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OTAR3088
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BioData-CellLine_annotated

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PMC8567602
Here, we found clear coprecipitation, although we also observed reduced levels of both SMARCB1 and SMARCC1 proteins in the nuclear extracts (input sample) following expression of the fusion compared with controls that expressed tagged SS18 (Fig. 5C).
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PMC11731161
As shown in Fig. 5E, elevated levels of USP7 were associated with shorter overall survival (OS) in patients with MM across CoMMpass, GSE24080 as well as GSE2658 cohorts.
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PMC8567602
Mouse strains were maintained on a mixed C57BL/6 and SvJ background with littermate controls and roughly equivalent distribution of sexes.
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PMC11698348
The data shown are from a single representative experiment out of three experimental repeats. (
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PMC11745823
The CCK‐8 is a commonly employed reagent in tests that evaluate cell viability and proliferation, as it measures the metabolic activity of cells, thereby indicating their viability.
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PMC11802929
Transcriptome, TCR, surface protein levels, and antigen specificity were simultaneously analyzed for each cell.
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PMC11544771
We found that the combined treatment more effectively inhibited cell growth relative to the treatment with CPX or PRE alone.
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PMC9429973
Determining fluorochrome composition allowed quantifying the proportion that each of the 5 samples contributed to the entire tumor load.
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PMC9429973
The primary efficacy endpoint was the overall hematologic response (partial and complete), as determined by platelet, hemoglobin and neutrophil counts by 16 weeks after the initiation of EPAG.
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PMC9429973
No association was detected between age or gender and HF mortality.
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PMC8662250
Furthermore, as demonstrated in macrophages and in lung epithelia, SARS‐CoV‐2 SP can induce a proinflammatory response in infected cells by triggering inflammasome NLRP3 complex activation (Sebastian et al., 2021).
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PMC11706491
To analyze Atf1 phosphorylation, cells were grown in EMM to log phase (OD600 = 0.5).
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PMC11604830
The use of nanoparticles has positively impacted the research in this field over the past decades because of the many advantages that nanosystems may confer to imaging probes in terms of enhancing the sensitivity in contrast detection, improving pharmacokinetics, and facilitating multimodal imaging capability, thus significantly boosting the overall potential of such agents to design more and more advanced and sophisticated diagnostic/theranostic procedures. ,
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PMC10547921
Although there are currently few PDC drugs on the market, there have been cases of withdrawal due to serious safety issues.
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PMC10999934
In order to investigate the functional significance of the potential biomarker, we gathered genes that interact with it from three protein‒protein interaction (PPI) network databases: STRING , BioGRID and HPRD .
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PMC11769522
Keratinocytes express a large variety of cytokines (interleukins, IL) and chemokines (chemokine ligands with a C-C and a C-X-C motif), which secrete pro-inflammatory signals to recruit immune cells to the site of injury, infection, or inflammation .
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PMC9622879
However, these usually require substantial computational time, especially for a large community including many homes.
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PMC9118379
Second, the original search score from a search engine represents the similarity between a variant spectrum and a theoretical spectrum generated from a specific peptide in the database and thus is unlikely to be changed for a given spectrum–peptide pair regardless of which database the peptide is from.
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PMC11575040
Following RNase treatment, 50 mL of propidium iodide (Boehringer Mannheim, Mannheim, Germany) was added, and the cells were incubated at room temperature for 10 min with light protection.
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PMC11594588
However, a proportion of MIs, ranging from 1% to 14%, occur in the absence of coronary artery obstruction .
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PMC7305184
While with gene therapy new genes and products are expressed within the host cell, DNA vaccination induces immune response of host organism.
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PMC11365427
Based on these findings, we propose the hypothesis that phenylalanine is an important nutrient that supports MM growth.
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PMC7642379
These variants were then functionally annotated using snpEff (4.1) and snpEff reference GRCh37.75.
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PMC11740508
S1200, SelleckChem®) given by intraperitoneal (IP) injection.
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PMC9577200
Cultures in Luria-Bertani (LB) broth medium containing 50% glycerol as a frozen stock (−80°C, RS Biotech freezer, Richmond Scientific Ltd., Lancashire, PR6 0RE, Great Britain).
