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PMC8992508
The best quinoxaline derivatives (8-13), displayed IC50 values in the range 1.4-2.2 μM that are lower than those of the 2-naphthyl or 3,4-methylenedioxyphenyl analogues (compounds 14-18 and 19-22, respectively).
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PMC11802855
Further analysis revealed 16 distinct clonal subpopulations of MM cells (Fig. 2E).
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PMC11544771
Here, we show that CPX is synergistic with PRE in inhibiting cell proliferation and inducing apoptosis of NSCLC (A549 and A427) cells.
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PMC9429973
Methods: An unanchored matching-adjusted indirect comparison was conducted using individual patient-level data (IPD) from 150 patients treated with tec 1.5 mg/kg weekly in MajesTEC-1 (clinical cutoff of Sep 7, 2021), and published summary-level data from 97 patients treated with the approved dose of belamaf (2.5 mg/kg every 3 weeks) in DREAMM-2.
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PMC9429973
S. Harbi, J.-M. Boher, E. Forcade, P. Chevallier, R. Peffault De Latour, F. Malard, S. Francois, A. Charbonnier, E. Hermet, C. E. Bulabois, A. Huynh, A. Berceanu, T. Cluzeau, M. T. Rubio, S. Furst, R. devillier, M. Mohty, D. Blaise hematology; DRCI, Institut Paoli Calmettes, Marseille; hematology, CHU, Bordeaux; hematology, CHU, Nantes; Transplant Unit, Hopital Saint Louis; hematology, Hopital Saint Antoine, Paris; hematology, CHU, Angers; hematology, CHU, Amiens; hematolgy, CHU, Clermont Ferrand; hematolgy, CHU, Grenoble; hematology, CHU, Toulouse; hematology, CHU, besancon; hematology, CHU, Nice; hematology, CHU, Nancy; hematolgy, Institut Paoli Calmettes, Marseille, France Background: In most situations where allo HSCT is wished a matched sibling donor (MSD) is lacking.
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PMC11678841
Pre-treatment with 25 µM GA significantly increased P-ERK1/2 expression in infected VECs (Figure 4A,B).
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PMC8806312
Heatmap of 22 immune-infiltrating cell types in tumor samplesFigure 8.The level of immune cells and Kaplan-Meier analysis in patients with OS. (
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PMC11724582
The YAP/TAZ expression level in EGFR wild-type A549 cells was lower than that in EGFR T790M mutant H1975 cells, which are more sensitive to almonertinib (Fig. 5A).
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PMC11013014
Apparently, the secondary amine is more reactive than the primary amine.
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PMC9878278
SC treated with poly (I:C) in the presence and absence of BMP6 and mRNA expression of (E) IFNB1, and (G) IFIT1, and MXA was measured by qRT-PCR. (
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PMC9780037
In fusion protein-based delivery systems, cyt c (represented in blue) is connected to its conjugated protein or peptide via a linker containing a disulfide bond.
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PMC10873328
GPC1, as well as glypican-1, is specifically enriched on cancer-cell-derived exosomes and may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer.
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PMC11543885
Seed 1 ×10 HEK293T cells per well on the PLL-pre-coated 96-well plate in 100 μL complete medium and incubate the cells for 16 h at 37°C with 5% CO2.
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PMC11697189
Cells were seeded into 6-well culture plates and allowed to reach approximately 100% confluence before the monolayer was scratched using a sterile p200 pipette tip.
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PMC10565698
B. Expression of circUSP10 in chromosomes and its composition.
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PMC6901583
Scale bar: 100 μm; **P < 0.01; ***P < 0.001.
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PMC11599565
Hence, EMT-MET-linked cell plasticity is not only confined to the ability to acquire chemoresistance or migration/invasiveness capacity, but it also involves the ability to activate dedifferentiation programs through which one type of cell differentiates into another, mainly orchestrated by environmental determinants. “
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PMC11388384
Cytonemes were ablated in two independent Myo10 clonal lines (denoted −786 and −791), and augmented in cell lines expressing the artificial transport vehicle tethering Espin to the tip of cytonemes (ATV(+)/Espin).
