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OTAR3088
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BioData-CellLine_annotated

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PMC11772449
The absorbance at 450 nm was read by a microplate reader.
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PMC11745823
The protein sample was mixed with loading buffer and heat‐denatured, loaded onto an SDS‐PAGE gel, and electrophoresed to separate the proteins according to their molecular weight.
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PMC11742047
4Lipidomic results HL-60 cells.
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PMC11243198
In the study by Calcaterra et al. , it was proposed that the di-protonated form of GANT61-D would be the prevalent form under physiological conditions.
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PMC9100622
The measure of the surface area of spheroids was normalized to spheroids treated with DMSO.
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PMC10538569
Human primary CD4 T cells or primary GPI-scFv X5 transduced CD4 T cells were cultured alone or co-cultured with autologous human primary CD8 T cells or primary GPI-scFv X5 transduced CD8 T cells, and then infected with HIV-1 NL4-3.
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PMC6835428
Despite this, CD44 does not seem to have relevant prognostic value in OC .
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PMC11798926
These results provide important insight into the mechanisms by which SynDIG proteins are targeted to endosomal compartments as a step in understanding SynDIG-mediated regulation of AMPAR trafficking.
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PMC11285179
The percentage of pericytes was 1.09% in LLC-NC and rose to the rate of 2.14% in LLC-CCL28 (Fig. 5D).
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PMC11422497
OD, Optical Density.
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PMC11319300
Interactions where the LTO1 promoter was taken as bait are shown in blue.
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PMC11700247
E-F) Expression of ifna1, inb1, cxcl9, and cxcl11 48 hours post in vivo RT. (
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PMC11279598
One target protein is the DNA repair enzyme poly(ADP-ribose) polymerase (PARP).
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PMC7812570
B The ratio of gp120 to gp160 in cell lysates was determined by quantifying the corresponding band intensities with ImageJ. The data shown were derived from three independent experiments, and error bars indicate standard deviations.
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PMC11623887
In infected cells besides NS1, B19- 11kDa protein is involved in the induction of cell death.
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PMC11518702
Within primary human dermal mast cells, FcϵR1 ligation induces rapid increases in C1ORF150 transcripts (19) ( Figure 2B , center panel).
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PMC9429973
Our data also confirms the relatively quiet landscape of accompanying mutations in KMT2A-rearranged AML with RAS pathway most frequently involved.
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PMC11656657
Data are represented as mean ± standard deviation (SD) from n = 3.
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PMC2614415
Confirming flow cytometry data, combination treatment with paclitaxel and VE-465 did not synergistically increase apoptosis in the PTX10 cell line. (
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PMC6133382
Mixed results were seen for the PGT121 family of bN-mAbs in which the binding to gp120 produced in both the MGAT1 CHO and HEK 293 GnTI cells lines was lower than binding of these antibodies to gp120 produced in the CHO-S cell line.
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PMC11585565
Our previous research identified a missense variant in CNNM4, c.1475G > A, p.(Gly492Asp), among the Lua ethnic group of Northern Thailand.
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PMC11617590
Effects of sulforaphane on GSK‐3β and β‐catenin expression. (
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PMC11591052
The representative images of Western blots that were repeated three times with essentially the same results (see the graphs in the figures) are demonstrated.
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PMC11751675
The ongoing exploration of SLs will likely yield important advancements in antiviral drug discovery, positioning these compounds as key candidates in the fight against viral infections.
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PMC11754291
This is further validated by photomicrography (Figure 9), which visually confirms increased pigmentation corresponding to elevated tyrosinase and DCT levels (Figure 8).
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PMC11750712
Day 123 was the latest time point in which CAR T cells were detectable by ddPCR (70 VCN per μg DNA; limit of quantification of 20 copies per μg DNA).
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PMC4277423
The resulting vectors were designated as pRRL-sgRNAs (CR1, CR2, CR3 or GF1).
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PMC10568263
Next, the mixture solution was stirred for 30 min at room temperature and transferred into the Teflon-lined stainless steel autoclave.
