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PMC10887351
In previous studies, after central and peripheral drug administration, β3-AR stimulation reduced the appetite .
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PMC11247842
Information on candidate genes and candidate variants for most identified QTL is still incomplete, as well as knowledge about whether the same QTL segregates across breeds.
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PMC11025029
The corrected version of Fig. 3, now correctly showing the data for the si-TJP3 experiment with the A2780 cell line, is shown on the next page.
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PMC9046263
Differentially expressed HERVs were noted in 39.9% (945/2367), 4.8% (66/1374) and 9.8% (122/1243) of all the unique HERV loci in the A172, M059J, and H4 cell lines, respectively.
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PMC11407042
We have recently identified that the response regulator FleR is a repressor of T3SS in P. aeruginosa.
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PMC9429973
1 year OS was estimated as 58%, 3 year OS as 53% and 3 year DFS as 64%.
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PMC8740518
Malignant mesothelioma (MM) is an uncommon but highly aggressive tumor that is associated with asbestos exposure.
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PMC10495912
The AutoDock V.4.2.6 software was utilized for docking TQ to the SOD and CAT enzymes, after minimization the energy of all molecules.
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PMC6835428
The egg-laying hen model has been underutilized due to the scarcity of chicken-specific experimental reagents and due to the logistical challenges of animal husbandry.
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PMC11026382
Additional ammonium sulfate was then added to the supernatant at 80% saturation (an additional 0.2 g/ml), mixed for 10 min at 4 °C, and centrifuged as above, retaining the pellet.
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PMC11760643
Data was acquired using SoftMax Pro version 7.0 (Molecular Devices).
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PMC9118379
Although variant peptides are usually identified from MS data with a stringent false discovery rate (FDR), FDR control could fail to eliminate dubious results caused by several issues; thus, postexamination to eliminate dubious results is required.
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PMC11751675
These compounds have been observed in several plant families, such as Apiaceae, Magnoliaceae, Lauraceae, Cactaceae, Solanaceae, Araceae and Euphorbiaceae, with a special occurrence in the Asteraceae family.
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PMC9429973
Our patients have a good tolerance to the drugs and few infectious episodes have occurred.
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PMC11679326
Also, researchers proposed to use stage-specific embryonic antigen 1 (SSEA-1/Le) as a tumor-associated carbohydrate epitope for glioblastoma stem cells, as the population of SSEA-1 (Le) cells exhibited high tumorigenic potential, which corresponds to the phenotypic characteristics of glioblastoma stem cells .
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PMC11644761
Notably, pterostilbene is recognized for its potential as an epigenetic modulator, as well as for its dose-dependent toxicity and interaction with DNA.
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PMC11317131
This technology could accelerate gene delivery therapeutics and our directed evolution strategy can easily be adapted to improve novel integrases from nature.
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PMC11468365
proposed an innovative approach known as Shapley Additive Gene Set Enrichment Analysis to identify the genes that enhance the synergy of drug combinations.
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PMC11786767
A: Representative images of mitotic chromosomes in prometaphase before and after expansion (Pre-Ex and Post-Ex, respectively).
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PMC11682525
Flow cytometry assays were employed to examine the cell surface markers expressed by hHFSCs.
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PMC9429973
Results: In ACTIVATE, 40% of pts treated with mitapivat (N=40) achieved a Hb response; in the LTE, pts randomized to PBO in ACTIVATE showed similar improvements in Hb levels after switching to mitapivat.
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PMC11777207
The up-regulation of CCR7 by migratory DCs mediates intravasation through the button-like junctions of CCL21-expressing lymphatic capillaries (17–20).
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PMC5874706
Unloaded cyclodextrin nanoparticles had no cytotoxic effect on A-427 lung cancer cells, while THN7-loaded α-cyclodextrin nanoparticles had a more pronounced cytotoxic effect after 24 and 48 h of exposure compared to THN7 alone.
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PMC10878518
N.S., not significant.
