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PMC11607638
SKR‐CHO‐K1 cells were more responsive to SK2 than SK1, with EC50 of 251 pM (SK2) and 1.18 nM (SK1), respectively (Figs. 4, 5).
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PMC9429973
Aims: We determined if adding another anti-myeloma drug to Len would lead to improved outcomes in patients with MM following autoHCT.
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PMC11697189
not significant.
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PMC11794847
A: The silver staining image of IP-MS with HVEM protein in Jurkat T cells; B: COIP assay indicated that HVEM could interact with FOXA2; C: Immunofluorescence staining confirmed the co-localization of HVEM and FOXA2 in the cytoplasm; D: Molecular docking revealed the interaction between HVEM and FOXA2; E: Dual-luciferase activity analysis showed Si-FOXA2 could increase the luciferase activity of GPT2-WT but not that of GPT2-MUT; F: GPT2 mRNA was upregulated after FOXA2 knockdown; G: HVEM knockdown had no infouence on the mRNA expression level of FOXA2;H: Nuclear-cytoplasmic fractionation assay results HVEM regulates GPT2 by interacting with its upstream transcription factor FOXA2.
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PMC11755467
The DNA template was purified using a PCR clean-up kit (Qiagen, Minden, Germany).
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PMC11638255
To examine changes in the immune environment, we performed OPAL multiplex immunohistochemistry on tumors obtained after 14 days of treatment (Fig. 1d).
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PMC9429973
Further, the VS results outlined their similar or even higher affinity to Pgp compared to Dasatinib, which was used as reference compound due to its dual BCR-ABL and SRC inhibitory activity.
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PMC9429973
Aims: To set up a model of cooperation between TLX3 and FLT3-ITD and to elucidate the oncogenic role of TLX3 in T-ALL.
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PMC6102174
Wogonin could inhibit HBsAg and HBeAg with an IC50 value of 4 μg/mL, and DHBV DNA polymerase with an IC50 value of 0.57 μg/mL .
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PMC10743717
Unfortunately, such an ABAD/ERAB experimental procedure was found to be nonsense for biomedical research at all (see Figure 2 of Ref. ).
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PMC11279397
Additionally, treatment with PA inhibited cytokine expression and RLH pathway mRNA levels associated with H1N1 influenza virus infection in vitro .
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PMC10253553
De-identified frozen patient renal tissue samples were obtained from the biorepository bank at the Edwards Comprehensive Cancer Center at Cabell Huntington Hospital, Huntington, WV.
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PMC11723947
For transcription factor expression analysis, cells were labeled with PE-conjugated anti-T-Bet and APC-coupled anti-Foxp3 (236 A/E7, Thermo Fisher Scientific) antibodies using the Transcription Factor Staining Buffer Set (Thermo Fisher Scientific).
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PMC11703896
Rapamycin in the extracted sample was confirmed by comparing the standard rapamycin peak (Rani et al. 2013).
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PMC9429973
Summary/Conclusion: Evaluation of level of protein S and C in COVID-19 patient is very important at the admission moment in ICU.
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PMC11190538
The yoghurt samples were preserved in the refrigerator and lactic acid bacteria are normally psychotropic and can grow at refrigerator temperature.
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PMC7602170
This review highlights the progress for PI3K/AKT/mTOR-dependent mechanisms in GISTs, and explores the relationship between mTOR downstream signals, in particular, eukaryotic initiation factors (eIFs) and the development of GISTs, which may be instrumental for identifying novel therapeutic targets.
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PMC11190538
Fig. 5Transmission electron microscopy of C-PC loaded nanophytosomesC-PC: Phytosome (Molar Ratio) 1: 1, 1: 2, and 1:3.Fig.
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PMC11185260
Figure 7scL-SMARCB1 nanocomplex promotes radiation-induced cytotoxicity in ATRT.
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PMC10874773
They are distributed in plants such as I. merrillianum and I. jiadifengpi.
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PMC11591794
The viability rate in HaCaT cells was observed as 96.35 in the 24-h application, 94.20 in the 48-h application, and 84.03 in the 72-h application.
