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OTAR3088
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PMC9429973
Results: Our analysis identified 7 lncRNAs significantly upregulated in MF patients’ plasma compared to HDs.
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PMC11144200
Mice were randomly assigned to a total of six categories (5 mice per category) and given the appropriate therapy (IR-780 at a dosage of 0.1 mg/ml) when their tumors grew to a size of about 100 mm.
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PMC11597291
Moreover, the hydrophobic interactions between the pristine nCGO lattice and the phospholipids on the RBCs membrane may lead to the damage and disruption of the cellular membrane .
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PMC11604768
The activation of μOR with the agonist morphine resulted in just two promoters with FDR values below 5%, in line with our results for D1R, suggesting that activation of Gαi-coupled GPCRs does not regulate cellular transcription in HEK293 cells (Supplementary Fig. 9A).Fig.
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PMC9429973
Orca-T exhibited GRFS of 71% & OS of 90% at 1 year.
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PMC11746948
In Fig. 4k, the box plot is presented to illustrate the correlation between the sensitivity of [860 cancer cell lines to 481 compounds] and [different levels of ZDHHC20 gene expression].
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PMC9473868
Recently, studies have also shown that BAI can treat osteoarthritis (OA) by inhibiting chondrocyte apoptosis, reducing cartilage damage, and reducing synovitis, suggesting that BAI has the potential to slow the pathological progression of OA .
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PMC11476264
Cells (5 × 10) were plated in the top chamber and incubated at 37 °C overnight.
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PMC11791478
The method measured total 25A3 by capturing it with a biotinylated recombinant His-tagged human TF (TF-His) on Meso Scale Discovery streptavidin plates and detection with ruthenium-labeled anti–human IgG. The assay quantifiable range in the neat matrix was 76 to 80,000 ng/mL in an exploratory study and 200 to 80,000 ng/mL in a toxicology study.
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PMC5673955
5 μg of total RNA was reverse‐transcribed using RevertAid RT Kit (Invitrogen) in a 25‐μL reaction mixture followed by real‐time RT‐PCR using CFX96 Touch Real‐Time PCR Detection System (Bio‐Rad, Hercules, CA).
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PMC11637284
The final eluate was concentrated using a 3 kDa size filter (Millipore) to approximately 1 μg/μl as determined by the BCA test (Thermo Scientific) .
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PMC9429973
The aggressive B-NHL classifier was validated on a cohort of 35 cases, including 14 DHL cases (diagnosed by FISH) and 21 DLBCL cases.
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PMC11745600
Homozygous Akita mice (Ins2) cannot be used for breeding for strain maintenance because of male infertility.
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PMC11731614
As expected, we observed a strong GFP signal along the outer membrane of these keratinocytes (Fig. 5D).
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PMC9429973
Methods: Pts enrolled were ≥18 years with histologically confirmed CD20-positive FL, R/R to any prior rituximab-containing regimen, ECOG PS 0–2, ≥1 measurable lesion, and ≤4 prior therapies.
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PMC11742290
High expression of LGR5 in ovarian cancer cells was associated with resistance to carboplatin .
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PMC11670954
Compared with LPS treatment alone, BA significantly mitigated the reduction in the TEER, decreased FD-4 flux permeability, increased the mRNA expression of ZO-1 and Occludin, and normalized the distribution of ZO-1 and Occludin in Caco2 cells.
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PMC11757230
When separated from cADSCs, cell death in CLC cells increased compared to conditions with intercellular contact, while the proportion of cells in the G2/M phase increased, and the proliferation-inhibitory effect of cADSCs was lost.
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PMC11730311
hαCGRP8-37N25K-C20DA-γGlu was therefore selected as backbone for further optimization.
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PMC9429973
Six pts had FLT3 mutations and 4 pts CBL mutations.
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PMC9429973
All study drugs were administered by IV infusion on Days 1 and 15 of each 28-day cycle.
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PMC9878278
SC were transfected with either scrambled control (siCtrl) or (A) TLR3 or (B) TLR7 specific siRNA and TLR3 and TLR7 expression was measured at 48 h post-transfection (hpt) by RT-PCR to determine silencing efficiency.
