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PMC10772747
In addition to inducing cell death, the increased anticancer effects can also occur through cell cycle modulation.
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PMC11186948
Three chromatinopathies are known to be linked to HDAC genes so far (Simon et al. 2010; Deardorff et al. 2012b; Wakeling et al. 2021), and only one of them is caused by mutations in a class I HDAC gene: Cornelia de Lange syndrome 5 (CdLS5), whose affected patients are carrier of pathogenetic variants in HDAC8 (Deardorff et al. 2012a; Kaiser et al. 2014; Parenti et al. 2016).
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PMC11621493
PE and PC subspecies are labeled according to their acyl-chain composition.
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PMC11055443
By increasing the proliferation and differentiation of epithelial cells, it helps to protect the integrity of the intestinal epithelial barrier (37).
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PMC11015054
After cell adhesion, the cells were transferred to transwell chambers.
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PMC11653533
Again, the presence of sterols and triterpenoids including betulinic acid, lupeol, stigmasterol and β-sitosterol-3-O-β-D-glucoside in fruit extract has shown significant anti-inflammatory activity (El-Fishawy et al., 2011).
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PMC10565698
HCCLM3 cells were transfected with lentiviruses.
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PMC11063646
CCD with Factors and experimental responses (Evaluation parameters of ANG 1-ANG 11). (
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PMC6442998
YAP is an important transcriptional co-activator and its activity is tightly regulated by multiple upstream pathways.
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PMC11637284
Even though OM-IM contact sites are widespread and functionally important, not much is known about the underlying molecular interactions required for their formation and maintenance.
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PMC9429973
Prevention of pneumocystis pneumonia (PCP) (89.1%), antifungal (37.0%) and antibacterial (87.0%) therapy were carried out in the treatment of COVID-19.
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PMC11291490
The virus has demonstrated the ability to replicate within human cell types, including nerve cells, liver cells, and kidney cells.
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PMC11089031
So, we used those two cells to study the role of ZNF692 in the migration of ccRCC cells.
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PMC11766332
Alongside, LFNG plays a critical role in biological processes involving glycosylation because it encodes beta-1,3-N-acetylglucosaminyltransferase lunatic fringe, a member of the glycosyltransferase superfamily .
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PMC11128598
In fact, previous studies have shown a synergistic effect with HDAC inhibitors and many of these treatments (56–58) with several combinations reaching clinical trials (30).
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PMC9429973
J. Rytlewski, J. Fuller, D. R. Mertz, C. Freeman, S. Manier, N. Shah, T. B. Campbell Bristol Myers Squibb, Princeton, NJ; Moffitt Cancer Center, Tampa, FL, United States of America; CHU de Lille, University of Lille, Lille, France; University of California San Francisco, San Francisco, CA, United States of America Background: The anti-B-cell maturation antigen (BCMA) autologous chimeric antigen receptor (CAR) T cell therapy idecabtagene vicleucel (ide-cel; bb2121) has achieved deep and durable responses in relapsed/refractory multiple myeloma (RRMM) patients (Munshi, N Engl J Med 2021).
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PMC11271800
It also decreases the infiltration of Treg cells and expression of PD-L1, thus providing an immunological benefit.
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PMC10936243
So, I think it [bedding] provides some comfort, but I don’t think it’s related much to pain, as in the surgery” [D091]. “
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PMC11467964
For instance, Chen et al. found that through the TGF‐β signaling pathway, GDF15 can promote osteosarcoma migration and invasion.
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PMC11655536
To detect whether the number of lysosomes was increased, we stained HA-treated MM cells with lysotracker dyes that labelled lysosomes with red fluorescence.
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PMC11092382
These studies consistently demonstrated that sophocarpine exerts antiarrhythmic effects through the inhibition of the hERG channel.
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PMC11352311
The intersecting targets of esculin and RCC were analyzed using the jvenn tool in bioinformatics .
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PMC11700247
E-F) Gene expression of granzyme B (gzmb) and perforin 1 (prf1) in irradiated and nonirradiated tumor 5 days after RT. (
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PMC9927933
The DEP domain–containing mechanistic target of rapamycin (mTOR) interacting protein (DEPTOR), also known as DEPDC6 (DEP domain–containing protein 6), serves as a negative regulator of both mTOR complex 1 (mTORC1) and complex 2 (mTORC2) (23).
