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OTAR3088
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BioData-CellLine_annotated

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PMC11727145
We retained the basal medium containing DMEM and Ham’s F12, which provided amino acids and vitamins, along with FBS, which is crucial for maintaining physiological balance and promoting cellular proliferation .
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PMC11740573
Similarly, calcitriol pre-treatment of endothelial cells decreased the lipopolysaccharide-induced IL-1β gene expression more than calcitriol post-treatment .
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PMC11078693
Incubate at 20°C–23°C on a rocking platform at 2 rpm for 30 min.h.
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PMC11180402
J-K Colony formation in 5637 (shPTEN) vs. 5637 (nonsense) cells in soft agar with or without ISO treatment was quantified.
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PMC11607321
MOSA augmented the proteomics data by filling approximately 32% of the original matrix using information from all omics, while preserving sample correlations with an independent proteomic dataset (CCLE) (Supplementary Fig. 5b, Supplementary Data 7).
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PMC10165258
That was also the case for the droplet library producing chips.
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PMC11448304
d KEGG functional classification on upregulated proteins.
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PMC11743316
The PI3K-Akt pathway is an internal signal transduction system that stimulates proliferation, metabolism, cell survival, and angiogenesis in association with external signals.
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PMC11519788
Therefore, SPOP may regulate cancer stemness through multiple dimensions in a cancer content-dependent manner.
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PMC11705630
As such, the modulation of enzymes involved in the nucleotide metabolism could alter deoxynucleotide triphosphate (dNTP) levels, thereby either suppressing or stimulating cell proliferation and replication.
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PMC11473750
Cell viability was measured using the Alamar Blue assay (Life Technologies) according to the manufacturer’s protocol.
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PMC11790971
This aligned with our t-SNE visualization results, suggesting that morphological features alone are insufficient for distinguishing all cell lines.
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PMC6102174
13Structures of compounds 185–198 Structures of compounds 185–198 This paper summarized the progress of anti-HBV constituents from medicinal plants in China.
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PMC11748335
Existing research also supports the notion that peaks located outside of promoters can regulate gene expression.
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PMC11721277
The vast majority of currently available diagnostic radiopharmaceuticals is used in positron emission tomography (PET) or single-photon emission computed tomography (SPECT) .
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PMC11781181
Cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Bio-Rad).
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PMC10944868
Computer based ADME studies, also known as computer-based studies, play a crucial role in the drug discovery and development process.
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PMC11201989
In summary, we have demonstrated that PD-L1 contributes to both primary cilia biogenesis and function.
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PMC10996034
In the process of enhanced cell migration, there is an inevitable generation of an EOC cell population in the EMT transition state (49), which leads to the development of Taxol resistance.
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PMC10808409
Dot plot representing four quadrant images recorded by flow cytometry analysis for cells stained by Annexin V-FITC and propidium iodide.
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PMC8992508
The P-gp and BCRP inhibition by these tariquidar derivatives was found to be associated with the inhibition of ATP hydrolysis.
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PMC7812570
Given that GPI-FluIgG03 also exhibited inhibitory activity, the expression of a membrane-anchoring scFv via GPI in lipid rafts might interfere with viral budding in a nonspecific manner, which was previously observed.
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PMC11206251
Next, the cell viability and morphological changes were evaluated by MTT assay and Hoechst staining.
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PMC5025404
1Clone selection scheme (adapted from User guide for Freedom™ DG44 Kit) and development of stable cell lines for protein production Clone selection scheme (adapted from User guide for Freedom™ DG44 Kit) and development of stable cell lines for protein production Limiting dilutions were used to select individual clones.
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PMC11489351
Thus, the results are confounded by off-target compensatory effects arising from NHE1 deletion, followed by clonal selection of the line.
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PMC11394730
Although there is significant evidence about the potential preventive and therapeutic effects against cancer, a significant consideration regarding its safety at high doses must be considered .
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PMC10158546
Our findings are striking because others have reported that similar cultures derived from differentiated neural progenitor cells can be infected with prions [17–24].
