PMCID
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Sentences
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PMC11754436
Identification of key functional molecules in OS is urgently required to develop effective preventive measures and reasonable treatment methods for OS.
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PMC3765139
Furthermore, during a suggested metastatic transition, originally CD133- induced tumors can transform to CD133+ xenografts.
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PMC11720107
A notable observation was that the strong decrease in CAT mRNA expression caused by the Ornak cultivar was accompanied by an increase in CAT protein expression.
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PMC4005030
It is also a very common compound present in human diet, present in bran and grain brown rice (Oryza sativa L.) and onion (Allium cepa L.) , especially in the scales.
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PMC9429973
2021 Aug;84:110012.
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PMC11754094
DNA sequences (10 kb) are amplified from genomic DNA isolated from CRISPR-treated and non-treated cells.
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PMC11478724
RNA extraction electrophoresis gel (1%) from A549 with and without PMX-treated after DNase I treatment (a).
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PMC11763126
Both culture media were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and cells were incubated at in a 5% CO2 environment.
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PMC10452486
Kollnberger’s team assessed KIR binding to HLA-Ia variants using several approaches, which included (i) flow cytometric analysis of KIR-expressing cell lines stained with HLA-I Face-1; (ii) recombinant KIR Fc fusion proteins or KIR tetramers coated fluorescent multiplex beads; (iii) physical measurements of KIR binding to HLA-I variants; (iv) binding of KIR-expressing reporter cells to HLA-I variants or to HLA-I variant transfected cell lines; and (v) KIR inhibition of NK cytotoxicity and IFN-γ production by binding to HLA-I variants.
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PMC10452486
We hope that our hypothesis may stimulate further studies on the multivarious HLA-I antibodies formed against novel HLA-I heterodimers in mismatched organ transplantation, which may play a role in transplant rejection.
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PMC10490530
Reporter genes are also extensively used for performing non-invasive imaging procedures in various vertebrate, invertebrate, and plant model systems .
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PMC11746948
Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific, USA).
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PMC10530622
Pyridoxine (VB6), a AGT cofactor, can partially rescue mistargeting by stabilizing the protein.
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PMC9429973
The diagnosis of APL is based on morphological, cytogenetic and molecular study.
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PMC11345828
The title compound (108 mg, 69% yield) was prepared following General Procedure F using compound 60 (150 mg, 0.19 mmol, 1.0 equiv) and (R)-tetrahydrofuran-3-yl 4-methylbenzenesulfonate (135 mg, 0.56 mmol, 3.0 equiv) followed by saponification using General Procedure G. LCMS Method 1: RT = 2.203 min, MS (ESI): mass calcd for C45H47Cl2N5O7, 839.3, m/z found 839.8 (M + H).
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PMC10440586
The x-axis represents the time interval between the first and the second surgery. (
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PMC11525028
Mechanistically, NAT10 stabilizes ATF4 mRNA and inhibits ATF4 mRNA degradation by ac4C modification.
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PMC11129910
Metaphase chromosomes from HMEC 5.6 RAB25 were prepared by using standard cytogenetic techniques.
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PMC9454178
C Colony formation assays of GIST-T1 and GIST-882 cells after CDK7 knockdown.
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PMC11638288
The study also employed Ig fusion proteins made from the D1 domain of NKp46, which allowed for the observation of specific, dose-dependent binding of NCR1 Ig and NKp46 Ig to F. nucleatum, indicating a direct interaction between NCR1 and NKp46 with F. nucleatum.
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PMC9841531
Amongst the high-throughput screening-(HTS)- and organic synthesis-derived compounds, only a few structurally distinctive compound classes can be differentiated: (i) thieno[2,3-d]pyrimidin-2,4-diones, [e.g., AZD-3965 (3)], pteridine derivatives (e.g., compound 4(33)), pyrrolo[3,4-d]pyridazinones (e.g., compound 5(29)), coumarin derivatives [e.g., 7ACC2 (6)], the sulfonyldibenzene BAY-8002 (7), as well as cinnamic acid derivatives [e.g., hydroxy-4-cyanocinnamic acid (CHC); 8].Figure 1 shows the molecular structures of compounds 1–8.
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PMC9918897
After in vitro cultivation, 10⁷ living HeLa cells (ATCC No.: CRM-CCL-2, BSL 2) were suspended in 100 microliters of physiological saline.
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PMC11637276
Our data suggested that MAPK signalling regulates ECM endocytosis (S2 Table).
