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PMC11490153
MDA-MB-231 cells showed the most sensitivity to citral-AlgNP at hypoxic conditions.
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PMC11578343
D eff is determined empirically for each device design by calibrating the free cell diameters measured with the device to those obtained by a commercial cell counter (Millipore Scepter 2.0).
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PMC11721064
Furthermore, P3 LNCs were single-stained for Oct4 and SOX2 (scale bar = 50 μm).
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PMC10506676
The CtNorm algorithm, which can be accessed online at http://ctnorm.sums.ac.ir, was used to equalize the Ct values of the qRT-PCR runs (25).
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PMC11707577
Cell viability was evaluated by using CellTiter‐Glo® 3D Cell Viability Assay (Promega).
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PMC11507371
This indicates that only a slight improvement in redox adaptation is feasible, as the adaptation capacity seems to be depleted.
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PMC10158546
We report that the differentiated cultures of ReN cells were resistant to infection with multiple prion isolates despite a high level of PrP expression mediated through lentivirus transduction.
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PMC5417661
The bone metastatic breast adenocarcinoma cell line MDA-MB-231-SA (Dulbecco’s Modified Eagle’s Medium [DMEM]), the nontumorigenic breast luminal epithelial cell line MCF-10A (DMEM:HAM’s F12, 1:1), the human intestinal Caco-2 cell line (EMEM), and the ovarian adenocarcinoma cell lines OvCar-8 (RPMI 1640), OvCar-4 (RPMI 1640) and CaOv-3 (DMEM) were purchased from the American Type Culture Collection (Manassas, VA, USA).
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PMC11078693
Keep the remaining adherent cells in the T75 flask in 5 mL of 1× PBS to rinse away the dye and prevent drying while the suspension is spinning.
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PMC11201245
The BAC DNA was isolated using a Qiagen Plasmid Mini Kit (Qiagen, Hilden, Germany).
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PMC9429973
Summary/Conclusion: Taken together, our findings indicate that the aberrant overexpression of ERG maintains HSPCs in an undifferentiated state and promotes AML development.
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PMC11656048
These findings indicate that shikonin can induce iron death in OS cells.
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PMC4360730
The ‘Biosample treatment’ facet displays the term names for treatments, some of which are annotated to ChEBI.
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PMC10849234
Measurement of primary granule exocytosis by increased CD63 membrane expression in differentiated PLB-985 cells expressing dCas9-KRAB (control) compared to PLB-985 cells expressing dCas9-KRAB and Rab27a sgRNA. (
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PMC9429973
53 (76%) pts had symptomatic COVID syndrome and 43 (61%) were hospitalized, with no differences between the two groups.
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PMC11604830
CSI images acquired before the injection of the nanoparticles demonstrated the absence of any signal at the target frequency (3.7 ppm) (Figure 3B,C), while 30 min after the injection of the nanoemulsion, a clear signal was detected in the tumor rim, both by classical T2 weighted MRI (Figure 3D), which reports about the liquid accumulation, and by H‐CSI (Figure 3E,F).
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PMC11792766
The highest concentration at which ethanol and acetic acid did not exert adverse effects was determined using NOAEC values.
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PMC7582629
Additionally, the combination of RS with microfluidic devices and high-throughput screening for improving the velocity and the number of cells analyzed are also discussed.
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PMC11438317
Proteins were transferred to nitrocellulose membranes after SDS-PAGE, and the presence of potential substrates in the precipitates was detected by Western Blot.
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PMC11627060
Present isolated TQ, TQ-BSA NPs, and Cisplatin significantly reduced cell viability as compared to control at 100% cell viability (p < 0.05) and significant difference from the concentration using the DMRT statistic (p < 0.05).
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PMC11678841
Based on these results, in subsequent experiments, we focused on the potential immunomodulatory activity of GA.
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PMC11529598
Previous studies have shown that miRNAs play an important role in glioblastoma biological processes.
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PMC11574271
Our analysis revealed differential expression patterns between these two cell lines, with MMP12 identified as a downstream target of the RON signaling that is directly associated with invasive and migratory properties (Fig. 2).
