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OTAR3088
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BioData-CellLine_annotated

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PMC11599565
Notably, PAX6 accumulation was consistent across different cell lines.
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PMC11471176
For DMSO, it was four concentrations matching to those used with carnosine; namely, 0.0625 %, 0.125 %, 0.25 %, and 0.5 %.
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PMC10938336
Consequently, these findings offer valuable insights into the molecular basis of the compounds’ anticancer effects and pave the way for more targeted therapeutic strategies in cancer treatment.
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PMC7917457
Animals were sacrificed at day 41, and spleens and tumors were harvested for immunological analysis.
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PMC10114490
B GO molecular function enrichment within differentially expressed genes.
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PMC9429973
Aims: CELESTIMO (NCT04712097) is a randomized, multicenter, open-label Phase III study evaluating the efficacy and safety of M-Len versus rituximab plus Len (R-Len) in patients with previously treated R/R FL.
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PMC9429973
A. Abomhya, A. Razzaq, S. Lukose Internal Medicine, The Brooklyn Hospital Center, Brooklyn, United States of America; St. George’s University School of Medicine, West Indies, Grenada Background: Inflammatory bowel diseases include ulcerative colitis and Crohn’s disease and affect around 3.1 million adults in the United States (US).
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PMC11638764
When harvesting, cells were detached with TrypLE™ Express Enzyme (Gibco, cat# 12-604-021), washed with PBS (MilliporeSigma, cat# D8537) and spun down to collect cell pellets.
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PMC9119314
P. falciparum (K1 clone) was maintained in human erythrocytes prepared from the donated blood samples (blood groups unknown, Ethical Tissue, University of Bradford), suspended in the RPMI 1640 medium as previously described (Wright et al., 2001), except that serum was replaced with Albumax II, [5% (/v)].
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PMC11467964
PCR validation of PSAT1. (
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PMC11762280
Statistical analysis of epidermal thickness and average optical densities of TUNEL, MPO, MMP9, F4/80 positive staining were shown (n = 8).
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PMC11597167
SH-SY5Y cells were treated with rosmarinic acid, gallic acid, EGCG, oleuropein, 3-hydroxytyrosol, and quercetin for 24 h to determine their IC08 values, and RNA isolation was performed.
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PMC11696576
g Intracellular ROS measurements in CCRF-CEM cells treated with AVX420 (0.5 µM), AVX002 (8 µM), or AACOCF3 (8 µM) for 4 h. Data are the mean ± stdev of ≥3 biological replicates.
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PMC11126803
UALCAN database is mainly used for query genetic relationship with the prognosis of patients.
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PMC11583690
After temperature stabilization, 300 μL of 1,2-bis(triethoxysilyl)ethane was slowly added in a dropwise manner, followed by the addition of 100 μL of bis[3-(triethoxysilyl)propyl]tetrasulfide.
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PMC11394730
Cells require energy for survival, growth, and division, which is obtained through absorbing nutrients like glucose.
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PMC11779960
The degree of re-arrangement of the TCRβ locus may vary with different PTCL lymphoma subtypes and as Iyer et al. suggested, clonal evolution at a malignant cell level may further influence TCRβ usage.
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PMC11772449
After 48 h of culture, CCK-8 was used to detect cell survival and determine its proliferation activity.
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PMC11682172
Fig. 1BBB passage following co-culture with T cells or T cell medium.
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PMC11224020
Data were analyzed by ordinary one-way ANOVA; ****P < 0.0001.
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PMC10607604
HRP-labeled horse anti-mouse IgG (7076, Cell signaling technology) and HRP-labeled goat anti-rabbit IgG (7074, Cell signaling technology) were used as secondary antibodies for western blotting.
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PMC11772449
We used Western Blot to detect the expression of LC3B-II in hepatocellular carcinoma cell lines BEL-7404, PLC/PRF/5 and Huh-7 cells in the control group, Sora single drug group, pirfenidone single drug group and combined drug group.
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PMC6892818
a–c RCC cell lines ACHN, 786-O and A-704, the positive control EJ and normal HRPT cells were treated with mCD40L.
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PMC6190245
This investigation is the first to present the synergistic effects of F. gummosa and radiotherapy on Hela cells.
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PMC11764090
Sch.
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PMC11469524
We trained models for both cell lines on (a) the literature network (cf.