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PMC10938336
The formazan crystals were dissolved in 100 μL of DMSO (spectrophotometric grade) in the dark for 5 min, and the absorbance (OD) of each well at 550 nm was read with a microplate reader.
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PMC11803210
Venn diagram of genes expressed (average TPM (transcript per million) 10) in vehicle control- (DMSO) and 10 µM naringenin-treated MCF-7 cells.
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PMC11025866
The adjusted p-value was reduced to < 0.05 to reduce the false positive rate when investigating individual genes.
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PMC11772585
A tissue array (HSkiC060PT01) containing 36 cSCC specimens and 7 normal cutaneous specimens was obtained from Shanghai Outdo Biotech Co., Ltd. Tumors were classified according to the cSCC Broders Pathological Classification.
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PMC11564405
CD4 + T cell infiltration density was categorized into low-density (≤ 187 cells/mm) and high-density (> 187 cells/mm) groups.
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PMC10938336
In addition, network pharmacology and docking analysis indicated synergistic effects, targeting both the CASP3 and PARP1 proteins by monocarbonyl curcuminoid.
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PMC10040136
The following constructs were used: shTgfbr2 with HPGK-PURO-CMV-TagRFP (Cat No. TRCN0000294600, Sigma, USA).
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PMC3888431
Additionally, 47,235,395 reads were sequenced with Illumina’s Genome Analyzer IIx applying 2×150 bp paired end sequencing mode.
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PMC9429973
In the cox regression analysis, the HR of D-PCR BCR::ABL1 below the cut-off for MolR was 0.48 (95% CI 0.35-0.66, p<0.001).
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PMC11495567
After incubation, 400 μL of 1X annexin binding buffer was added to each tube, mixed gently on ice and immediately analyzed using the Sony SH800 Cell Sorter with Software version 2.1.6 (Sony Biotechnology, San Jose, CA, USA).
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PMC11627060
To our knowledge, this is the first study to investigate the signaling pathway by which TQ-BSA NPs induce apoptosis in A549 cells via a caspase-independent mechanism.
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PMC9429973
Results: The platelet count was lower in patient subgroups compared with CG (p<0.001), but did not differ between patient subgroups (p=1).
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PMC11703992
The intercellular communication analysis revealed communication characteristics of CD4+ Memory T cells in GCA and elderly samples.
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PMC9429973
Three out of the 12 patients only detected by MFC (25%) would change to a higher IPI score-risk.
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PMC9964654
The Au uptake by A2780 treated cells was ca.
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PMC11317131
On day 15, the media was harvested and vWF was detected using the vWF Human ELISA Kit (Invitrogen) according to manufacturer specifications.
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PMC11789597
Upon activation, autophagy can remove ROS from the cells and function as an effective strategy to maintain redox homeostasis.
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PMC9841531
3-BP, on the other hand, is a regular chemical without regulatory constraints, promoting general use; (b) radiotracer assays are accompanied—apart from regulatory constraints—by complex protocols, which makes the determination of full-blown concentration-effect curves or even high-throughput screenings a challenge.
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PMC11190538
The surface morphology of nanophytosomes containing C-PC confirmed the formation of spherical particles and homogeneous dispersion.
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PMC11782780
These findings unveiled that miR-301b-3p downregulated EBF3 to mediate the glycolysis pathway and promote BC cell stem-like phenotype.
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PMC10788478
Two weeks after the initial treatment, the colonies were stained with methylene blue and counted.
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PMC11594641
Furthermore, A375 cells release a higher amount of pro-invasive factors such as VEGF, MMP-2, or TNF-α (all necessary for the degradation of ECM) compared to less aggressive SkMel28 cells .
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PMC10957991
As TRPV4 depletion, in the mammary epithelial cells, led to alterations in the pathways upstream of actomyosin assembly and consequent lower cellular force production, this could be one reason for the loss of epithelial integrity (Figs. 4A,B, S6).
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PMC11569208
Supernatant of infected cells with different MOI were assessed for VSV∆51 viral titer 24 hpi by viral plaque assay (n = 3, mean ± SD; *P < 0.05, ***P < 0.001, ****P < 0.0001 by two-way ANOVA). (
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PMC11527437
A major limitation of TCR-T, however, is that a given TCR only can treat an often-small subset of patients due to the dual requirement of the specific tumor antigen and HLA expression.
