reductase properties in two species of purple bacteria]. Unlike Rhodospirillum rubrum, the highly purified preparations of NADP-reductase Thiocapsa roseopersicina are capable of reduction of cytochrome c though they do not catalyse diaphorase reaction in the presence of methyl viologen or ben... | [
-0.0826539471745491,
-0.012004571035504341,
-0.024882357567548752,
0.04800352081656456,
0.05151022970676422,
-0.031717170029878616,
0.04235409200191498,
0.04086018726229668,
-0.06183842569589615,
-0.03752869367599487,
0.007251343689858913,
-0.043523259460926056,
0.022504957392811775,
-0.00... |
PMID:20987 | [Functional role of parvalbumins in regulating Ca2+ in the contraction--relaxation cycle of vertebrate skeletal muscles]. | On the basis of parvalbumin property to change Ca2+-binding constant within the pH-region from approxim-tely 7 to approximately 8 a hypothesis that parvalbumins are pH-regulated Ca2+-depot in muscles has been stated. The addition of parvalbumin to native actomysin complex at pH approximately 7 in the presence of ATP an... | [Functional role of parvalbumins in regulating Ca2+ in the contraction--relaxation cycle of vertebrate skeletal muscles]. On the basis of parvalbumin property to change Ca2+-binding constant within the pH-region from approxim-tely 7 to approximately 8 a hypothesis that parvalbumins are pH-regulated Ca2+-depot in muscle... | [
-0.0714106634259224,
-0.06318861991167068,
-0.031232276931405067,
-0.037135049700737,
0.01729741320014,
-0.036368533968925476,
0.05704303830862045,
-0.000625789980404079,
-0.011918362230062485,
0.021918565034866333,
0.008177212439477444,
-0.09115642309188843,
0.021634940057992935,
0.058258... |
PMID:20992 | [Virioimmunoassay for studying the interaction of diphtheria toxin with cell membranes]. | A model for studying the interaction of diphtheria toxin with cell membranes includes immobilization of purified cell membranes on Sephadex G-25, adsorbtion of toxin on the membranes in the presence of protective colloid, and subsequent detection of adsorbed toxin by means of virioimmunoassay. Diphtheria toxin adsorbed... | [Virioimmunoassay for studying the interaction of diphtheria toxin with cell membranes]. A model for studying the interaction of diphtheria toxin with cell membranes includes immobilization of purified cell membranes on Sephadex G-25, adsorbtion of toxin on the membranes in the presence of protective colloid, and subse... | [
-0.05511388182640076,
0.024339566007256508,
-0.04143102094531059,
0.04791506752371788,
-0.00016183385741896927,
-0.0479353703558445,
0.09486642479896545,
0.05609482154250145,
-0.05474292114377022,
-0.017626183107495308,
-0.024998117238283157,
-0.06375911831855774,
0.022229140624403954,
0.0... |
PMID:20993 | [Precipitation of neutral alpha-glucans and separation of mixtures by dimethyldodecylbenzylammonium chloride]. | The solubilization of precipitated complex of alpha-polyglucane-dimethyldodecylbenzylammonium chloride in excess of a solution of the salt was found to be dependent on pH. The complexes of glycogen having a more branching structure are dissolved much more readily than the amylopectin complexes. The conditions for separ... | [Precipitation of neutral alpha-glucans and separation of mixtures by dimethyldodecylbenzylammonium chloride]. The solubilization of precipitated complex of alpha-polyglucane-dimethyldodecylbenzylammonium chloride in excess of a solution of the salt was found to be dependent on pH. The complexes of glycogen having a mo... | [
-0.05644974485039711,
-0.0015153991989791393,
0.016928061842918396,
-0.03399433568120003,
0.02060892805457115,
-0.06538587063550949,
0.020761385560035706,
-0.0012546316720545292,
-0.0421786792576313,
-0.06620413064956665,
-0.013489334844052792,
-0.08038206398487091,
0.0003934699925594032,
... |
