PMCID
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PMC10759659
A total of 108 HBV-infected patients were enrolled from the TCGA-LIHC cohort after removing duplicates and patients with mutations or missing clinical information.
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PMC10810426
Western blots demonstrated that the nuclear and cytoplasmic fractionation was successful (Fig 2B).
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PMC11319300
Data is visualised using the WashU Epigenome Browser. (
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PMC9429973
Results: With our approach, we are able to preserve the tissue composition and the interactions between tumor cells and their microenvironment ex vivo for three to four weeks.
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PMC11720808
MG63 and U2OS cells were transfected with NF-κB p65-responsive luciferase reporters and then treated with SCU, after which cells were collected for dual-luciferase activity assay.
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PMC8540692
EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA) was used for sodium bisulfite treatment, i.e., deamination of unmethylated cytosines within CpG dinucleotides to uracil while leaving the 5-methylcytosines intact.
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PMC9966931
However, the method used in this study required subsequent purification processes in order to obtain large quantities of pure hybrid compounds based on the constituents of T. vulgaris and C. verum EOs.
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PMC6190245
The data are expressed as mean±SEM (n=3). *
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PMC11726183
The grid for the docking study was generated at the site where erlotinib was bound as the co-crystal ligand.
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PMC10125312
For MTT assay, 50 μL of MTT 5 mg/mL was added to the remaining 450 μL of cell suspension and incubated 30 min at 37 °C.
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PMC11704834
no. 610181) and anti-vimentin (1:1,000; cat.
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PMC11591030
All the graphs were drawn on GraphPad Prism (version 8.0, GraphPad Software, San Diego, CA, USA) and Photoshop software (version CS6, Adobe, San Jose, CA, USA).
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PMC11541409
Kurokawa, Y., et al., Pimitespib in patients with advanced gastrointestinal stromal tumor (CHAPTER-GIST-301): a randomized, double-blind, placebo-controlled phase III trial.
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PMC8759873
Remove the culture plate, add MTT 5 mg/ml to each well, and place in 5% CO2 incubator for 4 hours.
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PMC11739504
However, the intensities of IFT46 and IFT140 were not changed in Kat2b-depleted cells (Supplementary Fig. 2a,b).
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PMC11715644
In previous studies, cell elongation has been calculated as aspect ratio (AR), and values of shape index (SI; perimeter divided by square root of area) ≥3.81 indicate unjamming.
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PMC8873393
Two of the factors CSA and CSB involved in the TC-NER pathway were initially characterized by their association with an inherited syndrome, namely Cockayne syndrome (CS) .
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PMC11724582
The values are the means ± SEMs.
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PMC11794847
A: The silver staining image of IP-MS with HVEM protein in Jurkat T cells; B: COIP assay indicated that HVEM could interact with FOXA2; C: Immunofluorescence staining confirmed the co-localization of HVEM and FOXA2 in the cytoplasm; D: Molecular docking revealed the interaction between HVEM and FOXA2; E: Dual-luciferase activity analysis showed Si-FOXA2 could increase the luciferase activity of GPT2-WT but not that of GPT2-MUT; F: GPT2 mRNA was upregulated after FOXA2 knockdown; G: HVEM knockdown had no infouence on the mRNA expression level of FOXA2;H: Nuclear-cytoplasmic fractionation assay results We used JASPAR and QIAGEN website to predict the possible upstream transcription factors and GATA1 belonged to the intersection of the two prediction tools.
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PMC11536589
Each group had four mice.
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PMC9848491
The number of dead and alive patients with different risk scores.
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PMC11420784
P values were determined using Student t test.
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PMC11742231
Each atom node was described using a 78-dimensional feature vector.
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PMC10958426
We plotted interaction weight between cell types by netVisual_circle function.
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PMC9777812
As a result, over-expression of bta-miR-106b merely down-regulated the GAPM expression (Figure 5A; p < 0.05) and bta-miR-106b inhibitor merely up-regulated the SCD expression (Figure 5B; p < 0.01), suggesting that bta-miR-106b had little effect on milk fat synthesis and metabolism.
