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PMC11266100
Based on the results, the 7.5 kV/cm × 300 ns × 250 pulses bursts delivered at 1 MHz using a standard electroporation buffer (No. 1) have been selected as an optimal protocol for long-term transfection to develop a luminescent clone for in vivo research and tumor visualization, which was a success.
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PMC9429973
Median changes from baseline are reported.
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PMC6364197
Since leukemia cells reside in the bone marrow niche, where leukemia cells escape from the chemotherapy drugs (32,33).
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PMC11789597
It has already been demonstrated that glutamine depletion and inhibition of glutamine metabolism inhibit MM cell growth and enhance sensitivity to anti‐MM drugs.
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PMC11512230
Our results demonstrate the specificity of naked SP from AltMV virions for adherence to ES cells, and this effect can be later elevated by further making structural modifications to spherical particles.
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PMC11753435
Before experimental testing, we computationally benchmarked the full PepPrCLIP pipeline via AlphaFold-Multimer, a massively improved method for peptide-protein docking as compared to previous docking strategies (32, 33).
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PMC11609529
Firefly luciferase activity was normalized to Renilla luciferase activity for each sample.
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PMC8345486
Programmed cell death phases, early apoptosis, apoptosis/necrosis, and necrosis were determined in the A2780cis cells in a cross-sectional study of all metallacarborane-modified adenosine derivatives 14–16, 18–20, and 22–24 and unmodified metalla bis(dicarbollides) (compounds 1–3).
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PMC11640115
The CPS was calculated as follows: existing tissue specimens obtained by biopsy or surgery were immunostained with the anti-PD-L1 mouse monoclonal antibody (Clone22C3), and the number of positive cells (tumor cells, lymphocytes, and macrophages) was divided by the total number of viable tumor cells and multiplied by 100.
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PMC2614416
HepG2 cell line, rather than hepatoma, is chosen for proteomic comparison with normal human liver in this work.
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PMC11604768
pcDNA3.1-GPR91 was generated by PCR amplifying the GPR91 CDS from SUCNR1-Tango (Addgene #66507) (introducing a stop codon) and inserting into pcDNA3.1(-)/myc-His A using Gibson assembly.
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PMC11476837
Doxorubicin, a member of the anthracycline family, is among the most widely used chemotherapeutic drugs for treating cancer, including breast, ovary, bladder, lung and thyroid cancers, sarcomas, and blood cancers including acute lymphoblastic leukemia, acute myeloblastic leukemia, and Hodgkin lymphoma .
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PMC11591794
The cell medium was changed every 3–4 days and cells were passaged when they reached 80–90% density.
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PMC10998613
One notable feature of the BiFC assay is the strong binding affinity between VN and VC, resulting in self-assembly of the Venus protein.
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PMC11599565
Nested cells express a vast number of pivotal transcription factors typically observed in embryonic development.
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PMC9429973
CRS occurred in 16 (84.2%) pts (1 grade 4); median time to onset was 8 days (range: 5-11) and all events resolved.
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PMC10605143
The slowdown in the growth of MCF10A cells after HSF1 silencing correlated well with a reduction in the number of cells expressing Ki-67 (a biomarker of proliferation) (Figure 2c).
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PMC9429973
F. Kallel, A. Chalbaoui, O. Kassar, I. Saguem, M. Charfi, I. Aguerbi, Y. Fakhfekh, L. Khalifa, I. Frikha, I. Ben Amor, S. Hdiji, T. Boudawara, M. Ben Hmida, M. Mdhaffar, M. Elloumi Hematology department, Hedi cheker hospital; histology department, Habib bourguiba hospital, sfax, Tunisia Background: The AL amyloidosis is one of the most common types of amyloidosis that is derived from the immunoglobulin light-chain.
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PMC11697065
BisAb1 and BisAb2 contain single chain variable fragment (ScFv1/2) domains that permit bivalent antigen recognition which were removed to create monovalent controls for each bispecific antibody.
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PMC9429973
Aims: Here we report findings from matching-adjusted indirect comparisons (MAICs), conducted to correct for potential imbalances in baseline characteristics, comparing the primary endpoint SVR35 and secondary endpoint TSS50 at Week (Wk) 24 for the combination of pelabresib and ruxo (MANIFEST Arm 3) with Phase 3 ruxo monotherapy (COMFORT-I and II, SIMPLIFY-1) and fedratinib monotherapy (JAKARTA) data.