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PMC8524093
In addition to the classical sylvatic and urban transmission cycles, CHIKV infection can also occur by vertical transmission during pregnancy and blood transfusion.
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PMC7342409
All experiments were performed following the Hangzhou cancer hospital and national guidelines and regulations.
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PMC10607604
Therefore, here, we silenced PTPN13 in the KURAMOCHI cell line (high PTPN13 expression and low aggressivity) and overexpressed PTPN13 in the OVCAR-8 cell line (weak PTPN13 expression and higher aggressivity).
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PMC11224020
Data were analyzed by Kruskal–Wallis test; ****P < 0.0001.
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PMC11756907
These results indicate that the attachment of 2-ME to the GO/rGO graphene nanoflakes in most of the tested systems provides greater cytotoxicity of the biocomposite compared to pure graphene nanoflakes.
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PMC11514418
Kaplan-Meier curves of OS were plotted, and Log-rank tests were also conducted.
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PMC11537649
CDK6 belongs to the cdc2 related kinase family, with silk/threonine kinase activity, and is a key regulatory factor of the cell cycle in mammalian cells.
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PMC11658074
The mechanism by which AT-EVs are taken up more rapidly by cells is due to the expression of CD151 on the surface of AT-EVs.
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PMC11612315
Effects of intraperitoneal (b) and oral (c) administration of 10 μg/kg GLP-1(7–36) (GLP-1) on heat sensitivity via the hot plate test (mean ± S.E.M., n = 5 ~ 7).
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PMC11335267
CTX, cortex; HIP, hippocampus; STR, striatum; THL, thalamus; HYP, hypothalamus; MdB, midbrain; CER, cerebellum. **
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PMC9429973
Forty (61%) pts were ≥65 years old with a median age of 73 (range, 65-85); 18 pts were ≥75 years old.
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PMC9118379
This resulted in the peptide sequences in RefP_V yielded by de novo sequencing tools, which were used to examine the interpretation consistency between sequence database searches and de novo peptide sequencing.
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PMC9119314
Upon termination of the assay, the plates were stored at −20°C overnight.
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PMC11496728
In our future studies, we can express some difficult-to-express proteins with the established novel CHO cell expression system, which lays the foundation for the large-scale production of recombinant proteins.
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PMC11477621
The crude product was purified by silica gel chromatography to give the colorless oil 2e (300 mg, 85%).
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PMC11718031
Glucose was the primary energy source for all media used in this basal media screening study.
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PMC11806106
D Averaged FRET-based single-cell recording of the MOR conformation sensor with sYFP inserted after G267 and mTurq2 inserted after T370.
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PMC11754094
c, Data from CRISPRi screening with Nanopore-seq plotted as Z-scores of the frequency of large-deletion events in cells with the indicated gene knocked down.
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PMC11461874
The expression of firefly luciferase was driven by three tandem D-box (TTACGTAA) sequences.
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PMC11684821
TCR sequencing of MR1-restricted cancer-activated T cells showed that the majority of these cells shared a conserved 10-amino acid–long TCR-α chain motif as a result of TRAJ42 use.
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PMC8389560
It is known that cisplatin is used in treatment of solid tumors and modulates the release of [Ca]i from ER.
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PMC10728200
We evaluated the potential tumor suppressive effect of RSL3 in combination with IM in GIST-T1 and GIST-882 cells by treating with different concentrations of drug combinations.
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PMC9429973
Methods: A national real-world study of ICH in adult ITP patients was conducted using data from 27 centres.
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PMC11696576
Water was then added (5 mL), the reaction mixture was stirred for 30 more minutes and filtrated over Celite.
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PMC6450504
This shift could be attributed to the adsorption of albumin and other serum proteins, which are negatively charged at pH 7.4 (Milošev, 2012) onto the liposome surface.
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PMC11701801
SMARCA4 also localized to the SS18-localized regions at the KREMEN2 locus (Fig. 3D); however, ATAC-seq signals near the TSS of the KREMEN2 locus in Aska-SS shSSX (with SS18-WT) cells were more attenuated than that in Aska-SS shNT (harboring SS18–SSX) cells (Fig. 3D).
