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PMID:30039 | Mapping of the locus involved in the catabolic oxidation of D-amino acids in Pseudomonas aeruginosa PAO. | Mutants of Pseudomonas aeruginosa PAO deficient in their utilization of DL-valine have been found to have lost their capacity to utilize DL-alanine and L-proline. Use of conjugal and transductional mediated gene transfers have established the chromosomal location of this gene and also its pleotropic function in the ind... | Mapping of the locus involved in the catabolic oxidation of D-amino acids in Pseudomonas aeruginosa PAO. Mutants of Pseudomonas aeruginosa PAO deficient in their utilization of DL-valine have been found to have lost their capacity to utilize DL-alanine and L-proline. Use of conjugal and transductional mediated gene tra... | [
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PMID:30042 | [Fenfluramin (ponderax) intoxications in children (author's transl)]. | 4 cases of accidental fenfluramine poisoning in children are reported. Excitation, coma, convulsions, tachycardia, mydriasis, nystagmus, and rubeosis of the face were the most important signs. They appeared 30--60 min after ingestion, and some of them lasted for several days. 2 children had severe trismus and therefore... | [Fenfluramin (ponderax) intoxications in children (author's transl)]. 4 cases of accidental fenfluramine poisoning in children are reported. Excitation, coma, convulsions, tachycardia, mydriasis, nystagmus, and rubeosis of the face were the most important signs. They appeared 30--60 min after ingestion, and some of the... | [
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PMID:30043 | [Therapy with beta-blocking-agents (author's transl)]. | Within the last 10 years the indications for a therapeutic regimen with beta-blocking-agents have been differentiated: coronary heart disease with angina pectoris (interval regimen), essential hypertension, especially in younger persons; hyperkinetic heart syndrome; thyreotoxikosis, symptomatic therapy; heart rhythm di... | [Therapy with beta-blocking-agents (author's transl)]. Within the last 10 years the indications for a therapeutic regimen with beta-blocking-agents have been differentiated: coronary heart disease with angina pectoris (interval regimen), essential hypertension, especially in younger persons; hyperkinetic heart syndrome... | [
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PMID:30044 | Lymphocytic bronchitis associated with graft-versus-host disease in recipients of bone-marrow transplants. | Graft-versus-host disease, a complication of allogeneic bone-marrow transplantation, involves primarily the skin, liver and intestines, but may also be associated with pneumonia. To determine the relation of graft-versus-host disease with pneumonia, we evaluated the autopsies of 59 allogeneic and two autologous recipie... | Lymphocytic bronchitis associated with graft-versus-host disease in recipients of bone-marrow transplants. Graft-versus-host disease, a complication of allogeneic bone-marrow transplantation, involves primarily the skin, liver and intestines, but may also be associated with pneumonia. To determine the relation of graft... | [
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PMID:30046 | Steroid-induced meiotic division in Xenopus laevis oocytes: surface and calcium. | Progesterone reinitiates meiotic maturation in Xenopus oocytes. Evidence is reported which indicates that the steroid acts at the level of the cell surface and suggests that an induced change of Ca2+ distribution triggers in turn a cascade of cytoplasmic events including protein synthesis and germinal vesicle (nucleus)... | Steroid-induced meiotic division in Xenopus laevis oocytes: surface and calcium. Progesterone reinitiates meiotic maturation in Xenopus oocytes. Evidence is reported which indicates that the steroid acts at the level of the cell surface and suggests that an induced change of Ca2+ distribution triggers in turn a cascade... | [
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PMID:30047 | Benzodiazepines and central inhibitory mechanisms. | The effect of diazepam was evaluated on spontaneous activity and drug- and electrically-elicited inhibitions of neuronal activity. Doses of diazepam which did not change spontaneous firing rates markedly enhanced GABA-mediated inhibitions in rat cerebellum in situ and in tissue cultures of rat hypothalamus. The effects... | Benzodiazepines and central inhibitory mechanisms. The effect of diazepam was evaluated on spontaneous activity and drug- and electrically-elicited inhibitions of neuronal activity. Doses of diazepam which did not change spontaneous firing rates markedly enhanced GABA-mediated inhibitions in rat cerebellum in situ and ... | [
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PMID:30069 | Bohr effect induced by CO2 and fixed acid at various levels of O2 saturation in duck blood. | The Bohr factor, phi = delta log Po2/deltapH, was determined at various levels of hemoglobin O2 saturation (SO2) in fresh whole blood of the duck. Plasma pH was varied by either changing PCO2 of the blood at constant base excess (CO2 Bohr factor, phiCO2) or by addition of NaHCO3 and HCl at constant PCO2 (fixed acid BOH... | Bohr effect induced by CO2 and fixed acid at various levels of O2 saturation in duck blood. The Bohr factor, phi = delta log Po2/deltapH, was determined at various levels of hemoglobin O2 saturation (SO2) in fresh whole blood of the duck. Plasma pH was varied by either changing PCO2 of the blood at constant base excess... | [
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PMID:30070 | Carbon dioxide and acid base balance in the isolated rat diaphragm. | A method for measuring the net acid base exchange in an isolated rat diaphragm preparation is described. Particular attention is paid to monitoring the functional status and maintaining optimal diffusion conditions. A steady net acid efflux of the order of 250 n mole/g-min is found in the resting state. This increases ... | Carbon dioxide and acid base balance in the isolated rat diaphragm. A method for measuring the net acid base exchange in an isolated rat diaphragm preparation is described. Particular attention is paid to monitoring the functional status and maintaining optimal diffusion conditions. A steady net acid efflux of the orde... | [
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PMID:30083 | Modification of DNA by aflatoxin B1 creates alkali-labile lesions in DNA at positions of guanine and adenine. | The damage to DNA by the hepatocarcinogen aflatoxin B1 was investigated. A DNA fragment of known sequence of the lactose promoter-operator region was used as a substrate for modification by aflatoxin B1. The DNA was incubated with aflatoxin B1 in crude mammalian liver extracts or with purified microsomes. Treatment of ... | Modification of DNA by aflatoxin B1 creates alkali-labile lesions in DNA at positions of guanine and adenine. The damage to DNA by the hepatocarcinogen aflatoxin B1 was investigated. A DNA fragment of known sequence of the lactose promoter-operator region was used as a substrate for modification by aflatoxin B1. The DN... | [
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PMID:30084 | Functional lac carrier protein in cytoplasmic membrane vesicles isolated from Escherichia coli: temperature and pH dependence of dansyl-galactoside binding. | 6'-(N-Dansyl)aminohexyl-1-thio-beta-D-galactopyranoside binds specifically to the lac carrier protein in cytoplasmic membrane vesicles isolated from Escherichia coli. Binding can be induced by substrate oxidation (generation of an electrochemical gradient of protons), by potassium efflux in the presence of valinomycin ... | Functional lac carrier protein in cytoplasmic membrane vesicles isolated from Escherichia coli: temperature and pH dependence of dansyl-galactoside binding. 6'-(N-Dansyl)aminohexyl-1-thio-beta-D-galactopyranoside binds specifically to the lac carrier protein in cytoplasmic membrane vesicles isolated from Escherichia co... | [
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PMID:30085 | A macromolecular transducer as illustrated by trout hemoglobin IV. | Oxygen binding by trout Hb IV has been investigated as a function of pH up to 10 atmospheres (1 MPa) of pure O2. The results bring out an extreme proton-oxygen linkage, which gives rise to a Root effect. They are discussed in relation to the function of the hemoglobin as an oxygen pump. The system is of special interes... | A macromolecular transducer as illustrated by trout hemoglobin IV. Oxygen binding by trout Hb IV has been investigated as a function of pH up to 10 atmospheres (1 MPa) of pure O2. The results bring out an extreme proton-oxygen linkage, which gives rise to a Root effect. They are discussed in relation to the function of... | [
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PMID:30082 | [Multicomponent system of specific estrogen-binding liver proteins. Some properties of a rapidly dissociating estrogen-binding liver protein from guinea pigs]. | A study was made of some binding and physico-chemical properties of a special estrogen-binding protein of liver cytozol in sexually mature female and male guinea pigs following its partial purification by means of ammonium sulfate sedimentation, gelfiltration and ion-exchange chromatography. The given protein proved to... | [Multicomponent system of specific estrogen-binding liver proteins. Some properties of a rapidly dissociating estrogen-binding liver protein from guinea pigs]. A study was made of some binding and physico-chemical properties of a special estrogen-binding protein of liver cytozol in sexually mature female and male guine... | [
