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OTAR3088
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BioData-CellLine_annotated

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PMC2247044
The cell cultures after the sixth passage were devoid of contaminating fibroblasts as judged by the positive staining of all cells with the specific epithelial cell markers carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and cytokeratin 8.
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PMC11786767
B and C: Distribution of biotin-DHPE and PS.
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PMC11724582
The protein expression levels of α-SMA and FAP in the CAF-48 h group were significantly greater than those in the NF group.
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PMC11209164
This data underscore TPF’s potential role in not only oncolytic virotherapy but also in the broader application of gene therapy.
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PMC11721277
Based on the results of the radiolabel optimization study, an incubation time of 85 °C and incubation temperature of 15 min were determined to be the optimal conditions (Figures S8 and S9).
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PMC10852540
Protein isolation was done from the samples 72 hours after the application.
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PMC9424261
H Total ubiquitination of GSS, as co-IP by anti-GSS antibody for the indicated treatment in Bel-7402 cells with or without HERPUD1 knocked out, and measured by anti-total ubiquitin antibody.
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PMC10988555
The cellular uptake was studied by ICP-AES.
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PMC7612475
To directly determine the impact of the SF3B1 mutation on HR, we assessed the formation and resolution of Rad51 foci, a key marker of HR function, in our isogenic models following IR-induced DNA damage.
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PMC11723947
h–k PD-1 and LAG3 expression on naive CD4 T cells before or after two days of culture with anti-CD3 antibody.
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PMC11725127
Mutant KRAS is correlated with reduced overall survival, elevated tumor aggressiveness, and increased tumor burden in MM patients, underscoring its pivotal role as an oncoprotein in the pathogenesis and progression of MM11, 12, 13.
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PMC11795610
Myricetin was dissolved in DMSO and diluted to the desired concentrations.
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PMC7039683
For analysis of the potential clustering pattern of periderm-enriched motif combination within zebrafish GPAEs, we firstly annotated all the GPAEs using HOMER annotatePeaks function with the motif files for GRHL, TEAD, KLF, FOS, TFAP2, GATA and CEBP, and counted the occurrence of each motif in each peak.
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PMC9429973
Methods: We performed RNA-sequencing on a cohort of 28 PTCL cases.
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PMC9429973
Overall, 44/45 (98%) pts achieved marrow U-MRD as best response at any time during the study.
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PMC11488203
The conditioned medium from taxol-loaded MSC was concentrated about 100-fold in an Amicon® Ultra-15 centrifugal filter device (Millipore/Merck/Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany), (molecular weight cutoff 100 kDA) equilibrated with αMEM medium at 3,200 g for 25 min.
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PMC4270159
All cells will be sent for mycoplasma testing and STR profiling.
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PMC10898159
ETV1 is also a master regulator of the ICC lineage, and it is essential for the development of the subtypes of ICCs which are sensitive to oncogenic KIT-mediated transformation.
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PMC11774747
Moreover CAR-VST are associated with a low incidence of GvHD, a common complication expected with traditional allogeneic CAR-T cells.
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PMC9370419
Briefly, the cells from transfected HepG2 cells on a 6-well plate were lysed in 400 µL lysis buffer (10 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM EDTA, and 1% NP-40), precipitated with 125 µL of 26% PEG solution, followed by 10 U DNase I digestion for 2 h, a 200 µL of Proteinase K Buffer (35 mM Tris-HCl, pH 7.9, 35 mM EDTA, 175 mM NaCl, 1.5% SDS, and 1.35 mg/mL Proteinase K) digestion for 6 h, phenol extraction, and then ethanol precipitation.
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PMC9436459
Protein tyrosine phosphatase 1B (PTP1B) is a key negative regulator of insulin and leptin signaling pathways.
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PMC4395774
After 16 weeks, animals were infected with LCMV-WE (200 pfu, i.p.).
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PMC11623887
HEK293 cells were transfected with pHI0 plasmid containing the B19V genome and the plasmid of the GATA1 genome.
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PMC11714165
Bioactivity of fluorous NBD nanoparticles.