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PMC11711935
Fibroblast‐derived clusterin (CLU) negatively affects cutaneous pigmentation .
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PMC8143558
Urb.,
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PMC7039683
Coordinates for five genomic regions with overlapped TP63, IRF6, KLF4 and H3K27Ac ChIP-seq peaks. (
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PMC3681794
Data are representative of 3 independent experiments.
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PMC10578720
Following 10 weeks of DCA treatment, all mice were anesthetized with isoflurane and sacrificed by cervical dislocation; the liver and spleen tissues were harvested for subsequent assays.
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PMC11766332
To explore the potential functional mechanisms of ASD-miRNAs, we constructed a regulatory network based on the nine miRNAs analyzed in this study.
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PMC11708541
Molecular docking and density functional theory were used in kinetic experiments to examine the type of contacts, free energy, and manner of inhibition (Scheme 8).
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PMC3813807
This supports the hypothesis that miR-373 induces the proliferation of gastric cancer cell lines by negatively regulating the expression of TNFAIP1 at the protein level.
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PMC10506676
Statistical significance was defined as a P-value less than 0.05.
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PMC9429973
Studies were also mainly conducted in Europe, being particularly scarce in Asia.
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PMC9429973
In the group of BSC, at a median of follow-up of 24 days (3 to 894), the median OS was 22 days (range 3 to 541); in non-intensive treatment, at a median of follow up of 165 days (range 28 to 943), the median OS was 165 days (range 60 to 943) (p<0.0001).
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PMC9429973
Aims: To explore the mechanism of G-CSF on NK cells.
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PMC8751435
CME‐induced pyroptosis and GSDME regulation are validated to be caspase‐3 dependent in loss‐of‐function assay, which may provide a novel insight for understanding anticancer property of CME.
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PMC10362265
Thus, these data solely relying on the static models fail to address whether and how the short surface epitope can affect heterodimer stability and self-assembly.
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PMC11391695
GAPDH (Absin, China), ERK and p-ERK (Proteintech, China), P38, p-P38, JNK and p-JNK (Abmart, China), ACYP2, KCNN4, and TERT (Abcam, UK) were the primary antibodies employed in this investigation.
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PMC7812570
Target cells stably expressing GPI-m36.4 or GPI-FluIgG03 (scFv) were generated as described previously.
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PMC11380454
In contrast, PD-1 did not induce increased proliferation in VLA-4-negative B16/F10 cells, most likely because of PD-L1 deficiency (Fig. 8F).
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PMC9429973
A median of 3 cycles (range 1-11) were given.
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PMC8358006
Conversely, 208 of the 557 genes (37.3%) that showed less H3K27me3 are highly expression in KYSE450 cells (Fig. 3c, Supplementary Table 3).
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PMC10706275
This study provides a viable approach to establishing a CHO cell line capable of enduring reliable exogenous protein expression.
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PMC11267830
Survival curves were compared using the log-rank test.
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PMC10033453
B–E) Tumor infiltrating lymphocyte (TIL) subsets were compared between VMG and PBS control mice by flow cytometry.
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PMC10998613
MDA-MB-231 cells were culture in serum starved overnight (16 h) and applied to pretreatment with 10 µM of AMD3100 (anti-CXCR4) and ICI-118,551 (anti- β2AR) as indicated before treatment of CXCL12 and epinephrine respectively.
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PMC6450504
The Z-projection was obtained using maximum pixel intensity collected from each layer of the spheroid.
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PMC9429973
Mounting evidence shows that the GM of patients with high rates of infection are characterized by an imbalance of bacteria, inducing proinflammatory states and reduced capacity for SCFA synthesis.
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PMC10490530
These results indicated that TNFα acts as a positive regulator, but its regulatory effect on the PIK3CA promoter is compromised when p53 is over-expressed and stabilized.
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PMC11718109
When co-expressed together with mCherry-synapsin, VMAT2 co-assembled with synapsin into droplet-like condensates (Fig. 5A, B, Supplementary Fig. 5B, C) reminiscent of those generated by synaptophysin and synapsin (Fig. 5C, D).