PMID:20994 | [Regulation of Na, K-ATPase activity by monovalent cations]. | A deviation from optimal conditions of the Na, K-ATPase reaction results in a drastic change in the plot: enzyme activity versus Na/K ratio. Acidification of the medium and a decrease in Mg2+ concentration and temperature results in two peaks on the curve at Na/K ratio of about 1 and at Na/K ratio greater than 4. The e... | [Regulation of Na, K-ATPase activity by monovalent cations]. A deviation from optimal conditions of the Na, K-ATPase reaction results in a drastic change in the plot: enzyme activity versus Na/K ratio. Acidification of the medium and a decrease in Mg2+ concentration and temperature results in two peaks on the curve at ... | [
-0.06682558357715607,
-0.013344850391149521,
0.005849743261933327,
0.02568693831562996,
0.05908999964594841,
-0.0321342796087265,
0.041775982826948166,
0.04601738229393959,
-0.03177124261856079,
-0.01752369850873947,
0.02611555904150009,
-0.036783769726753235,
-0.01794831082224846,
0.07907... |
PMID:20995 | [Reversible dissociation of malate dehydrogenase from plants]. | It was demonstrated that 0.2 M citric acid (pH 2.5) inactivates highly-purified malate dehydrogenase from tea leaves; the degree of inactivation depends on temperature and time of incubation. The enzyme activity is restored by certain inorganic salts, the degree of reactivation being dependent on pH, ionic strengths of... | [Reversible dissociation of malate dehydrogenase from plants]. It was demonstrated that 0.2 M citric acid (pH 2.5) inactivates highly-purified malate dehydrogenase from tea leaves; the degree of inactivation depends on temperature and time of incubation. The enzyme activity is restored by certain inorganic salts, the d... | [
-0.01610364206135273,
-0.02104530669748783,
-0.01831815205514431,
0.04661686345934868,
0.03369396924972534,
-0.0014611927326768637,
0.07947102934122086,
0.033859215676784515,
-0.03541320562362671,
-0.04573647305369377,
0.03355566784739494,
-0.07261571288108826,
0.015037809498608112,
0.0191... |
PMID:20996 | [Effects of nucleophils on kinetic parameters of peroxidase-catalyzed oxidative reactions]. | The effect of imidazole on the kinetic parameters of reactions of potassium ferrocyanide and o-dianizidine peroxidation by hydrogen peroxide within a wide range of pH was studied. It was shown that imidazole activates the reaction of o-dianizidine peroxidation at pH greater than or equal to 6.5, but does not affect the... | [Effects of nucleophils on kinetic parameters of peroxidase-catalyzed oxidative reactions]. The effect of imidazole on the kinetic parameters of reactions of potassium ferrocyanide and o-dianizidine peroxidation by hydrogen peroxide within a wide range of pH was studied. It was shown that imidazole activates the reacti... | [
-0.09756433963775635,
0.003315581241622567,
-0.04280068725347519,
0.003011167049407959,
0.049980394542217255,
-0.028380343690514565,
0.032966434955596924,
0.10255911946296692,
-0.06348612904548645,
-0.0370609425008297,
0.006298414897173643,
0.032428111881017685,
0.005604420322924852,
0.047... |
PMID:20997 | [Dependence of thrombin- and trypsin-catalyzed hydrolysis of N-alpha-arylsulfonyl-L-arginine methyl esters on the structure of acylamide part of substrates]. | For comparative studies on the esterase activities of thrombin and trypsin N(alpha)-arylsulfonyl-L-arginine methyl esters were synthetised containing in aromatic ring substituents of different polar nature, size and hydrophobicity. The kinetics of their hydrolysis by thrombin and trypsin were measured. Values of Km and... | [Dependence of thrombin- and trypsin-catalyzed hydrolysis of N-alpha-arylsulfonyl-L-arginine methyl esters on the structure of acylamide part of substrates]. For comparative studies on the esterase activities of thrombin and trypsin N(alpha)-arylsulfonyl-L-arginine methyl esters were synthetised containing in aromatic ... | [
-0.10590540617704391,
-0.014046705327928066,
-0.10076964646577835,
0.015398585237562656,
0.04042857512831688,
0.004300592001527548,
0.03822862729430199,
0.042568664997816086,
-0.04042579606175423,
-0.00622418662533164,
-0.01781664602458477,
