PMCID
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PMC9429973
Aims: Our objectives were to investigate the immune micro-environment of bone marrow (BM) trephine biopsies from patients with high risk MDS and whether elements of the adaptive immunity predict response to 5-Azacitidine (5-AZA) treatment.
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PMC11653533
2Distribution of Ficus species throughout the world.
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PMC9429973
Aims: Determine the role of TP53 in JAK2V617F induced genetic and phenotypic modifications.
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PMC5311252
It is tempting to speculate that targeting the entire oncomotif-miRNA group by tiny locked nucleic acids could be an efficient way of re-establishing the G1/S checkpoint in cancers with high expression of oncomotif-miRNAs.
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PMC11765243
The minigene was designed so that in the absence of Cre, the WT form of p110α was expressed from the targeted allele.
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PMC11734514
To assess A549 cells ability in wound healing, a scratch-wound assay was performed on cells treated with 10 µg/mL C. aerizusa extract.8 Cells were seeded in 6-well plates and incubated until 80% confluence.
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PMC9841531
Compound 24 (5 μM) increased the percentage of total apoptotic cells by a factor of 13 from 0.51 to 6.68% when compared to the untreated A-549 cells, indicating that 24 exhibited cytotoxicity against the A-549 cancer cell line via inducing apoptosis.
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PMC10178190
After 24 h, the medium was replaced with medium containing 1 mM dexamethasone (Dex), 5 mg/mL insulin, and 0.5 M 3-isobutyl-1-methylxanthine (IMBx).
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PMC11686380
Single-nucleus RNA sequencing data from the human middle temporal gyrus revealed that GAP43 was co-expressed with SLC17A7, a marker of glutamatergic excitatory neurons, in the intratelencephalic, extratelencephalic and corticothalamic neurons of the cortex (Fig. 1E).
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PMC11082662
In our study, ACER2 knockdown led to a downregulation of SMPDL3B protein, while conversely, overexpression of SMPDL3B alleviated the inhibitory effects of ACER2 (Figure 6A; P<0.05).
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PMC11806106
For the measurement of receptor-arrestin interaction, cells were transfected with 0.5 µg MOR-nLuc, 5 µg sYFP-Arrestin3, and 0.5 µg GRK2 (200 ng DNA/well).
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PMC9429973
Aims: we aimed to study the rare hematological adverse events follwoing the COVID vaccination Methods: Here we are reporting six cases presented to our institute following COVID vaccination within 4 weeks following vaccination, data were retrieved from MRD and events and outcomes were tabulated.
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PMC11786156
In all cases, the mean extravascular Ki-67 index (80.3 ± 17.1%) was significantly higher than the mean intravascular Ki-67 index (68.1 ± 24.6%) (P = 0.012, corrected P = 0.036, Student’s t-test with Holm correction) (Table 2).
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PMC5673955
The main components of telomerase are hTR (human telomerase RNA), comprising RNA template for synthesis of telomeres, and hTERT (human telomerase reverse transcriptase) which possesses reverse transcription activity and synthesizes the telomeric repeats on the hTR template 1.
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PMC10158546
pCDH813A includes a GFP fluorescent reporter protein and a resistance marker to the antibiotic puromycin.
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PMC11682525
These findings underscore the importance of hypoxic preconditioning and warrant further research into the underlying mechanisms, bringing us closer to effective neurological disorder treatments.
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PMC9429973
An overall response rate (ORR) of 62.0% in pts with no prior anti-BCMA treatment (tx) was previously reported in a pooled analysis from phase 1 and phase 2 cohort A at a median follow-up of 7.8 mo.
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PMC11366496
Following the random categorization into distinct cohorts, a cohort of female BALB/c nude mice aged 6–8 weeks underwent anesthesia with three mice in each cohort.
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PMC11790599
After 16 h, the medium containing the transfection mixture was replaced with fresh full medium.
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PMC11618052
An in vitro study evaluated the anti-BRAF efficacy of compounds 8f, 8g, 8h, 8j, and 8l.
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PMC11680562
The experiments successfully synthesized T. dioica dried seeds extract-mediated CuO NPs with an average particle size of 26.44 ± 3.28 nm, displaying a nearly spherical shape as observed through HRTEM.