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PMC11588085
Different cell types as labeled as well as the percentage of cells with gene expression >0. (
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PMC11082662
Analysis of secreted proteins in TCGA-OV via a volcano plot revealed genes with a significantly high expression gene (in red) and low expression (in green) based on the selected cutoff values (|log2FC| >1 and P<0.05), with SMPDL3B, PTGS1, and IL4I1 identified as target genes meeting specific criteria (log2FC >4.2 and −log10(P value) >47). (
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PMC11585587
To ensure consistency, the protein mass, volume, and concentration were adjusted to the same levels, and 50 µg of total proteins were separated which based on different molecular weights by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
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PMC11727638
In TK cells cultured in the high-density Cell Domes for 3 days, the cells stained with hypoxia probe solution also showed red fluorescence throughout the inside of the high-density Cell Domes, indicating hypoxia.
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PMC10936243
And, sometimes, why we don’t really put the bedding in all situations, because sometimes during the recovery process, they will pee and poo… we don’t want the stool or urine to dirty the body” [D122]. “
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PMC11095939
Then, 1 mg of total protein was incubated with anti-Mecp2 (5 μg) antibody overnight at 4°C with constant mixing.
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PMC11347429
Trimmed reads were mapped to human genome annotation GRCh38 (ENSMBL Homo_sapiens.
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PMC9473868
These results suggested that BAI significantly inhibited the lateral and vertical migration of SW982 cells via regulation of epithelial-mesenchymal transformation (EMT)-related proteins and MMP family proteins expression.
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PMC11779993
Importantly, we also elucidate the molecular mechanism behind wound-healing effects of SPEE on keratinocytes, which have not been previously reported.
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PMC10170482
For image processing and quantitative analysis, we used the ImageJ software (NIH, Bethesda, MD, USA).
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PMC11726183
In vitro, cytotoxicity assay for the synthetic derivatives was determined by performing an MTT (yellow tetrazolium salt, 3-(4,5-dimethylthizol-2-yl)-2,5-diphenyl tetrazolium bromide) assay by using an aggressive breast cancer cell line MDA-MB-231.
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PMC4355729
Compared with ACHN-vec cells, the mRNA expression of PIK3R1 and ECAD was increased in ACHN-PIK3R1 cells, whereas the expression of NCAD, VIM and ZEB1 was decreased (Supplementary Fig. 3a).
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PMC9105697
DNA was eluted in 80 µL of RNase-free water.
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PMC6182045
The concentration of either anion exchange- or immune-affinity chromatography purified TZ97008 rgp120 from MGAT1 CHO cell line was confirmed by BCA (Thermo Fisher, Waltham, MA).
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PMC11761919
More than 90% of E. faecalis/Fiber2, E. faecalis/Fiber2-insulin and E. faecalis/Fiber2-PINs were completely released from the microcapsules within 15 min, and 100% released within 1 h (Fig. 3B).Fig.
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PMC9621239
Phylogenetic trees were constructed using MEGA 7.0 for analysing the evolution of HBV X QS in the liver tissues.
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PMC10589262
Results of this study show evidence that GIST has developed an ATF6-dependent UPR activation mechanism to ameliorate ER stress.
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PMC11535726
There are three dendryphiopsis-like species (K.inthanonensis, K.nabanheensis and K.septemseptatum) that constitute a strongly-supported subclade, but they are nested within sporidesmium-like species.
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PMC11792740
Deterioration in the clinical course: T-cell depletion may lead to a worsening clinical trajectory, characterized by exacerbated symptoms, increased tumor burden, and reduced survival.
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PMC11098378
Fold change in GFP signals were calculated by dividing normalized GFP values of experimental treatments with normalized GFP values in control treatments (indicated in each figure).
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PMC9429973
It is challenging to identify such patients at diagnosis.
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PMC9429973
Co-expression of CCND1, CD79A, and VPREB3, and a larger predominant subset, were in the venetoclax-sensitive subgroup.
