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OTAR3088
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BioData-CellLine_annotated

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PMC11750712
Often forgotten, the TCR β-chain constant region is encoded by two genes: TRBC1 and TRBC2.
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PMC9878278
No: 9167L), STAT2 (Cell Signaling Cat.
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PMC11706776
A KCNN1 expression in A-673 cell line stably transduced with doxycycline-inducible shRNA control cells (shRNA CT) or 3 shRNA cells targeting KCNN1 (shRNA 511, shRNA 908 and shRNA 576) after 72 h of treatment with doxycycline (0.5 µg/mL).
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PMC10936243
However, these possible limitations or barriers from the UK, US and Canadian veterinary practices may or may not be present in other countries.
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PMC10748106
The bound colorant was proportional to either total protein or cells amount.
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PMC11322866
Using Fiji ImageJ 2.1.0 software, we split the color via the split channel and analyzed the images in 8-bit grayscale on the blue channel.
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PMC11718031
Heat map shows concentrations of amino acids quantified in the basal media The viability of shake flask batch culture cultivated in the top-performing media is shown in Fig. 2A.
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PMC11637276
First derivative of the curve for hit threshold for replicate 1. (
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PMC11594641
Nevertheless, contrary to our studies, the shRNA-mediated RAB27A knockdown in SkMel28 cells resulted in a 50% decrease in the sEVs’ total protein content .
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PMC11786704
GSEA analysis (Fig. 5C) revealed the enrichment of the MAPK gene set in the diabetic mouse model.
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PMC11574271
B The protein expression of RON in 5637 cells transfected with microRNA mimics or negative controls was assessed via western blotting.
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PMC10454535
The resulting accumulated protein within the cells results in endoplasmic reticulum (ER) stress .
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PMC11650848
Here we show that methylation at an alternative upstream promoter of ZFP57 is controlled in part by a quantitative trait locus (QTL).
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PMC11791264
H, overexpression of SETD7 (SETD7 OE) promoted the proliferation of SKOV3 cells determined by the CCK-8 assay.
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PMC9985266
TGF-β1 treatment significantly elevated p-ERK/ERK and p-p38/p38 protein levels in MRC-5 cells, and OE-MEG3 reversed this effect (Fig. 4B).
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PMC11317131
HEK293 (ATCC CRL-1573) and HEK293T (Thermo Fisher Scientific NC0260915) cells were maintained in DMEM + GlutaMAX (Gibco), HeLa (Sigma 93021013) cells were maintained in EMEM with l-glutamine (ATCC), K562 (Sigma 89121407d) human lymphoblast cells were maintained in RPMI-1640 media (Sigma), and U2OS (ATCC 30-2007) human osteosarcoma cells were maintained in McCoy's 5A media (Gibco).
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PMC6222726
Male flowers are most often 5-merous (sometimes 3- and 4-).
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PMC11763111
Each group has two replicates from different donors.
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PMC11528603
The main steps for preparing the extracts used in this study were presented in Supplemental Figure 1.
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PMC9436459
This demonstrated that a H-bonded ortho OH substituent on the chalcone was critical for the success of the [4 + 2]-cycloaddition.
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PMC11049294
A 40-cycle PCR program was used following a 2 min 95 °C activation, including 94 °C, 30 s followed by 55 °C, 30 s, and 72 °C, 40 s steps.
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PMC11240448
Strikingly, both the basal DPDL-B levels (Figure 1B and Supplementary Table S3) and the core network circuitry of MCF10A cells (Figure 2B and the SI file cSTAR_networks.zip) differ dramatically from luminal BC cells, suggesting that following oncogenic transformation these cells will become basal BC type rather than luminal BC type.
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PMC11594074
After 24 h, wound healing was quantified in MDA-MB-468 cells as 43.1%, 40%, 32.1%, and 28.2% and in HCT116 cells as 99.8%, 72.2%, 49.5%, and 3.1% after treatment with DMSO at doses of 0.5 × IC50, 1 × IC50, and 2 × IC50 of compound 4, respectively.
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PMC10178190
After 48 h, the medium was replaced with DMEM 5 mg/mL insulin.
