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PMC2614416
Other work [18-24,27,28] show that, in Hepatocellular carcinoma, the natural free radical scavenger systems of oxidoreductase enzymes that protect cells from oxidative stress, apoptosis and other damaging effects of oxygen free radicals, are strongly compromised.
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PMC8633974
Over 79,000 unique human- or simian immunodeficiency virus (HIV/SIV) sequences spanning eleven global subtypes have been identified .
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PMC11387401
In terms of overlap between RNA interactors, ~24% of identified RNA interactors were found in at least two out of four nRIPseq workflows for their respective HIV-1 protein (NC, MA, Tat, Rev, Gag, Pol), generating a valuable list of potential targets across cell lines and HIV-1 infection status (Fig. 2A).
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PMC9429973
Acute GvHD (aGvHD) is driven by the rapid proliferation of, and cytokine release from, donor-derived T cells.
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PMC10006224
Even if NIPBL-bound cohesin-STAG2 can reach those sites, they would be already occupied by cohesin-STAG1 and STAG2 would not arrest there.
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PMC11487720
MDA-MB-231 and MDA-MB-231 (NH) were considered to come from the exact cell origin.
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PMC11612794
Further structural studies would uncover unsolved mechanisms for the CENP-T-Mis12C interaction.
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PMC11752740
In this experimental study, we utilized the breast cancer cell line SK-BR3 and the normal cell line MCF10A, both sourced from the Pasteur Institute of Iran in Tehran.
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PMC10957991
Analyses of cell–cell junction from confluent 184A1 or MCF10A cell monolayers was performed with Junction Mapper-tool as in Brezovkjakova et al., 2019.
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PMC8557138
Along with sprouting and intussusceptive types of angiogenesis, vasculogenic mimicry (VM) and vasculogenesis enable the fulfillment of the oxygen demand and nutrient requirements of the tumor.
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PMC9878562
VAL-ko migration in the presence of VAL-wt is PTX sensitive. (
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PMC9429973
Although a single injection of recombinant mouse thrombopoietin (TPO) only increased the number of LT-HSC and not of MPP1, the Evi1high cell fraction was enlarged in both compartments (LT-HSC: 23 vs. 50%; MPP1: 22 vs. 47%; n=29, p<0.0001) supported by high-resolution imaging.
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PMC11794588
For brine shrimp assay cysts of Artemia salina were hatched in two-chambered containers with small holes in the partitioning wall.
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PMC11725127
Using GA as a chemical probe, we unveil USP2 as a direct DUB of KRAS in MM cells for the first time.
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PMC11727062
Importantly, it was found to lack significant adverse effects on the animals and to offer protection against cisplatin-induced nephrotoxicity (Meng et al. 2019; Li et al. 2020; Zhang et al. 2021).
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PMC9429973
Median and range were used to summarize continuous non-parametric data.
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PMC11482552
Using the “Limit Event” function, 1,000, 10,000 and 100,000 cells were sorted in triplicate (Total number = 9 Eppendorf tubes) according to the gate strategy shown in Figure 2.
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PMC10006224
Thus, our results are consistent with previous data showing that significant amounts of cohesin can still be found on chromatin after knock down of NIPBL or MAU2.
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PMC10887351
In cardiac muscle, β2- and β3-ARs are coupled to Gi, whose triggering caused an increase in NO through endothelial nitric oxide synthase (eNOS) activity, which is expressed both in endothelial cells and in the ventricular cardiomyocytes , which increased the NO production.
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PMC11519077
In fact, transplanted MSCs support the effective elimination of harmful bacteria and restore altered gut microbiota .
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PMC11544122
P values are indicated for each comparison group.
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PMC11680562
The dishes were incubated at 37 ± 0.5 °C for 24 h after moderate agitating for 1 h to facilitate diffusion.
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PMC9878562
Chemokine scavenging and receptor internalization requires the intact C-terminus of ACKR3 (6).
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PMC11708541
In particular, it was discovered that 3-methyl4-aminopyridine (3Me4-AP) was roughly 7 times more effective than 4-AP (10) and 3-fluoromethyl-4-aminopyridine (3F4-AP) (41a); 3-methyl-4-aminopyridine (3Me4-AP), 3-methoxy-4-aminopyridine (3MeO4AP) (41b and 41c) was about 3–4 times less potent than 4-AP; and 2-trifluoromethyl-4-aminopyridine (2CF34-AP) (41d, 41e) was approximately 60-fold less active.
