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OTAR3088
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BioData-CellLine_annotated

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PMC11711871
Male Sprague Dawley rats harboring the GR mutation (GR, EMMA stock number: EM:15187, ) were used throughout the study and generated by mating of heterozygous mutant male Sprague Dawley rats.
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PMC11723947
Source data are provided in the Source data file.
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PMC4485786
Nuclei are shown in green.
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PMC11635087
The coordinates of the position of CHO-K1 cells with chloroplasts were recorded, and cells in the same position were embedded in resin and sectioned, then observed using a scanning electron microscope (Fig. 5B).
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PMC9429973
Methods: 83 children patients were enrolled in this study and non-randomly assigned to receive 100mg/200mg (body weight 30kg) rituximab weekly for 4 weeks (group A, 53 cases) or a single dose of 375mg / m2 rituximab (group B, 30cases).
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PMC11457557
Overexpression of miR-21 has been shown to play a key role in the pathogenesis of DR by contributing to DM induced endothelial dysfunction as well as low-grade inflammation .
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PMC5941560
Insertion loci were only merged if they overlapped, and no hierarchy of tools was used for merging.
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PMC10154097
Knocking down sh-circSOX4 decreased cell viability (P < 0.01) (Figure 5(b)).
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PMC10329237
We measured the cytotoxicity of five serial dilutions of PFD (0.025, 0.05, 0.1, 0.2, and 0.5 mM) and LOS (0.05, 0.1, 0.2, 0.5, and 1 mM) to determine the non-toxic concentrations.
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PMC9275501
In vitro cytotoxicity experiments showed that HA-modified NLCs were better than BCL/DOX-NLCs in reducing the activity of breast cancer (MCF-7/ADR) cells, which may be due to the fact that HA ligands on the surface of particles can target HA receptors on the surface of tumor cells, thereby promoting the entry of BCL and DOX into cancer cells.
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PMC11496728
The CRISPR/Cas9 system expression vector PX459 was linearized using the restriction enzyme BbsI, and the sgRNA and linearized Cas9 vector (PX459-Apaf1) were incubated at 16 °C using T4 ligase and ligated overnight.
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PMC11779960
The challenge with this analysis is determining whether the TCRβ rearranged cells falling outside expression of the dominant Vβ are malignant or derived from contaminating normal T cells, which may also be clonally expanding.
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PMC11420784
n = 3 slides.
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PMC10203589
In conclusion, FGF18 affects the development of GC cells at different levels, and can be used as a novel prognostic marker and therapeutic target for GC.
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PMC11802929
For each cell captured, antigen-specificity was identified through the dominant pool hashtag (Supplementary Fig. 1a) and dextramer (Supplementary Fig. 1b).
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PMC11194678
Numerous studies have shown that STAU1 directly interacts with the 5′UTR of target mRNAs to enhance their translation (Dugré-Brisson et al., 2005; Ricci et al., 2014), so we generated the 5′UTR of MTOR mRNA by in vitro transcription, and electrophoretic mobility shift assay (EMSA) results showed that STAU1 WT and the LLPS-deficient 5KE mutant bound to MTOR 5′UTR mRNA with similar affinities (Fig. S2 C).
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PMC11279397
In the field of traditional Chinese medicine, thymol has been utilized for an extended period as an expectorant , anti-inflammatory , antiviral , and antibacterial agent specifically targeting upper respiratory system diseases.
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PMC11680982
Of these, the ZR-75-1 cell line had pronounced HER2 expression compared with other breast cancer cell lines (Fig. 1A, B).
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PMC11730320
The reconstructed germlines were used in downstream analysis to infer somatic mutations and reconstruct lineages.
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PMC8348645
This might be further studied for the identification and isolation of the bioactive component responsible for its antioxidant activity.
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PMC11765988
Pulmonary Fibrosis: Antifibrotic treatment (nintedanib) may be added to the therapeutic regimen, and in severe cases, lung transplantation might be necessary .
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PMC7553912
The total area and positive area by Pizzolato staining in each kidney section were measured using Image J (US National Institutes of Health, Bethesda, MD).
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PMC11628904
These observations suggest that, at least in zebrafish, C. albicans colonisation of burned tissue relies on viable keratinocytes physically migrating into zone 1 tissue where C. albicans initially exists.
