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PMC11352311
The Cytoscape 3.9.1 software was then used to analyze and visualize the degree between targets, and the top 7 targets based on degree were identified as core targets.
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PMC11599565
Furthermore, βIII-tubulin expression was strongly stimulated by the MAP4K4i in SKOV3 cells, an effect not observed in the resistant SKOV3 subline.
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PMC11423992
RX220101; SIBP), or isotype hIgG1 (lot. #
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PMC11301095
Between 2018 and 2022, an additional 16 cases of PAM were reported worldwide, out of which 2 patients survived .
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PMC9429973
Results: A total of 20 patients were enrolled.
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PMC3122064
Therefore, the importance of cytoplasmic p21 in inhibiting Nutlin-3-induced apoptosis has been further defined using an siRNA approach.
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PMC11572213
Furthermore, the viability of TET2-downregulated HL-60 cells in low glutamine-containing medium was higher than that of control HL-60 cells.
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PMC11758416
Determine cell concentration.a.
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PMC11745823
The glioma gene expression data were downloaded by accessing the TCGA database.
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PMC11267830
The in vivo fluorescence values represented leukemia burden.
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PMC11545868
Upon ingestion of soy products, β-glucosidase hydrolyzes these isoflavone glucosides, dissolving glucose molecules and liberating the non-glycosylated isoflavone forms, daidzein, genistein, and glycitein (Figure 3) .
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PMC9874064
In conclusion, the use of USP1i combined with a platinum (IV) prodrug in nanoparticles could inhibit the growth of liver cancer.
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PMC9429973
Aims: The primary goal of the present study was the assess the effect of RTX maintenance on clinical outcomes (OS and PFS) and study its prognostic impact in patients with advanced disease.
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PMC11680982
Advanced hormone receptor (HR)-positive(+)/Human epidermal growth factor receptor 2 (HER2)-negative(-) breast cancer represents an important clinical challenge, for which the combination of cyclin-dependent kinases 4 and 6 (CDK4/6) inhibitors with endocrine therapy has emerged as the standard conventional option for both first-line and second-line treatments.
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PMC11085211
The lower concentration, 33 µg/mL, required about 6 h to show a significant increase in early apoptotic cells, inducing the increase to about 40% at 48 h (Figure 6b,c).
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PMC11742047
.
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PMC10820104
Fermentation of the fungus was carried out in ten 1L Erlenmeyer flasks, each containing 100 g of rice (Oryza Milchreis), 3.5 g of NaCl, and 110 mL of demineralized water.
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PMC9429973
Landmark OS and RFS rates (generalized gamma model) were found to be higher with Oral-AZA than MIDO (Table).
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PMC11794568
Combined with previous studies on the role of CRIP1 in cancer, the dual functions exhibited by CRIP1 in different types of cancer may be closely related to factors such as cell type, cancer stage, and tumor microenvironment.
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PMC9429973
Currently, 3 iron chelators are approved for clinical use: deferoxamine (DFO), deferiprone (DFP), and deferasirox (DFX).
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PMC7987994
This test seems to be the most appropriate method of exposure because it can be used for a very broad spectrum of types of materials of different shapes.
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PMC8751435
DNA damage will finally lead to apoptotic alternations including membrane structure damage and high permeability, which is characterized as shrinking cells.
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PMC11754094
P = 0.0483 for PE2 compared to the nCas9(H840A).
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PMC9429973
In younger patients, a second autologous hematopoietic cell transplantation (auto-HCT) remains an option in case of a prolonged remission after frontline auto-HCT.
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PMC10040136
The bottom chamber of the top chambers (Cat No. 3401, Corning, NY, USA) was used as a chemoattractant and was seeded with GIST-882 cells suspended in serum-free media.
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PMC11707832
In an in vitro study conducted by Anna Torri et al. , dendritic cells (DCs) were subjected to various compounds, revealing intriguing insights.
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PMC7642379
Top ten significant GO cellular components of the 329 common DE genes in pairwise comparisons between progeny cells and HEK293.
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PMC11332076
Since the intracellular domain of IR (iIR) plays an important role in signal transduction, we made a construct consisting of the iIR (Fig. 1A).
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PMC11509309
In order to determine whether the cytoprotective molecules 1, 2, and 5 act upstream or downstream of the induction of CHOP-mediated apoptosis, the luciferase expression level was monitored in cells treated with Tm and the bromotyrosine derivatives.