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PMC11617590
PDAC with low Ser9 phosphorylation of GSK‐3β inflicts a negative prognosis owing to sustained tumor‐promoting signals .
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PMC11286266
HEK293T cells (ATCC CRL-3216) were cultured in Dulbecco's Modified Eagle Medium (DMEM) cell media (Sigma) supplemented as above.
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PMC11687663
The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in Acetonitrile HPLC grade (B) at a flow rate of 1.2 mL/min.
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PMC10761571
Therefore, we aimed to design Fe3O4 NPs functionalized with glucose and conjugated with gingerol, as a therapeutic component.
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PMC9429973
A 34.5 % of the patients were fully vaccinated.
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PMC6190245
Comparison of the cytotoxic effect of different concentrations of Ferula gummosa and its radiosentisizing effects on HeLa cells.
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PMC11116779
Each value represents the mean ± SD of six independent experiments.
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PMC11075223
Ohwi .
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PMC11264242
A further examination of the peak distance to transcription start site (TSS) revealed that FLAG-tagged SS18-SSX2 primarily occupies genomic regions close to the TSS (Figure 1A).
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PMC11803918
Subsequently, cells were permeabilized with 0.2% Triton X-100 and blocked in 3% BSA in PBS for 1 h at room temperature.
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PMC11424574
Using TNMplot software to screen the TCGA database, we observed that uPA expression was elevated in human osteosarcoma tissues compared to that of normal tissues (P = 1.13e) (Figure 3D).
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PMC11026382
a Volcano plot examining the statistical significance of epistasis across all DHFR point mutations in the Q33S background.
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PMC11525028
Osteosarcoma cell 143B stably expressing green fluorescent protein (GFP) were generated and validated for functional screening.
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PMC9261184
The researchers boarded the ship on March 25, 2021, while the ship was anchored off the port of Corpus Christi, USA.
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PMC11348300
In a similar way of how perfusion-based ICG imaging has gained prominence for intraoperative tissue assessment, imaging DF and quantifying PRESTO contrast is a promising method for assessing functional intra- an extracellular differences between cancerous and healthy cells.
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PMC11400680
Specifically, points, boxes, and texts are termed as sparse prompts while masks are referred to as dense prompts.
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PMC11345828
The combination of 26 and docetaxel (60 mg/kg + 10 mg/kg, q7d) resulted in a deepened response relative to either single agent treatment, with no sign of outgrowth after 21 days.
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PMC8973085
; IR (KBr, v, cm): 3446 (NH), 3336 (NH), 2225 (CN), 1661 (C=O), 1641 (C=O); H NMR (DMSO-d6): δ 2.36 (s, 3H, CH3), 2.54 (s, 3H, CH3), 4.15 (s, 2H, CH2), 7.11–7.97 (m, 12H, CH-Ar), 9.46 (bs, 1H, NH), 10.63 (bs, 1H, NH); MS m/z (%): 528 [M] (95).
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PMC10529414
A549 is a differentiated-type adenocarcinoma cell line derived from lung tumor tissue of a 58-year-old Caucasian man .
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PMC9429973
Given the minimal response rates at all dose levels, the phase II expansion portion of the trial was not conducted.
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PMC11766309
A negative correlation was observed between the expression of YKT6 and the infiltration of numerous types of immune cells in ESCA, HNSC, STAD, THCA, and UCS.
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PMC11723947
Prior to adoptive transfer into NSG hosts, human Treg were first cultured in ImmunoCult-XF T Cell Expansion Medium (Stemcell) containing ImmunoCult Human CD3/CD28/CD2 T Cell Activator (Stemcell), 500 IU/mL rhIL-2 and 100 ng/mL Rapamycin (Sigma).
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PMC11640115
These factors influence neutrophil counts and may be related to the weak effectiveness of the NLR compared to the imGPS in correctly predicting pembrolizumab efficacy.
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PMC8068766
Se: Selenium-deficiency effect, HBV: HBV-integration effect, BCL: Between cell line, WCL: Within cell line, (E): Existing DEG, (S): Steiner node, HM: Heat map, OS: Oxidative stress, r: Reported.
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PMC11755519
EVs can activate receptors or alter miRNA or RNA expression in neighboring tumor cells causing, for example, an increase in their migratory, invasive, or proliferation potential.
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PMC11607321
Features ranked by their reconstruction quality are shown for the drug response (left) and the CRISPR-Cas9 (right) datasets.
