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PMID:5413 | pH dependence of the binding constants of N-acetylglucosamine monomers to hen and turkey egg-white lysozymes. | The binding constants of N-acetylglucosamine (G1cNAc) and its methyl alpha- and beta- glycosides to hen and turkey egg-white lysozymes [EC 3.2.1.17], in the latter of which Asp 101 is replaced by Gly, were determined at various pH values by measuring changes in the circular dichroic (DC) band at 295 nm. The binding of ... | pH dependence of the binding constants of N-acetylglucosamine monomers to hen and turkey egg-white lysozymes. The binding constants of N-acetylglucosamine (G1cNAc) and its methyl alpha- and beta- glycosides to hen and turkey egg-white lysozymes [EC 3.2.1.17], in the latter of which Asp 101 is replaced by Gly, were dete... | [
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PMID:5414 | Elementary processes in the interaction of serine protease with a possible transition state analog. Subtillisin-benzeneboronic acid system. | The interaction of benzeneboronic acid(BBA), a possible transition state analog, with subtilisin BPN' [EC 3.4.21.14] was studied by the temperature-jump method at various pH's, temperatures and in D2O as well as H2O. From analysis of the concentration dependence of the relaxation times, it was suggested that the subtil... | Elementary processes in the interaction of serine protease with a possible transition state analog. Subtillisin-benzeneboronic acid system. The interaction of benzeneboronic acid(BBA), a possible transition state analog, with subtilisin BPN' [EC 3.4.21.14] was studied by the temperature-jump method at various pH's, tem... | [
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PMID:5415 | Kinetic studies of carboxypeptidase Y. III. Action on ester, amide, and anilide substrates and the effects of some environmental factors. | Kinetic parameters of carboxypeptidase Y are given for the hydrolyses of ester, amide, and anilide substrates. The kcat/Km values were compatible with those of chymotrypsin [EC 3.4.21.1] with a few exceptions. One ionizable group with a pK of around 5.8 was suggested to be involved in the free enzyme in hydrolyzing all... | Kinetic studies of carboxypeptidase Y. III. Action on ester, amide, and anilide substrates and the effects of some environmental factors. Kinetic parameters of carboxypeptidase Y are given for the hydrolyses of ester, amide, and anilide substrates. The kcat/Km values were compatible with those of chymotrypsin [EC 3.4.2... | [
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PMID:5417 | Developmental changes in the structure and kinetic properties of myosin adenosinetriphosphatase of rabbit skeletal fast muscle. | Structural and functional changes in myosin of fast muscles during early post-natal development were studied to seek correlations with well-known physiological changes in the contraction rate. The findings were as follows: 1. It is known that fetal fast muscle myosin contains three kinds of light chains. It was confirm... | Developmental changes in the structure and kinetic properties of myosin adenosinetriphosphatase of rabbit skeletal fast muscle. Structural and functional changes in myosin of fast muscles during early post-natal development were studied to seek correlations with well-known physiological changes in the contraction rate.... | [
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PMID:5418 | Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions. | EDTA at a concentration of 1 muM produced a threshold effect in the activation of purified rabbit liver fructose-1, 6-diphosphatase [EC 3.1.3.11] in the presence of 5 mM Mg2+ at pH 7.2. Without EDTA, biphasic activation curves were produced by Mg2+. A double-reciprocal plot of the data gave the Km values corresponding ... | Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions. EDTA at a concentration of 1 muM produced a threshold effect in the activation of purified rabbit liver fructose-1, 6-diphosphatase [EC 3.1.3.11] in the presence of 5 mM Mg2+ at pH 7.2. Without EDTA, biphasic activation curves were produced... | [
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PMID:5419 | Two molecular forms of thymidine kinase in the cytosol of regenerating rat liver. | Two forms (Peak A and Peak B) of thymidine kinase [EC 2.7.1.75] from regenerating rat liver cytosol were resolved and partially purified by Deae-cellulose chromatography. Both fractions were identical with respect to their substrate requirement, pH optima, metal requirements, and molecular weight, as judged by their se... | Two molecular forms of thymidine kinase in the cytosol of regenerating rat liver. Two forms (Peak A and Peak B) of thymidine kinase [EC 2.7.1.75] from regenerating rat liver cytosol were resolved and partially purified by Deae-cellulose chromatography. Both fractions were identical with respect to their substrate requi... | [
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PMID:5420 | The modification of sulfhydryl groups of glutamine synthetase from Bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid). | The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the molecule as well as the effect of the modification on the enzyme activity. Three SH groups per subunit were detected after com... | The modification of sulfhydryl groups of glutamine synthetase from Bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid). The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH gro... | [
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PMID:5421 | Titration study of acetylated lysozyme. | To study the interaction between carboxyl groups and amino groups in native lysozyme [EC 3.2.1.17], and to identify the positions and the pK values of the abnormal carboxyl groups, N-acetylated lysozyme was prepared. The acetylation did not affect the molecular shape of the enzyme, but changed six amino groups to a non... | Titration study of acetylated lysozyme. To study the interaction between carboxyl groups and amino groups in native lysozyme [EC 3.2.1.17], and to identify the positions and the pK values of the abnormal carboxyl groups, N-acetylated lysozyme was prepared. The acetylation did not affect the molecular shape of the enzym... | [
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PMID:5422 | Isolation of a low molecular weight active fragment of potato proteinase inhibitor IIb. | A low molecular weight active fragment of potato proteinase inhibitor IIPB was obtained by incubating the inhibitor with an equimolar amount of trypsin [EC 3.4.21.4] at pH 8 and 30 degrees for 16 hr, followed by gel filtration through Sephadex G-50, treatment with trichloroacetic acid, and CM-cellulose chromatography. ... | Isolation of a low molecular weight active fragment of potato proteinase inhibitor IIb. A low molecular weight active fragment of potato proteinase inhibitor IIPB was obtained by incubating the inhibitor with an equimolar amount of trypsin [EC 3.4.21.4] at pH 8 and 30 degrees for 16 hr, followed by gel filtration throu... | [
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PMID:5423 | Isolation and characterization of a proteinase from the sarcocarp of melon fruit. | A proteinase from the sarcocarp of melon (Cucumis Melo L. var. Prince) was purified by a three-step procedure involving batch-wise treatment with CM-cellulose fibers, column chromatography on CM-cellulose powder and gel filtration on Sephadex G-75. The final enzyme preparation was homogeneous on acrylamide gel electrop... | Isolation and characterization of a proteinase from the sarcocarp of melon fruit. A proteinase from the sarcocarp of melon (Cucumis Melo L. var. Prince) was purified by a three-step procedure involving batch-wise treatment with CM-cellulose fibers, column chromatography on CM-cellulose powder and gel filtration on Seph... | [
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PMID:5424 | D-alpha-Hydroxyglutarate dehydrogenase of Rhodospirillum rubrum. | D-alpha-Hydroxyglutarate dehydrogenase of R. rubrum grown anaerobically in the light was partially purified and some properties were investigated. 1. The enzyme catalyze stoichiometrically the dehydrogenation reaction of D-alpha-hydroxyglutarate into alpha-oxoglutarate, coupled with the reduction of 2, 6-dichlorophenol... | D-alpha-Hydroxyglutarate dehydrogenase of Rhodospirillum rubrum. D-alpha-Hydroxyglutarate dehydrogenase of R. rubrum grown anaerobically in the light was partially purified and some properties were investigated. 1. The enzyme catalyze stoichiometrically the dehydrogenation reaction of D-alpha-hydroxyglutarate into alph... | [
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PMID:5425 | Effects of pH indicators on various activities of chromatophroes of Rhodospirillum rubrum. | 1. The effects of pH indicators on activities for ATP hydrolysis in the dark and ATP-Pi exchange in the dark were examined with chromatophores from Rhodospirillum rubrum. Of thirty-one pH indicators tested, eleven (metanil yellow, 2, 4-dinitrophenol, ethyl orange, bromocresol green, resazurin, neutral red, bromthymol b... | Effects of pH indicators on various activities of chromatophroes of Rhodospirillum rubrum. 1. The effects of pH indicators on activities for ATP hydrolysis in the dark and ATP-Pi exchange in the dark were examined with chromatophores from Rhodospirillum rubrum. Of thirty-one pH indicators tested, eleven (metanil yellow... | [
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PMID:5426 | Binding of substrate analogues to subsites D, E, and F of hen egg-white lysozyme. | The pH dependence of the binding of dye, Beibrich Scarlet, to hen egg-white lysozyme[EC 3.2.1.17] was studied at ionic strength 0.3 and 25 degrees by following circular dichroic (CD)bands originating from the bound dye. This binding involved one of the catalytic groups, Glu 35. The effect of the binding of N-acetylgluc... | Binding of substrate analogues to subsites D, E, and F of hen egg-white lysozyme. The pH dependence of the binding of dye, Beibrich Scarlet, to hen egg-white lysozyme[EC 3.2.1.17] was studied at ionic strength 0.3 and 25 degrees by following circular dichroic (CD)bands originating from the bound dye. This binding invol... | [