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PMC11694066
Next, the cells were filtered using a 70-μm cell strainer (#542070, Greiner Bio-One) and resuspended in complete organoid medium.
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PMC7090062
However, a study involving CAGE (a cancer/testis antigen)-expressing hepatoma and melanoma cells showed that miR-30a decreased the expression of p53 in a CAGE-dependent manner, leading to resistance to a HER-2 inhibitor, trastuzumab.
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PMC11459296
HLA-ABC-PE (G46-2.6, BD-Pharmingen) and CD45-PerCP-Cy5.5 (2D1, BD-Biosciences) antibodies were used to identify human immune-hematopoietic grafts (HLA-ABC+CD45+ population) weekly in PB and at endpoint from BM, liver and spleen.
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PMC9429973
Abnormal heterocellular adhesive interactions between RBCs and endothelial cells, leukocytes, and platelets play a central role in triggering painful vaso-occlusive crises (VOCs) in patients with SCD.
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PMC11578343
Using mz‐visco‐NPS, we examined the viscoelastic properties of human mammary epithelial cells that represented the entire continuum of epithelial transformation states: from average‐risk and BRCA1 mutant high‐risk primary epithelial cells (not cancer), to immortal non‐malignant, malignant, and metastatic cell lines.
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PMC11423992
Alterations in tumor volume were observed in the WIL2-S cell CDX model mice.
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PMC11653533
The ethanolic extract of F. racemosa leaves has manifested noteworthy antioxidant activity by FRAP (Ferric Reducing Antioxidant Power) assay, scavenging activity against H2O2 and TBARS (Thiobarbituric Acid Reactive Species) assay with values equal to 0.739 mmol Fe II/g, 51.28 ± 0.91 and 61.46 ± 0.92 % inhibition rate respectively (Yadav et al., 2019).
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PMC10965315
As shown in Figure 4a, treatment of A2780 cells with harmaline for 24 h resulted in an increase in ROS levels and oxidative damage to the cells.
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PMC10582049
Violin plots show expression of genes across identified stromal clusters. (
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PMC9429973
Key inclusion criteria: ≥2 PKLR mutant alleles with ≥1 missense mutation, ≤5 RBC transfusions in the past year, Hb concentrations of ≤10 g/dL (12 to <18 years) or ≤9 g/dL (1 to <12 years).
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PMC11519788
The total protein was used for normalization.
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PMC11545737
On the contrary, in the absence of Niap genes, the extrusion is reduced, and it is accompanied by Salmonella invasion of the epithelium in mice .
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PMC11461025
This indicates that A375 cells treated with isoginkgetin are undergoing apoptosis.
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PMC9429973
TNF levels were positively correlated with Lym (r = 0.30) and blasts (r = 0.40), suggesting a possible role for TNF in neoplastic clone survival.
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PMC10551595
This work was supported by an MSc research grant from the University of Isfahan (to M. K.).
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PMC9895440
DG inhibits glucose usage, thereby remaining ATP production measured by Puromycin incorporation is dependent on FAP/AAO.
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PMC11525028
We further conducted a flux assay across ASNS using N-labeled Asp (Figure 4T).
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PMC11679033
Data analysis, interpretation and reporting followed recommendations by OECD 2023 .
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PMC11720808
SCU inhibition of NF-κB activation is dependent on TLR4. (
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PMC11519583
In this study, we found that Ror2 expressed in MPs is critical for IMAT accumulation.
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PMC9429973
About 30% of patients (B-other-ALL) harbour none of the known major chromosomal changes.
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PMC11701801
A, Immunoblot analysis of CBP, p300, H3K27ac, H3, and β-actin expression in SWI/SNF-proficient, SMARCA4/SMARCA2-deficient, and SS18–SSX fusion cell lines transfected for 48 hours with the indicated siRNAs.
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PMC11578343
w p is designed to be two standard deviations greater than the mean cell diameter (Table S1, Supporting Information) to ensure that transiting cells do not experience an applied strain.
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PMC11545737
Different pyroptosis cascades occur, culminating in the activation of gasdermins and cell death.
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PMC9429973
TEAEs considered immune-related (IR) occurred in 38 (32.5%) pts.
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PMC9424261
We have shown that CA inhibits the synthesis of GSH by reducing GSS through HERPUD1.