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PMID:30086 | Coated vesicles: characterization, selective dissociation, and reassembly. | Sodium dodecyl sulfate/polyacrylamide gels of coated vesicles from porcine brain (mean 76% coated vesicles) show three major proteins (180,000, 125,000, and 55,000 daltons) that account for 73% of the total protein. Preparations consisting predominantly of coats (65%) have less of the 55,000-dalton protein. Clathrin (1... | Coated vesicles: characterization, selective dissociation, and reassembly. Sodium dodecyl sulfate/polyacrylamide gels of coated vesicles from porcine brain (mean 76% coated vesicles) show three major proteins (180,000, 125,000, and 55,000 daltons) that account for 73% of the total protein. Preparations consisting predo... | [
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PMID:30087 | Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line. | We have established a clonal cell line, PC-G2, from an experimentally induced rat pheochromocytoma. Administration of nerve growth factor to PC-G2 causes a 4- to 8-fold induction in the specific activity of tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine,tetrahydropteridine:oxygen oxidoreductase(3-hydroxylat... | Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line. We have established a clonal cell line, PC-G2, from an experimentally induced rat pheochromocytoma. Administration of nerve growth factor to PC-G2 causes a 4- to 8-fold induction in the specific activity of tyrosine h... | [
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PMID:30092 | Kainic acid and synaptic transmission in the stellate ganglion of the squid. | Kainate, a conformational analogue of glutamate, blocks synaptic transmission across the giant synapse of the squid. In the presence of blocking doses of kainate, impulses continue to propagate into the nerve terminal, but action potentials are slightly reduced in size and the subsequent hyperpolarization is greatly di... | Kainic acid and synaptic transmission in the stellate ganglion of the squid. Kainate, a conformational analogue of glutamate, blocks synaptic transmission across the giant synapse of the squid. In the presence of blocking doses of kainate, impulses continue to propagate into the nerve terminal, but action potentials ar... | [
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PMID:30093 | Structural correlates of recurrent collateral interneurons producing both electrical and chemical inhibitions of the Mauthner cell. | Intracellular injections of horseradish peroxidase provided a basis for morphological identification of inhibitory interneurons belonging to the recurrent collateral network of the Mauthner cell. Their axons dilate to form unusually large bulbs surrounding the axon cap. The morphological appearance of these bulbs as we... | Structural correlates of recurrent collateral interneurons producing both electrical and chemical inhibitions of the Mauthner cell. Intracellular injections of horseradish peroxidase provided a basis for morphological identification of inhibitory interneurons belonging to the recurrent collateral network of the Mauthne... | [
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PMID:30095 | Brain catecholamines metabolism in offspring of amphetamine treated rats. | Previous observations have pointed out that treatment with amphetamine during pregnancy produces behavioral modifications in adulthood. In order to elucidate some possible brain biochemical mechanisms that could explain the behavioral changes observed we have determined the endogenous content of brain dopamine and nora... | Brain catecholamines metabolism in offspring of amphetamine treated rats. Previous observations have pointed out that treatment with amphetamine during pregnancy produces behavioral modifications in adulthood. In order to elucidate some possible brain biochemical mechanisms that could explain the behavioral changes obs... | [
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PMID:30096 | Role of neuroleptic agents on mouse pulmonary cyclic nucleotide systems. | The norepinephrine (NE)-induced accumulation of cyclic AMP in incubated tissue slices of mouse lung was inhibited by chlorpromazine (CPZ) and to a lesser extent by haloperidol. In particulate lung fractions both agents blocked dopamine-sensitive adenylate cyclase to a greater degree than the NE-responsive enzyme. Again... | Role of neuroleptic agents on mouse pulmonary cyclic nucleotide systems. The norepinephrine (NE)-induced accumulation of cyclic AMP in incubated tissue slices of mouse lung was inhibited by chlorpromazine (CPZ) and to a lesser extent by haloperidol. In particulate lung fractions both agents blocked dopamine-sensitive a... | [
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PMID:30098 | [Planning of psychotropic drug trials. Statistical evaluation. Hypotheses and methods]. | Any reasonable evaluation presupposes a precise question. Furthermore it is necessary to eliminate all factors of disturbance, which are able either to simulate or to mask a result of evaluation. The most important of these factors is the psychiatrist himself. Therefore, experimental designs have to taken in account th... | [Planning of psychotropic drug trials. Statistical evaluation. Hypotheses and methods]. Any reasonable evaluation presupposes a precise question. Furthermore it is necessary to eliminate all factors of disturbance, which are able either to simulate or to mask a result of evaluation. The most important of these factors ... | [
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PMID:30099 | Effect of alcohol and benzodiazepines on performance as related to personality characteristics. Personality characteristics among healthy "placebo reactors" and nonreactors. | For 2 weeks 40 volunteers received either 5 mg diazepam, t.i.d., or 10 mg chlordiazepoxide, t.i.d., and placebo. A choice reaction test, two coordination tests, and an attention test were administered to the subjects on the 14th day of each treatment. Thirty minutes before the tests, the subjects ingested either alcoho... | Effect of alcohol and benzodiazepines on performance as related to personality characteristics. Personality characteristics among healthy "placebo reactors" and nonreactors. For 2 weeks 40 volunteers received either 5 mg diazepam, t.i.d., or 10 mg chlordiazepoxide, t.i.d., and placebo. A choice reaction test, two coord... | [
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PMID:30100 | [Further developments of the AMP-system. A report on the 2nd AMP-symposium and training seminar (author's transl)]. | This is a report of a symposium and training seminar of the "Arbeitsgemeinschaft für Methodik und Dokumentation in der Psychiatrie" (AMP). Video-recordings of two psychiatric interviews were shown during the seminar and rated by the participants using the AMP-3-Scale (Psychopathology). Aim of the seminar was a rater tr... | [Further developments of the AMP-system. A report on the 2nd AMP-symposium and training seminar (author's transl)]. This is a report of a symposium and training seminar of the "Arbeitsgemeinschaft für Methodik und Dokumentation in der Psychiatrie" (AMP). Video-recordings of two psychiatric interviews were shown during ... | [
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PMID:30101 | Indication of an antipsychotic action of the opiate antagonist naloxone. | In 20 psychotic patients with frequent hallucinations and/or actual delusional experience a possible antipsychotic action of the opiate antagonist naloxone (N-allyl-noroxymorphone) was investigated, using a double-blind placebo-controlled cross-over design. 18 of these patients were not treated with neuroleptic drugs; ... | Indication of an antipsychotic action of the opiate antagonist naloxone. In 20 psychotic patients with frequent hallucinations and/or actual delusional experience a possible antipsychotic action of the opiate antagonist naloxone (N-allyl-noroxymorphone) was investigated, using a double-blind placebo-controlled cross-ov... | [
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PMID:30103 | [Influence of the Benzodiazepine-derivative Ro 06-9098/000 on choreo-athetotic syndromes (author's transl)]. | Five patients suffering from choreo-athetotic syndromes of different genesis were treated with the benzodiazepine-derivative Ro 06-9098/000 (7-Nitro-1(methylmethoxy)-1,3-dihydro-5-phenyl-2 H-1,4-benzodiazepin-2-one). The existing hyperkinesias could be well influenced in four cases of male patients, sufficiently in one... | [Influence of the Benzodiazepine-derivative Ro 06-9098/000 on choreo-athetotic syndromes (author's transl)]. Five patients suffering from choreo-athetotic syndromes of different genesis were treated with the benzodiazepine-derivative Ro 06-9098/000 (7-Nitro-1(methylmethoxy)-1,3-dihydro-5-phenyl-2 H-1,4-benzodiazepin-2-... | [
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PMID:30105 | [The preprogramming of behavior patterns by changing the neurotransmitter metabolism in the early postnatal ontogenesis of albino rats]. | Changes in neurotransmitter concentrations, which are brought about by the administration of neurotropic pharmaceuticals to albino rats at the time of differentiation of the brain, may have a lasting effect upon the reactivity and adaptability of adult animals to environmental factors and give rise to specific patterns... | [The preprogramming of behavior patterns by changing the neurotransmitter metabolism in the early postnatal ontogenesis of albino rats]. Changes in neurotransmitter concentrations, which are brought about by the administration of neurotropic pharmaceuticals to albino rats at the time of differentiation of the brain, ma... | [
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PMID:30106 | The striatonigral fibres and the feedback control of dopamine metabolism. | It proved possible to make lesions which interrupted the striatonigral GABA-containing pathway in the rat brain without causing concomitant damage to the nigrostriatal dopamine containing system. Estimations of striatal concentrations of dopamine (DA), dihydroxyphenyl-acetic acid (DOPAC) and homovanillic acid (HVA) ind... | The striatonigral fibres and the feedback control of dopamine metabolism. It proved possible to make lesions which interrupted the striatonigral GABA-containing pathway in the rat brain without causing concomitant damage to the nigrostriatal dopamine containing system. Estimations of striatal concentrations of dopamine... | [
