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PMC11552389
After each incubation, sections were washed three times with Tris Buffered saline (TBS) for 10 min each.
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PMC11774747
A study highlighted the differences in memory potential based on the timing of CAR transduction (84).
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PMC11718809
Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of host genes of differentially expressed circRNAs and target genes within ceRNA networks was conducted using the KOBAS Web Tool with a hypergeometric test, while Gene Ontology (GO) analysis was conducted using the G:Profile Web Tool , and p-value correction was performed using Benjamini–Hochberg method.
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PMC11552389
Scale bar: 10 μm. **
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PMC11499955
Urine does not contain any traces of tags before [F]6 injection, indicating good in vivo stability of the antibody-tag conjugates (Fig. S19†).
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PMC11700340
Based on this fact, we hypothesized that the mechanism behind the anticancer properties of linagliptin is involved (cell cycle specific).
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PMC11634027
Based on this, we generated flies with genomic deletions of the D-II or D-III regions, and compared their phenotypes to those of ds and ds.
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PMC9429973
I. Lazana, E. Simantirakis, M. Daniil, I. Tsironis, G. Vassilopoulos Cell and Gene therapy, Biomedical Research Foundation of the Academy of Athens, Athens; Department of Hematology, University of Thessaly Medical School, Larissa, Greece Background: Anti-CD19 chimeric antigen receptor (CAR) T cells have transformed the field of cancer immunotherapy.
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PMC11742290
LGR5 + cells are responsible for self-renewal and differentiation into specialized cells, making them key players in both normal tissue regeneration and cancer, where they contribute to tumor growth and resistance to therapy.
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PMC6024716
Compared with the parental cells, higher concentration of intracellular polyamines was detected in the OAZ1 knockout cell lines; it was 2-fold higher in the 775–1 and 3.5-fold higher in the 775–3, with the most significant difference observed in the level of putrescine, where it was 10-fold higher in 775–1, and 88-fold higher in the 775–3 mutants.
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PMC9436459
The synthesis of dienophile S3 in a higher yield was completed by the Claisen–Schmidt condensation of S1 with S2 using NaH as the base in THF instead of conventional KOH/MeOH conditions.
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PMC11040965
P < .05.
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PMC11422497
Therefore, for the first time, we have identified and emphasized that circ_SMA4 is significantly upregulated and plays an oncogenic role in GISTs by sponging miR-494-3p to activate the KIT/JAK/STAT pathway.
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PMC11703992
The exposure genes were further validated in finn-b-GIANT_CELL_TEMP_ARTERITIS dataset to verify the significant causal effect to GCA risk.
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PMC11506670
First-round PCR was conducted using an external forward primer paired with an internal mutagenic reverse primer, and an internal mutagenic forward primer paired with an external reverse primer to generate the overlapping mutated left and right halves of NRAS, respectively.
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PMC11155445
The anti-inflammatory activity of seven novel 1,4-naphthoquinone acyl thiourea hybrids (135a–g) was investigated by evaluation of the production of 4 different cytokines (TNFα, IL6, IL12p40, GM-CSF) and was performed in presence and absence of LPS (Fig. 62).
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PMC11519583
b Cos7 cells were transfected with the indicated plasmids.
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PMC11679033
For that purpose, the numbers of mono-, bi-, tri-, and quadrinuclear cells (i.e., M1, M2, M3, M4) were scored at 400× magnification using a light microscope (Leitz, Oberkochen, Germany).
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PMC11490153
PBS containing 0.5% DMSO and AlgNPs (50 µl/well) were used as control and negative control groups.
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PMC11747467
However, the subtype heterogeneity of muscle-invasive bladder cancer (MIBC) often leads to different therapeutic outcomes.
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PMC9429973
Engrafted cells were used for serial transplantations.
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PMC9429973
Immunoblotting showed reduced GALE in platelets and significant reduction of LacNAc, suggesting a hypoglycosylation pattern.
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PMC11653533
Besides, it contains a natural product with a round shape, which contains high nutritive esteem; furthermore, the natural products are green and are around 2 to 3 cm in breadth, turning yellow or ruddy when they ripen.
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PMC11413393
A BD LSR2 analytical flow cytometer was used to acquire fluorescence data.
