PMCID
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PMC9812014
The alkaloid n-butyl deoxynojirimycin (nB-DNJ) was evaluated in another In vitro study for its synergistic effects when used in combination with interferon-α (IFN) against BVDV.
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PMC11564322
Similarly, in the group co-treated with the c-SRC inhibitor I and the VGVAPG or VVGPGA peptides, a 21.44 and 17.19% decrease in the ERK1/2 protein expression was observed, respectively, compared to the control (Fig. 8F).
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PMC11279598
In this study, we investigated the potential antitumoral effects of MH on estrogen receptor-positive and negative breast cancer.
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PMC9261184
It should be noted that for the main propulsion engines, a new version of this XDF engine is now being offered, and it is suggested to reduce methane slip by approximately half, primarily owing to exhaust gas recirculation.
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PMC9424261
G Endogenous MDM2 was immunoprecipitated by anti-MDM2 antibody and ubiquitination of MDM2 were measured by anti-ubiquitin antibody in Bel-7404 and Bel-7402 cells with or without HERPUD1 knocked out.
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PMC11696659
Luis Fernández: Formal analysis, Data curation, Conceptualization.
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PMC11770130
j p-CerS2 levels in the primary adipocytes upon the combinational treatment of NTS and RhoAi or RhoAa (n = 2–3).
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PMC11621493
The supernatant was adjusted to a concentration of 50 mM sodium phosphate and 300 mM NaCl and diluted to a volume of 50 ml with binding buffer (50 mM sodium phosphate buffer containing 300 mM NaCl) in a 50 ml tube.
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PMC10734950
The intrinsic pathway involves the release of several factors via the mitochondria within the cell .
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PMC10955424
ESI capillary voltage was set to 3000 V, capillary temperature to 350 °C, gas temperature to 350 °C and gas flow to 35 mL/min.
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PMC10170482
In fact, cell geometry is tightly interlinked with the regulation of nuclear shape, cytoskeleton reorganization, chromatin compaction, gene expression, growth, apoptosis, and cell division.
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PMC7369657
As shown in Figure 2(b), exposure to high concentrations of CQDs significantly lowered the MMP levels of 143B cells at 12, 24, and 48 h compared to the control group.
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PMC11742047
Network generated with LINEX2 software.
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PMC11756514
Expression constructs containing full-length open reading frames for Langerin (accession number NM_015717.5) and MGL (accession number NM_182906.4) were cloned into the mammalian expression vector pcDNA3.1/Zeo+ (GenScript), which confers resistance to Zeocin.
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PMC8097906
Western blotting, RT-PCR’s and Immunofluorescence experiments were replicated at least three times with individually passaged cells, and data sets were expressed as means ± standard deviations (SD).
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PMC9429973
Image: Summary/Conclusion: Increased COVID-19 mortality in patients with lymphoproliferative disorders was observed in this study.
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PMC11098378
We confirmed the importance of this residue by mutating C159 to serine and demonstrated that mutant Ceg10 is not capable of breaking viral restriction in LD652 cells.
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PMC10890055
Therefore, only results with 50% extracts are presented.
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PMC9429973
Besides the safety and tolerability primary endpoints, secondary endpoints investigate anti-leukemic activity including ORR according to ELN recommendations, time to response (TTR) and duration of responses (DoR).
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PMC10940855
Real-time PCR reactions were conducted using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, 4304437) on an ABI Quant Studio 6-machine instrument with TaqMan predesigned gene expression assays (Thermo Fisher Scientific, 4331182; ref.
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PMC11347429
Genes influencing T cell signaling and co-stimulation (ICOSLG, HLA-DQB2, and HLA-DQB1) and type I interferon (FADS2, MDGA1, and APOBEC3B) were associated with genetic variation (Data file 13, Figure 9 ).
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PMC10882807
As reported, hsa_circ_0005397 is a kind of circRNA originating from RHOT1 that promotes HCC by regulating the miR-326/PDK2 axis .
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PMC11617590
These data suggest that SFN has a potential anticancer effect.
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PMC9111184
Spectral regions were considered for analysis, (a) 1300–950 ​cm, (b) 1700–1600 ​cmFig.
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PMC7022782
The high peak at 785 nm was due to unloaded NPs scattering whereas the peaks in the 800–850 nm wavelength range were due to ICG emission.
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PMC11599565
Western blots were subjected to densitometric analysis.