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PMC3765139
A number of other genes upregulated in CD133+ D10 cells encode proteins involved in cell proliferation, including insulin-like growth factor-1 (IGF-1) and its binding protein insulin-like growth factor-binding protein-3 (IGFBP-3).
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PMC10553685
Disclosure: C. Ufondu: None.
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PMC11745823
Utilising the JASPAR database , it was observed that HIF‐1α binds to the RIT1 gene promoter.
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PMC11321678
Therefore, we hypothesized that NTRs are tightly associated with MBs, and that different molecular subgroups may have different types of NTRs.
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PMC11506827
Our study emphasizes the importance of investigating the effect of natural compounds such as GEF in cancer research.
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PMC11471157
To address this issue, our research pivoted towards utilizing dried fish, a rich source of proteins including collagen.
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PMC11721277
However, as the incubation time was prolonged to 30 and 60 min, the RCY experienced a decline to 74.5 ± 0.4% and 69.7 ± 0.5%, respectively.
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PMC11075223
Processing is artificially altering the chemical composition based on their intended use and is a recreation of the quality of medicinal materials.
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PMC7338939
Output of the mathematical model (n(t)) and the measured tumor size, CK(u(t)), and R(u(t)) for four different animal models.
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PMC10957991
GAPDH was used as a loading control. (
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PMC8143558
The results from the study showed that the saponins inhibited the growth of all three Gram-negative bacteria, with P. aeruginosa being the most susceptible .
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PMC11088135
B Dual luciferase reporter assay was performed to confirm the interaction between lncRNA NUTM2A-AS1 and miR-376a-3p. **
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PMC9684669
Using all 177 genes involved in the calcium pathway, unsupervised hierarchical clustering revealed a clear separation of these two cell types ( Figure 4E ), demonstrating a major modification in the calcium homeostasis of MDR cells.
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PMC6160470
Procedures and methods for crown ether synthesis are shown in the Supplementary Information.
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PMC11541409
The most common PDGFRA mutations occur in the TKD C lobe (exon 18), resulting in enhanced ATP affinity and/or catalytic velocity (Fig. 1B).
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PMC9429973
This pipeline was used to stratify patients via a dimension reduction approach whereby clustering was performed on 210 gene signatures categorised by 10 different hallmarks of cancer.
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PMC10092581
The GR activity was estimated on 50 μg of the protein in 0.2 M Tris‐HCl buffer (pH 8.1), 1 mM EDTA, and 0.25 mM NADPH.
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PMC4270159
Serum starve HL60 cells for 24 hr prior to protein harvesting.
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PMC11592837
A total of 15 µL reaction volume was employed in all qPCR analysis, containing 1× PowerUp™ SYBR™ Green Master Mix and 0.25 µM of primer.
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PMC11661452
For clarification, the following text has been added to the fig. S2 caption: “TEM images of α-EGFR/α-CD16/α-4-1BB NPs and α-EGFR/α-CD16/α-4-1BB EPI NPs are the same as Fig. 1B.” •
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PMC10243817
In this study, we found a downregulation of collagen production in nasal epithelial cells in eosinophilic CRSwNP characterized by predominant type 2 response, suggesting distinct regulation mechanisms for collagen production in different cell types and disease contexts.
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PMC11794847
Through flow cytometry analysis, the distribution of fluorescent signals from each cell was recorded, allowing for the quantification of CD69 expression levels across the cell population.
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PMC11694066
Cells were then continuously cultured in the presence of small-molecule inhibitors for a period of 5 days, at which point, cell viability was estimated by adding 20 μL of CellTiter-Glo (Promega), diluted 1:4 in PBS to the medium.
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PMC9847912
For these HIV-2 and SIV isolates, the level of infection at the highest concentration of GSK232 used in the assay (40 nM) was >50% of that seen in no-drug control wells from the same assay plates.
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PMC11350568
As delineated in Figures 5e,f, and S5b,c, the noticeable reduction in the binding trend of Cy5-Sgc8c in the AptG-1-treated group, subsequent to preincubation with cells, unequivocally affirms the system’s adeptness in efficiently degrading PTK7 within the cellular membranes of A549 and MV-4–11 cell lines.
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PMC10631132
Scale bars: A, C, E, G = 100 µm, B, D, F, H = 25 µm.
