PMCID
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PMC9920575
We evaluated the effect of S. chinantlense extract on a human cervical cancer cell line, HeLa, and a nontumorigenic epithelial cell line, HaCaT, using a crystal violet assay.
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PMC9429973
Treatment with 9.4 mg/kg imetelstat resulted in 32.2% symptom response (total symptom score [TSS] reduction ≥50%) at Week 24 and median overall survival (OS) of 29.9 months with overall study follow up of 27.4 months.
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PMC10854447
Found: C, 65.08; H, 4.67; N, 21.28.
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PMC11507371
However, cancer cells exceed the apoptotic threshold faster when exposed to additional ROS stress than non-malignant cells, which have a “spare” antioxidant capacity.
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PMC9693627
Pentatricopeptide-containing proteins (PPR proteins) are good candidates for the role of the regulators of mRNA translation in human mitochondria.
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PMC9429973
Real time PCR was used to examine the mRNA changes of CD47.
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PMC11519567
Data are mean±s.d.,
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PMC9429973
The patients did not show any differences between first and second sample.
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PMC11802929
These properties, including charge, hydrophobicity, weight, bulkiness, polarity, sulfur presence, and aromaticity, were calculated based on Supplementary Data 3 (Amino acid property scales used for peptide and TCR residues).
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PMC10743717
Its acetylation regulates its enzymatic activities and the formation of mitochondrial RNase P .
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PMC9100622
The data were deposited at the Gene Expression Omnibus (GSE 132966, GSE 156653, GSE 150777, and GSE 164373).
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PMC10976516
OV therapy can only reach sufficient clinical efficacy if the problem of targeting most if not all metastases has been addressed and that is why there is an urgent need to optimize systemic (intravenous) OV application against lung cancer.
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PMC11525028
Representative IHC images showing the expression of NAT10, ATF4, ASNS, and Ki-67 in the mouse model.
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PMC10777969
From the point of view of the suitability of these compounds as potential anticancer drugs, FCP26 seems to be better than FCP23.
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PMC2614415
Through microarray profiling of ovarian cancer samples, we have observed that Aurora-A was significantly overexpressed in ovarian carcinomas compared to adenomas.
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PMC10698968
CPD were calculated at each passage as log2(N1/N0), where N0 is the number of cells seeded and N1 is the number of cells harvested at the end of the passage.
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PMC9429973
Improvements in dyslipidemia, liver function, liver sphingomyelin clearance, and plasma lyso-sphingomyelin in crossover patients paralleled those seen in the original olipudase alfa patients in Year 1; original olipudase alfa patients maintained these benefits in Year 2.
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PMC11618052
0.58 (hexane : ethyl acetate, 1 : 2, v/v).
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PMC11572213
In addition, when the GLUL gene was knocked out in the HL-60 cell line, it was found that GLUL-knockout cells were shown to have decreased cell viability in both glucose- and glutamine-deficient media.
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PMC11476264
For immunoprecipitation assays, indicated plasmids were co-transfected with HEK293T cells in six-well plates.
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PMC11746948
Statistical analyses: two-way ANOVA with Tukey’s multiple comparisons test for (c, e–g, i); one-way ANOVA with Tukey’s multiple comparisons test for (a, b, k, l, n).
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PMC11543885
CRITICAL: The medium must be warmed up prior to the addition of phenol-biotin to prevent precipitation.
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PMC11766309
In the next step, we calculated the correlation between YKT6 gene expression and copy number in each tumor via copy number data from the cBioPortal database (Figure 6B).
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PMC10094712
After the addition of hSELENOM to the medium, a decrease in the baseline level of Fura-2 fluorescence by 50% was observed, and the TG-induced signal was reduced by 70%, relative to the control (Figure 8B).
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PMC11365427
Image created with BioRender.com with permission). (
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PMC11476837
Then, cells were resuspended in 100 µL of 1× Binding Buffer and transferred to cytometry tubes.
