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OTAR3088
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PMC11087055
Embryos were then fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences)/PBS for 30 min at room temperature (RT).
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PMC9712534
In the case of TOP2B, its nuclear behavior readily alters in response to various cues, such as reduced ATP levels, DNA damage, and TOP2 inhibitors, suggesting that the nuclear behavior of TOP2B reflects, at least in part, the status of TOP2B in living cells.
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PMC11765678
Our observations suggest that NO or related species may facilitate ER cytotoxicity in the resistant cells.
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PMC11590870
According to what is reported in Table 1, there was a significant difference between the antioxidant activity of MOP and MOPH, which increased by 57% after the hydrolyzing process.
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PMC11499381
A Holm–Šidák analysis was chosen for post-hoc analyses because this analysis technique has high power to detect differences in cases where group sizes are relatively small.
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PMC9841531
The respective substituted aromatic amine (1.1 equiv) was added at 0 °C, and the mixture was stirred for 6 h, followed by stirring for 30 min at room temperature.
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PMC10907726
The apoptosis rates of OS cells were examined by flow cytometry after the treatment of DATS for 24 h. *p < 0.05 (compared with NC). (
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PMC11711871
Both GR rat models presented with normal blood pressure under a standard diet and changed to salt-sensitive hypertension upon high salt exposure, although the endocrine profiles were quite different.
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PMC11705067
Taken together, FDX1 and DLAT are promising targets for the prevention of ccRCC, and cuproptosis may be a novel and attractive strategy for therapy of ccRCC.
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PMC10887351
β-ARs are classified into three subtypes: β1-ARs, β2-ARs, and β3-ARs.
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PMC11190538
Fig. 2 presents the chromatogram output of GPC of the tested C-phycocyanin.
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PMC11060216
As shown in Figure 1 and Table 1, there was no statistical significance among the three groups.
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PMC11806818
Adapted with permission from Long et al. designed a colorimetric aptasensor for breast cancer exosome detection based on iron-copper oxide-copper ion nanozymes (Fe3O4-Cu-NZs) and CD63 aptamers.
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PMC11295144
The normal (Gaussian) distribution of the data was first tested using the Shapiro–Wilk normality test.
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PMC11806613
Right: Glutathione peroxidase (GPX) levels were measured by a GPX activity assay.
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PMC11546117
The differentiation medium used MECM, where BPE was replaced by 10% of FBS.
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PMC4395774
Defects in perforin lead to the failure of T and NK cell cytotoxicity, hypercytokinemia, and the immune dysregulatory condition known as familial hemophagocytic lymphohistiocytosis (FHL).
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PMC2193049
After a further 12-h incubation at 37°C, the culture medium was replaced with 1 ml DMEM lacking methionine and cysteine (ICN Biomedicals, Inc.), and the cells were incubated for 1 h at 37°C.
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PMC9429973
Analysis of MSCs from non-CHIP (n=5) and CHIP (n=5) individuals revealed no difference in surface marker expression over 10 passages.
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PMC9878562
Lymph nodes were fixed for 5h in a solution 4% paraformaldehyde and Vibratome sections were stained overnight with anti-hCD19 Ab (mAb HIB19), anti-mPDPN (mAb 8.1.1).
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PMC11653533
Again, it is an evergreen bush with whitish-grey bark; slender and, more often than not, leaning trunk; spherical to rounded fruits with a width of 1.0––1.5 cm and colored yellow to orange-red (Bunawan et al., 2014; 2014.,
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PMC11011837
These molecules could be the subject of further studies to identify potential biomarkers.
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PMC9388315
The Hochest staining 33285 (Sigma, USA) was used to evaluate the occurrence of apoptosis.
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PMC11746942
Psoriasis is characterized by hyperproliferation, abnormal differentiation of keratinocytes, and infiltration of multiple inflammatory cells .
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PMC7840816
However, a few PTC carriers develop recurrences and distant metastasis (2,3).