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PMC9429973
The ASCEND trial compared acalabrutinib with investigator’s choice chemotherapy, either idelalisib plus rituximab or bendamustine plus rituximab.
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PMC11252553
The antioxidant activity of Taurine is believed to be mediated through various mechanisms, although the exact mechanisms are still under investigation.
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PMC11453018
This oncogenic protein modulates immune-related gene expression, alters cytokine profiles to favor immunosuppression, interferes with effective antigen presentation by downregulating MHC class I molecules, and manipulates the tumor microenvironment to encourage the recruitment of immunosuppressive cells like M2 macrophages .
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PMC11609529
Our study addresses this gap by showing the endogenous YM fusion’s expression, its interaction with the TEAD transcription factors, and its essential role in supporting cancer cell proliferation and survival.
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PMC9429973
Aims: Here, we analyze the interplay between BH3 protein family members in PI resistant vs. sensitive cell lines aiming to decipher survival strategies of PI resistance.
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PMC11670407
Mechanistically, GPX4 could be a downstream target of OTULIN, conferring cisplatin resistance to OS by blocking the mitochondrial apoptotic pathway but not ferroptosis.
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PMC8864075
As our research provides direct evidence, TWIST1-targeted flavonoid may provide a new strategy to inhibit EMT progress.
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PMC11397654
The body weight of the mice was monitored throughout the experiment and was determined to be constant, indicating that the nanoplatforms are safe and cause relatively insignificant initial side effects.
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PMC11750161
To construct point mutations in eel LH/CGR activating and inactivating mutants, we used cDNA encoding full-length eel LH/CGR-wt using an overlap extension PCR strategy to substitute specific amino acids, as described previously (Byambaragchaa et al., 2021b).
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PMC6114978
To determine more directly whether promoter hypermethylation and histone deacetylation work in conjunction with one another to regulate expression of the Hippo kinases, we treated cells with 5 μM 5-azacytidine (AZA) for 4 days followed by treatment with 500 nM trichostatin A (inhibits class I, II, and IV histone deactylases (HDACs) by interacting with the Zn(II) in the catalytic site ) for 12 h (Figure 6A–6D).
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PMC11063646
The pH of the optimized emulgel (ANG 12) was found to be 6.4 ± 0.5 indicating that the formulated emulgel is deemed safe for skin application.
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PMC5415764
Using high-throughput or systems biology methods like metabonomics would hold excellent prospect for tumor treatment research in the future.
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PMC3164356
Many of these genes are either cell adhesion molecules (CAMs), important for viral entry and vesicular trafficking, or plasma membrane proteins involved in viral recognition.
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PMC11087055
Thus, the large-scale cell flow and midline morphogenesis in amniote gastrulation is not tightly coupled, which may be evolutionarily distinct from non-amniote gastrulation.
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PMC11519788
Meanwhile, sphere formation assays indicated that U937 cells led to a significant increase in both the number and size of mCherry-labelled T24 spheres, whereas SPOP overexpression effectively mitigated this U937-induced enhancement (Figure 3B-C).
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PMC9429973
Tocilizumab and corticosteroids were given to 92% and 86% of pts with both CRS and ICANS.
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PMC11354239
The down-regulation of gene expression appeared to be the main transcriptional change detected in degranulating responder NK cells compared to non-responders.
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PMC9429973
Median age was 57 years (range 20-92).
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PMC10329237
A study reported that angiotensin II type 1 receptor blockers could postpone the damages induced by free radicals through increased SOD levels while decreasing MDA contents (13).
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PMC11594074
A large chemical library containing approximately 10,000 compounds from the ZINC15 clean subset, as well as 1500 FDA-approved drugs, was established.
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PMC8414691
In contrast, SRCAP and DOM-A depletion leads to specific categories of mitosis and cytokinesis alterations.
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PMC11679033
Industrial hemp (C. sativa L. subsp.
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PMC9727330
The number of surviving cells significantly decreased in response to NNDAsp treatment only in colon cancer cells.
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PMC11597291
The nCGO-PEG conjugates were characterized by Fourier transform infrared spectra (FTIR) obtained from 600 to 4000 cm using a DigiLab Excalibur series FTS 3000 spectrometer (Labexchange, Burladingen, Germany) equipped with an attenuated total reflectance (ATR) and a class II laser.
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PMC9429973
Patients without HR CA had a lower PFS and OS when they carried del(1p32) (del(1p32) vs. no del(1p32); PFS 25.6 vs. 34.8 months, P = 0.0004; OS 83.0 vs. 136.1 months, P = 0.0002).