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PMID:5427 | Purification and characterization of alkaline phosphatase from rat kidney. | Alkaline phosphatase [EC 3.1.3.1.] was purified about 250-fold from rat kidney, and its enzymological properties were studied. Kidney homogenate was extracted with n-butanol, passed through Sephadex G-200 and chromatographed on a DEAE-cellulose column. The peak from the DEAE-cellulose column was subjected to isoelectri... | Purification and characterization of alkaline phosphatase from rat kidney. Alkaline phosphatase [EC 3.1.3.1.] was purified about 250-fold from rat kidney, and its enzymological properties were studied. Kidney homogenate was extracted with n-butanol, passed through Sephadex G-200 and chromatographed on a DEAE-cellulose ... | [
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PMID:5428 | Studies on a microchemical method for the determination of the degree of polymerization of neutral oligo- and polysaccharides. I. Quantitative separation of trace amounts of alditols from mixtures with a large excess of monosaccharides. | A method has been devised for the quantitative separation of trace amounts of alditols from mixtures with large amounts of monosaccharides, using a strongly basic ion-exchange resin. Ten kinds of common reducing monosaccharides used were strongly retained by a very basic anion-exchange resin in the hydroxyl form, where... | Studies on a microchemical method for the determination of the degree of polymerization of neutral oligo- and polysaccharides. I. Quantitative separation of trace amounts of alditols from mixtures with a large excess of monosaccharides. A method has been devised for the quantitative separation of trace amounts of aldit... | [
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PMID:5429 | Studies on barley trypsin inhibitor. II. Structural changes induced by denaturants and their reversibility. | No change in the activity of a trypsin inhibitor from barley was observed on treatment with heat and denaturants such as urea and guanidine hydrochloride. Thus, the conformational properties of the inhibitor were investigated. CD spectra of the native inhibitor were analysed on a curve-fitting technique using the data ... | Studies on barley trypsin inhibitor. II. Structural changes induced by denaturants and their reversibility. No change in the activity of a trypsin inhibitor from barley was observed on treatment with heat and denaturants such as urea and guanidine hydrochloride. Thus, the conformational properties of the inhibitor were... | [
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PMID:5430 | Isolation and some properties of NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii. | NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 38... | Isolation and some properties of NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii. NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filt... | [
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PMID:5431 | Interaction of cyclodextrins with fluorescent probes and its application to kinetic studies of amylase. | It was found that 6-p-toluidinylnaphthalene-2-sulfonate (TNS) showed pronounced fluorescence enhancement when it was added to alpha-, beta-, and gamma-cyclodextrin solutions. 2. The following results were obtained by quantitative study of the interactions of three kinds of cyclodextrins with TNS by following TNS fluore... | Interaction of cyclodextrins with fluorescent probes and its application to kinetic studies of amylase. It was found that 6-p-toluidinylnaphthalene-2-sulfonate (TNS) showed pronounced fluorescence enhancement when it was added to alpha-, beta-, and gamma-cyclodextrin solutions. 2. The following results were obtained by... | [
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PMID:5432 | Fluorine-19 as a covalent active site-directed magnetic resonance probe in aspartate transaminase. | Phosphypyridoxyl trifluoroethylamine has been synthesized as an active site-directed 19F NMR probe for aspartate transaminase. This coenzyme derivative adds stoichiometrically to the apotransaminase as observed by both fluorescence and circular dichroism measurements. The fluorinated phosphypyridoxamine derivative, whe... | Fluorine-19 as a covalent active site-directed magnetic resonance probe in aspartate transaminase. Phosphypyridoxyl trifluoroethylamine has been synthesized as an active site-directed 19F NMR probe for aspartate transaminase. This coenzyme derivative adds stoichiometrically to the apotransaminase as observed by both fl... | [
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PMID:5433 | Spot hemoglobin. Studies on the Root effect hemoglobin of a marine teleost. | The Spot, Leiostomus xanthrus, has a single tetrameric hemoglobin. Structural studies indicate the presence of alpha- and beta-like chains with COOH-terminal sequences of --Arg and --TYR-His, respectively, the same as is found in human hemoglobin. Spot hemoglobin possesses a Root effect: a heterotropic control mechanis... | Spot hemoglobin. Studies on the Root effect hemoglobin of a marine teleost. The Spot, Leiostomus xanthrus, has a single tetrameric hemoglobin. Structural studies indicate the presence of alpha- and beta-like chains with COOH-terminal sequences of --Arg and --TYR-His, respectively, the same as is found in human hemoglob... | [
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PMID:5434 | Site-site interactions among insulin receptors. Characterization of the negative cooperativity. | By studying the dissociation of 125I-instulin from its receptors in the absence and phe negatively cooperative type for the insulin receptors. In the present study we extend oy purified mouse and rat liver membranes as well as in human circulating monocytes and human cultured lymphocytes demonstrated negative cooperati... | Site-site interactions among insulin receptors. Characterization of the negative cooperativity. By studying the dissociation of 125I-instulin from its receptors in the absence and phe negatively cooperative type for the insulin receptors. In the present study we extend oy purified mouse and rat liver membranes as well ... | [
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PMID:5435 | Rabbit skeletal muscle glycogen synthase. II. Enzyme phosphorylation state and effector concentrations as interacting control parameters. | The effects of several inhibitors (ATP, ADP, AMP, UDP, and P1) and activators (Mg2+, glucose-6-P) of rabbit muscle glycogen synthase (UDP-glucose:glycogen 4-alpha-glucosyltransferase, EC 2.4.1.11) were studied in relation to the phosphorylation state of the purified enzyme. All the modifiers had increasing effects with... | Rabbit skeletal muscle glycogen synthase. II. Enzyme phosphorylation state and effector concentrations as interacting control parameters. The effects of several inhibitors (ATP, ADP, AMP, UDP, and P1) and activators (Mg2+, glucose-6-P) of rabbit muscle glycogen synthase (UDP-glucose:glycogen 4-alpha-glucosyltransferase... | [
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PMID:5436 | Kinetic study of the action of snake venom phospholipase A2 on human serum high density lipoprotein 3. | The hydrolysis of the phospholipids of intact human serum high density lipoprotein 3 (HDL3) by pure alpha-phospholipase A2 from Crotalus adamanteus was studied by pH-stat titration. The enzyme quantitatively hydrolyzed phosphatidylcholine and phosphatidylethanolamine and left sphinogomyelin intact, yielding a stable an... | Kinetic study of the action of snake venom phospholipase A2 on human serum high density lipoprotein 3. The hydrolysis of the phospholipids of intact human serum high density lipoprotein 3 (HDL3) by pure alpha-phospholipase A2 from Crotalus adamanteus was studied by pH-stat titration. The enzyme quantitatively hydrolyze... | [
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PMID:5437 | Regulation of steady state level of phosphoenzyme and ATP synthesis in sarcoplasmic reticulum vesicles during reversal of the Ca2+ pump. | The role of the Ca2+ concentration gradient in ATP synthesis and membrane phosphorylation by Pi was investigated in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. The Pi concentration required to attain 50% of the maximal membrane phosphorylation varies significantly in the pH range of 5.5 to 4.5... | Regulation of steady state level of phosphoenzyme and ATP synthesis in sarcoplasmic reticulum vesicles during reversal of the Ca2+ pump. The role of the Ca2+ concentration gradient in ATP synthesis and membrane phosphorylation by Pi was investigated in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscl... | [
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... |
PMID:5438 | Acetyl coenzyme A carbosylase. Circular dichroism studies of Escherichia coli biotin carboxyl carrier protein. | The biotin carboxyl carrier protein (BCCP) component of Escherichia coli acetyl coenzyme A carboxylase and three peptides derived from BCCP by proteolytic digestion have been examined by circular dichroism spectroscopy. BCCP, which has a peptide molecular weight of 22,500, has a spectrum typical of globular proteins wi... | Acetyl coenzyme A carbosylase. Circular dichroism studies of Escherichia coli biotin carboxyl carrier protein. The biotin carboxyl carrier protein (BCCP) component of Escherichia coli acetyl coenzyme A carboxylase and three peptides derived from BCCP by proteolytic digestion have been examined by circular dichroism spe... | [
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PMID:5439 | Pulse radiolytic investigation of single heme group reduction in hum an methemoglobin. | Reduction of one of the four heme groups of human aquomethemoglobin A has been investigated by the pulse radiolysis method. The reactivity of e-a-q, the hydrated electron, with methemoglobin was determined by observing this species directly. The separate reactions of the hydroxy yl radical and hydrogen atom, as well as... | Pulse radiolytic investigation of single heme group reduction in hum an methemoglobin. Reduction of one of the four heme groups of human aquomethemoglobin A has been investigated by the pulse radiolysis method. The reactivity of e-a-q, the hydrated electron, with methemoglobin was determined by observing this species d... | [