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PMC11269933
The phosphorylation of conserved Ser11 in CgPK enhances its enzyme activity (A) The phosphorylation levels of Ser11 site of CgPK in gill tissues of C. gigas and C. angulata during heat stress, which was obtained from our previous study.
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PMC11549612
Real-time quantitative polymerase chain reaction-based expression profiling of BOLA1, BOLA2, and BOLA3 genes in KIRC and control cell lines.
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PMC11574271
Consequently, the activated JNK pathway increased MMP12 expression, ultimately driving bladder cancer cell migration and invasion.
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PMC11777207
Therefore, the notion that high expression of both PD-L1 and CD80 could drive activated DCs to the dLN to prime naïve T cells further emphasizes how similar proportions of PD-L1 and CD80 can result in stimulation rather than inhibition of adaptive immunity.
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PMC9429973
Results: JAK2V617F vav-inductible-Tg/TP53 KO mice were generated to recombine JAK2V617F at an endogenous level in TP53 inactivated hematopoietic cells.
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PMC11321678
If it was above 1, this region was considered to be a candidate for amplification.
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PMC9368503
Synovial sarcoma is a rare cancer type with poor outcomes in the advanced setting despite multidisciplinary clinical management.
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PMC10988555
The best way to evaluate the effect of the complexes in the tested cell lines is by calculating their IC50 (concentration necessary to inhibit 50% of the cell viability) for each cell line (Table 1).
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PMC10667689
Glucose consumption (A, B) and lactate release (C, D) were increased in post-Gal-9 groups compared to the control and PMA group, and this increase is more significant over time in the HL-60 and THP-1 cell line (*p < 0.05, **p < 0.01, ***p < 0.001).
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PMC10729469
CT scans were acquired using TriFoil imaging Triumph (Tri-foil imaging Northridge, CA).
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PMC10669128
An example is the xenograft model investigated by Floris and colleagues, obtained from the IM-sensitive GIST882 cell line.
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PMC10852540
Among these changes, the decrease in EGFR gene expression in RA, DOX, RA + DOX groups was found to be statistically significant (p < 0.05).
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PMC11033180
Statistical significance thresholds were set as follows: “ns” (non-significant) for p-value >0.05, ∗ for p-value <0.05, ∗∗ for p-value <0.01, and ∗∗∗ for p < 0.001.
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PMC11621493
Constructs were transformed into chemically competent Escherichia coli.
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PMC9429973
It is unknown whether pts who achieve similar levels of MRD clearance experience comparable outcomes irrespective of treatment intensity.
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PMC11790971
Three replicates were created for each monoclonal and artificial cell line pools across passages.
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PMC11740573
The tested bacterial isolates presented different profiles of reaction to antibiotics.
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PMC11348300
The images were acquired using a single-photon sensitive intensified CMOS camera (C-Dose, DoseOptics LLC, NH) synchronized with a 50-mW average output power, 635 nm modulated laser diode (BWT, China), used with 20 µs pulses at repetition rate of 500 Hz.
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PMC10092581
IC50 values were calculated by a four‐parameter logistic model 4‐PL (p<0.05).
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PMC8068766
Generally, cells respond to stress in a variety of ways, from activation of pathways that promote survival to initiating programmed cell death to eliminate damaged cells (Fulda et al., 2010).
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PMC11757230
Intestinal lymphoma may be latent in some dogs with chronic inflammatory enteropathy.
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PMC11323699
ovarian carcinoma cells injected either subcutaneously (SC) or intraperitoneally (IP).
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PMC11769522
CPEKs incubated with 60 and 90 ng/mL of the IFN-γ/TNF-α mixture showed significantly increased IL-6 concentrations in their culture media compared to those incubated with 10 ng/mL of IFN-γ (Figure 2A).
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PMC11040965
The non-commercial mAb (provided by S. Ferrone; Massachusetts General Hospital, Harvard Medical School, Boston, USA) whose production and characterization have already been reported , were used as described previously .
