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PMC11367939
As previously described , expression of the mRFP-conjugated Rab5 Q79L mutant in fibroblasts induced the formation of large endosomes positive for transferrin receptors that undergo endosomal recycling (Fig. 1D).
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PMC11740508
Non-linear curve fit, asterisks below red icons represent significant differences for M231 versus M231 cells, and asterisks beside green icons represent significant differences for M231 versus M231 cells.
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PMC11758416
See step 5 for example calculations.
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PMC11438317
This vector carries two homology arms and an in-frame STOP codon 12 bp downstream of the ATG start site of the gene and a blasticidin resistance cassette.
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PMC9429973
Within 28 days after HSCT, all pts became RUNX1 MRDneg, identifying this timepoint as least meaningful for MRD assessment in our cohort.
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PMC11049294
CytExpert Software 2.4 (Beckman Coulter Life Sciences, Brea, CA, USA) and Flowjo 9.6.1 software were used for analysis.
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PMC10317042
The development of new drugs for CCS is challenging due to the rarity of the disease.
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PMC11673128
Although CD47 is mainly upregulated by cancer cells, it is also expressed on many normal cells .
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PMC10604092
Each rat received an antibiotic (penicillin G potassium; Meiji Seika Pharma Co., Ltd., Tokyo, Japan) intraperitoneally before abdominal closure.
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PMC11576293
1H, SCH2CH = CH2, J = 1.5 and 9.9 Hz), 4.01 (2 overlap singlets, 6H, 2 OCH3), 3.82 (m, 2H, SCH2CH = CH2); C NMR (75 MHz, CDCl3) δ (ppm) = 171.7, 164.4, 163.3, 151.8, 149.3, 137.1, 135.4, 134, 129.5, 129.4, 129.3, 129.1, 128.9, 128.5, 120.4, 118.8, 117.5, 110.9, 110, 107.1, 56.1, 33.9; HRMS (ESI+) calcd.
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PMC9841531
These findings present compound 24 as one of the most effective MCT1 inhibitors with an optimized additional pharmacological profile, demonstrating its aptitude as an adjuvant therapeutic together with first-line antineoplastic agents in use to treat several types of cancers.
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PMC10376064
Additionally, ABCC1 is expressed ubiquitously in the human body, including the intestine .
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PMC11675244
Additionally, the lack of specific markers for non-canonical pathways obstructs the precise understanding of their role in embryonic and cancer cells.
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PMC11354239
The binding of the variable region of the therapeutic mAb to CD20 facilitates the binding of its Fc region to FcγRIII on NK cells, further leading to the formation of the immune synapse and triggering a response in cytotoxic NK cells to release granules containing perforin and granzyme B, which subsequently induce the cell death of the target cancer cell.
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PMC11347429
Functional pathway analysis using GO term enrichment for the up-regulated DEGs from the contrast of infected ICU patients versus healthy controls. (
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PMC10890055
On the contrary, the hFOB 1.19 cells withstood higher concentrations of ZnCl2 in the DMEM/Ham’s F-12 medium, which is recommended for this cell line.
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PMC11774112
Zeta potential of Co-PHRD at an N/P ratio of 10 determined by DLS. (
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PMC11513571
A p-value of less than 0.05 was considered significant.
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PMC10470467
First, we explore the difference in the expression level of each gene in tumor and normal tissue, as well as in the high- and low-risk group.
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PMC11791432
Visible colonies were fixed with 4 % paraformaldehyde, then stained with 0.1 % crystal violet for 15minutes, where colony-forming efficiency was evaluated by the number of colonies.
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PMC10900886
Alternatively, imDCs can become infected, and migrate to the lymphoid tissues, where they release newly produced viruses or spread virus to resident DCs or B cells.
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PMC11575040
According to previous studies [26–28], mitochondrial membrane potential (MMP) dissipation may result from severe energy deficiency, leading to cellular necrosis or even death.
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PMC9429973
DLBCL can be subdivided according to its cell of origin (COO) into germinal center B-cells (GCB), activated B-cell (ABC) and non-classifiable (NC) subtypes, with the ABC subtype having the worst prognosis.