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PMC9429973
M-MDSCs and PMN-MDSCs were sorted from BM samples, RNA was extracted in order to subjected to transcriptomic analysis (RNAseq).
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PMC11680049
Our studies clearly support the idea that transfusion tissue, rich in secondary metabolites with high antioxidant properties, including flavonoids (catechins and proanthocyanidins), performs a protective function for the vessels during collapse/embolism, likely reducing the mortality of leaves (needles), branches, and tree tops during severe water stress.
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PMC11632064
Seahorse XFe96 cell cultures microplates (Agilent Technologies Inc.) were pre-coated with 50 μg/ml Poly-D-Lysine (#A3890401; Thermo Fisher Scientific) in D-PBS (#311-425-CL; Wisent inc.).
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PMC11495567
The treatment media were removed and replaced with CaspaTag FLICA reagent (1:30 dilution) in experimental media.
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PMC11322866
The simultaneous addition of GSK126 and RRM2 overexpression resulted in a further increase in p21 and a further decrease in H3K27me3 level, suggesting that this is a major mechanism underlying cellular senescence.
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PMC9429973
RP2D of QUI was 60 mg in AZA arm and 40 mg in LDAC arm.
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PMC9429973
The most frequent side effects were hematological (anemia 49.3%, neutropenia 42.7%, thrombocytopenia 42.5%) and cardiovascular (hypertension 14.5%, heart failure 2.5%, arrhythmias 3.6%).
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PMC11765988
Cytokines produced by tumor cells (i.e., in Hodgkin or T-cell lymphoma) or cells in their microenvironment can evoke granulomatous inflammation.
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PMC11607321
Due to its inherent limitations, e.g., its inability to account for confounding effects and data structure, a new method, similar to that described by Wainberg et al. based on a generalized linear model (GLM) was tested.
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PMC11787355
f Fluorescence reporter signal of TCR-Jurkat cells upon overnight coculture with various peptide-loaded target cells.
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PMC9429973
for 3 weeks.
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PMC11767582
The best cytotoxic activities were shown by triazole analogues combining a methoxyl group at the para position of the phenyl B-ring with a chlorine atom (3n) or a hydroxyl group (3r) at the meta position.
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PMC11806106
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
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PMC11476837
The results were expressed as a percentage of control cells (100%) incubated in medium only.
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PMC9429973
The INK4 family comprises p16, p15, p18 and p19 which are CDK4/6 specific inhibitors.
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PMC7215832
The required number of cells was centrifuged at 1500× g for 5 min at room temperature and washed in 10 mL of HEPES-buffered saline (HBS) at pH 5.5 or 7.4 at room temperature.
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PMC11641532
These cell lines are well-characterized and commonly used in ccRCC research, but expanding the study to include a broader range of ccRCC cell lines for both knockdown and overexpression experiments would provide a more comprehensive understanding of the diverse roles of HSD3B7 in ccRCC progression.
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PMC6889484
Multiple rounds of infection were monitored up to 42 days post-infection. (
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PMC11592837
Representative images are shown in Supplemental Figures S2 and S3.
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PMC11506827
These findings highlight the potential of GEF to enhance TRAIL-induced apoptosis and DNA fragmentation in RCC cells.
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PMC8270637
T lymphocytes, belonging to one of the most important immune cell types in the HCC microenvironment, play an important role in the aggressiveness and progression of HCC (5).
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PMC11549062
The level of plaque apoptosis was also examined by Tunnel stain, and the results showed that apoptosis was markedly aggravated in vulnerable plaque (Figure 1C,D).
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PMC11301242
Enhancer hijacking, a phenomenon where enhancers are repositioned to new chromosomal locations, has been proven to result in the activation of gene expression [, , ].
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PMC9545474
Complexes 4–8 were synthesised by stirring the 16‐electron complex 3 with an equimolar of the corresponding phosphine ligands in a solvent suitable for the ligand (dichloromethane for PTA, DAPTA, and PPh3; acetone for TPPTS and p‐DANPHOS).