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PMID:30110 | Benzodiazepines and behavioral effects of reward (water) omission in the rat. | Two behaviors related to nonreward (omission of water in an enclosure where the rats were habituated to drink) were studied. The time spent licking the bottles during water omission and the time spent drinking during a subsequent 5-min drinking session (water available) were recorded. The drinking session was performed... | Benzodiazepines and behavioral effects of reward (water) omission in the rat. Two behaviors related to nonreward (omission of water in an enclosure where the rats were habituated to drink) were studied. The time spent licking the bottles during water omission and the time spent drinking during a subsequent 5-min drinki... | [
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PMID:30115 | Pulmonary complications associated with the prune-belly syndrome. | Eight patients are presented who demonstrate many of the pulmonary complications seen in the prune-belly syndrome. The patients are divided into two major groups: Group I includes pulmonary hypoplasia; Group II includes lobar atelectasis and pneumonia. The etiology, pathogenesis, and radiographic features of these comp... | Pulmonary complications associated with the prune-belly syndrome. Eight patients are presented who demonstrate many of the pulmonary complications seen in the prune-belly syndrome. The patients are divided into two major groups: Group I includes pulmonary hypoplasia; Group II includes lobar atelectasis and pneumonia. T... | [
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PMID:30119 | Activation of guanylate cyclase by arachidonic acid in mammary gland homogenates from mice. | Arachidonic acid stimulated guanylate cyclase activity about two fold in homogenates of mammary glands obtained from midpregnant mice; effects of arachidonic acid were observed during incubation periods between 5 and 20 minutes. Stimulatory effects of arachidonic acid on guanylate cyclase activity were observed when 10... | Activation of guanylate cyclase by arachidonic acid in mammary gland homogenates from mice. Arachidonic acid stimulated guanylate cyclase activity about two fold in homogenates of mammary glands obtained from midpregnant mice; effects of arachidonic acid were observed during incubation periods between 5 and 20 minutes.... | [
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PMID:30122 | Effect of beta-fluorophenethylamine analogs on monoamine oxidase substrate preference. | A novel potentiometric assay was employed to evaluate the effects of beta-fluorination of phenethylamine (PEA) on brain MAO substrate preference. Through the use of the substrate selective MAO inhibitors clorgyline (type A) and Deprenyl (type B), F-PEA and F2-PEA were found to be preferred substrates for MAO type B. Si... | Effect of beta-fluorophenethylamine analogs on monoamine oxidase substrate preference. A novel potentiometric assay was employed to evaluate the effects of beta-fluorination of phenethylamine (PEA) on brain MAO substrate preference. Through the use of the substrate selective MAO inhibitors clorgyline (type A) and Depre... | [
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PMID:30123 | Survival of Leptospira interrogans serovar pomona in an acidic soil under simulated New Zealand field conditions. | Leptospira interrogans serovar pomona was found to survive for at least 42 days in a typical New Zealand soil under simulated winter field conditions. The soil was markedly acidic with a pH of 5.5 and survival times were not reduced even when its water content was only 23%. The values of both these parameters are consi... | Survival of Leptospira interrogans serovar pomona in an acidic soil under simulated New Zealand field conditions. Leptospira interrogans serovar pomona was found to survive for at least 42 days in a typical New Zealand soil under simulated winter field conditions. The soil was markedly acidic with a pH of 5.5 and survi... | [
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PMID:30124 | Tissue and blood distribution of gamma-glutamyl transferase in the lamb and in the ewe. | Gamma-glutamyl transferase distribution was studied in 10 ewes and 10 lambs; in both groups kidney was the most active organ, followed by pancreas, liver and lungs. In kidney and liver, GGT was bound mainly to cellular structures. Blood GGT activity of 48 lambs was 44 +/- 11 iu/litre and 33 +/- 7 iu/litre of 45 ewes. | Tissue and blood distribution of gamma-glutamyl transferase in the lamb and in the ewe. Gamma-glutamyl transferase distribution was studied in 10 ewes and 10 lambs; in both groups kidney was the most active organ, followed by pancreas, liver and lungs. In kidney and liver, GGT was bound mainly to cellular structures. B... | [
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PMID:30125 | Subcellular fractionation studies on peroral jejunal biopsies from the dog. | Portions of closed jejunal biopsies from the dog were homogenised and their organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal organelles were determined using highly sensitive assay procedures. The following organelles, with a... | Subcellular fractionation studies on peroral jejunal biopsies from the dog. Portions of closed jejunal biopsies from the dog were homogenised and their organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal organelles were determin... | [
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PMID:30128 | Gill ventilation in the sturgeon, Acipenser transmontanus: unusual adaptations for bottom dwelling. | Measurements of branchial cavity water pressures and flow patterns, arterial blood PO2 and pH, and oxygen utilization and uptake have been made in undisturbed, free swimming sturgeon, Acipenser transmontanus. Although the jaws are degenerate and the oral apparatus is highly modified for feeding, gill ventilation is non... | Gill ventilation in the sturgeon, Acipenser transmontanus: unusual adaptations for bottom dwelling. Measurements of branchial cavity water pressures and flow patterns, arterial blood PO2 and pH, and oxygen utilization and uptake have been made in undisturbed, free swimming sturgeon, Acipenser transmontanus. Although th... | [
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PMID:30129 | Hemoglobin-ligand interaction in fetal and maternal sheep blood. | Hemoglobin-ligand interaction was studied in maternal and fetal sheep blood as a function of oxygen saturation. pH was changed by varying CO2 concentration (CO2 Bohr effect) or by addition of NaOH or HCl at constant PCO2 (fixed acid Bohr effect). For maternal blood, CO2 Bohr factor was -0.41 at 50% oxygen saturation, i... | Hemoglobin-ligand interaction in fetal and maternal sheep blood. Hemoglobin-ligand interaction was studied in maternal and fetal sheep blood as a function of oxygen saturation. pH was changed by varying CO2 concentration (CO2 Bohr effect) or by addition of NaOH or HCl at constant PCO2 (fixed acid Bohr effect). For mate... | [
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PMID:30130 | Pulmonary gas exchange, diffusing capacity in natives and newcomers at high altitude. | At high altitude, in resting conditions, no differences have been observed between High Altitude Natives (HAN) and acclimatized Sea Level Natives (SLN) in AaDO2, aADCO2 or venous admixture. In acclimatized SLN, AaDO2 is smaller than at sea level because of: (1) The minor effect on arterial oxygenation of the probably c... | Pulmonary gas exchange, diffusing capacity in natives and newcomers at high altitude. At high altitude, in resting conditions, no differences have been observed between High Altitude Natives (HAN) and acclimatized Sea Level Natives (SLN) in AaDO2, aADCO2 or venous admixture. In acclimatized SLN, AaDO2 is smaller than a... | [
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PMID:30131 | Oxygen affinity in the blood of sheep. | The oxygen affinity in the blood of adult Dorset sheep with HbB was studied by determining several oxygen equilibrium curves under different conditions of pH and PCO2 by a method that allowed strict control of pH, PCO2, and HCO3- concentration over the entire curve. Nomograms and equations were derived that allowed the... | Oxygen affinity in the blood of sheep. The oxygen affinity in the blood of adult Dorset sheep with HbB was studied by determining several oxygen equilibrium curves under different conditions of pH and PCO2 by a method that allowed strict control of pH, PCO2, and HCO3- concentration over the entire curve. Nomograms and ... | [
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PMID:30163 | Separator isoelectric focusing for identification of alpha-1-antitrypsin (Pi M) subtypes. | A modified analytical isoelectric focusing procedure on polyacrylamide gel slabs, offering increased resolving capacity, has been developed for identification of six subtypes of the most common alpha-1-antitrypsin (A-1-AT) phenotype, Pi M. Combination of commercial Ampholine and other ampoteric substances, so called se... | Separator isoelectric focusing for identification of alpha-1-antitrypsin (Pi M) subtypes. A modified analytical isoelectric focusing procedure on polyacrylamide gel slabs, offering increased resolving capacity, has been developed for identification of six subtypes of the most common alpha-1-antitrypsin (A-1-AT) phenoty... | [