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PMC11481779
Fig. 1FID score of the real and augmented data - example of chr8 8 600 000–10 600 000.
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PMC9429973
Interim analyses will be performed annually; an effectiveness interim analysis is scheduled at 20 months post-LPFV.
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PMC11802929
a Graphic relating the SARS-CoV-2 proteome to putative HLA A*02:01 restricted antigens (top row), to those SCT constructs that were expressed in usable yield (middle row), and those that captured antigen-specific CD8 T cells from COVID-19 participants (bottom row).
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PMC8973725
Unequivocal nuclear and cytoplasmic staining patterns of ZNF677 were considered positive.
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PMC10047551
The DNA methylation of cg19724470 and cg(chr9:5450951) was not significantly correlated with PD-L1 mRNA expression, although there was a trend of inverse correlation between cg19724470 and PD-L1 mRNA expression (spearman r = −0.33, p = 0.0906) (Figure 5A).
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PMC10698968
For passaging, aggregates were centrifuged and resuspended in 500 µl of fresh medium, then a single-cell suspension was obtained by pipetting the pellet with a 1 ml syringe with a 26G needle (BD Franklin Lakes).
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PMC9596868
Data represents the mean ± SEM of three or more independent experiments.
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PMC4277423
In summary, we tested CCR5 gene disruption in HIV-1 susceptible cells using lentiviral vectors expressing Cas9 and sgRNAs CR1, CR2 or CR3.
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PMC8193046
The reference ABCB1 inhibitor 2 as well as the reference ABCG2 inhibitor 27 were purchased from Tocris Bioscience (Bristol, UK).
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PMC11612508
In this process, 1 g of natural flake graphite (45 µm, sourced from Asbury Graphite) was combined with 27 mL of a chilled H2SO4:HNO3 in a 2:1 ratio.
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PMC11422497
Cell counting kit-8 (CCK8) assay and (C) Annexin V-FITC/PI assay was performed to analyze the effect of circ_SMA4 on GISTs cell proliferation and apoptosis.
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PMC11237030
AQP5 expression on non-membrane and membrane fraction from CHO-K1 and CHO-K1/AQP5 cells (clone 16) assessed by western blot.
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PMC11653533
Distribution of Ficus species throughout the world.
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PMC10968586
Acute promyelocytic leukemia is a form of acute leukemia characterized by a chromosomal rearrangement involving PML::RARA fusion , making this malignancy sensitive to differentiating agents inducing PML-RARA fusion protein degradation by targeting the RARA or the PML part (all-trans-retinoic acid and arsenic-trioxide, respectively) .
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PMC8922418
The current PE system mainly includes PE1, PE2, PE3, and PE3b (Hsu et al., 2021).
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PMC11322866
Targeting EZH2 has exhibited therapeutic effects in various haematological malignancies .
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PMC11591794
The Hoechst staining solution was prepared by diluting the Hoechst stock solution 1:2000 in PBS.
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PMC9739791
To test whether the prodrug 30 could release oxaliplatin after two-photon absorption, the solution of the Pt(IV) prodrug 30 was irradiated with 100 fs pulses (725 nm, 800 mW/cm, 1 h).
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PMC11650848
We first designed a SNP pyroassay to assess polymorphism frequency at rs365052 in our cohort (Fig. 2, SNP Seq1).
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PMC11252553
on the frequency of apoptosis induced by various doses of X-rays after 48 & 72 hours post-irradiation.
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PMC9275501
Jiang et al. (2016) found that the antitumor activity of CUR combined with etoposide (ETP) was higher than that of CUR and ETP alone, and the dosage was reduced.
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PMC11774112
All tumors that had been excised from the mice are shown, corresponding to the groups designated above in Panel D. (F) Results of H&E staining of different organs and tumors in nude mice.
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PMC11045125
The expression of LMP1 in ΔEBNA2 EBV-infected CB B cells grown on the CD40L/IL21 expressing feeder layer is consistent with the known ability of IL21 to induce LMP1 expression in the absence of EBNA2 .
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PMC11769516
It has been shown that dietary supplementation with oils can reduce the degree of tissue damage caused by mycotoxin exposure.
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PMC9143157
These findings suggest the necessity of the further elaboration of nanomedicines for clinical application.
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PMC11422497
Additionally, the analysis of the relationship between clinical factors and circRNAs needs to be supported by larger samples.
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