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PMC9429973
iMR was achieved in 54% patients.
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PMC11726183
Absorption, distribution, metabolism, and excretion Triple negative breast cancer Breast cancer The United States Food and Drug Administration Fourier Transform Infrared Spectroscopy Nuclear Magnetic Resonance High-resolution mass spectrometry Human epidermal growth factor receptor 2 Half-maximal inhibitory concentration Root Mean Square Deviation Root Mean Square Fluctuation Molecular Dynamics Simulation Molecular mechanics with generalized born and surface area solvation The data used for the manuscript entitled “Design, synthesis, in vitro, and in silico studies of novel isatin-hybrid hydrazones as potential triple-negative breast cancer agents” will be included in an ESI file,† available online on RSC Advances web site.
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PMC3122064
Fascinatingly, blocking of the Fas-FasL interaction in TC cells impairs apoptosis induction by Nutlin-3 as well as combined treatment with Nutlin-3 and cisplatin.
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PMC11531744
We then intersected all differentially expressed genes (DEGs) with immune genes to arrive at all of the immune DEGs in OC.
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PMC11422497
The cell lines were cultured in Dulbecco’s minimum essential medium supplemented with 10% fetal calf serum at 37 °C in a humidified atmosphere containing 5% CO2.
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PMC10748106
These molecular descriptors and structural features affect the reactivity of the simple iridoids, particularly the substituent at C4 (aldehyde or ester), added to the forward hydroxyl at C8.
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PMC11609529
The YM fusion protein consistently displayed nuclear speckles, with the number of speckles varying between cells (Figure 2C, top).
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PMC7723249
Cells were incubated for 48h (37°C, 5% CO2) and thereafter fixed in ice cold methanol/aceton (1:1) at -20°C for 24h.
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PMC11714165
As depicted in Figure 5B,C, the tumor growth was rapidly progressive in the PBS and the NBD groups.
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PMC11766309
According to the cBioPortal database, YKT6 amplification was shown to be the most common type of YKT6 gene mutation across cancers.
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PMC9429973
We hypothesized that distinct phenotypes of PBMCs from HI and NI exist and that specific subsets of mononuclear cells promote EBV-LPD in this model.
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PMC11683240
The bar graph (E) shows the quantifications of Western blot signals from seven independent experiments, while (F and G) show blots of representative experiments.
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PMC11787381
One-way ANOVA followed by Fisher’s LSD test. *
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PMC11752740
According to the Chou-Talalay method, the CI value for each fraction of cells affected by a cytotoxic agent (termed Fraction affected or Fa) is calculated using the equation : CI = (D₁)/ (Dx)₁ + (D₂)/ (Dx)₂. In this equation, D₁ and D₂ represent the concentrations of each drug when administered individually, while (Dx)₁ and (Dx)₂ denote their concentrations when combined.
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PMC11762280
F Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL in the epidermis of Ac-DEVD-CHO or PBS treated mice were detected by western blotting assay.
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PMC11015054
P ＜ 0.05, **P ＜ 0.01, ***P ＜ 0.001.
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PMC10976516
Adjuvant cisplatin-based chemotherapy is the gold standard of care for some stage I and the majority of stage II and III patients with completely resected NSCLC.
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PMC10547921
Compound 11 had an IC50 of 6.0 nmol/L on H3396 cells and compound 12 had an IC50 of 0.09 nmol/L on HCT116 cells.
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PMC11224020
g, Heat map showing normalized expression of some IFN-stimulated genes in the CD11b cDC2 subset from the dermis and their migratory counterparts in the cutaneous draining lymph node of healthy mice (GSE49358 microarray study from Tamoutounour et al.).
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PMC11803149
Analysis of exhaustion-related molecules revealed no induction of PD-1 and only minimum induction of Tim3 and Tox in aged TCR-T cells ( Figure 1E ).
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PMC11782508
Approximately 10 mg of the lyophilized, purified azurin fraction was finely ground with potassium bromide (KBr) crystals.
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PMC11792090
The CAR-T cells were irradiated and labeled with CFSE.
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PMC11703992
Here, we conducted transcriptome analysis and utilized the mendelian randomization (MR) method to examine and validate the causal association between genes linked to CD4+ Memory T cells and occurrence of GCA.