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PMC9848491
IC50 of SB.216763 in high and low risk groups. (
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PMC11718809
Four DECs were randomly selected for validation by qRT-PCR.
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PMC11438317
Then, cells were fixed and stained with specific antibodies for SLP76.
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PMC11243198
To induce Hh pathway activity, C3H10T1/2 cells were treated with SAG at its EC50 concentration (30 nM) (Supplemental Figure S1A) followed by GANT61 analog compounds added in dose–response.
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PMC11786767
Representative images of Crtl-miR- and Ki67-miR-expressing cells are shown.
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PMC11476264
The upregulation of E-cadherin is indicative of EMT and is expected to decrease cell motility and invasion.
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PMC11578343
A “Low‐to‐High” frequency device (pink) introduced cells to a low frequency in zone one and then to increasing frequencies in the subsequent zones (from L p = 500 to 125 µm).
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PMC11683240
Cells were left untreated as control (Con) and the experiment was performed as described in (b-d).
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PMC11757230
Comparison of mean tumor mass weight on day 22 in xenotransplantation mouse models treated with different doses of cADSCs. (
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PMC9429973
Median of age was 66.5 years (range 40-82) and 71.5 years (range 53-85) for GCB and ABC, respectively.
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PMC11337594
Cells were treated with different doses (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 µg/ml) of cisplatin or TER, and then assessed by MTT assay (n = 4). (
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PMC11489351
However, for many pH-sensitive behaviors, such as cell cycle progression (2, 3), epithelial-to-mesenchymal transition (EMT) (17), metabolic regulation (18, 19), and differentiation (4) the mediating pH sensors are unknown or uncharacterized (11).
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PMC11286266
Samples were harvested and lysed in the presence of 20 mM N-ethylmaleimide (NEM), immunoprecipitated (IP) using GFP-Trap Agarose (ChromoTek) and analysed by immunoblot (IB) using an anti-FLAG M2 antibody to detect ATF6α.
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PMC9429973
We evaluate the rates of remission, death and overall survival (Kaplan-Meier method).
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PMC11626627
We constructed overexpression and knockdown vectors for circATP2B1 and TWIST1 (oe-circATP2B1/sh-circATP2B#1/ sh-circATP2B#2, oe-TWIST1/sh-TWIST1#1/ sh-TWIST1#2).
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PMC10656496
Coculture Panobinostat and trametinib treatment.
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PMC11513011
DIB is a new name more consistent with chemical naming conventions, as in our previous study.
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PMC4443654
Thus it is unclear whether such results are relevant to other cell types and species, or whether the inclusion of a broader range of features would provide additional insights.
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PMC10197932
To confirm of 2T treatment over all leukemia cells (myeloid and lymphoblastic leukemia cell lines) we also performed a US treatment and proliferation assay on Jurkat cells and healthy lymphocytes.
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PMC6268318
The plant C. erythrophyllum (Burch.)
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PMC6337547
The source pathways of other phenolic acids, such as salvianolic acid B downstream of rosmarinic acid, have not yet been elucidated.
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PMC11703992
An obvious increase of DEGs was observed in both GCA and old groups, which might indicate gene expression variation of CD4+ Memory T cells was quite similar in GCA patients and elderly.
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PMC9429973
Clinical attention and action should be directed upon this matter.
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PMC9429973
Results: As of 8 February 2022, we enrolled 23 patients: 11 low grade NHL (LG-NHL:7 with FL and 3 with MCL), 7 DLBCL and 5 CLL.
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PMC11683240
The recombinant proteins Gαi and PTS1 were expressed and purified as described earlier (62).
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PMC8658661
Since then, HepG2 cells have been entered into the ATCC (American Type Culture Collection, Rockville, MD, USA) repository as a human cell line (HB 8065), “derived from the liver tissue of a 15-year-old white male with a well-differentiated hepatocellular carcinoma” .
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PMC11648299
Seven patients had been prescribed oral acitretin with daily doses ranging from 10 to 40 mg.