-0.0081089548766613,
-0.024265993386507034,
0.031... |
PMID:20998 | [Characterization of dextranase from Penicillium purpurogenum (Ftoll)]. | An extracellular dextranase (E. C. 3.2.1.11) was purified from cell-free culture filtrates of Penicillium purpurogenum (Ftoll). The enzyme was most active at pH 5,5. The dextranase was endo-type, it split quickly isomaltotetraose into two isomaltose molecules, slowly degraded isomaltotriose, and did not act on isomalto... | [Characterization of dextranase from Penicillium purpurogenum (Ftoll)]. An extracellular dextranase (E. C. 3.2.1.11) was purified from cell-free culture filtrates of Penicillium purpurogenum (Ftoll). The enzyme was most active at pH 5,5. The dextranase was endo-type, it split quickly isomaltotetraose into two isomaltos... | [
-0.010333342477679253,
-0.027080081403255463,
-0.02279379405081272,
-0.00011768138938350603,
0.029024407267570496,
-0.023706384003162384,
0.011822842992842197,
0.03763005509972572,
-0.029361261054873466,
0.012529943138360977,
0.01942993514239788,
-0.08805842697620392,
-0.0022787614725530148,... |
PMID:20999 | [Effect of environment on allosteric properties of acetylcholinesterase]. | In the presence of organophosphorus inhibitors (OPI) AChE inhibition is initiated at a lower concentration of ACh; the plot reaction rate versus substrate concentration shows two maxima with a distinct minimum between them. It was shown that extremely mild conditions (short-term heating up to 50 degrees C; acidic or al... | [Effect of environment on allosteric properties of acetylcholinesterase]. In the presence of organophosphorus inhibitors (OPI) AChE inhibition is initiated at a lower concentration of ACh; the plot reaction rate versus substrate concentration shows two maxima with a distinct minimum between them. It was shown that extr... | [
-0.036595817655324936,
0.04931267723441124,
0.006927977781742811,
0.06731396168470383,
-0.019922936335206032,
-0.030775876715779305,
0.06845316290855408,
0.036996323615312576,
-0.04953343793749809,
-0.03695220872759819,
0.01304794941097498,
-0.0029353236313909292,
0.03407527878880501,
0.08... |
PMID:21000 | [Proteins of Bacillus thuringiensis delta-endotoxin crystals]. | Pure crystals (at least 99% purification) of sigma-endotoxin were isolated from Bac. thuringiensis var. galleriae. The complete dissolution of crystals might be achieved by the increase of pH up to 12 and higher or by a combined action of S = S-reducing and denaturing agents. Electrophoresis of the solubilized crystal ... | [Proteins of Bacillus thuringiensis delta-endotoxin crystals]. Pure crystals (at least 99% purification) of sigma-endotoxin were isolated from Bac. thuringiensis var. galleriae. The complete dissolution of crystals might be achieved by the increase of pH up to 12 and higher or by a combined action of S = S-reducing and... | [
-0.053593624383211136,
-0.03284715861082077,
-0.012268458493053913,
0.004104943480342627,
0.04199903458356857,
-0.0241719800978899,
0.056962400674819946,
0.046882256865501404,
-0.07498972862958908,
0.01331985555589199,
-0.018483895808458328,
-0.1050756499171257,
0.04425576701760292,
0.0516... |
PMID:21004 | The gas chromatography mass spectrometry of the major metabolites of flurazepam. | Mass spectra and gas chromatographic data are presented for flurazepam and its metabolites; monodesethylflurazepam, didesethylflurazepam, hydroxyethylflurazepam, N1-desalkylflurazepam, N1-desalkyl-3-hydroxy-flurazepam, and flurazepam-N1-acetic acid. The on-column thermal degradation of didesethylflurazepam, N1-desalkyl... | The gas chromatography mass spectrometry of the major metabolites of flurazepam. Mass spectra and gas chromatographic data are presented for flurazepam and its metabolites; monodesethylflurazepam, didesethylflurazepam, hydroxyethylflurazepam, N1-desalkylflurazepam, N1-desalkyl-3-hydroxy-flurazepam, and flurazepam-N1-ac... | [
-0.005094716791063547,
-0.008016171865165234,
0.02125541679561138,
0.019141897559165955,