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PMC11672142
In this experiment, 20 μM Fe(DefNEtTrp)2 (solution made by dissolving the isolated solid) was reacted with varying concentrations of Fe2(SO4)3 in separate solutions, up to 200 μM Fe(III) at pH 7.4 (50:50 (v/v) DMSO:H2O).
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PMC11767725
By harnessing the skin’s ability to generate both localized and systemic immune responses, it may be possible to design innovative treatments that leverage the skin’s natural immunological processes.
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PMC9429973
Image: Summary/Conclusion: In this research we describe a clinical case about the presence of CALR mutation in a JAK2-negative PV patient.
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PMC5839394
Protein identification was performed by MALDI TOF-MS/MS analysis.
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PMC11407042
Clustering of cells during passaging (Preparation one- culture and maintenance of A549 cells and Step-by-step- seeding A549 cells into 96-well plate).
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PMC9429973
Preclinical data have demonstrated increased activity with the addition of IMGN632 to AZA alone and to AZA+VEN in multiple AML xenograft and PDX models, leading to improved survival in these models (Kuruvilla.
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PMC11470999
We also employed a migration assay to compare the effects of PF and TMZ (Figure S1F), demonstrating that PF exhibited superior inhibitory activity on melanoma migration compared to TMZ in both B16 and A375 cells.
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PMC9398137
We identified BCL2 and MCL1 expression and occupational status of MCL1 as key factors determining resistance/sensitivity to S63845.
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PMC11528603
In addition, the unchanged cell population might be originated from the hindered proliferation and/or the balance between induced cell death and cell replication.
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PMC9429973
Image: Summary/Conclusion: A combination of techniques has classified a representative cohort of B-other-ALL patients from UKALL2003 into clinically relevant subgroups.
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PMC3730428
Although cIAP1 mRNA increased in response to AT-IAP (Figure 1d and Supplementary Figure 2B), its protein expression remained suppressed (Figure 1c and Supplementary Figure 2A).
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PMC5363531
In contrast, the combined treatment in MSTO cells resulted in a reduced apoptotic rate (Figure 2B).
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PMC8409415
In an investigation of CD70, NFkB‐p65, and AP‐1 expression after cisplatin exposure, rabbit monoclonal anti‐NFkB‐p65 antibody (1:1000, #3034, Cell Signaling Technology), rabbit monoclonal anti‐phosphorylation NFkB‐p65 (1:1000, #3033, Cell Signaling Technology) and rabbit monoclonal anti‐c‐Jun antibody (1:1000, #9165, Cell Signaling Technology) were used.
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PMC11592837
At this point, we noted that DOX-treated Jurkat cells and ETP-treated PBMCs exhibited peak levels of 53BP1 foci approximately 0.5–1 h earlier compared to γH2AX specific signals.
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PMC9429973
As surrogate marker of quality of life, we evaluated the prevalence of GVHD and immunosuppressive treatment (IST) in disease free patients.
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PMC10667689
Glucose consumption (PARS AZMUN, Iran) and lactate release (Audit, Iran) were established by subtracting the concentration of each metabolite in fresh media (C-RPMI with 10% and 15% FBS for HL-60 and THP-1 cell lines, respectively) from the final concentration in the cell culture supernatant of the control (Ctrl), PMA, and Gal-9 groups (Hitachi 917; Boehringer Mannheim, Germany).
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PMC11803918
In this study, taking advantage of both PDT and γδ-T exosome-based therapy, we engineered γδ-T exosomes to synergize with Chlorine e6 (Ce6)-mediated PDT for better performance against melanoma.
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PMC11531744
To evaluate the specific role of GBP1P1 in ovarian cancer, we examined the effects of GBP1P1 knockdown on the proliferation, apoptosis, migration, and invasion of W038 cells in vitro.
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PMC11618052
C NMR (100 MHz, δ ppm CDCl3): 161.35, 155.75, 147.26, 144.87, 135.41, 134.63, 130.26, 129.94, 127.32, 126.25, 126.04, 125.75, 121.70, 121.07, 119.47, 118.59, 46.39, 26.72.