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PMC9429973
Methods: Bone marrow aspirates were collected from KarMMa pts at pretreatment (n=97 RNA, n=68 DNA) and progression (n=64 RNA, n=33 DNA).
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PMC9429973
Results: Within our ALL cohort, 14% of the patients met the criteria for t-ALL.
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PMC11297139
Scale bar: 5 μm.
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PMC11144200
This synergistic effect of chemotherapy combined with immunotherapy may be involved to the mechanism by which inducing immunogenic cell death (ICD).
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PMC11463018
H NMR (500 MHz, DMSO-d6) δ: 7.59 − 7.58 (m, 1H), 7.49 − 7.44 (m, 2H), 7.34 − 7.29 (m, 1H), 7.08 − 7.05 (m, 1H), 7.00 − 6.97 (m, 1H), 6.12 (s, 2H), 6.03 (s, 2H).
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PMC9429973
Results: As of February 25, 2022, a total of 10 pts have been enrolled (9 in dose escalation, 1 in dose expansion): 7 pts with CMML, two with MDS with excess blast and one with atypical chronic myeloid leukemia (aCML).
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PMC9429973
Median progression-free survival and overall survival were not reached.
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PMC11705862
Error bars indicate standard deviation (SD).
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PMC11298021
Interaction was indicated by a score of 1 and its absence by a score of 0.
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PMC10747139
Finally, and very importantly, dual-active drugs are a concept that has been noted as an imperative in future drug design.
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PMC10667689
Also, switching to FAS and upregulated ACC-1 upon DC activation and M1 macrophage polarization has been reported previously (44–49, 51–53, 55, 59).
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PMC10858941
About 250,000 cells were seeded on a p35 dish.
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PMC9784246
The absorbance of each well was measured at 540 nm using a Molecular Devices Versamax tunable microplate reader.
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PMC11528603
A: HL60 cells treated with SA-EA (4.1 μg/ml) and 0.05% DMSO (control) for 96 hours.
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PMC10421378
DSC analysis of cell denaturation was used to determine the temperature at which denaturation started in our samples, which was between 65 and 70 °C.
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PMC5034105
We showed that the renal (HEK) and respiratory tract (Hep-2, Calu-3, HFL) cell lines were also susceptible to ZIKV.
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PMC11683240
Cells were left untreated as control (Con).
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PMC11799978
Once the cells adhered to the wall, 80 μg/mL NaHCO3 was added to one of the dishes.
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PMC11705630
The results suggested that depletion of GLDC in ACHN cells induced enlarged nucleoids (Fig. 1D).
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PMC11472569
In addition to ABC transporters, other proteins transported via EVs may also significantly contribute to the development of MDR.
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PMC7553912
Approximately 80% of kidney stones are composed of calcium oxalate (CaOx).
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PMC11786767
Relative distribution patterns of Ki-67, biotin-DHPE, and PS.
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PMC11637716
While the other clinical symptoms have been repeatedly described in other SDS patients, such lung involvement has not been reported previously.
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PMC9872547
Briefly, an esterification reaction of oleoyl chloride (0.01 mol) and polyethylene glycol 400 (0.01 mol) in the presence of triethylamine (0.012 mmol) and chloroform was performed for the synthesis of OA400.
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PMC10877303
Additionally, numerous comparative studies have been carried out on rhesus monkeys, Ad5-hCD26-transduced mice, and twelve healthy common marmosets .
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PMC9429973
17 and MEF2B on chr.
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PMC11247842
Most SNPs that were significant in both the meta-analysis/multi-trait analysis and the eQTL analysis were trans-eQTL (on different chromosome) (see Additional file 3: Table S11).
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PMC9429973
SF3B1 mutation was recorded for 7.5% US/CAN and 8.2% UK/EU pts.
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PMC10577955
A Control cells, B ALUP, C BA, D LUP RT-PCR results revealed that BA treatment significantly raised the expression of let-7 miRNA (3.23 ± 0.17) in A549 cells compared to other treatments at P < 0.001.