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PMC10203589
In the treatment of cervical cancer.
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PMC6024716
Comparison of total and specific luciferase activity from the OAZ1 knockout and the parental cells are shown in Figure 3a,b. Specific luciferase activity was not affected by the cell growth and, as expected, there was correlation between total activity and cell density.
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PMC11585254
Data for panel B were analyzed by one-way ANOVA, followed by Dunnett’s multiple comparisons test, and the adjusted P values are shown.
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PMC9429973
Confirmatory experiments were completed in multiple T-ALL preclinical models.
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PMC11626627
The predicted impact of ERβ (ESR2) and TWIST1 on the survival of kidney cancer patients in the TCGA-KIRC database was analyzed.
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PMC11143482
Originally, humanised mice were defined as mice with functional human genes, cells, or tissues.
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PMC9118379
By inspecting the existence of wild-type counterpart peptides and parental proteins, we found that the 98 variant events in the 320 variant PSMs reflected different levels of reliability.
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PMC10745221
A methionine-restricted diet in Phase I clinical trials as well as a Phase I study of methioninase with cancer patients have been shown to reduce plasma methionine levels without major complications .
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PMC11682525
The enhanced viability of SH-SY5Y cells and hHFSCs under oxidative stress conditions indicates the potential for improved therapeutic outcomes in central nervous system cell therapy.
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PMC11476106
Moreover, mechanotransduction also induces feedback mechanisms that reciprocally influence ECM composition and architecture.
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PMC9351137
HeLa cells (ATTC company) were cultured in 6-well plates in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (Corning) and a penicillin/streptomycin solution (Gibco, 15140122).
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PMC9429973
At 6 months 28% and 50% of ptn with suboptimal and failure responses, respectively, switched their TKI.
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PMC11707832
Lysosomal α-mannosidase, encoded by the MAN2B1 (LAMAN) gene, belongs to the α-mannosidase II family, catalysing the hydrolysis of terminal non-reducing α-D-mannose residues within α-D-mannosides during the ordered degradation of N-linked glycans .
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PMC10380508
The current treatment modalities for RCC mainly include surgery, chemotherapy, immunotherapy, and molecular targeted therapy.
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PMC11653168
This observation is distinct from findings in some previous studies (24,25), which reported more rapid inhibition of STAT3 phosphorylation.
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PMC11366496
oeRNF122 promotes glioblastoma cell growth. (
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PMC5833712
In contrast, the ERβ-selective agonist, DPN, significantly suppressed the growth of the two ovarian cancer cells.
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PMC11753435
Additional parameters used for data filtration can be found in (28).
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PMC11596967
Recent advancements in large-scale transcriptome analyses have effectively characterized lmRNAs in bacteria and archaea, which can initiate translation directly at TSSs .
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PMC11806106
Morphine hydrochloride was purchased from Merck (Darmstadt, Germany), naloxone-HCl and dynorphin A TFA salt were purchased from Cayman Chemical (Ann Arbor, Michigan, USA), l-methadone-HCl was purchased from Hoechst AG (Frankfurt, Germany), nociceptin (1–13) amide TFA was purchased from TargetMol (Boston, MA, USA) and JTC-801 was purchased from Tocris Bioscience (Bristol, United Kingdom).
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PMC9429973
Methods: This ambilinear observational study included patients with CLL with high genetic complexity (high-CK), defined as >=5 aberrations or CK (>=3 aberrations) in combination with a 17p deletion (CK+del17p).
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PMC11026382
The dUMP substrate is in green sticks.
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PMC11502327
Ovarian cancer (OC) is one of the deadliest gynecological malignancies in the world and is the leading cause of cancer-related death in women.
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PMC11786767
Arrows indicate the biotin-DHPE signals located in the nucleolus during interphase.
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PMC6948907
Therefore, we decided to investigate the effects of these compounds (alone and combined) on proliferation and apoptosis of hepatocellular carcinoma HepG 2 cell line.
