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PMC9429973
Recently the biology of Vγ9Vδ2 T cells has recently undergone a new paradigm with the identification of BTN2A1 as the direct ligand for Vγ9 chain of γδ TCR.
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PMC5443223
Rhabdomyosarcoma is the most common soft tissue malignant tumor in infants and children (14).
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PMC4443654
Parameters of mtry=n/3=12 and ntrees=200 were assumed as the algorithm is known to be largely insensitive .
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PMC11437637
In the laparoscopic group, all lymphocyte counts, except for those of NKT cells, decreased after surgery (P < 0.05).
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PMC7612475
Ten hours later, cells were exposed to IR.
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PMC9429973
Copy number variations (CNVs) are detected with ModelSegments software in Tumor-Only mode and common large and focal CNVs in the cohort are highlighted by GISTIC2.0.
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PMC7757104
The downregulation of the OPNc mRNA expression levels was confirmed by RT-qPCR.
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PMC11587541
One possibility is that telomere shortening leads to genomic instability and DNA damage, which, in turn, can contribute to motor neuron degeneration.
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PMC11496728
P < 0.05 and **P < 0.01 compared to the control group.
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PMC11487720
We further elucidated the functional interactions between TRAF2 and WWOX during BCD.
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PMC11765879
Another study investigated the effect of ZSC extract against normal fibroblast cell line HFF-1, resulting in the survival of the cells with almost no reduction in viability .
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PMC7351993
b Scatter plot of differentiated and undifferentiated 3T3-L1 cells in the spatial distribution of intracellular lipid droplets and the lipid intensity per cell area.
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PMC9219615
Any compound which induced >50% cell death at 10 μM was isolated and additionally screened at concentrations ranging from 0.01 to 10 μM to calculate the dose of compound needed to induce 50% cell death.
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PMC9429973
Aims: We hypothesized that FLT3-ITD mutation, a molecular marker of adverse prognosis, is associated with phenotypically and functionally impaired NK cells due to imbalanced expression of inhibitory and activating receptors during AML.
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PMC9727330
The supernatant was discarded, and the pellet was resuspended in PBS for washing and resuspended in PBS with 0.2% formaldehyde for fixation.
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PMC11190062
For the PanCancer pathways panel, a total of 16 genes were significantly upregulated with LPS treatment, and six genes were downregulated ( Figure 2B ; Supplementary Table 3 ).
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PMC11477621
Subsequently, protein interactions with the ligand are monitored throughout the simulation (Figure 8c).
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PMC8973085
Anal.
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PMC11727062
Deer blood extract, specifically deer blood crystals, has been shown to inhibit the proliferation of melanoma cells and induce their apoptosis.
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PMC11261875
Note: 2-Deoxyglucose (2DG) serves as an HK inhibitor; however, its principal action within GIST does not primarily involve the curtailment of energy generation through inhibition of glycolysis.
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PMC11629143
Our data support a causal pathway linking locus‐specific acetylation at the CMV promoter to substantially increased transcription of mAb transgenes, ultimately elevating specific productivity in CHO cells.
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PMC11513571
A comparison of the three stimulants and unstimulated cells (Un) is shown in Figure 3D.
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PMC10919056
The quality of the extracted RNA was assessed by measuring the OD ratio (A260/A280) using a Nano-400A spectrophotometer (Allsheng, Hangzhou, China).
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PMC10440586
CD99 expression in the A-172 cell line of the GSE138535 dataset. (
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PMC6222726
The authors found that cubebin (3) was cytotoxic at high doses (280 μM), but at lower concentrations, no cytotoxic, mutagenic, or proliferative effects were observed for this compound.
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PMC11670407
j Quantitative analysis of OTULIN- and GPX4-positive cells (n = 6).
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PMC11661040
The GO enrichment analysis unveiled the involvement of CGREF1 in various cellular components, biological processes, and molecular functions In order to further verify the effect of CGREF1 on wnt signaling pathway, we activated wnt signaling pathway related proteins in 143B and MG63 cells with stable CGREF1 knockdown, and conducted gene restoration experiments.