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PMC11756907
The use of Go-2ME and rGO-2ME as drugs in anticancer therapy for melanoma is a solution to the problem of the toxicity of 2-ME itself towards healthy skin cells.
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PMC11792888
Figure 3.
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PMC11397654
At the same time, it was assessed that the mechanism of action of the compounds was cytotoxic, not cytostatic, as the number of cells was reduced after treatment.
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PMC11519583
The membranes were blocked with 3% (v/w) BSA in TBST (TBS [137 mM NaCl, 2.7 mM KCl, 25 mM Tris] containing 0.05% [v/v] Tween-20) for 1 h, followed by immunoblotting with primary antibodies (Supplementary Table 1) at 4 °C overnight.
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PMC9429973
8 pts (53%) were newly diagnosed and 7 pts (47%) were previously treated, with a median of 1 (1-3) prior therapies, including 2 (33%) with prior SCT.
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PMC10114490
Although HLC do not yet display a fully mature phenotype, they outperform many other liver cell models and the degree of differentiation achieved is generally sufficient for most study goals.
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PMC11704834
The E-cadherin upregulation by gefitinib treatment coincided with a significant re-localization of the nuclear TLE1 protein to the cytoplasm as evidenced by subcellular fractionation and western blotting assays (Fig. 3B), consistent with the notion that the observed gefitinib-induced E-cadherin expression is associated with inhibition of TLE1 nuclear function.
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PMC10728200
Lipid peroxides are the result of oxidative damage to polyunsaturated fatty acids (PUFAs) caused by reactive oxygen species (ROS).
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PMC11655536
We measured the lysosomal membrane stability by AO staining, which emits green fluorescence when acid substances are released from damaged lysosomes.
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PMC11525028
Sequencing data (accession number GEO: GSE237543) have been deposited at GEO and are publicly available as of the date of publication.
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PMC10158546
Images were taken with a phase contrast microscope 3 days following puromycin treatment. (
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PMC11422150
Three days after induction of SCI with forceps calibrated with a 0.2 mm gap, hAMSCs or vehicle were administered intravenously.
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PMC9429973
Inhibition of EZH2 activity is a therapeutic vulnerability in cancer cells.
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PMC9429973
WBC counts were normal in 63.6% and high in 24%.
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PMC9817179
Thus, our model predicts that TAF15(IDR)-Halo-FTH1 has more stable homotypic interactions than Halo-TAF15(IDR), resulting in a higher relative overall fixation rate of the former than the latter.
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PMC11490153
In A-375 cells, treatment with C. citratus-AlgNPs under hypoxic conditions led to significantly more apoptotic cells (P < 0.05) than all other samples under normoxic and hypoxic conditions.
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PMC11021472
Mean expression of a representative experiment is shown with upper and lower limits.
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PMC10547921
It can inhibit the polymerization of tubulin and induce cell apoptosis and has a strong anti-proliferation activity on cancer cells including multi-drug resistant KB-V1 cells (IC50 = 0.08 nmol/L), which has a good prospect in the development of anti-cancer drugs.
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PMC9429973
Rituximab combined with cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) was defined as 1-line therapy, other therapies than R-CHOP were defined as 2-line therapy.
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PMC11632064
See also Fig S3. (
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PMC11470381
Merge the two fluorescence channels.
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PMC8345486
Compound 24: Yield: 15%.
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PMC11770130
For studies of WT obese animals, 8-week-old male mice were fed by HFD for 8 weeks before the experiments started.
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PMC9429973
MajesTEC-1 is a single-arm, phase 1/2 study in patients with TCE RRMM, previously exposed to an immunomodulatory drug, a proteasome inhibitor, and an anti-CD38 antibody and received ≥3 LOT.
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PMC11789597
CAV1 knockdown decreases MM cell adhesion to stromal cells and attenuates cell adhesion‐mediated drug resistance to bortezomib.