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PMC9429973
These genetic alterations induce constitutive JAK/STAT and other kinase signaling that may be targetable by the JAK1/2 inhibitor ruxolitinib.
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PMC9024365
Furthermore, the C-terminal portion of SD4, which consists primarily of membrane associated regions, is sufficient for clustering with GluA1- and GluA2-containing AMPARs, and that clustering of AMPARs is dependent on co-localization with SD4.
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PMC11271800
It has been shown that low imatinib concentrations induce the secretion of the anti-inflammatory cytokine IL-10 by M1 TAMs.
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PMC11543885
To assess the enrichment specificity for NLRP3 proximal proteins, generate a list of experimentally validated NLRP3-interacting or -co-localizing proteins via BioGRID database or literature curation.
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PMC8009078
From the molecular docking of A5 into the active site of the receptor (A5 of Figs. 3 and 4), it was found that an aliphatic chain (4-methylpent-3-en-1-yl) form HB interacted with Gln51 and Lys449 with a distance of 3.37 and 3.90 Å, respectively.
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PMC11185260
In contrast, scL-vec treatment resulted in no demonstrable survival benefit.
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PMC9780037
Serum cyt c as a biomarker for cancer prognosis. (
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PMC9436459
Additionally, these compounds were also active against hypoxia-induced vascular endothelial growth factor (VEGF) secretion in Hep3B cells (Table 3) .
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PMC11342785
All live cell imaging was performed using 60X oil immersion objective (NA 1.4) on a Nikon Ti-Eclipse with a temperature stage at 37 °C and supplied with 5% CO2.
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PMC11476004
In the present study, the SCE assay revealed that the level of damage in sick dogs was three times that noted in healthy dogs.
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PMC11285179
Patients with incomplete data were excluded to evaluate the clinical effect.
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PMC11775197
Experiments conducted by Gao et al. using human SW480, HCT-116, and other cells suggested that PPARα, an E3 ligase, induces Bcl2 ubiquitination and degradation, leading to apoptosis .
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PMC10932641
On the other hand, compared with those of the negative control group, the tumor volume and weight of mice of the oeGPRC5D-AS1 group showed a significant decrease (P<0.05) (Figures 10-12).
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PMC11397654
Some derivatives were found to be active against all five cancer cell lines (MTT assay).
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PMC10976516
Due to viral clearance, normal cells are unaffected.
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PMC11459296
These models facilitate the study of molecular and cellular pathogenic mechanisms and enable the evaluation of the efficacy and toxicity of immunotherapies, thereby accelerating their preclinical and clinical development.
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PMC10956161
These current findings suggest that PRDX6 could not be used as a clinical diagnostic indicator for lung adenocarcinoma patients.
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PMC10040136
Data are means ± SEM *p < 0.05; **p < 0.01 Studies from different research have shown that the expression of the common MSC marker (CD73 or CD90) is also upregulated in gastric cancer cells .
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PMC11459296
All experiments were replicated using at least three CB samples from different donors.
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PMC11795610
Therefore, we examined the expression of cell cycle-related genes by qRT-PCR.
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PMC11714165
On day 10, following the initial treatment, the blood samples were taken and subjected to various analyses.
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PMC3019555
Specifically, 80% of untreated cells contained three or less P-bodies, whereas >70% of cells contained more than three P-bodies upon 2ME2 or TX treatment (Fig. 4 C).
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PMC9429973
Nevertheless, the role of CAR circulating cell-free DNA (ccfDNA) dynamics during CAR-T cell treatment is not clear.
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PMC9429973
Methods: This is a single center retrospective study carried out in National Center for Cancer Care and Research, Hamad Medical Corporation over the period 2008-2021.
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PMC11555465
The results indicated that the culture supernatant of T. gondii tachyzoites altered the morphology of bladder cancer 5637 cells, inhibited cell proliferation, reduced cell migration and invasion and promoted apoptosis.