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PMC10900886
To investigate whether human immune cell subsets are susceptible to EV-D68 infection, human PBMC were inoculated with EV-D68 strains from subclades A, B2, and A2 (previously known as D).
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PMC6461034
○Spin down any remaining liquid sticking to the walls of the peptide solution tube and add that to the peptide/bead mixture.
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PMC11727000
Statistical calculations were performed using the embedded analysis tools in Prism.
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PMC11743975
Image entropy of both the niclosamide and blue light groups was significantly lower than the niclosamide-treated group (Figure 4B).
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PMC11064533
For single nucleotide variation search, the reads obtained for each isolate were mapped against the strain 4972 genome using bwa and called with bcftools (34).
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PMC11721064
The results revealed substantial heterogeneity between cells derived from the two tissue types (Additional file 1: Fig. S3A).
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PMC11760643
Finally, it demonstrated that this mAb can mediate CDC in human leukocytes expressing relatively high cell surface P2X7.
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PMC8345486
Considering the unique physicochemical properties of the metallacarboranes and antimetabolite activity of nucleoside derivatives, we designed and synthesized the adenosine conjugates with metallacarboranes containing iron, cobalt, or chromium as semi-abiotic compounds that influence cisplatin-resistance of cancer cells.
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PMC9429973
Results: The proportion of mutations and ≥ 2 mutations detected by high throughput sequencing was significantly higher than that by SS (P=0.046).
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PMC9429973
In blood cells, relative TMEM244 expression in T cells, both CD4+ (4.7E-04) and CD8+ (6.9E-04) was higher than in any other population of PBMC, including CD19+ B-cells (2.7E-05), CD56+ NK cells (1.2E-04), and CD14+ monocytes (8.8E-05).
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PMC8735881
In human CD4 T cells, GPI-10E8 and its bifunctional derivatives were capable of fully blocking infections of diverse HIV-1 strains regardless of their tropism and the modified cells displayed a robust selective survival advantage over unmodified cells following HIV-1 infection.
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PMC11467800
K562‐IR cells were continuously cultured with 10 μM imatinib mesylate.
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PMC11357960
On the contrary, the percentage change of other 12 genes had no significant correlation with the prevalence rates of NR ( Supplementary Figures 1, 2 ).
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PMC11476666
Since stemness characteristics are based on the expression of specific markers, such as SOX2 and CD44, their protein levels, both in 2D and 3D cell cultures treated with stilbenes, were evaluated by Western blotting.
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PMC5386478
We next examined the effect of TGM2-v1 silencing in MSTO-211H spheroids.
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PMC11701801
Data are presented as the mean ± SD; n = 3 independent experiments.
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PMC11461833
Overexpression of HA-Lamin A in the HEK293 cell line increased the lamin A transcript levels.
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PMC11577421
Liquid chromatography mass spectrometry (LC-MS) experiments were performed in the MSE mode using a Synapt G2 mass spectrometer (Waters) coupled to a nanoAcquity UPLC (Waters).
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PMC11537649
In HCC, miR-152-3p exhibited antineoplastic activity by targeting CDK8.
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PMC11770746
Oxoplatin, ctc-[Pt(NH3)2(OH)2Cl2], (26.7 mg, 0.08 mmol) was stirred overnight at 30 °C with the activated paclitaxel (100 mg, 0.1 mmol) in 3 mL of DMSO.
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PMC11754436
The expression level of miR-362-5p in MG63 cells was increased after transfection with miR-362-5p mimic, *Represent comparison with the mm group. (
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PMC3730428
Circulating levels of TNFα are elevated in patients with MPM, and these levels are reduced following surgical debulking.
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PMC9429973
Epcoritamab is a subcutaneously administered, well-tolerated, bispecific antibody with single-agent antitumor activity in relapsed/refractory (R/R) aggressive B-cell non-Hodgkin lymphoma (NHL).
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PMC10040136
As the immunofluorescence and FACS methods used for MSC counting in this experiment were deficient in terms of accuracy and sample integrity respectively.
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PMC9429973
Patients in ATG cluster 1, showing higher absolute counts of αβ- and activated T cells compared to cluster 2 were associated to a significantly higher 1-year OS (98% vs. 79%, p=0.0023) due to lower NRM (p=0.032) and relapse (p=0.01).
