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PMC9429973
HR = 1.23 (80% CI: 0.72, 2.10), p-value = 0.63].
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PMC11201245
Ninety percent arise from the surface epithelium of the ovary and represent epithelial ovarian carcinoma (EOC) .
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PMC11781181
Interestingly, miR-375-3p can also induce ferroptosis by targeting solute carrier family 7 member 11 (SLC7A11), a negative regulatory factor of ferroptosis, and thus attenuate the stemness of gastric cancer cells and cervical cancer progression 20-22.
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PMC11765988
Furthermore, sarcoid-like reactions (SLRs) highlight the possibility of paraneoplastic manifestations and stress the importance of distinguishing confirmed sarcoidosis from granulomatous responses associated with malignancy.
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PMC11342785
Stronger MT interaction strength allowed for some synMAP-driven bundling even in the absence of direct targeting to a droplet hub.
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PMC10577955
To dissolve the formazan crystals, 200 µl of DMSO (dimethylsulfoxide) (Sigma Aldrich, USA) was added to each well.
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PMC11354239
Upon degranulation, the CD107a becomes exposed on the surface of the NK cell.
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PMC6771295
Protein levels were analyzed using antibodies specific for the SIRT1, SIRT3, or SIRT5.
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PMC11697189
Total protein was extracted from ccCRC cells using RIPA buffer (Beyotime, Shanghai, China) with a supplementary protease inhibitor (Proteintech, Wuhan, China).
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PMC10588957
Several studies have demonstrated the tumour suppressor function of AMPK in lung, colorectal and liver cancers (23).
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PMC10705095
Notably, our results demonstrated the expression of hemoglobin in these lung adenocarcinoma cell lines.
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PMC11224206
Chart the response of the model to environmental, notably, heat stress.
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PMC11607321
Feature importance aggregated across all the samples and output dimensions can be found in Supplementary Data 9 and 10.
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PMC9429973
CMV reactivation was the 3rd complication (38%) and it appeared early (median 5 weeks after infusion).
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PMC11756514
The role of SP-D is aggregation and opsonization of pathogens, but it is also crucial for maintaining alveolar integrity.
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PMC11650848
DNA methylation across all CpG sites were varied in our samples, ranging from 4.33% to 100% with an average methylation of 63.25% (SD = 26.24).
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PMC11799620
After 24 hours of cell seeding, HepG2 and HEK293 cells were exposed to different concentrations of OFAE (50, 100, 150, 200, 300, and 400 µg ml) and CdCl2 (25 to 60 µM).
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PMC4369959
The induction of apoptosis was associated with upregulation of p53 and p21 proteins as well as production of reactive oxygen species (ROS) in zerumbone-treated PANC-1 cells .
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PMC9429973
A total of 14 patients died.
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PMC11295144
Images were acquired using Zeiss ELYRA PS.1 SIM at 63 x magnification.
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PMC10125312
Under the laser conditions used, we can confirm that the laser irradiation settings employed are nontoxic for these cell lines in vitro but, in cells with AuNR-PEG, maximum temperature, mitochondrial activity and membrane integrity differed between cell types.
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PMC9429973
Monotherapy doses between 1 and 42 mg were administered orally 3 days/week (wk) (3 wk in a 28-day cycle) and at 21 mg (4 wk in a 28-day cycle).
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PMC11486946
The coefficient of variation (CV), signal/background (S/B), and Z’-factor were respectively defined as the ratio of the standard deviation (SD) and average of the controls, the ratio between the averages of the positive (signal) and negative (background) controls, and the following equation:1[12pt] $$^ }=1-(3 }_}+3 }_})/(}_}-}_})$$′=1−3×SDpositive+3×SDnegative/Avgpositive−Avgnegativewhere SDpositive, SDnegative, Avgpositive, and Avgnegative represent the SD and average for the positive and negative controls, respectively.
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PMC10958426
and Single Cell 3ʹ Reagent Kits v3 for in-house data.
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PMC11765879
The thoroughly dried leaves were ground by a mill until obtaining plant powder.
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PMC11443022
Intravesical sperm collection was challenging due to difficulties in urinary catheterization caused by urethral stricture following urethroplasty.
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PMC11670407
Cells were treated with cisplatin for 24 h (5/10 μM for 143B cells and 10/20 μM for U2OS cells).
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PMC11665555
All experiments were performed with a minimum of 3 biological replicates.