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PMC10955424
In Fig. 2B, IL-22 is shown as the most prominent cytokine, found in higher concentrations in the cirrhosis ascites.
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PMC11798926
Loss of SynDIG4, and the subsequent decrease in extra-synaptic GluA1, has been found to significantly impact synaptic plasticity in the hippocampus.
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PMC11357960
Base on the percentage change in mRNA levels, the relationship between the expression of SUMO machinery components and outcomes of anti-PD-1 immunotherapy, and the influencing factors of SUMOylation were evaluated.
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PMC11740907
Figure 8A and Table 5 displayed the survival rates of mice that were fed various dosages of cisplatin (30 µg/kg), pure P5W30 (30.87 µg/kg), and P5W30-BW-SLNs (14.72 µg/kg).
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PMC6114978
None of the 13 cell lines evaluated by Sanger sequencing demonstrated mutations in the above mentioned serine residues (Supplementary Figure 4B). (
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PMC7338939
Therefore, targeting resistant melanoma cells within tumors is necessary (26).
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PMC11612315
One-way ANOVA followed by Dunnett’s multiple comparison test (ns, not significant; ****p < 0.0001, compared with the control, pH 5.5).
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PMC11473843
Scale bars: 100 μm. (
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PMC6889484
Proviral DNA from cell cultures in which virus replication peaked in round 1 and round 6 were analyzed for G-to-A hypermutation by sequencing an 896-nt Vif region.
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PMC11742431
For example, Zhang et al., reported a covalent nanobody‐based degradation strategy, GlueTAC (a covalent nanobody‐based PROTAC strategy), for targeted membrane protein degradation with high specificity and efficiency.
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PMC11742231
By assigning attention weights to different parts of the drug graphs, the model highlights which molecular features are most influential, providing insights into the mechanisms of action at a molecular level.
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PMC11781181
This study included the blood samples of patients with SJS/TEN, erythema multiforme (EM), and healthy individuals.
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PMC10671321
About half of the WT distal peptidic sequence is conserved in the mutated sequence.
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PMC9985266
Cells were then incubated in the dark for 30 min, and the staining solution was discarded.
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PMC10705095
Cells were transfected with HBQ1 plasmid DNA or shHBQ1 for 24 h, trypsinized, and resuspended in a medium.
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PMC9429973
On the contrary, radiotherapy is less accessible in certain hospitals and presents a greater incidence of secondary cutaneous adverse effects.
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PMC11258145
This knowledge has allowed the use of curcumin as a photosensitizer in photodynamic therapy.
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PMC11794588
Consequently, fractions 34 and 37 underwent further separation with the use of a mobile phase comprising acetonitrile and water.
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PMC11481779
It is crucial in the decoder to use various options for the dilation parameter - it has been done in the previously mentioned papers.
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PMC11756907
Three samples were taken from each stage of the research.
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PMC9429973
Similar results were obtained with three treated group relative to control group (all p<0.05).
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PMC11011837
The raw data for metabolic and lipid analysis were processed using MS-DIAL software (version 4.9).
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PMC10873328
The difference in TGFBI expression was examined between cisplatin-sensitive and -resistant cells, and the effects of TGFBI interference on cell apoptosis, DNA repair and key signaling molecules of cisplatin-resistant OC cells were explored.
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PMC2196094
We have also shown that there is a very tight regulation of the different proteases in the various MC populations.
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PMC6034753
Further studies in lymphoblastoid cell lines or chickens are needed to get functional insights into MDV and CAV co-pathogenesis for T-cell transformation and persistence and dissemination of CAV.
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PMC11611077
We obtained 7 clones from the cells transfected with the expression vector including EF1α promotor and 5 clones from the cells transfected with the expression vector including CMV promotor.
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PMC11588008
Oil red O staining solution and Alizarin red staining solution were used to stain adipose induction cells and osteogenic induction cells, respectively, and uninduced well cells were used as controls.
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PMC9429973
12 pts (15%) received ≥ 1 DLI preemptively past d+120 as planned.
