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PMC11612794
Secondary antibodies used in immunoblot analysis were horseradish peroxidase-conjugated (HRP)-conjugated goat polyclonal anti-rabbit IgG, HRP-conjugated goat polyclonal anti-Rat IgG, and HRP-conjugated rabbit polyclonal anti-mouse IgG (Jackson ImmunoResearch).
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PMC9429973
Since frontline protocol in MM changed recently, induction therapy in group A and B was, respectively, VCD 60 vs 26%; VRD 30 vs 75%; VTD 10 vs 0% (P<0.001).
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PMC10452486
Furthermore, the glycosylation pattern of Face-2 may differ from that of Face-1.
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PMC10878518
Their titers were determined by a plaque-forming assay using 293 cells, and they were stored at –80°C.
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PMC9429973
Human NK cells are the first lymphocyte recovering after allo-HSCT.
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PMC10440586
At both transcriptomic and proteomic levels, compared to the NP group there was significant overexpression of CD99 in the RP group with short-term recurrence (Figs. 4A and 4B).
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PMC9964635
CNMR (126 MHz, DMSO), δ 165.75, 158.10, 149.03, 139.60, 139.33, 138.68, 129.25, 128.74, 126.77, 123.42, 119.04, 44.10, 37.79, 35.41, 29.88, 29.49, 22.14, 20.89 (Figures S21–S23).
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PMC11413393
For PRMT1 knock down lines, all samples had RIN values = 10.0 and libraries were prepared with the Illumina mRNA kit.
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PMC9429973
In time, more data will become available for safety and effectiveness of RAV under real-world conditions.
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PMC5833712
To further investigate this effect of ERβ on ERα protein stability, the A2780cis/ERα cells were treated with KB9520 in the absence or presence of the proteasome inhibitor MG132 (Fig. 3b).
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PMC11407042
Note: A seeding density of 3 × 10–1×10 cells/cm is recommended.
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PMC11185260
To visualize and measure intracranial tumors, animals were imaged in vivo without the aid of a contrast agent on a 7T Bruker horizontal spectrometer/imager run using Paravision 5.1 software (Bruker Biospin MRI GmbH, Karlsruhe, Germany).
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PMC10969097
In the study by Zhang et al. , the results initially showed that the percentage of dead cells was lower when PC3 cells were cultured with conditioned medium derived from CAFs, suggesting that some components of this medium protected the cells from docetaxel-induced death.
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PMC10887351
β-ARs were over-expressed in BC in relation to non-diseased breast epithelium .
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PMC11711663
Nomogram from the fitted Cox model.
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PMC11025866
The effects may involve functions at the level of RNA and/or protein expression, protein localization and activity, and downstream changes in metabolism and cell signaling.
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PMC9065483
To combat this, small molecules that transiently modify the interferon response have been investigated in order to recover oncolytic efficacy.11, 12, 13, 14 Our group has identified sodium orthovanadate (vanadate) and other vanadium-based compounds as being capable not only of attenuating the antiviral IFN-1 response, but also simultaneously increasing proinflammatory activity through type II interferon (IFN-2) signals when co-administrated with the oncolytic vesicular stomatitis virus (VSVΔ51).
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PMC11310713
After menopause or decreased ovarian functions caused by primary ovarian insufficiency and related diseases, most of the side effects are from hormone deficiency produced by gonads, leading to disruption of the HPO axis system .
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PMC9429973
Aims: We examined genome-wide DNA methylation patterns in BMSCs derived from either femoral head bone marrow or bone marrow aspirates of the iliac crest of healthy donors to identify the optimal control cell population for hematological malignancy research.
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PMC11803918
For the analysis involving two or more variables, two-way ANOVA (Kaplan–Meier log-rank test) was applied.
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PMC8358006
Lentivirus was produced using HEK293T cells with the second-generation packaging system psPAX2 (#12260, Addgene) and pMD2.G plasmid (#12259, Addgene).
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PMC11206251
The supernatant was discarded, and 500 μL of 75% ethanol was added and centrifuged at 4 °C at 10,000 rpm for 5 min.
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PMC11049294
Also, a gene therapeutical approach is under development, currently being in clinical trial Phase I/II (NCT02800070).
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PMC9878562
We also sorted DOHH2 cells for ACKR3 surface expression and measured the transcriptomes.
