Datasets:
OTAR3088
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BioData-CellLine_annotated

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PMC11746942
Additionally, CD31 (Servicebio, GB11063-3-100), P-Src (Y419) (Abways, CY5468), Src (Abcam, ab32102), and anti-GAPDH (Fude, FD0063) antibodies were used.
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PMC11726183
The synthetic procedure of compounds 5–25 is outlined in Scheme 1.
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PMC11794588
In a flask, 5 mL of complete media was added to trypsinized cells.
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PMC11707832
The missense mutation c.2248C>T (p.Arg750Trp) stands out as a common occurrence among α-mannosidosis patients, having been reported in the majority of European populations studied, comprising over 30% of all detected disease alleles .
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PMC9878562
Previous data showed that in zebrafish ACKR3 expressed in trans on stromal cells can support the CXCL12-mediated migration of primordial germ cells (44).
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PMC11644761
To assess the effect of pterostilbene on the cell cycle, we also performed dual-color indirect immunofluorescence (IIF) analysis to examine the presence or absence of the Ki67 proliferative marker and the AT8 epitope of the nuclear tau protein in relation to the physiological states of neuroblastoma cells.
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PMC3765139
The arrays were washed and stained on a Fluidics Station 450 (Affymetrix) by using a Hybridization Wash and Stain Kit (Affymetrix, Cat # 900720).
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PMC11352877
Vascular maturation occurs during the secretory phase of the menstrual cycle, which is regulated by progesterone.
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PMC11699042
Segmented data was used to calculate the Genomic Index (GI) as the square of the number of CN-altered DNA segments divided by the number of CN-altered chromosomes as previously described.
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PMC10747160
All samples were run in triplicate with at least two separate biological replicates for each condition.
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PMC10605143
Heat shock was carried out by placing plates with logarithmically growing cells in a water bath at a temperature of 43 °C for 1 h. Subsequently, cells were allowed to recover for the indicated time in a CO2 incubator at 37 °C.
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PMC11763111
F The plots show the results of flow cytometry analysis of the percentage of CAR-T cells and tumor cells in bone marrow cells collected from each mouse at the time of death (n = 5), the statistical significance was determined with one-way ANOVA.
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PMC9429973
Presenting clinical information was abstracted from review of unstructured notes as well as structured data from the foundry.
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PMC11525293
It has been reported that COVID-19 patients treated with Thal had a faster recovery in respect to untreated patients .
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PMC10748106
In some cases, the reactivity order of the compound related to the substitutions in C4 is as follows: any > hydroxymethyl > aldehyde (D55, D56, and D58).
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PMC10938377
Values are given as the mean ± SD (μM).
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PMC11774112
The drug loading rate (DL) and encapsulation efficiency (EE) were 29.25 ± 3.23 μg/mg and 78.3 ± 5.71%, respectively.
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PMC11676670
Ascitic fluid was collected in a sterile 50 mL tube and processed within an hour to sediment and isolate tumor cells.
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PMC10196713
Ovarian cancer (OC) is a refractory cancer that shows recurrence due to the acquisition of resistance to anticancer drugs, including cisplatin.
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PMC11471176
At the molecular level, the two conditions have numerous associations.
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PMC11755467
This may be in trend indicative that the anti-HIV-1 replicative effect of Enoxacin might at least partially be explained by the down-regulation of pro-HIV-1 hsa-miR-132-3p in CEM-SS cells (Figure 1a,b).
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PMC11540664
Lymphopenia is associated with poor prognosis in multiple cancer types and can be used to predict the efficacy of tumor immunotherapy (10–12).
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PMC11612315
One-way ANOVA followed by Dunnett’s multiple comparison test (ns, not significant; ****p < 0.0001, compared with the control, pH 5.5).
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PMC9874064
colonies area of cells formed by Image J. G) Live and dead assay of 3D tumor spheres after 48 ​h treatment of PBS, cisplatin, NP-Pt and NP-Pt-USP1i.
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PMC11001582
Neuropixels recordings (30-kHz sampling rate) were processed and automatically spike sorted using Kilosort3 using default parameters.
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PMC9503541
Although the active and inactive fractions were separated, it is possible that the analytes were co-eluted or that the fractions were not fully separated.
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PMC9429973
Low-dose decitabine-containing myeloablative conditioning regimens were administered in all cases.
