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PMC11544122
However, despite the established role of β-arrestins as endocytic adaptors, there is evidence that some GPCRs use an alternative mode of endocytosis, independent of β-arrestins.
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PMC11676674
For instance, punicalagin is an ellagitannin, previously described as the most abundant secondary metabolite in pomegranate juice.
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PMC11300639
Overexpression of RNF26 decreased the protein level but not the mRNA level of TSC1 in ccRCC cells (Fig. 6c, d), and this effect could be attenuated by a proteasome inhibitor (MG132) but not a lysosomal inhibitor (BafA1) (Fig. 6e, f).
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PMC9895440
d Human cytokines measured by Legendplex (Biolegend) in the serum of Raji-bearing NSG mice treated with anti-CD19-28z CAR T cells with and without SNX9 KO.
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PMC11762280
A Photos of keratinocyte-specific Mlkl cKO mice (Krt14-Mlkl) and control mice (Krt14-Mlkl) at 48 h after exposure or non-exposure to 430 mJ/cm UVB irradiation (n = 5).
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PMC11637276
Of note, both PPP2R1A knockdown (S3D and S3E Fig) and okadaic acid treatment (S3F and S3G Fig) resulted in cell rounding but not detachment from CDM of either cell line, suggesting that the effect observed was PPP2R1A-specific.
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PMC11738017
Multivariate analysis that included EMD and sBCMA to determine the effect on PFS patient outcome.
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PMC11684297
DCFH-DA was purchased from Sigma Aldrich.
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PMC11394227
The binding of puromycin to ribosomes prevents the peptide bond elongation, while tunicamycin inhibits the normal glycosylation of proteins, both resulting in ER stress.
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PMC10571053
Significance was determined by a one-way ANOVA followed by a Dunnett test for multiple comparisons.
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PMC11301242
AML, acute myeloid leukemia; B-ALL: B-cell acute lymphoblastic leukemia; BL: burkitt lymphoma; DLBCL: diffuse large B cell lymphoma; FL: follicular lymphoma; MCL: mantle cell lymphoma; CLL: chronic lymphocytic Leukemia; CML: chronic myeloid leukemia; MM: multiple myeloma; T-ALL: T-cell acute lymphoblastic leukemia; TCL: T-cell lymphoma; WBC: white blood cell; PBMC: peripheral blood mononuclear cell; MSC: mesenchymal stem cell.
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PMC11045125
For Burkitt cell infection with retroviral vectors, the retroviral vectors were co-transfected into 293FT cells with the pMD2.G and pCMV-GAG-Pol vectors.
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PMC11790599
Ultimately, the displacement of much of the cytoplasmic space by the accumulated vacuoles is accompanied by the rupture of the cancer cells (Figures 2A–C).
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PMC11755467
Both CEM-SS and MT-4 cell lines have been described as suitable model systems for monitoring HIV-1 infection in vitro as well as being already tested for their response for treatment with fluoroquinolones on HIV-1 .
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PMC11186948
Mutations in HDAC4 gene are causative of Neurodevelopmental disorder with central hypotonia and dysmorphic facies (OMIM #619797).
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PMC11564322
The mRNA expression was normalized to the GAPDH gene.
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PMC11496728
The above data were from three independent experiments, and the difference was considered statistically significant when P < 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with CHO-WT.
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PMC6268318
These results contribute to the validation of the popular use of this plant species in the treatment of bacterial, fungal, protozoan and viral infections and cardiovascular problems, among others.
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PMC7022782
Moreover, further studies are mandatory for defining the specific uptake routes, using inhibitors of the different steps involved in internalization pathways .
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PMC11788920
When the differentiated Ramos cells were ∼70% confluent, they were transfected with the CRISPR–dCas9 (RNP) complexes using TransIT-X2 transfection reagents (Mirus Bio, USA).
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PMC11087055
While these models suggest that the cell flow pattern is dependent on an intact embryonic architecture, it remains unclear as to involvement of other elements such as signaling pathways and gene expression, which can be also molecularly and cell-biologically changed as a result of surgeries.
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PMC10747139
The cell-colony, wound-healing, and cell-cycle distribution demonstrated that the complexes inhibited the cell growth at the G2/M phase.
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PMC11021472
These results showed that circZDHHC11 is important to maintain BL cell growth.