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PMID:30164 | Purification and properties of human amniotic fluid diamine oxidase. | Diamine oxidase (DAO) was purified from amniotic fluid. The activity was separated in two DAO fractions with pI values of 5.8 and 4.0. Molecular weight were found to be 245,000 and 485,000, respectively, with subunit molecular weight of 110,000. This indicated that they probably are dimer and tetramer of the same DAO s... | Purification and properties of human amniotic fluid diamine oxidase. Diamine oxidase (DAO) was purified from amniotic fluid. The activity was separated in two DAO fractions with pI values of 5.8 and 4.0. Molecular weight were found to be 245,000 and 485,000, respectively, with subunit molecular weight of 110,000. This ... | [
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PMID:30165 | Determination of plasma or serum haemoglobin by peroxidase activity employing 2,2'-azino-di-(3-ethyl-benzthiazolinsulphonate-6) as chromogen. | A procedure is described for the determination of plasma or serum haemoglobin employing the peroxidase activity of the haemoprotein using 2,2'-azino-di-(3-ethyl-benzthiazolinsulphonate-6) as chromogen. The method gives equal results for free haemoglobin, methaemoglobin and haemoglobin complexed to haptoglobin. It is de... | Determination of plasma or serum haemoglobin by peroxidase activity employing 2,2'-azino-di-(3-ethyl-benzthiazolinsulphonate-6) as chromogen. A procedure is described for the determination of plasma or serum haemoglobin employing the peroxidase activity of the haemoprotein using 2,2'-azino-di-(3-ethyl-benzthiazolinsulp... | [
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PMID:30173 | Viruses and bacteria associated with acute respiratory illnesses in young children in general practice. | The results obtained and the laboratory methods used for the isolation of viruses and bacteria from Malaysian children with acute respiratory illnesses seen in a private clinic are described. Of the 65 children studied virus isolations were obtained from 26 children, bacteria from 10 and both virus and bacteria from an... | Viruses and bacteria associated with acute respiratory illnesses in young children in general practice. The results obtained and the laboratory methods used for the isolation of viruses and bacteria from Malaysian children with acute respiratory illnesses seen in a private clinic are described. Of the 65 children studi... | [
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PMID:30178 | Current concepts of the undescended testis. | Abnormalities of testicular descent represent the most common genitourinary anomaly in men. Many aspects of testicular maldescent remain controversial. The etiology of the undescended testis is still not known, but results of experimental work in rats strongly support the hormonal theories. Distinction between the trul... | Current concepts of the undescended testis. Abnormalities of testicular descent represent the most common genitourinary anomaly in men. Many aspects of testicular maldescent remain controversial. The etiology of the undescended testis is still not known, but results of experimental work in rats strongly support the hor... | [
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PMID:30183 | Takayasu's disease: association with HLA-B5. | The frequency of HLA-B5 (66%, n = 38) in Japanese patients with Takayasu's disease was significantly higher than in controls (32%, n = 160). The level of significance was x2 = 13.53, P less than 0.0005 and corrected P less than 0.009. | Takayasu's disease: association with HLA-B5. The frequency of HLA-B5 (66%, n = 38) in Japanese patients with Takayasu's disease was significantly higher than in controls (32%, n = 160). The level of significance was x2 = 13.53, P less than 0.0005 and corrected P less than 0.009. | [
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PMID:30190 | The lethal effects of the cibarial and pharyngeal armatures of mosquitoes on microfilariae. | Microfilariae of Wuchereria bancrofti and Brugia pahangi were killed by the chewing action of the cibarial and pharyngeal armatures and other papillae and spines in the fore-gut of mosquitoes. The proportion of ingested microfilariae that were killed was largely dependent on the presence and shape of the cibarial armat... | The lethal effects of the cibarial and pharyngeal armatures of mosquitoes on microfilariae. Microfilariae of Wuchereria bancrofti and Brugia pahangi were killed by the chewing action of the cibarial and pharyngeal armatures and other papillae and spines in the fore-gut of mosquitoes. The proportion of ingested microfil... | [
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PMID:30192 | Mouse Peyer's patches contain T cells capable of inducing the graft-versus-host reaction (GVHR). | The potential of cells from the Peyer's patches (PP) of normal adult DBA/2 Tru mice (DBA/2) to induce a graft-versus-host reaction when injected into (C57BL/6 Tru x DBA/2 Tru)F1 hybrid (B6D2F1) mice was studied. The injection of 10(6) to 10(7) DBA/2 PP or spleen cells i.p. into neonatal F1 mice produced a striking spl... | Mouse Peyer's patches contain T cells capable of inducing the graft-versus-host reaction (GVHR). The potential of cells from the Peyer's patches (PP) of normal adult DBA/2 Tru mice (DBA/2) to induce a graft-versus-host reaction when injected into (C57BL/6 Tru x DBA/2 Tru)F1 hybrid (B6D2F1) mice was studied. The inject... | [
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PMID:30194 | ABO-incompatible marrow transplants. | Seventeen patients with aplastic anemia or acute leukemia received transplants from donors who had major ABO incompatibilities. Antibody titers were decreased by plasma and whole blood exchanges prior to marrow infusion. All 17 patients were successfully engrafted, and there was one possible rejection in the patient wi... | ABO-incompatible marrow transplants. Seventeen patients with aplastic anemia or acute leukemia received transplants from donors who had major ABO incompatibilities. Antibody titers were decreased by plasma and whole blood exchanges prior to marrow infusion. All 17 patients were successfully engrafted, and there was one... | [
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PMID:30195 | Lipoprotein-lipase activity in subcutaneous, adipose tissue in healthy subjects: variation of activity in a population of 60-year-old men. | The lipoprotein-lipase activity (LPLA) in the abdominal, subcutaneous, adipose tissue was studied in a random sample (n = 69) of 60-year-old men. A new method for the quantification of LPLA was applied. The mean value was 67 mU/g when expressed per gram (wet weight) of adipose tissue. Several subjects within the lower ... | Lipoprotein-lipase activity in subcutaneous, adipose tissue in healthy subjects: variation of activity in a population of 60-year-old men. The lipoprotein-lipase activity (LPLA) in the abdominal, subcutaneous, adipose tissue was studied in a random sample (n = 69) of 60-year-old men. A new method for the quantification... | [
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PMID:30196 | Postpubertal untreated cryptorchidism. Anatomical relationship between Leydig cells and tubular sclerosis. | A histological study of the postpubertal untreated cryptorchid testis establishes a direct anatomical relationship between Leydig cells and tubular sclerosis, an age-related process ranging from mild fibrosis of the tubular tunica propria to complete tubular hyalinization. Two separate processes are involved. Mild or m... | Postpubertal untreated cryptorchidism. Anatomical relationship between Leydig cells and tubular sclerosis. A histological study of the postpubertal untreated cryptorchid testis establishes a direct anatomical relationship between Leydig cells and tubular sclerosis, an age-related process ranging from mild fibrosis of t... | [
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PMID:30191 | Reversal of the storage lesion of CPD bank blood: a problem in clinical medicine. | The effect of phosphate buffer on the course of pH, ATP, and 2,3-PDG of CPD red blood cells stored at three temperatures was observed. Basic phosphate at an equilibrated level of 10 mM (as iP) maintained pH above 7.00 and ATP and 2,3-DPG above 70 per cent of initial value in cells stored at 37 C for 24 hours. In contra... | Reversal of the storage lesion of CPD bank blood: a problem in clinical medicine. The effect of phosphate buffer on the course of pH, ATP, and 2,3-PDG of CPD red blood cells stored at three temperatures was observed. Basic phosphate at an equilibrated level of 10 mM (as iP) maintained pH above 7.00 and ATP and 2,3-DPG ... | [
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PMID:30197 | Alpha-blockers and urethral pressure in neurological patients. | In 11 patients with upper motor neuron bladder paresis due to spinal trauma, 2 patients with lower motor neuron bladder paresis, and 4 patients with multiple sclerosis, alpha-adrenergic blockade with 10 mg Regitine intravenously decreased the maximum urethral closure pressure to an average of 42%. The decrease was most... | Alpha-blockers and urethral pressure in neurological patients. In 11 patients with upper motor neuron bladder paresis due to spinal trauma, 2 patients with lower motor neuron bladder paresis, and 4 patients with multiple sclerosis, alpha-adrenergic blockade with 10 mg Regitine intravenously decreased the maximum urethr... | [
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PMID:30198 | Detrusor hyperreflexia in female urinary incontinence treated pharmacologically. | Detrusor hyperreflexia was found in 54 patients or 14.6% of 369 consecutive patients referred for urinary incontinence and/or genital prolapse during a 2-year period. The dominant symptom was urge incontinence. The urological investigation consisted of a medium fill water cystometry in the supine position. 20 patients ... | Detrusor hyperreflexia in female urinary incontinence treated pharmacologically. Detrusor hyperreflexia was found in 54 patients or 14.6% of 369 consecutive patients referred for urinary incontinence and/or genital prolapse during a 2-year period. The dominant symptom was urge incontinence. The urological investigation... | [