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PMC10899471
A–D) qPCR and Western blot analysis of the expression level of BATF2 in soft tissue sarcoma and pericarcinoma (A, B) and the human skin fibroblast cell line (HSF), human fibrosarcoma cell line (HT-1080) and synovial sarcoma cell line (SW-982) (C, D). (
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PMC8903741
To grow the cell line, we supplemented an antibiotic geneticin (G-418) to the media since pcDNA3.1(−) includes a neomycin resistance gene (neo).
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PMC11532793
Applications of our recommended pipeline to the human adenocarcinoma cell lines dataset resulted in faster and more powerful analyses with several DE transcripts, including novel transcripts associated to key cancer-related genes that were not previously detected with Salmon’s bootstrap sampling.
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PMC10470467
The relation between methylation and gene expression. (
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PMC11772449
Although there is no specific value as a standard at present, the interpretation standard of synergy score can be determined according to experience: less than −10, there may be antagonism between the two drugs; from − 10 to 10, there may be a simple addition/subtraction effect between the two drugs; more than 10, there may be a synergistic effect between the two drugs.
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PMC11686380
Expression of GAP43 and GAP43-E111K in vivo in mouse cortical neurons.
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PMC11617590
Subsequently, we measured the level of cMyc, the target gene of β‐catenin.
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PMC11286266
ATF6αΔ cells were re-targeted with a sgRNA/Cas9 directed to the mutated exon 10 and offering three repair templates: ATF6α_LD in red, ATF6α_LD in green, and ATF6α_LD in purple.
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PMC9429973
After inclusion of the MDS-CI as a categorical variable (reference group low-risk) into a Cox regression model together with the DIPSSdich, the MDS-CI retained its independent prognostic value (MDS-CI intermediate HR 2.05, 95% CI 1.0-4.15, p= 0.046 and MDS-CI high HR 13.2 (95% CI 4.0-43.1, p<0.001).
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PMC11770199
Wong et al. incorporated the p14 fusion-associated small transmembrane (FAST) protein from reptilian reovirus into oncolytic adenoviruses, promoting virus replication and spreading.
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PMC9429973
Functional characterization of MDSCs and Tregs by transcriptomic (RNAseq) and proteomic analysis are ongoing.
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PMC10907726
p < 0.05 (with NC).
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PMC9926039
Naloxone hydrochloride (Caspian Tamin Co., Iran), morphine sulfate (Darou Pakhsh Co., Iran), and methadone (Exir Co., Iran) were used for the A549 cell line experiments, in vitro.
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PMC11782508
In addition, the presence of the azurin gene in the isolate’s genomic DNA was verified using PCR (Fig. 1b; Supplementary Fig. S1), and the amplicon sequencing revealed 99.65% similarity with the published azurin.
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PMC6642070
In accordance with recovered exercise capacity, stopping administration of lovastatin decreased blood lactate levels back to normal (Fig. 1b).Fig.
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PMC5308810
The results are presented as mean ± SD.
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PMC11721064
Fluorescent signal quantification in three randomly selected image areas was performed using ImageJ software (National Institutes of Health, USA).
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PMC11623887
Erythropoietin signaling is an important pathway in EPCs maturation to erythrocytes and is an as crucial factor in enhancing B19V replication.
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PMC11478724
The study also performed Acridine orange (AO) and ethidium bromide staining on the A549 cell line, capturing images with fluorescent microscopy.
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PMC11726848
During irradiation, cell culture confluence did not exceed 50%.
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PMC4816271
Whilst the PVAA-containing CAR lysed GD2-expressing LAN1 cells with equivalent efficacy to wild type, the PVAA CAR had significantly reduced off target cytolysis against CD64 expressing THP1 cells (p = 0.0005 Fig 3D).
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PMC10729469
However, with disease progression microglia become more activated and acquire amoeboid morphology with compromised phagocytic capabilities and elevated neurotoxicity .
