PMCID
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PMC11472569
For instance, tumor-derived exosomes can reprogram cancer-associated fibroblasts (CAFs) to create an acidic TME, enhancing resistance to platinum-based drugs in non-small cell lung cancer (NSCLC).
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PMC7057940
However, this disease can develop in any other tissue or organ (extra-osseous ES) (2,3).
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PMC10882807
Additionally, EIF4A3 binds to circ_0004296, inhibiting metastasis of prostate cancer .
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PMC11762280
D Representative images of immunohistochemical staining of N-GSDMD, cleaved PARP, and p-MLKL in human skin samples from sun-exposed and non-exposed areas.
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PMC11594074
Based on this PAINS (reactivity), the subsets were categorized into the following groups: (A) anodyne (no acceptance of PAINS compounds); (B) clean (allows PAINS); (C) mild (containing PAINS patterns and weakly reactive, typically as a nucleophile or electrophile); (D) reactive; and (E) unstable or irrelevant for screening.
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PMC9429973
Thus, GCs combined with glycolysis inhibitors promise to be a novel therapeutic strategy for aGvHD patients.
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PMC7917457
In addition, we observed noticeably higher numbers of OVA-specific CD8 T cells in pooled tumor-draining lymph node samples from PeptiCRAd VALO-mD901-OVA-treated mice compared to mock- or PeptiCRAd Ad5/3-D24-OVA-treated mice (Figure 4B).Figure 4Virus-encoded OX40L and CD40L improve anti-tumor efficacy and induce robust infiltration of tumor-specific CD8 T cells into the tumor in a syngeneic mouse model of B16.OVA melanoma(A) 1 × 10 VP of PeptiCRAd Ad5/3-D24-OVA or PeptiCRAd VALO-mD901-OVA was given intratumorally 6, 8, and 20 days post-tumor implantation.
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PMC9429973
Sick mice were sacrificed, leukemia cells were isolated from five different organs (bone marrow, spleen, lymph nodes, spinal cord and brain) and characterized by immunophenotyping, sanger sequencing, bulk RNA sequencing (bulk RNAseq) of GFP+ sorted cells, single cell RNA sequencing and mass cytometry (CYTOF).
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PMC9454852
BOLD-100 has previously been shown to induce apoptotic cell death.
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PMC7570809
The branch points were counted to better evaluate the percentage of inhibition.
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PMC11464982
■■■■■.
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PMC9429973
C. Saha, V. Stables, E. Dimitriadou, J. Schofield, A. Green, D. Radia Hematology, Guy’s and St Thomas’s Hospitals NHS Foundation Trust, London; Hematology; Histopathology, Maidstone Hospital, Maidstone; Cellular Pathology, Guy’s and St Thomas’s Hospitals NHS Foundation Trust, London, United Kingdom Background: Systemic mastocytosis (SM) with an associated hematologic neoplasm (AHN) comprise approximately 70% of patients with Advanced SM (AdvSM).
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PMC11578343
Heatmap of the measured elastic G′ modulus for each single cell in each zone, grouped by Lat‐A‐treatment and untreated control conditions.
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PMC9429973
Cells were non-programmed frozen and stored at -80ºC in a solution with final concentrations of 5% DMSO, 3.6% hydroxyethyl starch (HES 450 000 mw) and 3% of human serum albumin.
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PMC11547917
Furthermore, Trolox showed a higher antioxidant effect on Caco-2 cells compared to the extract (Figure 3).
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PMC8623816
Subsequently, S1 nuclease (active against ssDNA or ssRNA) and DNase I (active against ssDNA and dsDNA) were used to confirm the properties of the extracted viral nucleic acids.
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PMC11569208
Each siRNA was assigned calculated values expressed as (1) fold-change in viral titer over infected cells transfected with a universal negative control and (2) oncolysis enhancing factor defined as the logarithm of the viability of transfected cells divided by the viability of transfected infected cells.
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PMC11564322
The cell co-treatment with the c-SRC inhibitor I and the VGVAPG or VVGPGA peptides increased the mTOR protein expression compared to the treatment with the VGVAPG or VVGPGA peptides alone (increase by 7844.41 and 1967.48%, respectively) (Fig. 7D).
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PMC11742431
Therefore, 5637 cells were selected to verify the degradation of the Survivin protein.