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PMC9429973
Aims: The aim is to assess the clinicopathological characteristics survival and treatment in Richter transformation patients diagnosed and treated in Hematology Clinic of Craiova in the last 10 years.
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PMC11401358
The desalted silk fibroin solution was collected and centrifuged at 9800 G/min for 20 min at 4 °C to remove silk aggregates .
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PMC9398137
In contrast, only the NOXA protein was detected in MCL1 complexes in S63845-resistant DLBCL cell line RIVA (Fig. 3B).
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PMC9429973
The accepted minimal number of stem cells that will provide the reconstruction of hematopoiesis after high-dose chemotherapy is >2x106/kg CD34(+), and the optimal number is >5x106/kg CD34(+) stem cells.
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PMC11012313
Quality control (QC) was performed daily prior to use and included adherence to specific criteria based against a specific lot of QC Fluorospheres (Beckman Coulter, Indianapolis, IN, USA) including a gain difference of less than or equal to 20% from the target gain, the median fluorescence intensity (MFI) difference less than or equal to 5% from the target MFI, as well as a robust coefficient of variation (rCV) value less than or equal to 5%.
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PMC9429973
Aims: To evaluate MRD as a prognostic marker for DLIs after alloHCT.
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PMC9739791
Almost all NCs 12 were bound to erythrocytes, while the binding efficiency of NCs 9 was only 1%, indicating that NCs 12 efficiently bind with erythrocytes via an ERY1 peptide linkage.
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PMC11532793
If bootstrapping is used instead of Gibbs, then optimal results are already achieved with N(B − 1) about 50, with little further gain from drawing further technical samples.
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PMC11588008
Oxidative stress is an important factor that interrupts normal cell proliferation and apoptosis .
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PMC10957991
As Slug protein has been thought to act as the main determinator of the lineage specific differentiation of the mammary epithelial cell populations, we verified the level of Slug in both 184A1 and MCF10A cells, depleted for TRPV4 by siRNAs or CRISPR, respectively.
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PMC9429973
Noteworthy, the suspension of ghosts induced a similar activation on HUVECs compared to whole hemolysate suggesting the major role of RBC membrane-derived particles in early hemolysis-induced endothelial damages.
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PMC9429973
In 61.4% out of the 83 episodes of R/R disease in these 43 patients, a biopsy was not performed: 74.5% of refractory DLBCL episodes versus 35.7% of relapsed DLBCL episodes (p=0.001).
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PMC11789597
B–D) B‐NDG mice (n = 6) were subcutaneously inoculated with MM1S cells expressing shNC or shCAV1 in the left or right flank, respectively. (
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PMC11803149
For analysis of intracellular molecules, cells were subjected to antibody staining using a Foxp3 staining buffer set (eBioscience, 005253-00), according to manufacturer instructions.
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PMC9429973
Summary/Conclusion: There are various pathogenic microorganisms that could cause bloodstream infection in patients undergoing hematopoietic stem cell transplantation, with high multi-drug resistance rate.
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PMC11754094
P < 0.0001 for TRAC target, P = 0.0007 for PD1 target.
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PMC11424574
The MTA2 knockdown and uPA-overexpressing OS cells (4 × 10/mL) were seeded into the upper part of the Boyden chamber (Neuro Probe, Cabin John, MD, USA).
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PMC11564322
The c-SRC inhibitor I decreased the ERK 1/2 kinase protein expression by 38.33%, compared to the control (Fig. 7A).
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PMC11697703
The authors declare no competing interests.
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PMC11765879
It is characterized by the increased expression of the HER2 receptor, which contributes substantially to the aggressiveness of breast cancer.
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PMC11519567
Binding to the H2AK119ub was shown by immunoblotting.
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PMC11711871
In contrast, 10-week-old GR rats exhibited increased levels of the plasma metabolites 19(20)-EPDPE and 19(20)-DiHDPA on HSD compared to wild type (Fig. 4C).
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PMC11785489
Prenatal TCR sequences from dataset 6 allowed us to further extend our age-related line of inquiry.
