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PMC11680049
For primary screening of fungicidal activity, concentrated ethanol extracts obtained from raw biomass of shoots and callus tissue of S. sempervirens weighing 1 g were used.
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PMC9429973
Expression and activation (phosphorylation) of cGAS-STING pathway proteins at baseline and experimental conditions were analysed by quantitative RT-PCR and Western blot analysis at the RNA and protein level, respectively.
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PMC8996378
Seventeen new jatrophane diterpenoids and five known were isolated from the whole plant of Euphorbia esula (Table 1).
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PMC9429973
Summary/Conclusion: In this, one of the largest real-world HR MDS cohorts, CR and OS with AZA in treatment-naive patients were limited.
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PMC11489175
Efforts have been made on pharmacological targeting of USP1 119-126 and they may pave the way to new opportunities for ovarian cancer treatment.
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PMC11093197
We observed a similar phenomenon in human mammary epithelial cells (HMEC) as well.
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PMC11746942
A Macroscopic appearance of dorsal skin (top row), skin-draining lymph nodes (middle row), and histological staining (H&E) of the back skin of WT mice and S1PR3-KO mice with and without IMQ treatment.
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PMC11078693
Resuspend the cell pellets into the remaining 2–3 mL of the supernatant.d.
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PMC11026382
Additionally, mutations with the largest magnitude epistatic effect to TYMS center around the DHFR active site, while more weakly epistatic positions radiate outwards.
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PMC11754291
By contrast, our study incorporated primary cells from four diverse donors, representing a range of Fitzpatrick phototypes and anatomical sites.
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PMC9895440
Following incubation, cells were washed, resuspended in the same buffer and filtered through a 70 μm mesh.
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PMC11762280
Two investigators blinded to study subgroups scored the severity of skin lesions in all groups.
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PMC11726848
The level of hydrogen peroxide in cells after irradiation was assessed using the fluorescent probe DCFH2DA.
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PMC9429973
H. Fokken, N. Kattre, A. Kloos, M. Heuser, T. Kacprowski, M. Tobias, A. Schwarzer Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover; Division Data Science in Biomedicine, Peter L. Reichertz Institute for Medical Informatics of TU Braunschweig and Hannover Medical School; Braunschweig Integrated Centre of Systems Biology (BRICS), TU Braunschweig, Braunschweig; Department of Pediatric Hematology; Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany Background: The detection of residual leukemic cells (“measurable residual disease”/MRD) has dramatic prognostic impact on relapse incidence and patient survival in AML and has become key to guide therapeutic decision making.
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PMC11723947
Finally, gene expression is quantified with Salmon v.1.5.2.
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PMC11594588
The oxidative stress is the disturbances between all repair pathways, systems in the organisms, and their homeostasis .
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PMC10758402
Additionally, MMF, the biologically active metabolite of DMF, did enhance HSV.n212 as shown in ( Figures 3A, B ).
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PMC11763126
Therefore, chemical modifications are necessary to enhance their potential for clinical use.
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PMC11750712
AUTO4 was safe with minimal toxicity observed.
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PMC10605143
On the other hand, the down-regulation of HSF1 in MCF12A cells (which were initially basal-like) led to the enrichment of the luminal progenitors’ subpopulation.
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PMC11424574
Knockdown of MTA2 inhibited osteosarcoma cell migration and invasion by reducing the expression of urokinase-type plasminogen activator (uPA).
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PMC11756514
The library consists of approximately 2,000 mutants, each with one nonessential gene disrupted by insertion of the mariner transposon bursa aurealis harboring an erythromycin (Em) resistance gene.
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PMC9454178
Furthermore, we performed correlation analysis of OSR1 and c-KIT expression, and the results showed that OSR1 levels were positively associated with c-KIT levels (r = 0.407, P < 0.001, Fig. 8G).
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PMC11674891
Correlation analysis was performed using R (http://www.r-project.org) (accessed on 18 December 2024), and graphs and heatmaps were generated using Microsoft Excel 2010 and R 4.4.1.