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PMC9429973
S. Rives, S. L. Maude, H. Hiramatsu, A. Baruchel, P. Bader, H. Bittencourt, J. Buechner, T. Laetsch, B. De Moerloose, M. Qayed, H. E. Stefanski, K. L. Davis, P. L. Martin, E. Nemecek, C. Peters, G. Yanik, A. Balduzzi, N. Boissel, S. L. Khaw, J. Krueger, J. Levine, S. Davies, G. D. Myers, A. Yeo, D. O’Donovan, R. Ramos,...
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PMC9111184
3 Second derivative of FTIR spectra of Control and m-CA, ChA, RA, EZ treated cells in the spectral range 1300–1000 ​cm Thus, the changes in the DNA and protein regions can be explained by the effect of these compounds on some transcriptional factors and the regulation mRNA and protein levels.
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PMC11420784
PldA is sufficient for autophagy evasion by UPEC in the cytosol.
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PMC11609529
Co-transfection was performed with 200 ng YAP1/TEAD-luc (8xGTIIC-Luc), 10 ng Renilla luciferase control vector (p-RL), and 200 ng of empty pCMV2 vector, pCMV2-MAML2, pCMV2-YAP1, or pCMV2-YM plasmids.
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PMC11754784
Classical CCK-8 assay shows negligible cytotoxicity of DNPs (Supplementary Fig. 30), indicating the high biocompatibility of DNPs.
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PMC9596868
No. 05-636), mouse monoclonal anti-β-Actin clone AC-74 from Sigma-Aldrich (Cat.
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PMC11495567
The cytoskeleton comprises microtubules, actin filaments and intermediate filaments.
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PMC11756937
One day per week, “double feeding” was performed by exchanging the growth medium with 3 mL of fresh medium and not feeding the cells on the following day.
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PMC9444294
Here, we characterize a suite of molecular biology assays using this new antibody, both confirming existing and reporting on novel applications.
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PMC11708541
Additionally, it demonstrated appropriate pharmacokinetic and minimal toxicity (Fig. 13).
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PMC10940855
RNA was isolated using Qiagen RNeasy Micro kit (Qiagen, 74104).
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PMC10812665
The LUHMES are dopaminergic neuron precursor cells derived from a female human ventral mesencephalon and conditionally immortalized by v-myc.
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PMC11155445
Amongst the series compound (102i) followed by compound (102a) showed the highest antioxidant capacity of 87% and 44%, respectively, while all remaining compounds showed antioxidant capacity between 0 and 29% (Fig. 38).
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PMC3931643
AZD8055 was purchased from Active Biochem, PP242 and Rapamycin from LC labs (Woburn, MA).
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PMC11412721
Splenic cells were recovered from 6-week-old asymptomatic mice and incubated with DAPI and the following antibodies: APC rat anti-mouse CD45R/B220, FITC rat anti-mouse CD43, PE rat anti-mouse IgM, and BV605 rat anti-mouse IgD (BD Pharmingen).
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PMC10631132
A–H Following hematopoietic differentiation of PBMC-derived iPSCs A–D or fibroblast-derived iPSCs (E–H), either according to an embryoid body based (EB) A, B, E, F or a monolayer-based (MB) protocol (C, D, G, H), hematopoietic cells were matured with M-CSF and differentiated into OCs with RANKL on calcium-phosphate coa...
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PMC11489175
USP28 has been recently identified as a down-stream target gene of the β-catenin-YAP1-TBX5 transcriptional complex 97.
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PMC10968586
Other triple negative breast cancer cell lines were identified as particularly resistant towards HT-mediated cytotoxicity, e.g., SUM159 (IC50 = 300 μM) .
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PMC11194678
Untransfected cells were treated with rapamycin for 24 h, and fluorescence was measured with Leica TCS SP8 confocal microscope.
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PMC9429973
The immunophenotype of Sézary cells is heterogeneous and often varies among patients.
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PMC11320834
PARP1 is one of the key molecules involved in this process .
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PMC8193046
However, these ‘rejected putative positive substructures’ – which, nevertheless, resulted in class 7 molecules in a significant number – must be taken into special consideration for the further improvement of C@PA’s prediction capabilities (e.g., C@PA_1.X).
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PMC11359735
Published mass spectrometry-based proteomic analyses of sporozoites failed to detect peptides corresponding to the predicted GPI addition site (red arrow) and downstream residues, consistent with prior processing of the protein and removal of the putative GPI-addition sequences.