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PMC11411131
The TetR DNA binding domain interacts with the Tet response element (Tre), with Krab functioning as a transcriptional repressor.
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PMC11629461
UVA irradiation of the NOX5‐CHO‐K1 cells was from a LED 380 nm (width at half height 10 nm) source (LAMPLIC, Shenzhen, China, http://www.szlamplic.com/), doses (380 nm, 0.1, 0.3, 3.5, 6, 12 mW/cm, 1.5 min) started from 0.1 mW/cm, based on previous work in RBL‐2H3 (RRID: CVCL‐0591) cells (380 nm, 80 μW/cm, 1.5 min).
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PMC11543853
We speculate that the newly assembled virions do not release their genomes because they have not been stimulated by environmental factors, including receptor binding, acidic pH, and an ion composition distinct from that of the cytoplasm.
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PMC4685341
The absence of selective pressure resulted in the lowest GntIII mRNA levels and thus the lowest non-fucose levels.
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PMC10073922
Fluorescence microscopy was performed on an inverted microscope (Hund, Germany) with filter sets designed for GFP.
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PMC11453018
The analysis was performed using the default settings of the HSA model, which compares the efficacy of combination treatments relative to the most effective single-agent treatment in each condition.
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PMC11635519
MCP1-GFP peptide and CCR2-mCherry were localized at the cell surface when expressed separately. (
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PMC9583612
Methane mitigation from the oil and gas (O&G) sector represents a key near-term global climate action opportunity.
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PMC8708099
Hoffman et al. demonstrated a positive correlation between IL-8 and NFκB, which was also found in our study after Caco-2 incubation with histamine (Figure 4A), and may explain the inhibitory effect of osthole on IL-8 secretion .
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PMC9220170
SELENOM-KD and SELENOT-KD lead to impaired activity and Ca capacity in the ER.
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PMC11066331
Frozen cell pellets were thawed on ice and diluted with ice-cold 150 mM ABC buffer.
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PMC11737091
Apoptosis of 786-O and A498 cells increased significantly after DBF4 expression was knocked down. (
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PMC6684588
Photomicrographs of representative cross-sections through ACE-083-injected, vehicle-injected, and uninjected (contralateral) TA muscles in which an antibody directed against laminin was used to delineate individual fibers.
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PMC8140214
Trypsin was from BDH, England.
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PMC11777207
Statistical significance was determined using Student’s paired t test. *
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PMC11656048
The detailed measurement was conducted according to the kit instructions.
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PMC11591794
Although the war against cancer worldwide has been fought for years, its morbidity and mortality have not decreased.
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PMC9844987
We profiled GM12878 TSSs and promoter activities with CAGE before and after 6 hr treatment with TNFα (Supplementary file 4).
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PMC7961460
Indeed, we found that cancer cell line CM is a source of an uncharacterized protein that can influence HAS2 expression.
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PMC9429973
Median (range) baseline mIDH1 variant allele frequency in BMMCs was 36.7% (3.1–50.5%) in the IVO+AZA arm and 35.5% (3.0–48.6%) in the placebo+AZA arm.
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PMC3274243
The high impedance measurement system results in a negligible current flow within the system of less than 10 amps cm.
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PMC11240571
Recently, combination therapy of RT and anti-PD-1/PD-L1 antibodies has attracted significant attention.
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PMC9429973
Aims: The aim of the study was to study baseline clinicohematological characteristics, and cytogenetics profile of this disorder in our population Methods: A cross sectional study was conducted at National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi Pakistan from 2018-2021.
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PMC11772449
Control small interfering RNA si-NC and si-p38γ were transfected into hepatocellular carcinoma cell lines BEL-7404 and Huh-7, respectively.
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PMC11334037
The cells were cultured for 2 d at 37 °C with 5% CO2.
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PMC9000591
The following samples including SE3 and SE4 had IC50 values of 159.82 and 151.69 µg/mL, respectively.
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PMC7582629
Therefore, the degree of blue shift can also be treated as a Raman signature to discriminate cancer cells from normal.