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PMID:5440 | Enzymatic attack on side chains of synthetic polymers. Chymotrypsin-catalyzed hydrolysis of specific substrate groups attached to acrylamide or acrylic acid co-polymers. | Three vinyl monomers, M-1, M-3, and M-5, in which L-phenylalanine p-nitroanilide was acylated with CH2==CHCONH(CH2)nCO--(n = 1, 3, 5) were synthesized. They were co-polymerized with a large excess of acrylamide (co-polymers PAm-1, PAm-3, and PAm-5) and with a large excess of acrylic acid (co-polymers PAc=1, PAc-3, and ... | Enzymatic attack on side chains of synthetic polymers. Chymotrypsin-catalyzed hydrolysis of specific substrate groups attached to acrylamide or acrylic acid co-polymers. Three vinyl monomers, M-1, M-3, and M-5, in which L-phenylalanine p-nitroanilide was acylated with CH2==CHCONH(CH2)nCO--(n = 1, 3, 5) were synthesized... | [
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PMID:5441 | Purification and properties of a highly potent antitumor glutaminase-asparaginase from Pseudomonas 7Z. | Crystalline glutaminase-asparaginase which is effective against solid as well as ascites tumors was prepared from soil isolate organism Pseudomonas 7A. This enzyme has a ration of Vmax for L-glutamine and L-asparagine of 2.0. The presence of glutamic acid in the growth medium is essential for optimal enzyme production ... | Purification and properties of a highly potent antitumor glutaminase-asparaginase from Pseudomonas 7Z. Crystalline glutaminase-asparaginase which is effective against solid as well as ascites tumors was prepared from soil isolate organism Pseudomonas 7A. This enzyme has a ration of Vmax for L-glutamine and L-asparagine... | [
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PMID:5442 | Regulation of secretion from the adrenal medulla. Evidence for adenylate cyclase activity in secretory vesicle membranes. | Adenylate cyclase activity has been found in purified secretory vesicle membranes from the adrenal medulla. Activity was detected both by formation of radioactive cAMP from [alpha-32P]ATP and by the competitive protein binding assay for cAMP. Activity was highest at pH 8.0 to 8.5, and was stimulated by sodium fluoride ... | Regulation of secretion from the adrenal medulla. Evidence for adenylate cyclase activity in secretory vesicle membranes. Adenylate cyclase activity has been found in purified secretory vesicle membranes from the adrenal medulla. Activity was detected both by formation of radioactive cAMP from [alpha-32P]ATP and by the... | [
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PMID:5443 | In vivo inhibition of superoxide dismutase in mice by diethyldithiocarbamate. | Superoxide dismutase was assayed by a method which takes advantage of the inhibitory action of superoxide dismutase (or tissues which contain superoxide dismutase) on the rate of autooxidation of 6-hydroxydopamine. Incubation of pure superoxide dismutase of homogenates of brain or liver with 10(-3) M diethyldithiocarba... | In vivo inhibition of superoxide dismutase in mice by diethyldithiocarbamate. Superoxide dismutase was assayed by a method which takes advantage of the inhibitory action of superoxide dismutase (or tissues which contain superoxide dismutase) on the rate of autooxidation of 6-hydroxydopamine. Incubation of pure superoxi... | [
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PMID:5444 | Internal pH of isolated chromaffin vesicles. | The passive permeability of isolated chromaffin vesicles to H+ and the internal pH of the vesicles under various conditions were measured. Potentiometric measurements of K+ and H+ fluxes in the presence of selected ionophores and uncouplers indicated that the membrane is highly impermeable to both protons and potassium... | Internal pH of isolated chromaffin vesicles. The passive permeability of isolated chromaffin vesicles to H+ and the internal pH of the vesicles under various conditions were measured. Potentiometric measurements of K+ and H+ fluxes in the presence of selected ionophores and uncouplers indicated that the membrane is hig... | [
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PMID:5445 | Triglyceride, diglyceride, monoglyceride, and cholesterol ester hydrolases in chicken adipose tissue activated by adenosine 3':5'-Monophosphate-dependent protein kinase. Chromatographic resolution and immunochemical differentiation from lipoprotein lipase. | Hormone-sensitive lipase and cholesterol ester hydrolase of chicken adipose tissue were markedly activated by adenosine 3':5'-monophosphate (cAMP)-dependent protein kinase (on the average, 235 to 275%; occasionally as much as 1000%). Diglyceride and monoglyceride hydrolases were also activated, but to a lesser extent (... | Triglyceride, diglyceride, monoglyceride, and cholesterol ester hydrolases in chicken adipose tissue activated by adenosine 3':5'-Monophosphate-dependent protein kinase. Chromatographic resolution and immunochemical differentiation from lipoprotein lipase. Hormone-sensitive lipase and cholesterol ester hydrolase of chi... | [
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PMID:5446 | Binding studies of polypeptide hormones to bovine neurophysins. | Experimental binding isotherms of [9-glycinamide-1-(14)C]oxytocin and [9-glycinamide-1-(14)C]arginine vasopressin to purified neurophysins I and II at pH = 4.4, 5.4, 6.5, 7.4, and 8.5 and 6 degrees, 22 degrees, and 37 degrees in aqueous buffers are reported. For purposes of comparison, binding isotherms for [4-glycine-... | Binding studies of polypeptide hormones to bovine neurophysins. Experimental binding isotherms of [9-glycinamide-1-(14)C]oxytocin and [9-glycinamide-1-(14)C]arginine vasopressin to purified neurophysins I and II at pH = 4.4, 5.4, 6.5, 7.4, and 8.5 and 6 degrees, 22 degrees, and 37 degrees in aqueous buffers are reporte... | [
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PMID:5447 | Inhibition of bovine hepatic fructose-1,6-diphosphatase by substrate analogs. | Purified bovine hepatic fructose-1,6-diphosphatase, which exhibits maximal activity at neutral pH, is competitively inhibited by several analogs of its substrate, fructose 1,6-diphosphate. These include glucose 1,6-diphosphate (Ki = 9.4 X 10(-5) M), hexitol 1,6-diphosphate (Ki = 2.3 X 10(-4) M), and 2,5-anhydro-D-manni... | Inhibition of bovine hepatic fructose-1,6-diphosphatase by substrate analogs. Purified bovine hepatic fructose-1,6-diphosphatase, which exhibits maximal activity at neutral pH, is competitively inhibited by several analogs of its substrate, fructose 1,6-diphosphate. These include glucose 1,6-diphosphate (Ki = 9.4 X 10(... | [
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PMID:5448 | Genetic variants of human erythrocyte glucose-6-phosphate dehydrogenase. Kinetic and thermodynamic parameters of variants A, B, and A- in relation to quaternary structure. | The values of Vmax and Km for the three genetic variants A, B, and A- of erythrocyte glucose-6-phosphate dehydrogenase have been determined at 10 different pH values in the range from 5.5 to 9.5, and at four different temperatures in the range from 18.5-40.0 degrees. The log Vmax versus pH curve for each of the enzymes... | Genetic variants of human erythrocyte glucose-6-phosphate dehydrogenase. Kinetic and thermodynamic parameters of variants A, B, and A- in relation to quaternary structure. The values of Vmax and Km for the three genetic variants A, B, and A- of erythrocyte glucose-6-phosphate dehydrogenase have been determined at 10 di... | [
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PMID:5449 | Studies on tyrosine residues in porcine muscle adenylate kinase. Circular dichroism spectra and chemical modification with tetranitromethane. | Substrate-induced conformational change of porcine muscle adenylate kinase (EC 2.7.4.3) is evidenced by a change in circular dichroism spectra in the near ultraviolet. In the absence of tryptophan in porcine muscle adenylate kinase, the spectral change may be assigned to a perturbation of tyrosine chromophore(s). The s... | Studies on tyrosine residues in porcine muscle adenylate kinase. Circular dichroism spectra and chemical modification with tetranitromethane. Substrate-induced conformational change of porcine muscle adenylate kinase (EC 2.7.4.3) is evidenced by a change in circular dichroism spectra in the near ultraviolet. In the abs... | [
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PMID:5450 | Antifreeze glycoproteins from Antarctic fish. Inactivation by borate. | Antifreeze glycoprotein, which has previously been shown to be inactive in the presence of borate, migrates electrophoretically as the borate complex, presumably through formation of borate complexes with hydroxyl groups on the sugar side chains. Antifreeze glycoprotein (5 mg/ml) has been found to be completely active ... | Antifreeze glycoproteins from Antarctic fish. Inactivation by borate. Antifreeze glycoprotein, which has previously been shown to be inactive in the presence of borate, migrates electrophoretically as the borate complex, presumably through formation of borate complexes with hydroxyl groups on the sugar side chains. Ant... | [
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PMID:5451 | Binding of (3H)prostaglandin E1 to putative receptors linked to adenylate cyclase of cultured cell clones. | A method for assessing the binding of 3H-labeled prostaglandin E1 ([3H]PGE1) to cell membranes has been developed and used to study the interaction of [3H]PGE1 with membranes from cultured mammalian cells. Receptor sites were identified by correlation of the potency of a series of compounds to compete for [3H]PGE1 bind... | Binding of (3H)prostaglandin E1 to putative receptors linked to adenylate cyclase of cultured cell clones. A method for assessing the binding of 3H-labeled prostaglandin E1 ([3H]PGE1) to cell membranes has been developed and used to study the interaction of [3H]PGE1 with membranes from cultured mammalian cells. Recepto... | [
