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PMC3122064
Finally, the importance of the Fas death receptor pathway in Nutlin-3 induced apoptosis has been studied.
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PMC11786704
Percentage of closed diabetic wound area at different time points. (
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PMC11665555
The downregulated genes included annexin A6 (ANXA6, involved in autophagy), troponin T3 (TNNT3, involved in skeletal muscle development), and peptidyl arginine deiminase, type II (PADI2, involved in protein citrullination) (Figure 4D) (35–37).
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PMC11705862
Further, Navenibodies (antibodies conjugated to proprietary oligonucleotide arms), which bind to their respective primary antibodies were added for 1 h, 37 °C.
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PMC11634027
N′,O′) Rose plots showing quantification of hair polarity from the wing region boxed in N, from ds/ds (N′) and ds/ds (O′), with number of hairs measured indicated by n. ns, not significant.
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PMC10656496
As the genomic characterization was highly consistent with prior publications, we then shifted to analysis of the phenotypic features of osteosarcoma cells in different environments, including manipulation of pH and nutrients and exposure to chemotherapeutic agents.
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PMC11655498
Similar to ZEB1, ZEB2/SIP1 can bind to the same sites on Zp and inhibit BZLF1 expression to maintain EBV latency (Ellis et al., 2010).
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PMC9429973
In order to assess the consistency of PRO reporting, study registries were compared with publications and study protocol, where available.
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PMC11621493
To get insights into the positional preferences of PLA2G4D, we compared DAG formation using rac-18:1 MAG or 2-18:1 MAG as substrate (Fig. 3H–J).
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PMC11519077
In the AD-MSC group, the expression of IL-12 was decreased compared to the IL-1β-induced Caco-2 group, while it was significantly increased compared to the control group.
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PMC11295144
The cells were co-cultured with medium spiny neurons and synaptic activity in HEK293 cells was examined using whole-cell recordings.
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PMC11301242
The processed WGS or CIRCLE-seq FASTQ files were aligned to the GRCh38 using BWA-MEM.
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PMC11775197
In the HCT-116 CRC cell line and mouse colon tumor epithelial cells, GW501516 activated PPARβ/δ, which further induced an increase in the expression of COX-2 and its derivative PGE2 and led to an elevated expression of pro-inflammatory mediators in the colonic mucosa, including CXCL1, CCL2, CCL3, CCL4, and IL-1β.
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PMC11627133
Among the fluorescent light‐up aptamers (FLAPs) originally developed for RNA imaging, RhoBAST stands out due to its remarkable brightness, photostability, fluorogenicity, and rapid exchange kinetics, enabling super‐resolved imaging with high localization precision.
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PMC11551844
The data were normalised to the control, and the IC50 values were calculated using GraphPad Prism software version 8.4.3.
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PMC11675244
As mentioned earlier, due to the intricate interactions between Wnt ligands and their receptors, using Wnt ligands with known inhibitory effects has been met with hesitation.
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PMC9429973
Experience outside clinical trials has been limited due to the rarity of the condition.
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PMC9429973
Meldola, Italy Background: Our previous work showed that Arginase (ARG-1) and Indoleamine Deoxigenase (IDO-1) are increased in tumour microenvironment of classic Hodgkin Lymphoma (HL), but the consequences of arginine (arg) and tryptophan (trp) deprivation on neoplastic cells fitness are largely unknown.
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PMC9429973
Summary/Conclusion: This functionally high-risk pt population with early relapse after initial therapy experienced deep and durable responses with manageable safety following a single cilta-cel infusion.
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PMC11301242
MF: molecular function; CC: cellular component; BP: biological process. (
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PMC9429973
Primary study outcome was to identify a role for FXI levels in the thrombotic risk of aPL patients.
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PMC11799620
Many scientific reports associated health damage with cadmium-induced oxidative stress (Bojarski et al., 2022).
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PMC11740399
Moreover, the drug’s presence in plasma and organs indicates effective systemic distribution, i.e., absorption, metabolism, and circulation, supporting its observed anti-tumor effect.
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PMC8345486
These findings indicate that metallacarborane modification of adenosine may sensitize ovarian cancer cells to cisplatin in combination treatment.
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PMC11354225
Chelation of transition metal ions can interfere with the delivery of metal ion cofactors to receptor kinases and regulate enzymes .