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PMC10978090
Platelet count and anti-PF4/H IgG level kinetics before and after surgery in patient #1 ( A ) and patient #2 ( B ).
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PMC11783017
Compared with those groups, the tumor growth trends in other groups were obviously attenuated, especially for Mn/NH@PGP and Cis/Mn/NH@PGP groups.
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PMC7081114
A total of 186 HCC tissue specimens and matched adjacent normal tissues collected at Gansu Provincial Hospital between 2013 and 2018 were included in the study.
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PMC10380508
A summary of the clinical information including age at diagnosis, gender, ethnicity, and pathologic stage (T, N or M) is presented in Table 1.
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PMC11481779
The Hi-C Diffusion model that we propose is composed of multiple components (see Fig. 2).
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PMC11707577
After 3 h of incubation the red fluorescence of the oligothiophenes was detected very clearly on the tentacles and less on the body column region, indicating a preferential uptake and accumulation of M13EGFR(TNP) in these regions.
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PMC11767582
When appropriate, phenolic hydroxyls of commercial aldehydes were conveniently alkylated with methyl or ethyl iodide to obtain aldehydes 4a and 4d, respectively, or protected as tert-butyldimethylsilyl ether (OTBS) to obtain aldehydes 4b, 4c, and 4e (see SI for details).
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PMC11703992
Using “TwoSampleMR” package, the MR analysis was performed and odds ratio with 95% confidence intervals of each exposure to GCA outcomes was calculated.
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PMC10706275
The conventional approach to generating recombinant cell lines requires numerous cycles of shielding and validation for the acquired clones.
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PMC11696659
All patients provided written informed consent for the publication of their anonymised case details.
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PMC11621493
Raw data were processed using MS-DIAL (version 5.1.221218) to identify lipids and generate a target list (19).
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PMC9429973
Results: Free dendrimers have their own dose-dependent toxic effects, which is slightly higher for tumor cells, than for PBMCs.
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PMC9739791
In this case, a photoabsorber acts as a photocatalyst that is reduced from its excited singlet or triplet state and then reacts with the Pt(IV) complex.
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PMC11706776
The expression and distribution of SK1 were assessed within the sections.
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PMC11089031
The survival curve of G3BP2 and TM9SF2 in ccRCC was analyzed using GEPIA web tools (G and H).
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PMC11348300
The calibration process involved 10 mice subjected to alternating cycles of breathing a mixture of O2 and isoflurane, followed by breathing CO2.
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PMC11666785
Overall, the benefits offered by corrSMLM over standard SMLM are note-worthy and beneficial for single molecule imaging techniques.
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PMC10380508
Analysis of the online data revealed that PAX2 and PAX8 were expressed at relatively high levels in RCC tumor tissues compared to other PAX genes, while PAX6 was expressed at lower but still significant levels.
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PMC10820104
Compound 1 was isolated as a beige-brown residue.
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PMC11589157
Identified repeats were soft-masked for gene annotation.
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PMC9429973
Results: The rs1001179 SNP genotyping showed that CLL cells harboring the T allele exhibited a significantly higher catalase expression compared with cells bearing the CC genotype.
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PMC11185260
N = 4–5/group. (
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PMC11194678
Representative images showing co-expression of mCherry-HTTexon1 Q25 and PEGFP-C3, EGFP-STAU1 WT, 5KE mutant or HKΔRBD4 mutant in COS7 cells. (
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PMC11678130
Susan et al. observed that 5-FU triggered a concentration-dependent decline in the viability of HaCaT keratinocytes of up to 69% (at 75 µM), but after a longer treatment of 72 h .
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PMC11621493
The enzyme also transfers fatty acids between phospholipids and from phospholipids to acylglycerols.
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PMC11228511
After 48 h of imaging, the cells were fixed and examined by immunofluorescence confocal microscopy.
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PMC9429973
Image: Summary/Conclusion: Altogether, these results indicate the accessibility of IgH locus and the proliferation in CLL patients with high rate and increased diversity of LSR junctions could be increased in Myc dependent manner resulting in shorter survival and pointing on an AID independent mechanism of IgH recombination.