0.04658767580986023,
0.012783809565007687,
-0.003451415803283453,
0.013580238446593285,
-0.012486480176448822,
-0.06727474927902222,
0.006371327675879002,
-0.01497388631105423,
-0.02046949788928032,
0.... |
PMID:21006 | [Changes in the electrical activity of myocardial fibers following heart transplantation to under the skin of the ear in mice]. | Intracellular rest and action potentials (RP and AP, respectively) of mouse heart graft were reduced on the 3rd--4th days after the transplantation as compared with the intracellular potentials of newborn mice. Beginning from the 5th--6th days there occurred a gradual increase of the intracellular potentials of amplitu... | [Changes in the electrical activity of myocardial fibers following heart transplantation to under the skin of the ear in mice]. Intracellular rest and action potentials (RP and AP, respectively) of mouse heart graft were reduced on the 3rd--4th days after the transplantation as compared with the intracellular potential... | [
-0.04845363646745682,
-0.045987918972969055,
0.02211163006722927,
0.04970315471291542,
-0.014537057839334011,
-0.03794267773628235,
0.05611703544855118,
0.02902320772409439,
-0.01922839879989624,
0.005613483488559723,
0.02848142199218273,
-0.07076528668403625,
-0.052469003945589066,
-0.049... |
PMID:21007 | [Inhibitory processes in the cerebral cortex and the anticonvulsive action of benzodiazepine derivatives]. | A comparative study of the effect of some benzodiazepine deprivatives (chlonazepam, lorazepam, diazepam, and medazepam) on the recovery cycles of the interzonal response was carried out on unanesthetized curare-immobilized cats. These drugs proved to selectively inhibit the testing potential within the range of 20 to 1... | [Inhibitory processes in the cerebral cortex and the anticonvulsive action of benzodiazepine derivatives]. A comparative study of the effect of some benzodiazepine deprivatives (chlonazepam, lorazepam, diazepam, and medazepam) on the recovery cycles of the interzonal response was carried out on unanesthetized curare-im... | [
-0.07903104275465012,
0.022696884348988533,
0.017139671370387077,
0.07454376667737961,
-0.005124463699758053,
0.04287188872694969,
0.08095259964466095,
0.06443648040294647,
-0.0318218432366848,
-0.02988814376294613,
-0.02413242869079113,
0.015053380280733109,
-0.002579412655904889,
0.02970... |
PMID:21008 | [Changes in the H-alloantigen-recognizing function of mouse lymphoid cells following hydrocortisone administration]. | The capacity of spleen, thymus, and bone marrow cells of intact (control) and of hydrocortisone-treated mice CBA to induce the lymph node type of graft-v-host reaction (GVHR) in hybrids F1 (CBA X c57bl) was studied. After hydrocortisone injection (2.5 mg per mouse) the donor spleen cells became more active in GVHR, con... | [Changes in the H-alloantigen-recognizing function of mouse lymphoid cells following hydrocortisone administration]. The capacity of spleen, thymus, and bone marrow cells of intact (control) and of hydrocortisone-treated mice CBA to induce the lymph node type of graft-v-host reaction (GVHR) in hybrids F1 (CBA X c57bl) ... | [
-0.010781912133097649,
0.021902715787291527,
-0.04982094466686249,
0.022859230637550354,
0.01666642725467682,
-0.04965192452073097,
0.04338475689291954,
0.06496281921863556,
-0.04231398180127144,
0.009314660914242268,
0.02569919265806675,
-0.023987330496311188,
-0.0005891570472158492,
-0.0... |
PMID:21009 | [Effect of aminazin on the enzymatic oxidation of lipids]. | An inhibitory effect of chlorpromazine on the enzymatic NADPH-dependent lipid peroxidation in rat liver microsomal fraction was found. This inhibition was caused by the 1) antioxidative effect of hydroxy-derivatives appearing during the oxidative metabolism of chlorpromazine with NADPH-dependent microsomal oxygenases, ... | [Effect of aminazin on the enzymatic oxidation of lipids]. An inhibitory effect of chlorpromazine on the enzymatic NADPH-dependent lipid peroxidation in rat liver microsomal fraction was found. This inhibition was caused by the 1) antioxidative effect of hydroxy-derivatives appearing during the oxidative metabolism of ... | [