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PMC11371627
Muscles in other parts of the body were not affected. (
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PMC11753435
In the presence of the proteasome inhibitor MG-132, no β-catenin degradation was observed, indicating that the reduction in β-catenin observed with the uAb constructs is proteasome mediated (Fig. 4C).
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PMC9436459
Guangsangons M and N (114 and 115) are a pair of the 2-epimers, which were isolated from the stem barks of M. macroura by Dai et al. in 2004 .
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PMC4443654
Thus, the same protein was implicated at the level of broad genomic binding patterns (over 1-Mb intervals) and at the level of locally enriched peaks at boundary regions (spanning 100 to 500 kb).
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PMC11653533
Again, three flavone glycosides, quercetin 3-Orutinoside, kaempferol 3-O-rutinoside and kaempferol 3-O-robinobioside obtained from F. benjamina showed antivirus activity against Herpes Simplex Virus 1 (HSV-1) and Herpes Simplex Virus 2 (HSV-2) with selectivity index (SI) of 266, 100 and 666 respectively for HSV-1 virus (Yarmolinsky et al., 2012).
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PMC10362265
Furthermore, this epitope holds the C74 involved in the novel intermolecular disulfide bond in the recently described human tetramer .
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PMC11300085
Herein, we found that chemerin has a paradoxical effect on proliferation of OVCAR-3 and SK-OV-3 cells.
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PMC11488203
At least, the profound influences and critical regulations of many biological processes by miRs include growth, differentiation, apoptosis, motility and malignant transformation.
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PMC9429973
I. Serin, B. ORHAN, D. SONMEZ, T. A. CINLI, H. GOZE, H. SERIN, O. YOKUS Department of Hematology; Department of Medical Biochemistry, University of Health Sciences, Istanbul Training and Research Hospital, Istanbul, Turkey Background: Since it is not possible to access all methods simultaneously in determination of prognosis, it is important to use different methods together in acute myeloid leukemia (AML).
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PMC8609870
Besides, all the data were processed by quantile normalization.
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PMC9275501
The premature leakage of drugs from the nanoparticles at physiological pH was efficiently restrained because of the enhanced structure integrity, whereas at acidic and hypoxia microenvironment the release of both drugs was promoted due to the rapid dissolution of the CaP shell and the break of the disulfide crosslinked network.
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PMC11489351
We found that Notch1 expression in RPE cells was increased ∼2-fold at high pHi compared to low pHi (Fig. S5, B and C).
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PMC11806106
The agonist-induced activation was normalized to the maximal activation and plotted as concentration-response curve (B) (mean ± SEM, n = 3 of independent measurements performed in triplets).
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PMC11725127
The IC50 value of GA for inhibiting USP2 was determined to be 1.8 μmol/L. Data are mean ± SD (n = 3), P-values were analyzed by two-way ANOVA (F).
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PMC11401358
AFM characterization of SF, revealing a nanofibre-like structure (Scale bar = 400 nm). (
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PMC10728200
Colocalization of STUB1 (green) and GPX4 (red) in cells appears yellow in the merged images.
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PMC11055510
In view of this, the study aims to identify effective circular RNAs (circRNAs), which are biomarkers for OC, and explore their mechanism of action in OC development.
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PMC11740399
Tumor weight and Tumor volume. *
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PMC10174970
A total of 479 of potentially related datasets series was found in GEO, of which 86, 48, 226, and 12 datasets were excluded for treated cell line, duplicate data-series, unavailable detail information, and too many missed data, respectively.
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PMC3888431
Normalized libraries for both Roche/454 and Illumina sequencing were synthesized from poly(A) RNA using random primers to equally cover the transcripts.
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PMC9429973
Assessment of binding and activation of NK cells by AFM28 to test suitability for combination development with allogeneic NK cell therapy.
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PMC8010066
No stars: the non-statistical correlations The expressions of lasI (a), lasR (b), algD (c), KPC (d), and mexR (e), in P. aeruginosa.
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PMC11723947
It also correlated with an impaired mobilization of the regulatory circuits, which inhibit T cell-programming (Fig. 3c) and of gene sets involved in exhaustion (Fig. 3d).Fig.
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PMC10955424
Malignant ascites (n = 9, As004–013), cirrhosis ascites (n = 1, Cr004).
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PMC11757131
Previous studies in mice have found that the concentration of 3-HIB in plasma has become an important marker of the extent of mitochondrial fatty acid β-oxidation in mouse livers (32).