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PMC11760643
PBMCs were recovered from the gradient interface, washed twice with PBS, and resuspended at a final concentration of 1 × 10 cells/mL for P2X7 expression assays or 3 × 10 cells/mL for CDC assays.
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PMC10253973
Ever since compound 1 was subjected to clinical trials in 1979, more work has been put into the development of metallopharmaceuticals.
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PMC8984067
Polyethyleneimine (PEI) has a large number of amino groups with high reactivity and polarity and an abundance of positive charges.
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PMC11730311
All hαCGRP8-37 analogues had detectable plasma levels 24 h post IV-injection.
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PMC11537649
Relative RNA levels were quantified using 2 method.
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PMC11786767
Supplemental Fig. S1 contains the results of Neuro-2A staining.
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PMC8494202
However, we must emphasize that public databases also enable access to sufficient numbers of research samples in a short period of time and encompass a variety of ethnicities, which would be difficult to establish within a clinical study.
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PMC9429973
For those who intend to continue treatment, romiplostim administration was extended to Week 52.
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PMC8633974
TatE gRNA reduced RT activity by an average of 76% (± 6.5%, standard error of the mean (SEM)), with more than 75% reduction in 6 of 7 tested HIV-1 strains.
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PMC10993311
Length (cm) was measured with a Seca 416 infantometer (Seca, Hamburg, Germany).
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PMC11747949
TruStainFcX (#422302, Biolegend) was used for nonspecific receptor blockade.
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PMC11711063
To determine ZG16B expression in vivo, MDA‐MB‐231 cells were implanted and grown as xenografts in SCID mice.
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PMC9429973
Methods: From July to November 2021, front-line clinical nurses in the hematology general ward of a tertiary hospital in Chongqing were selected as the research objects by convenience sampling method.
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PMC11473843
Mutated KRAS serves as the oncogenic driver in 30% of non-small cell lung cancers (NSCLCs) and is associated with metastatic and therapy-resistant tumors.
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PMC8873393
The cell pellets were resuspended in cell lysis buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.15% NP-40) and incubated on ice for 5 min.
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PMC11782508
SDS-PAGE of azurin fractions.
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PMC10158546
Signal intensity was quantified using imageJ and normalized to the signal from the strongest band identified in total protein images. (
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PMC3765139
Mice were euthanized after 12 weeks or in case of fulminate tumor growth.
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PMC11725127
GA 1 μmol/L, isoalantolactone, lenalidomide, YL064, adenanthin, oridonin, bavachin, and isobavachalcone at 10 μmol/L, respectively. (
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PMC11607638
In future studies, more attention should be paid to differentiate these two possibilities.
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PMC10813895
However, these and other hallmark aging changes in mammary epithelia occurs in women with pathogenic germline variants of BRCA1, BRCA2, or PALB2 even in the young to middle-age range who have pre-menopause status .
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PMC9693627
Then 0.5 mCi/mL [S] methionine was added.
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PMC11750696
The intricate relationship between ER stress and T cell functions is a burgeoning area of research with significant implications for immunology.
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PMC11224020
To provide further evidence of nuclear envelope tension being needed for cPLA2 activation in DCs, we used DCs lacking lamin A/C (Lmna-knockout (LmnA/C) cells), which cannot build up nuclear envelope tension in response to deformation.
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PMC10482220
To understand how G3BP is recruited to SGs, we fused each of its domains to GFP and expressed them in Cos cells to analyze their localization after treatment with arsenite.
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PMC11675244
Blocking the extracellular phase of Wnt signaling may be especially useful in cancers like triple-negative breast cancer or NSCLC, which are thought to harbor autocrine-like Wnt signaling .
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PMC11476004
A mutation of this gene entails the loss of its function, the formation of chromosomal instabilities, and the risk of cancer in dogs and humans .
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PMC11726848
The cells were analyzed within an hour using a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA, USA).
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PMC8956657
The IMPα3 is shown in dark gray and surface mode, and the p50 is shown in orange in stick mode.
