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PMC11697189
The results suggested that B cells naive, NK cells resting, Dendritic cells activated, Eosinophils, Dendritic cells resting, Mast Cells resting, Macrophages M1, T cells CD4 memory resting, Monocytes and Macrophages M2 were negatively correlated with risk scores (Fig. 7A), while T cells follicular helper, T cells regulatory (Tregs), T cells CD4 memory activated and Macrophages M0 were positively correlated with risk scores (Fig. 7B).
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PMC11371747
PLX was the most potent drug, possibly because it has a highly efficient RFC uptake and conversion to polyglutamate forms that inhibit both DHFR and TS (Raz et al., 2016).
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PMC11144382
In the forebrain, rostral-most tissues at the gastrula and neurula stages are believed to be devoid of RA signaling due to the action of Cyp26A1 and Cyp26C1.
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PMC11423992
A11013, Invitrogen) or Mouse anti-Human Kappa Light Chain Secondary Antibody, APC (cat. #
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PMC11592837
In a common theory, it is assumed that fragments with a length of <180 bp are formed due to apoptotic nuclease activity .
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PMC9910796
Transfections were performed with lipofectamine 3000 transfection reagent (Thermo) in Opti-Mem (Life Technologies) according to the manufacturer’s indications.
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PMC11665584
Nevertheless, our scRNA-Seq and Xenium data sets allow for more refined interrogation of the cellular interactions underpinning tissue specificity.
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PMC11301242
Subsequently, we downloaded RNA-seq gene expression data of cell lines from the Cancer Cell Line Encyclopedia.
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PMC10748106
The dipole moment of the remaining simple iridoids was higher, ranging from 4.2 to 5.78 debye, compared to Genipin, which exhibited a lower dipole moment of 1.26 debye.
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PMC11761919
p < 0.05, **p < 0.01, ***p < 0.001.Fig 6 Detection of immune protective efficacies against FAdV-4 challenge. (
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PMC11746948
In Fig. 4k, the box plot is presented to illustrate the correlation between the sensitivity of [860 cancer cell lines to 481 compounds] and [different levels of ZDHHC20 gene expression].
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PMC11596967
The cells were then immediately analyzed by flow cytometry, recording at least 10,000 events per sample.
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PMC9429973
Additional survival follow-up may assist in refining the switch point of the hybrid model and confirm presence of LTS.
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PMC10490530
Nuclei collected in CHIP lysis buffer (50 mM HEPES-KOH pH7.5, 140 mM NaCl, 1 mM EDTA pH8, 1% TritonX 100, 0.1% SDS, 0.1% Sodium Deoxycholate, and proteinase inhibitor) were sonicated to obtain an average of 200–400 bp chromatin fragments.
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PMC9429973
The results for each of the classifiers are summarized in Table 1.
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PMC9646302
RNA sequencing data were downloaded from TCGA database (https://portal.gdc.cancer.gov/).
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PMC10900886
Alternatively, it can infect imDCs, which can transfer the virus to lymphoid tissues.
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PMC11802929
The color code of the captured cells is that used in SARS-CoV-2 proteome of panel (a).
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PMC11301095
Autoimmune encephalitis panel comprising six markers and the complete immunoglobulin profile were within normal limits.
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PMC9596868
Values represent mean ± SEM of two independent experiments analysed by ANOVA with Dunnett's Multiple Comparison test. *
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PMC10968586
The detected cytotoxic effect of HT was caused by HT-mediated H2O2 accumulation in cell culture medium, overcoming HL-60 ability to clear ROS .
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PMC7802142
c Cell growth curves of CCK8 assays for DPCs after treatment of PBS, ReN-NV, or 293-NV.
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PMC6835428
Thus, an additive/synergistic effect could have been expected.
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PMC11707832
Intriguingly, our analysis unveiled high expression levels of MAN2B1 in monocytes and DCs, whereas it exhibited lower expression in granulocytes, T cells, B cells and NK cells (Figure 1).
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PMC11714165
In addition, activation of this pathway can also lead to overexpression of the anti‐apoptotic protein Survivin, ultimately inhibiting tumor cell apoptosis.
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PMC8557138
Hydrogel-based perfusable vascularized tissues help in modeling the in vitro processes better and enhances the efficiency of in vivo transplantation.