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PMC10988555
SI values calculated considering that the IC50 would be 100 μM (assuming the minimal value); – SI values not calculated.
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PMC10440586
The CD99 expression of bevacizumab-treated mice versus dibenzazepine-treated mice and control group. (
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PMC11237030
These treatments provide short-term relief and require a continual use to reach maximal benefits.
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PMC11711127
This anergic T cell development in the lymph nodes draining the tumour correlated with heightened TCR signalling but reduced proliferation and functional activity of effector cells.
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PMC9220170
Thus, SELENOM in different tissues can regulate the activity of two antioxidant enzymes in different ways.
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PMC9429973
Whether prophylactic use of emerging agents, such as azacitidine, can reduce post-transplant relapse rates remains to be further explored in prospective studies.
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PMC11046454
Tumor microenvironment-responsive CAR-T system. (
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PMC9429973
An additional 14.2% received other treatments or had missing treatment information and were excluded from further analyses.
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PMC10849234
Cells were then left undisturbed for 6–7 days at 37°C in 5% CO.
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PMC7723249
Statistical analysis: student t-test (*: p<0.05; **: p<0.01).
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PMC11363012
Additionally, the diameter of each cell was measured from the images acquired during the cell picking, allowing us to estimate the volume of the cell, which was then used to calculate the concentration of each of these molecules in an individual cell (Supporting Information Data 4).
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PMC11763111
293 T cells were maintained with Dulbecco's Modified Eagle Medium (Gibco, 11995065), supplemented with 10% FBS and 1% penicillin–streptomycin solution.
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PMC11746948
ns not significant.
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PMC11774747
The reported risk of post-administration GvHD is relatively low, around 10%, regardless of the antiviral VST type and donors, including third parties with partial HLA compatibility (70).
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PMC7334607
Instead, the evolution of resistance to one drug may lead to variable changes in response to the second drug.
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PMC11696576
In line with this hypothesis, we found a significant overlap between the genes upregulated by AVX420 at 16 h and genes upregulated by the knockdown of BMI1, which is an important component of PRC1 and related to the activity of PRC2 (Fig. 7b).
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PMC8864075
In short, reTFAE interferes with the sugar metabolism of A549 cells.
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PMC11657695
Target gene expression was normalised to HPRT, B2M or ActinB levels in respective samples, and the Cycle threshold (Ct) method was used to measure the relative quantification of target mRNA.
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PMC9878278
The cells were infected at 48 hpt and virus replication was measured.
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PMC8270637
Comparisons between two and multiple groups were performed using Student’s t-test and one-way analysis of variance, respectively.
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PMC11670407
c KEGG pathway enrichment analysis of 496 proteins that interact with OTULIN.
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PMC9429973
Majority of patients were analysed before 1L therapy (n=61; 95,3%), and had following clinical stages: Ann Arbor stage I 23.4%, stage II 37.5%, stage III 15.6% and stage IV 23.4%.
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PMC11684821
There were some differences between the 2 clones, as MC.27.759S was better at killing K-562 than MC.7.G5 (96.1 % versus 72.9%), and MC.7.G5 seemed to prefer SiHa target cells (84.9% versus 40.3%) (Figure 2A).
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PMC11036064
.
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PMC11486946
g Dose-dependency of gefitinib on MSD.
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PMC10605143
Nevertheless, during development (e.g., embryogenesis), epithelial cells may give rise to mesodermal tissue (among others, mesenchyme), which involves the profound modification of epithelial cell phenotypes.
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PMC11306019
Collectively, our findings indicated that the structural configuration, origin, and number of VDMs are crucial in determining the extent to which polyphenols counteract BTZ.
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PMC10669966
Consistent with the transcriptome data, elevated PDE3A expression was associated with the MLPS subtype, but also with male gender (Table 3) (p < 0.05).
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PMC9429973
On behalf of the NCRI AML Working Group, Manchester, United Kingdom Background: Liposomal daunorubicin and cytarabine (CPX-351) has shown a survival advantage for older patients (>60years) with secondary AML compared to 3 + 7 chemotherapy however in younger patients there is a lack of randomised evidence for benefit.
