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BioData-CellLine_annotated

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PMC9429973
RNA-seq was performed with total RNA isolated from U937 cells treated with 2μM DZNeP for 48 hours.
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PMC10873328
Additionally, Matrigel (BD Biosciences) was performed for invasion assay.
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PMC11588085
Gene Ontology analysis of down-regulated genes in OVCAR3-KRAB with sgXIST7(Left) and sgXIST9 (Right). (
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PMC8345486
Necrotic cells (PI+/annexin V−) are generally dead, that is, devoid of vital functions, which may interfere with the assessment of these parameters for living cells (underestimate the results).
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PMC9910796
Reads obtained were mapped to the expected vEP84Ri reference genome with Bowtie2 aligner software using default parameters.
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PMC11587606
These experiments were repeated two times under identical conditions.
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PMC11335267
Immunofluorescence labeling confirmed co-expression of Alx3 in orexigenic NPY/AgRP neurons expressing Agrp-tdTomato (Fig. 6F) and in anorexigenic POMC/Cart neurons expressing Pomc-GFP (Fig. 6G).
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PMC11496491
Our exclusion criteria include: (1) The patient had no detailed medical history; (2) The patient did not cooperate with routine follow-up or medical treatment; (3) Diabetic foot ulcer combined with other serious diseases that affect treatment; (4) The patient failed to obtain wound edge specimens after TCTD.
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PMC11773391
The pcDNA3.1‐MGMT OE vector was produced by Sangon Biotech (Shanghai) Co. Ltd. The plasmid sequence was verified by DNA sequencing supplemental data.
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PMC11740207
Cells employing the ALT pathway typically exhibit telomeres of varying lengths—some significantly shorter and others considerably longer than typical telomeres (Sobinoff and Pickett, 2017).
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PMC11371627
We then investigated whether the KEL gene expression was diminished on RNA level.
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PMC11742290
It is difficult to produce a stable and effective LGR5-targeting cancer treatment as LGR5 is involved in both WNT and TGFβ pathways which has a dual role in modulating tumor progression.
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PMC11468365
The performance of MultiComb is evaluated using the O’Neil cancer benchmark dataset, which includes drug combinations and their effects on various cell lines.
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PMC11200978
Samples exposed to a single −15 °C freeze yielded 64.1% (±6.6) live, 30.6% (±8.4) necrotic, and 4.0% (±2.9) apoptotic subpopulations.
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PMC10999934
The differentially expressed genes (DEGs) between malignant epithelial cells associated with chemoresistance and those not associated with chemoresistance. (
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PMC11144382
In addition, 3 unrelated control human fibroblast cell lines (CRL2072, CRL2106, CRL1475) were obtained from ATCC (LGC Standards, Middlesex, UK).
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PMC9429973
At baseline 69 (67%) pts received imatinib (IM) and 34 (33%) pts-second-generation (2G)TKI (Tab 1).
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PMC11025866
While treatment with NaB+VPM includes an HDACi, the gene expression pattern in both replicates after the addition of VPM to NaB was different than in cells treated with NaB alone.
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PMC11791432
Different from the above literature , after the immune cells were grouped, the data showed that ANXA1 was up-regulated in T cells instead of M2 cells.
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PMC10747160
Another facet of Griffithsin’s use as a prophylactic agent is the theorized importance of Griffithsin’s ability to cross-link target proteins together.
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PMC11087055
Figure 3—figure supplement 1.Time evolution of cell flow in control-GFP/COS-implanted embryo.(A) Trace of cell flow path of electroplated epiblast cells by using Imaris tracking analysis.
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PMC11718274
This cascade of events suggests the induction of the ROS-mediated mitochondrial pathway of apoptosis.
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PMC8998437
In actively proliferating PTLD and LCLs generated by a viral infection of resting B cells in vitro, EBV expresses the full set of latency type III (latency III) proteins: six nuclear antigens (EBNA1, EBNA2, EBNA3A, EBNA3B, EBNA3C, and EBNA-LP) and latent membrane proteins (LMP1, LMP2A, and LMP2B) (reviewed in ).