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PMC11552389
By targeting FOXN3, we may potentially disrupt key pathways driving resistance, leading to improved treatment outcomes for individuals suffering from drug-resistant melanoma.
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PMC7570809
Two duplicates were set for the assay.
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PMC11742670
Each feature is accompanied by a weight, representing its influence on the prediction where the green bars illustrate the features that make the prediction stronger, whereas the red bars show features that provide evidence against it.
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PMC9577200
There are a few reports about the ratios of the GO:CS combination.
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PMC10419319
In this study, the potential of isoalantolactone as a glycolysis inhibitor was investigated to overcome cisplatin resistance in OC.
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PMC10743717
Here, 17β-HSD10 acts as a platform to play a scaffolding role only as shown in the cryo-EM density map of the mtRNase P complex (see Figure 1 of Ref. ).
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PMC11055510
A2780 is a human ovarian cancer cell line that is commonly used in experimental studies in the fields of cancer biology and drug discovery.
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PMC11367146
It was observed that the level of phosphorylation of MAPKs (ERK1, p38, and JNK) and STAT3 was elevated by 60.07 %, 52 %, 49.05 % and 49.90 % compared to the untreated group after induction of SW982 cells with IL-1β, thereby demonstrating the activation of kinases.
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PMC5443223
This, therefore, indicates that CEP164 may be a potential target to improve the outcome of radiotherapy in heart rhabdomyosarcoma treatment.
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PMC11672617
The cells were subcultured at two-day intervals at a seeding density of 1 × 10 cells per 24-well plate in RPMI medium with 10% FBS.
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PMC2193049
The UPR technique exploits this natural linear arrangement of the Ub gene and relies on a linear fusion with Ub located between a reference protein (here GFP) and a protein of interest (Fig. 1).
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PMC9429973
Out of 954, 592 detected beta thalassemia major, 304 Thalassemia trait, 24 Thalassemia intermedia 24, 9 sickle cell,12 sickle beta thalassemia,7 E Beta thalassemia and 6 D beta thalassemia patients.
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PMC9429973
Methods: We queried the Nationwide Readmission Database 2018 using ICD-10-CM codes to identify all adult patients admitted with a diagnosis of CD.
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PMC11740207
Telomere shortening is a fundamental mechanism underlying the HE.
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PMC11683130
In humans, most (24 out of 36) serpins are inhibitory ( Table 1 ).
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PMC7185206
Our results additionally suggest potential novel therapeutic approaches to PD-(L)1 inhibitor-resistant tumors.
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PMC11021472
This revealed that the strong inhibition of cell growth upon miR-150 overexpression was not rescued by overexpression of circZDHHC11 (Fig. 3C,F).
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PMC11040965
miR29b was detected in situ using the miRNAscope HD Assay Red (Advanced Cell Diagnostics, Newark (CA), USA) and an miR29b-specific probe (SR-hsa-miR-29b-3p-S1 MIMAT0000100, Advanced Cell Diagnostics).
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PMC11391695
The mice were allocated arbitrarily into four groups and subsequently administered tail vein injections containing different types of substances: normal saline, PEI/NPs@M, nc/PEI/NPs@M, and pcDNA/PEI/NPs@M (Fig. 7A).
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PMC11720107
Fluorescence was measured with excitation at 530 nm and emission at 590 nm.
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PMC11792888
Preliminary results analyzing mRNA expression in the human breast cancer cell line MDA-MB-231 suggest that apoptosis may be the preferred mechanism of cell death for this natural extract in general.
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PMC11607638
Although the detailed reasons for SKR activation‐induced persistent calcium spikes are not known at the present, more insights could be gained from receptor single‐molecule force spectroscopy and super‐resolution microscopy , , studies in the future.
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PMC11354225
It is worth mentioning that PDT is not authorized by the FDA for the treatment of melanoma skin cancer due to the resistance of this type of cancer, caused by melanin competing with the photosensitizer for photon absorption , unlike NMSC .