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PMC11618052
H NMR (400 MHz, δ ppm CDCl3): 8.15 (dd, J = 8.0, 1.1 Hz, 1H, Ar–H), 7.99 (s, 1H, triazole CH), 7.69–7.61 (m, 1H, Ar–H), 7.55 (d, J = 8.8 Hz, 2H, Ar–H p-Cl C6H4), 7.52 (s, 1H, Ar–H), 7.38 (d, J = 8.8 Hz, 2H, Ar–H p-Cl C6H4), 7.35–7.30 (m, 1H, Ar–H), 4.63 (s, 2H, S–CH2), 4.10 (q, J = 7.1 Hz, 2H, N–CH2), 1.29 (t, J = 7.1 Hz, 3H, CH3).
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PMC11747949
When analyzing tumor cell fraction by flow cytometry, we observed no changes in CCR2 or PD‐L1 surface expression in tumor cells of mice who received CCR2 antagonists (Figure 3F).
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PMC11792888
We also found that combined treatment (R. junceus venom plus etoposide) did not increase cleaved caspase-3 levels in A549 cells, which is congruent with the absence of p53 activation.
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PMC11583010
Murine anti–hNectin-1 (R1.302), anti–hNectin-2 (R2.477), or anti-hNectin-3 (N3.12) mAbs were previously described (16).
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PMC11731614
These findings suggest a direct communication pathway between the epidermis and the peripheral nervous system, which is altered in our HFD mouse model of PDN.
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PMC9429973
While patients with essential thrombocythemia (ET) have a more favorable outcome, the prognosis of patients with advanced myelofibrosis (MF) is particularly poor with limited therapeutic options.
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PMC9429973
Pathogenic RP gene alterations were detected in 31 patients in 9 different RP genes; homozygous CECR1 mutations were found in 8 separate patients.
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PMC9429973
Variant two and seven are described in gastric and lung cancer respectively.
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PMC9429973
To determine the pharmacokinetics and pharmacodynamics of BTX-A51 Methods: This is an open-label, multi-center, FIH Phase 1 study.
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PMC10988555
Based on previous data on metal complexes internalization several hypotheses can be considered.
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PMC11740399
P<0.05 Fig. 6The anti-tumor activity of 3025@ML via lipid metabolism.
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PMC11502962
We found that MIF/CXCR4 LR was the most common crosstalk between tumor cells and macrophages in G01 and G02 ( Figures 4 – 6 ).
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PMC10698968
Haematoxylin and eosin staining show that MF-R 3 cells growing as myxospheres completely recreate the morphology and architecture of the areas of high cellularity of the patient’s tumour mass (Fig. 4A and B).Fig.
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PMC11489351
Fluorescence intensity from individual cells is shown in a scatter plot (medians shown in red line) from five biological replicates.
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PMC9429973
Median time of follow-up was 2 years (range: 11 days – 16 years).
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PMC9429973
Secondary objectives included progression-free survival (PFS), overall survival (OS), safety and tolerability, and minimal residual disease (MRD)-negativity.
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PMC8358176
The expression of TNF family genes, key players in apoptotic pathways in Jurkat cells, was analyzed, following exposure to two CNTs.
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PMC11519077
In addition, we found that in terms of increasing CDX2 expression, AD-MSC-, AD-CM-, and BM-MSC-treated groups were efficient while the other treated groups were not so.
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PMC11267036
Finally, the cells were enumerated and captured by a microscope (DMi3000 B, Leica, Wetzlar, Germany).
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PMC9429973
Results: We identified 292 cases with pathogenic or likely pathogenic DDX41 mutations with conspicuous ethnic diversity, of which 159 (54%) accompanied somatic mutations as well, while the remaining 53 cases had somatic mutations alone.
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PMC9370419
The Northern blot results showed that the HBV RNA synthesis was influenced by estradiol benzoate, as 3.5 kb RNA, 2.4/2.1 kb RNAs, and 0.7 kb RNA declined in a dose-dependent manner (Figure 3D).
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PMC4695073
The data were analyzed using GraphPad Prism 5 software to obtain the IC50.
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PMC10772747
Regarding the resveratrol compound, out of the 27 target genes, 18 compounds were found to be associated with breast cancer target genes.
