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OTAR3088
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PMC9635307
Primary B cells isolated from B6 SPL were stimulated with either LPS, agonistic anti-CD40 or anti-Igμ F(ab’)2 fragment, to activate Toll-like receptor (TLR) 4, CD40, or B cell receptor (BCR) signaling, respectively, in the presence or absence of an agonistic CEACAM1 mAb, CC1.
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PMC8524093
The role of each of the CHIKV proteins is summarized in Table 1.
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PMC10654497
Form for clinical information.
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PMC6901583
This was followed by a double phosphate-buffered saline (PBS) wash and overnight fixation utilizing 70% ethanol at −20 °C.
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PMC11258145
The ratios of saturated to unsaturated fatty acids in solutions S to US were: 48–52% for SK-N-SH and for HL-60: 34.6% S to 65.4% US.
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PMC11756514
Furthermore, it has previously been described that purified peptidoglycan from S. aureus is a target of SP-D (49).
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PMC9532503
In ADCC, an antibody first binds to a cell-surface target antigen, then recruits immune effector cells that lyse the target cell.
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PMC10674574
Moreover, validation of the Caco-2 cell line used for pharmaceutical purposes is performed for a single passage number .
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PMC11723210
The LED matrix contains five quasi-monochromatic LEDs emitting wavelengths of 466 nm, 521 nm, 588 nm, 607 nm, and 632 nm, with bandwidths ranging from 20 to 50 nm.
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PMC9429973
Seven adults had a history of anaphylaxis.
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PMC9250505
Indeed, it is reported that TP53 and BAD protein co-operatively promote apoptosis subsequent to DNA damage in cancer cells.
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PMC11519788
These findings highlighted an inverse correlation between SPOP levels and UBC aggressiveness, suggesting its potential as a prognostic biomarker for UBC patients.
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PMC9910796
The top right inset shows the similarity between the core shell of an assembling particle (blue outline) and a zipper structure (red outline). (
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PMC11126803
Unless otherwise stated, all results are expressed as the mean ± SD, and the experiment was independently repeated at least 3 times with data points in triplicate.
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PMC11394730
Moreover, the emodin-treated SMMC-7721 cells exhibited a substantial upregulation of cleaved caspase-3 and -9 expression levels.
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PMC11765879
The cells were also treated with an equivalent concentration of dimethyl sulfoxide (DMSO), and it had no inhibitory effect on the cell viability of both cell lines.
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PMC9429973
The most frequently observed TRAEs were anemia (15 patients,78.9%), neutropenia (14 patients, 73.6%), leukopenia(11 patients, 57.9%), nausea (10 patients, 52.6%), thrombocytopenia (9 patients, 50%).
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PMC11085211
Proteins were separated in SDS-PAGE (12%) and transferred onto nitrocellulose membranes.
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PMC11629192
Patient 2: A 12-year-old male exhibited anti-NMDAR encephalitis with high-frequent seizures occurring every 2–3 minutes, leading to status epilepticus that required invasive mechanical ventilation and continuous intravenous administration of midazolam.
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PMC11201989
Furthermore, we show an association between PD-L1 and BBS5, and PD-L1 and PC-2.
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PMC9587866
HIF1-alpha is implicated in mediating tumor cell evasion by inhibiting apoptotic factors, and immunological influence (El-Naggar et al., 2015) and also provide chemotherapy resistance.
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PMC6627640
Clones were then derived from each pool with cloning cylinders (Supplementary Table S1).
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PMC7342409
Compared with the imatinib-resistant group, there was an upward trend but no significant change (p>0.05) in the imatinib group and imatinib + CQ group, but there was a downward trend without any significant difference in the CQ group (p>0.05).
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PMC3164356
Methods for cell transfection, gene amplification and clone selection in CHO cells are well characterized and widely used.
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PMC11089031
After being sealed with paraffin, the tissue blocks were fixed on a tissue slicer and were sliced into serial sections with a 5 μm thickness.
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PMC9429973
For this reason, we must strive for the earliest and most accurate diagnosis possible.
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PMC10940855
Samples were washed with 1 mL of Pharmingen Stain Buffer (BD Biosciences, 554656).