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PMC11347429
For DEGs of ICU patients compared to healthy controls, pathways for ‘Chromosome segregation’, ‘Regulation of viral genome’, ‘Nuclear division’, ‘Response to LPS’, ‘Response to bacterium’, ‘Regulation of inflammatory response’ were up-regulated genes ( Figure 4B ) and ‘Activation of immune response’, ‘Lymphocyte differentiation’, ‘Adaptive immune response’, and ‘Leukocyte cell-cell adhesion’ were down-regulated genes ( Figure 4C ).
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PMC9429973
Infections occurred in 29 patients (63%; grade 3/4 28%).
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PMC9429973
These drugs have been recently introduced in the treatment of chronic lymphocytic leukaemia and acute myeloid leukaemia.
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PMC11637284
The AlphaFold2 model furthermore suggested that only the C-terminal half of the p166 C-tail might be involved in the TAC60 interaction.
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PMC9429973
His MC Tryptase was 54.4µg/L. JAK2 V617F & C-KIT D816V mutations was detected.
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PMC11637276
Plates were centrifuged for a few seconds at 500 rpm, kept at 4°C for 8 min and then polymerised for 2 h and 30 min at room temperature.
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PMC11401358
The ROS scavenging ability of (GAGSGA)2 was tested by flow cytometry.
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PMC11055443
Until now, the world has undergone seven pandemics and Cholera is endemic in more than 50 countries that annually affects 2 to 3 million people in the world.
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PMC5600151
Along with PAMAM, they were also widely studied.
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PMC11752767
Melanoma is one of the most aggressive and deadly types of skin cancer.
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PMC9429973
Median age of patients at diagnosis was 71 years (range, 24-90 years).
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PMC11612794
Next, we examined the mitotic localization of Mis12C in these cells.
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PMC8358006
d The index of H3K27me3 peak signal or peak height (y-axis) plotted against peak breadth (x-axis).
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PMC6163708
It has been nevertheless demonstrated that low-MITF melanoma cell lines can also proliferate very fast, implicating sufficient antiapoptotic protection .
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PMC11694109
Therefore, analysis of the immune microenvironment requires techniques distinguishing the signals from the benign T cells and the neoplastic T cells.
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PMC11774112
As shown in Figure 4A, no significant cell toxicity was observed in A375 or NIH3T3 cells after 72 h of incubation with blank-PHRD (0.1–100 μg/mL).
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PMC11552389
Hallmark pathways exhibiting significant enrichment were detected through 1000x permutation tests.
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PMC10900886
Removal of α (2,3)-linked and α (2,6)-linked SAs on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 µg/mL; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA) (5 µg/mL; EY Laboratories; BA-6802-1), respectively.
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PMC11697189
The location of CNV alteration of HSP genes on 23 chromosomes using TCGA cohort. (
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PMC11680562
These two polymers were chosen for the gel formulation to deliver the CuO NPs and drug 5-Fu.
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PMC11512230
Moreover, many plant viruses are highly susceptible to chemical, thermal, and genetic modifications to produce stable forms .
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PMC9429973
Depletion of the JAK2V617F alle burden, which may lower the risk of progression to myelofibrosis, was observed in ropeginterferon alfa-2b treated patients; JAK2V617F allele burden <1% at 6 years was achieved in 19/92 (20.7%) patients in the ropeginterferon alfa-2b arm with baseline allele burden >10%.
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PMC11286266
Cells were analysed under basal (UT) and ER stress conditions (Tm, 2.5 µg/ml, 20 hr).
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PMC11472569
This study also demonstrated that the functional transfer of ABCB1 occurred across different tumor cell types, suggesting a broad mechanism for the dissemination of MDR.
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PMC9250505
To efficaciously improve PARPi-based treatment, novel targeted combination approaches that produce a more favorable prognosis are required.
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PMC11371747
MSTO is MSTO-211H.
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PMC6627640
Interestingly, decreased H3K27 methylation has been previously associated with increased DNA promoter methylation in pluripotent ES cells which share many similarities with EC cells .
