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OTAR3088
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PMC11437637
Moreover, these findings indicate that in addition to blood pressure, glucose, and lipids, PBLs can also be used to predict EC risk in patients on medication to control underlying disease.
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PMC11805741
In the analysis of the binding interactions with the BCL-2 protein, the ΔG for astaxanthin and sorafenib revealed notable differences.
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PMC11075223
In the Qing Dynasty, Shengma Decoction had good activity on the treatment of severe headache accompanied with ringing in the head (thunder headache in TCM theories) recorded in Yifang Jijie .
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PMC11594074
All antibodies were purchased from Cell Signaling (Frankfurt a. M., Germany) and incubated overnight at 4 °C.
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PMC8193813
CO2 concentrations up to 10000 ppm were observed; the relatively lower average CO2 concentrations reflect the rapid mixing and entrainment of ambient air, causing dilution.
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PMC11483468
Therefore, protein inhibitor therapy targeting telomerase may provide a new safe and effective cancer therapy approach.
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PMC11541241
We provide emerging roles of the plant TOR pathway in plant–phytopathogen interactions.
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PMC11799620
The cell viability was determining according the following formula: The capacity of OFAE extract to protect exposed cells against oxidative damage enhanced by Cd exposure was evaluated by the method found by (Athmouni et al. 2018b).
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PMC7433824
Only the pro‐tumorigenic mitochondrial ornithine aminotransferase and anti‐apoptotic mitochondrial 60 kDa heat shock protein were downregulated in A172 cell line and U87 cell line when treated with P4, respectively.
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PMC9429973
Aims: To characterize the incidence and outcomes of isolated adult pLCH in the United States.
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PMC11720107
The cells were maintained at 37 °C in a humidified atmosphere with 5% CO2.
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PMC3931643
We also observed that VAL cells expressed the isoform 4EBP2, and that asTORi treatment did increase the amount of 4EBP2 bound to the cap complex.
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PMC11759751
We found that tumor growth was slower in the Vehicle + CD19 CAR T group and Chidamide + CD19 CAR T group than in the Vehicle and Chidamide groups (Fig. 5B).
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PMC9368503
In contrast, ontologies upregulated in cell lines versus patient tissue specimens comprised proteins involved in the regulation of lipoprotein particle clearance and RNA splicing.
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PMC11611077
Such DNA fragments were inserted into two mammalian expression vectors, pBApo-EF1α Neo DNA and pBApo-CMV Neo DNA (Takara Bio, Shiga, Japan), using In-fusion cloning kit (Takara Bio).
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PMC11089031
All those data indicated that ZNF692 negatively regulated G3BP2 and TM9SF2 expression in ccRCC.Fig.
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PMC10890307
Here, we evaluated the growth rates, the specific rates of glucose and glutamine consumption, and lactate and glutamate production to determine if lung tumor cells proliferate under stressful culture conditions associated with the tumor microenvironment.
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PMC6461034
Phosphosites are filtered for class I phosphosites (localization probability > 75%; Olsen et al, 2006), coupled to phosphopeptide spectral count data, and used for substrate‐centric inference of kinases on the basis of kinase–substrate relationships that are either experimentally observed (provided by PhosphoSitePlus, “PSP”) or predicted by an algorithm using sequence motif and protein–protein network information (NetworKIN, “NWK”).
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PMC10114490
A iHDF-T karyotype is not altered after more than 70 population doublings: 46XX,del (15) (q11.2q15).
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PMC10142392
PCL nanoparticles (about 300 nm) loaded with carboplatin (hydrophilic chemotherapy) were prepared for this purpose.
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PMC10196713
Thus, the extent of lipid peroxidation in OVK18 and OVK18cis cells treated with cisplatin was examined by flow cytometric analysis using the fluorescent probe Liperfluo.
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PMC11653533
Natural products are a vital source of new drugs, with many medications stemming from this highly-esteemed wellspring.
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PMC11718817
Videos of 30 min length were recorded at 4 and 18 h after the start of coculturing PancOVA spheroids with ex vivo generated OT-I CD8 CTLs.