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PMC11774747
Most of the CAR-VST reported in the literature are generated from VST obtained in co-culture of PBMC with autologous APC.
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PMC11803918
SOSG probe, Calcein AM, Hoechst 33342, MitoTracker, and DAPI were obtained from Thermo Fisher Scientific (MA, USA).
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PMC11576293
For C25H22N2O2SCl (M + H) 449.10850; found 449.10846.
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PMC11033180
All data reported in this paper will be shared by the lead contact upon request.
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PMC11098378
His+ colonies were selected on a medium lacking tryptophan, leucine and histidine.
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PMC11765243
At the DP stage, thymocytes rearrange the TCRα locus to express a mature clonotypic TCRαβ antigen receptor and may recognize self-peptide-MHC (p-MHC) ligand.
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PMC11407042
Observe the cell adhesion under the inverted microscope.
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PMC11093197
After incubation, cells were washed with PBS‐T and incubated with a secondary antibody at RT for 1 h in the dark.
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PMC11509309
To the best of our knowledge, this is the first report of the presence of metabolites 1–6 in Suberea laboutei species.
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PMC11536589
The protein levels of MGST1 in AsPC1-MR cells subjected to the stable knockdown of CTNNB1 (CTNNB1 KO) or TCF4 (TCF4 KO) were assessed by western blot.
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PMC11762280
The relative protein levels of the interested proteins were compared with ACTB as quantification (n = 3).
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PMC11139449
Two distinct sgRNA sequences were designed to target the 3’ UTR region of the CASP8AP2 gene using CHOPCHOP software.
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PMC5833712
Following SDS-PAGE, proteins were transferred to nitrocellulose, reacted with specific antibodies and then detected with peroxidase-conjugate secondary antibodies and chemioluminescent ECL reagent.
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PMC8567602
D, WB for BAF components after elution following IP-HA for SS18–SSX in HA-tagged HSSYII.
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PMC8409415
Significantly higher intensity score was observed in the after NACT group compared to that in the before NACT group (Figure 1C; median score 6 vs 2, P = .03).
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PMC9429973
The study included an initial phase I dose escalation portion followed by a phase II dose expansion.
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PMC9429973
To evaluate viability in group 1 we used a different ISHAGE protocol with lower thresholds.
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PMC9429973
Five LTE patients (31%) continued on rilzabrutinib monotherapy and 11 used concomitant medication (TPO-RA n=2 [13%], CS n=7 [44%], TPO-RA + CS n=2 [13%]; Figure); 1 patient used rescue medication.
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PMC11185260
Collectively, scL-SMARCB1 treatment enhanced radiation-induced cytotoxicity in ATRT, significantly inhibited tumor growth, and extended survival time compared to radiation treatment alone.
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PMC11763126
Furthermore, biotin-IP assay demonstrated the interaction between YTHDF2 and CBDP1 in ccRCC cells (Fig. 6E).
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PMC8524093
Using 4–6-week-old DBA/1J and AG129 mice, they infected them with 10 or 10 cell cytotoxic infectious dose 50 (CCID)/ml and 10 or 10 CCID50/0.1 ml, respectively, via subcutaneous route in the footpad and hocked of the right leg.
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PMC11597167
It is known that oleuropein can non-covalently bind to Aβ peptide to inhibit its polymerization and fibril formation; therefore, it could be considered a neuroprotective polyphenol .
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PMC5173112
To study the impact of USP21 on 786-O cell proliferation, we performed MTT and colony formation assays.
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PMC11448304
First, in HEK293T cells, we confirmed the differential Khib modification site of EGFR (Fig. 7a).
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PMC10976516
Moreover, systematic reviews and meta-analyses evaluated the efficacy and safety of OV in lung cancer and showed that the objective response rate was significantly higher in patients receiving oncolytic adenovirus H101 monotherapy or combination with chemotherapy than in patients only receiving chemotherapy.
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PMC11026382
The red, dashed inactive line marks the average relative growth rate for nonsense mutations (stop codons) in the first 120 positions of DHFR.