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PMID:5452 | The mechanism of superoxide anion generation by the interaction of phenylhydrazine with hemoglobin. | The mechanism by which superoxide anion is generated by the interaction of phenylhydrazine with either oxy- or methemoglobin was investigated. Rather than superoxide anion generation resulting from an accelerated autooxidation of oxyhemoglobin, it was found that both oxy- and methemoglobin function as peroxidases towar... | The mechanism of superoxide anion generation by the interaction of phenylhydrazine with hemoglobin. The mechanism by which superoxide anion is generated by the interaction of phenylhydrazine with either oxy- or methemoglobin was investigated. Rather than superoxide anion generation resulting from an accelerated autooxi... | [
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0.071... |
PMID:5453 | Affinity labeling of a previously undetected essential lysyl residue in class I fructose bisphosphate aldolase. | The affinity label N-bromoacetylethanolamine phosphate (BrAcNHEtOP) has been used previously at pH 6.5 to identify His-359 of rabbit muscle aldolase as an active site residue. We now find that the specificity of the reagent is pH-dependent. At pH 8.5, alkylation with 14C-labeled BrAcNHEtOP abolishes both fructose-1,6-P... | Affinity labeling of a previously undetected essential lysyl residue in class I fructose bisphosphate aldolase. The affinity label N-bromoacetylethanolamine phosphate (BrAcNHEtOP) has been used previously at pH 6.5 to identify His-359 of rabbit muscle aldolase as an active site residue. We now find that the specificity... | [
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PMID:5454 | Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus. | A repressible alkaline phosphatase has been isolated from the extreme bacterial thermophile, Thermus aquaticus. The enzyme can be derepressed more than 1,000-fold by starving the cells for phosphate. In derepressed cells, nearly 6% of the total protein in a cell-free enzyme preparation is alkaline phosphatase. The enzy... | Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus. A repressible alkaline phosphatase has been isolated from the extreme bacterial thermophile, Thermus aquaticus. The enzyme can be derepressed more than 1,000-fold by starving the cells for phosphate. In derepressed cells, ne... | [
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PMID:5455 | Association of gylcogenolysis with cardiac sarcoplasmic reticulum. | Sarcoplasmic reticulum fragments isolated from dog cardiac muscle possess a calcium-accumulating system associated with a series of enzymes linked to glycogenolysis. These enzymes include: adenylate cyclase, cyclic AMP-dependent protein kinase, phosphorylase b kinase, phosphorylase (b/a, 30/1),"debrancher" enzyme, and ... | Association of gylcogenolysis with cardiac sarcoplasmic reticulum. Sarcoplasmic reticulum fragments isolated from dog cardiac muscle possess a calcium-accumulating system associated with a series of enzymes linked to glycogenolysis. These enzymes include: adenylate cyclase, cyclic AMP-dependent protein kinase, phosphor... | [
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PMID:5456 | Purification and properties of a virion protein kinase. | The protein kinase associated with virions of frog virus 3 was purified to apparent homogeneity by ion exchange chromatography and gel filtration. The enzyme protein appeared as a single polypeptide of molecular weight 50,000 to 55,000 as determined by gel filtration, glycerol gradient sedimentation, and sodium dodecyl... | Purification and properties of a virion protein kinase. The protein kinase associated with virions of frog virus 3 was purified to apparent homogeneity by ion exchange chromatography and gel filtration. The enzyme protein appeared as a single polypeptide of molecular weight 50,000 to 55,000 as determined by gel filtrat... | [
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0.00... |
PMID:5457 | The interaction of macromolecular solutions with macromolecular monolayers adsorbed on a hydrophobic surface. | In order to elucidate the general patterns of intermacromolecular surface interactions that may be involved in hemocompatibility phenomena, monolayers of representative macromolecules on an octadecylsilylated glass surface were exposed to solutions of other macromolecules, and the changes in interfacial composition wer... | The interaction of macromolecular solutions with macromolecular monolayers adsorbed on a hydrophobic surface. In order to elucidate the general patterns of intermacromolecular surface interactions that may be involved in hemocompatibility phenomena, monolayers of representative macromolecules on an octadecylsilylated g... | [
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0.... |
PMID:5458 | Stimulation of ornithine decarboxylase activity in chick fibroblasts by non-suppressible insulin-like activity (NSILA), insulin and serum. | A factor isolated from human serum (nonsuppressible insulin-like activity, NSILA) stimulates multiplication of serum-starved chick embryo fibroblasts and stimulates activity of ornithine decarboxylase (ODC). Physiological doses of NSILA (200 muU/ml) and pharmacological doses of insulin (200 mU/ml) stimulate ODC 4-5-fol... | Stimulation of ornithine decarboxylase activity in chick fibroblasts by non-suppressible insulin-like activity (NSILA), insulin and serum. A factor isolated from human serum (nonsuppressible insulin-like activity, NSILA) stimulates multiplication of serum-starved chick embryo fibroblasts and stimulates activity of orni... | [
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PMID:5459 | Gas-liquid chromotographic determination of perazine, thioridazine and thioridazine metabolites in human plasma. | A gas-liquid chromatographic method for the detection of perazine, thioridazine and its major metabolites in human plasma is presented. Repeated extraction, an internal standard and a temperature program with flame ionization detection make possible accurate and reproducible results with patients on therapeutic doses o... | Gas-liquid chromotographic determination of perazine, thioridazine and thioridazine metabolites in human plasma. A gas-liquid chromatographic method for the detection of perazine, thioridazine and its major metabolites in human plasma is presented. Repeated extraction, an internal standard and a temperature program wit... | [
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PMID:5460 | A reversed-phase thin-layer chromotographic method for the determination of relative partition coefficients of very lipophilic compounds. | A reversed-phase thin-layer chromatographic method has been developed for the determination of partition coefficients. A support phase has been chosen, following investigation of the lack of adsorptive properties, which has a minimal effect on the pH of the buffer system. A stationary phase has been chosen to give delt... | A reversed-phase thin-layer chromotographic method for the determination of relative partition coefficients of very lipophilic compounds. A reversed-phase thin-layer chromatographic method has been developed for the determination of partition coefficients. A support phase has been chosen, following investigation of the... | [
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PMID:5461 | High-pressure liquid chromatography of drugs. II. An evaluation of a microparticulate cation-exchange column. | A microparticulate cation-exchange column has been evaluated for the chromatography of thirty compounds selected as representative of a wide variety of drug substances. Although the column exhibited a strong partition effect besides the expected ion-exchange mechanism, the retention of drugs could be predictably influe... | High-pressure liquid chromatography of drugs. II. An evaluation of a microparticulate cation-exchange column. A microparticulate cation-exchange column has been evaluated for the chromatography of thirty compounds selected as representative of a wide variety of drug substances. Although the column exhibited a strong pa... | [
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PMID:5462 | Resolution in affinity chromatography. The effect of the heterogeneity of immobilized soybean trypsin inhibitor on the separation of pancreatic proteases. | By affinity chromatography, trypsins and chymotrypsins from mouse pancreas homogenates have been separated using soybean trypsin inhibitor immobilized on Sepharose. The effects of the functional heterogeneity of the adsorbent have been investigated in terms of the resolution obtained. Heterogeneity of the adsorbent hav... | Resolution in affinity chromatography. The effect of the heterogeneity of immobilized soybean trypsin inhibitor on the separation of pancreatic proteases. By affinity chromatography, trypsins and chymotrypsins from mouse pancreas homogenates have been separated using soybean trypsin inhibitor immobilized on Sepharose. ... | [
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PMID:5463 | A thin-layer chromatographic method for the determination of acebutolol and its major metabolite in serum. | A sensitive and specific thin-layer chromatographic method for the simultaneous determination of acebutolol [DL-1-(2-acetyl-4-n-butyramidophenoxy)-2-hydroxy-3-isopropylaminopropane] and its major metabolite [DL-1-(2-acetyl-4-acetamidophenoxy)-2-hydroxy-3-isopropylaminopropane] is described. A 2-ml volume of serum with ... | A thin-layer chromatographic method for the determination of acebutolol and its major metabolite in serum. A sensitive and specific thin-layer chromatographic method for the simultaneous determination of acebutolol [DL-1-(2-acetyl-4-n-butyramidophenoxy)-2-hydroxy-3-isopropylaminopropane] and its major metabolite [DL-1-... | [