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PMC11337594
In this study, we observed that the overexpression of BAX and GSDME markedly enhanced LDH release in A375 cells, which was effectively attenuated by the introduction of a pan-caspase inhibitor.
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PMC11451829
Additionally, the microscopic characteristic of lymphocytic infiltration, with the presence of plasma cells reported by Hill and Maske, is present in our pterygium-like lesions.
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PMC8540692
The cytotoxic activity of CCM, NaBu, and their combinations, as well as Aza, a demethylating agent used as a control for methylation analysis, was performed using the MTT assay.
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PMC11033180
To define synapsin 1 clusters, a threshold was set to be two times higher than the underlying fluorescence intensity of the axonal processes.
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PMC11626627
In addition, studies have reported that TWIST1 is one of the key regulatory factors mediating EMT.
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PMC9429973
Materials: The number of all transfused pts and units of blood components in the Specialized Hospital for Active Therapy of Hematological Diseases for the 2020– 2021 period were: pts–2,521 adults (665 transfused only with red cell concentrates–RCCs,98 of whom with Beta Thalassemia); all blood components(units)–20,040, of which –9,743 RCCs, 3,084 platelet concentrates by apheresis (PCAP), 5,090 PC from whole blood(PCWB), 2,099 fresh frozen plasma(FFP), (severe clinical cases with post-transfusion complications–delayed hemolytic transfusion reactions, anaphylactic reactions–will be outlined), alloimmunization from RCCs–26 pts.
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PMC11770199
The syncytia appeared at 8 h after transfection and continued to fuse with neighboring cells over time.
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PMC11033180
Therefore, the establishment and maintenance of the axonal arbor of enteric neurons are crucial mechanisms underlying gut motility.
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PMC11544122
The WT response from panel (b) is superimposed in panel (c) (dotted line).
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PMC8303372
In the second condition, NPs were lysed by chloroform and therefore their GA content was totally released in the test tube.
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PMC6024716
The next step would be to investigate the effect of the OAZ1 deletion in cells growing in suspension, which are more relevant to large-scale recombinant protein production.
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PMC6190245
As shown in Figure 2, at low concentrations (3-30 µg/ml), F. gummosa did not show any significant cytotoxicity in HeLa cells.
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PMC10988808
Data are presented as mean ± SD. *
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PMC10818573
Meanwhile, the RPA reaction system lacking a B95-8 DNA template was supplemented with different components (probe, nfo, and nfo buffer) in turn.
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PMC11297139
To account for both proximal and long-range gene–cRE interactions, we considered each gene–cRE pair as linked if they are co-localized (<40 kb) or if chromatin contact significantly increased between the two elements upon the LLPS of ARID1A in 40 kb resolution.
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PMC11700165
ITK-SYK was recurrently identified in a subset of peripheral T cell lymphomas, while translocation leading to TEL-SYK has been documented in 2 patients with myelodysplastic syndrome.
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PMC9622879
The SEHS was an infrastructure comprised of all communal DERs and different types of customers (homes) of the community.
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PMC11228511
We also used live cell imaging to investigate the relationship between NM ruptures and blebs.
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PMC11725127
Briefly, the cells were cultured in 96-well plates, treated with drugs at the indicated concentrations, and then incubated with the CCK-8 working solution for 1–4 h at 37 °C.
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PMC4270159
Serum starve = DMEM + 0% FBS.ii.
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PMC10993311
BMI: Body Mass Index.
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PMC11471176
The reaction was stopped by adding 50 μL of stop solution to each well, resulting in a color change from blue to yellow.
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PMC11228511
Red circles in bar graphs show the average values of independent experiments or values for individual animals.
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PMC10996034
The results revealed that only UNC5C was significantly associated with the survival of patients with HNSC treated with Taxol.
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PMC11322866
Therefore, we explored whether p-ERK1/2 is involved in EZH2-induced senescence in MM cells.
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PMC11750712
The study consists of two phases: phase 1 dose escalation and phase 2 dose expansion.
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PMC11770746
In vivo behavioral studies. (
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PMC11342785
For making the OptoTPPP, different TPPP fragment were produced by PCR then cloned into the pHR-mCh-Cry2WT plasmid.