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PMC10093184
This observation correlates well with the RT-qPCR measured increase in miR-9 and decrease in miR-34a levels in these cells, as overexpression of miR-9 promotes and overexpression of miR-34a inhibits osteosarcoma cell migration .
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PMC11337594
The error bars represent the means ± SDs (n = 3). (
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PMC5916734
A specific SQ-fVQ-R oligonucleotide was used to search for the regions of altered DNA by colony PCR.
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PMC11754432
Slides were incubated with polyclonal Goat anti-Rabbit Immunoglobulins/HRP (1:500; P0448, DAKO, Agilent, Santa Clara, California, USA) for 10 min.
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PMC9429973
Thus, better understanding of the disease and more innovative therapeutic approaches are needed.
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PMC11412721
Means ± SEM (n = 6 mice per assay and genotype).
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PMC11381192
Cumulative distribution function curves displaying different clustering approaches for k values ranging from 2 to 9. (
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PMC9429973
CRS occurred less frequently in pts aged ≥65 years than in those aged <65 years (30% vs 52%), while CRS events were predominantly low-Gr in both groups (age ≥65 vs <65 years: Gr 1, 20% vs 28%; Gr 2, 7% vs 22%); high-Gr CRS was uncommon (one patient in each group).
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PMC11453018
The strongest synergistic effects were observed with Dasatinib, while other senolytics demonstrated varying levels of synergy or antagonism.
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PMC9429973
Image: Summary/Conclusion: Our study showed that real-world experience of treating patients with R/R AML with venetoclax in combination with hypomethylating agents or low-dose cytarabine is feasible as salvage treatment in patients relapsed to hypomethylating agent and as bridge therapy to alloSCT with a rapid response rate.
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PMC10142392
As previously discussed, the branched or multi-arm architectures offer interesting properties to enhance the effectiveness of polymeric nanocarriers as drug delivery vehicles.
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PMC11377827
P = 0.0006, two-way ANOVA. (
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PMC11490153
The diluted EO sample (1 µL in n-hexane) was injected into the GC/MS system.
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PMC6835428
From one point of view, this paper attenuated the enthusiasm for targeted delivery systems.
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PMC11609529
Digital images were captured using a Leica DM6000 B microscope and LAS X software.
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PMC11496728
Comparison of transient and stable expression levels and relative copy number of SEAP in CHO-WT and CHO-KO cells.
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PMC11578343
Heatmap of the measured viscous G″ modulus for each single cell in each zone, grouped by cell type.
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PMC6160470
Based on available research, we hypothesized that 18-crown-6 ethers might show inhibitory activity towards P-gp, in addition to inhibiting tumour cell growth.
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PMC11696659
Receiver operating characteristic (ROC) curves are shown to compare performances of the validation cohorts at LC prevalences of 48 % in TAV model 1 (A) and of 5 % in TAV model 2 (B).AUC, area under the curve.
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PMC11388384
In cell culture, morphogens are degraded primarily through loss to the open tissue culture media or through receptor/coreceptor-mediated degradation.
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PMC11756514
WTA and LTA of S. pneumoniae have identical chains with 4 to 8 repeating units of AATGalNAc, glucose, ribitol-phosphate, and two GalNAc (45).
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PMC9429973
The median Hemoglobin and platelet counts were slightly higher in CALR positive group (12.3 g/dL and 10.15x10/l) compared to JAK2 mutated and triple negative groups but the difference was not statistically significant.
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PMC9920575
After 2 h at 37 °C, the absorbance was read at 490 nm using a microplate spectrophotometer (Thermo Scientific, Multiskan GO, Madrid, Spain).
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PMC7612475
However, in the context of this study, this may reflect a difference in the importance of replication stress to drive tumorigenesis or the differential dependencies on HR to maintain genome stability and cell viability following treatment between different cell types.
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PMC10976516
Frentzen et al.EphA2-TEA-VV, a T cell engager armed oncolytic VV (TEA-VV) encoding secretory bispecific.
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PMC10999934
A study found that reducing PLXNB2 levels can inhibit the proliferation and invasion of ovarian cancer cell .
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