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PMID:5465 | Influence of L-prolyl-L-leucyl-glycine amide on growth hormone secretion in normal and acromegalic subjects. | Melanocyte Release-Inhibiting Peptide (MRIP-I) did not affect circulating levels of ACTH, LH, FSH, TSH,ORL, betaMSH and insulin when iv infused (5.0 mg in 5 min plus 0.4 mg/min for 70-115 min), while it significantly reduced serum GH response to hypoglycemia in normal subjects and lowered serum GH levels in acromegalic... | Influence of L-prolyl-L-leucyl-glycine amide on growth hormone secretion in normal and acromegalic subjects. Melanocyte Release-Inhibiting Peptide (MRIP-I) did not affect circulating levels of ACTH, LH, FSH, TSH,ORL, betaMSH and insulin when iv infused (5.0 mg in 5 min plus 0.4 mg/min for 70-115 min), while it signific... | [
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PMID:5466 | Use of Counter and rocket immunoelectrophoresis in acute respiratory infections due to Streptococcus pneumoniae. | The use of Counter immunoelectrophoresis (CIE) for the detection of pneumococcal capsular antigen in the sputum and serum of patients suffering from acute respiratory infections is described. The CIE of sputum gave positive results in 224 (99%) out of 225 samples in which Streptococcus pneumoniae was isolated by cultur... | Use of Counter and rocket immunoelectrophoresis in acute respiratory infections due to Streptococcus pneumoniae. The use of Counter immunoelectrophoresis (CIE) for the detection of pneumococcal capsular antigen in the sputum and serum of patients suffering from acute respiratory infections is described. The CIE of sput... | [
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PMID:5467 | Requirement for a macromolecular factor for sodium azide activation of guanulate cyclase. | Sodium azide, a highly nucleophilic agent and a potent metabolic inhibitor, markedly increased guanylate cyclase activity from supernatant fractions of rat liver homogenates. The effect of sodium azide was not observed with partially purified guanulate cyclase from liver or crude soluble guanylate cyclase from cerebral... | Requirement for a macromolecular factor for sodium azide activation of guanulate cyclase. Sodium azide, a highly nucleophilic agent and a potent metabolic inhibitor, markedly increased guanylate cyclase activity from supernatant fractions of rat liver homogenates. The effect of sodium azide was not observed with partia... | [
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PMID:5469 | A rapid method for the assay of guanylate cyclase. | An extremely rapid and sensitive assay for guanylate cyclase utilizing [alpha-32P]-GTP has been developed. It involves incubation of 5-100 mug of enzyme protein with 1 mM [alpha-32P]-GTP in 40 mM Tris HC1 buffer (pH 7.4) containing 3-3 mM MnSO2, 10 mM theophylline and 1 mM cyclic GMP. The reaction is terminated by addi... | A rapid method for the assay of guanylate cyclase. An extremely rapid and sensitive assay for guanylate cyclase utilizing [alpha-32P]-GTP has been developed. It involves incubation of 5-100 mug of enzyme protein with 1 mM [alpha-32P]-GTP in 40 mM Tris HC1 buffer (pH 7.4) containing 3-3 mM MnSO2, 10 mM theophylline and ... | [
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PMID:5468 | Lysosomal enzyme secretion from human neutrophils mediated by cyclic CMP: inhibition of cyclic GMP accumulation and neutrophil function by glucocorticosteroids. | The effects of several glucocorticosteroids on cyclic GMP accumulation, guanylate cyclase activity, calcium influx, lysosomal enzyme secretion, and phagocytosis were studied in human neutrophils. Contact between neutrophils and serum-treated zymosan particles, in the presence of calcium at pH 7.4, triggered these cellu... | Lysosomal enzyme secretion from human neutrophils mediated by cyclic CMP: inhibition of cyclic GMP accumulation and neutrophil function by glucocorticosteroids. The effects of several glucocorticosteroids on cyclic GMP accumulation, guanylate cyclase activity, calcium influx, lysosomal enzyme secretion, and phagocytosi... | [
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PMID:5470 | Cyclic AMP content and regulation of tyrosine-3-mono-oxygenase in rat striatum. | The unilateral transection of nigro striatal dopaminergic axons produced a short lasting (15-30 min) increase in the affinity of striatal tyrosine 3-mono-oxygenase (TH), for a synthetic pteridine cofactor (DMPH4). The kinetic changes of striatal TH were paralleled by an increase of striatal cAMP content. Apomorphine (1... | Cyclic AMP content and regulation of tyrosine-3-mono-oxygenase in rat striatum. The unilateral transection of nigro striatal dopaminergic axons produced a short lasting (15-30 min) increase in the affinity of striatal tyrosine 3-mono-oxygenase (TH), for a synthetic pteridine cofactor (DMPH4). The kinetic changes of str... | [
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PMID:5471 | Properties of guanylate cyclase from rat kidney cortex and transplantable kidney tumors. | The subcellular distribution and properties of guanylate cyclase was examined in preparations of normal rat renal cortex and Morris renal tumors MK2 and MK3. In normal kidney cortex about two-thirds of guanylate cyclase activity of homogenates was found in soluble fractions. With renal tumors the homogenate activity wa... | Properties of guanylate cyclase from rat kidney cortex and transplantable kidney tumors. The subcellular distribution and properties of guanylate cyclase was examined in preparations of normal rat renal cortex and Morris renal tumors MK2 and MK3. In normal kidney cortex about two-thirds of guanylate cyclase activity of... | [
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PMID:5472 | Reduction of enamel solubility by sodium monophosphate. | The preponderance of evidence indicates that sodium monofluorophosphate exerts a highly beneficial effect on dental caries incidence and enamel solubility. Optimal effects are obtained with a concentration of 4 X 10(3) ppm fluoride, by a four-minute application and by adjusting the pH to 4.0. The reaction of sodium mon... | Reduction of enamel solubility by sodium monophosphate. The preponderance of evidence indicates that sodium monofluorophosphate exerts a highly beneficial effect on dental caries incidence and enamel solubility. Optimal effects are obtained with a concentration of 4 X 10(3) ppm fluoride, by a four-minute application an... | [
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PMID:5478 | Proxemics, locus of control, anxiety, and type of movement in emotionally disturbed and normal boys. | In order to determine the interpersonal distancing requirements for emotionally disturbed and normal children and in order to investigate the relationship of locus of control and anxiety to interpersonal space, 20 emotionally disturbed and 20 normal boys were randomly required to approach an object person and to let th... | Proxemics, locus of control, anxiety, and type of movement in emotionally disturbed and normal boys. In order to determine the interpersonal distancing requirements for emotionally disturbed and normal children and in order to investigate the relationship of locus of control and anxiety to interpersonal space, 20 emoti... | [
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PMID:5482 | Pharmacotherapy in older depressed patients. | Treatment of depression in elderly patients is not generically different from treatment of depression in younger age cohorts. Because of certain age-related physical, physiological, and biochemical factors, however, drug prescription for geriatric patients must be modified in several respects. Tricyclic antidepressants... | Pharmacotherapy in older depressed patients. Treatment of depression in elderly patients is not generically different from treatment of depression in younger age cohorts. Because of certain age-related physical, physiological, and biochemical factors, however, drug prescription for geriatric patients must be modified i... | [
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PMID:5560 | The binding of complement by complexes formed between a rabbit antibody and oligosaccharides of increasing size. | Immune complexes formed between a homogeneous rabbit antibody to type III pneumococcal polysaccharide and a series of oligosaccharides of varying size derived from it were prepared and tested for their ability to fix guinea pig hemolytic complement. Antibody and either tetra-, hexa-, or octasaccharide formed only monom... | The binding of complement by complexes formed between a rabbit antibody and oligosaccharides of increasing size. Immune complexes formed between a homogeneous rabbit antibody to type III pneumococcal polysaccharide and a series of oligosaccharides of varying size derived from it were prepared and tested for their abili... | [
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PMID:5561 | Neonatal infection with mouse thymic virus: effects on cells regulating the antibody response to type III pneumococcal polysaccharide. | Mice infected neonatally with mouse thymic virus (TA) were evaluated at different ages with respect to their ability to give a plaque-forming cell (PFC) response to type III pneumococcal polysaccharide (SSS-III), as well as the degree of amplifier and suppressor thymus-derived (T) cell activity present. B cell activity... | Neonatal infection with mouse thymic virus: effects on cells regulating the antibody response to type III pneumococcal polysaccharide. Mice infected neonatally with mouse thymic virus (TA) were evaluated at different ages with respect to their ability to give a plaque-forming cell (PFC) response to type III pneumococca... | [
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PMID:5483 | The chromosomes of four species of the nasuta complex of Drosophila. I. Chromosome maps and inversion polymorphism. | The salivary chromosomes of four species of the nasuta complex of Drosophila, D. sulfurigaster albostrigata, D, kohkoa, D. albomicans, and D. kepulauana were studied and chromosome maps of each species are presented; the maps of the latter three species are based on the map of D. sulfurigaster albostrigata. Three of th... | The chromosomes of four species of the nasuta complex of Drosophila. I. Chromosome maps and inversion polymorphism. The salivary chromosomes of four species of the nasuta complex of Drosophila, D. sulfurigaster albostrigata, D, kohkoa, D. albomicans, and D. kepulauana were studied and chromosome maps of each species ar... | [