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PMC11033180
To assess the activity of full-length Sema5A on enteric neuron development and allow for cell-cell contact between enteric neurons and COS cells, gut dissociated cells were plated in a culture well containing transfected COS cells (400,000 cells/well).
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PMC8345486
In contrast to supplementation with compounds 25 or 23, culture of the cells in the presence of compound 2 induced drug resistance (“+2 cells”, Figure 9A,B), which indicates that the cells overcame the relatively proapoptotic action of compound 2 and triggered some adaptive mechanisms.
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PMC10568263
First, 50 mg MnO2 nanoflowers were dispersed in 50 mL of DI water and transferred to a 100 mL round-bottom flask.
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PMC10565698
C. Tumor weight statistics in shNC and sh-circ groups.
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PMC11746942
Our findings demonstrated that the protective role of FTY720 in psoriasis was mediated by internalized S1PR3, highlighting the significance of S1PR3 in the pathogenesis of psoriasis.
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PMC11473843
Given that FAK is overexpressed in multiple primary solid tumors, and even more in invasive and metastatic cancers (Sulzmaier et al, 2014; Weiner et al, 1993), understanding the mechanisms of FAK inhibitor resistance is of paramount clinical importance.
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PMC11626627
3The circATP2B1/miR-204–3p/TWIST1 axis is involved in the regulation of EMT in ccRCC. (
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PMC11637276
Our screen identified MAPK signalling as an important regulator of ECM internalisation.
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PMC8316344
The primer sequences are available in Supplementary Table S3.
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PMC6379402
Furthermore, this makes the remaining one of the prioritized genes, NUDT8 (a mitochondrial Nudix Hydrolase), a good candidate for further investigation.
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PMC10490530
Thus, considering the pool of CBP/p300 protein in cells at any given condition is constant, both p53 and NF-κB may have to compete to form complexes with CBP/p300 or one of the component proteins as a pre-requisite to exert their respective transcriptional regulation activity .
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PMC9429973
This study support performing ASCT for treatment of hematological malignancies despite the COVID-19 pandemic.
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PMC11678368
For triple-negative breast cancer (MDA-MB-231), the complexes exhibited better activity than cisplatin, suggesting a potential advantage in treating resistant cancers.
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PMC9429973
We show that exposure to heme and iron excess drives defective efferocytosis of apoptotic neutrophils in vitro, in cultured bone-marrow-derived macrophages, and in vivo, in mouse model of heme overload.
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PMC11402217
Representative gene FPKM values (D) and qPCR validation (E).
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PMC5963610
Interestingly, as in the 3LL-D122 model, while the individual therapies do not increase survival, in the combination group 40% of the mice remained alive (but not tumor free) after all mice in the control group are died.
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PMC9429973
These cases showed a strong nuclear positivity for cyclin D1 in the DLBCL, but no expression of CD5 and SOX11 was detected.
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PMC8303372
In the first condition the NPs remain intact and, consequently, the GA determined by HPLC is only the quantity originally released into the cytoplasm by NPs.
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PMC11474209
Proto-oncogene expression is controlled by MAPKs by PP2A and is regulated by MC which controls the gene transcription associated with cell development and differentiation (Fig. 1).
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PMC10978090
Platelet count normalized at D20.
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PMC11680049
This suggests that the phenolic complex in the explant of Sequoia sempervirens is dominated by flavonoid compounds, particularly evident in the meristematic tissues.
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PMC11650848
Despite the limitations discussed, we have shown here that the promoter region of the ZFP57 gene is controlled by the newly identified mQTL that regulates allele-specific methylation patterns.
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PMC9429973
Thirty-six patients were on active treatment, whereas 11 had completed their therapies within 6 months from nMoAbs administration, for 4 patients data were missing.
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PMC11474209
The drugs or chemicals are capable of being selectively directed into the mitochondria using a variety of strategies .
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PMC11711066
Particularly, the estrogenic effects of calycosin, which are found in Astragali, such as As‐IV, have attracted attention recently.
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PMC11680049
Extracts were obtained from 1 g of raw biomass of third-passage callus tissue of S. sempervirens.