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PMC11754784
n = 3 from three independent experiments).
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PMC11736898
Absorbance values are proportional to cell number, with higher values reflecting greater proliferation levels.
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PMC7570809
Cell lysates were incubated on ice for 30 min and then centrifuged at 13.000 rpm for 30 min to remove cell debris.
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PMC9429973
The Energetics hallmark has not, to our knowledge, been linked with AML prognosis before, and may suggest novel biology linked to treatment response.
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PMC11594074
Notably, compound 2 did not show considerable cytotoxicity toward any selected cell lines.
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PMC10237474
For a dual-expression vector transfection, 1.5 μg of each chain and 3.0 μg of PB were used.
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PMC10213179
We next examined the functional effect of ATOP aptamer internalization on the clonogenicity and migration capability of several tumor cell lines from different tumor types, including ES, ARMS, or triple-negative breast cancer (TNBC) that overexpress EphA2 membrane receptor (Figure S2A).
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PMC11082662
Our experiments also revealed that ACER2 knockdown inhibited ovarian cancer, potentially due to the high expression level of SMPDL3B induced by increasing cancer cell proliferation, migration, and invasion.
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PMC11615218
Consistently, recent research has indicated that CAR-T cells maintained at a lower range of normal pH at the beginning of the manufacturing process show enhanced T-cell proliferation and reduced glycolysis .
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PMC11486946
For the other compounds, EGFR was observed at 488 nm excitation (see “Methods”).
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PMC11768281
These stages can be categorized into three phases: pre-activation, activation, and post-activation.
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PMC11739504
Interestingly, the proportion of Kat2b localized in the cytosol and centrosomes slightly increased 6 h after serum starvation.
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PMC11633763
Three enzymes—ubiquitin ligase (E3), ubiquitin activating enzyme (E1) and ubiquitin binding enzyme (E2)—are involved the ubiquitination reaction.
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PMC11711935
Similar to keratinocytes, fibroblasts release various cytokines to regulate melanocyte activity, such as stem cell factor (SCF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and basic fibroblast growth factor (bFGF) , which are known to promote the growth, survival, and melanogenesis of melanocytes.
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PMC11604015
In contrast, allogeneic CAR-T cells are of high purity and quality, but allogeneic adoptive infusion has limitations, such as GvHD and allo-rejection .
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PMC9429973
The following significant predictors of hospital mortality have been identified: age >65 years, relapse/disease progression, CT severity score index of 4, grade 4 neutropenia.
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PMC10454535
To compare the potencies of the RES derivatives, IC50 values were calculated from the individual compound viability data (Table 1).
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PMC11715644
The data supporting this article have been included as part of the ESI.† Source code for analysis of collective cell migration (MATLAB license required) are freely available at https://github.com/sahuyu/SWS-Post-Processing.git The authors declare no competing interests.
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PMC11437637
Sample characteristics are expressed as x̄±s or M (P25, P75).
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PMC9429973
.
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PMC11659734
This migration can occur under certain conditions, such as high temperatures or prolonged storage times, leading to BPA exposure in humans (Rochester & Diamanti‐Kandarakis, 2013).
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PMC11075223
The recent research progress of Cimicifugae Rhizoma in ethnopharmacology, phytochemistry and pharmacological effects demonstrates the effectiveness of its utilization and supplies valuable guidance for further research.
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PMC9429973
1 died with progressive disease.
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PMC11733919
Then, the samples were diluted up to 300 μL with miliQ water.
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PMC11591038
For example, HIF-1α is critical for the differentiation of various T cells including Th1, Th17, and CD8-positive effector cells; HIF-1 may also inhibit the development of regulatory T cells .
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PMC8864075
Actually, tumor cells obtain energy mainly through the process of glycolysis, which promotes the EMT process of tumor cells (Liberti and Locasale, 2016); when nutrients were depleted, cancer cells tend to obtain metabolic materials through the pentose phosphate pathway (Georgakopoulos-Soares et al., 2020).
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PMC10671321
HiPSC was generated by the core facility SAFE-iPS at the Institute for Regenerative Medecine and Biotherapy (Montpellier, France).
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