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PMC9910796
Cells and purified virus samples were dissociated in Laemmli buffer, heated at 90°C for 5 min and electrophoresed on 12% SDS-polyacrylamide gels.
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PMC9429973
Moreover, the expression of ICD related HSP70/90 and MHC-I proteins, as well as the expression of NLRP3 and caspase-1 proteins were explored.
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PMC11257203
R. Alipour Klour and A. Gholami Shekarsaraye contributed to the experimental studies and data acquisition.
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PMC11711347
Breast Cancer Cell (MCF-7) Cytotoxicity Test Cells were seeded into a 96-well plate and incubated at 37°C with 5% CO2 until a confluency of approximately 70% was achieved.
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PMC11759751
Therefore, CD19 CAR T cells still face a very big challenge.
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PMC10854447
Such a rigid conformation resulted in tight fitting within the EGFR active site with derivatives (R = H, 4-OMe, 3-Cl, 3-Br, 2,4-di-Cl, and 3,4,5-tri-OMe) as the cyclized structure better fits within the bioactive conformation.
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PMC10170482
Accumulating evidence suggests that tumor cells face variable mechanical stimuli that may induce metabolic rewiring of tumor cells.
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PMC11787355
In these engineered primary glioblastoma cells, we found that a reduction of mean PTPRZ1 expression of approximately 60% abolished TCR-T cytotoxicity (Fig. 5j).
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PMC9164404
Ex vivo imaging showed that Abplatin mainly accumulated in the liver and tumor regions (Fig. 3C).
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PMC9429973
Aims: We aimed to investigate EZH2i in a characterized panel of Peripheral T-Cell Lymphoma (PTCL) and T-cell leukemia cell lines in combinational therapy with widely used salvage chemotherapeutics.
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PMC9429973
At the end of cycle 2, the PET-negative rate (Deauville score 1, 2, or 3) was 90%.
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PMC11411004
a CCK8 assay to detect the proliferative growth status of CHO-ADM monoclonal antibody cells treated with different concentrations of I-4 in culture for six days.
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PMC3931643
One set of important TORC1 substrates are the eIF4E-binding proteins (4EBPs), 4EBP1 and 4EBP2 .
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PMC11470999
As such, there is an urgent need for new and effective anticancer drugs that can rapidly translate to clinical use, especially those capable of crossing the BBB.
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PMC9429973
Protocol followed was the COOPRALL 1997 in 12 cases (26%), COOPRALL2007 in 28 cases (61%) and EORTC VHR in 6 cases (13%).
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PMC10253553
Protein lysate (5 to 15 μg) was diluted in 2x laemmli sample buffer with β-mercaptoethanol, heat denatured (~100 °C for 5–8 min), separated by SDS/PAGE, and transferred to immune-blot polyvinylidene difluoride (Bio-Rad, 1620177).
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PMC9559528
HIV-induced CD4+ T-cell destruction hinders this response and may lead to asymptomatic primary infection.
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PMC11486946
Right, non-EGFR-expressing cell lines: CHO-K1 (gray) and Ba/F3 (orange).
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PMC7192625
We first plotted ChAs of each histone mark on the PP subnetwork against each mark’s abundance on the same subnetwork (Figure 3A), also showing the ChAs resulting from distance-preserving randomizations (see Materials and Methods).
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PMC11095939
The Dynabeads were washed with low/high salt solutions and eluted twice in TE buffer containing 1% SDS at 65°C for 15 min.
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PMC3019555
This result was confirmed by transient KD of Ago2 using Ago2-specific siRNAs (unpublished data).
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PMC11387401
All detected interactions, as well as the count tables of all nRIPseq experiments, can be found as supplemental datasets (Datasets EV1, EV2).
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PMC11802929
Created in BioRender.
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PMC11519583
Furthermore, it should be noted that Ror2 and Frizzled-family receptors, another type of Wnt receptors, associate via their extracellular CRDs .
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