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OTAR3088
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PMC11683240
α1AT, α1-antitrypsin; Gαi, α-subunit of inhibitory G protein; PT, pertussis toxin.
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PMC11628309
Additionally, the effects of MG-132, a reversible inhibitor of the 26S proteasome, have also been examined.
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PMC10253973
The QM/MM approach provided information about the stability of the adducts, showing the highest stability in the case of the complex (72) and the d(ATATAT)2 sequence.
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PMC11637716
Furthermore, SBDS, NMD3, eIF6 and DNAJC21, which are critical factors involved in the final steps of the ribosomal maturation process, were similarly expressed in cells from the patient and healthy donors (Figure S3).
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PMC11066331
Data stabilization was performed to reduce the technical variability and unwanted signal bias as an inherent intra-experimental quality control.
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PMC6442998
Images are representative for three independent experiments and presented as mean value ± SEM.
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PMC11055443
Morphological evaluation of secretome treated Caco-2 cells.
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PMC9429973
The overall goal was to develop novel therapy combinations targeting molecular vulnerabilities associated with advanced and/or PI-resistant MM.
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PMC9325715
The data indicate, that iron rather interacts with bLTF through carboxy groups.
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PMC11750696
Yet, contemporary research has noted that mice lacking CHOP display unaltered Th17 cell maturation (50, 51).
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PMC11746948
Moreover, APT2 knockdown led to an elevation in GPX4 protein levels, while the levels of other ferroptosis-related proteins like FSP1, SLC7A11, and ACSL4 remained unchanged (Fig. 5e).
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PMC11388384
Using this design, we found that cell–cell arrangement did not affect diffusion under the classic hindered diffusion model.
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PMC11467800
Defined TKI resistance mechanisms are far from covering all cases of TKI‐unresponsive CML patients and in many cases the cause of resistance remains unknown.
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PMC11754291
However, the reduction in metabolic activity at the highest blue light intensities points to a trade-off between effective α-MSH induction and cytotoxicity.
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PMC8751435
The data represent the mean ± SD of six replicates and three independent experiments. *
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PMC9429973
At BTKi initiation, significantly more patients in zanubrutinib group (18%) had history of atrial fibrillation than ibrutinib (1%) or acalabrutinib (5%).
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PMC11635519
We fused all of the peptides with the GPI-AP sequence to display each molecule on the cell surface.
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PMC4839679
n = 3 independent biological replicate experiments.
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PMC11412721
Log-rank (Mantel–Cox) tests were used to analyze Kaplan–Meier tumor-free survival curves.
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PMC11537649
Melanoma is a malignant tumor that evolves from nevi or pigmented patches, characterized by high malignancy, easy metastasis, poor prognosis, and high mortality.
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PMC11127909
In addition, a recent study confirmed that EFhd1 could regulate the morphology and energy metabolism of dorsal root ganglion neuron cell mitochondria .
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PMC10667689
In the same context, we observed a significant elevation in the concentration of intracellular GSH.
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PMC10713411
Seventeen genes that were highly differentially expressed and significantly affected patients’ prognosis were screened as prognosis-related PCD genes (PRPCDGs).
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PMC11297139
Co-immunostaining of GFP-ARID1A condensate and endogenous BAF showed a notable concentration of BAF subunits (Fig. 2g).
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PMC11085211
Overall, this finding is in line with the evidence that many NPs could exert their anticancer activity by affecting miRNAs expression .
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PMC11055323
Each color represents a pair of homologous chromosomes.
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PMC9429973
Molecular relapse-free survival (MRFS) and overall survival (OS) were estimated with the Kaplan-Meier method.
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PMC8567602
In synovial sarcoma, at TSSs, PBAF exerts a tumor-suppressive function, likely enabling the expression of differentiation genes that affords synovial sarcoma its unique features of both mesenchymal and epithelial differentiation, which is lost upon Smarcb1 silencing/loss.
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PMC9503669
In subsequent work, B9, as well as these first-generation non-azo analogs, were found to restore MHC-I to the surface of HIV-infected CD4 T cells from a large cohort of HIV individuals.
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PMC9429973
In the 1 12 months, median (IQR) duration of exposure to EPAG was 5.6 (2.3-11.9) months and median (IQR) EPAG dose was 57.1 (37.5-69.0) mg/day.
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PMC10092581
Inhibition of TrxR has been shown to dysregulate the cellular redox homeostasis, ultimately leading to cell apoptosis.
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PMC9014716
Chemotherapy resistance is a major burden to the efficacy of therapeutic drugs against hepatocellular carcinoma (HCC).
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PMC11803918
C,D) The percentages of apoptotic A375 cancer cells or HSF skin fibroblasts after hybrid γδ-T exosomes treatments at different concentrations without light irradiation (n = 4). (
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PMC11727145
Of note, the possibility for harvesting tissue from the same donor anatomical sites has also helped to standardize DED tissue preparation further.
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PMC11126803
Previously, ERβ was recognized as a prognostic factor in breast cancer (18), and plays a protective role in a variety of tumors, such as lung cancer, colon cancer, and breast carcinoma (19, 20, 21, 22).