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PMC11352746
This fluorescent dye was also tested alone to ensure the reagent was not contributing to the apoptosis measured .
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PMC9429973
Aims: To assess the impact of the universal use of intravenous midazolam premedication for the BMAB procedure compared to the use of local anaesthesia only and to determine the patient- and procedure-related factors associated with the patient-reported pain.
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PMC11350568
This system represents significant advancement in the field of molecular medicine, offering a new avenue for targeted therapeutic interventions and the exploration of cellular mechanisms.
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PMC11291697
Top enriched pathways, i.e., those prioritised (p < 0.05) by at least two tools, were considered as potential candidates and underwent more careful evaluation.
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PMC9512971
The results of the PK/PD simulations also suggested an advantage of increasing the dosing frequency rather than increasing the dose per administration.
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PMC8481254
Cell proliferation and colony-formation capacity were measured with CCK-8 cytotoxicity tests (B) and soft agar colony-formation assays (C), respectively, in Bel-7402 and Bel-7404 cells subjected to different treatments as indicated.
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PMC10772747
With an average number of neighbors of 2,291 and characteristic path length of 1835, this network has a density of 0.021.
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PMC10170482
However, there is emerging evidence that cancer cells possess a heterogeneous and flexible metabolic profile.
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PMC11441402
The automated cell imaging system relies on fluorescence microscopy and direct read outs of cell numbers based on the detection of Hoechst-positive and SYTOX green-positive cells.
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PMC6034753
SDS-PAGE-resolved immunoprecipitates and were subjected to Western blot analysis with HA-tagged antibody.
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PMC11593084
However, the limitation of the ideal target antigens is a severe problem.
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PMC11774747
This review provides a comprehensive overview of CAR-VST development, emphasizing strategies to mitigate immunogenicity, such as using a specialized TCR, and approaches to improve therapeutic persistence against host immune responses.
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PMC11806818
In this study, a GPC3 antibody was used as a capture probe, and a GPC3 aptamer modified on the surface of H-rGO-Pt@Pd NPs was used as a recognition probe.
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PMC11740573
However, to complete the picture of calcitriol’s immunomodulatory properties, its ability to stimulate the production of anti-inflammatory cytokines has to be also verified in future studies.
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PMC11087766
The supernatant was collected and serially diluted until individual plaques could be obtained and purified by the above method.
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PMC9429973
Image: Summary/Conclusion: Despite all patients with DLBCL observed being SCT eligible at their first relapse, only half of these patients went on to receive an SCT.
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PMC11489351
In contrast, low pHi favors decreased PDK1 expression, PDH activation, and high pyruvate conversion into AcCoA for entry into the Krebs cycle during cellular respiration.
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PMC9429973
D. H. Kim, F. Jia, C. Y. Ok Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, United States of America Background: KIT p.D816 mutation is strongly associated with systemic mastocytosis (SM).
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PMC10818062
Phosphatidylserine (PS) is a quantitively minor class of phospholipids that contributes to cellular membranes (1).
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PMC3888431
The resulting 59 mappings (Cufflinks, Trinity, and 57×Oases) were merged to a single mapping with the cuffmerge tool provided by the Cufflinks distribution.
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PMC2196094
When comparing MMCs of the two species, both similarities and also certain important differences are found.
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PMC11612794
The experiments were repeated three times, and error bars show the mean of the three replicates ±SD; p-values were calculated by one-way ANOVA (F (3,8) = 17.76; p = 0.0007) followed by Tukey’s test.
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PMC11361748
Isolated cells were stained following Maxpar Cell Surface Staining with Fresh Fix protocol (Standard BioTools).
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PMC11477621
C NMR (150 MHz, CDCl3) δ 188.5, 167.5, 144.5, 140.9, 133.7, 130.6, 130.4, 128.5 × 2, 127.5 × 2, 120.6, 117.8, 108.9, 52.5, 51.8, 27.4.
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PMC9429973
Bone plasmacytomas in most cases are composed of mature plasma cells, while immature morphology is mainly characteristic for extramedullary plasmacytomas.
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PMC11759543
These variations and other unidentified distinctions may be the results of our observations in this study.
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PMC6889484
Western blot analysis of cell lysates and virions showing expression and encapsidation of A3G(DK)P2A and/or A33G(DK)P2A. (
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PMC8469018
Leflunomide is metabolized to one major metabolite A771726 (teriflunomide), which is responsible for its activity in vivo, and many other minor metabolites (Figure 1) .