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PMC11319300
We discovered that not only was the coding sequence of MYEOV strongly conserved across all seven primate species considered, but there was also conservation of the sequence (∼1 kb) where the human MYEOV-3′-putative enhancer is located (Supplementary Figure S5).
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PMC11359735
Day 15 to 18 cultures, containing largely mature gametocytes, were used for mosquito feeds: Cultures were centrifuged at 108g for 4 min and the parasite pellet was resuspended to final gametocytemia of 0.3% in a mixture of human O RBCs supplemented with 50% v/v human serum.
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PMC8358176
Apoptosis is an important issue in toxicological studies, and tumor necrosis factor (TNF) family members execute crucial roles in apoptosis and inflammation.
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PMC9429973
Consistent with AEs often observed during AZA + VEN therapy (DiNardo CD, N Engl J Med, 2020), the 5 most common AEs regardless of relationship to study treatment were constipation (all grades [gr]: 39%; gr ≥3: 0%) and hematologic events, including anemia (33%; 28%), decreased platelets (33%; 33%), neutropenia (39%; 39%), and febrile neutropenia (gr: 50%).
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PMC9429973
CP-673451 was tested on the selected cell lines.
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PMC11726183
The descriptors evaluate general parameters like Lipinski rule of five and other parameters like log P, BBB (blood–brain barrier) permeability, human oral absorption, HERG binding, and log S as shown in Table 4.
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PMC3675084
BPR0L075 treatment (10–100 nM for 24 hours) induced changes in cyclin B1, BubR1, MPM-2, and survivin expressions in OVCAR-3/OVCAR-3-TR cells (A) and SKOV-3/SKOV-3-TR cells (B).
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PMC11592008
However, in some experimental settings, the use of PKs can be challenging because of the limitations related to difficulties in genome editing approaches, short lifetimes, and the incapability of the long-term subculturing of primary cells.
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PMC9777812
All of the cells were maintained at 37 °C in an atmosphere containing 5% CO2.
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PMC9429973
The most upregulated genes in tMZL compared to de novo DLBCL were TNFRSF13 and CXCL13 (both logFC ≥2.0, p<0.05) while the most downregulated gene was FN1 (logFC -1.6, p=0.0057).
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PMC8526701
I WT and PUMA-KO GIST-882 or GIST-T1 cell lines were treated 0.5 μM GSK602 for 24 h. Cell viability was analyzed by colony formation assay.
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PMC7215832
Antibodies were diluted in a mixture of Rockland buffer (Rockland Inc, Philadelphia, PA, USA) and phosphate-buffered saline (PBS) supplemented with 0.05% Tween 20.
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PMC9429973
The potential structural effects of amino acid changes were predicted by PremPLI (protein-IMiD complex) and INPS-MD (only protein) tools by prediction of protein stability free energy change (ΔΔG).
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PMC11489175
Following USP1 inhibition, a subset of BRCA1/2 wild-type as well as BRCA1/2 mutant cells show a S-phase specific DNA damage, decreased DNA synthesis, reduced replication fork speed, and activation of the ATR-Chk1 signaling cascade.
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PMC11764090
Chorispora bungeana (Chorispora bungeana Fisch.
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PMC9792775
Patients with sarcoma may achieve acceptable curative effect that undergo chemotherapy alone, while for most sarcomas, the outcome is often unsatisfactory and also accompanying adverse effect, and therefore the comprehensive treatment of new chemotherapeutic regimens was needed and crucial to improving the survival rate of sarcoma patients.
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PMC11718274
Screening viability of normal HSF cells and cancerous A-431 cells using SRB demonstrated a clear differential response to varying concentrations of Y2O3NPs.
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PMC9429973
On 13 of November she presented with extensive petechiae and purpura and was again started on steroids and was given the courses of intravenous immunoglobulins (IVIG) without any sustained response.
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PMC11767725
For instance, hyperglycemia in T2DM leads to the formation of advanced glycation end products (AGEs), which bind to the receptor for AGEs (RAGE) on keratinocytes and immune cells, promoting oxidative stress and inflammation while facilitating Staphylococcus aureus colonization in atopic dermatitis.
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PMC9429973
Additional patients, updated data and longer follow up will be presented.
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PMC6337547
LAMP1 acts as a barrier to soluble hydrolase, preventing cell damage and death caused by the release of luminal contents into the cytoplasm.
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PMC8584319
Many reports have shown that treatment with these drugs completely reduces residual 4E-BP1 Thr37/46 phosphorylation and AKT Ser473 phosphorylation .
