PMCID
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PMC11767793
Optimized DMEM was provided by AddexBio (San Diego, CA, USA).
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PMC10114490
diHLC-T, n = 6; HepG2, n = 3; *p < 0.05, **p < 0.01,***p < 0.005) Once determined the stability of diHLC-T with PD by HCS, we applied untargeted metabolomic analysis to establish their applicability for toxicometabolomic studies compared to HepG2, the most commonly used cell line in pharmaco-toxicological studies.
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PMC11790971
c Example multimodal CHO cell images obtained from an artificial pool containing all four cell lines in passage 2.
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PMC11392912
To measure Clo-miR-14, the total miRNA of Curcuma longa (200 ng) was mixed with 10 nM stem-loop primer (1µl), incubated at 60°C for 10 min for cDNA synthesis as per manufacturers guideline (QUIGEN, U.S.A.).
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PMC11739484
In this study, employing a comprehensive methodology encompassing both in silico analyses and molecular experiments, we identified significant dysregulation of MMPs in SKCM, with MMP7, MMP11, and MMP14 emerging as key players linked to tumor progression, survival, and diagnostic potential.
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PMC11568601
Afterwards, the cell apoptotic rate was calculated via a flow cytometry (BD Biosciences, CA, USA).
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PMC11342785
F, quantification of synMAP circuit induction for the panel of TPPP-varying designs from (E), using the method described in Figure 5D.
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PMC9429973
Methods: The clinical data, including the types of pathogenic microorganisms and their resistance profile to commonly used antibiotics, of patients developing bloodstream infection during hematopoietic stem cell transplantation from January 2020 to December 2021 were analyzed retrospectively.
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PMC9878278
To further validate the association between the antiviral response against ZIKV infection and BMP6 signaling, we first tested if BMP6 expression is under the control of IFN-β in SC and two other cell types (A549 and BMVEC).
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PMC11708541
3-aminopyridine-2(1H)-one, acylates efficiently in CH2Cl2 in pyridine to form the chloroacetamide in a good yield.
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PMC11257988
The difficulties in targeting KRAS are due to several factors: (1) the broad scope of KRAS’s activity, including its essential role in many normal cellular functions, meaning drugs that directly inhibit KRAS may have significant toxicity and strong side effects; (2) KRAS’s primary functional domain involves a pocket that binds to GDP or GTP.
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PMC7334607
Finally, this work could be improved by examining how collateral drug response in EWS changes during relaxed selection after many drug cycles of the treatment regimen have been applied (Card et al., 2019).
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PMC11661040
Fig. 5CGREF1 modulates osteosarcoma cell proliferation via the GSK3/β-catenin signaling pathway, and activation of the Wnt signaling pathway can counteract the impact of CGREF1 knockdown on osteosarcoma cells. (
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PMC11658074
The CK-MPA10 results revealed reduced melanin levels and improved elasticity in the fat graft group (Fig. 1f, g).
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PMC11787651
A shorter gradient consisting of 5 to 30% B in 5 min with a total run time of 11 min was also selected as being suitable for the analysis of histone PTMs in applications that may prioritize time savings over maximizing the depth of information obtained, such as screens of large-batch cohorts or analyses concerning large changes in the distribution of histone PTMs.
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PMC11087055
COS cells expressing either a control-GFP (control-GFP/COS) or Vg1 (Vg1/COS) construct were placed at the anterior marginal zone (AMZ) at pre-streak stage, approximately 180° from the posterior marginal zone where the authentic PS forms, and we analyzed the resulting cell flow during both authentic and ectopically induced PS extension (Figure 3B).
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PMC11643491
The PVA films were dissolved in an isopropanol–water mixture (3:7 v/v) under moderate heat.
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PMC9429973
Providing preparation and support for caregiver ambassadors was important.
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PMC9895440
For the culture of HEK293T cells, DMEM (Sigma) was supplemented with 5% heat-inactivated (56 °C 30 min) fetal bovine serum (FBS, PAN Biotech), 100 ng/ml penicillin/streptomycin (Sigma), 2 mM L-Glutamine (Sigma), 1 mM Sodium Pyruvate (Sigma) and 1% MEM non-essential amino acids (Sigma) and 10 mM HEPES (Sigma).
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PMC11756514
Green fluorescence protein (GFP) expressing S. aureus Reynolds (CP5), Reynolds (CP8), or Reynolds (CP-) were grown overnight at 37°C on Columbia agar with 2% (w/v) NaCl (CSA) or in suspension in Todd Hewitt broth (THB) at 37°C with shaking.