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PMC11509309
Colonies formed a red halo if the product spotted on a filter had anti-prion activity, but remained white around the filter if the molecule was inactive against yeast prions.
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PMC11672142
The UV–vis spectrum for the Fe(triapine) (10) (50:50 DMSO:H2O) shows a LMCT absorbance at 500 nm (ε = 3000 M cm based on compound concentration).
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PMC11462673
Additionally, we further assessed the impact of LDB1 on cell proliferation using Jurkat, 6 T-CEM and J.gamma1.
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PMC9429973
Cases were selected to be representative of t(12;21), t(4;11), t(9;22) or t(1;19) subgroups, as well as MRD-based standard-risk (SR), medium-risk (MR) and high-risk (HR) response.
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PMC10965680
The cross-population selective sweeps in the Xiangdong black goat were determined by placing the Xiangdong black goat population as the target, and other goat populations in the dataset were placed in the reference group.
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PMC9429973
Results: Of the 1563 enrolled outpatient COVID-19 survivors [974 M/589 F, median age of 60 years (35.5-81)], 56 subjects [43M/13F; median age 62 years (42-80.5)] had had VTE during COVID-19 acute phase (3.58%), as follows: 9 isolated proximal DVT (4 in upper extremity), 45 isolated PE (34 subsegmental and/or segmental PE; 11 central pulmonary artery thrombosis), 2 DVT+PE.
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PMC8956657
To compensate for this loss of binding, we observed that the side chain of Arg, positioned two residues before where a P2 Lys would normally be, was bound at this pocket as can be seen in Fig. 2C that presents an overlay of ORF4b and SV40 T-ag NLS structures.
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PMC10747139
BOLD-100 has also recently gained particular interest for its potential multiple activities.
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PMC9841531
Determination of intracellular pH was carried out using the pH-sensitive dye 2′,7′-bis-(2-carboxyethyl)-5/6-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Sigma-Aldrich, St. Louis, MO).
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PMC7351993
Third, the number of pixels can be increased by adding photodetection circuits, lock-in amplifiers, and digitizers (Fig. 1, Supplementary Fig. 4).
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PMC9429973
Besides individual dropouts of the different samples, also shared dropouts were detected.
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PMC10297204
In vivo, both 30% and 99.5% ethanol concentrations, compared to 0% concentration, significantly reduced the local recurrence.
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PMC11637820
If not intervened upon, DFUs have the potential to result in severe complications, such as sepsis, abscess formation, the necessity of amputation, osteomyelitis, and gangrene.
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PMC10507284
The SW982 cells were plated at a suitable density in a six‐well plate for culture.
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PMC9429973
The area under the curve (AUC) or the distance between the starting point in the hypo-osmolar region (Omin) and an ending point in the hyper-osmolar region (Ohyper) that partially depends of red cell deformability Results: Exception made of HbE, in all the structural hemoglobinopathies and thalassemia the tail of the osmoscan curve shifts to the left suggesting the existence of a decreased osmotic fragility and dehydration.
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PMC5925824
In particular, we considered that positions 63 and 69 - side chains, which are exposed to solvent according to PDB: 5C3T , might be pivotal for the species specificity of LY3300054 because their corresponding amino acid substitutions diverge among the three PD-L1 sequences (marked with * in the alignment of Fig. 2a).
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PMC11742231
The adaptive nature of the technique ensures that the fusion process is tailored to the specific input data and task, making it more effective and efficient than fixed fusion methods.
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PMC11551844
AuNPs-JC-2 displayed an IC50 value of 6.81 ± 1.00 μg mL on the HaCaT cell line.
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PMC10870498
The LinkedOmics database (https://www.linkedomics.org/login.php) showed genes positively and negatively related to KIF23.
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PMC11723947
For the in vitro experiments, Treg were enriched by positive selection on MS column (Miltenyi Biotec) following staining with APC-conjugated anti-Thy1.1 antibody (OX-7, BD Biosciences) and incubation with anti-APC microbeads (Miltenyi Biotec).
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PMC10411253
Both variants resided in the N-terminal domain.
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PMC11796104
Primer sequences are provided in Table 2.
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PMC11803149
n = 13 (mice transferred with aged cells), n = 17 (mice transferred with young cells), n = 12 (PBS).
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PMC10212663
Receptor activator of nuclear factorκB ligand (RANKL) has been demonstrated to drive malignant phenotypes in lung cancer; however, its role in KRAS‐mutant (mt) lung adenocarcinoma (LUAD) is not yet fully elucidated.