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PMC11626627
Validation of the effects of ERβ and TWIST1 was performed using Western blotting (A) and qRT-PCR (B) in 786-O cells transfected with pLKO.1, sh-ERβ#2, oe-TWIST1, and sh-ERβ#2+oe-TWIST1.
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PMC11092382
Benth.
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PMC9874064
103.8 ​nm (Fig. S6), while that of NP-Pt-USP1i was about 124.2 ​nm (Fig. 2B).
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PMC9429973
Both patients received a single dose of RM-001 cells, ceased pRBC transfusions within 1 month after RM-001 infusion and remained transfusion-free through the reported period.
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PMC11787355
To stain the slides, they were first air-dried and pre-treated with RNAscope Hydrogen Peroxide.
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PMC9712534
We therefore investigated whether the H58Y substitution may affect the nuclear dynamics of TOP2B.
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PMC11045125
The P3HR1 Burkitt lymphoma G668 (Cl 16) cell line was a gift from George Miller at Yale University via Bill Sugden at UW-Madison .
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PMC9429973
Baseline clinical characteristics, TTFT, first line therapies, and OS were analyzed for all patients.
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PMC10944868
The spatial congruence in their complex formations suggests a potential similarity in their binding mechanisms and affinities for specific regions on the tubulin structure.
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PMC11763126
C Western blot analysis showing YTHDF2 levels in each group.
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PMC6160470
The main function of ionophores is their ability to transport ions across lipid membranes.
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PMC11711871
Physiological parameters such as body weight, food and water consumption, the amount of urine and feces, and the plasma and urinary electrolytes Na and K were measured at weeks 1 (days 1–5), 3 (days 16–21), and 5 (days 32–37).
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PMC9856122
This phytoconstituent and its natural analogues showed satisfactory results when evaluated collectively by in silico and in vitro methods.
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PMC11548041
This method involves screening of a large oligonucleotide library pool, with PCR amplification of the positively selected candidates, followed by iterative rounds of screening .
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PMC11267036
On the other hand, since this study revealed that the activation of the STAT1/RIG-I pathway by DHM is beneficial for the treatment of MM, future research can focus on this pathway to develop targeted agents for enhanced therapeutic effects.
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PMC11381192
Hypoxic stress is regarded as a stress situation in which a specific tissue is supplied with less oxygen than required.
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PMC11680578
Ovarian cancer, a diverse disease with a 5-year relative survival rate and bleakest prognosis, is a prevalent form of gynecological cancer.
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PMC11761919
Detection of Fiber2-specific sera IgG by indirect enzyme-linked immuno sorbent assay (ELISA). (
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PMC6461034
A major challenge is to relate such profiles to specific hyperactive kinases fueling growth/progression of individual tumors.
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PMC10577955
The acetylation of 3-OH was further confirmed from the correlations in HSQC and HMBC between the C = O (at δC 170.9 ppm) and H-3 at δ H 4.46 ppm.
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PMC11521786
The antitumor potential of this formulation and the induced PAbs was demonstrated in vivo and in vitro in an ErbB3-overexpressing 3LL-D122-derived tumor model.
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PMC11502443
Relative efficacy plot of Δlog (τ/KA) values for the E354Q GIP receptor, where GIP(1-42) is the reference peptide. (
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PMC11347429
Information on gene symbols and names from Gene Cards (GeneCards), information on gene functions from (Alliance_of_Genome_Resources) and subsequently edited manually.
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PMC11790971
In addition, several traditional classification evaluation metrics were calculated, including balanced accuracy, F1-score, precision, recall, Jaccard score, and Area-Under-the-ROC-Curve (AUC).
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PMC11412721
Figure 2—figure supplement 1.Analysis of tumors from Trp53 Eμ-myc and Trp53 Eμ-Myc mice.(A) Histological analyses of Eμ-Myc-induced tumor lymph nodes.
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PMC11806106
Our MOR FRET sensor revealed the on-rate for different opioid agonists.
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PMC10734950
The confirmation of Caspase 3/7 activation indicated that F8 initiated the intrinsic apoptotic pathway as its mechanism of action.
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PMC11658074
g PKH26 and immunofluorescence staining of CD151 in cells after incubation with PKH26-labelled CD151( +) AT-EVs and CD151(-) AT-EVs for 30 min.