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PMC10578720
In this respect, we demonstrated that HBV inhibits SIRT3 expression in macrophages by means of the TLR2-NF-κB-PGC-1α axis, resulting in hyperacetylation of CS/PDHC and impairment of the TCA cycle, contributing to M2-like polarization.
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PMC11806182
Testosterone was found to up-regulate cholesterol metabolism while down-regulating estrogen 16-alpha-hydroxylase activity, reflecting its complex role in UCEC.
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PMC11683240
Notably, when residue N314 is included, the destabilization is higher.
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PMC11607321
While further experimental validation is necessary, this could pave the way for the identification of cancer cellular states underlying drug resistance.
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PMC11486946
By observing individual cells under uniform fluorescence excitation at high magnification, quantitative analysis of the position as well as the fluorescence intensity of the fluorescent spots is possible, which allows the characterization of not only the lateral mobility of the target molecule but also the cluster formation of the target molecule.
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PMC9429973
We hypothesize that TET2 acts as a tumor suppressor in T-ALL, involved in a premalignant clonally expanded pre-LSC population.
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PMC11604768
N.D., no drug; NT, neurotensin 8−13 [100 nM]; NT + FR, neurotensin 8−13 [100 nM] + FR900359 [50 nM].
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PMC11781181
Initially, we revealed that keratinocyte internalized TEN Exo loaded with miR-375-3p (Fig. S10A).
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PMC9429973
Retreatment with previous agents were the SoC following TCE.
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PMC11733919
Among the different methodologies that can be used to orient the Ab on the MNP, we selected specific adaptor molecules.
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PMC8658661
These genes are also overexpressed in HB , but in the case of HCC, a change in expression has only been shown for IGF2BP1, an increased value of which is associated with a poor prognosis for the course of the disease .
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PMC9429973
Calr VAVCre mice and chimeric transplanted Calr mice were treated with a proteasome inhibitor, an IRE1a inhibitor, or both.
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PMC11705630
These results collectively suggest that inhibition of GLDC depletes dNTP cellular levels and the depleted dNTP levels in turn induce mtROS productions in RCC cells.
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PMC11188874
Pan-cancer cell effect of HDAC6 inhibitors on proteasomes and MHC class I antigen presentation.
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PMC11670954
BA is a vital short-chain fatty acid that supplies the majority of the energy required by intestinal mucosal epithelial cells.
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PMC9545714
The present results from Figure 1 show that after 16 weeks of GTE supplementation the TEWL subjects have TEWL values lower than the placebo controls.
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PMC10669966
PDE3A and SLFN12 mRNA levels were measured from 63 randomly selected FFPE LPS samples, excluding WDLPS samples.
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PMC9844987
To assess in general how individual decomposed promoters influence the overall promoter variability, we calculated the expression-adjusted dispersion (adjusted log10-transformed CV) of local-maxima decomposed promoters.
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PMC11718817
It was found that many effects on T cell motility mediated by nigericin-treated macrophages (such as average speed, but also track displacement and track length) were preserved in IL-18R-deficient T cells.
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PMC11258145
It may form oligomers spanning opposite bilayer leaflets.
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PMC9429973
Methods: The study comprises at least 7 dose escalating cohorts.
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PMC11731614
Thus, Notch signaling may represent an unexplored, novel communication pathway between keratinocytes and DRG neuron terminal nerve endings.
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PMC11686380
Variant annotations were performed by the Variant Interpreter (Illumina, San Diego, USA).
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PMC11543885
Determine the spatial specificity of the NLRP3-APEX2 induced biotinylation via the confocal immunofluorescence results.
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PMC8414691
Scale bar = 5 μm.
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PMC3675084
OVCAR-3 cells were maintained in RPMI-1640 medium (Irvine Scientific, Santa Ana, CA) supplemented with 10% fetal bovine serum (FBS; Gemini Bio-Products, Inc., Calabasas, CA); OVCAR-3-TR were maintained in the same medium containing 0.5 µM of paclitaxel.