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PMC11463018
White solid, 60.2% yield.
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PMC11467800
Phenotypic plasticity in cancer cells also contributes to therapeutic resistance.
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PMC11545737
Inhibition of ROS production by N-acetyl-l-cysteine blocks NLRP3 inflammasome and pyroptosis .
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PMC11756514
The Langerin- and MGL-encoding vectors and an empty vector (mock, i.e., the pcDNA3.1/Zeo+ vector alone) were introduced into the adherent HEK293 cell line (the cell line is adherent under the conditions used with FBS) by transfection.
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PMC11502962
ImageJ software was used to scan the stained tissue chip and count the stained cells.
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PMC11534377
Following 2 Gy IR treatment, there were noticeable alterations in the arrangement of actin filaments, and the mitochondrial staining appeared slightly less homogenous.
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PMC11588085
Ovarian CSC markers have been identified, including the surface markers CD44 and CD24 (7–10).
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PMC11544122
For tube #1, 20 µL of 1.25–2.5 mM peptide was added, and 10 µL was then transferred to tube #2.
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PMC9429973
Solubilised E from a CR1-H/H donor converted iC3b more effectively to C3dg compared to E from CR1-H/L donors.
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PMC11025866
Interestingly, though one compound is an HDACi and the other is not, both VPA and VPM find similar applications as drugs used to treat neurological conditions, such as epilepsy and mood disorders.
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PMC11527437
The targeted antigen is a key consideration for TCR-T; optimal tumor antigens are tumor specific, to avoid toxicity in healthy tissue, and essential for maintenance of the malignant phenotype.
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PMC11122631
To date, more than 60 marine prostanoids have been identified from the genus Clavularia.
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PMC6742971
The frequency of the resulting colored spots corresponding to the cytokine producing cells was determined using the Elispot Bioreader 5000.
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PMC11462673
S4A-C).
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PMC11119602
In our study, however, it appears that TG2 may be able to interact with LC3 through the C-terminal 27-residue region located on the β-barrel domain B1, which is present on TG2-L and TG2-S, as well as multiple short linear motifs harboured on the exposed flexible loops of LC3 (Figure 7).
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PMC11706491
This phenomenon is readily observable using an active growth marker like CRIB .
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PMC11697065
For differential gene or protein expression analysis, DESeq2 package was applied to RNA-seq data for both cell pools and clones as described above.
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PMC11473750
It will be important to determine whether MYCN and RNF121 indeed bind together at this membrane and that this site of binding is necessary for its oncogenic effects.
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PMC9429973
NGS analyses showed that the most recurrently mutated genes in del-3’IGH CLL were NOTCH1 (30%), ATM (20%) and TRAF3 (13%).
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PMC10988555
Melting curves were analyzed in a Corbet RotorGene 6000 (Biorad, Spain) by a first incubation step at 37 °C for 5 min followed by a ramp from 37 to 95 °C with 90 s hold in the first step and 5 s hold in the next steps with green acquisition.
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PMC9250505
Arrow indicates fold reduction in respective PARP inhibitor IC50 in the presence of NPB (n = 3).
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PMC5839394
The protein folding and generation of reactive oxygen species (ROS) as a byproduct of protein oxidation in the ER are closely linked events, and persistent oxidative stress and protein misfolding initiate cell death.
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PMC8662250
ELISA for human TNF‐α (sensitivity: 1.7 pg/ml assay range: 15.6–1,000 pg/ml), IL‐6 (sensitivity: 2 pg/ml assay range: 16–1,690 pg/ml), IL‐1β (sensitivity: 2 pg/ml assay range: 33–1,400 pg/ml), and IL‐18 (sensitivity: 6.25 pg/ml assay range: 25.6–1,000 pg/ml) (Thermo Fisher Scientific Inc. [Monza, Italy]) were carried out with undifferentiated Caco‐2 cell supernatant after treatments at 24 h according to the manufacturer's protocol.
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PMC6461034
ABL1 is the most activated kinase, with relatively equal contributions from both analysis arms.
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PMC6617630
Raji cell line, which harbors a single MYC rearrangement, was the most sensitive to I-BET, JQ1, and OTX with nearly 95% inhibition of thymidine incorporation (Fig. 2a–c).
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PMC11742231
In this case, it would be useful to evaluate the performance of the model in comparison to other attention mechanisms or without any attention mechanism to further validate the effectiveness of multi-channel attention.
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PMC11033180
Data are mean ± SEM, n = 8 cultures from 2 independent experiments.