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PMC10490530
These cells were either left untreated or treated with TNFα or doxorubicin or both for specified concentrations and time, as indicated in the results.
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PMC10530622
A disrupted microbiota (a dysbiotic microbiota, with either reduced commensal bacteria, increase of pathogens, or an overall reduction in diversity and richness ) is associated with poor health outcomes.
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PMC8409415
Several chemotherapeutic and target therapy agents have been evaluated in clinical trials for recurrent OvCA, including cytotoxic agents, target therapy, poly (adenosine diphosphate‐ribose) polymerase inhibitors, and immune checkpoint inhibitors; however, these agents exhibit only modest clinical responses., , , , ,
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PMC10197932
For in vitro activation markers analysis, we used anti-CD3/CD28 DynaBeads + 40 U/ml recombinant Il-2, and recombinant Il-7 at 15 U/ml as described by the manufacturer protocol 25.
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PMC11656380
Tat-Ram13 triggers fundamental cell death processes, including mitochondrial membrane depolarization and plasma membrane rupture.
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PMC11001582
Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance.
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PMC10092581
After washing with buffer and post‐fixation with 1 % OsO4 in 0.2 M cacodylate buffer, specimens were dehydrated and embedded in epoxy resin (Epon Araldite).
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PMC9429973
In the Netherlands, population-based referral and eligibility assessment for CAR T-cell therapy are centralized in a national tumorboard.
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PMC9429973
In independent external validation, the SVM model also exhibited an optimal sensitivity of 0.600 and an F1 score of 0.667.
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PMC11750165
To confirm whether PEX1 mutation affects the specification of KA” neurons, whole-mount in situ hybridization was performed using the GAD1 riboprobe (Fig. 5).
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PMC11413393
Our data suggests that type I PRMT inhibition leads to a pronounced downregulation of the cell cycle, compromised DNA damage repair (DDR) pathways and an accumulation of double-strand breaks (DSBs).
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PMC11612508
Eco-friendly substances like PEG and chitosan have functionalized GO with safer surface functional groups.
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PMC8414691
P < 0.05; **P < 0.005; and ***P < 0.0005 compared with the mock group by Fisher’s exact test Cytokinesis regulators mislocalization in SRCAP depleted HeLa cells The results are expressed as mean ± SD values from three independent replicate experiments: *P < 0.05; **P < 0.005; and ***P < 0.0005 compared with the mock group by Fisher’s exact test The aforementioned results are also suggestive of possible interactions between SRCAP and cytokinesis regulators during telophase.
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PMC5535901
Cells at a density of 0.3 × 10/mL were plated in T-75 flasks overnight.
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PMC9429973
ABP 959 and the RP are humanized recombinant IgG2/4κ mAbs which bind with specificity to the human complement C5, blocking its cleavage to C5a and C5b and preventing the generation of the terminal complement complex (TCC), thereby blocking cell lysis.
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PMC11615828
FRET imaging utilized CFP, YFP, and CY‐FRET filters, with the FRET/CFP ratio calculated by the FRET module in the NIS‐Elements AR software.
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PMC11756514
This finding was confirmed when a range of different MBL concentrations in the binding reaction was tested ( Supplementary Figure S6A ).
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PMC11711063
Conversely, prevention of cancer requires the suppression of enabling factors, such as proliferation, a favorable microenvironment or the creation of immune‐privileged sites.
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PMC8658661
A change in the level of DLK1 expression was also observed in tumor cells, and the gene was more actively expressed in HB cells than in HCC .
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PMC9429973
The estimated median PFS was 5.9 years [95% CI: 5.3-not reached (NR)].
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PMC9429973
Results: 332 genes were differentially expressed in CD3+ lymphocytes one month after lenalidomide treatment in our cohort of patients; of note, none of them were observed after one month in non-treated patients.
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PMC6889484
p90CF402.1 is an intersubtype recombinant AE HIV-1 infectious molecular clone, obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH (from Drs.
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PMC10674574
Towards confluence, they begin to polarize, forming a monolayer with strong, tight junctions, apical brush borders, and microvilli.
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PMC3019555
Arrows show colocalization of Ago2 and GE-1. (
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PMC11753435
β-Actin was used as the loading control.