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PMC11082662
We present this article in accordance with the TRIPOD and MDAR reporting checklists (available at https://tcr.amegroups.com/article/view/10.21037/tcr-24-309/rc).
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PMC10605143
DTHIB was added at the indicated concentration 48 h before heat shock.
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PMC6337547
The results of the liver function indicators serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed that salvianolic acid A (15 or 25 mg·kg) significantly reduced ConA-induced ALT and AST activity.
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PMC11787651
To meet the need for computational tools that enable large-scale identification and quantification in automated form with this new high-throughput method, we developed a generic data conversion software, EpiConverter, to format the “mzXML” file into “ms1” and “ms2” files, which can be analyzed with our EpiProfile software.
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PMC9429973
We detect cell cycle by flow cytometry after cells were stained with 50 µg/ml PI and then analyzed with ModFit LT 5.0.
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PMC9429973
SVR35, anemia, and bone marrow fibrosis improved over time.
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PMC11574271
At protein levels, cell line 5637 showed the highest RON expression.
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PMC5035325
4T1-model showed a weak to strong reactivity of EGFR and NeuGcGM3 (Table 2).
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PMC11469524
The corresponding network starting from the BL-2 model (without upstream wiring due to missing perturbations above PI3K and Btk) is presented in Fig 5C before and after training on HBL-1 and OCI-LY3 cell lines.
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PMC11473750
Instead, loss of transmembrane domain Helix 5 had the greatest effect on clonogenicity.
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PMC10530622
Slc26a6-/- mice have an increased net absorption of dietary oxalate, leading to hyperoxaluria and calcium oxalate lithiasis, highlighting the role of constitutive SLC26A6 in limiting net intestinal absorption of oxalate .
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PMC9429973
On the other hand, stem cells harvested from PB+BM increased from 0.3% in 2009 to 2.9% in 2018, while the use of PB stem cells remained stable during this time frame, spanning from 92% to 98%.
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PMC9429973
SCN patients have an increased risk of the development of myelodysplastic syndrome or acute myelogenous leukaemia.
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PMC11332076
Statistical analyses were performed using Prism 9 software (GraphPad Software, Boston, MA, USA).
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PMC9817179
All the tested concentrations of PFA except for 0% PFA (PBS only) result in a significant change of the LLPS parameters.
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PMC5600151
Besides, Kabanov et al. demonstrated that the DNA binding affinity with PPI dendrimers are generation dependent .
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PMC3681794
A, Western blots showing the time course of HIF-1α and HIF-2α stabilisation and protein levels of the HIF-1 transcriptional target carbonic anhydrase IX (CA IX) in 791T, HOS and U2OS cells after exposure to hypoxia.
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PMC10957991
Normally, mammary epithelium is bilayered and formed by inner luminal epithelial- and outer, contractile myoepithelial cell layer (B, lower picture).
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PMC9096373
At 1 day after administration, the mice were fasted to avoid asphyxia caused by gastric reflux during PET/CT.
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PMC9429973
Image: Summary/Conclusion: Mitapivat improved Hb and reduced transfusion burden in pts with PK deficiency over time, demonstrating the consistency and long-term durability of treatment with mitapivat.
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PMC10978090
7 It could be explained by the presence of heparin-dependent platelet-activating antibodies different from anti-PF4/H IgG, as the patient had alloantibodies.
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PMC11742290
Drugs targeting and inhibiting LGR5 can suppress tumor growth by regulating LGR5/WNT/β-catenin signaling pathway.
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PMC11697065
Cells expressing mAb1 reached peak viable cell density (VCD) on day 7, compared to other cell pools that reached peak VCD on day 9.
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PMC11607764
TP53 mutation and p53 are associated with clinical impact in LGG, and p53 pathway is disrupted in ∼80% of grade III and IV gliomas.
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PMC10033453
Injections were repeated at days 5 and 10.
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PMC11637276
However, the ECM may simultaneously pose a physical constraint for cancer cell invasion and tumour initiation .
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PMC11658074
h, i Intracellular PKH26 and CD151 fluorescence statistics. **
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PMC9424261
Tests to examine the differences between groups included Student’s t test and one-way ANOVA.
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