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PMC5363531
The phospho-mTOR content was semi-quantitatively assessed and mTOR phosphorylation was slightly increased following the administration of single agent (PEM, dasatinib) or in the concomitant administration, but decreased in sequential administrations (data not shown).
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PMC9503669
Use of this Nef-transfected cell line separates the effect of Nef on MHC-I from effects on viral infectivity and replication, thus simplifying interpretation of the results.
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PMC9429973
These Vav1-driven STAT5B transgenic mice develop severe CD8+ T-cell malignancies.
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PMC11240571
Next, after the tumor grew to about 5 mm in diameter, the mice were irradiated with 12 Gy.
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PMC10490530
For immunoblotting, 40 μg of protein were resolved by SDS-PAGE, transferred onto BioTrace PVDF membrane, and probed using appropriate dilutions of the following primary antibodies as listed in Supplementary Table S3.
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PMC9429973
Methods: A 34-year-old woman with B-ALL was assessed at the genetic level.
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PMC11626627
Data are presented as mean ± SD. *
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PMC11769516
In the plasmid disruption assay, no DNA damage was observed at specific time points of 6, 12 and 24 h. Apoptotic nuclei were demonstrated in DAPI staining after 12 and 24 h. SH-SY5Y cells treated with the mycotoxin ZEA showed a clear inhibitory effect on the expression of neuronal genes .
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PMC11543935
A study has shown that tumor cells have strong repair mechanisms against membrane disruption due to ESCRT processes (Endosomal sorting complexes required for transport).
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PMC10940855
EFNB2-mediated activation of EPHB2 or EPHB4 forward signaling has also been shown to augment EC chemotaxis in response to SDF-1 (73).
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PMC11411131
Consequently, combinations of pCMV-Krab-Sec and pCMV-Tre-Luciferase (pCT-Luc) were chosen.
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PMC11743316
Because mitochondria perform a variety of tasks in cancer cells, targeting specific molecules in the mitochondrial or mitochondria-related pathways, such as TCA enzymes and OXPHOS-related genes, may offer new and potential treatment methods.
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PMC11026382
TYMS color coding follows from (a–d).
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PMC8427838
The most striking effect of TMIGD1 on the microtubule stability was observed at 20–30 min after nocodazole treatment (Fig. 5C).
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PMC8345486
The reaction mixture was stirred at room temperature for 24 h and the reaction progress was monitored by TLC CH2Cl2/MeOH (9:1).
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PMC9429973
It affects particularly the skin, liver, and gastrointestinal tract (GI).
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PMC10719213
Cladribine (2-chloro-deoxyadenosine, 2-CdA), a nucleoside analog of deoxyadenosine, is resistant to adenosine deaminase (ADA), allowing its accumulation in cells .
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PMC11423992
0023488, TOSOH) according to the manufacturer’s protocols.
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PMC11633763
Tumour‐suppressive effects of USP22 silencing in osteosarcoma cells partially reversed by HK2 overexpression. (
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PMC10882807
2.5 µg/mL Actinomycin D (Merck, Germany) was added to cells and cultured for 8 h. TRIzol reagent (Invitrogen, USA) was added at 0, 2, 4, 6, and 8 h to collect RNA.
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PMC10047551
However, a correlation between PD-L1 mRNA expression and the methylation levels was not apparent (Figure S2A,B).
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PMC5034105
The highest mean viral loads (⩾10 lgcopies/mL) were observed in the nonhuman primate (Vero and LLC-MK2), pig (PK-15), hamster (BHK21) and chicken (DF-1) cell lines.
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PMC10387886
RNA isolation (ThermoFischer, K0732) and cDNA synthesis (ThermoFischer, 4368814) were carried out following the manufacturer’s instructions.
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PMC9429973
Aims: In order to further explore the combined application of eltrombopag and first-line drugs, the efficacy and recurrence rate of the combination of eltrombopag and prednisone in the treatment of ITP were evaluated in our experiment.
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PMC11413393
It remains unclear whether this decreased expression is occurring via effects on RBPs, or other mechanisms such as transcriptional regulation via the H4R3me2a histone mark.
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PMC11748335
Considering the propensity of H3K27me3 to be distributed in regions with low DNA methylation , we analyzed the genomic relationship between LOCKs and common PMD/HMD regions as previously annotated .