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PMC9429973
W. Macaron, E. Jabbour, M. Konopleva, F. Ravandi, N. Jain, G. Issa, T. Kadia, K. Sasaki, P. Kebriaei, M. Yilmaz, P. Thompson, K. Takahashi, H. Abbas, W. Wierda, R. Garris, H. Kantarjian, N. Short Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, United States of America Background: The development of novel monoclonal antibodies such inotuzumab ozogamicin (INO) and blinatumomab (Blina) results in superior response rates and survival in patients (pts) with relapsed/refractory (R/R) B-cell acute lymphoblastic leukemia (ALL).
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PMC1972402
It was concluded that the 636 bispecific antibody was highly effective in targeting the toxic moiety of the molecule to CEA-expressing cells, and allowed exploitation of the additional ability of the B chain to facilitate cellular incorporation.
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PMC11665584
SHH/PTCH1 signaling between HF matrix and dermal papilla cells is known to influence dermal papilla maturation and maintenance of the HF cycle.
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PMC11721295
Single-positive cells were related, and double-positive cells were classified.
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PMC6948907
Cell Culture and Determination of IC50 Value by MTT Assay The cells were cultured and grown in in DMEM containing 100μl /L FBS.
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PMC8753818
Further, results of this study demonstrated that caspase-7 deficiency had an adverse effect on cell cycle progression resulting in the reduction of cell proliferation .
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PMC9268620
The cytotoxicity was quantitatively estimated with a nonradioactive colorimetric assay system using tetrazolium salt (MTT, Sigma, St. Louis, MO, USA).
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PMC11679892
In addition, the potential of this herbal agent should be investigated in more detail with different doses, application times, and combined treatment strategies.
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PMC6468656
After indicated hours of incubation, upper surface of the filter was carefully removed with a cotton swab.
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PMC10033453
We also engineered a GFP-expressing VMG with which all sarcoma cell lines showed infection and varying degrees of GFP production 24 hpi (Figure S1).Figure 1VMG genome and cytotoxicity evaluation(A) Hybrid virus construct.
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PMC11756514
The bacteria were harvested by centrifugation in the THB cultures or single colonies picked from the CSA plates.
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PMC10968586
This piece of information sounds particularly alarming, since arsenic trioxide (As2O3) is one of the agents utilized to treat acute promyelocytic leukemia, especially in combinatory first-line therapy, and its mechanism of action relies on ROS increase .
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PMC9429973
7 patients did not complete all 6 cycles of HCQ-Kd, 5 due to PD and 2 due to SAEs.
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PMC11764090
SiICE1 (Table 2) binds to MYC in the CBF3 promoter, promoting CBF3 expression and cold tolerance in S. lycopersicum .
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PMC10957991
Besides mammary epithelial cells, TRPV4 has been linked to the differentiation of other epithelial cell types.
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PMC9014716
After 48 hours of transfection, total RNA was extracted to detect ABCB1 mRNA.
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PMC10734950
The cells were then washed with 1 ml of PBS and pelleted again by centrifugation at 1000 g for 6 min.
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PMC11470999
After treating B16 and A375 cells with 5 µM PF for 24 h, immunoprecipitation was performed using an anti‐CIP2A antibody.
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PMC5574817
Gel electrophoresis of restriction enzyme digestion products of the plasmid map.
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PMC10530622
An effort should be made to go beyond this and explore the functionality of the microorganism’s community, making use of metagenomics, metatranscriptomics and metaproteomics techniques.
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PMC8526701
The chemosensitization effects of BRD9 inhibition and imatinib are mediated by PUMA through cotargeting the AKT/GSK-3β/NF-κB axis.
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PMC10940855
Importantly, when we cultured human RPMI-8226 multiple myeloma cells harboring shRNA targeting EFNB2 (sh-EFNB2) with BMEC-60 cells, this abrogated BMEC-60–mediated expansion of multiple myeloma cells in 5-day culture, showing that BM EC–mediated expansion of multiple myeloma cells in culture was dependent on EFNB2 signaling (Fig. 2G).
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PMC11286266
To investigate potential modulators of ATF6α activity following the CRISPR/Cas9 high-throughput screen using biochemistry and microscopy-compatible tools, two mammalian cell lines were engineered.