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PMC9429973
Figure 1.
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PMC9429973
We constructed a case-matched cohort matched on age, sex, AML subtype and BMI (figure panel B+D) and performed a sensitivity analysis using ≤90% cut-off to define DR which led to the same conclusions.
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PMC11585994
The prepared nanoparticles were diluted in pure water to achieve a concentration of 1 mg/mL. The resulting nanoparticle solution was then applied to a copper grid for transmission electron microscopy (TEM) imaging and allowed to air dry at room temperature.
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PMC11742290
LGR5 protein (100kDa) is conserved across humans, rats, and mice, with all homologs consisting of 907 amino acids.
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PMC11594641
The complexes were added to cells and cultured for 2–3 days at 37 °C.
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PMC11172027
Therefore, in this work, we also collected data from several NALM concentrations on another type of cancer cells, namely leukemia cells, in the form of the Jurkat cell line.
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PMC10507284
The flow cytometric analysis results of the cell cycle for SW982 cells in NC and TROAP‐siRNA groups (n = 3 for each group).
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PMC8316344
TRIB2 is a pseudokinase that has a major function to act as a molecular adaptor mediating protein ubiquitination and followed destabilization via E3s .
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PMC9429973
ERN-EuroBloodNet launched the collaborative platform on patients with red blood cells and COVID-19 containing so far 373 patients.
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PMC10317042
Data in D, means ± SDs. *,
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PMC9684669
To further identify compounds that showed combination effects with doxorubicin, these 246 compounds were evaluated at 11 concentrations in combination with four doxorubicin concentrations at 0.1, 1, 10, and 25 µM, separately.
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PMC11449273
In this model, we postulate that in the absence of plakoglobin, mutant p53 (175 and 273) and β-catenin translocate into the nucleus and interact with oncogenic transcription factors to activate gene expression associated with tumorigenesis.
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PMC11532793
Our analyses included edgeR with count scaling under both v3 and v4 pipelines as well as other previously benchmarked DTE methods reported in (13).
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PMC11752740
This study highlights the potential anticancer effects of pistachio hull extract in combination with CFZ.
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PMC9429973
Long-term glucocorticoid therapy and rituximab therapy could probably activate hepatitis virus replication and aggravate the disease.
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PMC11742670
This clarifies how the CNN structure takes out features from input data and conducts regression using many pooling and convolutional layers, along with fully connected layers.
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PMC9429973
Median nadir neutrophil count, evaluated for every patients for at least three courses of therapy (r. 3-6) registered at day +11, was 1.28 (r.0.9-2.2).
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PMC10976516
Based on histological characteristics, NSCLC is further categorized into lung adenocarcinomas (40%), squamous cell carcinoma (25%–30%), giant cell carcinoma (10%–15%), and some not otherwise specified (15%–20%) (Figure 1).
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PMC11738017
PFS of patients treated with teclistamab at recommended phase 2 (RP2D) based on sBCMA cutoff of 400 ng/mL. (F) PFS of patients treated with teclistamab RP2D based on the sBCMA median cutoff.
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PMC9429973
Patients were included if they were ≥ 18 years and had localized pulmonary involvement.
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PMC11139449
This experiment was repeated three times with six replicates for each repetition to ensure the accuracy and reliability of the results ( 16 ).
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PMC11473843
Blinding in mouse studies was not possible due to tagging of the mice and access to identification codes by the investigators.
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PMC11342785
When the PKA substrate is phosphorylated in response to cell-signaling inputs, TPPP will be connected to the IDR component to stimulate synMAP circuit activity.
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PMC11770199
Cell membrane was labeled with 5 μg/ml CellMask (Invitrogen, USA) at 37 °C for 10 min.
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PMC11740399
These data also suggested that the level of PKM2 was regulated by the PI3K/AKT/mTOR axis.
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PMC9429973
This study was approved by the Ethics Committee of the Chinese PLA General Hospital, and signed informed consents were obtained from all patients prior to treatment in accordance with principles of Declaration of Helsinki.
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