-0.09303803741931915,
0.06833438575267792,
-0.05420267954468727,
0.07908396422863007,
-0.0015186979435384274,
0.010618654079735279,
0.06007429584860802,
0.035630881786346436,
-0.0524214543402195,
-0.05982952564954758,
-0.0015846024034544826,
0.037845075130462646,
0.0593402236700058,
0.0418... |
PMID:21013 | The dependence of the lipid bilayer membrane: buffer partition coefficient of pentobarbitone on pH and lipid composition. | 1 The membrane/buffer partition coefficient of [14C]-pentobarbitone has been determined as a function of the lipid composition of bilayer membranes. 2 A new technique based on ultrafiltration gave comparable results to conventional techniques but required less time for equilbration. 3 The membrane/buffer coefficient wa... | The dependence of the lipid bilayer membrane: buffer partition coefficient of pentobarbitone on pH and lipid composition. 1 The membrane/buffer partition coefficient of [14C]-pentobarbitone has been determined as a function of the lipid composition of bilayer membranes. 2 A new technique based on ultrafiltration gave c... | [
-0.04906086251139641,
-0.023835955187678337,
0.018851416185498238,
-0.025808461010456085,
-0.03316755220293999,
-0.03724087402224541,
0.08262139558792114,
0.05869000777602196,
-0.0013859588652849197,
-0.029766833409667015,
0.005175958387553692,
-0.09441811591386795,
0.02974105440080166,
0.... |
PMID:21014 | Assessment of the selectivity of OPC-2009, a new beta2-adrenoceptor stimulatn, by the use of the blood-perfused trachea in situ and of the isolated blood-perfused papillary muscle of the dog. | 1 The potency and selectivity of 5-(1-hydroxy-2-isopropylamino)butyl-8-hydroxy carbostyril hydrochloride hemihydrate (OPC-2009), a new beta(2)-adrenoceptor stimulant, was compared with those of isoprenaline, trimetoquinol and salbutamol by the use of blood-perfused tracheal preparations in situ and of blood-perfused pa... | Assessment of the selectivity of OPC-2009, a new beta2-adrenoceptor stimulatn, by the use of the blood-perfused trachea in situ and of the isolated blood-perfused papillary muscle of the dog. 1 The potency and selectivity of 5-(1-hydroxy-2-isopropylamino)butyl-8-hydroxy carbostyril hydrochloride hemihydrate (OPC-2009),... | [
-0.053883522748947144,
-0.0340116061270237,
-0.05150255560874939,
0.010774069465696812,
-0.008133410476148129,
0.041075851768255234,
0.043452516198158264,
0.03673739358782768,
-0.01939835399389267,
-0.0008187115308828652,
-0.015233735553920269,
-0.02052946388721466,
-0.040362536907196045,
... |
PMID:21015 | Attitude change during behavioural treatment of sexual inadequacy. | Attitudes towards 'self' and 'partner' were studied in couples undergoing three different behavioural treatments for sexual inadequacy: systematic desensitization with counselling; guided practice with counselling; and practice with minimal counselling. Factor analysis of semantic differential scales identified five co... | Attitude change during behavioural treatment of sexual inadequacy. Attitudes towards 'self' and 'partner' were studied in couples undergoing three different behavioural treatments for sexual inadequacy: systematic desensitization with counselling; guided practice with counselling; and practice with minimal counselling.... | [
0.028496500104665756,
0.054796721786260605,
0.012469637207686901,
-0.003926314879208803,
-0.0019178596558049321,
0.0734315738081932,
0.060731589794158936,
-0.007104236166924238,
0.0639997273683548,
-0.06618247181177139,
0.030465135350823402,
-0.0533568300306797,
0.042459581047296524,
0.014... |