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PMC11509278
The supernatant was injected into the UHPLC–MS/MS system for component determination.
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PMC11678130
The HaCaT cells maintained over 85% viability in the case of all applied treatments, despite the observed concentration-dependent viability loss.
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PMC9917080
All presented data were obtained from at least three cell cultures from two or three different passages.
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PMC6487404
Papp (cm/s) and efflux ratio calculated for the Caco-2 cell studied at different concentrations of REG in the presence or absence of verapamil or valspodar (mean ± SD, n = 3) The influence of concentration on the transport of REG across the Caco-2 cell monolayers is demonstrated at three concentrations (20, 40 and 60 µM) (Figure 2, a and b).
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PMC11772585
We then investigated whether METTL1 deficiency itself also influenced the metabolic phenotype.
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PMC5673955
Telomerase activity determined by TRAP assay. (
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PMC10669966
PDE3A and SLFN12 protein expression were first evaluated in eight LPS cell lines (Figure 4A).
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PMC11661040
The results from three independent experiments were presented concisely in a histogram format. (
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PMC11682224
H&E staining results; (B) Tumor area statistics.
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PMC5034105
Cells were then examined under a fluorescence microscope.
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PMC10968586
Cell cycle analysis revealed that 72 h incubation of HCT116 and LoVo cells with 0.0154 mg/mL (≈100 μM) HT and 0.0231 mg/mL (≈150 μM) HT, respectively, caused G2/M cell cycle arrest and promoted apoptosis .
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PMC10093184
Brightfield pictures were taken at 10× magnification (pixel size (x/y): 0.72 µm) using a 3 h time series interval.
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PMC11089031
We designed three siRNA to KD the expression of ZNF692 and used qPCR and WB to test the effects of siRNA.
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PMC11740907
Figure 9A illustrates substantial conformational changes in inhibitor POM, adopting a complex shape post energy minimization.
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PMC9429973
A. Mechraoui, G. Ithier, J. Pages, Z. Haouari, L. Ali, A. Bonnard, M. Benkerrou, F. Missud, B. Koehl, L. Holvoet, E. Le Roux, V. Brousse Site de référence labellisé sur la drépanocytose, filière MCGRE, Service d’Hématologie-Immunologie; Unité d’épidémiologie clinique, Inserm, CIC 1426, F-75019; Service de Chirurgie, Hôpital Universitaire Robert Debré; Inserm, UMR-1123 ECEVE; Biologie Intégrée du Globule Rouge, Unité Mixte de Recherche S1134, INSERM; Laboratoire de Référence GR-Ex, Université de Paris, Paris, France Background: In children with sickle cell anemia (SCA), the spleen is altered early in life and may be the site of serious complications such as acute splenic sequestration (ASS) or hypersplenism.
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PMC7887261
Using in vitro models, Derghal et al. evaluated putative miRNA (i.e., miR-383, miR-384-3p, and miR-488) and found them physically bind to the 3′ UTR mRNA and regulate POMC expression in several neuronal subtypes.
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PMC10976516
Liu et al.Bovine herpes virusBovine herpes virus 1 (BoHV-1) strainIntratumoral with trichostatin A treatmentInfection of BoHV-1 in subcutaneous xenotransplanted A549 cell line tumor caused tumor regression.
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PMC9454178
Cell proliferation was examined using a Cell Counting Kit-8 (Yeasen, Shanghai, China) according to the instructions.
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PMC9429973
A total of 8 patients had an IWG response (BOR: CR, n=1; mCR, n=3; HI, n=4), including 6/21 SF mut patients (ORR 29%), and 2/9 SF wt patients (ORR 22%).
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PMC10968586
Pancreatic cancer, or pancreatic ductal adenocarcinoma, arises from the malignant transformation of pancreatic ductal cells, and represents the third leading cause of cancer-related death worldwide, with extremely low 5-year and overall survival rates .
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PMC2196094
No positive signals were observed with cDNA probes against either α2-microglobulin (63), TNF-α, IL-4, or IL-5.
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PMC11705484
RNA immunoprecipitation (RIP) analysis confirmed the interaction between TRMT61A protein and HMOX2 mRNA in 5637 cells.