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PMC11754436
Grouping and treatment of nude mice.
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PMC11085211
Interestingly, the adjacent, weakly active subfraction—2A-33—also contained gallic acid, although at a lower concentration compared to the active cluster, supporting the involvement of this molecule in the weaker biological activity.
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PMC10587429
Place 3–4-week-old NSG-SGM3 mice into a sterile, filtered, ventilated device for housing during irradiation.
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PMC9429973
Image: Summary/Conclusion: MYLOX-1 is designed to explore the safety and clinical effects of GB2064, a novel small-molecule LOXL-2 inhibitor, addressing bone marrow fibrosis as a main element of myelofibrosis, with the aim of decreasing extramedullary haematopoesis and improving haematological parameters, symptom burden and the quality of life for patients with MF.
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PMC11626627
In our research, we identified a positive feedback regulatory relationship between ERβ and TWIST1, which provides new insights into ccRCC.
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PMC5839394
Each result was the mean value of three different experiments performed in triplicate.
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PMC10772747
The activation of CDK 4/6 and E2F, as well as DNA replication, contribute to the transition of cells from the G1 phase (gap 1) to the S phase, known as Cell cycle progression.
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PMC11707577
For the spectroscopic characterization of M13EGFR(TNP), absorption spectra were recorded using a Cary60 UV–vis spectrophotometer (Agilent).
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PMC11225860
This is a result of cells failing to progress through G2 due to the Aurkin A treatment.
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PMC8524093
The symptoms usually disappear between the first- and second-week post-infection, occurring together with the restoration of serological parameters.
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PMC11277157
The esiRNA (Mission system, Sigma-Aldrich, Burlington, MA, USA) consists of a pool of hundreds of siRNA (21 bp each), where each individual dsRNA has a low concentration in the pool, which diminishes most off-target effects, while producing an efficient knockdown.
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PMC11397654
The isatin-bearing derivatives were more active in comparison to the compound 43 which, however, exhibited some cytotoxicity due to the presence of 1,2,3-triazole moiety.
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PMC10988808
D Representative fluorescence images of Xenograft tumor labeled by DAPI, CCDC25, ITGA3.
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PMC11085211
All clusters significantly reduced the viability of both GIST-T1 and PBMCs.
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PMC9318744
SARS-CoV-2 S pseudotyped virus activity was determined using the Bright-Glo Luciferase Assay System (Promega, Madison, WI, USA).
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PMC9429973
Early loss of response in patients who achieved CR after 2 courses of treatment may be a prognostic clue.
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PMC8358176
TNF expression showed a meaningfully elevation in MWCNT-treated cells by a fold change of 5.37 ± 0.41 compared to the SWCNT-treated cells (6.38 ± 0.42; p = 0.021) and control cells (1.03 ± 0.14; p = 0.031).
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PMC11596967
The promising results with Ddes-P3 underscore the potential of mitochondria-targeted antioxidant peptides in radioprotection.
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PMC10873328
The protein expression level of TGFBI after knocking down by siRNA in A2780/CDDP. (
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PMC10747139
Complex 50 showed a promising cytotoxic effect on all the tested cell lines in both 2D and 3D cell cultures.
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PMC7802142
Scale bar, 50 μm.
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PMC11093197
These data suggest that the attenuation of RAS activity is a consequence of RBL2/p130 induction, which acts as a counteractive factor , leading to quiescence to impede cellular transformation.
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PMC11711127
Anergy is commonly defined within two frameworks, either: in vitro, sometimes referred to as ‘clonal anergy,’ wherein anergy accompanies some retained effector cellular function; or that induced in vivo, termed ‘adaptive tolerance,’ and associated with a complete loss of effector functionality (41).
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PMC7582629
All measurements were performed in an automated fashion with a developed data acquisition method.
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PMC10976516
Study is ongoing.
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PMC11352666
Receptor tyrosine kinase inhibitors (TKIs) have shown promise in EWS and other bone sarcomas .
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PMC11453018
To achieve constitutive LMNB1 overexpression, SKES1 cells were transduced with a lentiviral vector carrying LMNB1, while P15 was knocked down using siRNA (Supplementary Fig. 9A).