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PMC11045125
GSEA analysis of RNA-seq results confirmed that Myc-expressing tumors have upregulated expression of genes in the “Hummel_Burkitt_Lymphomas_Up” gene set (Fig 4B), which distinguishes genes expressed in human Burkitt lymphomas versus human GC type DLBCLs .
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PMC11575040
In each well, crystal violet was solubilized using 2 mL of 10% acetic acid, and the absorbance (optical density) of the solution was measured using a spectrophotometer at a wavelength of 620 nm.
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PMC10440586
The next three tracks indicate the P value of three transcriptional subtypes.
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PMC6600253
For instance, the results of clinical trials showed that PT is generally safe for use in humans at amounts up to 250 mg/day and is also safe for treating or preventing cardiovascular disease .
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PMC11666785
Depending upon the degree of correlation, the single molecule in the consecutive frames are identified as pair and sorted as shown in the adjoining table (see, Fig. 1 and Supplementary Note 1).
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PMC9388315
In the present study, it was seen that cinnamon extract reduced all three ErbB2, HSF1, and LDHA genes.
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PMC11753435
More than 80% of pathogenic proteins are considered “undruggable” by standard small-molecule inhibitors, as they often lack putative stable binding pockets (1, 2).
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PMC9220170
In addition, it was shown that SELENOT is required for the attachment of glycosylphosphotidylinositol to the C-terminus of proteins during their post-translational modification, which is extremely important for the efficient processing of more than 150 proteins .
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PMC11088135
B qRT-PCR was performed to analyze the mRNA expression of YAP1 in U251 cells.
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PMC11347429
Data files and data sets generated or used in this study and their accessibility in public repositories.
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PMC11650848
A Detailed CpG methylation analysis of the ZFP57 alternative promoter analysed by pyrosequencing in the cord blood.
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PMC9429973
Results: Patient’s 4T’s score was 7 (2 for platelet count fall, 2 for timing, 2 for catheter associated venous thrombosis and 1 for other causes), strongly positive IgG anti-PF4 antibodies (OD: 2.43, cut-off<0.3 units) and a HIMEA profile favoring HIT (typical sigmoid curve at HIMEA low, >50% decrease of AUC at HIMEA high, absence of platelet aggregation at HIMEA saline).
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PMC11538390
The variation in allele frequency and CP prevalence across distinct regions are illustrated by the intensity of the blue shading.
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PMC11365983
Foxc1 shRNA knockdown induced a two-fold reduction in levels of pY754 autophosphorylated PDGFRα (P = 4.5 × 10), while Foxc1 overexpression resulted in a 3 fold reduction (P = 3 × 10; Fig. 5D–F).
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PMC11759751
Student’s t test was used to determine the statistical significance. *
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PMC9429973
Venetoclax therapy was started at the 3rd month of ibrutinib (from the escalation phase).
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PMC6450504
This behavior was not observed on HeLa cells, with enhanced cytotoxicity of bPEI-coated PTX liposomes equivalent to the free drug (Figure 4(a)).
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PMC11462673
G Knockdown of LDB1 increased the Annexin V375 fraction and promoted apoptosis in Jurkat, 6 T-CEM and J.gamma1 cell lines.
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PMC8873393
F Distribution of number of exons for “induced”, “recovered” and “repressed” annotated lncRNAs.
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PMC11467964
STS includes more than 80 histologic subtypes.
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PMC6771295
Protein levels of SIRT1, SIRT3, and SIRT5 were determined by SDS PAGE followed by Western blot.
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PMC11406030
The favorable prognostic impact of nuclear LMTK3 in the univariable analysis persisted; however, cytoplasmic LMTK3 did not exhibit statistical significance.
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PMC11088135
p < 0.01 vs. HEB group Expression levels of YAP1 in glioma cell lines.
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PMC9429973
PET-CT was performed on day 28 and 3rd month to evaluate the response rate.
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PMC9429973
Aims: The aims of this study were to elucidate the predictive associations between mRNA-seq profiles and independently verified genomic lesions, and to develop easy-to-interpret transcriptome-wide biomarkers for ALL subtyping in the clinical setting.
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PMC11367146
Volcano plot distribution for GEO datasets (D) GSE55457 (E) GSE12021 (F) GSE55235.
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PMC10040136
Paired samples from each patient comprised one sample prior to targeted treatment, demonstrating sensitivity to Imatinib, and one sample post-resistance development.
