PMCID
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PMC11721064
This algorithm infers co-expression regulatory networks between TFs and candidate target genes from scRNA-seq datasets.
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PMC4485648
Dual-luciferase results were calculated for each transfectant and analyzed by the data analysis software (SABioscience).
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PMC11472639
All conditions were seeded in triplicate wells and relative fluorescent units were converted to a percentage of specific lysis using the following equation:%SpecificLysis=((Sample–Spontaneous)/(TritonX−100–Spontaneous))x100 Sodium heparin-treated whole blood (300 μL for tubes used to identify plasma cells, and 100 μL for tubes used to identify monocytes and T-cells) were labelled with surface staining antibody-fluorochrome cocktails for 30-min (discussed later) at room temperature and subsequently treated with 1 × FACS Lysing Solution (BD Biosciences, Wokingham, UK) for 10-min at room temperature.
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PMC11577421
Conversely, in pgsA-745 cells, the difference in uptake levels between NP_BSA+ and NP_BSA was much less pronounced.
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PMC8633974
Human embryonic kidney cells (HEK293FT) cells were grown in Dulbecco's Modified Eagle's Media (DMEM) containing 10% v/v fetal bovine serum (FBS), 100 units/mL penicillin and streptomycin (PenStrep), 0.5 mg/mL Geneticin®, 1% v/v MEM non-essential amino acids (MEM NEAA), and 1 mM sodium pyruvate.
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PMC11680562
The Mueller–Hinton agar solutions were initially autoclaved at 121 °C and 15 PSI for 15 min.
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PMC10452486
They are upregulated on immune cells upon activation by proinflammatory cytokines, anti-CD3 antibodies, antibiotics (e.g., ionomycin), phytohemagglutinin, retinoic acid, and phorbol myristate acetate.
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PMC11788920
We designed DNA constructs in which the PQS and dCas9 target sites were placed downstream of the TSS in order to probe their propensity to stall T7 RNAP progression .
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PMC11803149
The volcano plot shows DEGs between OT-I T cells with Epas1-Crispr and control-Crispr (log2 fold change > 0.5 or < -0.5 and adjusted p < 0.05).
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PMC11604768
Orange and gray projection lines indicate the positive and negative directions of the treatment projection, respectively.
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PMC8540611
However, this is lower than for HeLa cells and amounts to 16.5 ± 3.1% and 25.7 ± 1.3% for HL-60 and CEM-SS cells, respectively.
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PMC11653533
Belonging to the class Magnoliopsida and subclass Dilleniidae, it is classified under Urticales and the family Moraceae, demonstrating its close evolutionary connection to nettles and mulberries.
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PMC9429973
Adverse events (AEs) were monitored throughout the study.
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PMC8839885
Results demonstrated that co-culture caused a significant reduction in the level of cleaved PARP as expected (Figure 3).
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PMC11705547
Cisplatin can reverse the immunosuppressive conditions in the tumor microenvironment by promoting M1 polarization of the TAMs .
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PMC11790971
For the experiments, CHO cells were thawed and cultured at 5% CO2 at 37 °C in a shaking incubator rotating at 140 rpm.
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PMC10652261
Based on the data, compounds 2e, 2f, and 2h caused caspase 3 induction more than bortezomib.
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PMC11696659
A summary list of TAV is presented in Supplementary Table 1.
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PMC10813895
The primary goal of cancer prevention trials is to reduce cancer incidence, which can be difficult to measure due to the long timespans required to do so.
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PMC11758416
The number of L cells for a 10:1 (Effector:Target) ratio would then be 5E3 L cells per well.b.
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PMC11483468
The purified recombinant protein GST-TAT-LPTS39 entered the BEL-7404 cell nucleus, as detected by Western blot.
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PMC11754436
At 1 month after the injection, the nude mice were sacrificed.
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PMC9429973
N. Aggarwal, A. L. Manley, J. Durrani, R. Shalhoub, O. Rios, J. Lotter, B. Patel, C. Wu, N. Young, E. Groarke Hematology Branch; Office of Biostatistics Research, National Heart, Lung, and Blood Institute, Bethesda, United States of America Background: Aplastic anemia (AA) is characterized by pancytopenia and a hypocellular bone marrow from immune-mediated bone marrow destruction.