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PMC11748335
LOCK identification was based on chromosomes 1–22.
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PMC11106977
The addition and removal steps of UDP-GlcNAc modification are mediated by two highly conserved enzymes, O‑GlcNAc transferase (OGT) and O‑GlcNAcase (OGA), respectively (Fig. 1).
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PMC11755519
It has been observed that LNMAT2 is packaged into exosomes in a hnRNPA2B1-dependent manner.
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PMC11335267
A possible explanation for this observation is that, when submitted to a HFD regime, the induced expression of the lipogenic gene Fasn in Alx3-deficent mice is lower relative to the induction observed in wild type mice, whereas that of the lipolytic genes Hsl and Atgl is higher.
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PMC11502443
Comparison of GIP(1-42), GIP(1-30)NH2 and Tirzepatide efficacy (log(τ/KA)) at the human WT and E354Q GIP receptors in transfected Cos7 cells (A-E).
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PMC11307990
Moreover, when extended to 12 h of incubation, the fluorescence signal of DNA nanotapes was further enhanced and concentrated in the cell membrane (Figure 4A,B).
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PMC6892818
c Raw data from the experiments shown in (a) and (b) are presented as Cell death fold increase in RLU relative to control, in order to compare the degree of apoptosis induction for mCD40L vs. G28-5 mAb treatment in all RCC lines (and control EJ cells).
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PMC7294030
The DNA content of cells was determined in three independent experiment. *
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PMC11470999
We then also used 5 µM PF treated B16 and A375 cells for varying time, CIP2A mRNA levels did not change (Figure 5E).
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PMC11587541
The telomere primer sequence for both single copy gene (36b4) & telomere are provided in Table 1.
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PMC11765988
MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate mRNAs expressed in the human genome .
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PMC7351993
The TM node uses the received ID number to find the sort time of each event and then sends the sort trigger signal to the sort driver at the calculated timing.
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PMC11701801
Depletion of KREMEN2 (Supplementary Fig. S4D) reduced the viability of SMARCA4/SMARCA2-deficient cells and SS18–SSX fusion cells but not that of SWI/SNF-proficient cells (Fig. 4G).
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PMC9429973
Any patient with known or suspected relapsed or refractory AML, who is less than 22 years of age will be eligible for inclusion in the registry in Europe or in the screening protocol in North America, to provide confirmation of relapse and target expression using standardized methodologies.
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PMC11345828
HCl (2.4 mL) was added, and the reaction was allowed to stir for 1 h. The reaction was quenched with aq.
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PMC9436459
The Yu and Chen groups evaluated the antioxidant properties of their obtained MDAAs in Fe/cysteine-induced microsomal lipid peroxidation assay and found that most of the compounds at concentrations of 10 μM had good activities (Vitamine E as the positive control) .
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PMC9429973
Mus, L.M., et al. (2020), “The ETS transcription factor ETV5 is a target of activated ALK in neuroblastoma contributing to increased tumour aggressiveness.”
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PMC11711345
The anticancer activity of C. cyminum has been revised .
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PMC11106977
To date, information concerning the mechanism underlying substrate recognition by OGA is limited.
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PMC7305184
In each generation efficiency of survival of CTRL () group was compared to samples exposed to electric pulses (EP group, ).
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PMC11730320
Her father suffered recurrent ear infections during childhood and mother had recurrent sinusitis.
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PMC10734950
All grown cell lines were incubated at 48 h with F8 to obtain their CC50 value.
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PMC11679326
FUT8 is highly expressed in patient-derived tumor tissues and glioblastoma cells.
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PMC10919056
Flow cytometry analysis of cell apoptosis after knockdown of GJA5 and GJB1 genes in 786-O and A498 cells, respectively. (*
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PMC11683240
Experiments have been conducted in a serum-free medium to efficiently detect inhibitory peptides/proteins, ensuring they are not missed due to interactions with serum proteins.
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PMC9429973
Survivors after first relapse were less than 20%.
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PMC9596868
E-F) Isobologram analysis and CI versus FA plot for the eight drug combinations of quercetin and MST-312 in OVCAR3 cells.
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PMC11731614
Western blot confirms two EAPs, Mapk1 (via ERK1/2 expression) and annexin-7 in SEC Fr2/3.
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PMC11345828
Compound 26 was able to achieve tumor regression in both hematological and lung cancer xenograft models and demonstrated even more robust effects when dosed in combination with chemotherapeutic agents such as docetaxel or topotecan.
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PMC11599565
However, little is known about the capacity of platinum-based cytostatics to convert ovarian cancer cells into neuron-like cells and, importantly, their molecular characterization, as well as the physiological fate of this induced neuronal cell type.
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PMC11719944
Although the role of hormones in autoimmunity is well-documented, our results did not show significant differences in the serum concentrations of hormones, such as estradiol, follicle-stimulating hormone (FSH), and free T4, contrary to our expectations.
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PMC3765139
Statistical analysis was carried out using ANOVA.
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PMC9412887
Moreover, CEPH and ultrasound showed the strongest antitumor effect due to the synergistic effects of erlotinib and SDT.