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PMC7823217
Printed OVCAR5 cells on Matrigel™ matrix and growth in culture medium. (
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PMC11705630
GCS cleaves glycine to produce an intermediate that later generates 5,10-methylene tetrahydrofolate (5,10-meTHF), an intermediate of one-carbon metabolism.
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PMC11185260
In accordance with these data, AURKA inhibitors (eg, alisertib) are currently being studied as a single agent for treating ATRT in clinical trials.46 Although the ATRT genome is relatively stable with a low mutational burden, in addition to the characteristic alterations of SMARCB1, DNA methylation and gene expression profiling recently identified three subgroups of ATRT, ie: ATRT-MYC (MYC oncogene-driven), ATRT-SHH (activated sonic hedgehog signaling), and ATRT-TYR (active melanoma-associated genes).14,47,48 These findings suggest that there might not be a singular molecular mechanism through which SMARCB1 universally functions as a tumor suppressor.
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PMC9250505
Transient-transfection of CAOV2 cells with siRNA directed to the BAD transcript decreased BAD expression and decreased levels of pBADS99 compared to control cells (transfected with scrambled oligo) as observed by WB analysis (Supplementary Fig. 3A).
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PMC10502403
Error bars indicate the standard deviation.
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PMC9429973
Its usefulness in patients with overt myelofibrosis (MF) is currently unknown.
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PMC9429973
Results: Hierarchical clustering of the mass spectrometry data revealed different proteomic profiles between HD and ET mutational groups and specifically between JAK2 V617F and CALR I and CALR Type II treated and/or untreated platelets.
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PMC9776316
We further dissected the mechanism underlying the role of XRCC4 in the regulation of cisplatin sensitivity.
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PMC10813895
In conclusion, a comprehensive understanding of ELF5 expression dynamics across the aging breast landscape is essential to inform targeted interventions for age-related breast cancer pathologies.
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PMC11461025
XTT Cell Viability Assay A375 cells were plated in a 100 µL volume/well in a 96-well plate at a density of 2 x 10 cells/mL. Cell density was determined using a hemocytometer.
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PMC11129910
HMECs were immortalized by transduction with LXSN CDK4 R24C, a mutant form of cyclin-dependent kinase, followed by transduction with hTERT, a catalytic subunit of the telomerase enzyme.
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PMC8409415
In the SKOV3 xenograft mice, the tumor weight was significantly lower in the cisplatin than in the PBS group (263.3 vs 503.3 mg, P < .05) (Figure 5E).
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PMC11788920
Additionally, the location of the PQS within chromatin-poor or tightly packed regions of the genome is a critical consideration.
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PMC11739696
The results from the alkaline Comet assay and qRT-PCR were analyzed with the Statistical Package for the Social Sciences (SPSS) and are reported as mean ± SD.
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PMC11543885
Mixed well before used.
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PMC11407042
The FBS concentration should not exceed 5%.
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PMC9429973
Subsequent DXA scanning revealed increased bone mineral density consistent with the diagnosis (Z -score +2.4 for lumbar spine (L1-4)) although clinical sequelae of bony disease were not apparent.
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PMC11632064
Related to Fig 2. (
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PMC11766309
This study was to investigate the prognostic value and potential immunological functions of YKT6, including cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC).
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PMC11321678
Florence et al. applied similarity network fusion (SNF) to genome‐wide DNA methylation and gene expression data from 763 MB samples, and found a very homogeneous group of patients that supported the existence of MB subtypes.
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PMC11683240
In healthy individuals, plasma concentrations of α1AT range between 0.9 and 2 mg/ml (corresponding to ∼ 17–38 μM) (47).
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PMC11574029
D, E Comparison of overall survival and disease-specific survival between high and low expression of SPP1 in the TCGA-BLCA cohort.
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PMC10362265
Panel shows the superimposed structures of human crystal complex, AF2 multimer, and docking-based predictions.
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PMC11711063
Sections were cut into slices of 5‐μm thickness, deparaffinized using xylene substitute and rehydrated using an ethanol series.