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PMC7057940
In addition, translocation t(11;22)(q22;q12), characteristic of ES, was observed at a low frequency (2%; Fig. 2A), as well as translocation t(1;16)(q21;p13), also reported for this type of tumor (Table III).
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PMC11615218
Nevertheless, there is an overlap between T-cell senescence and exhaustion; for example, the novel exhaustion marker TIGIT was also found to be upregulated in classic senescent CD8 T cells .
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PMC9884169
In order to further understand the effect of gene knockdown on autophagy inhibition, we applied the autophagy inhibitor CQ (Selleck) to amplify the inhibitory effect of autophagy and detect the effect of MCT4 on the degree of apoptosis by Annexin-V-FITC Apoptosis Kit.
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PMC11437637
The inclusion criteria for the uterine fibroid group were as follows: 1) no malignant lesions in the endometrium; and 2) having complete clinical and pathological data.
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PMC9429973
8: NSMCE2).
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PMC11637276
c, f, i) Relapse-free survival of patients with PDAC with high (red) or low (black) α2 integrin (ITGA2, c), β1 integrin (ITGB1, f), and MAP3K1 (i) expression.
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PMC7812570
To measure the inhibitory activity of GPI-m36.4 or GPI-FluIgG03 in transduced TZM-bl cells, 200 TCID50 of virus was added to the cells (1 × 10/well) and incubated at 37 °C for 48 h. The cells were lysed and quantitated for luciferase expression as described above.
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PMC11525028
Data was considered as not statistically significant (ns) when p > 0.05.
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PMC8992508
The compounds that showed the best inhibitory activity toward BCRP were characterized by the presence of hydroxy, cyano, nitro, acetamido, and fluoro groups at the 4-aniline scaffold.
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PMC4112127
The promoter region of BMP3 was found to be methylated in all five biliary cancer cell lines tested, but not in an immortalized benign cholangiocyte cell line, and methylation of BMP3 was associated with either low or absent BMP3 gene expression in these biliary cancer cell lines.
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PMC9429973
The median (range) age was 37.5 (18-69) yrs, 75% were male, and all (100%) patients were Asian.
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PMC9895440
While none of the highest-ranking genes of our CRISPR-Cas9 screen were statistically significant due to experimental variation, the screen allowed us to prioritize genes for single gene validation experiments and thereby discover SNX9’s role in T cell exhaustion.
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PMC11806182
F Heatmap depicting the relationship between four co-expression modules and miR-424.
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PMC10999934
We jointly analyzed the transcriptomic profiles of single-cell and bulk datasets of HGSOC to identify cell types associated with chemoresistance.
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PMC11545737
UreB has been found to play a role in pyroptosis.
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PMC7519871
B-cell lymphoma 2 protein (Bcl-2) PDB ID: 4LXD; center x = 22.02, center y = 29.36, center z = 6.19 size x = 28, size y = 22, size z = 22.
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PMC3274243
We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro.
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PMC11711935
These senescent fibroblasts stimulate pigmentation by upregulating the expression of melanogenesis regulators, such as MITF and tyrosinase, in melanocytes .
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PMC9429973
Patients with myeloid predominant Decitabine response harboured an increased number of mutations in signalling and kinase proteins encoding genes.
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PMC4277423
Fig. 5A shows that 42.5% CCR5 negative cells was obtained in CEMss-CCR5 cells co-transduced with vectors expressing Cas9 and CR2, but no CCR5 reduction in cells co-transduced with vectors expressing Cas9 and GF1 control.
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PMC9429973
After induction of PML-RARA expression with Zinc for 0-24h, BCL2 protein levels were evaluated by western blot.
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PMC11753435
Providing only the target sequence of UltraID and confirming their catalytic pocket targeting via AlphaFold-Multimer, we generated 20 candidate IPs (IP_PpC_1 to IP_PpC_20) with PepPrCLIP (table S1 and data file S1).
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PMC8996378
The structures of diterpenes have important guiding significance for further searching for new drugs to reverse tumor MDR.
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PMC8956657
A summary cartoon is presented, highlighting the minor and major site interactions and the structure presented in the right panel.
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PMC9429973
In recent decades, the treatment of PCNSL has undergone significant changes; its standard treatment consists of combined immunochemotherapy regimens that should include high-dose methotrexate (HD-MTX) and/or cytarabine, followed by consolidative autologous hematopoietic cell transplantation (AHCT).