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PMC9429973
For each sample at least 200 interphase cells (iFISH) and 5 metaphases (mFISH) were analyzed to increase sensitivity.
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PMC11509224
Only one diterpene was generated by Luffariella sponges.
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PMC11354239
These CD20 mutations involving the RTX epitope are less present in DLBCL, but are still able to cause irreversible resistance to RTX .
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PMC11443022
Tumor development typically occurs around the age of 15, emphasizing the importance of early surveillance.
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PMC7351993
The real-time Raman image processor (hybrid FPGA-CPU infrastructure) is the brain of the RIACS that connects the event detector, PD array of the SRS microscope, digitizer array, multiple FPGAs, and two actuators of the dual-membrane push-pull cell sorter on the 10-Gbps all-IP network to digitally process 24-parallel image detection signals and 1 event detection signal (Supplementary Fig. 4, see “architecture of the real-time Raman image processor” and “signal processing in the real-time Raman image processor” in the Methods section for details).
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PMC7090062
GISTs occur at every level of the gastrointestinal tract, but most commonly in the stomach (60–70%), and small intestine (20–30%).
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PMC9429973
Currently, most of the knowledge comes from clinical trials (CT), with stringent inclusion criteria and poor representativeness.
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PMC7185206
To test the hypothesis that ADAM10 and ADAM17 cleave surface PD-L1, we compared normalized RNASeq FPKM for ADAM10 and ADAM17 in tumor samples with PD-L1 protein-to-mRNA ratios in papillary renal cell carcinoma by linear regression model (Figure 1(d)).
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PMC11323699
Additionally, the combinatorial treatment proposed in this previous study contrasts with our sequential treatment approach, which positions TAX2 in the post-PARPi setting, an area of significant unmet medical need.
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PMC11365983
Similar transcriptional changes in the meninges of Foxc1 and Pdgf pathway mutant mice. (
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PMC11590005
An oficial national survey also observed an increase in non-whites enrolled at universities .
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PMC10898159
To date, no studies have associated lncRNA treatment to altered expression of KIT gene and mutations in GIST, despite the fact that some lncRNAs that have been shown to regulate KIT expression in GIST and are associated with imatinib resistance .
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PMC9822769
Although dozens of papers are dealing with the mechanism of cellular accumulation of cisplatin and its homologues , very little is known about Pt(IV) prodrugs.
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PMC9429973
11% of patients achieved efficacy between 7 and 12 months.
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PMC11657744
The x-axis represents the DNA content, and the y-axis represents the number of cells.
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PMC11680562
The spreadability of the PXFCu gels was assessed by determining the expansion of 2 gm of gel in the middle of two horizontal glass plates (20 cm × 20 cm) for one minute.
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PMC6163708
In three normal control cell lines, the HH proteins were also present.
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PMC7039683
Raw and processed sequencing data for zebrafish, mouse and human ATAC-seq were deposited in GEO repository (GSE140241, GSE139945 and GSE139809).
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PMC11683240
To our knowledge, no data are available regarding the α1AT expression in humans during B. pertussis infection.
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PMC10788478
Abbreviation: LGR6, leucine‐rich repeat‐containing G protein‐coupled receptor 6.
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PMC9429973
As a control group, we included 10 nonHABMF patients with AA (n=6) and refractory cytopenia of childhood (n=4), all treated with IST with poor response in 6 patients, as well as 10 samples of healthy donor BM grafts and 8 newborn peripheral blood (NBPB) samples.
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PMC11746948
Cells were seeded at an appropriate density and cultured for 24 h. Subsequently, the cells were treated according to the descriptions in individual experiments.
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PMC11746942
This self-reinforcing circuit likely contributes to the persistent inflammation and keratinocyte hyperproliferation observed in psoriatic lesions (Fig. 6F).
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PMC7841067
After 4 weeks, mice were sacrificed, and the tumor was resected and weighed.
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PMC11098378
Only signal above the set threshold was highlighted and percent area of each field of view with fluorescent signal was measured using the Fiji analyze particles function.
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PMC9429973
After a median follow up of 27.1 months, the 36-month PFS and OS were 62.0% and 79.5%.
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