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PMC11502327
The front-end carrier-linked drug delivery system involves ligands with high adaptation to tumor cell differentiation, of which the acceptors include FA receptors, EGFR receptors, HER2 receptors, etc.,
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PMC11301242
Statistical criteria of the classification are described in detail in the tutorial file of ShatterSeek (https://github.com/parklab/ShatterSeek).
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PMC10440586
RP represents the first surgical sample taken from patients with short-term (under 5 years) recurrence, whereas NP represents patients without recurrence in 5 years. (
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PMC10073922
The findings revealed an abundant expression of NDRG2 under the transcriptional control of Sur-P in the lung cancer cell line, but not in the normal cell line, compared to the CMV promoter that enhanced NDRG2 overexpression in both normal and tumor cells.
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PMC11545737
However, S. flexneri lacking the T3SS factor OspC3 triggers caspase-4 dependent-pyroptosis in HaCaT keratinocyte and HT29 epithelial-derived cell line .
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PMC11612626
Staining protocols were performed according to the manufacturer’s instructions, and data were analyzed using FlowJo software (version 10.9.0).
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PMC11489351
To fully understand pH-dependent mechanisms driving normal human epithelial cell biology, we sought to identify the global pH-dependent transcriptome in human epithelial cells subjected to transient and physiological changes in pHi.
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PMC11572213
SYBR Green PCR Master Mix was used for the qRT-PCR reactions on the RotorGene (Qiagen) instrument.
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PMC11723210
The phases of the two planes obtained after iterative convergence are fused.
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PMC11754094
The points represent individual values. *
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PMC11461833
A previous study proposed that an antisense oligonucleotide (ASO), i.e., SRP-2001, targeted LMNA containing the p.G608G mutation to inhibit the production of progerin, promote cell growth and activate Lamin B1 expression.
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PMC11472639
Of the n = 4 MREM patients who completed all experimental procedures, n = 3 were on lenalidomide maintenance therapy following haematopoietic stem cell transplant, and n = 1 was on daratumumab maintenance therapy following haematopoietic stem cell transplant.
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PMC10788478
Lung cancer is the leading cause of cancer‐associated mortality worldwide.
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PMC11389534
Upon ligand binding, PRRs initiate diverse and interactive signalling networks that typically culminate in the expression of inflammatory cytokines or chemokines dependent on the nuclear factor‐κB (NF‐κB), as well as the expression of type I interferons (IFNs) dependent on the interferon regulatory factors (IRFs).
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PMC11615101
Graph showing the concentration of α-Taxilin in RA (n=106) compared to OA (n= 96), SLE (n=8), and HC (n=30) patients plasma samples. (
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PMC9596868
A significant increase in the expression of p53, p21 and γ-H2AX was observed in combination-treated cells, when compared to the single compound treatment indicating that the combination induces increased DNA damage in PA-1 cells (Figs. 5A and Supplementary 5A).
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PMC8389560
However, the focus of this work was the study of the Ca-dependent anticancer effect of selenium nanoparticles obtained by laser ablation in a liquid.
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PMC8010066
The resultant solution in the microtiter plate wells was then read using a plate reader, and the results were recorded (Yang et al. 2011).
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PMC11787651
Wiff files produced by the ZenoTOF 7600 were converted to mxXML using a new software we developed for this purpose (EpiConverter) and searched with an updated version of EpiProfile.
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PMC11785489
Finally, for each pair of adjacent residues within a TCR chain, we multiplied each possible combination of the five Atchley factors, resulting in 25 interactive TCR features per pair of adjacent residues.
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PMC10778532
We found no significant difference between WT VHL and VHL-C162F cells (Figure 1H).
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PMC11686380
In addition, the knockdown effects of pSuper-mGAP43#1 were not observed for GAP43R, an RNAi-resistant version of GAP43 (Fig. 3C).
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PMC10940855
At 72 hours posttreatment with shRNA-EPHB4, we observed a modest increase in BrdU incorporation in gRNA-EFNB2 multiple myeloma cells compared with shRNA-control–treated cells (Supplementary Fig. S6A).
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PMC11194678
When GFP-STAU1 was expressed at levels comparable with endogenous STAU1 (with 0.8 μg plasmid DNA), the spherical foci became bigger and brighter (Fig. 1, F and G).
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