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PMC11424574
Notably, Kaplan–Meier analysis demonstrated that higher MTA2 levels correlated with poorer overall survival in human OS patients (Figure 1D; HR, 1.62; P = 0.019).
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PMC11574271
T24 cells were infected with hsa-miR659-3p-expressing lentivirus (termed as T24-659-3p).
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PMC11627060
Many plants contain antioxidant molecules, and natural antioxidants are becoming more and more popular than manufactured ones .
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PMC11705067
The GSE53757 and GSE66272 datasets are the commonly utilized expression profiles for analyzing differentially expressed genes in ccRCC.
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PMC9693627
First, the original start codon was intentionally preserved to avoid alternative translation initiation.
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PMC6379402
These cell lines are commonly used to study breast, prostate, colorectal, and lung cancers, and are not part of the commonly misidentified cell lines.
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PMC11576293
These data demonstrated that compounds 22 and 29 exhibit efficacy as antiproliferative agents by acting as inhibitors for both EGFR and VEGFR-2.
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PMC10538569
High levels of HIV-1 production were observed in co-cultures of iDCs with captured HIV-1 AD8 and CEMss-CCR5-GPI-scFv AB65 ( Figure 3A ).
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PMC9429973
Denosumab, a fully human monoclonal antibody, binds to RANKL to inhibit the formation, function and survival of osteoclasts, the cells responsible for bone resorption.
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PMC6799808
As mTORC1 is also negatively regulated by the AMPK-TSC2/raptor network and positively regulated by Rheb, we also examined whether GLPT suppresses mTORC1 by activating AMPK and inhibiting Rheb.
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PMC11714165
Evaluation of hematological parameters and liver/kidney function in nude mice after administration of different peptides (n = 5).
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PMC11627133
All aptamers used in this experiment exhibited high specificity towards their respective targets as confirmed through multiple negative control experiments, including all aptamers individually (Figure S7B).
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PMC11657744
To examine the localization of AurA in siRNA-depleted cells, we performed immunofluorescent staining for AurA with gamma-tubulin antibody (Fig. 4E-F).
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PMC9429973
By comparing the mutational landscape of each patient to their response to the MYBLOCk screening protocol we can investigate the relationship between molecular data and sensitivity to combination therapy.
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PMC9429973
The patient started a new line of treatment and a few days later, the haemorrhagic symptoms improved and the patient was discharged.
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PMC11502962
The CellChat results showed that the MIF/CXCR4 axis was the main crosstalk type between tumor cells and macrophages.
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PMC3734024
Median fluorescence intensity of surface markers stained as in (D) on freshly-isolated CD19 B cells, B cells stimulated with CD40L, and B cells stimulated with CD40L and IL-5. (
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PMC7090062
e The effects of miR-30a on autophagosome formation shown by confocal laser scanning microscopy in GIST-T1/GIST-882 cells.
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PMC10213179
Cells were cultured in RMPI 1640-GlutaMAX medium supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin and were incubated at 37°C in a humidified atmosphere of 5% CO2 in air.
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PMC9429973
Our data suggest an important regulatory role for this gene in MDS and AML subtypes.
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PMC9429973
Average spleen weights were 196±24.1, 59.7±3.18, and 103±35.4 g, respectively.
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PMC11015054
PADI4 expression level in METTL5‐NC coculture group with HL‐60 cells was significantly higher than METTL5‐sh (Figure 5U,V).
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PMC10297204
No significant difference was observed between ethanol concentrations of 30% and 99.5% (p = 0.28).
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PMC9429973
equally contributing authors.
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PMC11655498
Epstein-Barr virus (EBV) infects more than 90% of the human population worldwide and establishes lifelong infection in hosts by switching between latent and lytic infection.
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PMC11591052
The RevertAid First-Strand cDNA Synthesis Kit (Evrogen, Moscow, Russia) was utilized to initiate reverse transcription with oligo d(T) primer on three micrograms of total RNA.
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PMC10114490
Features with LOG2(FC) > 0 are more abundant in PD diHLC-T. Plotted features were expressed in all samples of at least one group.