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PMC11756907
A culture medium (Dulbecco’s Modified Eagle’s Medium, low glucose, Sigma Aldrich (Poland)), supplemented with 10% heat-inactivated foetal calf serum (FBS), enriched with antibiotics (penicillin [100 μg/mL]/streptomycin [100 μg/mL] was used for breeding (Sigma Aldrich, Poland)).
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PMC8348645
To this diluted mixture, hydrochloric acid was added.
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PMC4816271
Indeed this phenomenon of CARs containing IgG1 CH2-CH3 spacers has previously been reported .
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PMC9429973
Patients will be followed up from their end-of-induction treatment visit until the end of the study, including clinical evaluation every 3 months in the first year and every 6 months in the second year for patients undergoing ASCT and maintenance treatment.
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PMC11348300
For FaDu, BxPc3, and AsPc1 cell lines, tumors were imaged at 1 h, 3 h, and 6 h postinjection.
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PMC9516447
In contrast to pancreatic cancer, studies in non-small cell lung cancer showed that knockdown of mutant KRAS by itself may not be sufficient treatment due to compensatory oncogenic pathways, however this may offer opportunities to couple with other targeted therapeutic approaches .
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PMC11765879
The first layer consisted of RPMI-1640 media solidified with 0.4% agar.
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PMC11257203
Indomethacin, in addition to digestive tract ulcers, has other detrimental effects such as liver damage, kidney failure, blood abnormalities, and an increased risk of heart stroke (2).
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PMC9398137
To assess the practical significance of treatment effectiveness, we plotted charts with growth curves indicating group mean tumor sizes accompanied by expert opinion on the differences observed.
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PMC9429973
Duration of thrombocytopenia in CLV regimen – 9 days, LEAM and BeEAC -11 days.
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PMC11686380
In addition, because the transfection efficiency depends on the cell surface area physically exposed to the ventricular lumen where plasmids are injected, neurons incorporating a low amount of a vector are expected to undergo partial effects.
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PMC11806818
The sandwich structure was separated from the PSA mixture using the magnetic effect of the magnetic beads, and the PtNP@Co3O4 nanozymes were able to catalyze the redox reaction between H2O2 and TMB, which produced quantitative electrochemical and colorimetric reactions simultaneously in homogeneous solution.
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PMC11539788
Bladder cancer (BCa), one of the most prevalent malignancies of the genitourinary system, ranks ninth in incidence and thirteenth in mortality among all cancers .
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PMC10213199
Co-expression analysis and function enrichment of the HL60 cell line.
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PMC11792888
The effects of single and combined treatments on cell cycle phases in the A549 line were also analyzed.
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PMC9429973
Screening and data extraction were conducted independently and in duplicate by 2 reviewers.
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PMC9429973
Key secondary endpoints are changes in Hb level, markers of hemolysis, ATP and 2,3-DPG levels, and Hb-oxygen affinity (p50, Hemox Analyzer).
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PMC11680562
Pectin gels are usually created by a process called ionotropic gelation.
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PMC9577200
Besides, CS significantly (p < 0.05) inhibits bacterial growth in the range of 0.2–1.5% compared to control.
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PMC8164677
Patricia Cho et al. (2019) working at Harvard Medical School recently conducted a study in a 3D spheroid model where Nrf2 hyper-activation in lung cancer enabled the cancer cells to bypass the death program through enhanced antioxidant role of cancer cells; knocking down Nrf2 through CRISPR resulted in cell death in inner cells of spheroids which previously escaped death.35 Wedelolactone showed more inhibition of Nrf2 than standard as observed in silico (Table 3; Figure 9).
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PMC9684669
The statistically significance between two curves were analyzed by extra sum-of-squares F test.
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PMC11365983
Immunoblots demonstrate that overexpressing Foxc1 induces faster accumulation of Gli1 protein in serum-starved NIH3T3 cells.
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PMC6617630
BCL6: BCL6-F (5′TAACATCGTTAACAGGTCCATGACG3′) BCL6-R (5′GCCCCGTTCTCACAGCTAGAATC3′) GAPDH: GAPDH-F (5′GAAGGTCGGAGTCAACGG ATTTG3′) GAPDH-R (5′ATGGCATGGACTGTGGTCATGAG3′).