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PMC11707832
In a genome-wide RNAi screen conducted by Alexander Karlas et al. , knockdown of MAN2B1 using siRNA inhibited the replication of influenza A/WSN/33 or A/Hamburg/04/2009 viruses in A549 cells, without affecting cell viability.
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PMC11317131
In the K562 lymphoblast cell line in particular, evolved integrases had a 11-fold increase in targeting efficiency over wildtype.
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PMC9874064
Therefore, in order to evaluate the ability of cancer cells to internalize NP-Pt-USP1i, a fluorescent dye of Cy5.5 was used to label the nanopoarticles of NP-Pt-USP1i (NP-Pt-USP1i@Cy5.5).
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PMC11785489
Recognizing that SARS-CoV-2 infection is a potential confound, we filtered to individuals that were PCR negative for SARS-CoV-2 but continued to observe enrichment in both dataset 1 and dataset 2 (13 matches versus 6 expected by chance, p = 0.00048, exact binomial test; 11 matches versus 6 expected by chance, p = 0.00138, exact binomial test).
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PMC10870498
The GSE85457 dataset was acquired from Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/).
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PMC11357960
The relationship between the SUMOylation inhibition and the phenotype of lymphocyte. (
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PMC11264242
GAPDH serves as the loading control.
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PMC11365983
These reveal shortening of the cilium (red) with shRNA inhibition, and the converse with OE. (
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PMC9429973
Aims: To evaluate the concordance and performance of physician-assessed response in the COTA real-world database to derived response using the 2017 ELN criteria as the established standard for response assessment in clinical trials.
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PMC6461034
○For splitting, dissociate organoids using shear distress (pipetting), spin down cells, and resuspend in basement membrane extract hydrogel.○For viability assays: ‒On day 0, prepare single‐cell suspensions using shear distress (pipetting) and trypsin‐EDTA treatment.‒Seed cells in multiwell plates coated with basement membrane extract hydrogel and containing the above medium without EGF.‒On day 1, add drugs.‒On day 6, assess cell viability with a CellTiter‐Glo luminescent cell viability assay.
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PMC9429973
The hematological ORR was 74.1% at 3 months (40.7% with CR and 18.5% with VGPR).
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PMC11532793
paired-end reads over samples.
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PMC11461833
In contrast, 662 upregulated genes and 1882 downregulated genes were identified in WM-3835-treated late hHEPs (compared with untreated late hHEPs), as shown in Fig. 1B, with STXBP5 and STXBP6 among the downregulated genes.
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PMC7090062
P < 0.05, **P < 0.01 vs. IM.
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PMC10197932
The samples were collected as part of NETWORK TELETHON DI BIOBANCHE GENETICHE [Genomic and Genetic Disorders Biobank] the use of volunteers' blood samples was approved by the institutional review board at IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, ethics committee cod.
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PMC10203589
These results suggest that FGF18 can improve the survival of HCC cells under hypoxia or nutrient deprivation.
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PMC11610461
Subsequently, we compared the tumor and paracancer tissues from KIRC specimens of four different tumor stages (Fig. 2A).
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PMC11476837
The bands were visualized with Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) and Gel-Pro Analyzer (Microchem Laboratory, Round Rock, TX, USA).
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PMC10823017
The Cleaved Caspase 3 (Asp175) antibody (MAB835) was purchased from R&D systems (Minneapolis.
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PMC11473843
The HBEC3-KT cells were cultured in KSFM (Gibco) supplemented with EGF, Bovine Pituitary extract (Gibco) (Ramirez et al, 2004).
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PMC9429973
After blinded relabelling of all the slides, the nine reviewers repeated the plasma cell quantitation using both methods.
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PMC7553912
In addition, in vitro study revealed that OSM directly induced the expression of TGF-β in the RTECs, while αSMA was not affected by OSM in the renal fibroblasts.
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PMC10813895
In normal breast tissue, ER− luminal epithelial cells progenitors exclusively express ELF5 .
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PMC11806818
However, the clinical applications of these methods face significant limitations due to low sensitivity, complex and time-consuming procedures, the need for large sample sizes, and high costs .