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PMC11267830
c The proportions of apoptotic DCAF15 and DCAF15 cells after drug treatment were measured by flow cytometry.
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PMC11564405
B Anticancer immunity score across a seven-step cancer-immunity cycle in the low RILPL2 expression group and high RILPL2 expression group Relationships between RILPL2 expression and anticancer immunity scores based on the TIP database.
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PMC9429973
Image: Summary/Conclusion: FeO is a common complication affecting 41% of pts with PK deficiency in this cohort, regardless of age, genotype, transfusion status, or splenectomy status.
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PMC11487720
Black arrows indicated bubbles.
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PMC10969097
The decrease in viability was mediated by apoptosis and necrosis.
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PMC11286266
Assuming the endoplasmic reticulum (ER) constitutes 10% of cell total volume (Alberts et al., 2002), the concentration of CRT in the ER of CHO-K1 cells was estimated to be around 7 µM. Top panel: Immunoblot showing CRT and actin levels for parental XC45-6S cells and known amounts of purified bacterially expressed rCRT.
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PMC7433824
Of the 12 common proteins, 11 proteins were upregulated; only mitochondrial 60 kDa heat shock protein was downregulated (P < .05).
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PMC9429973
The primary endpoint was investigator-assessed PFS.
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PMC11684297
The pH of the solution was made alkaline (pH = 12.5), and the solution was refluxed at 90 °C for 2 h. After the reflux was completed, a white-colored precipitate was observed in the walls of the refluxing flask, which was then scrapped off using a spatula and collected in a tube.
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PMC11040965
We cultured BMMC (5 × 10/well) in 96-well round-bottom plates (BD Biosciences) in 200 µL/well TexMACS medium (Miltenyi Biotec) containing 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin.
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PMC4395774
Mouse primary CD8 T cells were isolated from spleens of prf mice using CD8a (Ly-2) microbeads (Miltenyi Biotec) following the manufacturer's instructions.
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PMC9429973
Supplementary oxygen was used in 81.6%, in hospital-treatment was necessary in 84.1%, and 41.4% required ICU admission.
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PMC9429973
Referrals were made with a provisional HSCT date in 83% of cases.
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PMC9429973
From the patients treated with plasmapheresis, 19 (73%) started the treatment on the day of admission or first day of hospitalization.
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PMC11435360
In comparison with the BBN saline group, monotherapy with PAG and GEM attenuated tumor growth (p < 0.002 and p < 0.0002, respectively) and further attenuated with PAG and GEM combination therapy (p < 0.0001, Figure 3A).
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PMC11036064
Using a StepOnePlusTM Real-Time PCR System (Thermo Fisher, MA, USA), the mRNA expression of CASP9, CASP3, CASP8, MMP2, CD44, CDK6, CDK4, CCND1, RAF1, MAP2K1, MET, SRC, and CD274 was studied.
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PMC11727638
After forming this core gel at 5 °C for 15 min, PBS (10 µL) containing 0.5% w/v alg-Ph, 1.0% w/v gela-Ph, and 1 mM hydrogen peroxide (H2O2; Fujifilm Wako Chemical) was dropped on the core gels to form hydrogel shells through HRP-mediated hydrogelation.
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PMC11352877
The elevated expression of VEGF-A has been proven to contribute to a more aggressive phenotype of the disease .
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PMC11718109
Moreover, three morphologically distinct populations of such clusters were observed.
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PMC10551595
Then cell viability was determined using the MTT assay.
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PMC11387401
Hence, the RNA interactors found for the cleaved MA and NC proteins should be interpreted in this context.
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PMC11319300
MYEOV has been associated with the regulation of microRNAs miR-17-5p and miR-93-5p, possibly by interacting with MYC (Shen et al., 2021).
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PMC9429973
RE-MIND2 (NCT04697160), an observational, retrospective cohort study compared patient outcomes from L-MIND with matched patient cohorts treated with other NCCN/ESMO recommended therapies.
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PMC3035438
The frequency of sequence alterations was comparable to the frequency usually observed in TSA-treated wild-type DT40 (5,7).
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PMC9429973
Summary/Conclusion: Patients with MCL previously treated with cBTKi experience very poor outcomes, as measured by their duration of subsequent therapy and OS.