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PMC11629461
Further, SALPC photodynamic action triggered persistent calcium oscillations in DUOX2‐CHO‐K1 cells (Figure 6), similar to those observed in NOX5‐CHO‐K1 cells (Figure 3).
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PMC9429973
Our findings highlight that specific CD8+ T cells against SARS-CoV-2 might be induced by vaccination but do not correlate positively with serological responses.
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PMC9118379
Adopting proteogenomics approach to validate single nucleotide variation events by identifying corresponding single amino acid variant peptides from mass spectrometry (MS)-based proteomics data facilitates translational and clinical research.
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PMC11806182
Differential miRNA expression was analyzed using the R software to preprocess and normalize the data.
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PMC9429973
The unadjusted median duration of 2L for the 3 cohorts was of 6.0, 8.1 and 9.9 months (log rank test, p=0.01).
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PMC11394730
The cell cycle is a series of events that take place inside the cell in order to divide into 2 separated identical daughter cells.
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PMC11144382
Nuclear factor I/X (NFIX) mutations are associated with 2 skeletal dysplasias, Marshall-Smith (MSS) and Malan (MAL) syndromes.
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PMC11474209
The most typical cyanotoxin among the ten analogs is NOD-R. NODs were first discovered in cyanobacteria's freshwater and coastal environments, known as Nodularia .
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PMC10376064
These results prompted us to evaluate DMF at more advanced pathological stages of β-amyloidosis in this model, as several differences between early- and late-stage amyloidosis mice exist (e.g., the integrity of BBB, the regulation and function of BBB-located proteins, or altered CNS penetration of drugs and/or metabolites amongst other factors).
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PMC9429973
At diagnosis, most pts had higher-risk MDS with IPSS-R high (29%) or very high (39%) disease, and complex karyotype was seen in 44%.
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PMC11566417
Vimentin, aSMA and CK14 represent basal markers, and CK18 and ERα are luminal markers.
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PMC9910796
Interestingly, the cryo-EM structure of faustovirus has unveiled an internal icosahedral capsid layer (besides the outer one) but not an interposed internal lipid membrane .
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PMC10728535
The expression of this gene is inversely related with drug resistance, which confirms the results of our study.
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PMC10958426
UMAP visualization of 101,065 cells from tumor tissue and normal colorectal tissue, colored by tissue. (
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PMC9786247
The public health risk in Belgium was linked to specific genomic groups, which were mostly found in small ruminant strains .
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PMC11200978
Culture medium was then added to neutralize TrypLE, and the detached cell population was added to their respective 15 mL tube (pooled population).
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PMC11409031
Single-cell RNA sequencing is a revolutionary technology that has significantly enhanced our understanding of the diversity and behavior of cellular transcriptomes across various organisms.
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PMC9429973
According to the naive ITC, fedratinib was numerically favored over pacritinib in terms of SVR ≥ 35% (RR, 1.67 [95% CI, 0.94–2.97]).
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PMC11363012
40-enzyme biosynthetic pathway of the anticancer drug vinblastine (15) (Figure 1a), a natural product that is produced in Catharanthus roseus (Madagascar periwinkle), occurs in only three types of leaf cells: the first part of this alkaloid biosynthetic pathway is found in internal phloem-associated parenchyma (IPAP) cells, the middle module of the pathway occurs in epidermal cells, and finally, the last steps occur in idioblast/laticifer cells.
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PMC10968586
THP-1 is probably the most known acute monocytic leukemia cell line.
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PMC11402217
1 × 10ˆ5 143B and MG63 cells were seeded in a 12-well plate at 70–80% confluence, followed by 0.5 μM XD23 treatment for 24 or 48 h. Cells were lysed with TRIZOL (Thermo Fisher, US), reverse transcribed using PrimeScript RT Master Mix (TaKaRa, RR036A#, JP), and RT-qPCR was performed on a LightCycler96 with TB Green dye (TaKaRa, RR820A#, JP), using primers listed in Table S1.
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PMC9592219
The primary antibodies were used for the analysis after incubating the proteins with the following secondary antibodies: rabbit anti-TOP2A (1 : 2,500; CST, 12286), rabbit anti-E-cadherin (1 : 10,000) (ab40772), rabbit anti-N-cadherin (1 : 10,000) (ab18203), rabbit anti-vimentin (1 : 5,000; Abcam ab92547), rabbit anti-Snail1 (1 : 1,000; Boster, PB0449), rabbit anti-Snail2 (1 : 2,000; Boster, PB9439), rabbit anti-β-actin (1 : 10,000; Boster, BM0627), and rabbit anti-GAPDH (1 : 2,000; Boster, A00227-1).