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PMC11792090
p < 0.001, ****p < 0.0001.
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PMC9429973
Methods: A descriptive analytic study on 92 children less than 14 years of age with B-cell non-Hodgkin’s lymphoma who were admitted to Oncology Unit/ Children Welfare Teaching Hospital /Medical City campus from January 2016 to December 2018.
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PMC10040136
Prior to use, the CMs were thawed and mixed with complete culture media at a 10–20% ratio.
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PMC11036064
Thus, hsa-miR-34a-5p enhances the anti-tumoral effect of temozolomide on the cell cycle of A172 cells.
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PMC5673955
Elongation was performed for 30 min at 37°C and 10 min at 96°C for telomerase inactivation.
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PMC11767725
Therapeutic strategies for HS must address the interactions between the skin microbiome, metabolic dysfunction, and chronic inflammation.
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PMC11562028
In the hemolysis experiment, a large concentration range (50–500 μg mL) of the Re-CDs would not cause red blood cell damage in mice (Fig. 2D).
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PMC11476222
Eleven sphingolipids were identified and quantified in PSN1, as illustrated in Figure 6A.
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PMC7294030
The results were expressed as the relative density of protein normalized to β-actin.
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PMC11781181
The intracellular NAD(P)H level was measured using a NAD(P)H assay kits (Abcam, ab65348 and ab65349) according to the manufacturer's protocol.
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PMC11628904
Burn wound conversion describes the process by which the zone of stasis converts from viable to irreversibly damaged, nonviable tissue, thereby expanding the zone of coagulation in both surface area and depth. ,
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PMC7305184
Such experiments would be shorter than the one performed in this study, but sufficient enough to see, if cancer cell behavior is different form non-cancer cell.
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PMC11750165
PEX1 morpholino-injected embryos showed the increased number of GABAergic KA” neurons as well as the ectopic expression of secondary motor neurons in the p3 domain.
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PMC10335954
Western blotting was performed upon standard protocol (Sheng et al. 2018).
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PMC11453018
Data are presented as log10 fluorescence intensity.
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PMC9341513
A number of super-enhancers have previously been described in the nonvariable IGH region, including the long recognized Eα1, Eα2, and Eµ enhancers (Mills et al. 1983, 1997; Chen and Birshtein 1997).
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PMC11754784
e Real-time fluorescent kinetics of DNP2 after adding c-sgc4f and c-sgc8c.
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PMC11747885
Furthermore, our results demonstrate that Rlig1 inhibition, in conjunction with menadione-induced oxidative stress, reduces cell viability and accelerates RNA degradation, supporting the hypothesis that Rlig1 plays a critical role in the maintenance of RNA repair.
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PMC11754094
f, The effect of M4344 (ATR inhibitor) reducing large-deletion frequencies in human primary T cells, which was analysed in two healthy donors ASAN99 and ASAN107, using long-range amplicon sequencing (n = 3, mean ± s.e.m.).
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PMC8526701
As a Bcl-2 family member, p53-upregulated modulator of apoptosis (PUMA) critically functions as a regulator of apoptotic activity .
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PMC9672323
302,901) antibodies (Figures 2sa, 2sb) and cultured in the absence or presence of 7 × 10 cell line (Figures 3sa, 3sb) (E:T ratio, 10:1).
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PMC11206251
The blue-purple formazan crystal was dissolved in a shaker for 10 min.
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PMC9503541
In the second purification, F15-16 had the highest cytotoxicity activity, hence it was chosen for further purification.
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PMC11476004
Many scientists dealing with the identification of chromosomal instabilities have observed an increase in the level of both DNA and chromosome damage in old animals .
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PMC9429973
Cancer Res; 70(6): 2264–2273 A. F O Costa, L. O Marani, I. A Lopes, L. S Binelli, P. S Scheucher, J. L S Schiavinato, M. I. A Madeira, K. B B Pagnano, B. K Duarte, A. B. F Glória, E. M Rego, F. Traina, R. Welner, L. L Figueiredo-Pontes Hematology Division, Department of Medical Images, Hematology, and Clinical Oncology, Ribeirao Preto Medical School at University of Sao Paulo, Ribeirao Preto, Brazil; Division of Hematology and Oncology, Department of Medicine, UAB Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, United States of America; Hemocentro, University of Campinas, Campinas; Hematology Division, Hospital das Clinicas da Universidade Federal de Minas Gerais, Belo Horizonte; Internal Medicine, Medical School of the University of Sao Paulo, Sao Paulo, Brazil Background: Although improvements have been made in understanding the physiopathology of Acute Myeloid Leukemia (AML), most patients still relapse leading to poor long-term prognosis and cure rates.