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PMC9429973
The diagnosis was performed by skin biopsy of an extensive ulcerated mass in the right chest area.
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PMC11753949
Moreover, as discussed above, actin binding proteins that we had previously found to be components of the spine apparatus, such as Pdlim7 and α-actinin 2, colocalized with synaptopodin-ER when co-expressed with this protein in live COS-7 cells, suggesting that they are part of the dense matrix that connect cisterns of SALs (Figure 6B–C).
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PMC11394730
Researchers examined emodin’s anticancer efficacy using animal models and the hepatocellular carcinoma cell line SMMC-7721.
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PMC9429973
We here show specific proteomic signatures of proliferation and ER stress to be present in subsets of MPN patients and translate this to specific biological alterations and correlated therapeutic vulnerabilities.
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PMC11731161
D Quantitative analysis of tumor weights in the different groups.
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PMC9429973
Image: Summary/Conclusion: Our data unveil how dysregulated deubiquitination controls signaling pathways, leading to cancer cell survival and drug non-response, and suggest novel therapeutic combinations towards targeting T-cell leukemia.
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PMC10490530
The absence of rare codons in the hLuz DNA sequence was reconfirmed using the GeneOptimizer toolbox .
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PMC9429973
Results: Prmt2 mice do not exhibit difference in bone marrow progenitor or mature cell populations compared to wild type mice, indicating that PRMT2 has little or no influence in the hematopoietic stem cell maintenance.
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PMC11047729
Studies on U937 and HL-60 cells display the inhibition of growth and the induction of apoptosis after the use of this substance.
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PMC11021472
Thousands of circRNAs have been identified in eukaryotes through RNA sequencing and bioinformatics analyses, which have focused on the identification of reads containing BSJ sequences.
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PMC9927933
We also included Kras(–)Ube2c mice as a negative control.
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PMC11297139
The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes.
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PMC11093197
This study was supported by Grant‐in‐Aid for Scientific Research (17K14992 and 20K07612 to SK; 22K07209 to DV; and 17H03576, 17K19586, and 19K22555 to CT) and Grant‐in‐Aid for JSPS Fellows (23KJ1033 to LG) from MEXT; Funding Program for Next Generation World‐Leading Researchers (LS049) from the Cabinet Office of Japan; Project for Cancer Research and Therapeutic (P‐CREATE) (19cm0106164h0001) from the Japan Agency for Medical Research and Development (AMED); and TaNeDS (C1010568) from Daiichi‐Sankyo.
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PMC11725127
We first screened a homemade compound library to identify the compounds that can reduce the level of KRAS using MM cell lines, MM.1S and RPMI 8226, both of which harbor KRAS activating mutation.
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PMC9429973
The median (range) total duration of response for ≥33% and ≥50% responders (rolling 12-wk interval) in the luspatercept group was 586.0 (84–1300) and 357.5 (84–1267) d, respectively, and 171.0 (84–627) and 169.0 (84–485) d in the placebo group.
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PMC8303372
As shown in Figure 7a and in Table 1, the intracellular concentration of GA is statistically higher in the non-conveyed form than in the conveyed form.
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PMC10919056
Patients exhibiting concurrent low expression of GJA5 and GJB1 had the shortest OS, while those with high expression of GJA5 and GJB1 had a prolonged OS (Figure 3G).
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PMC8345486
Coupling constants are reported in Hertz (Hz).
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PMC9688728
Moreover, glutathione is the most commonly elevated small molecule detected during oxidative stress .
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PMC11487720
In negative controls, empty pSos and pMyr did not support yeast growth at 37 °C.
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PMC11055510
The results of wound healing assay showed that the cell healing rate in the ov-circMAN1A2 group was significantly higher than that in the ov-nc group.
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PMC7759933
The most important enzymes from phase I reactions belong to the superfamily CYP450 and play an important role in cellular metabolism and homeostasis, and detoxification and metabolic activation of xenobiotic compounds into reactive metabolites .
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PMC9429973
Evidence suggests that, even with support, a substantial proportion of patients with R/R SAA die within 5 years of diagnosis.
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PMC9429973
The other 2 patients without subsequent HSCT (all had a prior transplant) died from infection on day 78 and GVHD on day 103, respectively, post KO7CAR.
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PMC11782508
B. subtilis displayed the highest susceptibility to azurin with an inhibition zone of 23.0 mm, while K. pneumoniae was the least affected strain with an inhibition zone of 18.0 mm.