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PMC9031474
Based on 9-epipolygodial (49), Wittig derivative 54 was synthesized leading to reduced cytotoxicity compared to 49 (Scheme 2) .
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PMC11185260
Mice received total 6 injections of scL-SMARCB1 (30 μg/injection, twice weekly for 3 weeks, N = 7 for untreated group, N = 8 for scL-SMARCB1 group). (
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PMC10366908
LN229, mEGFR-overexpressed LN229 (LN229/mEGFR), and NMuMG were cultured in DMEM (Nacalai Tesque Inc.), supplemented as indicated above.
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PMC9599726
TFRC staining was graded as negative (−), weakly positive (+), moderately positive (++), and strongly positive (+++).
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PMC11723947
We also observed that the allogeneic T-cell response was characterized by the differentiation of a small proportion of IL-17A-producing cells (Fig. 2a, c).
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PMC4659567
Herein we report the synthesis and characterization of the complexes of the general composition [Ru(η -p-cym)(naza)Cl2] (1–8; p-cym = p-cymene) containing 7-azaindole or its derivatives (naza).
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PMC11657744
Data are reported as means ± SD of three independent experiments.
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PMC11543853
However, distinguishing enterovirus virions from activated particles, both of which contain genomes, requires high-resolution information, since the two structures differ only in the details of their capsids (Supplementary Table 4).
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PMC11742290
High LGR5 expression has been shown to be associated with increased tumor migration in cancers including gastric , CRC , breast , and HCC cancers.
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PMC2196094
Fragments of sizes from 165 to 220 bp from the 3′ terminal end of the cDNA clones encoding RMCP-1 (R-I), RMCP-2 (R-II), RMCP-3 (R-III), RMCP-4 (R-IV), RMCP-6 (R-VI), and RMCP-7 (R-VII) were excised and inserted into the plasmid vectors pGEM-blue or pGEM-2.
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PMC11185260
p<0.05. (
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PMC8753818
CHO-K1 and CHO-KO cells were maintained in RPMI medium supplemented with 10% fetal bovine serum as described previously .
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PMC11509309
In order to determine whether the amount of PrP was modified by the treatment with the molecules, cell lysates (250 μg) were digested by 20 μg/mL of Proteinase K (PK, Thermo Fisher Scientific, #EO0491).
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PMC11467964
Ultimately, we verified the eight BMRGs expressions in the novel signature, finding these signature genes had abnormal expression in STS, which indirectly confirmed the reliability of our analysis.
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PMC11089031
Then, enzyme and buffer were added to the RNA mixture and ddH2O was added to makeup total volume to 20 μl.
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PMC11218145
Twenty NOD-SCID mice pseudo-orthotopically inoculated with human MPM Ren cells were evaluated by BLI for tumour take rate and dimension, stratified into two groups of ten animals each and randomly assigned to treatment and control groups.
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PMC11655536
Interestingly, lysosome was among the top 9 pathways in the KEGG analysis of the up-regulated genes (Fig. 3A).
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PMC11711347
Molecular docking studies identified the active sites for the estradiol-estrogen receptor-α protein.
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PMC9429973
Published data are scarce on this subject.
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PMC10728535
Cisplatin serves as a highly effective chemotherapy drug for the treatment of a wide variety of cancers such as OC.
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PMC9429973
S. Suma, M. Fujisawa, Y. Abe, Y. Suehara, D. Kaji, T. Sugio, K. Kato, K. Akashi, K. Matsue, N. Nakamura, A. Suzuki, Y. Suzuki, S. Chiba, M. Sakata-Yanagimoto Department of Hematology, Graduate School of Comprehensive Human Sciences; Department of Hematology, Faculty of Medicine, University of Tsukuba; Department of Hematology, University of Tsukuba Hospital, Tsukuba; Department of Hematology, Toranomon Hospital, Tokyo, Japan; Department of Medicine, Division of Oncology, Stanford University, Stanford, United States of America; Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Science, Fukuoka; Division of Hematology/Oncology, Department of Internal Medicine, Kameda Medical Center, Kamogawa; Department of Pathology, Tokai University School of Medicine, Isehara; Department of Computational Biology and Medical Sciences, The University of Tokyo, Tokyo; Division of Advanced Hemato-Oncology, Transborder Medical Research Center, University of Tsukuba, Tsukuba, Japan Background: Angioimmunoblastic T-cell lymphoma (AITL) and other nodal lymphomas of T follicular helper (TFH) cell origin (TFH lymphoma) are associated with immune dysregulation.