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PMC6182045
Briefly, rgp120 in cell culture supernatant was captured on 96 well Maxisorp plates via a mouse monoclonal antibody (34.1) to an N-terminal purification tag (23), incubated with a 2 μg/mL dilution of purified goat-anti-rgp120 polyclonal antibody, and detected with a 1:3,000 dilution of bovine-anti-goat HRP conjugated polyclonal and OPD substrate.
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PMC4695073
The staining intensity of cancer cells (excluding noncancerous cells, especially lymphocytes) were graded on a scale of 0 to 3 (I0, I1-3): negative staining (0), weak staining (1), moderate staining (2), and strong staining (3).
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PMC11661024
The cell survival rate of the HUVEC and HeLa cells treated with two postbiotics shows that the indigenous L. fermentum KH and L. fermentum CH had low toxicity effects.
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PMC11297139
We found distinctive foci of GFP-ARID1A inside the nucleus of A673 knock-in cell line (Supplementary Fig. 6d).
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PMC11095939
Based on our comparative analysis of RNA-seq data from control and Mecp2 cKO livers before PHx, only 90 upregulated and 128 downregulated genes (log2 |FC|>1.5, p<0.05) were identified, which did not enrich any GO terms with adjusted p<1 × 10, further supporting the notion that liver-specific deletion of Mecp2 does not cause liver abnormalities in 3-month-old mice.
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PMC9429973
AIHA was idiophatic in 58 cases and secondary to lymphoproliferative desorders in 4 cases, autoimmune disorders in 25 cases,3 cases were secondary to Methyldopa.
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PMC9429973
Results: The total number of evaluable pts was 298.
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PMC11521786
3LL-R3 cells were treated with immune sera (heat-inactivated and 1:20 diluted).
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PMC11087766
Quantification of >100 infected cells per condition shows that a maturing phage nucleus greater than 0.5 micron in diameter formeds in the majority (84%) of infected cells expressing the nontargeting guide (Fig. 4F).
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PMC11549612
To analyze the diagnostic and prognostic potential of the BOLA gene family in kidney renal clear cell carcinoma (KIRC).
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PMC11371747
CCRF-CEM (a human acute lymphocytic leukemia (ALL) cell line) is used as a control and set at 1.
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PMC8662250
In perspective, this could imply that a CBD‐mediated defence against COVID‐19 at the gastrointestinal tract would result in a possible control of the SARS‐CoV‐2 enteral route of infection.
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PMC7053076
Statistical significance was assessed using test t and one-way ANOVA and a post-hoc multiple comparison Student–Newman–Keuls test. **
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PMC7582629
Three different ratios of cancer cell/leucocytes 50/50, 1/10, and 1/100 were additionally studied.
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PMC11670954
BA alleviated the LPS-induced decrease in TEER in Caco2 cells after treatment for 24 h. (C) BA alleviated the LPS-induced increase in FD-4 flux in Caco2 cells on day 22.
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PMC9436459
This type MDAAs is distributed in 11 species including A. heterophyllus, B. oblongifolia, D. barteri, M. alba (M. alba var.
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PMC10723784
Cytotoxicity induced by a combination of cannabinoids plus carboplatin was comparable to the cytotoxicity induced by carboplatin alone in non‐carcinoma cells, ARPE19 cells.
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PMC11763111
G The histogram depicts the difference in CD62L MFI levels between 4KO CAR-T and 4KO-LLT1 CAR-T cells after 24 h of CCRF-CEM stimulation, as assessed by flow cytometry (n = 3).
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PMC10759659
Hep3B and SK-HEP-1 cells stably expressing Cas9 were established by lentiviral transduction with Cas9 plasmids (Lenti-Cas9-2A-Blast, 73310; Addgene).
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PMC11551844
The blank was the microplate wells (in triplicate), which consisted of the growth medium and DMSO (0.7%).
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PMC10571053
Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen, Germantown, MD).
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PMC11792766
No. 14190144).
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PMC11666785
As per the Nyquist-Shannon theorem, the distance between two neighboring point emitters (here, single molecules) should be two times that of the desired resolution.
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PMC11228511
Confocal fluorescence microscopy image of aortic SMCs from a 10-wk-old Lmna mouse stained with an antibody against lamin A/C (yellow).
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PMC11741906
To develop gene ontology for differentially expressed miRNAs in cisplatin-resistant ovarian cancer cells, over-representation analysis in the miEAA software was used .
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