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PMC11357960
Based on the distribution of percentage change data, we roughly divided it into five groups, which are >100, 50~100, 0~50, 0~-50, -50~-100.
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PMC11770199
S6A and B).
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PMC10243817
Species- and subtype-matched Abs were used as negative controls.
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PMC11541241
Scientists failed to detect enhanced susceptibility to B. cinerea in the Arabidopsis TOR overexpression plants, whereas TOR inhibition showed enhanced resistance to B. cinerea in the Arabidopsis raptor1‐1 mutants and tomato (De Vleesschauwer et al., 2018; Marash et al., 2022).
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PMC11012313
We then tested if phospho-p70 S6K was required for the increase in HIF-1α levels in LMP2A-expressing cell lines.
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PMC11806182
Leave-one-out analysis was performed to assess whether any single SNP disproportionately influenced the overall causal estimate.
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PMC11046454
In addition to membrane marker expression, the secretion of proinflammatory cytokines, including tumor necrosis factor (TNF), IL-2, and interferon-γ (IFN-γ), were significantly enhanced by incubating AptPTK7-CAR-T with target PTK7 CEM, while little cytokine secretion was observed in the control group of Lib-CAR-T (Figures 2D and S17).
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PMC11790971
And the mean of average PPP per cell (i.e., total photon count per cell divided by cell size in pixels) is 26.86 (Fig. S11c).
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PMC11711347
While this study provides preliminary data from in vitro tests on MCF-7 cells, additional in vivo studies and eventual clinical trials are necessary to assess the effectiveness of the complex in breast cancer patients.
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PMC7802142
Using fluorescently labeled ReN-NV, we confirmed that their uptake by DPCs in a time-dependent manner when incubate them with cells.
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PMC10237474
Stable cell line generation via the widely established random genome integration approaches often takes months due to the necessity of single cell clone screening and selection.
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PMC8750422
Cells were seeded in 60 mm plates at a density of 2 × 10 cells and incubated overnight.
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PMC10940855
Corresponding clinical data suggest that that ephrin B2 expression correlates with adverse prognosis in patients with multiple myeloma.
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PMC11756907
2-ME has not entered Phase III clinical trials due to its low oral bioavailability, so research is ongoing to develop new derivatives and forms of the 2-ME drug.12–18 In recent years, graphene and its derivatives have become relatively accessible organic materials with well-defined structures with promising application opportunities in the context of targeted anti-tumour therapy.19–23 The cytotoxic properties of graphene oxides (GO) and reduced graphene oxides (rGO) have been demonstrated against a variety of malignancies, including melanoma.24–27 In these studies, GO and rGO are most often found to be the main component of a composite, on the surface of which there are nanoparticles or polymers, bonded by adhesive or covalently bonded forces, as well as immobilised substances with the properties of anti-cancer drugs, such as doxorubicin and cisplatin.
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PMC10606998
The therapeutic potential of SPIONs has been demonstrated in various preclinical models .
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PMC11718109
Since individual axons can be easily identified from these neurons by tracing their length, immunofluorescence of these cultures for VGLUT2 and synapsin demonstrated close colocalization of the two proteins in typical synaptic puncta (93.9% ± 7.6; mean ± S.D.), (Fig. 4A, A’, D), confirming presence of glutamate-containing SVs.
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PMC4839679
Phalloidin staining of F actin (red channel) and DAPI staining of nucleus (blue channel) in Caki2+Vectorand Caki2+PBRM1 cells.
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PMC11706297
NSCLC is commonly diagnosed at advanced stages, when surgical resection is inadequate in the majority of patients.
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PMC11519077
They found that although all MSCs display the same surface markers, the gene expression profile and secreted paracrine factors were different, leading to the higher potential for immunomodulation in MSCs from perinatal tissues versus the higher regenerative potential of BM-MSC.
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PMC11335267
F and G) Confocal microscopy images, taken at low (upper panels) or high (lower panels) magnification, of coronal sections of brains from mice expressing Agrp-tdTomato (F) or Pomc-GFP (G) and labeled with an Alx3 antibody.
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PMC9429973
Results: Collected data about availability of the different CML therapies shows substantial differences between countries in the region: 14 countries reported Imatinib available.
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PMC11459296
These models are invaluable for dissecting the molecular and cellular mechanisms underlying specific diseases and for driving the development of novel therapeutic strategies.
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PMC9622879
$$(1)-(18),(45).It is clear that the problem in (46) is an MILP problem that can be solved by many solvers.
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