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PMC11026382
We observed that R127A was more deleterious to catalytic function than Q33S, but that both mutations were more active than R166Q (which shows almost no measurable activity in vitro, Fig. 2b, Supplementary Table 3).Fig.
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PMC9910796
This distribution resembles that of pEP84R when expressed individually, although with a more compact and perinuclear distribution. (
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PMC9917080
However, sorafenib in the nanocomplex with selenium, on the contrary, contributed to the induction of apoptosis.
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PMC7433824
Therefore, we matched 9 and 32 unique genes in the U87 cell line with 100 µM P4 and 300 µM P4, respectively, with the differentially expressed proteins.
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PMC9429973
Patient matched bone marrow and blood samples were obtained for each donor at three different time points across the course of the treatment.
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PMC9429973
Haplo BMT was performed with PTCy.
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PMC10840195
SPSS statistics software (version 23; IBM, Armonk, NY, USA) was used for statistical analysis.
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PMC11449273
We show for the first time that plakoglobin differentially affected the oncogenic properties of p53-R273H and p53-R175H mutants expressed in the same cell line–H1299.
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PMC9844987
Indeed, decomposition of multi-modal peaks within the CAGE TSS signals of promoters (Supplementary file 5) demonstrated that higher entropy reflects an increased number of decomposed promoters, as indicated by their number of local maxima of CAGE signals (Figure 4B). (
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PMC10582049
In fact, Grout et al. have assumed that the ADH1B subpopulation of CAFs acts as a transient form between normal fibroblasts and FAPaSMA CAFs.
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PMC11737091
p < 0.05; **, p < 0.01; ***, p < 0.001 Knockdown of DBF4 expression blocked the cell cycle in ccRCC cells. (
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PMC10204952
A significant difference was defined by p < 0.05 compared to the vehicle control under different types of cell culture media series (*complete media, AD-CM and CAA-CM).
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PMC9429973
T. Brandstoetter, B. Maurer, J. Schmoellerl, S. Kollmann, J. Huuhtanen, R. Grausenburger, S. Mustjoki, J. Zuber, V. Sexl Dept.
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PMC9105697
Tumor detection occurs independently of the anatomic site, which is especially important for detection of extra-pulmonary metastases.
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PMC9587866
The study was designed to gather potential inhibitors for the EYA3 target using medicinal compounds.
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PMC10291556
This favors the new Pt–DCF complexes over the clinically used oxaliplatin in terms of their ability to induce ICD in cancer cells.
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PMC11704135
Furthermore, another study investigating the antifungal activity of pyrimidine derivatives has demonstrated their inhibitory effects against Aspergillus flavus, A. fumigatus, Candida albicans, and Penicillium marneffei strains.
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PMC5941560
Our WGS approach identified nearly 10x as many SNVs as were detected by COSMIC via microarray.
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PMC9429973
they had an undetectable viral load and persistently low CD4 count (< 200 /mm3).
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PMC11514418
APS is primarily sourced from Astragalus, which has the functions of tonifying Qi, consolidating the surface, strengthening the spleen, and benefiting the lungs and liver.
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PMC11680049
Large accumulations of epiblasts were observed in both the central and peripheral regions of the callus tissue (Figure 7 and Figure 8).
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PMC7136814
The protein expression of MYOD1 and Noggin genes in RD and A-204 cell lines were evaluated by flow cytometry to assess the distribution of these markers among the cell population.
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PMC10810426
The same experiment was performed in BMDCs.
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PMC11637820
The fast-halo assay was performed as previously described (23).
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PMC9966931
Eugenol-derived molecules were ten-fold less toxic than thymol or carvacrol hybrids, and benzoic derivatives were slightly more active.
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PMC9100622
After fixation with 4% formaldehyde solution, cell nuclei were stained with 0.2 µg/mL of DAPI and image acquisition was performed using the IN Cell 2200 automated wide-field microscopy system (GE Healthcare) at 10× magnification.
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PMC11188874
If KO efficiency was <80%, a second round of sgRNA delivery is performed.
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PMC8956657
A list of hydrogen-bonded interactions is presented in Supplementary Table 9.