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PMC11392912
The macroscopic arthritic score was measured on the 28th day and represented by a graph based on naked eye observation and swelling (based on measured paw volume by plethysmometer) in the Clo-miR-14 treated group compared with the CIA and NC groups. (
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PMC9429973
LC advocates for increasing awareness of lymphoma among the general populace and the medical community to accelerate diagnosis.
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PMC11228511
After 48 h of Dox induction, SMCs were stained with an antibody that detects human lamin A and progerin (but not mouse lamin A/C).
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PMC11453018
The data represent mean ± SEM; *p<0.05, ****p<0.0001 by one-way ANOVA (n=3) P300/CBP Inhibition triggers senescence in ES cancer cell lines.
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PMC11765879
Briefly, a 2% stock solution of Noble agar (Sigma-Aldrich) was previously prepared in autoclaved deionized distilled water.
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PMC11453018
FDA-approved drugs such as vincristine, doxorubicin, and cyclophosphamide show some effectiveness but lack specificity for the EWS::FLI1-driven malignancy [39–41].
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PMC11763126
D PCR analysis of seven genes with significantly altered expression levels in ccRCC cells after CBDP1 treatment.
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PMC11549062
Western blotting images of Coimmunoprecipitation to test the interaction among PTP1B, Rab5, and PDGFR‐β. (
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PMC11224206
It may be of advantage that the egg is the largest cell in the female mammal and provides a much larger experimental target than the average somatic cell.
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PMC8557138
Tumor spheroid models to understand underlying mechanisms of angiogenesis 2D cell culture techniques do not faithfully replicate the in vivo mechanical and biochemical cellular and molecular interactions which lead to alterations in cell morphology, rate and plane of cell division, and also their physiological function.
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PMC11013014
Following 72 h of incubation, 20 μL of Cell Titer 96 AQueous One Solution Reagent (Promega Corporation, Madison, WI, USA) was added and incubated for 2 h before recording absorbance at 490 nm using a spectrophotometric plate reader (Promega Corporation, Madison, WI, USA).
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PMC11754784
As shown in Fig. 5b, if the expression level of CD47 is higher than PTK7, the Nb-S strand difussed into the surrounding solution is limited to an extreamly low concenration by the “diffusion trap”.
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PMC9635307
CEACAM1 has been reported to be expressed on activated, but not resting, T cells .
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PMC11564405
This sophisticated database, a testament to meticulous computational methodologies, provides a multifaceted lens through which researchers and clinicians can navigate the intricate terrain of immunological landscapes across a diverse spectrum of human cancers .
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PMC11720107
These garlic cultivars increased SOD1 protein expression.
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PMC11792766
Increasing ethanol concentrations exerted a cytotoxic effect on HepG2 and Caco‐2 cell lines, as shown in Figure 2.
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PMC11489351
After incubation in trypsin, 250 μl of prewarmed full culture media was added to each well to inactivate the trypsin.
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PMC9429973
Median age was 60 (18-90) years, 236 (61%) were male.
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PMC4270159
0.001 µM–10 µM sunitinib + 50 ng/ml FGF + 0.5 µM PD173074.
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PMC8633974
Whereas HIV-1NL4-3-ΔtatD proliferation approximated that of control HIV-1, HIV-1NL4-3-ΔtatE and HIV-1NL4-3-ΔtatDE remained at or around baseline for four weeks.
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PMC11321678
Different colors of background indicate subgroup specificity.
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PMC11574029
P < 0.05, **P < 0.01, ***P < 0.001.
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PMC11285179
This hypoxic microenvironment triggers alternative pro-angiogenesis molecular pathways in tumor or stromal cells within the tumor microenvironment, including FGF-2, HGF, DLL4/Notch, CCL28, and others .
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PMC11438317
PSTPIP1 Ab was generated against the whole protein produced in bacteria .
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PMC11534377
This observation points to a synergistic effect of the combined treatments in further reducing the invasive potential of A549 cells.
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PMC11738017
Lastly, noting the importance of adequate TCE dosing is the reduced TCE cytotoxicity in cells with low mBCMA at lower TCE concentrations.
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PMC11342785
Related to (Fig. S5) N here refers to the number of cells on which the FRAP was performed.
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PMC9429973
Responses were durable, with median durations of 239 days (range 48–587; median follow-up [mFUP] 247 days [range 21–690]) and 112 days (range 63–414; mFUP 121 days [range 22–464]) for 7/14- and 14/28-day schedules, respectively.