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PMID:5562 | Graft-vs-host reactions in F1 mice induced by parental lymphoid cells: nature of recruited F1 cells. | Graft versus host (GVH) reactivity of parental lymph node (LN) cells was assayed by measurements of 3H-thymidine incorporation in vivo. Mitomycin (Mit.) treatment of parental cells abolished their proliferative activity but the combination of such Mit.-treated parental cells with F1 LN cells resulted in much higher pro... | Graft-vs-host reactions in F1 mice induced by parental lymphoid cells: nature of recruited F1 cells. Graft versus host (GVH) reactivity of parental lymph node (LN) cells was assayed by measurements of 3H-thymidine incorporation in vivo. Mitomycin (Mit.) treatment of parental cells abolished their proliferative activity... | [
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PMID:5563 | Determinants of red cell sickling. Effects of varying pH and of increasing intracellular hemoglobin concentration by osmotic shrinkage. | The effects of varying pH and of increasing intracellular hemoglobin (Hb) concentration on red cell sickling and oxygen affinity were studied in whole blood from persons with sickle cell anemia (SS) and sickle cell trait (SA). Small increases in SS blood pH inhibited sickling, and small reductions in both SS and SA blo... | Determinants of red cell sickling. Effects of varying pH and of increasing intracellular hemoglobin concentration by osmotic shrinkage. The effects of varying pH and of increasing intracellular hemoglobin (Hb) concentration on red cell sickling and oxygen affinity were studied in whole blood from persons with sickle ce... | [
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PMID:5564 | Bacterial interference as a factor in renal infection. | These experiments have investigated the role of bacterial interference as a determinant in the epidemiology of renal infection. Two unrelated strains of Escherichia coli, E. coli 08 and 075, isolated from cases of clinical pyelonephritis were used. Although both strains had identical morphology on conventional media th... | Bacterial interference as a factor in renal infection. These experiments have investigated the role of bacterial interference as a determinant in the epidemiology of renal infection. Two unrelated strains of Escherichia coli, E. coli 08 and 075, isolated from cases of clinical pyelonephritis were used. Although both st... | [
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PMID:5565 | The effect of the plasma bicarbonate level on proximal tubule sodium reabsorption in NH4Cl-loaded dogs. | In an attempt to examine the effects of mild and severe chronic metabolic acidosis on proximal tubule sodium reabsorption, 6 dogs were given 10 mEq. per kilogram per day and 5 dogs were given 20mEq. per kilogram per day of ammonium chloride for 3 days and compared to 12 normal dogs during a steady-state water diuresis ... | The effect of the plasma bicarbonate level on proximal tubule sodium reabsorption in NH4Cl-loaded dogs. In an attempt to examine the effects of mild and severe chronic metabolic acidosis on proximal tubule sodium reabsorption, 6 dogs were given 10 mEq. per kilogram per day and 5 dogs were given 20mEq. per kilogram per ... | [
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PMID:5566 | Lethal midline granuloma: a pathological spectrum. | Lethal midline granuloma is a non-specific clinical term used for different pathological entities often exhibiting some semblance to each other as they lie along a histological spectrum. Nevertheless, each entity has a specific diagnostic pattern. The histological picture with distinguishing features of each specific t... | Lethal midline granuloma: a pathological spectrum. Lethal midline granuloma is a non-specific clinical term used for different pathological entities often exhibiting some semblance to each other as they lie along a histological spectrum. Nevertheless, each entity has a specific diagnostic pattern. The histological pict... | [
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PMID:5567 | Ganglioside biosynthesis. Characterization of uridine diphosphate galactose: GM2 galactosyltransferase in golgi apparatus from rat liver. | An enzyme that transfers galactose from UDP-Gal to ganglioside GM2 (Tay-Sachs ganglioside) was concentrated 50 times in Golgi apparatus from rat liver relative to total homogenates. This enzyme required detergents or phospholipids as dispersing agents. Of the numerous detergents tested, sodium taurocholate and Triton C... | Ganglioside biosynthesis. Characterization of uridine diphosphate galactose: GM2 galactosyltransferase in golgi apparatus from rat liver. An enzyme that transfers galactose from UDP-Gal to ganglioside GM2 (Tay-Sachs ganglioside) was concentrated 50 times in Golgi apparatus from rat liver relative to total homogenates. ... | [
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PMID:5568 | Effect on various putative neurotransmitters on the secretion of corticotrophin-releasing hormone from the rat hypothalamus in vitro-a model of the neurotransmitters involved. | The effect of incubating the hypothalamus of adult male rats with various neurotransmitters upon the release of corticotrophin-releasing hormone (CRH) was studied. The CRH activity in the incubation medium was assayed in 48 h median eminence-lesioned rats and the corticosteroidogenesis of excised adrenals in vitro was ... | Effect on various putative neurotransmitters on the secretion of corticotrophin-releasing hormone from the rat hypothalamus in vitro-a model of the neurotransmitters involved. The effect of incubating the hypothalamus of adult male rats with various neurotransmitters upon the release of corticotrophin-releasing hormone... | [
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PMID:5569 | Purification of canine prolactin by preparative isotachophoresis. | Preparative isotachophoresis in polyacrylamide gel using carrier ampholytes as 'spacers' has been used to purify prolactin from canine pituitary extracts. Using Ampholine pH 5-8 as spacers, a prolactin fraction was obtained which was essentially homogeneous as judged by the criteria of disc electrophoresis and isoelect... | Purification of canine prolactin by preparative isotachophoresis. Preparative isotachophoresis in polyacrylamide gel using carrier ampholytes as 'spacers' has been used to purify prolactin from canine pituitary extracts. Using Ampholine pH 5-8 as spacers, a prolactin fraction was obtained which was essentially homogene... | [
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PMID:5570 | Acid-base balance in rainbow trout (Salmo gairdneri) subjected to acid stresses. | 1. The respiratory properties of rainbow-trout blood were investigated in acid-stressed fish. In the first group acid was introduced into the bloodstream and in the second the carbon dioxide content of the ambient water was increased. 2. Initially the introduction of acid to the blood caused a decrease in blood pH and ... | Acid-base balance in rainbow trout (Salmo gairdneri) subjected to acid stresses. 1. The respiratory properties of rainbow-trout blood were investigated in acid-stressed fish. In the first group acid was introduced into the bloodstream and in the second the carbon dioxide content of the ambient water was increased. 2. I... | [
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PMID:5571 | Connexions between hair-plate afferents and motoneurones in the cockroach leg. | 1. The trochanteral hair-plate afferents in the metathoracic leg of the cockroach, Periplaneta americana, were stimulated electrically and at the same time intracellular recordings were made from either motoneurones, interneurones or afferent terminals within the methathoracic ganglion. 2. Activity in the hair-plate af... | Connexions between hair-plate afferents and motoneurones in the cockroach leg. 1. The trochanteral hair-plate afferents in the metathoracic leg of the cockroach, Periplaneta americana, were stimulated electrically and at the same time intracellular recordings were made from either motoneurones, interneurones or afferen... | [
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PMID:5572 | Physiological properties of eel haemoglobin: hypoxic acclimation, phosphate effects and multiplicity. | Unlike the whole body oxygen affinity, which adapts readily to environmental oxygen tensions, haemoglobins, prepared from normoxic- and hypoxic-accimated eels (Anguilla anguilla) show no adaptive changes in oxygenation properties or in multiplicity. Hypoxic acclimation is, howeveer, accompanied by a strong decrease in ... | Physiological properties of eel haemoglobin: hypoxic acclimation, phosphate effects and multiplicity. Unlike the whole body oxygen affinity, which adapts readily to environmental oxygen tensions, haemoglobins, prepared from normoxic- and hypoxic-accimated eels (Anguilla anguilla) show no adaptive changes in oxygenation... | [
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PMID:5573 | [LP-X in newborns: increased incidence of positive tests without cholestasis (author's transl)]. | The investigation of 194 newborns has shown that during the first weeks of life the abnormal lipoprotein-X (LP-X) was present in the serum of nearly 50% of the infants, with no clinical chemical evidence of cholestasis. The percentage of LP-X positive tests was even higher in the group of immature newborns (65%). There... | [LP-X in newborns: increased incidence of positive tests without cholestasis (author's transl)]. The investigation of 194 newborns has shown that during the first weeks of life the abnormal lipoprotein-X (LP-X) was present in the serum of nearly 50% of the infants, with no clinical chemical evidence of cholestasis. The... | [