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PMC11767725
Conversely, commensal bacteria like Cutibacterium and Corynebacterium are diminished, leading to weakened immune regulation.
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PMC9429973
Then we tried to uncover the synergistic mechanism of brequinar and venetoclax.
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PMC11697189
The left semicircle indicates different HSP families with red, black, yellow and gray, respectively.
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PMC9712534
Cells were treated with either DMSO, 100 µM etoposide, or 20 µM ICRF-187 for 2 h. TOP2B and TOP2A were analyzed by Western blotting.
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PMC11345828
In contrast, the ether substituent at the indole 5-position is directed parallel to the shelf region of the protein, which offers significant space and an ideal vector for further exploration.
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PMC8584319
Thapsigargin (Tg), puromycin dihydrochloride, p38 inhibitor (SB203580), and JNK inhibitor (SP600125) were purchased from Sigma (St. Louis, MO, USA).
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PMC2193049
Proteasome was purified from human outdated blood by affinity chromatography using the monoclonal antibody MCP 21 (European Collection of Animal Cell Cultures) immobilized on CnBr-activated Sepharose (6 mg antibody/mg Sepharose; 24).
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PMC8913139
Meanwhile, upregulation of ROCK1 attenuated the inhibitory effect of miR-448 on A-172MG cell migration and invasion (Figures 5(d) and 5(e)).
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PMC11661040
The findings from three independent experiments were succinctly depicted using histograms. (
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PMC5839394
The Ag release from subcellular components was measured by scanning electrochemical microscopy and anodic stripping voltammetry (ASV) using a CHI920B workstation (CH Instruments), as already described .
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PMC11455164
This migratory behavior was significantly enhanced upon treatment with T3 or T4.
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PMC11755519
EV, extracellular vesicles.
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PMC11670407
qPCR results revealed that neither the knockdown nor the overexpression of OTULIN affected the transcription level of GPX4 (Fig. 5b, c).
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PMC11697703
This highlights the robustness and importance of using multiple approaches, each with advantages and limitations.
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PMC11335267
hl, hind limb; t, tibia; r, rectum; sp, spine.
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PMC11726183
TOF HRMS (m/z): [M + H], calcd: 386.1504, found: 386.1504. %
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PMC9024365
Cells were plated at a density of 300,000 cells per well and cultured for 24 h prior to transfection.
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PMC9429973
Differences were considered significant at p<0.05 using an unpaired Student’s t-test.
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PMC9429973
Aims: To determine the ‘real-world’ treatment patterns and treatment pathway of DLBCL patients treated at the second line, with a focus on patients who are ‘fast progressors’ as defined below.
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PMC11093197
The E‐cadherin–β‐catenin complex specifically localizes to the cell membrane and plays a crucial role in maintaining epithelial integrity.
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PMC11680562
Characterization studies, including FTIR, viscosity, spreadability, pH, and stability assessments, confirmed the gel's suitability for vaginal delivery.
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PMC11626627
Fig 3 The circATP2B1/miR-204–3p/TWIST1 axis is involved in the regulation of EMT in ccRCC. (
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PMC8524093
Chikungunya virus have recently become an urgent problem to public health because of several reasons, such as (i) the large and important outbreaks occurred in the last decade resulting in thousands of hospital interventions and hundreds of deaths, (ii) its possible widespread all over the globe as the result of virus ability to propagate in different vector species, and (iii) the high rate of long-term debilitating arthralgia with direct and significant social and economic impact.
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PMC11806818
The H-rGO-Pt@Pd NPs were able to oxidize the colorless TMB to blue oxTMB in the presence of H2O2, which changed the absorbance of the system at 652 nm, and realized the sensitive colorimetric detection of GPC3.
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PMC11622247
Binding of the target induces a conformational rearrangement which increases the distance between the two chromophores and thereby minimizes energy transfer.
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PMC9895440
PT.58.39997829), NR4A2 (Hs.
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