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PMC9429973
The cellular proliferation was reduced by ferric ammonium citrate (FAC).
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PMC11240571
This mixed transfection complex reagent was added to cells and incubated for 2 days.
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PMC11451940
X-ray quality crystals were obtained from a THF solution layered with pentane and settled aside at 30 °C.
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PMC11680578
Additionally, PPAR-γ is highly present in the cells that line tumor blood vessels and can hinder tumor growth by stopping angiogenesis.
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PMC11777207
Representative flow plots showing in the dLN 24 hours after polyI:C injection and FITC paint application.
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PMC9429973
Most patients with HHV-8 MCD were HIV-infected (73%); median CD4 lymphocyte count 0.229x10/L (range: 0.016, 1.2) and 46% of them had concomitant or prior Kaposi’s sarcoma.
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PMC7504302
Unlike other cancers that are generally rich in oncogenic gain of function alterations, genomic losses in MPM carcinogenesis are more frequent.
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PMC10820104
Based on NMR data, optical rotations and biogenetic considerations, the same absolute configurations, as elucidated for compound 2 via X-ray diffraction analysis, can be assumed for the other alkaloids as well.
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PMC11751675
These findings may contribute to refining the design of more effective HCV inhibitors.
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PMC11513571
The concentration of TSLP increased slightly at 72 h (32.4 pg/mL) and began to decrease at 96 h (19.2 pg/mL) (Figure 3A).
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PMC11352311
In addition, natural products such as genistein, kahweol acetate, and sinularin have demonstrated antitumor effects in RCC treatment through various mechanisms, including promoting apoptosis, inhibiting angiogenesis, and increasing chemosensitivity .
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PMC11791264
Vimentin (5741) antibody was purchased from Cell Signaling Technology.
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PMC11547917
To the best of our knowledge, no previous data were available for the A. clematitis ABTS assay.
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PMC11756514
However, we found that the presence of CPs convincingly shields S. aureus from recognition by all three CTLs, langerin, SP-D, and MBL.
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PMC9429973
The case selection process is summarized in Figure 1.
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PMC11387401
Next, we aimed to validate this HIV-1 protein–host RNA dataset by performing three complementary nRIPseq workflows on a focused set of HIV-1 proteins (Fig. 1B).
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PMC11367146
The clinical characteristics of OA include the breakdown of joint cartilage, thickening of subchondral bone, osteophytes development, inflammation of the synovial lining, internal joint inflammation, and changes in the structure of the joint capsule, ligaments, and surrounding muscles [, , ].
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PMC9712534
TOP2B modulates the expression of a specific subset of neuronal genes.
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PMC7294030
After 24 h, cells were harvested, washed, and fixed overnight in 70% ethanol at 4C, and incubated at room temperature for 30 min in the dark with RNase A (final concentration 2 μg/mL) and propidium iodide (PI) (final concentration 2.4 μg/mL).
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PMC11409031
Following this, a clustering tree was constructed using the 'PlotDendrogram' function, grouping genes into distinct modules.
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PMC8633974
The single mosaic gRNA CRISPR TatE and TatD constructs demonstrated the most significant suppression of HIV-1 replication (Figure 2a).
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PMC11394112
To confirm evidence that IL6 signaling is a critical driver of a subset of DLBCL and an indicator of engraftment and growth in NSG-IL6 mice , we treated U2932 and VAL cells with human IL6 and determined STAT3 activation.
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PMC11471176
Subsequently, Standards No. 2 to No. 5 were prepared by sequentially diluting the preceding standard with the diluent.
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PMC11474209
One promising development in water quality monitoring is the emergence of optical biosensors that detect microcystins.
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PMC9429973
AIHA diagnosis predated pregnancy in 15 women and had required at least 1 therapy line in all of them, and ≥2 lines in 12 (rituximab, N=7; cytotoxic immunosuppressants, N=6; splenectomy, N=5).
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PMC11750161
In the present study, eel LH/CGR-t651 mutant were completely impaired.
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PMC11021472
Exogenous expression levels of miR-150 in ST486 and DG75 upon infection with a lentiviral control vector (-) or miR-150 overexpression vector ( +). (
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PMC6684588
We next used surface plasmon resonance (SPR) biosensor methodology to characterize direct binding of ACE-083 to TGFβ superfamily ligands that negatively regulate skeletal muscle growth.
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