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PMC11783017
After injection of liposomes, the fluorescence signal in all mice tumor sites gradually increased and reached its maximum at 24 h, while the tumor accumulation in the Lipo group was much lower compared to the Lipo-IL11 group (Fig. 5A and B).
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PMC11348300
This work introduces a fundamentally unique type of functional imaging approach, here termed pressure-enhanced sensing of tissue oxygenation (PRESTO) which is based around the finding that this commonly available human-use contrast agent can be used for stimulating immediate fluorescence when tissue hypoxia is present (Fig. 1).
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PMC11739484
Fig. 6This figure showcases the exploration of mutational profiles and construction of prognostic model of MMP7, MMP11, and MMP14 genes across skin cutaneous melanoma (SKCM) samples using the TCGA cohort.
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PMC9429973
These titers substantially increased post the 3rd vaccine dose.
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PMC11476106
The mounted plates were scanned with Ventana iScanHT (Roche, Basel, Switzerland) at 20× and the resulting images were saved in TIF format.
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PMC9429973
Trial sequential analysis was adopting to examine the power of analyses in primary outcomes.
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PMC11267830
The parameters included trypsin/P cleavage with ≤ 1 missed cleavage, N-terminal Met excision, carbamidomethyl on Cys as a fixed modification, and an FDR < 1%.
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PMC11682525
Cells were exposed to this mixture for 5 minutes and the ZOE fluorescent cell imager was employed to capture images.
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PMC9784246
Unessential residues and water molecules were removed.
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PMC10578720
CS and PDHC enzymatic activities in human THP-1 macrophages following treatment with different HBV titers.
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PMC11743316
For instance, in ovarian malignancies, the total amount of mitochondrial DNA (mtDNA) is much higher than in controls .
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PMC9596868
Our results demonstrate strong synergism between plant- derived flavonoid quercetin and telomerase inhibitor MST-312.
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PMC9429973
Methods: Key eligibility criteria: cytoreduction required, platelet count >450x10/L, haemoglobin (Hb) ≥10 g/dL. The starting dose is 0.6 mg/kg/d and titrated, if needed, to a platelet count of 200-400x10/L. Results: At data cut-off (3Feb’22), 44 patients had enrolled.
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PMC8345486
Compound 23 also had a noticeable cytotoxic effect on the large mature spheroids of A2780 cells, where the ability of compound 23 to penetrate the interior of the spheroid was demonstrated.
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PMC11670954
The fluorescence intensities of the target proteins and DAPI in the selected six regions from each group were quantified using Image J. The specific operation process is as follows: ImageJ.exe →→ File →→ Open →→ Image →→ Color →→ OK →→ Image →→ Color →→ Split Channels (blue (DAPI) and green (ZO-1, Occludin, RhoA, ROCK2, and MLCK), do the following for each picture with two colors separately) →→ Image →→ Adjust →→ Threshold (Use the default threshold) →→ Apply →→ Image →→ Auto Threshold →→ Try all →→ OK→→ Default →→ Threshold →→ Default (If the threshold set by the default algorithm does not meet the requirements, another algorithm is needed.)
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PMC11802855
F Survival curves for progression-free-survival time of patients in different CXCL7 groups.
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PMC7823217
Cell viability after treatment with TMZ.
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PMC11521786
Additionally, these results suggest that the Mv-HER3 vaccine may circumvent tolerance and induce high antibody titers in patients.
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PMC11599565
This study was approved by the Ethics Commission of the Ruhr-University of Bochum, Medical School (register numbers: 4042-11 and 5235-15) and all methods were performed in accordance with their relevant guidelines and regulations.
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PMC9429973
Both pre- and post-blinatumomab MRD levels retained significance in bivariate analysis for DFS Image: Summary/Conclusion: The present study underlines the efficacy of blinatumomab in consolidation after chemotherapy-based salvage, showing comparable outcomes between patients treated in CR2 and CR1.
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PMC11343332
2μg of RNA was used for cDNA synthesis using reverse transcription using a PrimeScript 1st strand cDNA synthesis kit (TakaRa, Japan).
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PMC5810978
Human NoVs (HuNoVs) have been recognized for over four decades.
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PMC11787355
tumor imaging with preclinical MRI.
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PMC11544122
Cy5 labeled HIS-SNX9 [20 nM (d) or 5 nM (e)] was incubated with varying concentrations of carboxyl-terminal (CT) peptides (amino acid residues 321–352).