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PMC9429973
P values of these indicators were all less than 0.05.
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PMC11453018
Survival analysis utilized the Survival package (version 3.5) for single Cox models and glmnet (version 4.1) for Lasso Cox models.
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PMC11739484
A375 cells were seeded in 6-well plates and grown to 90% confluence.
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PMC8345486
The IC50 values for compound 23 ranged from 27 ± 3.6 μM (A2780 cells) to 46 ± 5.2 μM (A2780cisKB cells), which were 2–3.5 times lower than that of the unmodified ferra bis(dicarbollide) (A2780, p < 0.01; A2780cis, p < 0.001; A2780cisR, p < 0.05; A2780cisKB, p < 0.05; SKOV-3, p < 0.05; and OVCAR-3, p < 0.01).
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PMC5386478
The evidence that TG2 is activated by reactive oxygen species implies TG2 acts as a stress responder that may confer a growth advantage to cancer cells to survive in micro-environmental hypoxia.
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PMC11575040
Therefore, MMP dissipation may be accompanied by cell growth inhibition and apoptosis, although this causal relationship remains to be elucidated.
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PMC10810426
LAMIN B1 and GAPDH served as controls for nuclear and cytoplasmic fractions, respectively.
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PMC9429973
Image: Summary/Conclusion: Except for ruxolitinib, no treatment has shown superiority over other treatments and no new drugs have been approved either as first-line or second-line treatment for aGVHD in the past 30 years.
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PMC9429973
Additionally, men are at an increased risk for SPMs compared to women.
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PMC9429973
Aims: This single arm study (ChiCTR2000038140) evaluated the efficacy and safety of the combination of zanubrutinib, a novel, highly selective BTK inhibitor, and dexamethasone in patients with WM.
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PMC9429973
The remaining two patients discontinued ibrutinib due to progressive disease.
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PMC11607638
In the present work, we aimed to identify the SK and SKR genes from the genome of A. aegypti, to analyze their anatomical and developmental transcriptional patterns.
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PMC11615743
Owing to the harmlessness of L‐DNA‐based logic devices, isolated cells can be cultured in an artificial medium for downstream applications, such as omics analysis, drug screening, and immunotherapy.
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PMC11740207
AML initiation and transmission.
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PMC11513571
Our results showed that low levels of TSLP were observed after 24 h of culturing A549 cells post stimulation with rSP.
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PMC10820104
The compounds were dissolved in optically pure solvents Uvasol (spectroscopic-grade solvents, Merck, Darmstadt, Germany).
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PMC10858941
On the right, the graphs show the quantification of cellular fluorescence of alive and death cells in control (blue) and NNC-treated devices (orange). *
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PMC11767582
Plastics for cell culture were supplied by Thermo Scientific BioLite.
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PMC11499381
In all cases, statistical significance was accepted when P < 0.05.
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PMC10024132
After expansion, cells were counted using the trypan blue exclusion method.
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PMC9250505
BRCA deficiency/mutation activates the AKT pathway with consequent BAD phosphorylation (64, 65).
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PMC7685376
The combined chemotherapy regimens for HCC are oxaliplatin plus 5-fluorouracil (5Fu), gemcitabine plus oxaliplatin, capecitabine plus oxaliplatin, capecitabine plus cisplatin, and adriamycin plus oxaliplatin.3,4 However, the efficacy of chemotherapy regimens is not ideal, and toxic effects are large.
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PMC7504302
Among others, dysregulation of receptor tyrosine kinase (RTK) signaling results in abnormal activation of the PI3K/Akt/mTOR, and MAPK pathways .
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PMC11024707
In different studies carried out on various tissues, the level of mi-RNA-23a was shown to have changed in autistic subjects in comparison to the controls.
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PMC11707577
Successively, the plate was exposed for 30 min to a visible light lamp (Valex cold white LED, irradiance on the multiwell plate = 24.0 mW cm).
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