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PMC11437637
This study included 112 patients who underwent surgery for uterine fibroids during the same period as the normal endometrial control group.
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PMC11381192
Kaplan–Meier curves of OS and EFS for patients grouped by the nomogram score. (
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PMC11655536
As shown in Fig. 5E, HA inhibited the proliferation of tumors remarkably in the 10 mg group.
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PMC11190538
The C-PC-loaded phytosome was applied as a natural colorant in a highly biologically active, innovative, functional yoghurt.
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PMC11792090
The transmembrane domain of C7R used in this module is a mutant form (p.Thr244-Ile245insCysProThr) that can be activated independent of the presence of IL-7 according to previous studies (36).
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PMC8973085
Found: C, 62.42; H, 4.25; N, 9.90.
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PMC11247842
By comparing it to the AnimalQTLdb , we identify four novel QTL, which are located on BTA1:143 Mb, BTA9, BTA10, and BTA22.
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PMC11604768
Incorporating promoters with desired response profiles in reporter constructs enables precise monitoring of cell signaling and the development of synthetic biology applications where programmable transcription readouts are essential.
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PMC8140214
Moreover, many studies have indicated that the antioxidant capacity of quercetin is stronger than other phenolic compounds (Bai et al., 2013 ▶).
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PMC5673955
Proteins were transferred onto the nitrocellulose membrane in Novex transferring buffer (Invitrogen) at 40 V for 3 h. The membranes were stained with Ponceau S (Sigma‐Aldrich) 25.
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PMC11348300
Fig. 3C presents these data, which were averaged by increments of 2 mmHg on the x-axis.
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PMC11391695
The above results show that the nanomaterials encapsulating HCC cell membranes are better targeted (Fig. 5C).
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PMC9398137
Our results preclinically confirm previous studies that pointed out MCL1 as a critical survival molecule in both DLBCL and Burkitt lymphoma (17, 27).
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PMC9429973
Statistical significance level was 0.05.
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PMC10538569
2.5 × 10 human CD4 T cells per well were activated by mixing with anti-CD3/CD28 antibody-coated beads (Thermo Fisher Scientific) at 1:1 ratio in 500 µl complete RPMI 1640 medium supplemented with human rIL-2 (100 IU/ml) in 48 well plates.
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PMC11764090
Phytohormones play central regulatory roles in plant cold stress responses.
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PMC6461034
Node coordinates are calculated using a Fruchterman–Reingold layout algorithm for force‐directed graph drawing with empirically chosen parameter settings (for N nodes: niter = 100N, area = N, repulse.rad = N, and ncell = N).
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PMC11247842
Among these, chromosomes BTA1, BTA13, BTA20, and BTA29 each had two QTL and BTA19 had four QTL.
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PMC11798926
In response to activity blockade, SynDIG1 might also alter trafficking of cargo, or posttranslational modifications such as palmitoylation could inhibit its interaction with AP2 and cause it to accumulate at the cell surface (Kaur et al., 2016) where it serves a direct role in synapse function.
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PMC11591794
The groups that did not receive any treatment were used as controls.
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PMC11695623
The scatter plot shows the correlation between DCUN1D5 and NDC1, DEPDC1 and CENPA in TCGA-LUAD (Fig. 6E–G).
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PMC10198699
Accordingly, those who sell her cell line for profit enrich themselves at her expense, and she is entitled to claim such profits from them.
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PMC11345828
For matched pairs 29 and 31, we saw virtually no change in the cellular potency; however, with 26 and 32 we observed a modest loss of potency.
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PMC11342785
This result indicates that synMAP circuits can stimulate and stabilize the nucleation of new MTs when sufficiently isolated from other sources of nucleation and polymerization.
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PMC11422497
NC: negative control.
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PMC11659734
Isolate tissues or cells to create a single‐cell suspension for DNA damage study (2) Embed single cells in agarose gels. (
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PMC11720107
Following additional washing, in the final step, 90 µL of substrate solution was added to the wells and incubated for 15 min.