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PMC10577955
In addition to, their reported antinociceptive activity , cytotoxic, and colon pathogen adhesion-inhibition properties .
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PMC11803918
As shown in Figure 4G, hybrid exosomes with light irradiation exhibited a significantly stronger anticlonogenic effect than the other groups.
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PMC11777207
To confirm that the comparable DC migration in αPD-1–treated mice was not because of a specific clone of αPD-1, we performed the DC migration assay using another αPD-1 clone (clone 29F.1A12).
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PMC11762280
Although necroptosis key molecule (p-MLKL) was expressed at a low level in keratinocyte-specific Mlkl cKO mice by western blotting assay, pyroptosis key molecule (N-GSDMD) and apoptosis key molecules (cleaved PARP and cleaved caspase-3) remained highly expressed after UVB irradiation (Fig. 5D).
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PMC11703992
B The expression distribution of DDIT4 and ARHGAP15 in GCA.
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PMC5311252
Automatic gain control targets were 1e6 for MS1 and 1e5 for MS2.
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PMC9559528
The positive RPR and TPHA tests, along with the biopsy results, confirmed a diagnosis of syphilis.
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PMC9964635
White, amorphous solid.
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PMC8777939
As shown in Figure 2a–d, Supplementary Figure S3 and Supplementary Figure S4a,b, etravirine impaired cell viability in A2780, A2780-ADR, SKOV3, OVCAR8 cells and colony formation in A2780 and A2780-ADR cells.
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PMC9429973
Image: Summary/Conclusion: Previous Ibrutinib therapy reduced the risk for RT in CLL patients.
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PMC10761571
Then, the wells were emptied and 0.1 mL of dimethyl sulfoxide (DMSO) was added.
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PMC9792775
A-204 was cultured in McCoy’s 5A Media (Modified with Tricine).
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PMC11687167
Liu et al. showed that the immune effects of PCSK9 are associated with oxLDL activation and mature DCs and plaque T cells.
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PMC11244823
Overall, we concluded from these observations that our LIPS antigens met the expected properties of identity and solubility for use in LIPS assays.
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PMC11789597
After antibody staining, cells were resuspended in Fix & Perm Buffer (Standard BioTools) containing Cell‐ID Intercalator‐Ir (125 nM, Standard BioTools) overnight at 4 °C.
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PMC9429973
Methods: The Clinical Practice Research Datalink (CPRD) and the Hospital Episode Statistics (HES) databases in England were used to retrospectively identify linked Hb data among patients with SCD (1 April 2007 to 31 March 2019).
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PMC11696576
We thus hypothesize that cPLA2α may support FAO, which in addition to providing energy protects cells against oxidative stress via generation of NADPH.
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PMC2196094
Both CTMCs and MMCs store very large amounts of the various proteases in their secretory granules.
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PMC11591794
It was observed that there was a significant decrease in cell proliferation inversely to the increase in DOX dose.
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PMC11682525
Following each time interval, a live-dead analysis was conducted utilizing triple staining with PI, FDA, and Hoechst dyes.
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PMC10994876
When the Hippo pathway becomes deactivated, YAP undergoes translocation into the nucleus.
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PMC7369657
Following treatment, the apoptosis ratio of the cells was quantified using the fluorescein isothiocyanate (FITC) Annexin V apoptosis detection kit I (Becton, Dickinson and Company, USA) at 24, 48, and 72 h. The cells were harvested and washed twice with cold phosphate-buffered saline (PBS, Gibco, USA) and then resuspended in 1X binding buffer at 1 × 106 cells/ml.
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PMC11513571
RSV at a multiplicity of infection (MOI) 1:2 was used as a positive control, and unstimulated cells were used as a negative control.
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PMC9847912
Approximately 5% of the Gag proteins in HIV-1 are derived from a second, 160-KDa precursor (Pr160Gag-Pol).
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PMC10818573
Therefore, 15 min was selected as the optimal reaction time.
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PMC8992508
These transporters are constitutively expressed in many tissues playing relevant protective roles by the regulation of the permeability of biological membranes, but they are also overexpressed in malignant tissues.
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