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PMID:30199 | Effect of beta-adrenoceptor stimulation on the human bladder in vivo. | The effect of beta-adrenoceptor stimulation on the volume of the urinary bladder in 16 neurologically normal humans without symptoms of micturition disturbances was investigated into. Terbutaline, a selective beta2-receptor-stimulating agent, was tested in 9 persons and isoprenaline, a general beta-receptor-stimulating... | Effect of beta-adrenoceptor stimulation on the human bladder in vivo. The effect of beta-adrenoceptor stimulation on the volume of the urinary bladder in 16 neurologically normal humans without symptoms of micturition disturbances was investigated into. Terbutaline, a selective beta2-receptor-stimulating agent, was tes... | [
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PMID:30200 | Effects of beta-adrenergic stimulating and blocking agents on the dynamics of the human bladder outlet. | The alpha-adrenergic innervation of the functional urethra is a well-known fact, while beta-adrenergic influence is rather unknown until now. We studied the influence of beta-stimulating and beta-blocking agents on the human urethra by the urethral pressure profile (UPP). A decrease of the UPP under orciprenaline sulfa... | Effects of beta-adrenergic stimulating and blocking agents on the dynamics of the human bladder outlet. The alpha-adrenergic innervation of the functional urethra is a well-known fact, while beta-adrenergic influence is rather unknown until now. We studied the influence of beta-stimulating and beta-blocking agents on t... | [
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PMID:30202 | Twenty-five-year experience with prune belly syndrome. | Thirty cases of prune belly syndrome with no stillborn deaths seen over a twenty-five-year period at a university pediatric hospital are reviewed and the management, findings, and prognosis discussed. | Twenty-five-year experience with prune belly syndrome. Thirty cases of prune belly syndrome with no stillborn deaths seen over a twenty-five-year period at a university pediatric hospital are reviewed and the management, findings, and prognosis discussed. | [
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PMID:30203 | Urinary tract reconstruction in prune belly syndrome. | Fifteen boys with prune belly syndrome cared for over a ten-year period required surgical treatment because of uncontrolled infection and/or progressive azotemia. Five died of renal dysplasia. Primary or staged reconstruction resulted in significant improvement when ureteral reimplantation was combined with tailoring o... | Urinary tract reconstruction in prune belly syndrome. Fifteen boys with prune belly syndrome cared for over a ten-year period required surgical treatment because of uncontrolled infection and/or progressive azotemia. Five died of renal dysplasia. Primary or staged reconstruction resulted in significant improvement when... | [
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PMID:30216 | [Age-dependent adaptation of erythrocytes in the model of haemoglobinolysis (author's transl)]. | 1. The erythrocyte is suitable as a model of investigating the mechanism of adaptation as a function of aging. 2. By hemolysis experiments in isotonic solutions of various pH-values changes of the resistance of human erythrocytes during the mechanism of aging in the organism are established. 3. In isotonic alkaline so... | [Age-dependent adaptation of erythrocytes in the model of haemoglobinolysis (author's transl)]. 1. The erythrocyte is suitable as a model of investigating the mechanism of adaptation as a function of aging. 2. By hemolysis experiments in isotonic solutions of various pH-values changes of the resistance of human erythr... | [
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PMID:30211 | [Effect of low temperatures on the functional state of the membranes of the hepatic endoplasmic reticulum]. | Oxidation of fluorescent substrates--NADPH and 4-dimethylamine chalcone (DMC) by microsomes from liver endoplasmic reticulum was studied after freezing and thawing of these cells organelles. Slow freezing to -25 degrees or quick freezing to -196 degrees did not affect distinctly the rate of NADPH oxidation after thawin... | [Effect of low temperatures on the functional state of the membranes of the hepatic endoplasmic reticulum]. Oxidation of fluorescent substrates--NADPH and 4-dimethylamine chalcone (DMC) by microsomes from liver endoplasmic reticulum was studied after freezing and thawing of these cells organelles. Slow freezing to -25 ... | [
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PMID:30212 | [Concentration of cyclic nucleotides, activity of adenylate cyclase, 3',5'-AMP phosphodiesterase and guanylate cyclase in plasma membranes from liver and hepatomas of different degrees of malignancy]. | Adenylate cyclase activity as well as intracellular content of sAMP were decreased 2.5-4-fold, as compared with normal state, in plasmatic membranes (PM) of hepatoma 22 and of Ehrlich ascites carcinoma--the tumors characterized by high level- of malignancy. Activity of cAMP phosphodiesterase exceeded distinctly the nor... | [Concentration of cyclic nucleotides, activity of adenylate cyclase, 3',5'-AMP phosphodiesterase and guanylate cyclase in plasma membranes from liver and hepatomas of different degrees of malignancy]. Adenylate cyclase activity as well as intracellular content of sAMP were decreased 2.5-4-fold, as compared with normal ... | [
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PMID:30219 | [Lipids and lipoproteins in early changes of arteriosclerosis]. | LDL, fibrinogen and other plasma macromolecules are retained in intima in substantial amounts. In areas where lipid is accumulating a large part of LDL is immobilized possibily by binding to fibrin. Lipid may be deposited from LDL as a result of degradation by a cathepsin in intima. | [Lipids and lipoproteins in early changes of arteriosclerosis]. LDL, fibrinogen and other plasma macromolecules are retained in intima in substantial amounts. In areas where lipid is accumulating a large part of LDL is immobilized possibily by binding to fibrin. Lipid may be deposited from LDL as a result of degradatio... | [
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PMID:30220 | [The coincidence of visceral lupus erythematosus and epilepsy]. | It is reported on course and problems of the diagnosis of a lupus erythematodes visceralis with epilepsy. In the demonstrated case the initial stage was characterized by the nearly simultaneous appearance of relapsing arthritides and epilepsy. The diagnosis was made in the stage of an acute exacerbation with cardiac sy... | [The coincidence of visceral lupus erythematosus and epilepsy]. It is reported on course and problems of the diagnosis of a lupus erythematodes visceralis with epilepsy. In the demonstrated case the initial stage was characterized by the nearly simultaneous appearance of relapsing arthritides and epilepsy. The diagnosi... | [
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PMID:30228 | [GLC-data of 19 hydrolysis-derivatives rised from 12 important benzodiazepines and 17 main-metabolites (author's transl)]. | Analytical investigations of extracts after acid hydrolysis are in use especially considering benzodiazepine screening methods. The article describes the gas-chromatographic data of 19 hydrolysis-derivatives which are formed by hydrolysis of 12 important 1,4- and 1,5-benzodiazepines and 17 main metabolites. | [GLC-data of 19 hydrolysis-derivatives rised from 12 important benzodiazepines and 17 main-metabolites (author's transl)]. Analytical investigations of extracts after acid hydrolysis are in use especially considering benzodiazepine screening methods. The article describes the gas-chromatographic data of 19 hydrolysis-d... | [
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PMID:30245 | [Use of phenazepam in the treatment of neurotic conditions]. | The report contains data concerning clinical studies of a new Soviet tranquilizer of benzodiazepine derivative--phenazepam. Treatment of 100 patients gave a good therapeutical effect in 82%. The best results were attained in the treatment of generalized hystero-neurotical states, somewhat less in the group of neurotic ... | [Use of phenazepam in the treatment of neurotic conditions]. The report contains data concerning clinical studies of a new Soviet tranquilizer of benzodiazepine derivative--phenazepam. Treatment of 100 patients gave a good therapeutical effect in 82%. The best results were attained in the treatment of generalized hyste... | [
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PMID:30246 | Cytochrome P-450 and alkane hydroxylase activity in Candida guilliermondii. | In the investigated Candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nMol cytochrome P-450 per g cells (wet weight) could be detected. Cytochrome P-450 and alkane hydroxylase activity was found in the 100 000 xg pellet. Cofactor studies and inhibition experiments reveal... | Cytochrome P-450 and alkane hydroxylase activity in Candida guilliermondii. In the investigated Candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nMol cytochrome P-450 per g cells (wet weight) could be detected. Cytochrome P-450 and alkane hydroxylase activity was found ... | [