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PMC11718109
Cells were prefixed with 4% (v/v) PFA in Live Cell Imaging Buffer (Life Technologies) for 15 min followed by three times washing with the same buffer.
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PMC9250505
All cell lines have been tested for the absence of mycoplasma.
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PMC8973085
Anal.
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PMC9429973
In detail, it was 1.2 (range, 0-4) and 1.5 (range, 0-7) per patient in PPV- and in PET-MF, respectively (p=0.01).
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PMC11564322
Interestingly, no changes in the PPARγ mRNA expression were observed in the K562 cells co-treated with the c-SRC inhibitor I and the VGVAPG peptide, compared to the control.
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PMC11718817
Interestingly, the effects of IL-18R deficiency and activation with nigericin on both parameters were abrogated at the late time point, after 18 h of coculture.
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PMC11612626
Effect of compound A on immunoproteasome subunit β5i /PSMB8 association with the proteasome activator and immunoproteasome.
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PMC11723947
S1p, q) and co-injected them with the mixture of syngeneic and semi-allogeneic bone marrow.
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PMC11026382
d Cumulative distribution of positions in each epistatic cluster by distance to the DHFR active site in the TYMS R166Q background.
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PMC11129910
The fixed cells were then spun down and resuspended in 2 ml of Carnoy's fixative.
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PMC11049548
Exceptions are the signals of a few molecules, i.e., two UDP-sugars (N-acetyl-glucosamine and N-acetyl-galactosamine, UDP-NAcGln and UDP-NAcGal), which are visible only in the lysates of the immortalized cell lines, and the signals of UDP-glucose (UDP-Glu) and inositol, which are only present in the lysate of HGSOC cells (Figure 1D).
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PMC7812570
GraphPad Prism 6 software was applied to statistically analyze data.
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PMC10317042
For the first time, we identified a transcription factor that regulates EWSR1::ATF1 expression.
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PMC11718817
Murine pancreatic ductal adenocarcinoma (PDAC) cell line Panc02 (RRID:CVCL_D627), which was originally derived from a methylcholanthrene-induced pancreatic ductal adenocarcinoma in C57BL/6 mice , was stably transfected to express ovalbumin (OVA) as described before (PancOVA) .
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PMC11371747
We selected cell lines based on their variation in 5FU sensitivity, while we added cell lines which were adapted to grow under low folate concentrations, hence better representing the physiological folate environment (Lemos et al., 2008; Lemos et al., 2009).
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PMC9429973
starting one week after tumor challenge and continued for 4-8 weekly injections and mice were sacrificed when pre-determined limits of tumor burden were reached.
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PMC11590870
As for MOPH, this is the first research that reports its effect on cancer cells.
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PMC11126803
ERβ, estrogen receptor β; ERE, estrogen response element; ZEB, zinc finger E-box binding homeobox 1.
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PMC11734514
Proteins were extracted from cells using PRO-PREP solution (iNtRON Biotechnology, MA, USA).
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PMC10178190
Moreover, OC cell lines obtained from the same patient before and after development of cisplatin resistance demonstrated higher release of IL-6 after the acquisition of resistance.
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PMC3035438
By plotting the percentage of binding as a function of competitor antigen concentration (Figure 3C), we determined the concentration of competitor required to inhibit 50% of the binding of the free antibody in solution (IC50).
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PMC10093184
Due to the successful implementation of immune checkpoint inhibitors into the clinical practice in the recent years, immunotherapy for osteosarcoma has attracted attention as a potential treatment modality for this lethal disease .
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PMC9429973
Surprisingly, functional studies suggest that consequential effects are enhanced in LNP-SM.
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PMC6487404
Evaluation of the viability of the everted gut sacs The viability and any possible damage of the gut was determined by measuring the release of the cytosolic enzyme lactic dehydrogenase (LDH), as an indicator of cell damage, and analyzing the glucose concentrations both in the mucosal and serosal sides (11, 15).
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PMC11627133
While conventional fluorescence microscopy has provided insights into the organization of cellular compartments and tissue composition, SMLM has advanced our understanding of cellular functions and biomolecular interactions to the molecular level.