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PMC5443223
Research has demonstrated that mutations in CEP164 are one cause of nephronophthisis-related ciliopathies (NPHP-RC) (9).
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PMC10291556
Also, interestingly, a series of Pt(IV) prodrugs derived from cisplatin with NSAIDs, naproxen, DCF, and flurbiprofen, in the axial position were shown to exhibit superior antiproliferative activity compared to parental cisplatin as well as an ability to overcome tumor cell line resistance to cisplatin.
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PMC11365983
Experimental design for analysis of differentially expressed genes in the meninges of Foxc1 embryos (4 Foxc1, 5 Foxc1) and mice with severely perturbed Pdgfrα signaling (Pdgfc; Pdgfra). (
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PMC7171147
log rank p < 0.05.
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PMC10565698
Cells transfected for 72 h were collected and lysed in a 100:1 mixture of RIPA buffer and phenylmethylsulfonyl fluoride (PMSF; SolarBio, Beijing, China).
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PMC8592717
PCA prevented apoptosis induced by oxLDL by activating JNK/Nrf2 survival signals in J774A.1 macrophages, an in vitro model for investigating pathological atherosclerosis processes .
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PMC6901583
This involvement of TAMs via CCL2 induced by BRD4 can increase uncovering further mechanisms as well as addressing the treatment of GIST.
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PMC9611901
Nowadays, synthetic tannins are increasingly used and research in this field is growing.
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PMC9428827
Food and water were administered ad libitum.
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PMC11499955
24 hours later, the urine sampling revealed an important release of the TMPP-H24 compared to the TMPP-H33 and TMPP-H6 tags that can be correlated to the higher expression of EGFR in the A431 model.
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PMC11371627
Examinations showed raised creatine kinase (CK) levels, neuropathy and clinical features of myopathy.
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PMC11472569
Over the past decade, at least 13 ABC transporters have been implicated in the development of MDR in vitro.
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PMC7823217
C. (a) Drug dose response of glioblastoma cells in 3D bioprinted models.
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PMC10154881
p = 0.111), rMV anti‐NK1.1 treated (n.s.,
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PMC11343332
This migratory capacity is acquired by cancer cells through alterations in cytoskeletal dynamics and adhesion properties, enabling them to detach from the primary tumor and invade adjacent tissues.
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PMC6102174
Swericinctosides A and 9-epi-swertiamarin also showed moderate activity inhibiting HBsAg secretion with IC50 values of 0.32 and 1.06 mM, and HBV DNA with IC50 values of 0.46 and 0.62 mM, respectively .
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PMC11574271
Fig. 4A).
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PMC8633974
The diameter of luciferase LNP was 73.08 nm and GFP LNP was 76.62nm measured by dynamic light scattering with PDI values of 0.083 and 0.098 respectively (Figure 8b and c).
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PMC11772585
As expected, we detected decreased mRNA stability in the shMETTL1 group (Fig. 5F).
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PMC9429973
A strategy to overcome this interference is to staining PCs with anti-CD38 nanobody JK36.
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PMC11750712
Cell counts were extracted from image cell segmentation and distribution percentages calculated for the following marker combinations: CD4 single; CD4PD-1; CD4FOXP3; CD8 single; CD8PD-1; CD8FOXP3; and CD4CD8.
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PMC9920575
All these events are characteristic of cells entering apoptosis .
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PMC11089031
786-0 is a ccRCC cell line derived from a primary clear cell adenocarcinoma (https://www.atcc.org/products/crl-1932) and Caki-1 derived from a primary clear cell adenocarcinoma with metastasis (https://www.atcc.org/products/htb-46#detailed-product-information).
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PMC11026382
Growth rates were measured in triplicate using a plate-reader-based assay (28 measurements total; Fig. 2c, Supplementary Fig. 1a,c).
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PMC8540611
In terms of the surface dendron charge, this is ~40:1 for AuNP13, 15:1 for AuNP14 and 7.5:1 for AuNP15.
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PMC11194678
Experiments were conducted in a blinded fashion.
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PMC11593031
Furthermore, while previous work has generally relied on aCGH methodologies to elucidate chromosomal changes, our employment of both aCGH and molecular cytogenetic analyses has unveiled more nuanced aspects of karyotypic instability, including the presence of unique amplifications and neocentric derivatives that were previously undocumented.