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PMC11206502
The total RNA was extracted from harvested cells using an RNA isolation kit (Nucleo Spin RNA, Macherey-Nagel, Düren, Germany).
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PMC10212663
Chen et al. revealed the enhanced cell migration ability induced by the RANKL/RANK pathway in the KRAS‐mt cell line (A549) in vitro.
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PMC9429973
We further demonstrate that impaired phagocytic capacity and tissue damage are improved by hemopexin-mediated heme scavenging and anti-inflammatory IL-4 treatment in SCD mice through phenotypic and functional macrophage rewiring.
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PMC11506670
Docking simulations show that GDP is only able to bind fully within the binding pocket of wild-type NRAS, but not in the mutants.
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PMC9822769
In particular, OCT3 seems to be the most probable candidate among the transporters considered being the only one upregulated in A2780cisR. Interestingly, MCF7 cells, devoid of any OCT3 expression , show the same low sensitivity to 2 as cisplatin does (Table 2).
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PMC11684821
Ac-6-FP (10 μg/mL) partially blocked MC.7.G5 recognition of A549 WT cells, whereas reactivity of the other TCRs was substantially (MC.27.759S) or totally (K8T-1 and K8T-2) reduced (Figure 5F).
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PMC11632064
MeWo cells were seeded in six-well plates (2 ml/well) at density of 75,000 cells/ml.
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PMC11585254
Data are represented as the mean ± S.D. from at least three independent biological replicates.
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PMC3274243
All three cell types were harvested using 0.2% trypsin solution (all Gibco, Paisley, UK), centrifuged and resuspended at the desired density after a haemocytometer cell count.
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PMC11283030
The top three immunoinhibitors [KDR (b), CD274/PD-L1 (c), PDCD1LG2/PD-L2 (d)] and PDCD1/PD-1 (e) correlation with ACLY expression in ccRCC patients. *
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PMC6684588
For example, even muscle fibers that are histologically identical can exhibit dissimilar responses to disease and injury depending on their developmental origin.
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PMC10777969
These data suggest that FCP23 causes necrosis, while FCP26 instead induces apoptosis through caspase activation.
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PMC9429973
Additionally, there were no statistically differences in hospitalization rate (16% vs 23%, p=0.12) and severity of COVID-19 symptoms (12.6% vs 12%, p=0.87) comparing TFR and TKI treated pts.
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PMC11388384
Next, we asked whether SCUBE1 regulated the transition rates of Hedgehog between discrete diffusive populations by performing two related analyses based on our single-molecule imaging data.
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PMC9532503
The operating parameters used for this purification step are listed in Supplementary Table 1.
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PMC11760643
This mAb can also block human P2X7 activity including ATP‐induced channel activity and pore formation , ATP‐induced IL‐1β release , and P2X7‐mediated phagocytosis .
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PMC7022782
Indocyanine green (ICG), an amphiphilic tricarbocyanine fluorescent dye, is widely used for the detection of tumoral extension in different organs during clinical surgery.
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PMC11264242
After depletion of SS18-SSX1 from Yamato-SS cells, FYN mRNA level was significantly elevated (Supplementary Figure S8).
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PMC11461833
Our results provide new insights for ameliorating the phenotype related to progerin expression, thereby supporting a potential gene therapy strategy.
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PMC9268620
The major component of fractions B and C, separated by TLC in CHCl3, was nordamnacanthal.
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PMC9429973
Summary/Conclusion: The non-statistically significant OS for A±O versus C+O was likely due to treatment crossover.
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PMC8633974
All experiments (GFP or FLuc) were performed in biological triplicate.
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PMC11772449
On the contrary, autophagy can also promote cell survival by inhibiting p62-mediated p38 MAPK pathway activation (Qiang et al., 2013).
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PMC10713411
Relations between various variables and patients’ prognosis were assessed via the analyses of uni- and multi-Cox regression and correlation coefficients between the two groups were computed with the application of Pearson method.