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PMC11401350
The percentage of cells expressing CD69 activation marker is shown for CD8 TILs positive for the anti-CD4CAR (hCD34 +) or not (hCD34-) (mean (SD) n = 4, t-test,**p < 0.01).
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PMC10772747
T47D cells with a density of 2 × 10 cells/well were cultured in a 6-well microplate.
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PMC11476264
To investigate FBXO11 expression in the LUAD, we applied independent-sample t-tests to unpaired samples and paired t-tests to paired samples.
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PMC11291697
Ten µL of each sample were neutralised with NaOH and subjected to pre-column derivatization with o-phthalaldehyde and N-acetyl-L-cysteine.
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PMC11049294
As for future perspectives, the detection of GLA transcript variants, deep intronic variants and an aberrant methylation pattern via third-generation nanopore sequencing could also assist the molecular diagnosis, where a deficient enzymatic activity could be observed at single-cell resolution .
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PMC11632064
As our data indicate that OXPHOS is not associated with resistance, it would be interesting to determine which of the genes from our PLK1-targeting resistance signature are expressed in Rho-0 cells and whether this model can be used to generate resistant cells.
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PMC9596868
No. EI0001) and transferred onto Immun-Blot® PVDF membrane (Bio-Rad, USA, cat no. 1620177) using the Trans-Blot® SD semi-dry transfer cell (Bio-Rad, cat no. 1703940).
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PMC11583010
B, Internalization of chimeric 15A7.5 and 5A12.2 mAbs.
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PMC11453295
Since primary HMECs did not show robust ER expression, we also re-expressed ER using lentiviral transduction of ESR1 into HMECs.
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PMC9813422
Episomal maintenance of EBV and KSHV has been well studied.
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PMC11770199
Plasmids of PCDH-mCherry and PCDH-mCherry-p14 were transfected into cells.
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PMC11774112
The particle size of Co-PHRD at an N/P ratio of 10 was 164.1 ± 4.5 nm, and the zeta potential of Co-PHRD was 27.3 ± 1.38 mV. The flow cytometry and CLSM results revealed both DTIC and miRNA 34a were avidly taken up by A375 cells at 1 h and 4 h in PHRD.
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PMC9917080
The selenium-sorafenib nanocomplex in a wide range of concentrations induced the early stages of apoptosis in glioblastoma cells 24 h after application.
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PMC11489275
Mortality is still as high as 40–50% within 6 months of diagnosis and most deaths occur in the early stages of the disease .
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PMC9429973
We have tested 40 cDNA samples obtained from diverse RNA isolation routines, including 8 archival samples stored in the freezer for more than 6 months.
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PMC10578720
Taken together, these findings indicate that DCA treatment could partially rescue the impaired TCA cycle of macrophages in rAAV-1.3 HBV mice.
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PMC9429973
Type of donor was matched-related in 12 and matched-unrelated in 22 patients.
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PMC11782780
The results demonstrated that sh-EBF3 significantly promoted the sphere-forming ability of MDA-MB-468 cells, while the addition of 2-DG or 3-BP attenuated promoting effect conferred by sh-EBF3 on the sphere-forming ability of BC cells (Fig. 3F).
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PMC10850553
Tissue-type Plasminogen Activator as an up-stream factor to produce C5a from C5. (
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PMC11541409
Unlike traditional KIT/PDGFRA mutant targets, selinexor targets the XPO1-mediated nuclear export of RNA.
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PMC9429973
Here, we report a sensitive NGS assay to detect MRD in B-cell malignancies such as acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (CLL), multiple myeloma (MM) and mantle cell lymphoma (MCL).
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PMC11281366
To define the step of the infection cycle that is important for miRNA-26a expression downregulation, we treated cells with either the HIV-1 reverse transcriptase inhibitor efavirenz (EFV) or the HIV-1 integrase inhibitor raltegravir (RAL) prior to their infection with HIV-1.