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PMC11411131
Selenoproteins contain selenocysteine (Sec) residues, often referred to as the 21st amino acid.
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PMC11012313
For many oncogenic viruses, oncogenic transformation occurs years later and via numerous mechanisms.
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PMC11269792
CDV3 was higher in both GIST tissues and cell lines than in normal tissues and cell lines (Fig. 5D, E).
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PMC10933092
mA levels in sh-NC and sh-TRMT61A 5637 cells, as examined by RNA dot blot.
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PMC11424574
shLuc- and shMTA2-143B cells (1 × 10 /0.1mL) were injected into the tail vein of C.B17-SCID mice for ten weeks; mice were then sacrificed and the lung tissues removed, shLuc and shMTA2 143B lung tumor excised from C.B17-SCID mice. (
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PMC8041314
Supplementary Table 3 provides all 1049 classified compounds with names and SMILES codes.
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PMC11388384
Using total internal reflection fluorescence (TIRF) microscopy, we observed strong signal on the sender cell membranes, and extracellular particles that formed a concentration gradient extending ~50 to 100 µm from the boundary of the nearest sender cell, consistent with extracellular diffusion of SHH-Halo (Fig. 2 A and B).
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PMC9243326
We compared the predicted protein sequences with the Animal TFdb database using the hmmscan software and identified 658 TFs from 48 TF families.
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PMC11790971
It is worth noting that the importance ranking of imaging modalities was calculated based on four specific CHO cell lines.
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PMC11655536
Cells were washed twice with cold PBS and then resuspended with 1× Binding Buffer, followed by adding with 5 μl Annexin-V and 10 μl propidium iodide (Yeasen Biotechnology) in 100 μl cell mixture (1 × 10^5 cells).
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PMC11519567
Centrifuge cell lysate at 12,000 × g for 10 min at 4 °C.
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PMC10847511
For an unbiased evaluation, we analyzed all such assays separately without any modification.
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PMC11512230
Thus, the adsorption effectiveness of AltMV SPV and TMV SP on sarcoma cells was comparable.
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PMC11680562
Additionally, peaks at 1598 cm and 1384 cm signify the bending vibrations of the N–H group of primary amine and the C–O–H group, respectively, suggesting the involvement of these functional groups in the NP's surface chemistry.
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PMC11547972
To evaluate clonogenic tumor growth, 500 cells/well were transferred to 6-well-plates.
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PMC9429973
Our group of patients had reduced OS and PFS compared to the GALLIUM trial and SARS‐CoV‐2 infection was the most pronounced risk factor for death.
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PMC11512230
The cells were seeded into the plate within 24 h (RPMI or DMEM supplemented with 10% FBS str/pen, working volume 500 μL, and cell concentration 20,000 cells per well).
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PMC11802929
This question is not just of fundamental importance to T cell immunology, but it is also highly relevant to the engineering of T cells for use as therapeutic agents for treatments of both cancers and autoimmune diseases.
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PMC11513571
Quick Cell Proliferation WST-1 Assay Kit was used to evaluate the B cell survival after 4 days. (
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PMC11513011
DIB had no significant apoptosis-inducing effect on 5637 cells.
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PMC11653533
It has numerous therapeutic employments and is traditionally employed for its therapeutic benefits as a metabolic, cardiovascular, respiratory, antispasmodic, and anti-inflammatory cure (Ahmad et al., 2013, Gilani et al., 2008).
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PMC11711663
Kaplan-Meier (KM) curve plots for progressive free survival (PFS) at 12 months, 24 months, 48 months, and 60 months are presented in each row in Fig. 1.
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PMC9429973
TLR4 also was upregulated and positively correlated to mH2A1 expression.
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PMC7757104
The thermocycling conditions were as follows: Initial incubation at 50°C for 2 min and 95°C for 10 min, followed by additional incubation at 94°C for 5 min; 40 cycles of 94°C for 30 sec, 60°C for 30 sec and 72°C for 45 sec; and final melting curve analysis at 72°C for 15 sec, 90°C for 5 sec, 55°C for 5 sec and 90°C for 5 sec. Fold-changes in the expression levels of each mRNA was calculated using the 2 method (23).