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PMC7057940
The in-depth study of the molecular mechanisms that regulate the expression of aberrant transcription factors such as EWS-FLI1 is fundamental and necessary (17), because this knowledge will allow us to understand and develop novel therapeutic approaches to this pathology, such as epi-drugs.
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PMC11795610
p < 0.01 versus NOR group; p < 0.05 versus High Ca group.
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PMC11344246
Three targets (TRIP13, CDC20, CDK1) were validated using known small molecule inhibitors.
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PMC11723947
To avoid the side effects that could arise from the ubiquitous expression and pleiotropic functions of HP1 proteins, it might also be possible to inhibit HP1α expression not in vivo, but ex vivo to enhance the efficacy of CAR T cell-based therapies.
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PMC11679033
In contrast, the proportions of M2, M3 and M4 cells significantly decreased compared to the negative and solvent control (Pearson’s χ test, p < 0.05).
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PMC11269792
Next, 20 µL of assay buffer was added to and 100 mL of back-extraction buffer was then supplemented.
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PMC9516401
All the data were examined in triplicate against untreated control cells and collected from three ‎independent experiments.
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PMC9429973
Methods: We performed a retrospective study to assess the response to prednison (dose 0.5-1 mg/kg/day with taper) in combination with eltrombopag (dose 25 mg/day) in 11 patients with newly diagnosed ITP (age ≥ 17 years, the median age was 45.2 years, nine patients (81.8%) were women).
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PMC11742431
The western‐blot results also showed the same trend, which indicated that the degradation level of Survivin protein was positively correlated with the treatment time and treatment concentration of KVN or K14N.
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PMC11772449
We used this database to analyze the expression of MAPK family (p38α, p38β, p38γ, p38δ) in hepatocellular carcinoma and its correlation with patient prognosis] Next, we explored whether p38γhas a regulatory effect on the function of Sora.
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PMC5673955
Jurkat and CD4 T cells were co‐incubated for 72 h with various concentrations of RrA. Cell viability was determined using LDH assay.
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PMC9429973
Summary/Conclusion: Mutations of TNFRSF13B gene do not seem to participate in the pathogenesis and phenotype of CLL patients.
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PMC11411131
Fig. 4Evaluation of various factors affecting Sec insertion. (
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PMC9509578
Their findings indicate that the transcriptional downregulation of the MDR1 gene further aids in the reversal of MDR .
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PMC11796104
Validation experiments were performed on selected circRNAs and related miRNAs in this ceRNA network using peripheral blood clinical samples.
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PMC5363531
In all MPM cell lines analyzed, the subcellular distribution of both TS and pSRC was detected by confocal microscopy after immunostaining with anti-TS (FITC-green, left panel) and anti-pSRC (FITC-green, right panel).
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PMC9429973
Damage to the skeleton bones during primary diagnosis (including SP) was detected in 37.4% (68) of cases.
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PMC8740518
We observed 5-dyhydrocortisol and hydrocortisone levels to be significantly decreased.
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PMC9429973
We also found allogeneic CAR-T cells manufactured from CB could prepare patients for later subsequent autologous CAR-T therapy.
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PMC11552389
The GEPIA database (http://gepia.cancer-pku.cn/detail.php) is an online resource that allows for the analyses of RNA-seq data pertaining to 9736 tumor and 8587 control samples included in the Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) databases and Xiantao tools (https://www.xiantaozi.com)22.
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PMC7582629
The proposed device was able to compensate for the limitations of the two techniques alone.
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PMC11607764
Expression profile of PTBP1 in different cancers and pathological stages. (
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PMC9429973
Image: Summary/Conclusion: A review of the literature showed that 16% of patients with anti-VF inhibitors had an underlying malignant disease, including haematological diseases such as multiple myeloma, myeloproliferative neoplasms and mantle lymphoma.
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PMC11001582
n mice = 5 WT, 6 (GR)400 and 6 (PR)400; n muscles = 8 WT, 12 (GR)400 and 9 (PR)400; one-way ANOVA, Bonferroni’s multiple comparison.
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PMC10421378
Samples stored at −4 °C had a shelf-life of 6–9 months, while also being easier to store, potentially making this a better choice for commercial purposes.
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PMC9429973
All the patients were treated with decitabine supplemented with low-dose chemotherapy with a interval of 4 weeks, bridging to HSCT after three~four courses.