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PMC11754094
On the basis of the results of 60 non-targeting gRNAs, the Z-Score of large deletion for each gRNA was calculated.
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PMC11541409
Annals of Oncology, 2022.
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PMC11322866
This finding suggested that tight regulation of Lamin B1 level is essential, as both its deficiency and excess can regulate cellular senescence.
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PMC10165258
Within these ranges, 6 drug concentrations were tested for etoposide, and 7 for the cisplatin.
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PMC9429973
However, some patients may not be eligible to receive autologous CAR-T. TruUCAR™ GC502 is an allogeneic, universal CAR-T product with CD19/CD7 dual directed CAR.
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PMC11155445
Thermal behavior investigation of the synthesized Co(ii), Ni(ii), and Cu(ii) complexes showed that these compounds were thermally stable and remained unaffected by exposure to various temperatures like 165, 185, and 122 °C (Fig. 25).
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PMC6892818
c ACHN, 786-O and A-704 cells were treated with mCD40L for the indicated time periods (12 and 24 h) in the presence of 25 μM JNK inhibitor SP600125 or p38 inhibitor SB202190 and expression of Bak and Bax was detected in controls (‘C’) vs. mCD40L-treated cells (‘mL’) by immunoblotting (40 µg protein/lane).
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PMC7154001
Plasma levels of each metabolite were not significantly different in patients who received different doses of the drug.
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PMC11751675
They were first reported from artichoke; Cynara scolymus L. Grosheimol (1) and cynaropicrin (2) are strong inhibitors for HCV infection with a broad spectrum activity toward multiple HCV genotypes (1a, 1b, 2b, 3a, 4a, 5a, 6a, and 7a).
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PMC4355729
All mediums were supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 U/mL penicillin (Gibco) and 100 mg/mL streptomycin (Gibco), and incubated at 37°C with 5% CO2 in a humidified atmosphere.
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PMC9429973
ASXL1, IDH1/2 and SFSR2 mutations were associated with a trend in a higher response rate in the R/R group.
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PMC10813895
Furthermore, an indicator of biological activity from a prospective chemoprevention treatment could be a slowing of the rate of change of ELF5 expression or promoter methylation, as measured in RPFNAs (Figure 1C).
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PMC9429973
The median age was 58 years (ranging from 44 to 65).
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PMC11357960
After 24 hours of adding 2-D08, PBMCs were harvested and analyzed by flow cytometry.
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PMC11371627
We only included patients where sufficient clinical/phenotypic data was available for clinicogenetic correlation which left us with 44 cases amenable to our analysis.
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PMC11519077
After that, the aspirated blood sample was given an equivalent volume of PBS.
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PMC9429973
We developed a GMP protocol for NK cell ex vivo expansion in the presence of IL-2 and IL-15, and report the results of a phase 1 protocol of adoptive immunotherapy with activated and expanded autologous NK cells for Ph+ ALL patients in complete hematologic remission (CHR) but with persistent/recurrent minimal residual ...
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PMC11267830
The percentage of apoptotic primary cells increased significantly in the indisulam-treated group.
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PMC10582049
Using a variety of geometrical and textural features of lung tumors, the CANARY is able to distinguish in situ and invasive carcinomas, predict the invasiveness of the tumor, as well as LUAD patient survival and mortality at the early stage of progression.
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PMC9429973
The 107 of 8602 synonymous and other non-coding SNPs passed p-value threshold after multiple test correction (0.05/8602).
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PMC11767725
Focus: Articles that addressed metabolic and immunological factors influencing the skin microbiota and its role in skin diseases.
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PMC8973725
EMT can promote tumor metastasis and invasion by enhancing the mobility and reducing the adhesion of tumor cells .
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PMC10197932
Since survival of immune cells depends on anti-apoptotic Bcl-2 family members, we have evaluated the involvement of anti-apoptotic Mcl-1 protein.
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PMC4355729
It can promote the migration of different type of cells such as cancer cells35.
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PMC9429973
Additionally, a qPCR panel was conducted on CD41 cells isolated from untreated mouse BM to assess the TGF-β superfamily ligands and receptors that may be involved in regulating thrombopoiesis.
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PMC9878562
LTB4 ELISA was performed as indicated in the manufacturer’s instruction (Enzo Life Sciences).