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PMC11519788
Absorbance at 490 nm was examined using a microplate reader (BioTek Instruments, USA).
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PMC9429973
Three patients experienced infusion-related reactions (IRR) to the first dose of IV daratumumab.
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PMC11779605
The number of total genes in each pathway is depicted to the right of the chart.
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PMC11612794
Some kinetochore components have similar functions and simultaneously recruit the same proteins, making it difficult to investigate the significance of each interaction in cells.
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PMC6364197
Next, we chose DDR1-IN-1, a potent and selective DDR1 receptor tyrosine kinase inhibitor (21), to further analyze whether DDR1 inhibition would reverse the chemoresistance.
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PMC6799808
The results showed that treatment with 0.5 and 1.0 mg/ml GLPT for 72 h significantly reduced the percentages of viable cells from 95.2% (in cells treated with DMSO) to 77.0% and 45.1%, respectively (Fig. 1f, g).
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PMC11360263
All in all, anti-PGCC therapeutic development could enhance clinical outcomes, especially given the correlation between PGCC abundance and high tumor grades.
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PMC11656657
Xenograft studies were performed using female or male NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ; RRID: BCBC_4611 from Charles River) mice, 6 to 8 weeks of age.
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PMC11754094
Amplified products (1 µg) were purified using 0.95× AMPure XP bead-based reagent (Beckman Coulter).
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PMC10376064
Calcein AM: Fluorescence (excitation: 485 nm; emission: 520 nm) was measured for 30 min at 30 s intervals using a Paradigm microplate reader (Beckman Coulter Biomaterials, Munich, Germany); Daunorubicin: Fluorescence (excitation: 488 nm; emission: 695/50 nm) was measured after 180 min incubation on an Attune NxT flow cytometer (Invitrogen, Waltham, MA, USA); Rhodamine 123 and pheophorbide A: Fluorescence (excitation: 488 nm; emission: 695/50 nm) was measured after 120 min incubation on an Attune NxT flow cytometer; Hoechst 33342: Fluorescence (excitation: 360 nm; emission: 460 nm) was measured after 120 min incubation using a Paradigm microplate reader.
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PMC2196094
A cDNA clone containing the entire coding region for the rat tryptase RMCP-6 was isolated.
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PMC9429973
ATG was second-line therapy in 40pts and third line in 3pts, with a median of 5 doses (1-5).
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PMC11055323
SS, SS18-SSX1 SS18-SSX1 excludes HDAC1/2 complexes from condensates.
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PMC9429973
The rate of CRS was 90% (8% grade ≥3, 1 grade 5), and the rate of ICANS was 61% (32% grade ≥3, 0 grade 5).
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PMC5261804
Of particular interest, the PPIs among the top 20 most essential often those linked proteins that dominated particular PPI clusters such as AKT1-SRC, AKT1-MTOR, and CTNNB1-SRC.
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PMC3888431
The number of multiple gene loci in a single cluster was estimated by mapping the transcripts back to the reference genome and counting of gene loci for each cluster.
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PMC9118379
Using a similar concept, we stratified the evidence levels of variant peptides based on their spectral counts as follows: one PSM, two PSMs, and multiple (≥3) PSMs to classify variant peptides, denoted by 1_PSM, 2_PSM, and 3up_PSM, respectively.
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PMC9676986
Surprisingly, the deletion of E18 played a critical role in oncogenicity, whereas full-length variants marginally affected tumorigenesis.
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PMC11765243
Recombinant cytokines were from Peprotech-ThermoFisher Scientific (human IL-2 and mouse IL-4, IL-6, IL-12 and IL-21) or R&D (human TGF-β).
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PMC11468365
This model achieved a 4.0% improvement in compared to AuDNNsynergy.
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PMC11300639
The degree of binding was evaluated by multiplying the intensity score by the proportion of cells stained.
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PMC10565698
N. Changes in EMT pathway-related proteins in HCCLM3 and BEL-7407 cells under different transfection conditions were detected by western blotting. **:
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