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PMC11264242
To test this possibility, we first treated SYO-1 cells with the small molecule PP2, a SRC family kinase (SFK) inhibitor which preferentially blocks the action of FYN (Hanke et al., 1996).
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PMC2010673
They reacted strongly against 791T cells and another osteogenic sarcoma, 788T, and more weakly against a further 2 from a total panel of 10 osteogenic-sarcoma lines.
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PMC11344246
MCAS was grown in alphaMEM supplemented with 10% FBS (Cytiva) and 1% Glutamax (Gibco); JHOM-1 in DMEM:F12 (Gibco) supplemented with 10% FBS, 1% Glutamax (Gibco) and 1% NEAA (Gibco); RMUG-S in Ham's F12 (Gibco) supplemented with 10% FBS; BJ in EMEM (Gibco) with 10% FBS and HFF-1 in DMEM (Gibco) with 15% FBS.
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PMC11533140
Data are means from three independent experiments, in which the variation is less than 20%.
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PMC5916734
Denotation: IRES – the amplicon consisting of the IRES fragment and the adjacent DHFR ORF fragment; FIX – the amplicon from the ORF of the FIX gene.
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PMC11348300
A white LED was used in synch with the laser to avoid overlap with the acquisition window, and the color background acquisition was performed continuously.
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PMC11615218
Several studies have reported that lower frequencies of PD-1, LAG3, and TIM3 expressed by infused and engrafted CAR-T cells are correlated with better disease control [23–26].
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PMC11765988
Some authors have concluded that topical steroids may serve as an independent risk factor for HL that depends on the dosage, duration, and potency of the treatment .
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PMC10114490
Cells were maintained at 37 °C with 5% CO2 and were regularly examined with an Olympus CKX41 microscope.
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PMC11763111
CAR-T cells were irradiated to prevent cytokine release and cytolytic activity against CD38 cells and then subjected to a mixed lymphocyte reaction (MLR) with primed PBMCs (Fig. 6A).
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PMC11049548
Furthermore, PC changes in the progression from non-tumoral ovarian surface epithelial cells versus carcinoma cells and dysregulation of choline metabolism have been proposed as hallmarks of novel cancer .
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PMC11727062
Despite their diverse chemical properties and distinct modes of action, herbal monomers share comparable mechanisms that contribute to their antitumor efficacy.
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PMC10604092
Additionally, we observed an increase in the blood levels of PAI-1 in the SLC-transplanted rats; this increase was approximately 5-fold higher than that in the control rats.
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PMC9545474
Deposition Number 2169506 contains the supplementary crystallographic data for this paper.
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PMC11789597
A heatmap showing the normalized median expression of five indicated markers in seven identified cell subsets.
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PMC11604768
In addition, we demonstrated that individual cell lines produce distinct basal and stimulus-dependent TRE-MPRA signatures, highlighting the utility of this biologically-responsive reporter library for use in cell-type specific applications.
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PMC11597167
The protein was prepared using the Protein Preparation Wizard tool.
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PMC11087301
Once the modulatory balance between self-renewal and differentiation is eradicated, uncontrolled CSCs finally cause tumorigenesis.
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PMC7823217
Typically, at the time of diagnosis this type is completely diffused beyond the ovary.
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PMC11361748
These data suggest that K17 may act as an amplifier of PKCα activity in stressed skin keratinocytes in vivo. (
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PMC11680391
Good consulting from the herd veterinarian consists of several elements, e.g., herd-specific goals, tailored communication, trust, and understanding; therefore, we analyzed the level of agreement.
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PMC10170482
Studies estimate the diameter of collagen fiber to be about 200–1000 nm.
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PMC9429973
Thirty five subjects (65%) suffered from febrile neutropenia.
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PMC7802142
In the future, it could be beneficial for large-scale production of ReN-NV for further studies and non-scaring alopecia therapies.
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PMC9429973
Summary/Conclusion: Location of bleeding events in patients on antiplatelets or anticoagulants is influenced by age and alcohol use but not smoking.
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PMC10818573
The results were observed after 5 min.