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PMC8956657
Eluted proteins were pooled and concentrated to 10–20 mg/ml using 10 kDa MW centrifuge filters.
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PMC5673060
Network maps represent clusters of annotation terms from different databases with associated function.
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PMC11488203
However, the use of MSC-derived EVs/exosomes as a vehicle to deliver tumor-therapeutic compounds may not apply to all kinds of substances and therefore predominantly displays drug-specific properties.
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PMC7762347
Moreover, the % of cells in each phase of the cell cycle (G0/G1, S, and G2/M) was evaluated by FlowJo Software using Dean jet fox model (Single Cell Analysis Software v10, FlowJo, LLC).
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PMC11244823
For Fig 8, the stratified median reactions of each individual serum against each antigen were plotted.
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PMC11317131
These systems are employed naturally in a variety of cellular processes, including bacterial genome replication, differentiation and pathogenesis, and movement of mobile genetic elements (19).
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PMC11658074
Scale bars = 50 μm. *
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PMC9844987
Median promoter variability (line) and interquartile range (shading), as a function of the number of GTEx tissues the associated gene is expressed in (median tissue expression >5 RPKM). (
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PMC11401350
For angioimmunoblastic T cell lymphoma (AITL), a rare cancer, no specific treatments are available and survival outcome is poor.
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PMC11676169
We thus explored the BCAT1 interactome by immunoprecipitation (IP) using tandem affinity purification (TAP) of double-tagged BCAT1 (BCAT1 myc-DDK) (Figure 2A).
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PMC11634027
This mispolarization is consistent with the observation that PCP is abnormal in ds.
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PMC10812665
Similar data were obtained for MPP and Rot (Supplementary Table S1, Figure 7D).
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PMC11684821
Error bars depicting SEM are displayed when triplicate conditions were performed.
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PMC7823217
Fig. C is reproduced from Ref.
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PMC11721295
To understand how oncogenic KRAS regulates the composition and differentiation status of macrophages, we used ex vivo approaches.
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PMC10743717
This subcomplex can bind to a single-subunit protein-only RNase P enzyme (PRORP also known as MRPP3) to form mitochondrial RNase P .
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PMC5941560
The GATK variant quality score recalibration (VQSR) scheme was run for both SNPs and indels, and only the last tranche was filtered out of the call set with the -ts_filter parameter set to 99.9.
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PMC8777939
NpFlamma HGC ICG (PNC1501) was purchased from BioActs (Incheon, South Korea).
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PMC9429973
The degree and course of cardiotoxicity seem to differ significantly between our patients, suggesting that both the underlying genetic predisposition and the presence of various other risk factors contribute to the severity of its manifestation.
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PMC10854447
Found: C, 62.45; H, 3.85; N, 20.42.
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PMC9847912
Similar results were obtained in two of three assays with HIV-2ROD9; in the third experiment, the level of infectious virus at 40 nM GSK232 was 46% of that found in the no-drug control wells.
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PMC9429973
Online CME is valuable in improving knowledge, competence and confidence, as well as identifying areas of future educational need and potentially improving patient outcomes.
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PMC4277423
B. T7EI analysis of nine potential off-target loci (SH2D5, ASB9P1, PRRT1, LINC00265, ENDOV, NR2F1, ASB9, CLPP and SOBP) to CR3 as well as on-target locus CCR5 control. “–”:
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PMC9429973
Higher levels of activated VLA-4 were found in CLL using the IGHV3 and IGHV4 families, compared to cases using the IGHV1 family (p=0.043 and p=0.004).
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PMC11476837
The level of apoptosis in doxorubicin-sensitive HL-60 cells was significantly higher than in HL-60/DR-resistant cells (p < 0.001).
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PMC11792888
This agent, inhibits topoisomerase II leading to the formation of single- and double-strand DNA breaks , inducing cell cycle arrest at S and G2/M phases, followed by apoptotic cell death.
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PMC8409415
All animal experiments were conducted according to the institutional ethical guidelines for animal experimentation of Osaka University (registered protocol number 28‐032‐013).
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