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PMC9429973
Methods: We retrospectively reviewed a cohort of 101 consecutive patients with non-myeloid hematologic malignancies at Duke, comprising a total of 105 samples that were sent for CGP by FoundationOne Heme (104) or HemeComplete (1) in 2014–2021.
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PMC10978090
Therapeutic plasma exchange (TPE) has been proposed to remove heparin-induced thrombocytopenia (HIT) antibodies before planned thoracic surgery in patients with acute HIT and to allow brief re-exposure to heparin during surgery.
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PMC11320084
In cancer, mesenchymal features mediated by EMT enable cancer cells to complete multiple steps of metastasis, including local invasion, vascular invasion, metastasis through the circulatory system, and distant metastasis.
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PMC8973725
Briefly, 3 × 10 cells were seeded into the upper chamber of the insert with Matrigel (BD, Franklin Lakes, NJ, USA) in serum-free medium.
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PMC11739504
Acetylated α-tubulin and γ-tubulin were stained for primary cilia (green).
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PMC10878518
OBP-401 and OBP-301 have cytopathic effects and is against cancer cells in human .
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PMC9910796
In a first approach, we performed immunogold labeling with an anti-DNA antibody to quantify the percentage of control (+ IPTG) and defective (-IPTG) particles labeled at the budding areas (Fig 3D).
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PMC5839394
Metal nanoparticles are versatile agents with a variety of applications and in particular in nanomedicine for highly sensitive diagnostic assays, thermal ablation, radiotherapy enhancement including drug and gene delivery .
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PMC11536589
The IC50 of MRTX1133 treated for 72 h in MRTX1133-resistant PDAC cells. (
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PMC11694066
EWS–WT1, the unique oncogenic driver of desmoplastic small round cell tumors, confers sensitivity to PARP and ATR inhibitors, supporting the potential of these drugs in treating patients with this aggressive sarcoma subtype.
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PMC11422497
Tissues were collected during surgical operations and promptly stored in liquid nitrogen.
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PMC9429973
Four (45%) of them had received chemotherapy.
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PMC11621493
This broad substrate specificity allows the degradation of glycerolipids and their synthesis via acyl-CoA–independent mechanisms.
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PMC11322866
Representative photographs are shown.
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PMC9429973
K. Manola, P. Diamantopoulou, C. Kelaidi, L. Petrikkos, T. Ioannidou, K. Stefanaki, E. IOANNIDOU, N. Tourkantoni, K. Tsitsikas, M. Kourti, K. Antoniadi, E. Dana, I. Pelagiadis, E. Mantadakis, M. Economou, A. Makis, E. Papakonstantinou, H. Kosmidis, E. Stiakaki, G. Paterakis, A. Kattamis, I. Peristeri, D. Pantelia, M. I...
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PMC11766174
It should also be emphasized that the successful translation of Gag does not unequivocally confirm the generation of replication-competent progeny viral particles.
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PMC11767725
ML models using gut microbiome data from children have demonstrated an 87% accuracy in predicting caregiver-identified race and ethnicity, underscoring the role of structural disparities in shaping microbial communities.
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PMC11185260
Gene therapy, which involves replacement of a defective or missing gene with a functional, intact copy of that gene, is a potentially beneficial cancer treatment approach particularly over conventional chemotherapy, which often lacks selectivity and can cause non-specific toxicity.38 To date, gene therapy for cancer ha...
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PMC11718109
These blocks were further fixed with 1% glutaraldehyde in 120 mM PB, then washed thrice in 120 mM PB.
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PMC11536589
However, the expression levels of GPX4 showed no difference between AsPC1 cells and AsPC1-MR cells.
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PMC11699042
The A-673 EwS cell line was purchased from the American Type Culture Collection (ATCC).
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PMC11638288
When PD1.6 tumors were injected into mice with knocked-out NCR1 and wild-type mice, tumors in the NCR1 knockout mice grew normally, whereas in the wild-type mice, only initial tumor growth was observed, with complete tumor rejection over time .
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PMC11395280
CCR7-PE-Cy7 binding, which was not inhibited by either CCL19 or CCL21, on the surface of unstimulated cells was defined as 100% at t = 0.
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PMC3995603
The treatment with AC inhibited increases in plasma IgE levels (149.9 ± 22.66 ng/mL) in D. farinae-sensitized mice (P < 0.01).
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