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PMC9429973
Branched Chain Amino Acid Transferase 1 (BCAT1), a cytosolic aminotransferase converts BCAAs into their corresponding branched-chain α-keto acids and vice versa.
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PMC11451829
According to Zhao et al., the presence of conjunctival hyperemia is associated with a high rate of recurrence.
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PMC9429973
The median age was 81 years in the global cohort (range 68 to 95), with a median age of 85.5 and 78 years in the group of BSC and non-intensive treatment respectively.
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PMC10965315
In the control group, only 5.27% and 9.23% of the cells had progressed to the late and necrotic phases, respectively (Figure 2a).
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PMC10024132
The γ9δ2 T cells were administered every 3 days for a total of five treatments (total 5×10 cells).
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PMC11406030
IHC was performed on a Dako Autostainer Plus (Agilent Technologies, Santa Clara, CA, USA) using Dako EnVision FLEX visualization systems.
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PMC9895440
Cells were stimulated on the same day with 1:1 ratio of CD3/CD28 beads (Human T cell Activation and Expansion kit, Miltenyi) and 150 U/ml rh-IL-2 (Proleukin).
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PMC10818573
To evaluate the applicability of the new developed method (RPA-LFA), 192 cell lines were tested in parallel using commercial quantitative real-time polymerase chain reaction (qPCR, DaAn Gene, Guangzhou, China), traditional PCR, and RPA.
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PMC11672142
The cleavage of the substrate is measured by fluorescence (Ex/Em = 400/505 nm).
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PMC11697065
iDEP (2.01) R package analysis tool was used to perform PCA, heatmap, k-mean clustering and GO term enrichment analysis for both RNA-seq and proteomics data.
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PMC11549062
PDGF‐ PDGFR‐β signaling is reported to be associated with vascular diseases, and plays a key role in VSMCs migration and proliferation during atherosclerosis .
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PMC11748618
Significance of variant combinations was defined as log10 BF ≥ 3.5 as defined by Servins and Stephens, 2007.
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PMC9429973
Results: During april 15 and december 31 2020, 60 patients received convalescent plasma.
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PMC8345486
p values were two-sided; p ≤ 0.05 was considered statistically significant.
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PMC11657695
B Normalized and relative EWS::FLI1 and KCNA2 gene expressions in A673/TR/shEF cell line after doxycycline treatment (1 µg/mL) (N = 5, left and middle panel).
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PMC10237474
Cells were washed once with PBS and re-suspended in 1 mL of Opti-MEM.
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PMC11194678
After 4 days viral infection, cells were treated with 1,000 ng/ml doxycycline (60204ES03; YEASEN) for 1 day to induce the expression of GFP-STAU1 or GFP-STAU1 5KE.
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PMC11411131
We found that MeHg induces a truncated form of selenoproteins, including TrxR1 and other designed proteins (Figs. 1 and 4).
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PMC10222971
Surgical excision of these malignancies remains the traditional mainstay of treatment as well as topical treatments with semisolid formulations (with 5-fluorouracil, diclofenac, and imiquimod) and photodynamic therapy, among others.
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PMC9577200
The test was presented in 3 replicates.
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PMC11026382
c Comparison of the relative growth rate effects for DHFR single mutants in the WT and TYMS Q33S backgrounds.
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PMC11641532
Several common mutated genes in ccRCC have been identified, including VHL, polybromo 1 (PBRM1), SET domain containing 2, histone lysine methyltransferase (SETD2), phosphatase and tensin homolog (PTEN), and tumor protein p53 (TP53) .
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PMC11532793
Bootstrap sampling is by far the most computationally demanding step in the analysis pipeline, consuming much more time than either the alignment or the statistical analysis.
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PMC11412721
mRNA levels of p53 target genes in MEFs untreated or treated with 0.5 μg/ml of clastogenic doxorubicin (Doxo) or 10 μM of the Mdm2 antagonist Nutlin.
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PMC9919300
The treatment with curcumin-andrographolide in association maintained the ability to regulate in a negative manner FABP1 (15.96 times), PPARGC1A (7.85 times), GK and ABCA1 (4 times), and APOC3 (2.04 times) in respect to the steatosis control.