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PMC11543885
Clarify the samples via centrifugation at 10,000 g for 10 min at 4°C to remove the cell debris.
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PMC9964635
Because of its low bioavailability of 38–49%, Sorafenib necessitates a significant daily dose in cancer therapy.
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PMC11502327
Of the drug release days included in the calculation, a total of 70% of CPT was released from the hydrogel within 18 days, and the drug delivery system maintained a good anti-tumor effect even at lower drug concentrations.
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PMC11487720
α-Tubulin was shown in the bubbles (right panel; red arrows) (D).
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PMC11013014
The binding affinity values of 2 and 3 are −6.7 kcal/mol and 7.0 kcal/mol, respectively.
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PMC11742231
Ultimately, a fully connected layer is employed to predict the response of the drug–cell interaction.
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PMC9429973
The median PFS was 13.4 months (95% CI: 11.1-16.7), with 6-month and 12-month PFS rates of 78.7% (95% CI: 67.5-86.3) and 53.1% (95% CI: 40.3-64.3).
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PMC10969097
Although participant recruitment is closed, no results are yet available.
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PMC9599726
The ferroptosis database used for the analysis was retrieved on http://www.zhounan.org/ferrdb/ (accessed on 19 September 2022).
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PMC11387401
Significant correlations are indicated with asterisks and p values are derived from the Spearman correlation analysis, all correlation results and p values can be found in Appendix Table S5 (p < 0.05:*, p < 0.001: **, and p < 0.0001: ***).
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PMC7189327
These datasets would be used for the following integrated analyses, which consisted of 48 BCC tissues and 85 normal tissue samples.
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PMC9429973
In fact, most of imatinib side effects are moderate and subside over time.
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PMC11377827
Rabbit IgG was used as a control for non-specific binding.
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PMC11585994
Primary cells from de novo T-ALL patients (n = 3) were treated with G5, G5-siNC, G5-sgc8, G5-siBCL11B, and G5-sgc8-siBCL11B nanoparticles for 48 hours, and the percentage of apoptotic cells was detected by flow cytometry (left panel), with quantitative analyses of the apoptosis rate shown in the right panel. (
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PMC9429973
Methods: Adults with R/R LBCL after first-line treatment were eligible.
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PMC8097906
Fig. 3VSM treatment effects on RH-30 cell morphology.
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PMC11473843
Mutant KRAS: mutKRAS; n = 3. (
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PMC11248911
VEGF which is secreted by tumor cells binds to and activates its receptor VEGFR2, promoting vascular growth and supplying the hypoxic areas of tumor tissues with oxygen and nutrition.
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PMC6948907
This yields purple formazan crystals that detected colorimetrically at 570 nm.
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PMC11049294
To assess the AGAL activity, 1 × 10 Jurkat cells were seeded into 96-well plates and incubated for different times (5 min, 1 h, 3 h, 5 h, 6 h, 8 h, 16 h and 24 h) with a substrate working solution (final concentration in 2.83 nmol/mL) at 37 °C in the dark, in 96-well plates.
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PMC11585565
The homozygous Cnnm4-knockout mice exhibited low serum and urine magnesium levels and a high fecal magnesium level, compared with those in wild-type mice, indicating that Cnnm4 plays an important role in magnesium absorption.
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PMC11594806
Connexin43 (Cx43), a gap junction protein facilitating intercellular communication, suppresses the proliferation of human OS U2OS cells by inhibiting the G1-to-G2 cell cycle transition.
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PMC6267596
To identify polyploidy specific proteins responsible for DNA repair, DNA replication and immune response, Log2 fold change and -Log10 P-value were co-visualized in a volcano plot for each gene using DIAP cells compared to DMSO control cells (Figure 3e).
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PMC3995603
Plasma samples were collected after centrifugation at 10000 rpm and stored at -80°C until they were further assayed.
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PMC11770746
All reagents were purchased from Biological Industries.
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PMC9243326
Families and numbers of the top 10 TFs produced by SMRT. (
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PMC11637716
SBDS‐sequencing analysis revealed a wild‐type sequence.