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PMC11540664
The reduction or discontinuation of treatment was determined by the clinician, depending on the disease status and adverse effects.
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PMC9429973
In multivariable analysis, including the aforementioned variables, presence of ASXL1 mutation (83% vs 53%; OR 4.5) and absence of TP53 (67% vs 32%; OR 3.3) and FLT3-ITD mutations predicted favorable response (61% vs 30%; OR 6.4).
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PMC11787355
g and l were analyzed with Spearman correlation.
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PMC11715644
For +ErbB2 cells, while persistence was consistently higher in entering wide channels, order parameter for narrow channels increased over time, indicating a tendency of 10A + ErbB2 cells to expand regardless of confinement dimension.
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PMC9926039
Moreover, the growth of human lung cancer cells is reported to have been inhibited by methadone, in both in vitro and in vivo experiments.
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PMC11723947
Treg can directly interfere with T cell programming by producing immunosuppressive cytokines.
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PMC11422497
Pearson’s correlation analysis showing a negative correlation between KIT mRNA and miR-494-3p expression in GISTs tissues.
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PMC10938336
These properties were evaluated for a set of active compounds known to be associated with monocarbonyl curcuminoids.
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PMC11637820
CAP is a non-chemical therapeutic strategy (directly deposits RONS), and the major factors that play a key role in the reduced sensitivity in gram-negative bacteria are higher glutathione content, high lipid content, presence of periplasmic superoxide dismutase (SOD), and enhanced DNA repair mechanisms .
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PMC9454852
Following ER homeostasis loss, UPR activation starts: oGRP78 firstly increases and then decreases in a time-dependent manner;oGRP78 translocates to the plasma membrane in a time-dependent manner immediately after the treatment;oXBP1 and CHOP increase in a time-dependent manner.
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PMC11506670
To wit, SW480 has a KRAS G12V mutation; SW948 has KRAS Q61L and PIK3CA E542K; DLD-1 has G13D; HT29 has BRAF V600E; and HCT116 has KRAS G13D and PIK3CA H1047R .
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PMC11612794
Cells were treated with IAA for 12 h, fixed, and stained with an anti-CENP-A antibody.
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PMC9895440
Notably, several studies performed genome-wide CRISPR screens in human T cells using a Cas9 electroporation-based protocol that was shown to enhance gRNA vector transduction rates.
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PMC2196094
Attempts were made to identify additional bands by NH2terminal aa sequence analysis.
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PMC9436459
Finally, ring-closing metathesis of compound S138 using Grubbs II as the catalyst in toluene led to the formation of the desired palodesangren D dimethyl ether (141a).Scheme 14Synthesis of palodesangrens B and D methyl ethers (139a and 141a) by the Ploypradith group Synthesis of palodesangrens B and D methyl ethers (139a and 141a) by the Ploypradith group Similarly, palodesangren B trimethyl ether (139a) could be readily synthesized from aryldiene S123 and known chalcone dienophile S125 by using the same reaction sequences as 141a.
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PMC11786767
RO-3306 not only resulted in a reduction in total chromosome area (Fig. 8E, left) but also altered microtubule organization.
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PMC11792090
Cells co-cultured with E066 CAR-T cells exhibited a 5% reduction in CD70 positivity, which was notably lower than the reduction observed in cells co-cultured with P8F8 CAR-T cells ( Figure 1H ).
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PMC9429973
Primary endpoint was overall survival (OS); key secondary efficacy endpoints were event-free survival (EFS) and complete remission (CR).
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PMC11806182
Troglitazone, known for its impact on metabolic pathways , was found to down-regulate artery development while up-regulating cholesterol metabolism.
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PMC10978090
Surgical treatment of interventricular communication led to four TPE sessions with 100% FFP from D12 to D16.
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PMC11703896
In the previous studies, the use of High-Performance Liquid Chromatography (HPLC) unveiled a retention time of 17.026 min for rapamycin.
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PMC10529414
Previous reports on breast cancer have demonstrated that TALAM1 contributes to the stability of MALAT1, promoting the 3′ cleavage and maturation of MALAT1 and decreasing the migration and invasion of cancer cells .
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