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PMID:5574 | Neurotrophic activity of brain extracts in forelimb regeneration of the urodele, Triturus. | The loss in protein synthesis which the regenerating forelimb of the newt suffers after denervation can be recovered by infusing into it an extract of newt soluble brain protein. Moreover, the synthesis of basic protein shows a greater response to the active brain principle than does that of acidic protein. The active ... | Neurotrophic activity of brain extracts in forelimb regeneration of the urodele, Triturus. The loss in protein synthesis which the regenerating forelimb of the newt suffers after denervation can be recovered by infusing into it an extract of newt soluble brain protein. Moreover, the synthesis of basic protein shows a g... | [
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PMID:5575 | Interaction between phloretin and the red blood cell membrane. | Phloretin binding to red blood cell components has been characterized at pH6, where binding and inhibitory potency are maximal. Binding to intact red cells and to purified hemoglobin are nonsaturated processes approximately equal in magnitude, which strongly suggests that most of the red cell binding may be ascribed to... | Interaction between phloretin and the red blood cell membrane. Phloretin binding to red blood cell components has been characterized at pH6, where binding and inhibitory potency are maximal. Binding to intact red cells and to purified hemoglobin are nonsaturated processes approximately equal in magnitude, which strongl... | [
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PMID:5576 | Effect of synthetic substance P on monoaminergic mechanisms in brain. | Synthetic substance P has been discovered to stimulate significantly the formation of dopa in the limbic, striatum, hemisphere and diencephalon regions of the brain and the lower brain stem. There was no effect upon 5-hydroxytryptophan formation or on tryptophan or tyrosine levels. After inhibition of monoamine synthes... | Effect of synthetic substance P on monoaminergic mechanisms in brain. Synthetic substance P has been discovered to stimulate significantly the formation of dopa in the limbic, striatum, hemisphere and diencephalon regions of the brain and the lower brain stem. There was no effect upon 5-hydroxytryptophan formation or o... | [
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PMID:5580 | Gamma-glutamyl transpeptidase. Elevated activity in myotonic dystrophy. | gamma-Glutamyl transpeptidase, a membrane-bound enzyme playing an important role in the active amino acid transport across cellular membranes, is shown to be elevated in the serum of patients with myotonic muscular dystrophy. No increase of AP, LAP, GOT and GPT activities in the sera of some of the patients studied is ... | Gamma-glutamyl transpeptidase. Elevated activity in myotonic dystrophy. gamma-Glutamyl transpeptidase, a membrane-bound enzyme playing an important role in the active amino acid transport across cellular membranes, is shown to be elevated in the serum of patients with myotonic muscular dystrophy. No increase of AP, LAP... | [
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PMID:5581 | Occurrence of a trypsin inhibitor in eggplant exocarps. | A trypsin inhibitor was extracted from eggplant exocarps with several buffers. The 0.1 M acetate buffer, pH 5.5. extract had the highest specific activity. The crude inhibitor, obtained by heat treatment and salting-out from the acetate buffer extract, contained 4.5% nitrogen and 22.6% hexose. Isoelectrofocusing demons... | Occurrence of a trypsin inhibitor in eggplant exocarps. A trypsin inhibitor was extracted from eggplant exocarps with several buffers. The 0.1 M acetate buffer, pH 5.5. extract had the highest specific activity. The crude inhibitor, obtained by heat treatment and salting-out from the acetate buffer extract, contained 4... | [
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PMID:5583 | Ruptured coronary aneurysm and valvulitis in an infant with polyarteritis nodosa. | This report describes a 4-mth-old infant with polyarteritis nodosa who had a rapidly fatal course with the diagnosis ultimately being made at necropsy. The pathological lesions consisted of diffuse arterial disease with severe coronary involvement, and additional unusual features included a ruptured coronary artery ane... | Ruptured coronary aneurysm and valvulitis in an infant with polyarteritis nodosa. This report describes a 4-mth-old infant with polyarteritis nodosa who had a rapidly fatal course with the diagnosis ultimately being made at necropsy. The pathological lesions consisted of diffuse arterial disease with severe coronary in... | [
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PMID:5584 | Mannosidosis: phenotype of a severely affected child and characterization of alpha-mannosidase activity in cultured fibroblasts from the patient and his parents. | A three-year-old boy has coarse facial features, upper respiratory congestion, profound mental retardation, hepatosplenomegaly, increased height and head circumference, cataracts, a gibbus deformity, radiographic changes of dysostosis multiplex, and vacuolized peripheral lymphocytes. These findings are the most commonl... | Mannosidosis: phenotype of a severely affected child and characterization of alpha-mannosidase activity in cultured fibroblasts from the patient and his parents. A three-year-old boy has coarse facial features, upper respiratory congestion, profound mental retardation, hepatosplenomegaly, increased height and head circ... | [
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PMID:5585 | Maternal and fetal plasma levels of free corticosteroids in pathological deliveries. | Does intrauterine acidosis induce increased steroid secretion? The concentration of free steroids (CS) increases in both fetal and maternal plasma during labor and delivery. Fetal levels are higher after vaginal than after cesarean section. These differences may indicate an important role of the fetal adrenal gland in ... | Maternal and fetal plasma levels of free corticosteroids in pathological deliveries. Does intrauterine acidosis induce increased steroid secretion? The concentration of free steroids (CS) increases in both fetal and maternal plasma during labor and delivery. Fetal levels are higher after vaginal than after cesarean sec... | [
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PMID:5588 | Gastric acid inactivation of erythromycin stearate in solid dosage forms. | The effect of hydrochloric acid at pH 1.2-3.2 ON ERYTHROMYCIN STEARATE AND COMMERCIAL DOSAGE FORMS OF ERYTHROMYCIN STEARATE WAS STUDIED. Under all conditions examined, erythromycin was readily dissolved from the stearate as hydrochloride, and rapidly lost its biological activity in solution. The inclusion of pepsin in ... | Gastric acid inactivation of erythromycin stearate in solid dosage forms. The effect of hydrochloric acid at pH 1.2-3.2 ON ERYTHROMYCIN STEARATE AND COMMERCIAL DOSAGE FORMS OF ERYTHROMYCIN STEARATE WAS STUDIED. Under all conditions examined, erythromycin was readily dissolved from the stearate as hydrochloride, and rap... | [
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PMID:5589 | Synthesis and alpha-adrenolytic activity of chiral beta-haloethylamines. | Several series of N,N-diaralkyl- and N-aryloxyalkyl-N-aralkyl-beta-haloethylamines were synthesized containing centers of chirality in the aralkyl substituent and in the beta-haloethyl moiety to study the stereoselectivity of alpha-adrenolytic activity of the beta-haloethylamines. These compounds were synthesized from ... | Synthesis and alpha-adrenolytic activity of chiral beta-haloethylamines. Several series of N,N-diaralkyl- and N-aryloxyalkyl-N-aralkyl-beta-haloethylamines were synthesized containing centers of chirality in the aralkyl substituent and in the beta-haloethyl moiety to study the stereoselectivity of alpha-adrenolytic act... | [
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PMID:5590 | Rapid GLC determination of fusaric acid in biological fluids. | A simple, sensitive GLC assay was developed for fusaric acid, the active metabolite of bupicomide, to follow the disposition of this investigational antihypertensive agent in patients undergoing therapy. Fusaric acid is efficiently extracted from biological samples, derivatized by on-column methylation, and chromatogra... | Rapid GLC determination of fusaric acid in biological fluids. A simple, sensitive GLC assay was developed for fusaric acid, the active metabolite of bupicomide, to follow the disposition of this investigational antihypertensive agent in patients undergoing therapy. Fusaric acid is efficiently extracted from biological ... | [
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PMID:5591 | Conformational populations for antihistamines and antihypertensives in solution. | The conformations in aqueous solution were determined for the XCH2CH2N systems of some antihistamines (H1-receptor antagonists) and some adrenergic neuron blocking agents, including the antihypertensive drugs guanethidine and guanoclor. The rotameric species thought to be the pharmacologically active one is often not t... | Conformational populations for antihistamines and antihypertensives in solution. The conformations in aqueous solution were determined for the XCH2CH2N systems of some antihistamines (H1-receptor antagonists) and some adrenergic neuron blocking agents, including the antihypertensive drugs guanethidine and guanoclor. Th... | [
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PMID:5592 | Determination of hyoscyamine in BPC mixtures. | The hyoscyamine contents of four BPC mixtures (containing either belladonna or hyoscyamus tincture) were determined using the acid-dye technique. A sample size of 10 ml was required. The mean percentage recovery of hyoscyamine ranged from 99.73 to 101.03 from three mixtures; from the magnesium trisilicate and belladonn... | Determination of hyoscyamine in BPC mixtures. The hyoscyamine contents of four BPC mixtures (containing either belladonna or hyoscyamus tincture) were determined using the acid-dye technique. A sample size of 10 ml was required. The mean percentage recovery of hyoscyamine ranged from 99.73 to 101.03 from three mixtures... | [