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PMC11740907
Molecular docking is a valuable tool in the field of cancer treatment using polyoxometalate (POM) integrated solid lipid nanoparticles.
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PMC11545868
Some studies suggest that isoflavones improve cholesterol levels, reduce the risk of osteoporosis, and help with female menopause and heart disease .
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PMC9325715
Instead, the best results were obtained for aspartic acid (7.844), histidine (7.844) and tyrosine (7.844).
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PMC10813895
Emerging evidence from transcriptional profiling points to the downregulation of ELF5 as a pivotal driver of the aging phenotype in luminal epithelial progenitor cells, with potential implications for increased breast cancer susceptibility.
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PMC11621493
6PLA2G4D enhances the incorporation of MAG from extracellular sources into cellular acylglycerols independently of DGAT. (
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PMC11612315
j Confocal images of FITC-labeled exendin 9–39 in CHO K1 cells expressing human TRPV1 (upper) compared with naïve CHO K1 cells (lower).
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PMC11745823
When signalling molecules (such as growth factors) bind to cell membrane receptors (such as RTK or GPCR), PI3K (phosphatidylinositol 3‐kinase) is activated.
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PMC11757230
These differences in the regulatory mechanisms of the cell cycle among cell lines have been shown to affect their sensitivity to various inhibitors, such as the pan-CDK inhibitor flavopiridol , the CDK4/6 inhibitors palbociclib and abemaciclib , and the hydroxymethylglutaryl-coenzyme A reductase inhibitor simvastatin for inducing cell cycle arrest and cell death.
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PMC11395280
Regardless of the mechanism of CCL198-83 actions, it is evident that the use of an inhibitor of CCR7, which belongs to the large G protein-coupled receptor family that makes up about a third of all FDA-approved drugs, can be targeted with significant in vivo survival benefits.
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PMC11803210
StringTie was used to reconstruct transcripts and novel transcripts identified with Cuffcompare, a tool of Cufflinks .
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PMC11711345
Biological Targets (and Correlations) on Human Cancerous Cell Lines with High Interaction (Probability ≥ 0.7) of p-Cymene and Its 7-Oxygenated Derivatives. *
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PMC11661024
Cancer cell proliferation abnormally increases due to functional mutations in apoptosis-associated genes; thus, targeting apoptosis is one of the primary goals in non-invasive cancer treatment [28–31].
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PMC11711063
Repleting ZG16B by sequential administration restored both cell proliferation and motility to baseline levels (Figure 3C,D).
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PMC5839394
In particular the differentially proteins identified can efficiently explain the cellular effects of AgNPs-EPS.
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PMC11661040
The results from three independent experiments were presented concisely in a histogram format. (
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PMC6450504
Whether or not this microtubule polymerizing capacity of PTX caused subsequent apoptosis was also investigated using imaging laser scanning cytometry (LSC) analysis in a single end-point experiment (Figure 4(c–f)).
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PMC9031474
9-epipolygodial (49) was obtained by an epimerization reaction of polygodial (2) (Scheme 1).
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PMC9368503
The resulting digest was acidified to pH < 4 by trifluoroacetic acid (TFA), desalted on Pierce C18 Spin Columns (Thermo Scientific) according to the manufacturer’s protocol, and dried in a SpeedVac.
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PMC10421378
The intentional use of stresses may increase the resistance of cells to unfavorable conditions during drying (dehydration, osmotic stress, shear stress, elevated temperature).
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PMC9429973
Age, type of induction therapy, karyotype, presence of FLT3-ITD mutation, extramedullary involvement and type of MPAL (B/myeloid vs T/myeloid) were tested in univariate analysis for OS and DFS.
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PMC9275501
CUR could improve CDDP-induced spatial learning and memory impairment (Oz et al., 2015) and nerve oxidative damage (Ozkaya & Naziroglu, 2020).
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PMC10631132
Cell viability and cell number were measured after Trypan blue was added to testing samples.
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PMC11769522
Th17-type IL-17A, which is upregulated in human and canine AD, is reported to induce the production of pro-inflammatory cytokines by activated human keratinocytes ; therefore, in addition to IFN-γ and TNF-α, future studies could investigate whether IL-17A induces multiple cytokine production by activated canine keratinocytes.