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PMC11402217
All experiments were conducted in triplicate, with data presented as mean ± SD from representative experiments (n ≥ 3).
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PMC9429973
Finally, 729 proceeded to lymphodepletion and CAR-T infusion.
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PMC11394730
Inhibiting glucose transporters in neoplastic cells is a promising cancer treatment method that seeks to create an energy-deprivation condition that can enhance the effects of other anticancer medicines .
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PMC11742670
A lower MSE indicates superior forecasting model performance.6[12pt] $$\:MSE=_-_})}^}$$ Where is the ith observed value, is the corresponding forecasted value, and is the number of observations.
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PMC11555465
Typically, 1×10 bladder cancer 5637 cells and T. gondii tachyzoite culture supernatants + bladder cancer 5637 cells (10:1) in serum-free media were separately seeded into the upper chambers with (invasion) or without (migration) Matrigel coating (diluted in RPMI-1640; Corning, Inc.; 4°C for 3 h) and the bottom chambers were filled with 600 µl supplemented RPMI-1640 medium.
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PMC7887261
Non-targeting pool siRNA (D-001810-10), SMARTpool siRNAs each containing four targeting sequences of MME, MLNR, POMC, ATM, CHEK2, and MPZL2, sets of 4 siRNAs targeting MME, MLNR, and POMC were purchased from Dharmacon.
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PMC11763126
We utilized IP coupled with mass spectrometry analysis to delve deeper into the mechanism through which CBDP1 modulates the ubiquitination and degradation of YTHDF2.
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PMC6379402
For each clustering, the bars show the percentage of the reactome pathway (RP) annotated genes having at least one annotation that is enriched in the clusters.
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PMC5363531
Cells were then transfected with JetPrimer (PolyPlus, Strasbourg, France) using 2 μg of plasmid 1:10:1 combinations: pGL4-TS-Prom-1191+176/TK-renilla/pdDNA 3 or pGL4-TS-Prom-1191+176/TK-renilla/pcDNA-SRC-KD-K295M per well according to manufacturer's instructions.
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PMC8751435
Effect of Cordyceps militaris extract (CME) on cell apoptosis. (
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PMC11216402
Each gkm-SVM model generates a score function that can be specified as weights for each gapped k-mer (Ghandi et al. 2014b), and the score for each sequence is the sum of the weights for all gapped k-mers in a sequence.
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PMC11360263
Polyploid giant cancer cells (PGCCs) may possess either a single enlarged nucleus or multiple nuclei, denoted as mononucleated PGCCs or multinucleated PGCCs, respectively .
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PMC11544122
No. UFC903024).
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PMC9429973
This cohort was divided into 2 groups: 1st group (49 patients) without signs of progression of CLL, and the 2nd group (58 patients) with clinical signs of disease progression after 3 years of observation.
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PMC10813895
Such plasticity could explain the diversity in ELF5 expression among ER+ cancers, potentially depending on whether the cancer cells originate from ER+/ELF5− or ER−/ELF5+ luminal epithelial cells progenitors.
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PMC11411131
The amount of DNA used for transfection was either 100 ng or 10 ng (with 90 ng of mock plasmid DNA) of the indicated cDNA. (
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PMC11754291
Before each light treatment, the light module was calibrated using a UNO power meter (Gentec, Quebec, Canada) connected to a silicon-type photodiode (PH100-Si-HA-OD1-D0, Gentec, Quebec, Canada) and then positioned 12 cm from the cells.
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PMC11306331
Several optogenetic tools rely on a similar molecular logic, either through constitutive fusion of an IDR to a light-switchable oligomerization domain (e.g., PixELLs) or through light-dependent association between an IDR and a constitutive oligomerization domain (e.g., Corelets and CasDrop).
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PMC5386478
Increased expression of TG2 in cancer cells has been linked to drug resistance, metastasis and poor patient survival.
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PMC9429973
Samples are from CLL patients (N=47) with more than 98% blood tumor cell infiltration and controls consisted in healthy volunteers (HV) (N=9).
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PMC5035325
First, EGFR and NeuGcGM3 expression was analyzed by immunohistochemistry and second, the co-expression of both molecules was determined by immunofluorescence assay.
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PMC11670407
The integrity, quantity, and purity of the obtained RNA samples were determined by a microplate reader (Thermo Fisher Scientific, Vantaa, Finland).
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