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PMID:30247 | Methotrexate and citrovorum factor after histoincompatible allogeneic bone marrow transplants in dogs. | Methotrexate (MTX) followed by citrovorum factor (CVF) rescue was evaluated for its effectiveness in reducing graft-versus-host disease (GVHD) in lethally irradiated dogs transplanted with bone marrow from unrelated histoincompatible donors. Animals were given no immunosuppressive therapy (group A) or a combined regime... | Methotrexate and citrovorum factor after histoincompatible allogeneic bone marrow transplants in dogs. Methotrexate (MTX) followed by citrovorum factor (CVF) rescue was evaluated for its effectiveness in reducing graft-versus-host disease (GVHD) in lethally irradiated dogs transplanted with bone marrow from unrelated h... | [
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PMID:30248 | Comparison between the unsaturated plasma folate binder and in vivo labelled plasma folate binder. | Endogenous plasma folate binder denuded of folate by dialysis at pH 3, subsequently bound more methylfolate than folic acid, in contrast with the minor unsaturated plasma binder which bound folic acid in preference to methylfolate. On Sephadex DEAE-A50 chromatography 14C-CH3H4PteGlu bound to acid-denuded endogenous bin... | Comparison between the unsaturated plasma folate binder and in vivo labelled plasma folate binder. Endogenous plasma folate binder denuded of folate by dialysis at pH 3, subsequently bound more methylfolate than folic acid, in contrast with the minor unsaturated plasma binder which bound folic acid in preference to met... | [
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PMID:30249 | Peripheral platelet count in response to salbutamol before and after adrenergic beta-receptor blockade. | The effect of salbutamol (a comparatively selective adrenergic beta2-receptor agonist) on the peripheral platelet concentration was studied before and after the ingestion of either 50 mg metoprolol or 40 mg propranolol. The study was carried out on healthy males volunteers and autologous 51Cr-labelled platelets were em... | Peripheral platelet count in response to salbutamol before and after adrenergic beta-receptor blockade. The effect of salbutamol (a comparatively selective adrenergic beta2-receptor agonist) on the peripheral platelet concentration was studied before and after the ingestion of either 50 mg metoprolol or 40 mg propranol... | [
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PMID:30250 | Clinical restitution following cerebral ischemia in hypo-, normo- and hyperglycemic rats. | Rats with different levels of blood glucose concentration were exposed to 10 min of complete brain ischemia achieved by compression of neck vessels by a pneumatic cuff. All normoglycemic rats survived the ischemic period and made the best clinical recovery. Hyperglycemic rats died within 12 h. Seizure activity was obse... | Clinical restitution following cerebral ischemia in hypo-, normo- and hyperglycemic rats. Rats with different levels of blood glucose concentration were exposed to 10 min of complete brain ischemia achieved by compression of neck vessels by a pneumatic cuff. All normoglycemic rats survived the ischemic period and made ... | [
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PMID:30251 | beta-Glucosidase activities in the Norrbotten type of juvenile Gaucher's disease. | beta-Glucosidase and N-acetyl-beta-glucosaminidase activities were measured with synthetic substrates in peripheral leucocytes, urine and serum from patients with juvenile type of Gaucher's disease. Our findings in urine and serum make it clear that diagnosis by using synthetic substrate is not possible. In peripheral ... | beta-Glucosidase activities in the Norrbotten type of juvenile Gaucher's disease. beta-Glucosidase and N-acetyl-beta-glucosaminidase activities were measured with synthetic substrates in peripheral leucocytes, urine and serum from patients with juvenile type of Gaucher's disease. Our findings in urine and serum make it... | [
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PMID:30252 | The effect of stannous fluoride on human plaque acidogenicity in situ (Stephan curve). | A system employing an Ingold glass electrode was shown to give reliable measurements of pH drops in dental plaque in situ (Stephan curve readings). The system was used to demonstrate that mouthrinses of 0.2 per cent aqueous solutions of stannous fluoride reduced the pH drops markedly for at least seven hours. A reducti... | The effect of stannous fluoride on human plaque acidogenicity in situ (Stephan curve). A system employing an Ingold glass electrode was shown to give reliable measurements of pH drops in dental plaque in situ (Stephan curve readings). The system was used to demonstrate that mouthrinses of 0.2 per cent aqueous solutions... | [
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PMID:30254 | Observations of otoconial membranes from human infants. | The microdissection technique was used to study otoconial membrane from 30 human infants ranging from newborn to 2 years of age. Both saccular and utricular membranes were quite variable in overall shape. During the neonatal period, the gelatinous layer of the otoconial membrane appears to thicken and become less adher... | Observations of otoconial membranes from human infants. The microdissection technique was used to study otoconial membrane from 30 human infants ranging from newborn to 2 years of age. Both saccular and utricular membranes were quite variable in overall shape. During the neonatal period, the gelatinous layer of the oto... | [
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PMID:30255 | Bacteriology of maxillary sinusitis in relation to quality of the retained secretion. | The bacteriological findings in 200 patients with acute maxillary sinusitis are reported. It is concluded that the sampling technique--by antral aspiration--is highly significant in the evaluation of the bacteriological background of sinusitis, whereas the anerobic transport of the sample seems to be of less importance... | Bacteriology of maxillary sinusitis in relation to quality of the retained secretion. The bacteriological findings in 200 patients with acute maxillary sinusitis are reported. It is concluded that the sampling technique--by antral aspiration--is highly significant in the evaluation of the bacteriological background of ... | [
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PMID:30261 | Base pairing mRNA-rRNA. The arrangement of nucleotides in the message for coat protein of phage MS 2. | The relation of the nucleotide sequences in the message and of the short nucleotide sequence in 3' terminus of 16 S ribosomal RNA was found to differ from statistical expectancy. The observation is interpreted in terms of both the ribosomal interactions and the molecular evolution. | Base pairing mRNA-rRNA. The arrangement of nucleotides in the message for coat protein of phage MS 2. The relation of the nucleotide sequences in the message and of the short nucleotide sequence in 3' terminus of 16 S ribosomal RNA was found to differ from statistical expectancy. The observation is interpreted in terms... | [
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PMID:30262 | Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. I. Gel filtration in 6 M guanidine hydrochloride. | Highly purified glycopolypeptides HA1 and HA2 were separated from bromelain-released haemagglutinin of influenza virus A/Dunedin/4/73 (H3N2) by gel filtration in 6 M guanidine hydrochloride under reducing conditions. The purity of both glycopolypeptides was proved by extensive studies. Despite the lack of C-terminal en... | Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. I. Gel filtration in 6 M guanidine hydrochloride. Highly purified glycopolypeptides HA1 and HA2 were separated from bromelain-released haemagglutinin of influenza virus A/Dunedin/4/73 (H3N2) by gel filtration in 6 M guanidine hydrochloride under... | [
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PMID:30263 | UV-inactivation of Epstein-Barr virus: differences in early antigen expression in two different non-productive cell lines and influence of caffeine. | Two non-productive Epstein-Barr (EB) virus genome-carrying lymphoblastoid cell lines, namely Raji and NC37, were used for studying the effect of UV irradiation on the ability of P3HR-1 EB virus to induce early antigen (EA) formation. In NC37 cells infected with UV-irradiated virus the formation of EA was delayed; thus ... | UV-inactivation of Epstein-Barr virus: differences in early antigen expression in two different non-productive cell lines and influence of caffeine. Two non-productive Epstein-Barr (EB) virus genome-carrying lymphoblastoid cell lines, namely Raji and NC37, were used for studying the effect of UV irradiation on the abil... | [
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PMID:30264 | Suppression of an arenavirus by a togavirus in experimental acute double infection. | Lymphocytic choriomeningitis virus (LCMV) and tick-borne encephalitis virus (TEV) were inoculated in young and old chick embryo cell (CEC) and in L-cell cultures at various multiplicities per cell, simultaneously or 17 hr apart. The yield of infective LCMV was inhibited in double infection, most in old CEC with elevate... | Suppression of an arenavirus by a togavirus in experimental acute double infection. Lymphocytic choriomeningitis virus (LCMV) and tick-borne encephalitis virus (TEV) were inoculated in young and old chick embryo cell (CEC) and in L-cell cultures at various multiplicities per cell, simultaneously or 17 hr apart. The yie... | [
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PMID:30265 | Variations in biological properties of geographic strains of western equine encephalomyelitis virus before and after passage in Culex tarsalis and Culiseta melanura. | Western equine encephalomyelitis (WEE) virus strains from the eastern and western United States were compared using two biological markers: antigenic properties measured by cross complement fixation (CF) tests and the size of plaques produced in Vero cell cultures. Slight antigenic differences were discernible on a geo... | Variations in biological properties of geographic strains of western equine encephalomyelitis virus before and after passage in Culex tarsalis and Culiseta melanura. Western equine encephalomyelitis (WEE) virus strains from the eastern and western United States were compared using two biological markers: antigenic prop... | [