PMID:21018 | Histochemical and biochemical characterization of the rat paracervical ganglion. | The nature and identity of the catecholamines in the paracervical ganglion and superior cervical ganglion small, intensely fluorescent (SIF) cells were investigated using fluorescence histochemical and immunohistochemical techniques. The paracervical ganglion SIF cells were found to contain norepinephrine and the super... | Histochemical and biochemical characterization of the rat paracervical ganglion. The nature and identity of the catecholamines in the paracervical ganglion and superior cervical ganglion small, intensely fluorescent (SIF) cells were investigated using fluorescence histochemical and immunohistochemical techniques. The p... | [
0.01519953552633524,
-0.009696866385638714,
-0.021248113363981247,
0.006688522174954414,
0.02415180392563343,
0.048740871250629425,
0.060065414756536484,
0.03515521064400673,
0.010897851549088955,
-0.05208560451865196,
0.015647737309336662,
-0.042122732847929,
-0.01336644683033228,
0.06240... |
PMID:21022 | [Guanyl cyclase activity in the EF-T elongation factor of Escherichia coli]. | Highly purified EF-Ts from E. coli does contain guanylate cyclase activity, which is absent from other purified transfer factors, such as EF-Tu and EF-G. Guanylate cyclase activity has been characterized by its sensitivity to inhibitors and substrate specificity. Although the physicochemical properties of guanylate cyc... | [Guanyl cyclase activity in the EF-T elongation factor of Escherichia coli]. Highly purified EF-Ts from E. coli does contain guanylate cyclase activity, which is absent from other purified transfer factors, such as EF-Tu and EF-G. Guanylate cyclase activity has been characterized by its sensitivity to inhibitors and su... | [
-0.05870490521192551,
-0.008263777941465378,
-0.013469413854181767,
0.0025451574474573135,
0.0852307677268982,
-0.051259979605674744,
0.0024948655627667904,
0.021511128172278404,
-0.046999480575323105,
0.026898276060819626,
-0.0054726265370845795,
-0.06613493710756302,
0.0016316681867465377,... |
PMID:21021 | Relationship between urine acidification and intracellular pH in respiratory acidosis. | The renal net acid excretion (NAE), the blood pH (pHe), the total body intracellular pH (pHi) and the urinary pH (pHu) were calculated in 10 patients with chronic obstructive lung disease and hypercapnia and in 5 normocapnic subjects. The mean value of NAE was significantly higher in hypercapnic subjects than in normoc... | Relationship between urine acidification and intracellular pH in respiratory acidosis. The renal net acid excretion (NAE), the blood pH (pHe), the total body intracellular pH (pHi) and the urinary pH (pHu) were calculated in 10 patients with chronic obstructive lung disease and hypercapnia and in 5 normocapnic subjects... | [
-0.01916550286114216,
0.014493421651422977,
0.02408624067902565,
0.017863178625702858,
0.036757394671440125,
-0.000217079883441329,
0.13319845497608185,
0.0320577546954155,
0.023461274802684784,
-0.011304852552711964,
-0.030698567628860474,
-0.09241054207086563,
0.07078014314174652,
0.0458... |
PMID:21027 | Optimum pH and ionic strength for the assay of cytochrome c oxidase from pea cotyledon mitochondria. | Cytochrome c oxidase (EC 1.9.3.1) has been solubilized by use of the nonionic detergents were determined for the oxidation of ferrocytochrome c in air. The results indicate that the plant cytochrome c oxidase resembles mammalian preparations in its sensitivity towards ionic strength and pH of the assay buffer. | Optimum pH and ionic strength for the assay of cytochrome c oxidase from pea cotyledon mitochondria. Cytochrome c oxidase (EC 1.9.3.1) has been solubilized by use of the nonionic detergents were determined for the oxidation of ferrocytochrome c in air. The results indicate that the plant cytochrome c oxidase resembles ... | [
-0.044991061091423035,
-0.016290854662656784,
-0.06469269096851349,
0.01465720683336258,
0.05045880749821663,
-0.059869762510061264,
0.02651667781174183,
0.031476523727178574,
-0.04057108983397484,
0.02442190982401371,
0.01431394461542368,
-0.051442667841911316,
0.0049514868296682835,
0.05... |