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PMC11437637
The results with P < 0.05 were shown: Comparison of PBLs in EC patients between different age subgroups(A); Comparison of PBLs in EC patients between different BMI subgroups(B); Comparison of PBLs in EC patients between different HBP subgroups(C); Comparison of PBLs in EC patients between different DM subgroups(D); Comparison of PBLs in EC patients between different TC subgroups(E); Comparison of PBLs in EC patients between different TG subgroups(F); Comparison of PBLs in EC patients between different classification of pathology subgroups(G).
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PMC11247842
The genotype dosage is linear transformation of the posterior genotype probabilities and can take the accuracy of imputation into account when performing the GWAS.
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PMC10587429
ES only arises in humans which excludes the possibility of developing syngeneic animal models.
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PMC7039683
The efficiency of homology directed repair for this purpose remains highly locus-dependent, and we did not succeed in applying it here, although we did so at another locus (Liu et al., 2017a).
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PMC10589262
Considering its selective and high expression in GIST, BLZF1 should be further characterized as a druggable target for GIST treatment.
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PMC11763764
Finally, since SORL1 acts as an intracellular sorting receptor of many proteins and directs them to their cellular locations, the inhibition of SORL1 may cause complex effects involving multiple downstream pathways.
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PMC5261804
However, log rank p-values corrected for multiple tests using Benjamini-Yekutieli correction did not achieve statistical significance, and should therefore be interepreted with caution.
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PMC10818573
We developed three EBV detection systems: (1) a polymerase chain reaction (PCR)-based detection system, (2) a recombinase polymerase amplification (RPA)-based detection system, and (3) a combined RPA-lateral flow assay (LFA) detection system.
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PMC11473750
b The proportion of patients with chromosome 11q13.4-ter segmental copy number variation (loss [blue], gain [red]) in MYCN non-amplified and amplified patient subgroups.
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PMC9954564
Then, the remaining 1/10th of the data estimates the prediction error of the tested model.
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PMC11295144
Quantitative analysis of active synaptic contacts in which the % area was normalized to the expression level of NL2.
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PMC11742431
D–F): Flow cytometry measured the mean fluorescence intensity of KE protein internalized in 5637 cells, UMUC‐3 cells, and A375 cells.
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PMC11401358
Notably, SF showed the strongest ability to scavenge H2O2, while its ability to scavenge •OH and O2−• was relatively weak among the nine biomaterials (Fig. 2A and Fig. S1).Fig.
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PMC10033453
TCID50 values were determined by the Spearman-Kärber algorithm using serial viral dilutions in BHK-21 cells.
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PMC9429973
We excluded patients who died within 100 days of ASCT.
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PMC9429973
The ultimate goal would be to detect carrier couples with a premarital screening program; however, it has been challenging to implement thus far.
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PMC9844987
DeepLIFT and TF-MoDISco were run independently on the input sequences for low variable and highly variable promoters.
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PMC6181431
Some were known cancer genes, including CDKN2A, KEAP1, MYCN, PLK4, SETD2, TP53.
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PMC10092581
The cytotoxicity was assessed by the MTT test.
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PMC11040965
Data from GSE4581 (left) and GSE9782 (right).
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PMC9739791
The complex 17 was also found to be able to generate singlet oxygen in the experiment with singlet oxygen trap 1,3-diphenylisobenzofuran (DPBF), although the efficiency of the O2 production was lower than that of the bodipy ligand.
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PMC10344560
It is widespread and commonly found in air, ocean water, lakes, soil, fruit juice, and milk.
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PMC8345486
Activation of p53 and its phosphorylation at Ser15 and Ser37 and acetylation play a positive role in the accumulation of p53 protein in stress response, which leads to apoptosis and inhibition of the cell cycle, which is true for wt p53.
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PMC11589157
After filtering adaptors and low-quality reads (when the proportion of low-quality bases exceeded 20% of the read length), we obtained 1,011,168,632 paired-end clean reads for further genome assembly.
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