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PMC11569208
Viral titers were determined by high-throughput titration according to published protocol, or standard plaque assay on Vero cells according to published protocol.
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PMC9429973
Image: Summary/Conclusion: In this trial, we proved that early repeated infusion of UC-MSCs could effectively improve the GRFS rate for patients after haplo-HSCT by decreasing the incidence and severity of GVHD without increasing the incidences of relapse for these patients.
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PMC11190238
However, context is critical as cell-type specific EMT regulation needs to be considered.
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PMC11806818
In addition, interactions between some aptamers and nanozymes can either inhibit or enhance the activity of nanozymes when aptamers are modified on nanozymes .
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PMC11718109
C Overexpression of VMAT2-mCherry (magenta) in DA neurons (day 55) immunolabeled with antibody against VGLUT2 (green) show little overlap between these transporters.
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PMC10565698
I. Correlation analysis between miR-211-5p and circUSP10.
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PMC11186948
Five degrees of HDAC2 localization were set (from 0 to 4, where 0 means HDAC2 signal is absent while 4 is strongly present in the nucleus) and number of cells assigned to each degree (HDAC2 + cells) was normalized on the total cell number per image acquired.
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PMC8992508
However, two compounds belonging to the second class, 107 and 108 [Figure 20], behaved as promising inhibitors toward the three transporters, being active in the low micromolar or in the nanomolar range (IC50 on P-gp, MRP1 and BCRP 1.64 M, 0.524 M and 1.39 M respectively for 107 and 1.46 M, 0.501 M and 1.69 M respectively for 108).
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PMC11286266
The biotinylated ATF6α_LD, expressed in mammalian cells, was immobilised on the BLI probe and used as the ligand, and bacterially expressed CRT was used as the analyte.
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PMC11726848
To fit varying tasks and tumor localizations, different sources of ionizing radiation are chosen, such as classic photon and corpuscular radiation sources, sources possessing rare or dense ionizing radiation, and sources with different dose rates .
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PMC10812665
The quantification of glucose and lactate was performed using a colorimetric cuvette assay (GLU-142 and LAC-142, respectively; Diaglobal, Berlin, Germany).
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PMC6650823
To innovate this class of drugs, a wide range of metal-based complexes with ruthenium, rhodium, osmium, iridium, molybdenum, iron, gold, and copper has been designed .
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PMC10818573
A Twist Amp Basic kit was used with the primers from the PCR method, and 50 ng/μL of cellular DNA was used as a template.
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PMC10490530
Semi-quantitative immunoblotting indicated that the hLuz plasmid produced a much higher amount of protein than the Luz-expressing plasmid, despite being under the same promoter control.
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PMC11394227
In the course of cell batch culture, it was observed that the cell viability of CHO-PAb-QS cells was higher than that of CHO-PAb cells, and the cell viability period was extended by two days, showing higher anti-apoptosis.
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PMC9429973
Aims: The goal of this network meta-analysis of randomized controlled trials (RCTs) was to estimate the relative efficacy of zanubrutinib compared with standard frontline treatments for CLL.
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PMC9275501
The results showed that the presence of this system reduced the systemic toxicity of combined treatment, but still maintained the effective cytotoxicity of liver cancer.
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PMC8658661
These can be inherited (Wilson disease, hemochromatosis, and alpha 1-antitrypsin deficiency) or acquired (such as hepatitis and liver cancer) .
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PMC11349530
However, such strategies have generally failed to produce lasting success, especially when it comes to metastatic tumors.
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PMC11679033
As indicated by the value calculated for the combined exposure, RI = 85.74%, the replication of lymphocytes was not significantly affected by CBD and was predominantly disturbed by CIT.
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PMC11335267
for two consecutive days.
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PMC10291556
Glucose consumption by Hela cells treated for 24 h with Pt(IV) prodrugs or free DCF at concentrations corresponding to the IC50 values (Table 1).
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