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PMC11483468
The genomic DNA was extracted from cell by using FFPE DNA kits (Jiangsu Cowin Biotech Co., Ltd., Beijing, China), before diluting to 10 ng/µL. Then two pairs of primers (GC-Telc and GC-Telg, MRef1F and MRef1R) were added to each reaction system.
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PMC11795610
As cells undergo apoptosis, chromatin sequestraters.
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PMC11745823
Cell growth curves were evaluated using the CCK‐8 assay across different treatment groups, n = 3. (
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PMC11674891
These results suggest that GRPR-induced upregulation of LUAD cell invasion is mediated through AKT signaling.
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PMC11747467
To investigate whether inhibition of STAT3 could improve the anticancer effect of RC48, we first silenced STAT3 in T24 and 5637 cells ( Figure 5A ).
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PMC8303372
GA loading into NPs did not alter significantly their morphology, as can be deduced from Figure 1b and Figure 2a,b. The hydrodynamic diameters of empty NPs and GA-NPs were measured by DLS (Figure 3).
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PMC11676169
Forty hours after transfection, cells were lysed in modified NETN lysis buffer (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 1% Triton-X, and protease inhibitor cocktail).
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PMC9429973
Fixed duration I+V is hypothesized to decrease the likelihood of developing resistance mutations and facilitate extended clinical benefit.
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PMC11627060
The drugs exhibit side effects and have a short duration of action within the body .
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PMC9621239
G-K) All clones of the HBV X gene isolated from patients 4, 11, 12, 13 and 23 were genotyped using the phylogenetic trees.
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PMC10197932
MOLM-13 has a high loss of functional mitochondrial already at one treatment (T) (Figure 5.3 C).
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PMC11248911
Compound 8 showed an IC50 value of 29.1 μM, exhibiting a selectivity index equals 4.7, Table 1.
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PMC11790971
To account for imbalanced datasets, balanced accuracy () was used, which is defined as:7[12pt] $$=_^_+_}}$$=∑i=1Csensitivityi+specificityi2Cwhere is the number of classes ( in this study).
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PMC11674146
For the second part, BJAB and RA 1 cells were seeded in 96-well plates at the density of 3 × 10 cells/well for overnight culture, followed by the incubation with 50 μM 5-ethynyl-2′-deoxyuridine (EdU, E796036; Macklin, Shanghai, China) reagent at 37 °C for 1 h. Further, DAPI solution (C0065; Solarbio LifeSciences, Beijing, China) at 0.1 μg/mL was adopted for nuclei dyeing for 20 min at room temperature and the results were finally observed in a fluorescence microscope (DM6B; Leica Microsystems, Wetzlar, Germany) (Wang et al., 2022).
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PMC9672323
The graphs show the mean ± SD.
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PMC8010066
No stars: the non-statistical correlations The two-way ANOVA test and Tukey's data analysis indicated a unique relationship between co-culture and antibiotic susceptibility (P-value ≤ 0.001).
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PMC9100622
All experiments were performed in duplicate, in 3 independent experiments.
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PMC3765139
Frequency of proliferating cells was expressed as mean ± SD.
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PMC11562028
At the same time, the Hoechst33342/PI staining showed that the intracellular chromatin of the A498 was concentrated and brightened, compared with the control group.
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PMC11244823
Starting from the N-terminus of gD1, we developed luciferase immunoprecipitation system (LIPS) assays, using gD1-fragments of increasing size as antigens, i.e. gD1_83 (comprising the first 83 amino acids), gD1_160, gD1_180, and gD1_402 (the full-length molecule).
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PMC6461034
Add the wash to the tube with eluate 1 and eluate 2 and mix.
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PMC11462673
F Flow cytometry using Annexin V staining showed that knockdown of LDB1 increased the apoptotic rates of Jurkat, 6 T-CEM and J.gamma1 cell lines.
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PMC7338939
q-RT-PCR analysis of stemness-related genes showed that 48 h treatment with DAPT enhanced the expression of NANOG, OCT4, and NESTIN in adherent culture.
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PMC11718809
The top 20 MF items include molecular_function, binding, protein binding, etc. (
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PMC9429973
Methods: TPE was performed in a 72-year-old patient necessitating emergency surgery for a voluminous uterine sarcoma in the acute phase of HIT (10 days after diagnosis).
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PMC11442172
At that time, Farber and colleagues demonstrated that aminopterin, a folate analogue blocking de novo nucleotide biosynthesis, could halt tumor progression in children with acute lymphoblastic leukemia (ALL) (6).