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PMC11343332
Gene expression analysis was performed using quantitative real-time PCR to determine the levels of proapoptotic and vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR) genes mRNA expression.
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PMC7351993
The probability of each scenario is given by the product of the probabilities of all the five parameters.
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PMC3765139
The hybridization cocktail (130 μL) containing fragmented biotin-labeled target cRNA at a final concentration of 0.05 μg/μL was transferred into an Affymetrix Human Genome U133A 2.0 and incubated at 45°C on a rotator in a hybridization oven 640 (Affymetrix) for 16 h at 60 rpm.
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PMC10729469
Table contains p-values for slopes and intercepts of Treg functions compared by linear regression analysis, n = 3.
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PMC10818573
The RPA amplification products were subjected to agarose gel electrophoresis.
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PMC9429973
The median age of patients was 60 years (interquartile range: 49-75 years).
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PMC10317042
GO data are available in Supplementary Tables S3 and S4.
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PMC7081114
Thus, silencing the expression of KIF21B inhibited cell proliferation.
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PMC11413393
Figure (b) Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
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PMC8345486
A comparison of the IC50 values is shown in Figure 2A,B. In general, adenine nucleosides modified with metallacarboranes significantly decreased cell viability, regardless of cisplatin resistance.
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PMC11543853
The duration of their exposure to those endosome conditions also differs.
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PMC11591038
This evidence indicates the critical role of HIF-1 in AD patients with ApoE4.
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PMC11319300
In the pre-B cells only, interactions between 19 and 22 months mice (“old mice”) were included (Koohy et al., 2018).
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PMC7334607
In many cases, it is clear that ranking samples by their change in drug response also ranks them based on how long they have been exposed to the treatment regimen.
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PMC11798926
Scale bar: 10 μm.
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PMC11422150
In our experiments, a significant improvement was observed in three categories.
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PMC11201989
Hh signaling is associated with increased levels of pathway mediators along the primary cilium including Gli2 and Gli3 .
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PMC11740399
This indicated that 3025@ML exerts an antitumor effect by inducing apoptosis.
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PMC9429973
These may have been otherwise undetected, but can subsequently be confirmed by FISH, and importantly allow the generation of molecular measurable residual disease (MRD) assays to assess treatment response.
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PMC10820104
This assignment was suggested from the molecular composition of 1 (C30H41N3O4), which, compared to rubrumline I (2) (C25H32N3O4), bears an additional isoprenyl group (-C5H9) constituting the last remaining degree of unsaturation.
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PMC10840195
The migration ability of cells was determined by a cell scratch experiment (B).
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PMC11732523
Zhisheng Chen: Data curation.
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PMC10938336
A comprehensive analysis was carried out with the retrieval of 25,001 disease-related target predictions from the GenCard data set.
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PMC11774251
Results are manifested as mean values accompanied by their standard deviations (±SD).
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PMC11237030
Though, to our knowledge, no human AQP5 stable cell line is currently available.
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PMC6268318
The effectiveness of the plant extracts depended on the type of drug studied and the bioassay models.
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PMC9429973
TK levels (p<0.001) and TPA (p<0.011) were also significantly higher in group 2 (7.7 and 49.8 IU/L) compared with patients in group 1 (4.2 and 34.3 IU/L).
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PMC11521786
All animal experiments were approved by the Center of Molecular Immunology’s Institutional Animal Care and Use Committee (CIM, Havana, Cuba).
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PMC10094992
Once in the tumour tissue, the microenvironment cues are responsible for the polarization of tumour associated neutrophil (TAN) towards either anti-tumoural or pro-tumoural phenotypes .
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PMC11752740
Our results indicate that the combination of pistachio hull extract and CFZ at the effective dose (ED50) produced notable synergy, with combination index (CI) values of 0.102 and 0.0221 for the SK-BR3 cell line at 24- and 48-hours, respectively.
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PMC11576293
4H, Ar-H), 7.69 (s, 1H, pyrimidine–C5-H), 7.49 (m., 4H, Ar-H), 7.31 (m., 3H, Ar-H), 7.03 (d, 2H, Ar-H, J = 9 Hz), 4.6 (s, 2H, SCH2-), 3.91 (s, 3H, OCH3); C NMR (75 MHz, CDCl3) δ (ppm) = 171.9, 164.4, 163.3, 162.2, 138, 137, 135.4, 129.1, 128.9, 128.5, 127.1, 114.3, 107.1, 55.5, 35.5; HRMS (ESI+) calcd.
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