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PMC3888431
All available actions, that can be performed on the selected gene, are listed on the bottom right part of the main window.
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PMC9429973
Median age was eight years [range 3-15 years].
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PMC8873393
DNA repair-related XR-seq combined with RNA-seq from chromatin and cytoplasmic fractions can help decipher how transcriptional control contributes to DNA repair and vice versa.
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PMC6379402
To investigate the organizational principles of our networks, we perform model fitting experiments in which the wiring patterns of real-world networks are compared, using the above described GCD-11 distance measure, to the wiring patterns of randomly generated networks.
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PMC11791264
B, endogenous SETD7 interacted with endogenous LC3B in HEK293T cells.
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PMC8427838
Furthermore, considering our observation that higher expression profiles of TMIGD1, moesin and ezrin correlate with better survival, deeper investigation into the molecular basis of downregulation of TMIGD1, moesin and ezrin will shed new insight into the altered roles of cell adhesion signaling mechanisms in tumor progression.
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PMC11544122
To measure β-arrestin1 recruitment to CXCR4, cells were co-transfected with 50 ng of CXCR4-RlucII and 150 ng of β-arrestin1-GFP.
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PMC11306331
See Fig. S4A for cell sorting gating strategy.
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PMC11473843
Source data are available online for this figure. (
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PMC9436459
Cathayanon E (135) has a 6-membered oxygen ring formed by the intramolecular nucleophilic addition of 5-OH with the olefinic C-1", which was obtained from the stem bark of M. cathayana by Zhang et al. in 2009 .
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PMC11023271
After infection, HaCaT or A431 cells were selected under 1 μg/ml puromycin and resistant populations were amplified and used for further analyses.
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PMC9275501
For example, one mole of the natural active ingredients and one mole of the chemotherapeutic drug can be linked together by ester bonds or disulfide bonds.
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PMC10976516
Adenovirus and vaccinia virus are two examples of OVs that may be altered to carry transgenes (armed OVs), after which transgene products can be specifically delivered to the tumor microenvironment and further stimulate an anticancer immune response.
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PMC10958426
Light blue and blue represent fold change >1.5 and fold change >1.8, respectively. (
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PMC11583690
A comparison between BPMO@DTX and MSN@DTX reveals that the difference in cell viability starts to emerge from concentrations of 30 μg/mL and above (with p < 0.01), indicating that BPMO@DTX reduces toxicity on normal Hs68 cells compared to MSN@DTX.
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PMC10165258
The viability of cells within the spheroids was evaluated by adding PI (1 μM) into the primary droplet.
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PMC10507284
Seed cells according to the section Materials and Methods and detect the number of cells passing through the chamber after 48 h. (L) The quantification of results from transwell invasion assays (n = 3 for each group).
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PMC10631132
Ct values for CSF1R within the fibroblast-derived iPSC line were below the detection threshold.
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PMC11472639
Specific lysis of RPMI-8226 cells cultured with PBMCs and HI-FCS mediated by daratumumab (ADCCHI-FCS).
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PMC11632064
Spare respiratory capacity in human melanoma cell lines. (
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PMC10957991
Actomyosin contractility itself has a major role in the maintenance of intact epithelial junctions through regulation of tension at the cell–cell contacts.
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PMC11518994
No other known common pathogenic or likely pathogenic variants were identified through WES.
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PMC11194678
H and I) Representative time-lapse DIC images showing preformed STAU1 droplets (10 μM) could be dispersed with NaCl (H) or significantly reversed to aqueous phase with 1,6-HD (I).
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PMC11697703
While some isolated results did not reach statistical significance, they showed an effect consistent with the overall premise.
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PMC11224020
d, Heat map of genes harboring a similar expression pattern as Ccr7.
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PMC11697065
Given the metabolic changes that occur during the fed-batch process, it is perhaps not surprising that the dominant signature was time-point of cell collection.