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PMC11064533
Sequences of DEGs unannotated by PROKKA (n = 148) along with annotated target genes were analyzed by NCBI blastp, Protein Homology/analogy Recognition Engine (Phyre2) (24), bacterial localization prediction tool (PSORTb 3.0) (25), transmembrane topology and signal peptide predictor (Phobius) (26), and Prediction of transmembrane helices in proteins (TMHMM Server v.2.0.) (
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PMC8973085
Anal.
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PMC11122631
For measuring infrared data, the Jasco FT/IR-4X spectrophotometer was selected.
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PMC10440586
The CD99 expression changes under different treatments.
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PMC7305184
To investigate, if this one statistically significant differences between CTRL and EP group is repeatable and not an experimental error we repeated the experiment.
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PMC4485786
Expression was normalized to that of human CD45.
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PMC11085211
Nevertheless, we did not observe the cleavage of caspase 3, caspase 9, and PARP-1, suggesting a cell death mechanism other than classical apoptosis.
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PMC11019355
An intriguing open question is the relative roles that cis and trans effects play in gene regulatory divergence across species.
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PMC7192625
To consider a larger number of histone modifications and estimate consistency between results on Hi-Cand PCHi-C networks, we calculated Chas of the six histone marks and ATAC-seq from (33) on the FitHiC network for the lymphoblastoid cell line GM06990 (Supplementary Figure S8B).
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PMC3931643
We observed a similar increase in sensitivity when the cells were treated with a structurally distinct asTORi, AZD8055, indicating that these results are specific to mTOR inhibition (data not shown).
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PMC9325715
The SDS-PAGE analysis of obtained complex was performed (Fig. 3).
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PMC9712534
More specifically, we speculate that TOP2B H58Y should be capable of going through its catalytic cycle from the initial DNA binding step to at least the step of religation of cleaved DNA ends, where etoposide acts.
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PMC11723210
Moreover, this unwrapping process is prone to errors, particularly in areas with large phase gradients or low spatial sampling, due to the absence of reliable initial guesses.
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PMC11750696
In parallel, Treg cells deficient in Hrd1 exhibit heightened IRE1α levels, triggering the MAPK p38 cascade, which hinders Foxp3 synthesis and thus undermines Treg stability (57).
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PMC11320834
Symptoms that already appear are nonspecific and include, for example, abdominal pain, early satiety, or changes in bowel habits .
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PMC11457557
Furthermore, study indicates that early glucose management in people with DM is vital in preventing long-term DVC.
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PMC11001582
Both daw and Mp levels were increased in GR36 flies (Fig. 7a), consistent with both the knock-in mouse and patient iPS cell motor neuron data.
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PMC9429973
Pre-emptive Rituximab, given to those with EBV reactivation, reduces the risk of PTLD.
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PMC11593084
LN229, LN229/PDPN, and LN319 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) [Nacalai Tesque, Inc. (Nacalai), Kyoto, Japan].
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PMC7342409
Effect of imatinib on drug sensitivity of GIST882-ir cell line. (
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PMC11285179
CCL28 was up-regulated in all three lung adenocarcinoma cell lines, whereas others were not (Fig. 1D).
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PMC11720808
ns, no significant difference; *P < 0.05; **P < 0.01 compared with the control group.
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PMC7762347
Cytotoxic effects of CYN and MCLR and their mixtures were assessed after 24 and 72 h of exposure on HepG2 cells.
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PMC11706776
Indeed, after KCNN1 silencing, a significant decrease in cell viability was observed in cells treated with 10 nM doxorubicin, whereas no effect was observed in control conditions.
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PMC11591038
These previous findings thus point to the complex, even contradictory, roles of HIF-1 in AD.
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PMC10631132
Statistics are based on ANOVA followed by Tukey’s multiple comparison post hoc test (I n = 3 well replicates, J, K n = 50 analyzed cells, **p < 0.01, ***p < 0.001) Morphology assessment of cells following osteoclast differentiation using CLSM.
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PMC11547972
Clonogenic growth is shown in Figure 4.
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PMC11763111
E The flow plots illustrate the proportion of CD161 marker expression on the surface of T cells in the peripheral blood of four healthy donors.
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PMC10506676
In agreement with our study, Lu et al. stated that in Burkitt's lymphoma, EBNA1 increases the expression of the BIRC5 gene by forming a complex and binding to the promoter of this gene (37).
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