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PMC11657695
The expression of two thirds of these genes are significantly decreased in the siRNA KCNA2-transfected cells (Fig. 5D).
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PMC10502403
TCRγδ-specific agonistic mAb- and NE1-pMHC-coated plates were used for NGMCs and NE1-GMCs, respectively.
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PMC9429973
Upon completion of dosing, or if a patient terminated the study early, a follow-up visit was completed 9 weeks after administration of the last dose of sutimlimab.
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PMC9429973
All statistical analyses were conducted with the Mann Whitney U test.
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PMC10606998
The process of formation of electron–positron pairs occurs at energies exceeding 1 MeV—usually 2 MeV or more .
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PMC11661040
Our findings demonstrated that CGREF1 did not directly influence the migration and invasion capabilities of osteosarcoma cells.
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PMC4485648
The human ovarian cancer cell line A2780 and derived cell lines resistant to adriamycin A2780/ADR and cisplatin A2780/CP were described earlier (19,20).
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PMC11665584
Additionally, a few (470 control, 775 PITX1) skin cells clustered with salivary cells.
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PMC10218459
Subsequently, 800 µL of warm full medium containing the corresponding cytostatic agent, dissolved at their determined IC50 values, was added to each well and incubated at 37 °C.
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PMC7504302
Furthermore, the high expression levels of EIF3A gene seemed to correlate, although not significantly, with lower overall survival rates (Figure 1).
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PMC11604015
In 2021, Pan et al. demonstrated a CAR-T cell product that could retain de novo-synthesized CD7 proteins in the ER, thus minimizing the expression of surface CD7.
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PMC10547921
Chemical structures of cryptophycin analogs 35–40; (B) Binding mode of cryptophycin-52 (36) in complex with tubulin (PDB code 7M20).Figure 7 Design and SAR analysis of cryptophycin analogs. (
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PMC9429973
Aims: We aimed to characterize pre-PMF patients referred to our institution between the years of 2000-2020.
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PMC10530622
The authors also found out that BMAL-1 polymorphysms were associated with CaOx stones by analyzing blood samples from kidney stone patients .
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PMC11703992
D The friend analysis of significant genes.
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PMC11224206
Mitochondrial motility has been well-summarised recently by Harrington et al. .
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PMC11725127
GA has been reported to induce the apoptosis of MM cells by inhibiting the PI3K/Akt/mTOR, NF-κB, and other signaling pathways.
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PMC10887351
Besides the myocardium, in the 1970s, some studies suggested the presence of a peculiar β-AR in human detrusor , which, through adrenoceptor signaling and sympathetic nerve activity, promoted urine storage by relaxing the detrusor muscle.
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PMC9429973
Summary/Conclusion: Our findings indicate a yet unrevealed participation of the BMAT niche in compartmentalization of adult hip bone hematopoietic progenitors by hosting a set of hematopoietic cells and potentially, BMAT progenitors in the close proximity of BM adipocytes.
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PMC11354225
In the particular case of NMSC, it is subdivided into basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) or epidermoid, constituting 80% and 20% of cases, respectively.
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PMC9429973
Patients with chromothripsis showed a higher frequency of TP53 (70% vs 39%; p=0.001) and a shorter TTFT (15m vs 2m; p=0.013) than the control group.
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PMC11597291
The nCGO and nCGO-PEG spectra exhibited a peak at 2.21 ppm attributed to the nCGO methylene (-CH2-) protons accompanied by varied weak peaks in the range 1.0–2.0 ppm of the aliphatic area attributed to the CH-CH protons of GO.
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PMC10291556
On the other hand, it cannot be entirely excluded that improvement and enrichment of the activity of the original parent Pt(II) complex 1 are achieved without the release of free DCF molecules from the Pt(IV) complexes.
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PMC6181431
D, E) These same A704 derivative cell lines were plated at 200 cells per 10cm dish and treated with or without Doxycycline and DMSO control or Rapamycin (20nM) for 30 days, then formalin fixed and stained with Crystal Violet.
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