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PMC11344246
We identified five candidate genes and performed siRNA knock downs in MOC cell lines and fibroblasts.
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PMC11585254
GPCR signaling is tightly regulated by GPCR kinases and β-arrestins (Komolov and Benovic, 2018).
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PMC11476004
There has been little interest in the identification of other chromosomal instabilities in dogs, identified using the SCE and SCGE assays.
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PMC11727638
Flow cytometry revealed that CD19 expression on TK and KML-1 cells cultured for 2, 7, and 10 days in the Cell Domes was similar to that of those cultured in 2D flasks (Figure 6a and Table S1, n = 3, p > 0.05).
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PMC11536589
Each cluster exhibited distinct signature genes, and we noticed that elevated expression of several genes associated with ferroptosis inhibition in the Cluster 5, including CP, MGST1, GCLC, GSTM1, SLC40A1, and ACSL3 (Fig. 1B).
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PMC10747139
In this context, this review focuses on the Ru complexes in clinical trials and on the most promising drugs in preclinical studies as antitumoral and antiviral agents, thereby highlighting their importance in the treatment of different types of cancer and their potential as antiviral drugs.
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PMC11597167
The possible cytotoxic activity of quercetin, rosmarinic acid, EGCG, oleuropein, and gallic acid determined to be most active in terms of the anti-Alzheimer effect was evaluated in SH-SY5Y human neuroblastoma cells by the Alamar Blue method.
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PMC11721064
For this study, single-cell RNA sequencing (scRNA-seq) data were procured from the GEO database, specifically datasets PRJNA891516 and GSE122043.
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PMC10522430
A subsequent 3-hour incubation at 37°C ensued, resulting in the formation of formazan.
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PMC11011837
After incubation of the cells under physiological conditions for 48 h, 1 million cells were collected, pelleted, and washed twice with PBS.
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PMC4277423
Luciferase activity in 50 µl of cell suspensions was measured by a BrightGlo Luciferase assay according to the manufacturer's instruction (Promega).
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PMC4443654
We constructed cell-type-specific models of nuclear organization, as reflected in Hi-C-derived eigenvector profiles, to discover the most influential features underlying higher-order structures.
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PMC11585565
Conservation analysis demonstrated that residues Gly492 and Thr495 are highly conserved within the CNNM protein family and CNNM4 across diverse organisms. (
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PMC9370419
Estradiol inhibits the Hepatitis C virus (HCV) transmissibility and HCV RNA replication through estradiol interaction with ER-α .
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PMC11750712
Other targets such as CD30, CD37 or CD70, which have none or limited expression on normal T cells can be used; however, these are only expressed on a small proportion of PTCL cases.
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PMC10587429
Mammalian expression construct for Luciferase (pMMP-LucNeo, Stephen Lessnick, MD, Nationwide Children’s Hospital, Columbus, OH). -
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PMC10831439
CD19-CAR-iNKT cells exerted robust tumor control and elicited cross-priming of host CD8+T cells, enhancing long-term tumor control.
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PMC11612315
Exendin 9–39, a synthetic peptide, functions as a specific and competitive antagonist of GLP-1.
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PMC11066331
VIP score then confirms that cumulative contribution of variables is necessary for the correct classification.
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PMC9878562
5-7 days later, when >80% confluence was achieved, cells were detached using Accutase (Biological Industries).
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PMC10317042
The calculated IC50 values are shown in Table 1.
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PMC6034753
First identified during an outbreak of MD, CAV infection leads to the development of anemia, immunosuppression and increased mortality .
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PMC11666785
In addition, we have counted the number of molecules that represent the actual structure (such as, actin filaments) and background (molecules in between the filaments).
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PMC11589157
The Hi-C libraries were amplified through 10–12 cycles of PCR and sequenced on an Illumina Novaseq6000 platform with 2 × 150 bp reads.
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PMC11525028
Orthotopic xenograft osteosarcoma models were established by injecting 1×10 indicated cells suspended in 20 μL PBS into the proximal tibia through the anterior tubercle cortex.
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PMC8345486
The purity of compounds used for cell culture treatment was ≥98–100%.
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