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PMC11055510
The research findings may provide experimental basis for clinical application.
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PMC11322866
Scale bar: 10 μm.
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PMC6442998
Despite the introduction of tyrosine kinase inhibitors, gastrointestinal stromal tumors (GIST) resistance remains a major clinical challenge.
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PMC11593084
Systemic administration of NZ-1-based CAR-T cells inhibited intracranial glioma growth in immunodeficient mice .
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PMC9516447
Consistent with this view, a highly desirable target for anti-cancer therapy is a cellular moiety which controls multiple functions required for the cancer cells to proliferate at a high rate.
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PMC11474209
depicts the different advanced biosensors for detecting MC-LR in a water sample and the detection limit.
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PMC11443022
Computed tomography revealed a 27-mm-long calcification in the scrotum (Figure 2A), while magnetic resonance imaging displayed aplasia of the prostate and seminal vesicle glands (Figure 2B).
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PMC11599565
Ovarian surface cells derive from the intermediate mesoderm, specifically from the Müllerian paramesonephric ducts (after the invagination of the coelomic epithelium) whereas the fallopian tube derives from the “primary Müllerian system”.
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PMC11590870
Histidine gives anticancer peptides the ability to induce cancer cytotoxicity by membrane permeability.
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PMC11721587
We speculate that Hh-induced SUMOylation and subsequent SUMO-SIM interaction may promote Fu maturation by inducing Fu self-association to form condensates.
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PMC7841067
For in vitro transfection, ACT-1 cells were seeded into each well of 24-well microplates, grown for 24 h to reach 50% confluence, and transfected with sh-AKAP4 or sh-NC using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
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PMC10523483
Briefly, frozen tissue sections were prepared by embedding fresh tissues in OCT compound (Sakura, CA) and sectioned to 10 μm, then stained with DHE for 10 min at room temperature, and covered with DAPI and antifading medium.
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PMC10747139
BOLD-100 demonstrated a significant glycolysis-blocking anti-Warburg effect as a novel mechanism of action.
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PMC3164356
Finally, the program cuffcompare (part of the Cufflinks suite) was used to compare and reconcile the protein sequences predicted from Cufflinks and BestORF and the Glean annotation.
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PMC10994876
p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001) Cell cycle distribution determined by flow cytometry; cell count vs. fluorescence.
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PMC9429973
4 patterns were observed: inactivation of cluster 1 genes such as ZEB2 or IKZF1 did not change cell differentiation or growth, loss of cluster 2 genes like RPS21 or RANGAP1 did not change differentiation, but impaired cell growth/survival.
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PMC10809689
RP-HPLC was done on a C18 column (150 * 4.6 mm, 5 μm), using Mobile-phase A (1 g K2PO4 + 0.5 g KH2PO4 in 500 mL distilled water), and B (100 mL distilled water, 150 mL acetonitrile, and 300 mL isopropanol) with linear gradient from 10–100% B at a flow rate of 1.2 mL/min in 35 min Changes of glutamate level in control, PMA, and PG72 samples of HL-60 cell line and RP-HPLC chromatograms.
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PMC11718809
Figure 5B).
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PMC10545062
To create a reproducible shock state where swine are consistently fluid avid and hypotensive with consistent peak lactate levels, an autonomously controlled model incorporating hypovolemic shock exacerbated by ischemia-reperfusion injury was used.
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PMC10165258
Cells are seeded and 24 h later, when EwS spheroids are formed, they are exposed to both drugs at specific concentrations and imaged every 24 h.(C)EwS spheroids viability heat maps over 6 days.
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PMC9429973
TKI dose reduction consisted of 2 steps each lasting for 6 mo.
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PMC4005030
Thus PCA may find a valuable use in management of Parkinson's disease.
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PMC8316344
Here, we investigated the functions of a serial of iron regulators during the process in which TRIB2 reduces labile iron.
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PMC9786247
As compared with epithelial cells from other outer surface linings of cattle (lung, gut, placental), C. burnetii strains Nine Mile I and II exhibit a prime tropism for bovine mammary gland epithelial cells in vitro .
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PMC9429973
No death for CRBSI occurred.
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PMC11802929
TCR-related physicochemical properties were computed for each cell based on its TCR CDR3 sequence of the beta chain.
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PMC9429973
91% of all patients with iMCD reported experiencing at least one symptom in the week prior to survey completion.
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PMC11228511
Percentage of cells with an abnormal nuclear lamin meshwork in WT-SMCs, progerin-SMCs, and progerin-SMCs expressing lamin B1, LAP2β, or nf-lamin B1.
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PMC11655498
Therefore, the host proteins interact with viral IE proteins and modulate their activities, potentially depending on the post-translational modifications, which play crucial roles in EBV reactivation.
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PMC9812014
These alkaloids were also non-toxic to the tested cell line .
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PMC6160470
To test the inhibitory potential of crown ether compounds towards P-gp we used 10 µM concentration of all compounds.
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PMC11534377
Quantitative analysis of these results, presented in panels B and D, confirm the increase in both early apoptosis and late stages of necrosis, suggesting that the combined treatment has a greater effect on reducing cell viability than either treatment alone.
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