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PMID:5594 | Use of 300-msec microwave irradiation for enzyme inactivation: a study of effects of sodium pentobarbital on acetylcholine concentration in mouse brain regions. | Microwave irradiation of 6 kw at 2450 MHz for 300 msec was sufficient to completely inactivate mouse brain cholinesterase and choline acetyltransferase. After this method of sacrifice, the acetylcholine contents of mouse brain regions, given in nanomoles per gram, were found to be: striatum, 81; medulla-pons, 44; dienc... | Use of 300-msec microwave irradiation for enzyme inactivation: a study of effects of sodium pentobarbital on acetylcholine concentration in mouse brain regions. Microwave irradiation of 6 kw at 2450 MHz for 300 msec was sufficient to completely inactivate mouse brain cholinesterase and choline acetyltransferase. After ... | [
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PMID:5596 | A test for antinociceptive activity of narcotic and narcotic antagonist analgesics in the guinea pig. | Ethylenediamine tetraacetic acid (EDTA) was used as a nociceptive stimulus intradermally in the guinea pig. The responses evoked by EDTA included vocalization, biting and scratching at the site of injection and escape behavior. Nociception by EDTA was shown to be the result of its cation chelating activity. The suppres... | A test for antinociceptive activity of narcotic and narcotic antagonist analgesics in the guinea pig. Ethylenediamine tetraacetic acid (EDTA) was used as a nociceptive stimulus intradermally in the guinea pig. The responses evoked by EDTA included vocalization, biting and scratching at the site of injection and escape ... | [
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PMID:5597 | Pharmacological characterization of adrenergic receptors of a rabbit cerebral artery in vitro. | In the light of controversy over the functional significance of the abundant sympathetic innervation of large cerebral blood vessels, an in vitro analysis of the adrenergic receptors mediating contractile effects in the rabbit basilar artery was undertaken. Beta adrenergic stimulation had no effect, and agents that blo... | Pharmacological characterization of adrenergic receptors of a rabbit cerebral artery in vitro. In the light of controversy over the functional significance of the abundant sympathetic innervation of large cerebral blood vessels, an in vitro analysis of the adrenergic receptors mediating contractile effects in the rabbi... | [
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PMID:5598 | Cation-binding property of choroid plexus peptide IIF. | Preparations of the melanotropic-lipolytic peptide IIF from bovine choroid plexus contain 3% Ca, 1% Mg and less than 0.1% Na and K. Ca and Mg were removed by gel filtration on Sephadex G-10 in 1 N acetic acid. Capacity of the resulting cation-free peptide to bind Ca++, Mg++, Na+ and K+ was examined with the method of H... | Cation-binding property of choroid plexus peptide IIF. Preparations of the melanotropic-lipolytic peptide IIF from bovine choroid plexus contain 3% Ca, 1% Mg and less than 0.1% Na and K. Ca and Mg were removed by gel filtration on Sephadex G-10 in 1 N acetic acid. Capacity of the resulting cation-free peptide to bind C... | [
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PMID:5599 | A study of the mechanism of transport of diphenylhydantoin in the rat submaxillary gland in vitro. | In vitro studies performed on rat submaxillary gland slices with diphenylhydantoin (6 X 10(-6) M) showed that variations of extracellular pH (pHe) had no significant effects on uptake and only slight effects on efflux (lowering the pHe slightly decreased efflux rates, whereas increasing pHe slightly increased efflux ra... | A study of the mechanism of transport of diphenylhydantoin in the rat submaxillary gland in vitro. In vitro studies performed on rat submaxillary gland slices with diphenylhydantoin (6 X 10(-6) M) showed that variations of extracellular pH (pHe) had no significant effects on uptake and only slight effects on efflux (lo... | [
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PMID:5600 | Aminopyridines and sparteine as inhibitors of membrane potassium conductance: effects on Myxicola giant axons and the lobster neuromuscular junction. | The effects of the compounds 2-, 3- and 4-aminopyridine and sparteine on membrane conductance changes were examined using both voltage-clamped Myxicola axons and the lobster neuromuscular junction. In Myxicola axons, the aminopyridines very specifically inhibited the potassium conductance when applied at concentrations... | Aminopyridines and sparteine as inhibitors of membrane potassium conductance: effects on Myxicola giant axons and the lobster neuromuscular junction. The effects of the compounds 2-, 3- and 4-aminopyridine and sparteine on membrane conductance changes were examined using both voltage-clamped Myxicola axons and the lobs... | [
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PMID:5601 | Effects of calcium on the local anesthetic suppression of ionic conductances in squid axon membranes. | The effects of varying the external calcium concentration on the suppression of membrane ionic conductances by procaine and benzocaine have been examined under voltage-clamped conditions. The suppression of peak conductance and steady-state conductance by procaine or benzocaine applied externally or internally was not ... | Effects of calcium on the local anesthetic suppression of ionic conductances in squid axon membranes. The effects of varying the external calcium concentration on the suppression of membrane ionic conductances by procaine and benzocaine have been examined under voltage-clamped conditions. The suppression of peak conduc... | [
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PMID:5602 | Twitch potentiation of skeletal muscle by physostigmine at different pH. | This report extends earlier research, done with external media at pH 7.2, to new studies at pH 6.4 and 8.4 as well as 7.2, to determine the roles of the protonated and neutral forms of physostigmine (a weak base with pKalpha = 8.2) in causing twitch potentiation of the frog sartorius muscle. Physostigmine, especially a... | Twitch potentiation of skeletal muscle by physostigmine at different pH. This report extends earlier research, done with external media at pH 7.2, to new studies at pH 6.4 and 8.4 as well as 7.2, to determine the roles of the protonated and neutral forms of physostigmine (a weak base with pKalpha = 8.2) in causing twit... | [
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PMID:5603 | The renal blood flow and the glomerular filtration rate of anaesthetized dogs during acute changes in plasma sodium concentration. | 1. The effects of acute changes in plasma Na concentration (P(Na)) on renal blood flow (RBF) and glomerular filtration rate (GFR) were studied in anaesthetized greyhounds. Saline was infused at a constant rate (0.1 ml. kg(-1) min(-1)) either into a renal artery or into a systemic vein. Plasma Na concentration was alter... | The renal blood flow and the glomerular filtration rate of anaesthetized dogs during acute changes in plasma sodium concentration. 1. The effects of acute changes in plasma Na concentration (P(Na)) on renal blood flow (RBF) and glomerular filtration rate (GFR) were studied in anaesthetized greyhounds. Saline was infuse... | [
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PMID:5604 | Hydrolytic enzymes of Euglena gracilis: characterization and activity as a function of culture age and carbon deprivation. | Optimal assay conditions are described for 8 hydrolases of Euglena gracilis var. bacillaris, SM-L1 (streptomycin-bleached) strain, 7 of which have an acid pH-optimum. Acid-phosphatase, beta-galactosidase, beta-glucosidase, b-fucosidase, cathepsin D, RNase, DNase, and an esterase are active in cell homogenates. Amylase ... | Hydrolytic enzymes of Euglena gracilis: characterization and activity as a function of culture age and carbon deprivation. Optimal assay conditions are described for 8 hydrolases of Euglena gracilis var. bacillaris, SM-L1 (streptomycin-bleached) strain, 7 of which have an acid pH-optimum. Acid-phosphatase, beta-galacto... | [
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PMID:5607 | Adrenergic sulfonanilides. 4. Centrally active beta-adrenergic agonists. | The central nervous system (CNS) activities of a number of soterenol analogs have been investigated, and several of these compounds possessed potent morphine antagonistic and anorexiant properties. The CNS activity of these compounds was enhanced by certain lipophilic [e.g., 1,1-dimethyl-2-phenethyl (43) or cyclopropyl... | Adrenergic sulfonanilides. 4. Centrally active beta-adrenergic agonists. The central nervous system (CNS) activities of a number of soterenol analogs have been investigated, and several of these compounds possessed potent morphine antagonistic and anorexiant properties. The CNS activity of these compounds was enhanced ... | [
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PMID:5611 | Ontogenetic development of NADH-dependent methemoglobin reductase in erythrocytes of man and rat. | Ontogenetic development of NADH-dependent methemoglobin reductase was followed in humans and rats. The human kinetic profile differs from that in the rat. The low level of methemoglobin reductase in human infants at birth and for the first months of life may provide a partial explanation of the particular susceptibilit... | Ontogenetic development of NADH-dependent methemoglobin reductase in erythrocytes of man and rat. Ontogenetic development of NADH-dependent methemoglobin reductase was followed in humans and rats. The human kinetic profile differs from that in the rat. The low level of methemoglobin reductase in human infants at birth ... | [
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0.07... |
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