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PMC11394227
A total of 1 × 10 cells were collected, frozen, and thawed repeatedly in liquid nitrogen and a 37 °C water bath, centrifuged at 12,000× g at 4 °C for 10 min; the supernatant was collected, and the total protein concentration was determined by the BCA protein concentration detection method.
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PMC11707577
Comparable effects were obtained by flow cytometry, where i) a clear shift in the fluorescence mode (Figure 3H), ii) an increase in the mean fluorescence intensity (Figure 3I) and iii) a raise in the percentage of fluorescent cells (Figure 3J) were detected only in A431 cells treated with M13EGFR(TNP).
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PMC11700247
A-D) Expression of ifna1, ifnb1, ccl5, and cxcl9 after 24, 48, and 72 hours of RT in vitro. (
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PMC11566417
However, when comparing the potency of the CM of both primary and immortalized MDECs at p10 and p20, both the number of branches and the number of meshes significantly decreased indicating the angiogenic potential of primary and immortal MDECs is lost over time in culture (Fig. 4c(i) and (ii)).
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PMC11354239
The contribution of this mechanism during anti-CD20 therapy remains limited .
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PMC11576293
The cytotoxicity of the most potent compounds was further confirmed by determining their ability to cause apoptosis, as shown by the Bax/Bcl2 ratio.
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PMC11768927
In contrast, the expression of a related protein, MxB, did not impede VSV replication, underscoring the specific role of MxA in conferring resistance to VSV.
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PMC7185206
A PD-L1 protein-to-mRNA ratio was calculated for each tumor sample.
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PMC11726848
To date, the application of high-dose-rate (HDR) sources (300–2400 Gy/h) is common in brachytherapy .
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PMC11247842
We annotated all the significant variants within a 1 Mb flanking area of lead SNPs, which resulted in 11 SNPs (Table 4) with potential effects to alter the coding sequence of genes or regulatory elements that could be considered as potential causal variant candidates.
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PMC11612315
b Mean normalized current amplitude of TRPV1 currents (nA) after treatment with 1 µM exendin 9–39 or 1 µM BCTC compared with the control, pH 5.5 (blue) (mean ± S.E.M.).
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PMC9429973
Dose escalation is ongoing in this Phase 2 study of anemic patients with MDS; updated data from Part 1 with safety, PD and efficacy data from cohorts 4 and 5 will be presented for the first time at the meeting.
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PMC11342785
N here refers to droplet number.
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PMC5311252
Our primary focus was directed towards TSs because inhibition of such targets would promote oncogenic growth.
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PMC11676670
A cytotoxicity assay was performed for the estimation of relative sensitivity of the cell lines OVAR79, SKOV3, and OVCAR3 to known pharmacological agents utilized for ovarian cancer treatment.
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PMC9429973
PET/CT scan showed infra-diaphragmatic hypermetabolic adenopathies (maxSUV 15.1) and splenic involvement.
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PMC11345828
The best improvement in clearance was observed with the (4-tetrahydropyranyl)methyl substituent in both the dimethyl and trimethyl pyrimidine series (22 and 26, respectively).
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PMC11502962
GIST cells can regulate macrophage M2 polarization through the MIF/CXCR4 axis to form an immunosuppressive microenvironment.
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PMC11794568
In recent years, with the deepening of molecular biology research, the important role of mitochondria in tumor development has been gradually recognized.
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PMC11596967
Flow cytometry was employed to analyze the stained cells, and FlowJo software (TreeStar) was used for data analysis.
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PMC11578343
The transit time of cells in each zone was linearly proportional to the zone length (Figure 3E, i) and the frequency of strain experienced by cells in each zone was nearly identical (Figure 3E, ii).
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PMC11461833
Then, the cells were harvested and lysed with lysis buffer (20 mM Tris [pH = 7.5], 150 mM NaCl, 20 mM KCl, 1.5 mM MgCl, 1% glycine, 0.5% Triton X-100) supplemented with a protease inhibitor (PMSF).
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PMC5854676
Figure 3 shows the results of morphological analysis of the nanomaterials using TEM (a-b) and SEM (c-d).
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PMC9587866
Active site residues within the EYA3 protein were predicted by Prank-Web (Jendele et al., 2019).