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PMC11564322
The mRNA expression was normalized to the GAPDH gene.
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PMC8875242
Velpatasvir inhibited ABCB1 only in hPCIS.
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PMC11787381
Through IN route, sub-chronic administration of low doses (300 μg/kg) of formulated bambuterol prevents long-term spatial memory impairment and demonstrates favorable effects on the survival of hippocampal neurons and preservation of synapses.
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PMC11612794
Solvent A and B compositions were 100% H2O, 0.1% Formic acid and 100% Acetonitrile, 0.1% Formic acid, respectively.
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PMC11361748
These inhibitors together with 1 μg/ml of TNFα neutralizing antibody Infliximab (Novus Biologicals #NBP2-52655) do not alter neutrophil migration in response to fMLF, a potent neutrophil chemoattractant , suggesting that neutrophils can respond normally to other chemotactic cues in the presence of the inhibitors (S3H Fig).
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PMC11089031
But we found that in TCGA ccRCC cohort TM9SF2 was associated with good prognostic.
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PMC11578343
Cells are intrinsically dynamic viscoelastic materials, consisting of elastic biopolymers and viscous fluids that comprise the cytoskeleton and the cytoplasm, respectively. , ,
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PMC11746948
The protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific, USA).
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PMC7757104
Although studies have reported an association between tOPN expression and chemoresistance (10,12–15), the functional roles of specific OPN-SIs in chemoresistant cells have not been previously determined.
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PMC11802929
7Longitudinal analysis for PG-TCR groups.a Tracking SARS-CoV-2 CD8 T cells from acute to post-acute timepoint based on TCR-GLIPH query from a previously reported longitudinal dataset.
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PMC10955424
Malignant ascites (n = 13), cirrhosis ascites (n = 2). (
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PMC11394227
When these two reactions are combined, tGSH (GSSG + GSH) acts as a rate-limiting factor for color production, and the amount of tGSH determines the amount of yellow TNB formed.
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PMC11632064
Since proliferative and inflammatory statuses are tightly associated with phenotype switching in melanoma (reviewed in Hossain and Eccles ), we examined the impact of BI-D1870 on the expression of transcripts previously associated with seven phenotypic states (from differentiated/melanocytic to undifferentiated) (Tsoi et al, 2018).
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PMC11695623
Knocking down CDK1 can inhibit the proliferation of LUAD.
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PMC7802142
a Schematic illustration of ReN-NV preparation.
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PMC9398137
Molecular mechanisms leading to aberrant MCL1 expression have been studied in DLBCL.
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PMC9429973
Then the conditioned medium (1L) was used to isolate the EVS with Tangential Flow Filtration (MWCO 500KDa).
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PMC7022782
At 80–90% of cell confluence, trypsinization was performed and the cell layer was harvested with CM.
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PMC11610461
D, E) The results of qPCR assays for five groups of cancer and paracancerous tissues (tumor: n = 5, normal: n = 5; G2: n = 2, G3: n = 3). “*”, “**”
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PMC11388384
Therefore, we speculate that the SCUBE1–SHH transition state complex is distinct from the relatively stable state in which SHH diffused freely.
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PMC11055323
These results suggest that SS18-SSX possess a unique ability to promote long-range chromatin interactions and a more compact architecture by at least enriching H2AK119ub modified chromatin.
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PMC9111184
Fig. 4Analysis of FTIR spectra of Control and m-CA, ChA, RA, EZ treated cells with PCA.
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PMC11518702
C1ORF150’s pY89EN site closely conforms to HGAL pY107EN (a confirmed GRB2 binding site) (3, 8, 37).
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PMC9429973
Chest x-ray and transthoracic echocardiograms were performed at baseline & 24 mos.
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PMC3675084
Together, the data indicate that caspase-2 and caspase-3 independent cell death was involved in both the parental and paclitaxel-resistant ovarian cancer cells; the predominant cell death pathway was through apoptosis in parental cells, and mitotic catastrophe in resistant ovarian cancer cells.
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PMC11578343
Both the low‐to‐high and the high‐to‐low frequency devices showed decreasing elastic G′ with respect to increasing frequency, just as in our previous experiments.
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