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PMC9429973
SF3B1nent patients showed on average more mutations (MDS with isolated del(5q): 1.9; MDS-EB: 2.7; MDS-RS: 3.1).
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PMC8540611
The cells were incubated for 3 or 24 h and washed in PBS according to the standard procedure.
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PMC10758402
HSV titers were determined by a standard plaque assay on Vero cells according to a previously published protocol (13).
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PMC6267596
Most of the data analyses were done by Perseus as per their recommended method .
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PMC11666785
Multicolor variant such as multicolor STORM facilitated imaging of large volumes with ultrathin sectioning of ganglion cells to understand the nanoscale co-organization of AMPA receptors and their spatial correlation.
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PMC11703992
D The MR estimates of significant exposures on GCA in validation dataset.
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PMC11066331
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a widely used analytical technique for identification, structural analysis, and quantification of various chemical species, including those constituting complex biological samples.
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PMC9429973
HR and Risk-Ratios with 95% CI in Table.
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PMC11476264
Moreover, ZEB1 knockdown alone showed the lowest cellular mobility and aggressiveness.
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PMC11345828
The crude residue was purified by flash column chromatography eluting with 0 to 5% MeOH in DCM to afford 44 (5.2 g, 84% yield).
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PMC10452486
Within 2–4 weeks after transfection, most of the male mice possessed B2m-free B27 HCs (HLA-B27-Face-2), but not with B2m-associated HLA-B27 (B27-Face-1).
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PMC10170482
Moreover, MitoTracker red CM-H2XRos was shown to have high mitochondrial specificity and high sensitivity for mitochondrial ROS detection.
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PMC9429973
Also, the chidamide alone or combined treatment significantly induced the G0/G1 phase arrest in the cells (Fig. 1C).
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PMC11352877
The same trend was observed with the SK-UT-1 cell line compared to A2780 (p < 0.0001) and RL-95-2 cells (p < 0.0001) (Figure 3B, Table 3).
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PMC10507284
Meanwhile, previous studies have proved that TROAP is an essential protein for the function of trophin as a cell adhesion molecule and is relevant to human embryo implantation and endometrial formation.
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PMC11679033
Treatment with single CBD resulted in decreased CBPI and RI values compared to the negative/solvent controls, owing to increased proportions of M2, and decreased proportions of M3 and M4 cells.
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PMC6364197
Flow cytometry analysis was performed using FC500 (Beckman Coulter, Inc., Brea, CA, USA).
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PMC11422497
KIT was simultaneously predicted by three miRNA databases (miRBase, TargetScan, and miRanda). (
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PMC11509278
Skin is an androgen target tissue in which 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and 5α-reductase convert dehydroepiandrosterone, androstenedione, and testosterone into the most effective natural androgen dihydrotestosterone (DHT).
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PMC7759933
At both initial densities, the spheroids maintained uniform spherical shape over the course of the culturing.
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PMC8414691
As shown in Fig. 6C, Cit-K, MKLP2, Aurora B, PLK1, CEP55, Anillin, Alix, and Spastin, together with α-Tubulin, were found in the IP sample immunoprecipitated with SRCAP antibody, but not in the negative control.
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PMC11345828
E. Comparison of relative tumor volumes for each dosing cohort.
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PMC7759933
The basal mRNA level of CYP1A1 was approximately 2-fold higher than in monolayer culture and remained at the same level throughout the whole duration of the spheroid cultivation.
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PMC9429973
Summary/Conclusion: These preliminary data confirm our previous report and suggest the proliferating fraction and the incidences of tumor-associated myeloid cells and T cells as flow cytometry-identifiable biomarkers that could assist in the differentiation between aggressive vs. indolent B-NHLs in specimens collected via minimally invasive sampling.
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PMC8740518
Tumors were collected and preserved in 4% paraformaldehyde.
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PMC9429973
The outcome was excellent with complete remission of his SM component & marked clinical improvement.
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