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PMC2193049
Implication of these findings on the modulatory effect of proteasome inhibitor on the generation of peptides presented by HLA class I is discussed.
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PMC9878278
In brief, the siRNA-Lipofectamine RNAiMAX complexes (siRNA in 50 μl of Opti-MEM medium mixed with 3 μl of Lipofectamine RNAiMAX) were added to each well and mixed gently.
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PMC11661040
Cell counting kit-8 (CCK-8) assay, colony formation assay, flow cytometry, wound healing assay, and Transwell assay were conducted for in vitro functional experiments.
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PMC10502403
Metabolomic analysis of NGMCs and NE1-GMCs Three samples from three different healthy donors per group were assessed.
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PMC11098378
As a positive control for arbovirus rescue, siRNAs targeting transcripts encoding argonaute-2 (AGO2), which we have shown to restrict VSV and SINV replication in LD652 cells , were also transfected into cells.
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PMC9268183
It is however, worth mentioning that in some cases, the informants use local euphemisms to describe the origin of the illness, such as “mal dolent” or “mal lleig”, which literally translate to bad or ugly illness .
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PMC11026382
Folate, the DHFR substrate is indicated with yellow sticks.
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PMC9429973
No differences in MRD rates were seen between treatment arms, and data was pooled for analysis.
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PMC9429973
Prompt evaluation and appropriate treatment is necessary.
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PMC11201989
Our results suggest that PD-L1 is a new player in ciliogenesis, contributing to PC-2-mediated sensory signaling and the Hh signaling cascade.
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PMC11733919
Nonsenescent normal (BJ, WI-38) and cancer (A431) cells were treated with the drugs 17-AAG and 17-DMAG in a range of concentrations starting from 0.1 to 10 μM or the nanoplatforms (60 μg of Fe/mL) for 24 h, and metabolic activity was then assayed using MTT test.
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PMC11657695
B Gene expression (Log2 normalized expression) of KCNA2 in biopsies originated from patients with different tumours (Kruskal–Wallis and Dunn’s tests).
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PMC10729469
E. MHCII-IA.-KLVFFAEDVGSNKGA (Aβ T cell epitope) tetramer binding confirming the Aβ reactivity of engineered TCRAβ-Tregs incubated with Aβ-tetramer (blue) compared to TCRAβ-Tregs incubated with control-tetramer (orange) or polyclonal Tregs incubated with Aβ-Tetramer (red) Generation of TCRAβ-Tregs.
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PMC9429973
M. S. Davids, J. Richter, C. Anderson-Smits, M. Kamieniak, K. Ren, M. Hull, J. Multani, D. Shah, C. Siffel Dana-Farber Cancer Institute, Boston; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York; Takeda Development Center Americas, Inc., Lexington, United States of America; Takeda Pharma Sp.z.o.o.,
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PMC9024365
Co-localization of SD4 with synaptic GluA1 is increased after chemical-LTP. (
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PMC11092382
Scattered studies have indicated that sophocarpine may protect other vital organs, such as the liver, kidney, brain, and lung.
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PMC11271278
These data together suggest that cancer-type specific enhancers may be subject to translocations to activate oncogene expression.
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PMC11802929
c Radar graphs of physicochemical properties for representative PG-TCR groups.
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PMC11422497
P < 0.05, **P < 0.01, na: no statistical significance.
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PMC10669128
Based on the established role of VEGF and its receptor VEGFR in angiogenesis, the authors tried to better clarify the role of VEGF165 in STS growth, metastasis, and chemoresistance.
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PMC11337594
This distinction may prove valuable in future studies of apoptosis and pyroptosis.
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PMC11794847
Then, we used PMA and Ionomycin to activate the Jurkat cells.
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PMC9429973
On the contrary, age and ELN17 genetic risk were significantly associated with OS (Table).
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PMC9429973
Median follow up was 3.1 months.
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PMC11522251
D HCT116 and CACO2 cells with knockdown of HOXB8 were dealed with distinct concentrations of cetuximab (0, 100, 300 μg/ml), and their cell migration ability was determined. ***
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PMC10560648
Each experiment was repeated three times (triplicates).
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PMC9436459
The relative configurations of 139a and 141a were the same as those of natrual MDAAs palodesangrens B and D (139 and 141).
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PMC10055960
For example, ligands bearing extended π-electron systems have been shown to bind to DNA via intercalation .
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