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PMC11720808
The molecular docking result revealed that SCU directly interacts with TLR4 protein, with binding energy of −7.5 kcal/mol, which is similar to the binding energy between TLR4 and its inhibitor TAK242,30–32 suggesting that SCU binds firmly to TLR4.
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PMC11321678
ArchR offered addReproduciblePeakSet() function for peak calling by Macs2.
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PMC10887351
In specific, to investigate the β3-ARs’ involvement in PD-L1 expression and in the immune reactivity in the TME.
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PMC11658074
Molecular analysis, including qRT‒PCR and WB, revealed the upregulation of Col1A1 and the downregulation of MMP9 and CDKN1A in the fat graft group (Fig. 1i‒k).
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PMC9429973
Median (IQR) age at diagnosis and RBC transfusion initiation was 0.8 (0.4–2.8) and 1.3 (1.0–5.0) years, respectively.
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PMC11409031
The hdWGCNA method was employed to establish scale-free networks at the individual cellular level.
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PMC9429973
Data updates are needed to define the real incidence of DBS test positivity in MM.
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PMC11697065
Consistent viability profiles were observed across all pools apart from mAb1-expressing cells where viability sharply declined after day 9 (Fig. 1C).
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PMC11696576
However, the significant interactions between GK420 and amino acid residues were not observed in the docking simulations (Fig. S2 and Movie S1).
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PMC11095939
n.s.,
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PMC10938336
The human promyelocytic leukemia cell line was plated in 6-well plates at a density of 1.2 × 10 cells/well.
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PMC11394730
They further postulated that the unpredictability of this cascade could cause inconsistent RV effects.
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PMC9429973
Methods: This was an international open-label randomized phase III trial (NCT02172872).
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PMC9444294
D H&E, and H) negative IHC of case 27, which contains the alternative fusion site between SS18 exon 9 and SSX1 exon 7.
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PMC11481779
The encoder has one additional layer - the transformer encoder - that helps to learn the appropriate context of the latent representation produced by convolutions.
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PMC11742431
B, D, F) The 5637 cells, UMUC‐3 cells, and A375 cells treated with KVN and K14N were detected by Annexin V‐FITC/PI, respectively.
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PMC11596967
Upon scavenging ROS, antioxidants can transform into their oxidized forms, potentially exacerbating oxidative stress or causing DNA damage .
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PMC11615828
Furthermore, intracellular components can be mislocalized to the minus or plus end of microtubule networks within minutes by linking them with dynein or kinesin motors, respectively. , ,
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PMC11420784
n = 3 slides.
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PMC6803987
The results showed that HBeAg and HBsAg levels in the esculetin treatment groups were significantly lower than those in the model group.
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PMC11317131
IntePACE lagoon phage titers.
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PMC11476666
These findings indicate that stilbenes, and particularly DHS, affect stemness features of A549 cells and the SP fraction, suggesting their potential utility as anticancer agents, either alone or combined with chemotherapeutic drugs.
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PMC11627398
Meanwhile, the AUC of ROC curves for SLC31A1, MTF1, LIAS and LIPT1 genes were 0.854, 0.983, 0.774 and 0.936 in the GSE33118 cohort, respectively (Fig 2D).
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PMC11695623
The relationship between the expression of DCUN1D5 in GEPIA2 database and OS in patients with LUAD. (
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PMC11518702
Recently, a novel myeloid ortholog of HGAL, C1ORF150, has been described (19–26), which is conserved in Homo sapiens and primates but is not represented among mouse, rat, and lower vertebrate genomes.
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PMC9841531
The surprisingly high inhibitory power against ABCB1 prompted us to investigate whether the compounds (1.0, 2.0, 3.5, and 5.0 μM) were capable of sensitizing ABCB1-expressing A2780/ADR cells toward the antineoplastic agents 33.
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PMC10125624
However, amongst patients determined to be at high risk for thrombotic complications, those on tamoxifen therapy had a significantly higher risk of 90-day postoperative venous thromboembolism (OR: 1.15; 95%CI: 1.01-1.28; p=0.04).
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PMC5363531
The highest apoptotic effect was observed after sequential mD (32.4%; Figure 4A).