PMID:21028 | Effect of metabolic alkalosis on respiratory function in patients with chronic obstructive lung disease. | Eleven instances of a mixed acid-base disorder consisting of chronic respiratory acidosis and metabolic alkalosis were recognized in eight patients with chronic obstructive lung disease and carbon dioxide retention. Correction of the metabolic alkalosis led to substantial improvement in blood gas values and clinical sy... | Effect of metabolic alkalosis on respiratory function in patients with chronic obstructive lung disease. Eleven instances of a mixed acid-base disorder consisting of chronic respiratory acidosis and metabolic alkalosis were recognized in eight patients with chronic obstructive lung disease and carbon dioxide retention.... | [
-0.03206736594438553,
0.015486028976738453,
0.02805759198963642,
0.06770827621221542,
-0.026001328602433205,
-0.009306739084422588,
0.04904640093445778,
-0.022206122055649757,
-0.044160209596157074,
0.038959234952926636,
0.019435085356235504,
-0.08264144510030746,
0.0013991349842399359,
0.... |
PMID:21030 | Structural studies on the specific type-14 pneumococcal polysaccharide. | The structure of the Pneumococcus type-14 capsular polysaccharide has been reinvestigated by using methylation analysis, different specific degradations, and n.m.r. spectroscopy. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the structure: (formula: see text). | Structural studies on the specific type-14 pneumococcal polysaccharide. The structure of the Pneumococcus type-14 capsular polysaccharide has been reinvestigated by using methylation analysis, different specific degradations, and n.m.r. spectroscopy. It is concluded that the polysaccharide is composed of tetrasaccharid... | [
-0.06230267509818077,
-0.0012881819857284427,
-0.06829793006181717,
-0.040074095129966736,
0.032164108008146286,
0.010375261306762695,
0.002711755922064185,
-0.012054294347763062,
-0.021734321489930153,
-0.0044576567597687244,
0.019196422770619392,
-0.12203221768140793,
0.0061784349381923676... |
PMID:21032 | Left internal mammary--left ventricular fistula after Vineberg operation. | This communication presents an unusual complication in a patient who underwent the Vineberg procedure with the formation of an internal mammary to left-ventricular fistula, which caused a new apical diastolic murmur. This represents a previously unreported etiology for the appearance of an apical blowing diastolic murm... | Left internal mammary--left ventricular fistula after Vineberg operation. This communication presents an unusual complication in a patient who underwent the Vineberg procedure with the formation of an internal mammary to left-ventricular fistula, which caused a new apical diastolic murmur. This represents a previously ... | [
0.024000301957130432,
-0.03587951138615608,
-0.016352349892258644,
0.016332175582647324,
-0.0038905709516257048,
-0.03991725295782089,
0.012447905726730824,
0.11266669631004333,
0.04464828968048096,
0.00718568405136466,
0.01681373454630375,
-0.075632244348526,
-0.03893163427710533,
-0.0453... |
PMID:21038 | Clinical usefulness of alkaline phosphatase isoenzyme determinations. | 1. We report on the clinical usefulness of alkaline phosphatase isoenzyme determinations using a combined chemical inhibition method on 731 patient serum specimens exhibiting elevated (greater than 350 U/L) alkaline phosphatase (AP) activity. 2. The relative percentages of the organ-specific alkaline phosphatase activi... | Clinical usefulness of alkaline phosphatase isoenzyme determinations. 1. We report on the clinical usefulness of alkaline phosphatase isoenzyme determinations using a combined chemical inhibition method on 731 patient serum specimens exhibiting elevated (greater than 350 U/L) alkaline phosphatase (AP) activity. 2. The ... | [
0.006433968432247639,
-0.0010076310718432069,
-0.0008513773209415376,
0.013854078948497772,
-0.017401203513145447,
-0.03850061818957329,
0.0766390934586525,
0.07661372423171997,
-0.035601187497377396,
-0.023384789004921913,