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PMC11693599
Taxane/platinum-based or neoadjuvant chemotherapy, in combination with surgical resection, is the first-line treatment for ovarian cancer (4).
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PMC9878562
Interfering with this axis by ACKR3 deletion impairs lymphoma cell migration towards CXCL12.
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PMC11627060
To complete the reaction, the solution was maintained at room temperature with constant magnetic stirring at 500 rpm for 18 h. The resulting NP suspension underwent centrifugation for 20 min at 12,000 rpm.
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PMC9429973
Aims: The aim of this phase 2 study is to evaluate efficacy and tolerability of Ixazomib-Daratumumab (I-Dara) without Dexamethasone in elderly frail patients with relapsed myeloma (RRMM) (NCT03757221).
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PMC11752767
Innovations in CAR design, transduction methodologies, and selection of the optimal antigens are bound to lead to improved responses and reach more patients with advanced melanoma.
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PMC11402217
Data was analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test.
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PMC9429973
Aims: To investigate the safety and efficacy of esomeprazole in treatment of iron overload in patients with non-transfusion-dependent hereditary anemias.
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PMC11471157
In the analysis of the labeling indices for Ki67 and PCNA, over 500 cells from each experimental group were counted under high magnification, and their rates of positivity analyzed.
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PMC11228511
Our studies are consistent with earlier studies showing that NM ruptures are more frequent in cells with a nuclear bleb (27, 30), presumably due to a weakened nuclear envelope (28).
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PMC11352666
The data for MERTK RNA expression in EWS patient samples were derived from the pediatric cancer data portal (PeCan) at https://pecan.stjude.cloud/ (accessed on 8 June 2023).
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PMC11637820
In contrast to previous investigations, using CAP, gram-positive bacteria were more sensitive to CAP treatment when compared to gram-negative bacteria derived from DFUs.
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PMC9429973
40% of patients received consolidation with autologous stem cell transplantation.
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PMC11525028
The purified protein was loaded into cell and the inhibitor into a syringe.
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PMC11628904
Larvae exposed to far red‐expressing C. albicans were imaged using a Nikon Eclipse TE300 inverted fluorescent microscope with a Plan Apo NA 0.45/10× objective.
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PMC11190062
TLR1–10 mRNA expression in CD138 cells isolated from MM patients (N = 772).
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PMC11576293
This experiment examines the impact of the newly developed compounds 9–29 on normal cell lines to assess their safety level.
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PMC11786704
Fig. 1 Synthesis and Characterization of PC Capsules. (
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PMC3734024
Histograms comparing surface expression of CD125 in CD19, CD19FasL and CD19FasL cells. (
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PMC9777812
In this study, we further validated the regulatory role of bta-miR-106b on CDKN1A expression, thereby promoting mammary gland epithelial cell proliferation and activating the PI3K/AKT/mTOR pathways to regulate milk protein synthesis.
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PMC10669128
Nonetheless, cancer cell lines present several limitations.
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PMC8711270
This is because CHO cells do not express the enzymes β-galactoside α2,6-sialyltransferase, α1,3/4 fucosyl transferase or β-1,4-N-acetylglucosaminyltransferase III, which are expressed in human cell lines (Goh and Ng, 2017).
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PMC11752767
TILs sorted by MATIC require only 5 days of expansion to reach the necessary cell numbers for therapeutic use, with a purity of up to 95% and an isolation efficiency of 90% .
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PMC11594074
Fifty poses were further refined with the GBVI/WSA dG scoring function and the receptor’s induced fit mode.
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PMC9429973
Recently, cell-free DNA (cfDNA) has been proven to resume the heterogeneity of spatially distributed clones.
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PMC9429973
Summary/Conclusion: Our study highlights that tissue-specific transcription factors regulate cell cycle exit at the promotor level and lineage-specification and terminal differentiation at the enhancer level during hematopoietic development.
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PMC10297204
In this stage, 45 animals were inoculated subcutaneously with HS-SY-II (1.0 × 10 cells per mouse).
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PMC11411004
Next, the cells were centrifuged at low temperature, and the supernatant was collected for subsequent experiments.
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PMC10093184
Several authors described spheroid formation in canine and human osteosarcoma cells, including D-17 and COS4288 cells, which was confirmed in the present study, and additionally demonstrated for the other COS cells .