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PMC9436459
From the five MDAAs kuwanon L (118), sanggenon G (124), sanggenon D (128), sanggenon C (130), and sanggenon B (165), compound 124 was discovered to be a potent antibacterial agent against Streptococcus pneumoniae with a MIC of 5.44 μM .
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PMC8708099
The protective role of osthole was demonstrated in rats with induced ischemia, which caused an increase in plasma pro-inflammatory CKs level and a decrease in anti-inflammatory CKs in the infarct area .
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PMC11754436
Quantitative analysis of apoptosis rate of 143B cells in different groups, *Represent comparison with the NC group. (
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PMC8303372
GA-loaded PLGA NPs were then coated with CS, as reported previously , for the preparation of GA-NPs.
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PMC11687167
The prodomain is in yellow, the catalytic domain is in red, and the CHRD is in blue (Figure from PDB ID: 2PMW).
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PMC11794847
In this study, we identified HVEM as our gene of interest.
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PMC10040136
The transcription of TGF-β2 was significantly higher in the drug-resistant group (H).
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PMC7039683
The multi-layered palate shelf epithelium was manually isolated as described previously (Zhang et al., 2017).
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PMC10808409
A statistically significant P value of less than 0.05 was acceptable.
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PMC11545737
Pathogen-associated molecular patterns (PAMPs) present in bacteria and outer membrane vesicles (OMVs) can trigger pyroptosis pathways in different cell types with different outcomes.
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PMC9848491
A nomogram integrating age, grade and risk score to predict 1-, 3-, and 5-year OS for UCEC patients. (
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PMC11783017
The proteins extracts were prepared using RIPA lysis buffer and their concentrations were measured using the BCA Protein Assay Kit.
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PMC11742670
Even though the number of XAI investigations is rapidly expanding, this paper considered the first to address the use of XAI with Vidarabine Features.
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PMC8609870
RSA seriously endangers women's reproductive health and causes great physical and mental pain for patients and their families.
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PMC10996034
The results revealed that the expression levels of MMP1 and ZYX were significantly increased in A2780/Taxol cells, while UNC5C expression was significantly decreased, which was consistent with the results of the transcriptome sequencing.
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PMC11585587
Cell experiments were then employed to validate the anti-tumor activity and mechanism of RA.
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PMC8956657
E Crystal structure of MERS-CoV ORF4b NLS bound to IMPα2 at 2.1 Å resolution.
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PMC9429973
Summary/Conclusion: DA-EPOCH-R is an effective treatment for aggressive B-cell lymphoma limited by tolerability.
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PMC9429973
During maintenance, 21/32 (66%) patients discontinued therapy, because of progressive disease (14/21; 67%), toxicity (2/21; 10%; infection and intracranial hemorrhage), non-compliance (1/21; 5%), intercurrent death (1/21; 5%) and other reasons (3/21; 14%; physician’s choice, dementia and patient condition).
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PMC9429973
As leukemia did not develop in recipients of cells treated with the antagonist CH-223191, genes differentially regulated in these cells might be critical and specific to LSC function and will be candidate targets for LSC-specific treatments.
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PMC11519567
We observed a significant increase in the number of SS18-SSX condensates, which was ~150% more efficient than SS18 (Fig. 1B, C).
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PMC11727638
Although the mechanisms underlying the differences in CD20 expression levels based on cell type have not been elucidated, the upregulation of CD20 expression on TK cells cultured in the Cell Dome could be due to hypoxia.
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PMC7090062
Therefore, we investigated alternative pathways that might be responsible for KIT-independent IM resistance in GISTs.
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PMC6379402
Furthermore, 39 of our prioritized genes (61.9%) are of unknown biological functions, having no experimentally validated GO-BP annotation.
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PMC9964654
Absolute zeta potential (Zp) values of >30 mV are required for full electrostatic stabilization.
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PMC8524093
Chikungunya virus infection results in a disease known as Chikungunya fever (CHIKF), characterized by high fever, rash, myalgia, headache, and a prominent polyarthralgia (Burt et al., 2017).
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PMC11699465
The Tumors were surgically removed after various administrations for further estimation.
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PMC9848491
The ROC curve and AUCs at one-year, three-years and five-years survival for the predictive signature. (
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PMC7351993
Previous work has shown the ability to sort cells based on fluorescence images or acquire Raman images in continuous flow, but has not shown the ability to acquire and process Raman images rapidly enough to sort cells.
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