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PMID:30266 | Improved detection by immunodiffusion of type-specific influenza antibody in avian sera. | Various solvents and kinds of agar and agarose as well as various ribonucleoprotein (RNP) antigen preparations were tested in a search for optimal conditions for the detection of low levels of type-specific influenza antibody in avian sera by gel double diffusion. The best results were obtained with one kind of agarose... | Improved detection by immunodiffusion of type-specific influenza antibody in avian sera. Various solvents and kinds of agar and agarose as well as various ribonucleoprotein (RNP) antigen preparations were tested in a search for optimal conditions for the detection of low levels of type-specific influenza antibody in av... | [
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PMID:30267 | Experimental infection of hare (Lepus europaeus) with Coxiella burnetii and Rickettsia slovaca. | Hares (Lepus europaeus L.) infected subcutaneously (s.c.) or per os with Coxiella burnetii and s.c. with Rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of rickettsiae to different organs and variable antibody response. Neither riskettsia could be detected in ... | Experimental infection of hare (Lepus europaeus) with Coxiella burnetii and Rickettsia slovaca. Hares (Lepus europaeus L.) infected subcutaneously (s.c.) or per os with Coxiella burnetii and s.c. with Rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of ricketts... | [
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PMID:30268 | H-1 and X14 parvovirus antibodies in women with abortions or still-births. | Antibodies against H-1 and X14 parvoviruses were found 13.71 and 9.14%, respectively, of 350 sera from women affected by repeated abortions or still-births. On the contrary, only 2.66 and 1.66% of 300 control sera were positive to the two viruses. The difference in the incidence of antibodies appears highly significant... | H-1 and X14 parvovirus antibodies in women with abortions or still-births. Antibodies against H-1 and X14 parvoviruses were found 13.71 and 9.14%, respectively, of 350 sera from women affected by repeated abortions or still-births. On the contrary, only 2.66 and 1.66% of 300 control sera were positive to the two viruse... | [
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PMID:30271 | Depression: a new approach to an old syndrome. | Depression is a medical illness related to an interplay of genetic, biochemical and environmental/experiential factors. Careful diagnosis, using contemporary psychiatric criteria, simplifies treatment by the family physician. Awareness of these criteria may lead to improved selection of candidates for drug treatment an... | Depression: a new approach to an old syndrome. Depression is a medical illness related to an interplay of genetic, biochemical and environmental/experiential factors. Careful diagnosis, using contemporary psychiatric criteria, simplifies treatment by the family physician. Awareness of these criteria may lead to improve... | [
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PMID:30273 | Binding of bile acids by dietary fiber. | Binding of bile salts to food residue was studied in vitro and in vivo. In the in vitro experiments, residues of a number of foods were incubated with each of several bile salts at different concentrations and pHs. All food residues tested adsorbed more dihydroxy than trihydroxy bile salts. Bile salt binding increased ... | Binding of bile acids by dietary fiber. Binding of bile salts to food residue was studied in vitro and in vivo. In the in vitro experiments, residues of a number of foods were incubated with each of several bile salts at different concentrations and pHs. All food residues tested adsorbed more dihydroxy than trihydroxy ... | [
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PMID:30274 | Accuracy and precision of urinary pH determinations using two commercially available dipsticks. | The accuracy and precision of urinary pH determinations using commercially available dipsticks were studied. Two products were evaluated by 17 MT(ASCP) technologists over the pH range 4.5 to 9.0. The study included accuracy, reproducibility, variation among technologists, and variation between products. The results dem... | Accuracy and precision of urinary pH determinations using two commercially available dipsticks. The accuracy and precision of urinary pH determinations using commercially available dipsticks were studied. Two products were evaluated by 17 MT(ASCP) technologists over the pH range 4.5 to 9.0. The study included accuracy,... | [
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PMID:30276 | Urinary nitrite and urinary-tract infection. | Two dipstick procedures and an automated quantitative urinary nitrite assay were used to study nitrite in 786 samples of urine submitted to the bacteriology laboratory for routine culture and sensitivity testing. Many samples that had more than 100,000 nitrite-reducing organisms/ml and no detectable nitrite were studie... | Urinary nitrite and urinary-tract infection. Two dipstick procedures and an automated quantitative urinary nitrite assay were used to study nitrite in 786 samples of urine submitted to the bacteriology laboratory for routine culture and sensitivity testing. Many samples that had more than 100,000 nitrite-reducing organ... | [
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PMID:30277 | Inhibition of canine gastric acid secretion by an H-1 receptor antagonist to histamine. | Histamine H-2 receptors are thought to mediate gastric acid secretory responses, whereas H-1 receptors supposedly regulate mucosal vascular responses to histamine. In an in vivo chambered canine stomach flap preparation, the H-1 receptor antagonist, tripelennamine, injected intraarterially (22.1 mumol/kg) into the stom... | Inhibition of canine gastric acid secretion by an H-1 receptor antagonist to histamine. Histamine H-2 receptors are thought to mediate gastric acid secretory responses, whereas H-1 receptors supposedly regulate mucosal vascular responses to histamine. In an in vivo chambered canine stomach flap preparation, the H-1 rec... | [
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PMID:30279 | Impairment of secretin release in celiac sprue. | Plasma secretin concentrations and pH in the second portion of duodenum were measured in the fasting state and during duodenal infusion of HCl in five patients with untreated celiac sprue, five celiac sprue patients after gluten-free diets, and five normal subjects. Mean fasting plasma secretin concentrations were insi... | Impairment of secretin release in celiac sprue. Plasma secretin concentrations and pH in the second portion of duodenum were measured in the fasting state and during duodenal infusion of HCl in five patients with untreated celiac sprue, five celiac sprue patients after gluten-free diets, and five normal subjects. Mean ... | [
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PMID:30280 | Rapid emesis from high-dose ipecac syrup in adults and children intoxicated with antiemetics or other drugs. | The effect of ipecac syrup as an emetic in adults as well as children who had ingested antiemetics or other drugs was evaluated. Adults or children over five years of age were given 30 ml of ipecac syrup followed by 360 ml of water; children aged one to five years were given 15 ml ipecac syrup followed by 240 ml of wat... | Rapid emesis from high-dose ipecac syrup in adults and children intoxicated with antiemetics or other drugs. The effect of ipecac syrup as an emetic in adults as well as children who had ingested antiemetics or other drugs was evaluated. Adults or children over five years of age were given 30 ml of ipecac syrup followe... | [
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PMID:30282 | Cardiac systolic time intervals in fetal monkeys: pre-ejection period. | The systolic time intervals of the fetal cardiac cycle were studied by means of simultaneous recordings of electrocardiogram (ECG) and ultrasound Doppler cardiogram (DCG) in chronic preparations of fetal rhesus monkeys. Recordings were made under physiologic conditions as well as during various experimental stresses. T... | Cardiac systolic time intervals in fetal monkeys: pre-ejection period. The systolic time intervals of the fetal cardiac cycle were studied by means of simultaneous recordings of electrocardiogram (ECG) and ultrasound Doppler cardiogram (DCG) in chronic preparations of fetal rhesus monkeys. Recordings were made under ph... | [
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PMID:30287 | Withdrawal syndromes associated with antipsychotic drugs. | Withdrawal symptoms frequently follow abrupt discontinuation of antipsychotic compounds. In addition to other somatic symptoms, withdrawal-emergent dyskinesias may be observed. "Covert dyskinesia" refers to a masked form of tardive dyskinesia that becomes clinically detectable only after antipsychotic drugs are withdra... | Withdrawal syndromes associated with antipsychotic drugs. Withdrawal symptoms frequently follow abrupt discontinuation of antipsychotic compounds. In addition to other somatic symptoms, withdrawal-emergent dyskinesias may be observed. "Covert dyskinesia" refers to a masked form of tardive dyskinesia that becomes clinic... | [