-0.0023726411163806915,
-0.05861331894993782,
-0.030007556080818176,... |
PMID:21039 | Questionable usefulness of gamma-glutamyl transpeptidase test in legal medicine. | Because of the high level of GGT activity in semen, the suggestion was made that this enzyme could serve as a test in forensic medical practice; especially in rape cases. Comparisons of GGT and ACP tests revealed, however, that GGT is not sensitive enough and did not show acceptable specificity. One can conclude that G... | Questionable usefulness of gamma-glutamyl transpeptidase test in legal medicine. Because of the high level of GGT activity in semen, the suggestion was made that this enzyme could serve as a test in forensic medical practice; especially in rape cases. Comparisons of GGT and ACP tests revealed, however, that GGT is not ... | [
0.03743978962302208,
0.028133392333984375,
-0.04883280768990517,
-0.007243047468364239,
0.03985816240310669,
-0.0242698285728693,
0.07338302582502365,
0.05383174866437912,
-0.03797343000769615,
-0.042715057730674744,
-0.04677766188979149,
-0.03012038581073284,
-0.03273143619298935,
0.09659... |
PMID:21036 | [Enzymatic and serologic study of human trichinosis. Apropos of a recent epidemic in a suburb south of Paris]. | An enzymatic and immunologic study of 18 patients with trichinosis leads to the following conclusions: The stage of muscular invasion in trichinosis is accompanied by a release of cellular enzymes representative of striated muscle fibres in nearly all the cases. This release can be observed by a study of the LDH iso-en... | [Enzymatic and serologic study of human trichinosis. Apropos of a recent epidemic in a suburb south of Paris]. An enzymatic and immunologic study of 18 patients with trichinosis leads to the following conclusions: The stage of muscular invasion in trichinosis is accompanied by a release of cellular enzymes representati... | [
0.015122860670089722,
-0.0492062084376812,
-0.016800351440906525,
0.04992412030696869,
-0.0055065457709133625,
-0.09035896509885788,
0.05692053213715553,
0.054245442152023315,
-0.0015565467765554786,
-0.035861849784851074,
0.03779442980885506,
-0.06032344698905945,
-0.011478975415229797,
0... |
PMID:21040 | Use of different chemical methods for acid phosphatase in cases of rape. | Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's met... | Use of different chemical methods for acid phosphatase in cases of rape. Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's ... | [
-0.011844326741993427,
0.04656215012073517,
-0.04731353744864464,
0.04808333143591881,
0.001707469578832388,
-0.020242370665073395,
0.09798552840948105,
0.040571991354227066,
-0.025122134014964104,
-0.08187031745910645,
0.06100176274776459,
-0.09051603823900223,
-0.0038329933304339647,
0.0... |
PMID:21041 | Value of alkaline phosphatase, 5'-nucleotidase, gamma-glutamyltransferase, and glutamate dehydrogenase activity measurements (single and combined) in serum in diagnosis of metastasis to the liver. | We assessed, in 98 patients with cancer, the diagnostic value of measuring serum alkaline phosphatase, 5'-nucleotidase, gamma-glutamyltransferase, and glutamate dehydrogenase activities as an aid to detection of liver metastases. All four enzymes showed diagnostic value, but 5'-nucleotidase appeared to have the greates... | Value of alkaline phosphatase, 5'-nucleotidase, gamma-glutamyltransferase, and glutamate dehydrogenase activity measurements (single and combined) in serum in diagnosis of metastasis to the liver. We assessed, in 98 patients with cancer, the diagnostic value of measuring serum alkaline phosphatase, 5'-nucleotidase, gam... | [
0.0035247397609055042,
-0.016901770606637,
-0.027665913105010986,
-0.005472974386066198,
0.02836325205862522,
-0.036539532244205475,
0.08247769623994827,
0.03754657134413719,
-0.011237616650760174,
-0.040227051824331284,
0.07046373933553696,
-0.06304039061069489,
-0.01480635441839695,
-0.0... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.