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PMC9096373
Calculation of CD31-, Ki-67-, and γ-H2AX-positive cells was similar to that used for VEGFR-2.
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PMC10482220
Furthermore, this effect is specific for G3BP because truncation mutants, or RRMs of unrelated proteins, were unable to assemble SGs, although G3BP domain D had a weak effect (Fig. 2 A).
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PMC2614415
On the day of analysis, pellets were washed once with PBS and digested with 500 μl of 0.1 mg/mL PBS/RNaseA (Sigma-Aldrich, St. Louis, MO) by incubating at 37°C for 15 minutes.
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PMC3019555
12 equal-volume (1 ml) fractions were collected with continuous OD254 measurements (Vis Detector UA-6; Isco UV).
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PMC4485648
However, based on the other reports, SFN increases cell death in hypoxic tumor cells, especially at higher doses (13,36).
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PMC11380454
FRET was corrected and calculated as previously described 42.
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PMC11687663
The liquid chromatographic system was UPLC Acquity H class series system where separations were achieved on a WATERS XBridge (50 X 4.6mm 3.5μ), C18 column.
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PMC11066331
In 2015, in our study by Valletta et al. reported that MS combined with artificial neural networks (ANN) can quantitatively estimate cell numbers in binary mixtures of mouse embryonic stem cells and mouse fibroblasts, or mouse and human embryonic stem cells .
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PMC11011837
LC/MS analysis was conducted using an Agilent system consisting of an Agilent 1260 Infinity II liquid chromatograph consisted of a quaternary pump, a thermostatted column compartment, and an autosampler coupled to an Agilent 6530 Accurate-Mass Q-TOF (Quadrupole-Time-of-Flight) analyzer and an Agilent JetStream source.
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PMC7229095
100× hypoxanthine and thymidine (HT) supplement (Thermo Fisher Scientific, Waltham, MA, US) was added at 2× in each media to grow the host cells, and 6 mM of l-Glutamine (Thermo Fisher Scientific, Waltham, MA, US) was added as well.
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PMC9611901
As far as we know, the ban happened in 2021, while our research was conducted mostly prior, or parallel to that ban.
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PMC11633763
Representative images of EdU assays of 143B cells stably transfected with shUSP22 in the presence or absence of p‐HK2. (
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PMC4005030
It influences phases 1 and 2 of the metabolism of certain carcinogens and, perhaps, directly blocks specific binding sites of ultimate carcinogens with DNA molecule, thus preventing adduct formation that may result in mutations and neoplastic transformation.
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PMC11484188
When the cells reached 80% confluence, they were digested at 37˚C for 2 min with 0.25% trypsin and passaged onto a 6-well plate at a density of 2x10 cells/well.
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PMC9780037
These delivery systems are composed of biocompatible materials, which are effective in avoiding immune reactions of the host.
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PMC9429973
The average age was 72.44 ± 12.53 with a Body Mass Index (BMI) of 27.94 ± 5.17.
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PMC11693599
The reduced number of EVs in 3D-cultured cancer spheroids appeared to differ under different cell culture conditions.
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PMC11473843
Graphical data are mean ± SD.
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PMC8097906
Transcript expression analysis of RH-30 cells after 48 h VSM treatment (1, 10, 25, 50 and 100 μg/ml) by RT-PCR of ß-actin and GAPDH (loading control); pax3-foxo1 (RMA marker); GLDC, AMT, GCSH and DLD (components of the glycine cleavage system); SGPL1, SPHK1 and SPHK2 (sphingolipid metabolism); as well as ezrin, CXCR4, P-cadherin, syndecan-4, prominin and β-catenin (metastasis and tumor signaling marker). (
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PMC11697065
Since our multi-omics analysis of clones failed to identify relationships among high qP expressing clones, we hypothesised that the high secretion phenotype might be dominated by stochastic properties of different clones.
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PMC11686380
Electroporation of pSuper-mGAP43#1 or #2 strongly suppressed dendritic arborization (Fig. 4A).
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PMC11476222
We believe these criteria provide a sound foundation for data processing.
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PMC11269933
Membranes were blocked and incubated with primary antibodies and secondary antibodies using eZwest Lite Automated Western Device (GenScript Biotech, China).
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