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PMC11306331
Indeed, we observed robust dissolution of illuminated single droplets with only minimal effects on droplets in distal regions of the nucleus (Fig. 2I; Fig. S3D; Supplementary Movie 3).
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PMC10900886
Enterovirus D68 (EV-D68) is a small non-enveloped, positive single-stranded RNA virus that belongs to the family Picornaviridae, genus Enterovirus.
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PMC9817179
The change in standard deviation between the two images is –48%.
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PMC6461034
In a treatment setting, INKA analysis clearly revealed a specific drug effect after targeting EGFR in U87‐EGFRvIII, with the high, first‐rank INKA score for EGFR at baseline being halved after treatment with erlotinib (Fig 5B).
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PMC11594806
As discussed, nanoparticles like pegylated liposomal doxorubicin and liposomal mifamurtide have shown effectiveness in treating sarcomas.
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PMC11224020
To gain insights into the mechanisms accounting for this specificity, we compared the transcription factor binding sites found in the promoters of the genes enriched in DCs confined at a height of 3 µm or treated with LPS.
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PMC11011837
Similarly, the separation of the cortical cell line, Molt-4, and ultimately the separation at the first component level of the two mature T-type cell lines, Jurkat and Molt-16, from the other two can be explained.
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PMC10813895
As we age, ELF5 decreases, possibly increasing cancer risk.
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PMC11078693
Work in a dimmed light environment for the remaining steps.
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PMC11727000
A maximum of two missed cleavages was set in the search using trypsin as the enzyme in a specific mode.
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PMC10944868
On the other hand, only four of the compounds studied showed cytotoxic activity against the A549 line, where compound 12E obtained the best result with an IC50 value of 9.89 ± 0.25 μM (Table 1, entry 24).
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PMC11754784
The pre-stained CEM cells were washed three times with washing buffer, and mixed with K562, Ramos and RPMI-8226 (1 × 10, each cell line) cell mixtures in binding buffer.
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PMC11656380
Cytotoxicity of the Tat-Ram13 is correlated with the macropinocytic activity of the T-ALL cells. (
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PMC10840195
Representative images and statistical analysis (B).
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PMC7351993
e Procedure for sorting Euglena gracilis cells.
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PMC10933092
A, B) The best parameter (λ) is selected based on the least absolute shrinkage and selection operator (Lasso) model. (
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PMC10772747
The cells were maintained at 37°C with 5% CO2 in a humidified atmosphere.
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PMC11806106
Measurements of cells stably expressing the MOR-FRET-sensor were performed in a plate reader (Spark 20 M, Tecan).
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PMC9429973
Extra hematologic features among index cases: 49 patients (55%) had at least one clinical feature of dyskeratosis congenita.
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PMC7762347
The gene expression analysis was evaluated only after 72 h exposure of cells to CYN (0.5 µg/mL), MC-LR (1 µg/mL) and CYN/MCLR combination.
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PMC11705901
Second, it is possible that these SNPs are actually not functional but are in linkage with other SNPs that were not assayed in this study and were not measured in previous studies of human traits.
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PMC10988808
As ITGA3 is a crucial protein regulating tumor EMT (epithelial-mesenchymal transition), we further confirmed that overexpression of CCDC25 suppresses EMT in ccRCC cells (Fig. 3E, F).
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PMC9429973
Despite its high sensitivity and specificity, the prognostic informativeness of MRD monitoring through RT-qPCR varies widely among different genetic subgroups.
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PMC11381192
Clinical characteristics, including age, sex, bone marrow plasma cells (BMPC, %), lactate dehydrogenase (LDH, U/L), albumin (ALB, g/dL), β2‐microglobulin (BMG, mg/L), haemoglobin (g/dL), creatinine (mg/dL), cytogenetic abnormalities, International Staging System (ISS) stage and survival status, were collected and compared between high and low CSI patients.
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PMC11696576
The inhibitors, or DMSO vehicle control, were subsequently added to the cells for 1 hour prior to stimulation with 10 ng/mL IL-1β for 4 h. Supernatants were collected and centrifuged at 300 × g for 5 min to clear them of detached cells and adherent cells were dissolved in 1 N NaOH.
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PMC11790599
Briefly, cells were seeded at 2–4 × 10 cells/well in 6-well-plate overnight.