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PMC11777207
Like the PD-L1 10F9G.2 antibody clone, the PD-L1 43H12 antibody clone resulted in lost PD-L1 staining as assessed by gMFI as a result of steric hindrance of the flow cytometry antibody with the blocking antibody (fig. S4B).
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PMC11225860
Live fluorescent imaging was performed via an Incucyte SX5 and images were taken every 30 minutes to track cell cycle progression.
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PMC11765988
According to another hypothesis, sarcoidosis induces regulatory T-cell amplification.
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PMC9878278
We used two siRNAs targeting exon 3 and exon 11 of DDX58 to block RIG-I mRNA expression.
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PMC9429973
Median DOT & PFS in PI-naïve/exposed/refractory pts were 20.4/15.2/7.6 mos & not reached/19.7/12.9 mos, respectively.
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PMC11321678
EOMES, which is a glutamatergic lineage‐specific transcription factor and marks unipolar brush cells (UBCs), is associated with a Group 4 cluster‐specific CCAN, indicating the putative cellular origin of Group 4 (Figure 2D).
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PMC9429973
Results: The study included 127 patients, who underwent HPCs collection for ASCT; 15% (19 patients) had failure to mobilize (63% men; mean age 48 years).
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PMC10170482
Hoechst 33342 (blue) dye was used to counterstain nuclei.
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PMC9429973
After revision of the diagnosis, BSS was confirmed in 20% of patients, WAS in 12.5%; some rare disorders were identified like MYH9-RD and Glanzmann thromboasthenia with macrothrombocytopenia.
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PMC11767793
Representative measurement data for the AuNPs@NIST and commercially sourced AuNPs (Au-PEI@C1, Au-PEI@C2, and Au-PEG-COOH@C1) are presented in Table 1.
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PMC11750161
Thus, the Rmax value did not show any specific variation, indicating that the phosphorylation regions in the eel LH/CGR C-terminal region played a pivotal role in the PKA signal transduction pathway.
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PMC7987994
It could, to a certain extend, mimic the circulation of body fluids in vivo.
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PMC11551844
It uses 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to assess cellular metabolic activity in terms of viability, proliferation or cytotoxicity.
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PMC7053076
For example imatinib, a known NF-κB inhibitor, reversed the acquired resistance to doxorubicin by down-regulating the level of ABCB1 through the inhibition of RelA (p65) activity .
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PMC9065483
Representative luminescence images are shown.
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PMC11033180
Figure 1Expression of Sema5A and its receptors Plexin A1 and Plexin A2 in rat brain and colon(A) Western blot of Sema5A from rat brain and distal colon.
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PMC10507284
B and C) The gene set with the highest accuracy was screened by the SVMRFE machine algorithm. (
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PMC11739484
This suggests that has-mir-22-3p miRNA is the most notable regulator of expression for MMP7, MMP11, and MMP14 genes.
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PMC9672323
The effect of dexamethasone and piroxicam was measured on the SOD3 activity (a) and TAC levels (b) of T cells in PBMCs (n = 3).
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PMC9429973
Aims: To compare cilta-cel with treatments from RWCP in terms of overall response rate (ORR), very good partial response or better rate (≥VGPR), progression-free survival (PFS), and overall survival (OS).
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PMC10914904
Moreover, vault particles can interact with SQSTM1/p62, contributing to the enhancement of the vault-phagy process Vault RNA 1-1 and Vault particles play a crucial role in the regulation of apoptosis and autophagy.
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PMC11725127
To identify the target of GA for its KRAS-reducing activity, we synthesized a biotin-labeled GA (Fig. 2A), which retained the ability to induce the degradation of KRAS in MM cells (Supporting Information Fig. S2A).
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PMC9599726
These data indicate that ferroptosis plays a role in VP-induced cell death and that iron is important in this mechanism.
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PMC11472639
Peripheral blood mononuclear cells were isolated from blood samples and incubated with the RPMI-8226 plasmacytoma cell line, with or without the presence of daratumumab to determine specific lysis using a calcein-release assay.
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PMC11519583
T-SNE and violin plots were drawn using the tool on the website.
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