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PMC11252033
Docking calculations resulted in a main cluster of 21 out of 50 conformations including the lowest energy one (Supplementary Table S2).
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PMC11686380
n = 11 to 28 neurons. **
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PMC11322866
In addition, blue arrows indicate swollen mitochondria lacking cristae.
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PMC6892818
As positive controls, lysates from HCT116 cells that were treated with control (‘C’) or treated with mCD40L (‘mL’) for 6 h were included.
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PMC11495567
A-B) Confluent monolayers of cancer cells separated by a “wound” generated using cell culture inserts (ibidi) were treated with the indicated concentrations of NSL-YHJ-2-27 or NSL-YHJ-2-46.
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PMC9429973
To characterize functional responses to 10 PI3Ki in CLL 2.
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PMC11026382
Next, we examined the structural pattern of DHFR positions with epistasis to TYMS Q33S and TYMS R166Q.
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PMC3734024
We therefore sought to determine whether IL-5 also affected IL-10 secretion.
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PMC8041314
Cells were stored under liquid nitrogen in medium (90%) and DMSO (10%) before culturing (5% CO2-humidified atmosphere; 37 °C).
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PMC11266100
Therefore, long-term transfection was performed utilizing nanosecond duration (7.5 kV/cm × 300 ns × 250, 1 MHz) EP protocol that resulted in a highly luminescent clone of the 4T1 cell line, which was subsequently tested in an in vivo murine model.
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PMC11721064
scale bar = 50 μm) To isolate LNCs, limbal segments were subjected to enzymatic digestion using dispase II and collagenase.
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PMC11770130
Ntsr2 depletion efficiency and specificity in the adipocytes and adipose tissues were validated by RT-qPCR (Fig. 1c; Supplementary information, Fig. S2a, b).
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PMC10213179
Aptamers were then added to the cells at a concentration of 200 nM and incubated for 1 h at 37°C, gently swirling every 30 min.
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PMC9429973
Asciminib is a recently approved drug that inhibits the BCR::ABL1 oncoprotein through a novel mechanism of action: Specifically Targeting the ABL Myristoyl Pocket (STAMP).
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PMC11409031
H–K) Alterations in the transcription of P21, BAX, CDH1 and CDH2 following inhibition of PFN1 in the A2780 cell line. (
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PMC10362265
This region encompasses the residues between 68 and 81 that are either unfolded or completely missing in all known IL18-IL18BP complexes , , .
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PMC11537649
Furthermore, Lnc NEAT1 facilitated proliferation, migration, and invasion of melanoma cells.
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PMC11575040
Previous studies have reported many potential links of genes, miRNAs, and lncRNAs with the progression and survival rate of melanoma, as well as a potential pathway involving metformin and melanoma.
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PMC11482552
The internationally recognized criteria for the validation of HPV tests to be used in cervical cancer screening programs have focused on the importance of assuring the clinical sensitivity and specificity of the assays for the detection of women with precancerous lesions, but less attention has been placed on sample adequacy assessment .
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PMC9024365
For analysis of puncta size based on co-localization with SD4 (stratification analysis), co-localization was defined as overlap of ≥ 1 pixel.
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PMC10957991
Bar 200 um. (
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PMC11695546
Many studies showed the anticancer impact of coconut oil (Verma et al. 2019; Alotaibi et al. 2023).
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PMC11200978
When samples were treated with a repeat freeze to −15 °C, a significant decline in cell viability was observed at 24 h post treatment compared to −15 °C single freeze samples (D1: repeat −15 °C: 22.7% (±1.2) vs. single −15 °C: 96.4% (±2.8), p < 0.01); however, the surviving cells were able to recover in vitro over the 7-day assessment period (D7: 132.9% (±3.4)).
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PMC11224020
Only immature DCs confined at a height of 3 μm exhibited a significant increase in CCR7 expression at both mRNA and protein levels (Fig. 1e,f).
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PMC10440586
We inferred the tumor purity and stromal and immune cell admixture using the ESTIMATE algorithm .
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