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PMC8735881
The bifunctional constructs also provide new arsenals fighting HIV-2, which has already caused increased infections worldwide .
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PMC11628904
Data are normalised to photoconverted area 0 hpb.
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PMC11708541
Cancer is the leading causes of premature deaths globally.
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PMC11656380
Nonapoptotic cell death mechanisms, such as pyroptosis, necroptosis, ferroptosis, NETosis, and lysosome-dependent cell death, often lead to plasma membrane rupture and the release of inflammatory molecules.
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PMC11411131
In Krab-Sec 2 or Krab-Sec 3, Sec is positioned at the + 7 or + 121 position of TetR-Krab.
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PMC11725127
In the present study, we demonstrated that USP2 is a DUB that directly controls KRAS ubiquitination, as supported by the following evidence: 1) overexpression of USP2 stabilizes KRAS; 2) USP2 interacts with KRAS in cells as indicated by the immunoprecipitation and immunofluorescence assays; 3) USP2 directly removes ubiquitin from ubiquitylated KRAS, which leads to KRAS stabilization, whereas USP2 inhibition results in the proteasomal degradation of KRAS.
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PMC8998437
Expression of the CCR1 protein (CD191) on the cell surface and percentages (%) of CD191-positive (CD191+) and CD10-positive (CD10+) cells were determined using flow cytometry; annexin V-stained (apoptotic) cells were excluded from the analysis.
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PMC11742670
Feature selection has garnered significant attention with the objective of choosing highly relevant features starting with unprocessed datasets to enhance how well a learning model performs.
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PMC11774112
Tumor weights of mice receiving different therapeutic regimens (n =6, mean ± SD). (
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PMC11388384
Additionally, whereas TIRF imaging captured SHH-Halo at a discrete moment during gradient formation, live cell signaling reporters integrated the SHH concentration experienced during ~48 h of SHH signaling, and so are likely to be more sensitive to low amounts of SHH compared to TIRF-based imaging.
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PMC2777936
The Topflash lentiviral vector was obtained from Dr. Karl Willert (University of California, San Diego).
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PMC11786600
Material science experiments have shown that the dressing has optimal fiber micromorphology and good water vapor transport properties (WVTR: 4.88 kg m 24h); in vivo, diabetic wound experiments have shown that the high porosity and pharmacological effects of PED4 can mutually promote the rapid healing of diabetic wounds (healed 95.9% on day 15), facilitate the transformation of macrophages from M1-type to M2-type and regulate the expression of MMP9.
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PMC9429973
Overall, this review of the TBMTR provides a snapshot of the current transplant state and highlights important HCT trends in Taiwan.
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PMC10890307
In this phenomenon, CAFs, via aerobic glycolysis, provide the necessary fuels (l-lactate, ketones, amino acids, and fatty acids) to tumor cells .
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PMC8524093
Circulating virus was observed until 15 dpi in 20-week-old C57BL/6 mice challenged with 2 × 10 pfu/ml by subcutaneous route in the hind limbs (Jain et al., 2019).
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PMC11655536
C Immunofluorescence of the TFE3 in the nucleus in RPMI-8226 (upper) and U266 (lower) cells when treated with HA at 10 μM for 48 h. D Western Blot of the expression levels of TFE3 when infected with lentivirus carrying shTFE3 in RPMI-8226 and U266.
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PMC6600253
These compounds showed great anticancer effects and inhibited cancer cell growth via the induction of apoptosis.
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PMC11438317
After PV treatment for 5 min, the phosphatase domain of LYP was immunoprecipitated with FLAG antibody bound to Sepharose beads, and proteins present in the precipitates were identified by Western Blot.
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PMC10073922
Nonetheless, LX-2 is a hepatic stellate cell line and the lack of evaluation of Sur-P activity in the lung epithelial cells is among the limitations of this study.
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PMC9512971
The primary endpoint in that study was a composite of the time to discharge or a clinical improvement in clinical severity of at least 2 points on a 7-point ordinal scale.
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PMC11704135
One of the primary modes of action of pyrimidine derivatives is their ability to inhibit the activity of thymidylate synthase (TS), an enzyme involved in DNA synthesis.
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PMC10243817
Dual luciferase assay results showed that depletion of the 4106 to 5564 (F3) segment in the 3′ UTR abolished the inhibitory effect of MEX3B on the luciferase activity (Figure 3D).
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