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PMC9119314
The plates were placed into a modular incubator; gassed with 93% nitrogen, 3% oxygen, and 4% carbon dioxide; and incubated at 37°C for 48 h. The parasite growth was assessed by measuring lactate dehydrogenase activity as described by Makler et al. (1993).
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PMC3681794
However phosphorylation of p53 protein on serine 15, an indication of p53 activation, was only detected after exposure to cytotoxic drugs (Figure 8A).
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PMC10812665
Each time point had its own DMSO control.
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PMC9454852
The major downstream pro-apoptotic factor is CHOP, which in physiological condition shows a low expression level that is enhanced under ER stress.
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PMC9479186
The number of invaded cells were observed and counted in three randomly selected high-power fields under light microscope (×200).
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PMC9429973
The median age was 64 years (35-88 years).
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PMC8632470
N-Acetyl-p-benzoquinone imine (NAPQI) is a reactive metabolite of acetaminophen (paracetamol), which has been confirmed for its damage effect on the liver and kidney due to glutathione depletion.
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PMC11244823
All 110 serum samples, including the validation sera No. 29, No. 62, posC1 and posC2 were tested in the EHV-1/EHV-4 Ab ELISA kit from Svanovir (Svanova, Uppsala, Sweden) .
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PMC11549062
Schematic diagram of the relationship between PTP1B and PDGFR‐β on endocytosis system.
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PMC11772585
The ratio of JC-1 red/green fluorescence can serve as an index for MMP and can also reflect early cell apoptosis.
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PMC9429973
Cox proportional hazard regression was applied to investigate the independent prognostic factors of OS and CSS.
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PMC11473750
Methionine 158 is located in transmembrane helix 4.
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PMC11082662
SMPDL3B, sphingomyelin phosphodiesterase acid-like 3B; TCGA-OV, The Cancer Genome Atlas-Ovarian Cancer; OS, overall survival; PFS, progression-free survival; HR, hazard ratio; CI, confidence interval.
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PMC8540692
They found that curcumin in HeLa and SiHa cells and resveratrol in Caski cells caused significant reactivation of PAX1 expression; however, the reversal of promoter hypermethylation was not observed across the three cell lines.
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PMC11726183
The simulation was carried out using the particle mesh Ewald (PME) method for electrostatics at 9 Å cut-off using an isobaric–isothermal ensemble (NPT) with 300 K temperature and 1 bar pressure for 100 ns.
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PMC11487720
Endogenous NO production was then measured by using a live cell dye, DAF-FM , for time-lapse microscopy at 22 °C (Fig. 3A, B).
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PMC11748335
We thus analyzed H3K4me3 ChIP-seq data from 109 Roadmap samples to identify CGI promoters in each sample that exhibit concurrent H3K4me3 and H3K27me3 modifications.
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PMC9822769
Several transporter proteins have been associated with cisplatin influx (with a variable level of evidence and positive or negative effects on overall chemotherapeutic efficiency): copper transporters, especially Ctr1 (SLC31A1), and, to a lesser extent, organic cation transporters, OCTs [29, 33–36].
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PMC9429973
In patients with vaccine associated ITP, using TPO agonists earlier might be considered to avoid the use of immunosuppressants shortly after vaccination, which might impair the intended immunity against SARS-CoV-2.
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PMC11190238
c Heatmap representation of PC1 and expression values of epithelial (right) and mesenchymal (left) genes in TRO (EMT score = −0.17) and MSC (EMT score = 0.62).
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PMC2196094
The obtained sequence covers the central region between the His and Ser residues of the catalytic triad (Figs. 2 B and 5 B). (
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PMC11789597
Thus, the expression of SLC1A5, SLC7A5, and SLC3A2, which are related to glutamine and amino acid transport and regulated by CAV1, was further analyzed.
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PMC11705862
Western blotting assessments of separate representative repeats for Cdk5, total tau, and phosphorylated tau (AT8 antibody targeting phosphorylation at Ser 202, Thr 205) protein levels, respectively, in SH-SY5Y cells with and without CD59, IRIS-1, and IRIS-2 knockdown.
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PMC11461874
The exon 4 is skipped in TRAF7 KO cells by using CRISPR-Cas9 genome editing.
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PMC9164404
However, Abplatin was believed to be reduced by intracellular reducing agents such as glutathione(GSH) and ascorbic acid to release Pt(II) for DNA binding.
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