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PMC4360730
Because ontologies need to be adjusted to accurately reflect new and developing scientific results as well as areas of science that are being actively investigated, making metadata annotations often involves interacting with different ontology development groups.
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PMC11534377
For cDNA synthesis we used High-capacity cDNA Reverse Transcription Kit (Applied biosystems, USA).
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PMC11033180
Quantifications in the ENS-COS cell coculture were conducted in the vicinity of the Matrigel dot, within a range covering 0 to 900 μm from the Matrigel’s periphery, utilizing the Axio Observer Zeiss microscope and Zen software (Zeiss, Germany), with a x10 objective.
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PMC8540692
In this regard, 5 µM CCM significantly reduced the number of living cells of A-172 cell line (p = 0.0003), and T98G (p = 0.0430), having no impact on U-138 MG cell line.
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PMC4659567
Elemental analysis (C, H, N) was performed using a Flash 2000 CHNS Elemental Analyzer (Thermo Scientific).
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PMC6642070
As a transcription factor, p53 represses gene expression through binding to the DNA motif consisting of four canonical p53-binding sites arranged head to tail (p53RRE) .
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PMC11411131
Then, Krab-Sec/Luc, a Krab transcriptional suppressor fused with Sec, was designed and exhibited increased signaling upon exposure to MeHg.
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PMC9874064
In order to explore the role of USP1 in DNA damage response in liver cancer patients, the difference of USP1, FANCD2, and BRCA1 gene expression between liver cancer patients and healthy people was analyzed by the Cancer Genome Atlas (TCGA).
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PMC10899471
B, C) qPCR screening of the predicted 9 miRNAs in control and sarcoma tissues from sarcoma patients (B) and HSF and HT-1080 cells (C). (
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PMC9429973
In addition, we implemented novel, non-routinely used methods for the evaluation of the functional status of the spleen in those patients.
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PMC9429973
Providing patients with accurate information about prognosis is important for them to consider how to spend their remaining life.
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PMC8633974
Data are presented as mean ± standard error of the mean (SEM) or standard deviation (SD).
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PMC11707832
Among these, missense mutations stand out as the most prevalent variants .
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PMC10094712
On the other hand, when the same cancer cell line was treated with hSELENOM-KD, an increase in the expression of the CHOP, GADD34, and PUMA genes was also observed, but no increase in the expression of the CAS-4 mRNA and key UPR markers was observed .
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PMC11023271
In contrast to the report, we did not observe any effects of scratching on the level of RIPK4 mRNA in HaCaT cells, either in the absence of serum (Fig 1A) or the presence of serum (S1A Fig).
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PMC7602170
Miettinenet and colleagues reported DOG1 immunostaining as positive in 96% (Table 1) .
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PMC8592717
Furthermore, it demonstrated significant therapeutic effects in preclinical animal models with neurodegenerative diseases such as AD, PD, and D-galactose-induced accelerated aging, in vivo .
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PMC9966931
H NMR (600 MHz, CDCl3) δ 8.24 (d, J = 7.4 Hz, 2H), 7.72–7.59 (m, 1H), 7.58–7.47 (m, 2H), 7.33–7.19 (m, 1H), 7.08 (dd, J = 8.0, 1.8 Hz, 1H), 6.97 (d, J = 0.9 Hz, 1H), 3.08 (hept, J = 6.9 Hz, 1H), 2.36 (s, 3H), 1.23 (d, J = 6.9 Hz, 6H). (
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PMC11783017
Also, the levels of various blood biochemical markers in each group were quantified through blood biochemical analysis (Fig. S23).
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PMC10454535
Currently, BTZ is used clinically to treat MM as the first-generation proteasome inhibitor.
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PMC9429973
Cytogenetic analysis showed the Inv 16 (p13;q22) in 3 cases (18%) and t(8;21) (q22;q22) were observed in 11 cases (68%).
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PMC11551844
The H-NMR spectrum of JC-2 showed characteristics of a sterol moiety due to the presence of a multiplet at δH 3.5, which was attributed to the proton at C-3.
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PMC11787651
My time in Don’s lab was filled with what I feel was the most technical and even biological knowledge I have ever been exposed to in a short period of my career.
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PMC9429973
A complete remission was obtained in one patient, and a partial remission in two patients.
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