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PMC11697065
The secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental bottleneck in the manufacturing of protein-based therapeutics.
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PMC11486946
Solid lines correspond to the indicated compound.
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PMC8524093
Among neurological complications, the most prevalent symptoms seem to be abnormal mental status, headache, focal deficits, and seizures.
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PMC10006224
Four different samples were stained with increasing dilutions of Pacific Blue (Invitrogen) for 30 min in the dark at RT and then mixed into one tube.
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PMC9429973
Real-world data of patient outcomes post cBTKi and BCL2i exposure in CLL/SLL are limited to small retrospective studies, and the optimal treatment in this setting is unknown.
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PMC10125312
Viability of cell lines for all studied concentrations shown as percent of controls (0 nM). (
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PMC10213179
injection of fluorescent-labeled aptamers with cyanine 7 (Cy7): Cy7-ATOP or Cy7-SCR at a concentration of 1.6 mg/kg.
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PMC10788478
Pathway analysis was also conducted based on the mRNA sequencing data for both HCC15 and A427 cell lines (Tables S8 and S9), and “G‐protein coupled receptor signaling” (z‐score: −2.840 in HCC15; z‐score: −2.502 in A427), “Cardiac hypertrophy signaling (enhanced)” (z‐score: −2.111 in HCC15; z‐score: −2.065 in A427), and “Pulmonary fibrosis idiopathic signaling pathway” (z‐score: −2.309 in HCC15; z‐score: −2.065 in A427) were the commonly regulated pathways (Table 5) which are potentially associated with the progression of lung cancer exhibiting LGR6 overexpression through the Wnt/β‐catenin pathway activation.
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PMC11551844
AuNPs-JC-2 showed enhanced cytotoxicity with a decrease in IC50 values from 3.37 ± 0.19 μg mL to 0.52 ± 0.09 μg mL in A172 and from 2.28 ± 0.20 μg mL to 0.78 ± 0.28 μg mL in TERA1 compared to JC-2.
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PMC11746948
Our study initially aimed to identify critical metabolic regulators that affect ferroptosis sensitivity.
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PMC11483468
In addition, apoptotic cells were observed, which became small and round with fragmented nuclei (Figure 4C, yellow arrows).
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PMC9592219
Taken together, our study unravels the hitherto unappreciated role of TOP2A in the Hippo signaling pathway.
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PMC9429973
With a median follow-up of 26.5 (1.2-239) months, relapse was observed in 16 patients, 4 of whom presented only with isolated extramedullary disease.
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PMC11705484
Repeated measures analysis of variance (ANOVA) was used to analyze mRNA stability at different time intervals for each group separately. *
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PMC10170482
Labeled cells were then imaged by confocal microscopy.
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PMC11621493
The protein-containing fractions were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (732-2010, Bio-Rad).
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PMC11769516
In summary, the studies conducted by Agahi et al. show that the antioxidant enzyme system in SH-SY5Y neuroblastoma cells plays a strong protective role against damage caused by α-ZEL, β-ZEL and BEA mycotoxins both when combining the discussed mycotoxins and in a single study .
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PMC11597167
This cleavage event generates a soluble fragment called β-C-terminal fragment (β-CTF), which is further processed by γ-secretase to produce Aβ peptides .
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PMC11687663
In Bangladesh, the rhizome of A. helferiana was used in dysentery, infections, scabies, and muscular aches.
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PMC8711270
In retroviral vector production, HEK293T is used due to the expression of the SV40 large T-antigen in the cell line.
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PMC11670407
Statistical analysis of fluorescence intensity was performed using FlowJo V10 software (n = 3).
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PMC11525028
Cell viability was measured daily for four days following the manufacturer’s instructions.
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PMC11411131
In addition to MeHg, electrophilic stress activates Nrf2-mediated transcription, which upregulates cellular defenses against toxicants.
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PMC11489351
For these reasons, MCF10A cells are an ideal model of normal human epithelia to characterize the pHi-dependent transcriptome.
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PMC8973725
The morbidity of RCC is rising by 2–4% each year .
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PMC11615218
Age-related senescence involves telomere shortening, whereas premature senescence is telomere-independent.
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