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PMC5173112
B, C) Colony formation assay in 786-O and A-704 cells transfected with transfected with retrovirus carrying empty vector or USP21-FLAG-HA.
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PMC11779605
A modified syngeneic mouse ID-8 Trp53 cell line (provided by Iain A. McNeish, University of Glasgow) was used.
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PMC8873393
Meanwhile, CSB recruits the PAF1 complex onto RNAP II paused at TSS sites to promote pause release and stimulate productive elongation throughout genes .
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PMC11306019
Therefore, our study aims to unravel the structure-activity relationship of dietary polyphenols and their BTZ antagonism at daily diet-achievable physiological concentrations.
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PMC9429973
Furthermore, there are some concerns in terms of morbidity and mortality for patients with FL because of their immunocompromised status induced by recent exposure to cytotoxic chemotherapy, especially bendamustine and anti-CD20.
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PMC11622247
These probes contained the second auxiliary dye in 10 or 7 nt distance to the first AF568 dye.
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PMC11634027
Bottom two panels show blots on proteins immunoprecipitated with anti-FLAG beads (J) and anti-V5 beads (K) and detected with V5 or FLAG antibodies. (
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PMC9429973
Induction of remission was carried out by three-component regimens including bortezomib, 30 patients required two or more lines of therapy with lenalidomide or daratumumab.
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PMC9429973
Aims: To estimate the per-patient lifetime direct healthcare cost of TDT from a US healthcare payer perspective using an economic model developed based on published model frameworks.
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PMC10366908
The activation of the EMT program confers tumor cells the ability of migration, invasion, extravasation, and stemness .
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PMC10656496
Raw sequence data were demultiplexed using the Illumina bcl2fastq2 software (Illumina, Inc., San Diego, CA, USA).
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PMC11518994
C PAX5 variant allele analysis.
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PMC10758402
Dimethyl fumarate (DMF) (Sigma-Aldrich) was resuspended in 100% DMSO to 100mM and further diluted at indicated dilutions before use in all in-vitro experiments.
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PMC11319300
Interactions where MYEOV promoter was taken as bait are highlighted in green.
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PMC9429973
The molecular study by NGS of the BM samples was performed using MYS (Myeloid SolutionTM) from SOPHiA Genetics.
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PMC10706275
EGFP gene expression in SSI cell lines was evaluated through flow cytometry.
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PMC10936243
As we are using the metal cages, if we use bedding, we are afraid that it may contaminate, especially for the male cats… we don’t put it in as it may cause infection at the place of the surgery” [D081]. “…
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PMC11578343
D d is related to D cell by conservation of volume: (3) Dd=2Dcell33wcontraction The stress on the cell can then be determined using Equation (1).
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PMC11087766
PhiKZ PicA-sfGFP expressed in an uninfected P. aeruginosa PA01 K2733 cell (Left) or Goslar PicA-sfGFP expressed in an uninfected E. coli MC1000 cell (Right). (
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PMC11773391
O‐methylguanine‐DNA methyltransferase (MGMT) is the most crucial mediator for repairing DNA damage caused by TMZ.
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PMC9847912
Further studies of HIV-2 maturation might lead to compounds with a broader spectrum of antiretroviral coverage, including those with activity against HIV-2.
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PMC9429973
Therefore, strategies to prevent relapse are urgently required.
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PMC7961460
Thus, the increment in HA synthesis by stromal cells may be the consequence of paracrine signalling from tumour to stroma .
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PMC10729469
Post-stimulation, cells were stained with fluorescently labeled Aβ-tetramer (MHCII-IA–KLVFFAEDVG-SNKGA) to evaluate the frequency of TCRAβ-reactive CD4+ CD25+ Tregs.
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PMC11473843
GRCh38.104).
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PMC11767817
In previous works, we determined the IC50 values of curcumin and its derivatives for the human bladder cancer cell lines 5637 and SCaBER under normoxic conditions .
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PMC11395280
To determine if CCL198-83 could be used to inhibit the migration of CCRF-CEM into the CNS, we used the BBB model (Figure 3).
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