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PMID:30288 | The social context of schizophrenia. | During the past 25 years psychiatry has increased its understanding of the social context of schizophrenia in four major areas. Reasonable reliability can now be achieved in describing and recognizing many of the acute and chronic syndromes, so that comparability can be achieved between different research teams. Much i... | The social context of schizophrenia. During the past 25 years psychiatry has increased its understanding of the social context of schizophrenia in four major areas. Reasonable reliability can now be achieved in describing and recognizing many of the acute and chronic syndromes, so that comparability can be achieved bet... | [
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PMID:30295 | [Medical check-ups and selection of subjects: some results from a gerontological field-study (author's transl)]. | In a gerontological field-study in Cologne, West-Germany, 1114 subjects of a random sample of elderlies were successively offered three modalities for a medical check-up, which covered a physical examination and a psychiatric interview: 1. in the out-patient department of a psychiatric hospital (Rheinische Landesklinik... | [Medical check-ups and selection of subjects: some results from a gerontological field-study (author's transl)]. In a gerontological field-study in Cologne, West-Germany, 1114 subjects of a random sample of elderlies were successively offered three modalities for a medical check-up, which covered a physical examination... | [
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PMID:30296 | [Collecting data on the state of health of the elderly by mail survey of family doctors (author's transl)]. | In connection with a gerontological research project data on the physical of the persons being investigated were, amongst other sources, also obtained through a mail survey of family doctors. How far survey shortcomings have a distorting effect was studied. The results indicate that a mail survey of family doctors may ... | [Collecting data on the state of health of the elderly by mail survey of family doctors (author's transl)]. In connection with a gerontological research project data on the physical of the persons being investigated were, amongst other sources, also obtained through a mail survey of family doctors. How far survey short... | [
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PMID:30297 | [Study on the effectiveness of the "Berlin Letters for Seniors (author's transl)]. | The effectiveness of the "Berlin Letters for Seniors" (LFS) will be tested by a longitudinal study. Two Measurement Points (MP) are scheduled: 1976 and 1978. The total sample (N = 3000) comprehends two subsamples of receivers and non-receivers. Data were/will be collected by a questionnaire. A preliminary analysis of t... | [Study on the effectiveness of the "Berlin Letters for Seniors (author's transl)]. The effectiveness of the "Berlin Letters for Seniors" (LFS) will be tested by a longitudinal study. Two Measurement Points (MP) are scheduled: 1976 and 1978. The total sample (N = 3000) comprehends two subsamples of receivers and non-rec... | [
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PMID:30298 | [Stress of the elderly (author's transl)]. | Author examines the suicide-motives of elderly people in connection with stress and describes the Hedri-Mallisons disease, which has a very high suicide-rate. | [Stress of the elderly (author's transl)]. Author examines the suicide-motives of elderly people in connection with stress and describes the Hedri-Mallisons disease, which has a very high suicide-rate. | [
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PMID:30324 | [Survived brain infarction in old age - clinical and morphological findings. II. Risk factors (author's transl)]. | 208 hospitalized patients, nearly 80 years old, were investigated because of risk factors and complicating diseases. Hypertension (58.2%), typical myocardial infarctions (37.2%) and diabetes (45.2%) were twice often as in our comparable cases without stroke. Corresponding we found signs of left ventricular hypertrophy ... | [Survived brain infarction in old age - clinical and morphological findings. II. Risk factors (author's transl)]. 208 hospitalized patients, nearly 80 years old, were investigated because of risk factors and complicating diseases. Hypertension (58.2%), typical myocardial infarctions (37.2%) and diabetes (45.2%) were tw... | [
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PMID:30325 | [Prosthetic fitting in the geriatric leg amputee (author's transl)]. | This retrospective study regarding prosthetic fitting in geriatric amputees showed that only about 50 per cent of the patients could be fitted with a prosthesis. The time interval from the amputation until the patients walked adequately with the prostheses was very long. This was due to problems with the healing of the... | [Prosthetic fitting in the geriatric leg amputee (author's transl)]. This retrospective study regarding prosthetic fitting in geriatric amputees showed that only about 50 per cent of the patients could be fitted with a prosthesis. The time interval from the amputation until the patients walked adequately with the prost... | [
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PMID:30326 | [Application of laser rays in surgery (author's transl)]. | Some years will have to pass, until there will be evidence, if application of leaser beam in surgery of Breastcancer, Melanomas or Basaliomas was justified and whether it is possible or not to interrupt or reduce intraoperative tumor cellspread. As an increasing number of surgeons have started to use laser rays in thes... | [Application of laser rays in surgery (author's transl)]. Some years will have to pass, until there will be evidence, if application of leaser beam in surgery of Breastcancer, Melanomas or Basaliomas was justified and whether it is possible or not to interrupt or reduce intraoperative tumor cellspread. As an increasing... | [
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PMID:30327 | [The cardiotropic effect of antidepressants in aging patients (author's transl)]. | All antidepressants now used have a cardiotropic, for some instances also cardiotoxic effect. This quinidine-like effect prolongs the QT-interval of ECG and can induce reentry and ventricular fibrillation. When quinidine and antidepressants are combined in the same patient the risk of such cardiac complications increas... | [The cardiotropic effect of antidepressants in aging patients (author's transl)]. All antidepressants now used have a cardiotropic, for some instances also cardiotoxic effect. This quinidine-like effect prolongs the QT-interval of ECG and can induce reentry and ventricular fibrillation. When quinidine and antidepressan... | [
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PMID:30328 | [Autoimmune phenomena in the aging, cerebral atherosclerosis, Parkinsonism (author's transl)]. | Studies were undertaken on the immune reactions in aging at cerebral atherosclerosis and parkinsonism. With age a number of changes occurred, which were characterized by a decrease in specific and non-specific immunity, against the background of which the autoimmune reactions were developing. There was an increase in t... | [Autoimmune phenomena in the aging, cerebral atherosclerosis, Parkinsonism (author's transl)]. Studies were undertaken on the immune reactions in aging at cerebral atherosclerosis and parkinsonism. With age a number of changes occurred, which were characterized by a decrease in specific and non-specific immunity, again... | [
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PMID:30329 | [A meaningful antibiotic therapy of diseases of the ear, nose and throat in old age (author's transl)]. | Each stage of life is characterized by the prevalence of certain affections; among others, inflammatory diseases of the ear, nose and throat are frequent in old age. Therefore, specific problems of chemotherapy in old age are dealt with in general terms in the introduction, with special stress being placed on the funct... | [A meaningful antibiotic therapy of diseases of the ear, nose and throat in old age (author's transl)]. Each stage of life is characterized by the prevalence of certain affections; among others, inflammatory diseases of the ear, nose and throat are frequent in old age. Therefore, specific problems of chemotherapy in ol... | [
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PMID:30330 | [Stereological investigation in the cerebral cortex of aging subjects (author's transl)]. | Involving cortical regions, capillaries of the human cerebrum of two 19 and 27 years old men, a 69 years old woman and a 72 years old man were stereologically investigated by optical-electronic image-analysis. The cortical capillary net work was demonstrated by the alkaline phosphatase activity. Each cortex region comp... | [Stereological investigation in the cerebral cortex of aging subjects (author's transl)]. Involving cortical regions, capillaries of the human cerebrum of two 19 and 27 years old men, a 69 years old woman and a 72 years old man were stereologically investigated by optical-electronic image-analysis. The cortical capilla... | [
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... |
PMID:30331 | Leucocyte migration test and hypersensitivity to glafenin. | The leucocyte migration test (LMT) was performed on 20 patients with an intolerance to glafenin--a non-narcotic analgesic drug. LMT was found to be positive in 50% of the subjects with intolerance, a highly significant percentage as compared with the control groups. HSA-glafenin was found to be the most appropriate met... | Leucocyte migration test and hypersensitivity to glafenin. The leucocyte migration test (LMT) was performed on 20 patients with an intolerance to glafenin--a non-narcotic analgesic drug. LMT was found to be positive in 50% of the subjects with intolerance, a highly significant percentage as compared with the control gr... | [
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0.024547213688492775,
0.010257718153297901,
0.014167357236146927,
0.04023... |
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