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PMC11359735
C, [H]-ethanolamine labeling.
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PMC9429973
Biallelic ERCC6L2 germline mutations cause BMF and accumulation of somatic TP53 mutations in bone marrow, contributing to the disease progressing into acute myeloid leukemia.
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PMC11365983
FOX genes have fundamental physiological functions, that extend from angiogenesis and organ development, to cell cycle control.
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PMC2196094
Membranes were hybridized with the different probes in 6× SSC, 3× Denhardt's solution, 0.5% SDS, 2 mM EDTA, and 100 μg yeast RNA/ml at 65°C and were washed four times at 65°C during 60 min under high stringency conditions (0.1× SSC, 0.1% SDS).
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PMC10391797
P values were calculated using two-sided one-way ANOVA (for C–D).
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PMC10956161
PRDX6 plays a leading role in maintaining redox homeostasis in mammalian cells by reducing a wide range of peroxides in cells.
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PMC7762347
In comparison to solvent control, we determined increased frequency of cells in G0/G1 phase in CYN (0.25 µg/mL) and CYN/MCLR (at 0.5 µg/mL/1 µg/mL) exposed cells together with reduced number of cells in S phase.
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PMC11470381
Figure 4.Bisecting cell ROI into front and back halves.
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PMC9429973
Of patients with BM fibrosis scoring post-baseline (N=52), 31% improved by 1 grade and 50% were stable.
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PMC11770199
Five days after tumor implantation, the mice were intratumorally injected with 2 × 10 mMSCs-tet-p14 and then randomly assigned into 2 groups.
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PMC6889484
A and B) Replication kinetics of NL4-3 in parental (A) CEM and (B) PM1 cells, with no detectable virus 27 days post-infection in 4 and three independent CEM/D128K and PM1/D128K cell lines, respectively.
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PMC8303372
The need for an HCC therapy based on the selectivity of the molecules towards specific target receptors is evident.
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PMC11686380
Quantification of RFP + cell distribution across the cortex (divided into ten equal bins) is shown.
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PMC5925824
After 5 days in culture, supernatant was collected and an IFNγ ELISA (R&D Systems SIF50) was performed according to manufacturer’s instructions.
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PMC11502327
In order to improve the low water solubility and severe side effects of CPT, Zhou et al. (199) established a safe and effective N-trimethyl chitosan (TMC) coated with the CPT drug delivery system, CPT-TMC.
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PMC10988555
In the past decade, a large number of cobalt complexes have been developed for therapeutic applications, demonstrating its therapeutic potential.
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PMC11680982
This study had limitations.
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PMC11261875
Takafumi Shima: Data curation, Investigation, Writing – original draft.
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PMC11751675
Its water-soluble form is currently being recommended for further pre-clinical and clinical trials due to its potential as a novel antiviral agent.
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PMC6642070
SAMS causes statin intolerance and lack of adherence, which increases 36% of recurrent myocardial infarction (MI) and 43% of coronary heart disease (CHD) events .
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PMC4270159
18–24 hr after seeding treat wells in Cohorts 1–3 with conditions as described in the Sampling section.
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PMC11194678
COS7 cells (RRID: CVCL_0224), SH-SY5Y cells (RRID: CVCL_0019), and U251 cells (RRID: CVCL_0021) were from American Type Culture Collection, grown in Dulbecco’s modified Eagle medium (Hyclone) containing 10% fetal bovine serum (FBS; Hyclone).
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PMC9429973
Multiplex ligation-dependent probe amplification (MLPA) was performed to detect large fragment deletions in patients with negative results in the targeted-seq but conforming to the FA by MMC-induced chromosome breakage experiments test and clinical manifestations.
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PMC10092581
In general, the Au(I) selenourea complexes were shown to be more effective than their Ag(I) counterparts in hampering the selenoenzyme; with [AuCl] being the most effective derivative, and able to almost completely abolish TrxR1 activity even at 0.5 nM (Figure 3, panel A).
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PMC11787355
and analgesia (Carprofen, 5 mg/kg s.c.).
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PMC11424574
Immunohistochemical assay revealed that significantly decreased the Ki-67 and MTA2 expression in MTA2 depletion mice, compared with luciferase knockdown (shLuc) control mice (Figure 6D).
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